Faculty Profiles - TAKEDA KAZUKI

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TAKEDA KAZUKI

Organization

School of Computing Specially Appointed Associate Professor (Lecturer)

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DVM, Ph.D.(Veterinary Sciences), Environmental Toxicologist

Solving environmental problems is an international challenge that is included in the Sustainable Development Goals (SDGs).
To address them, I am challenging to elucidate the effects of environmental pollutants on humans and wildlife by using my knowledge of animal science as a veterinarian and the latest molecular biology and simulation methods.
Currently, I am working on the development of non-invasive evaluation methods using simulation methods, especially for rare species where animal experiments cannot be conducted.

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Degree

Ph.D. (Veterinary sciences) ( 2020.3 Graduate School of Veterinary Medicine, Hokkaido University )

Research Interests

SDGs
Analytical chemistry
Pesticide
QSAR
Drug metabolism
Rodenticide
Wildlife
Bioinfomatics
Pharmacokinetics
Environmental Chemical
Cytochrome P450
Environmental Polution
Machine Learning
Computational Science
Computational Toxicology
Toxicology

Research Areas

Environmental Science/Agriculture Science / Chemical substance influence on environment / Environmental Chemistry
Life Science / Veterinary medical science / Veterinary Toxicology
Informatics / Life, health and medical informatics / Computational Biochemistry
Life Science / Structural biochemistry

Education

Hokkaido University Graduate School of Veterinary Medicine

2016.4 - 2020.3

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Country： Japan

Notes： Chemical hazard control expert

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Hokkaido University School of Veterinary Medicine

2010.4 - 2016.3

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Country： Japan

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Research History

Institute of Science Tokyo School of Computing Specially Appointed Associate Professor JST BOOST Program

2025.9

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Kitasato University School of Veterinary Medicine Lecturer/Junior Associate Professor

2023.4

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Country：Japan

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Kitasato University School of Veterinary Medicine Assistant Professor

2020.9 - 2023.3

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Country：Japan

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Tokyo Institute of Technology School of Computing JSPS Superlative Postdoctoral Fellow

2020.4 - 2020.8

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Country：Japan

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Hokkaido University Graduate School of Veterinary Medicine Research Fellowship for Young Scientists, Japan Society for the Promotion of Science

2017.4 - 2020.3

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Hokkaido University Faculty of Veterinary Medicine Attending Veterinarian

2016.4 - 2020.3

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Country：Japan

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Hokkaido University Graduate School of Veterinary Medicine

2016.4 - 2017.3

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Professional Memberships

Society of Toxicology

2025.9

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The Japanese Society of Veterinary Science

2025.4

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The Vitamin Society of Japan

2025.4

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Society of Environmental Toxicology and Chemistry

2020.4

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The Japanese Society of Toxicology

2016.4

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Japan Society for Environmental Chemistry

2015.4

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Committee Memberships

Specialty Section on Adduct Sciences, Japanese Society of Toxicology Chair of the Organizing Committe

2025.10

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Committee type：Academic society

https://www.senkyo.co.jp/adduct/index.html

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Japanese Society of Toxicology Councilor

2025.7

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Committee type：Academic society

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Specialty Section on Adduct Sciences, Japanese Society of Toxicology Executive Committee Member

2025.7

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Committee type：Academic society

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Japan Society for Environmental Chemistry Councilor

2025.4

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Committee type：Academic society

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The 52nd Annual Meeting of the Japanese Society of Toxicology Organizing Committee

2024.7 - 2025.7

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Committee type：Academic society

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4th Joint Meeting of Environmental Chemicals (33rd Annual Meeting of the Society of Environmental Chemistry and the 29th Annual Meeting of the Society of Environmental Toxicology) Organizing Committee Member

2024.7 - 2025.7

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Committee type：Academic society

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Digital Toxicology Conference Organizing Committee

2023.10 - 2024.7

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Committee type：Academic society

https://www.jsot2024.jp/dtc/index.html
https://peatix.com/event/3872259

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The 51st Annual Meeting of the Japanese Society of Toxicology Women and Young Researcher Program Committee

2023.7 - 2024.7

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Committee type：Academic society

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Papers

Isomer-Dependent Pharmacokinetic Behavior and VKOR Interactions of Second-Generation Anticoagulant Rodenticides: An Integrated In Vivo–In Vitro–In Silico Investigation Invited Reviewed

Moyu Miyamae, Satoru Nagaoka, Teppei Hayama, Misaki Fukamatsu, Ryo Kamata, Kazuki Takeda

International Journal of Molecular Sciences 27 ( 9 ) 3794 - 3794 2026.4

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Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Second-generation anticoagulant rodenticides (SGARs) were developed to overcome warfarin resistance in rodent populations; however, their prolonged hepatic retention has raised concerns regarding secondary poisoning of non-target wildlife. All major SGARs exist as cis–trans isomeric pairs, and differences in biological half-life between isomers have been reported, yet the molecular basis for such isomer-dependent pharmacokinetic behavior remains poorly understood. In this study, we conducted an integrated evaluation of cis and trans isomers of SGARs using in vivo, in vitro, and in silico approaches, with vitamin K epoxide reductase (VKOR) serving as the molecular target. The individual compounds exhibited distinct isomer-dependent profiles in hepatic retention, inhibitory potency (IC50), and VKOR interaction-related properties. Molecular dynamics simulations further revealed isomer-dependent differences in torsional flexibility around specific rotatable bonds and in ligand–VKOR interaction fractions. For flocoumafen and bromadiolone, the presence of an ether oxygen was associated with increased torsional and orientational flexibility and enhanced hydrogen-bonding potential, which may facilitate metabolic processing and contribute to the relatively faster elimination of cis isomers. Collectively, these results suggest that isomer-specific VKOR interaction patterns may contribute, in a compound-dependent manner, to isomer-dependent pharmacokinetic behavior, offering structural perspectives for the design of rodenticides with reduced ecological risk.

DOI： 10.3390/ijms27093794

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Perfluorohexanesulfonic acid-induced inhibition of human palate cell proliferation through upregulation of miR-374a-5p Reviewed

Hiroki YOSHIOKA, Hanane HORITA, Kenichi OGATA, Kazuki TAKEDA, Hyogo HORIGUCHI, Yosuke TSUKIBOSHI

Biomedical Research 47 ( 1 ) 1 - 10 2026.1

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Publishing type：Research paper (scientific journal) Publisher：Biomedical Research Press

DOI： 10.2220/biomedres.47.1

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A New Class of Vitamin K Analogues Containing the Side Chain of Retinoic Acid Have Enhanced Activity for Inducing Neuronal Differentiation Reviewed

Yoshihisa Hirota, Taiki Sato, Rina Watanabe, Kazuki Takeda, Sho Sano, Satoshi Asano, Yuki Shibahashi, Yumi Yasuda, Yuta Takagi, Yutaro Yamashita, Wu YuXin, Mikino Arakawa, Yuri Maitani, Vannessa Lawai, Kurumi Nakagawa, Natsuko Furukawa, Atsuko Takeuchi, Chisato Tode, Maya Kamao, Akimori Wada, Zainab Ngaini, Yoshitomo Suhara

ACS Chemical Neuroscience 16 2812 - 2828 2025.7

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acschemneuro.5c00111

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Development and Mechanistic Evaluation of a Lithocholic Acid Derivative as an Environmentally Safe Rodenticide Reviewed

Kana Okamura, Kazuki Takeda, Satoru Nagaoka, Chiharu Ogura, Misaki Fukamatsu, Mayumi Degawa, Hiroyuki Kagechika, Kenji Ohgane, Ryo Kamata, Aya Tanatani

ACS Pharmacology & Translational Science 2025.7

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acsptsci.5c00059

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Comprehensive molecular docking on the AlphaFold-predicted protein structure proteome: identifying target protein candidates for puberulic acid Reviewed

Teppei Hayama, Rin Sugawara, Ryo Kamata, Masakazu Sekijima, Kazuki Takeda

The Journal of Toxicological Sciences 50 ( 7 ) 309 - 324 2025.7

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Toxicology

DOI： 10.2131/jts.50.309

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Protective function of the voltage-gated potassium channel Kv11.3 in a mouse model of cardiac ischemia/reperfusion injury Reviewed

Hayato Sasaki, Kazuki Otake, Kazuki Takeda, Karin Tesaki, Eiki Takahashi, Jumpei Yasuda, Shizukaze Matsuda, Ayumu Kawasaki, Masaki Watanabe, Kosuke Otani, Muneyoshi Okada, Masakazu Sekijima, Hideyuki Yamawaki, Nobuya Sasaki

PLOS One 20 ( 5 ) e0323428 - e0323428 2025.5

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Language：English Publishing type：Research paper (scientific journal) Publisher：Public Library of Science (PLoS)

Voltage-gated potassium (Kv) channels contribute to repolarization in excitable tissues such as nerves and cardiac muscle; consequently, they control the firing frequency and duration of action potential. Their dysfunction can thus cause neurological disorders and cardiac disorders with arrhythmias. The dysfunction of Kv11.3 is associated with bipolar disorder, but no reports have linked it to heart disease. Kv11.3-knocked out (KO) mice exhibit behavioral abnormalities, but they do not have cardiac abnormalities. Ischemia–reperfusion (I/R) experiments were performed on the hearts of Kv11.3 KO mice to determine whether they would differ from wild-type mice when exposed to stimuli that could induce sudden cardiac death. The mortality rates and infarct size of the Kv11.3 KO mice increased after cardiac I/R. The corrected QT interval was shortened in the wild-type mice after cardiac I/R, but it remained nearly unchanged in Kv11.3 KO mice with alterations in heart rate variability. These phenotypes could be reproduced by administering high-dose NS-1643, a Kv11.3 channel antagonist, after cardiac I/R. The infarct size had no significant difference in the ex vivo cardiac I/R experiment in contrast to the in vivo cardiac I/R experiment. Our study indicated that Kv11.3 protects the myocardium from I/R injury through neural pathways.

DOI： 10.1371/journal.pone.0323428

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Comprehensive Molecular Docking on the AlphaFold-Predicted Protein Structure Proteome: Identifying Target Protein Candidates for Puberulic Acid, a Suspected Lethal Nephrotoxin

Teppei Hayama, Rin Sugawara, Ryo Kamata, Masakazu Sekijima, Kazuki Takeda

BioRxiv 2025.2

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Authorship：Last author,　Corresponding author Language：English

DOI： 10.1101/2025.01.30.635827

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Establishment of an evaluation method of a chemical-susceptibility for wildlife using counter-QSAR and machine learning Invited International journal

Kazuki Takeda

Impact 2024 ( 2 ) 18 - 19 2024.10

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Language：English Publishing type：Research paper (scientific journal) Publisher：Science Impact, Ltd.

Certain environmental chemicals are harmful to lifeforms. Determining the extent to which they are damaging to animals is challenging as it is ethically reprehensible to expose animals to lethal doses of chemicals in the lab and, besides, not every species of animal can be housed in a laboratory. So, how can experiments involving animals in their natural habitat, and without causing harm, be conducted? A team of researchers at the Laboratory of Toxicology in the School of Veterinary Medicine at Kitasato University in Japan, which includes Junior Associate Professor Kazuki Takeda, is working to develop a way to determine the susceptibility of animal species to given environmental chemicals. Takeda is applying his background in environmental toxicology to the research. The focus for the team is on wild animals and using non-invasive methods. As there is such great interspecies variability in chemical sensitivity, it is difficult to extrapolate toxicity test results obtained from laboratory animals to wild animals. Therefore, the researchers are using genetic information and protein-chemical binding simulations to evaluate interspecies differences in chemical sensitivity. The goal is to develop a model that can predict chemical sensitivity differences between species using simulation methods and machine learning using DNA information obtained from hair.

DOI： 10.21820/23987073.2024.2.18

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Assessment of the Aryl Hydrocarbon Receptor–Mediated Effects of Aromatic Sensitizers in Paper Recycling Effluent Employing Zebrafish Embryos and in Silico Docking Reviewed International journal

Kazuki Takeda, Aoi Sarata, Masanori Terasaki, Akira Kubota, Keita Shimizu, Ryo Kamata

Environmental Toxicology and Chemistry 2024.8

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Aromatic sensitizers and related substances (SRCs), which are crucial in the paper industry for facilitating color‐forming and color‐developing chemical reactions, inadvertently contaminate effluents during paper recycling. Owing to their structural resemblance to endocrine‐disrupting aromatic organic compounds, concerns have arisen about potential adverse effects on aquatic organisms. We focused on SRC effects via the aryl hydrocarbon receptor (AHR), employing molecular docking simulations and zebrafish (Danio rerio) embryo exposure assessments. Molecular docking revealed heightened binding affinities between certain SRCs in the paper recycling effluents and zebrafish Ahr2 and human AHR, which are pivotal components in the SRC toxicity mechanism. Fertilized zebrafish eggs were exposed to SRCs for up to 96 h post fertilization; among these substances, benzyl 2‐naphthyl ether (BNE) caused morphological abnormalities, such as pericardial edema and shortened body length, at relatively low concentrations (1 μM) during embryogenesis. Gene expression of cytochrome P450 1A (cyp1a) and ahr2 was also significantly increased by BNE. Co‐exposure to the AHR antagonist CH‐223191 only partially mitigated BNE's phenotypic effects, despite the effects of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin being relatively well restored by CH‐223191, indicating BNE's AHR‐independent toxic mechanisms. Furthermore, some SRCs, including BNE, exhibited in silico binding affinity to the estrogen receptor and upregulation of cyp19a1b gene expression. Therefore, additional insights into the toxicity of SRCs and their mechanisms are essential. The present results provide important information on SRCs and other papermaking chemicals that could help minimize the environmental impact of the paper industry. Environ Toxicol Chem 2024;00:1–13. © 2024 SETAC

DOI： 10.1002/etc.5969

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Exploring 2-methyl–substituted vitamin K3 derivatives with potent inhibitory activity against the 3CL protease of SARS-CoV-2 Reviewed

Ryohto Koharazawa, Mayu Hayakawa, Kazuki Takeda, Kotone Miyazaki, Chisato Tode, Yoshihisa Hirota, Yoshitomo Suhara

Bioorganic & Medicinal Chemistry Letters 100 129642 - 129642 2024.3

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.bmcl.2024.129642

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Sensitivity assessment of diphacinone by pharmacokinetic analysis in invasive black rats in the Bonin (Ogasawara) Archipelago, Japan Reviewed

Kazuki Takeda, Keita Shimizu, Miyu Sato, Masafumi Katayama, Shouta M.M. Nakayama, Kotaro Tanaka, Yoshinori Ikenaka, Takuma Hashimoto, Ryuichi Minato, Yusuke Oyamada, Kazuyuki D. Tanaka, Goro Kimura, Tsutomu Tanikawa, Keisuke Kato, Taichi Kusakabe, Mayumi Ishizuka, Ryo Kamata

Pesticide Biochemistry and Physiology 199 105767 - 105767 2024.2

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.pestbp.2023.105767

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Toxicity Assessment of Mixed Exposure of Nine Perfluoroalkyl Substances at Concentrations Relevant to Daily Intake Reviewed

Kazuki Takeda, Taki Saito, Sakura Sasaki, Akifumi Eguchi, Makoto Sugiyama, Saeka Eto, Kio Suzuki, Ryo Kamata

Toxics 12 ( 1 ) 52 2024.1

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal)

DOI： 10.3390/toxics12010052

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Evaluation of anticoagulant rodenticide sensitivity by examining in vivo and in vitro responses in avian species, focusing on raptors Reviewed

Kraisiri Khidkhan, Fuyu Yasuhira, Aksorn Saengtienchai, Chaiyan Kasorndorkbua, Ratiwan Sitdhibutr, Kohei Ogasawara, Hikaru Adachi, Yukiko Watanabe, Keisuke Saito, Hidefumi Sakai, Kazuo Horikoshi, Hajime Suzuki, Yusuke K. Kawai, Kazuki Takeda, Yared B. Yohannes, Yoshinori Ikenaka, Barnett A. Rattner, Mayumi Ishizuka, Shouta M.M. Nakayama

Environmental Pollution 122837 - 122837 2023.11

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Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.envpol.2023.122837

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Subchronic Toxicity Assessment of Perfluoroalkyl Substances by Mixed Exposure of Nine Substances at Daily Intake Relevant Concentration

Kazuki Takeda, Taki Saito, Sakura Sasaki, Akifumi Eguchi, Makoto Sugiyama, Kio Suzuki, Saeka Eto, Ryo Kamata

BioRxiv 2023.5

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Authorship：Lead author,　Corresponding author Language：English Publisher：Cold Spring Harbor Laboratory

Abstract

Per- and poly-fluoroalkyl substances (PFAS) have been utilized extensively for various applications owing to their distinctive chemical properties. They exhibit high persistence in the environment and accumulate within the human body, necessitating toxicity assessments. However, the consequences of prolonged, low-level exposure to PFAS and concurrent exposure to multiple PFAS have not been explored. In this study, male C57BL/6J mice (aged 8 weeks) were exposed to a composite of nine PFAS, which include long-chain PFAS (e.g. perfluorooctanoic acid and perfluorooctanesulfonic acid) and short-chain PFAS (e.g. perfluorobutanoic acid and perfluorobutanesulfonic acid) at concentrations equivalent to the estimated daily human intake in the composition reported (1 µg/L [sum of the nine compounds], the maximum reported exposure concentration) via drinking water. Histological examination revealed vacuolization of hepatocytes and irregular arrangement of hepatocyte cords, suggesting that exposure to low levels of the PFAS mixture causes morphological changes in liver tissues. Transcriptome analysis revealed that PFAS exposure mainly altered a group of genes related to metabolism and chemical carcinogenesis. Machine learning analysis of the liver metabolome showed a typical concentration-independent alteration upon PFAS exposure, and in addition to known substances such as glutathione, a compound with unknown biological function; 2,5-dihydro-2,4-dimethyloxazole was found. This study demonstrates that daily exposure to PFAS leads to morphological changes in liver tissues and alters the expression of metabolism-and cancer-related genes as well as phospholipid metabolism. Future studies are required to evaluate the chronic toxicity of prolonged, low-level exposure to PFAS mixtures and to investigate the health effects of PFAS.

DOI： 10.1101/2023.05.17.541082

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Observation of hapten-induced sensitization responses for the development of a mouse skin sensitization test, including the elicitation phase Reviewed International journal

Ryo Kamata, Yukiko Okawa, Yuto Hamaguchi, Soma Tabata, Masanori Terasaki, Kazuki Takeda

Scientific Reports 12 ( 1 ) 19898 - 19898 2022.11

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Authorship：Last author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

Abstract

The only official method that can detect the skin sensitizing potential of chemicals, including the elicitation response, is the OECD test guideline (TG) 406. However, this guideline uses guinea pigs, which requires complex procedures. Since a simple and complete test method for evaluating skin sensitization is needed, especially for mechanistic studies of skin sensitization, this study confirmed the reactivity of mice to skin sensitizing substances. We set up a protocol involving one induction exposure of the test substance to the back skin, followed by three challenge exposures to the auricle (Protocol 2), and compared their skin sensitization responses with the results of two exposures to the auricle and back skin every 2 weeks (Protocol 1) and a local lymph node assay (TG442B). A hapten 2,4-dinitrofluorobenzene caused significant auricular thickening, skin inflammation, and enlarged auricular lymph nodes in Protocols 1 and 2. These changes were more pronounced in Protocol 2. Plasma IgE and IgG1 and gene expression of IL4, IFNγ, and perforin were significantly increased in Protocol 2. Cell proliferation in the auricular lymph nodes was observed in both protocols as in TG442B. These results indicate that Protocol 2 can be a good candidate for a relatively simple skin sensitization test.

DOI： 10.1038/s41598-022-24547-1

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Other Link： https://www.nature.com/articles/s41598-022-24547-1
Toxicokinetic analysis of the anticoagulant rodenticides warfarin & diphacinone in Egyptian fruit bats (Rousettus aegyptiacus) as a comparative sensitivity assessment for Bonin fruit bats (Pteropus pselaphon) Reviewed International journal

Kazuki Takeda, Kosuke Manago, Ayuko Morita, Yusuke K. Kawai, Nobuaki Yasuo, Masakazu Sekijima, Yoshinori Ikenaka, Takuma Hashimoto, Ryuichi Minato, Yusuke Oyamada, Kazuo Horikoshi, Hajime Suzuki, Mayumi Ishizuka, Shouta M.M. Nakayama

Ecotoxicology and Environmental Safety 243 113971 - 113971 2022.9

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Anticoagulant rodenticides have been widely used to eliminate wild rodents, which as invasive species on remote islands can disturb ecosystems. Since rodenticides can cause wildlife poisoning, it is necessary to evaluate the sensitivity of local mammals and birds to the poisons to ensure the rodenticides are used effectively. The Bonin Islands are an archipelago located 1000 km southeast of the Japanese mainland and are famous for the unique ecosystems. Here the first-generation anticoagulant rodenticide diphacinone has been used against introduced black rats (Rattus rattus). The only land mammal native to the archipelago is the Bonin fruit bat (Pteropus pselaphon), but little is known regarding its sensitivity to rodenticides. In this study, the Egyptian fruit bats (Rousettus aegyptiacus) was used as a model animal for in vivo pharmacokinetics and pharmacodynamics analysis and in vitro enzyme kinetics using their hepatic microsomal fractions. The structure of vitamin K epoxide reductase (VKORC1), the target protein of the rodenticide in the Bonin fruit bat, was predicted from its genome and its binding affinity to rodenticides was evaluated. The Egyptian fruit bats excreted diphacinone slowly and showed similar sensitivity to rats. In contrast, they excreted warfarin, another first-generation rodenticide, faster than rats and recovered from the toxic effect faster. An in silico binding study also indicated that the VKORC1 of fruit bats is relatively tolerant to warfarin, but binds strongly to diphacinone. These results suggest that even chemicals with the same mode of action display different sensitivities in different species: fruit bat species are relatively resistant to warfarin, but vulnerable to diphacinone.

DOI： 10.1016/j.ecoenv.2022.113971

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Estimating susceptibility of rodenticide by molecular dynamics & machine learning using 3D structures of vitamin k epoxide reductase Invited Reviewed

Kazuki Takeda, Nobuaki Yasuo, Kanami Watanabe, Ryo Sato, Ryo Kamata, Masakazu Sekijima

The Pure and Applied Chemistry International Conference 2022 (PACCON2022) “Frontiers in Chemical Sciences for Health, Energy, and Sustainability” 971 - 975 2022.7

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (international conference proceedings)

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Development of a sandwich enzyme-linked immunosorbent assay (ELISA) to quantify γ-glutamyl-carboxylated clotting factor IX and assess redox susceptibility of anticoagulant chemicals Reviewed

Ryo SATO, Kanami WATANABE, Ryo KAMATA, Kazuki TAKEDA

Journal of Veterinary Medical Sciences 84 ( 6 ) 804 - 808 2022.6

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

Anticoagulant chemicals (ACCs) such as warfarin are widely used in medical applications as well as for their rodenticide properties. Their efficacy is greatly influenced by polymorphisms in the gene encoding vitamin K epoxide reductase (VKOR). Evaluation of the activity of ACCs toward VKOR variants is essential to determine their proper use. Presently, this is achieved by co-expressing VKOR of Rattus Norvegicus and human clotting factor IX in cultured cells and measuring inhibition of vitamin K-dependent gamma-glutamyl carboxylation of factor IX (glaFIX) activity. However, glaFIX has only been quantified using indirect methods like blood coagulation assays. We have developed a sandwich enzyme-linked immunosorbent assay using a glaFIX-specific antibody to quantify glaFIX and used this to analyze inhibition of VKOR activity by warfarin.

DOI： 10.1292/jvms.22-0079

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The effects of fipronil on emotional and cognitive behaviors in mammals Reviewed International journal

Tomohiro Suzuki, Anri Hirai, Kraisiri Khidkhan, Collins Nimako, Takahiro Ichise, Kazuki Takeda, Hazuki Mizukawa, Shouta M.M. Nakayama, Kei Nomiyama, Nobuhiko Hoshi, Mizuki Maeda, Tetsushi Hirano, Kazuyoshi Sasaoka, Noboru Sasaki, Mitsuyoshi Takiguchi, Mayumi Ishizuka, Yoshinori Ikenaka

Pesticide Biochemistry and Physiology 175 104847 - 104847 2021.4

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Fipronil is a phenylpyrazole insecticide that is widely used as a pesticide and a veterinary drug, although studies suggest that it could be toxic to mammals. The objectives of this study were to examine the pharmacokinetic profile of fipronil in mice, dogs, and cats, and to evaluate its effects on emotional and cognitive behaviors of dogs and cats using the data obtained from mice. The assessment of in vivo kinetics of fipronil was conducted in mice and dogs. We also performed behavioral tests (elevated plus-maze and Y-maze) and measured the levels of neurotransmitters in mice exposed to fipronil. In addition, the in vitro metabolism of fipronil were evaluated using liver microsomes of rats, mice, dogs, and cats. The results revealed that fipronil is distributed throughout the body (blood, brain, adipose tissue, and liver) of mice after dermal application. It was metabolized to fipronil sulfone primarily in the liver. The data on kinetics show that both fipronil and fipronil sulfone have a longer half-life in dogs and cats than in mice. The behavioral tests indicated that fipronil and fipronil sulfone could affect emotional and cognitive behaviors and alter the levels of neurotransmitters (dopamine in the striatum and serotonin in the hippocampus) in mice. Furthermore, we found that dogs and cats have a low ability to metabolize fipronil than mice and rats. However, further comprehensive studies are needed to determine whether fipronil affects the emotional and cognitive behaviors when administered to dogs and cats. To the best of our knowledge, this is the first study to examine the pharmacokinetic data and verify the effects of fipronil on emotional and cognitive behaviors of dogs and cats using the data obtained from mice.

DOI： 10.1016/j.pestbp.2021.104847

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Sensitivity of turtles to anticoagulant rodenticides: risk assessment for green sea turtles (Chelonia mydas) in the Ogasawara Islands and comparison of warfarin sensitivity among turtle species Reviewed International journal

Yoshiya Yamamura, Kazuki Takeda, Yusuke K. Kawai, Yoshinori Ikenaka, Chiyo Kitayama, Satomi Kondo, Chiho Kezuka, Mari Taniguchi, Mayumi Ishizuka, Shouta M.M. Nakayama

Aquatic Toxicology 233 105792 - 105792 2021.2

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Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Although anticoagulant rodenticides (ARs) are effectively used for the control of invasive rodents, nontarget species are also frequently exposed to ARs and secondary poisonings occur widely. However, little data is available on the effects of ARs, especially on marine organisms. To evaluate the effects of ARs on marine wildlife, we chose green sea turtles (Chelonia mydas), which are one of the most common marine organisms around the Ogasawara islands, as our primary study species. The sensitivity of these turtles to ARs was assessed using both in vivo and in vitro approaches. We administered 4 mg/kg of warfarin sodium either orally or intravenously to juvenile green sea turtles. The turtles exhibited slow pharmacokinetics, and prolongation of prothrombin time (PT) was observed only with intravenous warfarin administration. We also conducted an in vitro investigation using liver microsomes from green sea turtles, and two other turtle species (softshell turtle and red-eared slider) and rats. The cytochrome P450 metabolic activity in the liver of green sea turtles was lower than in rats. Additionally, vitamin K epoxide reductase (VKOR), which is the target enzyme of ARs, was inhibited by warfarin in the turtles at lower concentration levels than in rats. These data indicate that turtles may be more sensitive to ARs than rats. We expect that these findings will be helpful for sea turtle conservation following accidental AR-broadcast incidents.

DOI： 10.1016/j.aquatox.2021.105792

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The VKORC1 ER-luminal loop mutation (Leu76Pro) leads to a significant resistance to warfarin in black rats (Rattus rattus) Reviewed International journal

Kazuki Takeda, Yoshinori Ikenaka, Denis Fourches, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Dhoha Triki, Xinhao Li, Manabu Igarashi, Tsutomu Tanikawa, Mayumi Ishizuka

Pesticide Biochemistry and Physiology 173 104774 - 104774 2021.1

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

Well-known 4-hydroxycoumarin derivatives, such as warfarin, act as inhibitors of the vitamin K epoxide reductase (VKOR) and are used as anticoagulants. Mutations of the VKOR enzyme can lead to resistance to those compounds. This has been a problem in using them as medicine or rodenticide. Most of these mutations lie in the vicinity of potential warfarin-binding sites within the ER-luminal loop structure (Lys30, Phe55) and the transmembrane helix (Tyr138). However, a VKOR mutation found in Tokyo in warfarin-resistant rats does not follow that pattern (Leu76Pro), and its effect on VKOR function and structure remains unclear. We conducted both in vitro kinetic analyses and in silico docking studies to characterize the VKOR mutant. On the one hand, resistant rats (R-rats) showed a 37.5-fold increased IC50 value to warfarin when compared to susceptible rats (S-rats); on the other hand, R-rats showed a 16.5-fold lower basal VKOR activity (Vmax/Km). Docking calculations exhibited that the mutated VKOR of R-rats has a decreased affinity for warfarin. Molecular dynamics simulations further revealed that VKOR-associated warfarin was more exposed to solvents in R-rats and key interactions between Lys30, Phe55, and warfarin were less favored. This study concludes that a single mutation of VKOR at position 76 leads to a significant resistance to warfarin by modifying the types and numbers of intermolecular interactions between the two.

DOI： 10.1016/j.pestbp.2021.104774

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Comparison of two reducing agents dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro kinetic assay of vitamin K epoxide reductase. Reviewed International journal

Kazuki Takeda, Ayuko Morita, Yoshinori Ikenaka, Shouta M M Nakayama, Mayumi Ishizuka

Veterinary and animal science 9 100095 - 100095 2020.6

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Vitamin K epoxide reductase (VKOR) is a target enzyme for anticoagulants, such as warfarin, that are used as medicines or rodenticides. Assessing VKOR activity is required to ensure the proper usage of these drugs. Dithiothreitol (DTT) is a typical disulfide reductant that is used as a substrate for in vitro VKOR assays. However, DTT is considered problematic because of its side effects. Tris(3-hydroxypropyl)phosphine (THP) has been found to be a reliable alternative to DTT, as shown by kinetic analyses of the VKOR with them. THP showed significantly lower Vmax and Km values than those of DTT; however, there was no significant difference in their Vmax/Km and IC50 for warfarin.

DOI： 10.1016/j.vas.2020.100095

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Comparison of xenobiotic metabolism in phase I oxidation and phase II conjugation between rats and bird species. Reviewed International journal

Aksorn Saengtienchai, Yoshinori Ikenaka, Minami Kawata, Yusuke Kawai, Kazuki Takeda, Takamitsu Kondo, Nesta Bortey-Sam, Shouta M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 214 28 - 35 2018.12

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There have been many reports regarding toxic chemicals in birds. Chemicals are mainly metabolized in the liver through phase I oxidation by cytochrome P450 (CYP) and phase II conjugation by conjugated enzymes, such as UDP-glucuronosyltransferase (UGT), sulfotransferase (SULT), glutathione-S-transferase (GST), etc. Xenobiotic metabolism differs among bird species, but little detailed information is available. In the present study, the four-ring polycyclic aromatic hydrocarbon (PAH), pyrene, was used as a model xenobiotic to clarify the characteristics of xenobiotic metabolism in birds compared with laboratory animals by in vivo and in vitro studies. Plasma, bile, and excreta (urine and feces) were collected after oral administration of pyrene and analyzed to clarify xenobiotic metabolism ability in chickens and quails. Interestingly, pyrenediol-glucuronide sulfate (PYDOGS) and pyrenediol-diglucuronide (PYDOGG) were present in chickens and quails but not in rats. In addition, the area under the curve (AUC), maximum plasma concentration (Cmax), and time to maximum plasma concentration (Tmax) of pyrene-1-sulfate (PYOS) were higher than those of the parent molecule, pyrene, while the elimination half-life (t1/2) and mean residence time (MRT) were faster than those of the parent pyrene. With regard to sulfation of 1-hydroxypyrene (PYOH), the maximum velocity (Vmax) and Michaelis constant (Km) of rat liver cytosol were greater than those of chicken and quail liver cytosol. Furthermore, Vmax/Km of UGT activity in rat liver microsomes was also greater than those of chicken and quail liver microsomes. Characterization of xenobiotic metabolism revealed species differences between birds and mammals, raising concerns about exposure to various xenobiotics in the environment.

DOI： 10.1016/j.cbpc.2018.08.007

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Investigation of hepatic warfarin metabolism activity in rodenticide-resistant black rats (Rattus rattus) in Tokyo by in situ liver perfusion. Reviewed International journal

Kazuki Takeda, Yoshinori Ikenaka, Kazuyuki D Tanaka, Shouta M M Nakayama, Tsutomu Tanikawa, Hazuki Mizukawa, Mayumi Ishizuka

Pesticide biochemistry and physiology 148 42 - 49 2018.6

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Anti-blood coagulation rodenticides, such as warfarin, have been used all over the world. They inhibit vitamin K epoxide reductase (VKOR), which is necessary for producing several blood clotting factors. This inhibition by rodenticides results in lethal hemorrhage in rodents. However, heavy usage of these agents has led to the appearance of rodenticide-resistant rats. There are two major mechanisms underlying this resistance, i.e., mutation of the target enzyme of warfarin, VKOR, and enhanced metabolism of warfarin. However, there have been few studies regarding the hepatic metabolism of warfarin, which should be related to resistance. To investigate warfarin metabolism in resistant rats, in situ liver perfusion of warfarin was performed with resistant black rats (Rattus rattus) from Tokyo, Japan. Liver perfusion is an in situ methodology that can reveal hepatic function specifically with natural composition of the liver. The results indicated enhanced hepatic warfarin hydroxylation activity compared with sensitive black rats. On the other hand, in an in vitro microsomal warfarin metabolism assay to investigate kinetic parameters of cytochrome P450, which plays a major role in warfarin hydroxylation, the Vmax of resistant rats was slightly but significantly higher compared to the results obtained in the in situ study. These results indicated that another factor like electron donators may also contribute to the enhanced metabolism in addition to high expression of cytochrome P450.

DOI： 10.1016/j.pestbp.2018.03.018

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Novel revelation of warfarin resistant mechanism in roof rats (Rattus rattus) using pharmacokinetic/pharmacodynamic analysis. Reviewed International journal

Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D Tanaka, Shouta M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

Pesticide biochemistry and physiology 134 1 - 7 2016.11

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Roof rats (Rattus rattus) live mainly in human habitats. Heavy use of rodenticides, such as warfarin, has led to the development of drug resistance, making pest control difficult. There have been many reports regarding mutations of vitamin K epoxide reductase (VKOR), the target enzyme of warfarin, in resistant rats. However, it has been suggested there are other mechanisms of warfarin resistance. To confirm these possibilities, closed colonies of warfarin-susceptible roof rats (S) and resistant rats from Tokyo (R) were established, and the pharmacokinetics/pharmacodynamics of warfarin in rats from both colonies was investigated. R rats had low levels of warfarin in serum and high clearance activity. These rats can rapidly metabolize warfarin by hydroxylation. The levels of accumulation in the organs were lower than those of S rats. R rats administered warfarin showed high expression levels of CYP2B, 2C, and 3A, which play roles in warfarin hydroxylation, and may explain the high clearance ability of R rats. The mechanism of warfarin resistance in roof rats from Tokyo involved not only mutation of VKOR but also high clearance ability due to high levels of CYP2B, 2C and 3A expression possibly induced by warfarin.

DOI： 10.1016/j.pestbp.2016.04.004

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Books

Experimental Pharmacology and Toxicology, 4th ed. (written in Japanese)

Japanese Society of Veterinary Pharmacology, Toxicology（ Role： ContributorVIII-16: Skin toxicity test using cultured cells, IX-18: Micronucleus test using mice, IX-19: Chromosome aberration test using cultured mammalian cells, X-20: Teratogenicity test using chicken embryos,）

Bun-eido shuppan co. ltd. 2023.3 （ ISBN:9784830032868 ）

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Total pages：240 Language：Japanese Book type：Textbook, survey, introduction

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Essential Textbook for Pet Nurse（written in Japanese）

（ Role： Contributor）

Gakken 2022.12 （ ISBN:9784780914672 ）

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Total pages：800 Language：Japanese Book type：Textbook, survey, introduction

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Encyclopedia of Pests

Tsuda Yoshio, Agui Nobuaki, Tanigawa Tsutomu, Natsuaki Masaru, Hayashi Toshihiko, Hirabayashi Kimio, Yamauchi Takeo（ Role： Contributor1-69 Rodenticide resistance）

Asakura Publishing Co.,Ltd. 2020.5 （ ISBN:9784254640489 ）

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Total pages：viii, 429p, 図版 [8] p Language：Japanese

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MISC

Target Discovery through Structural Science: Comprehensive Molecular Docking to the AlphaFold-Predicted Structural Proteome Invited

Kazuki Takeda

Newsletter of the Japan Society for Endocrine Disruptors Research 28 ( 2 ) 12 - 12 2025.10

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Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Japan Society of Endocrine Disruptors Research

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Synthesis of vitamin K3 derivatives exhibiting 3CL protease inhibitory activity for antiviral drugs

笹嶋美紀, 早川真由, 小原沢諒人, 武田一貴, 廣田佳久, 須原義智

日本薬学会年会要旨集(Web) 145th 2025

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フェロトーシスを抑制するビタミンKの摂取によるマウス新奇物体認識への影響

加藤彩花, 渡邉莉菜, 川村悠, 加藤主税, 竹腰進, 高木基樹, 高木基樹, 須原義智, 須原義智, 廣田佳久, 廣田佳久, 武田一貴

日本分子生物学会年会プログラム・要旨集(Web) 48th 2025

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構造科学で紐解くビタミンKサイクル関連タンパク質の薬物感受性・種差・個体差

武田一貴

ビタミン 99 ( 3 ) 2025

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リトコール酸骨格を持つ新規殺鼠剤Dcha-20の作用機序解析

深松美咲, 岡村佳奈, 小倉千春, 長岡慧, 影近弘之, 棚谷綾, 武田一貴

医薬品毒性機序研究会要旨(Web) 7th 2025

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タンパク質構造予測と分子ドッキング計算を用いた芳香族炭化水素受容体へのリガンド結合親和性予測の検討

中村友香, 羽山哲平, 菅原琳, 鎌田亮, 武田一貴

医薬品毒性機序研究会要旨(Web) 7th 2025

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網羅的分子ドッキングシミュレーションによる化学物質結合タンパク質in silico評価法の構築

菅原琳, 羽山哲平, 鎌田亮, 武田一貴

医薬品毒性機序研究会要旨(Web) 7th 2025

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タンパク質立体構造と分子シミュレーションによる毒性評価

武田一貴

医薬品毒性機序研究会要旨(Web) 7th 2025

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ビタミンK₃が有する3CLプロテアーゼ阻害活性を増強させた誘導体の創製

笹嶋美紀, 岡本実佳, 小原沢諒人, 早川真由, 武田一貴, 廣田佳久, 馬場昌範, 須原義智

ビタミン 99 ( 3 ) 2025

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非セコステロイド型ビタミンD誘導体Dcha-20の殺鼠剤への応用

吉原実唯菜, 小倉千春, 岡村佳奈, 深松美咲, 鎌田亮, 武田一貴, 長岡慧, 影近弘之, 棚谷綾

ビタミン 99 ( 3 ) 2025

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リトコール酸誘導体Dcha-20の殺鼠活性

棚谷綾, 岡村佳奈, 小倉千春, 武田一貴, 長岡慧, 影近弘之

ビタミン 99 ( 2 ) 2025

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Selection of complex/simple in vitro evaluation models for efficient understanding and mitigation of toxicity

浜村えり, 白井勇司, 井口拓馬, 武田一貴, 藤本和則, 土屋由美

Journal of Toxicological Sciences 49 ( Supplement (CD-ROM) ) 2024.7

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DOI： 10.14869/toxpt.51.1.0_S35-5

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AI (deep learning) based toxicity prediction methods Invited Reviewed

Kazuki Takeda

Toxicology Topics Commentary, Japanese Society of Toxicology 2024.7

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Environmental Pollution Problem Tackled by Veterinary x Toxicity x Simulation x AI Invited

Kazuki Takeda

Technology Akros ( 125 ) 6 2024.6

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Protein Structure Prediction by Deep Learning for Environmental Toxicology Research Invited

Kazuki Takeda

Newsletter of the Japan Society of Endocrine Disruptors Research 27 ( 1 ) 5 2024.6

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3CLプロテアーゼ阻害活性を有する新規ビタミンK3誘導体の創製

笹嶋美紀, 小原沢諒人, 早川真由, 武田一貴, 廣田佳久, 須原義智

ビタミン 98 ( 4 ) 234 - 234 2024.4

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Language：Japanese Publisher：(公社)日本ビタミン学会

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COVID-19治療を志向したSARS-CoV-2増殖抑制効果を目指した新規ビタミンK誘導体の創製

早川真由, 本間大暉, 小原沢諒人, 武田一貴, 須原義智, 廣田佳久

日本生化学会大会(Web) 97th 2024

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Understanding species differences in drug susceptibility based on protein 3D structures: A case study on VKOR

武田一貴, 武田一貴, 宮前萌優, 羽山哲平, 山本早苗, 鎌田亮

Journal of Toxicological Sciences 49 ( Supplement (CD-ROM) ) 2024

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Evaluation of the Aromatic Hydrocarbon Receptor (AhR) Activation Potential by Sensitizer-Related Compounds in Waste Paper Recycling Effluent

SUN Jiayan, 武田一貴, 武田一貴, 寺崎正紀, 江口哲史, 久保田彰, 更田葵依, 清水敬太, 鎌田亮

Journal of Toxicological Sciences 49 ( Supplement (CD-ROM) ) 2024

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偏微分方程式モデリングを用いた野生動物における抗血液凝固系殺鼠剤体内動態の予測

深松美咲, 片山雅史, 中山翔太, 宮前萌優, 鎌田亮, 武田一貴

日本獣医学会学術集会講演要旨集 167th 2024

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Toward of vitamin K derivatives directed against inhibition of enzymes involved in SARS-CoV-2 proliferation

早川真由, 本間大暉, 小原沢諒人, 武田一貴, 須原義智, 廣田佳久

日本薬学会年会要旨集(Web) 144th 2024

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3CLプロテアーゼ阻害作用を有するビタミンK₃誘導体の探索

笹嶋美紀, 小原沢諒人, 早川真由, 武田一貴, 廣田佳久, 須原義智

日本薬学会関東支部大会講演要旨集(CD-ROM) 68th 2024

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リトコール酸誘導体Dcha-20の殺鼠剤としての有用性の検証

小倉千春, 岡村佳奈, 長岡慧, 武田一貴, 影近弘之, 棚谷綾

ビタミン 98 ( 4 ) 2024

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Structural biology using genomic information: exploring the post-AlphaFold2 era Invited Reviewed

武田一貴

地球環境 29 ( 2 ) 173 - 181 2024

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Improvement by 3D image analysis of the penetrating site and body position of captive bolt gun to properly stunning of cattle at a commercial slaughter facility

高橋史昭, 三浦弘, 前田洋佑, 武田一貴

食肉に関する助成研究調査成果報告書 42 2024

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パーフルオロアルキル化合物(PFASs)の日常摂取レベル濃度での9種混合曝露によるマウス精巣毒性影響の評価

吉村和加菜, 斉藤多喜, 佐々木さくら, 杉山真言, 鎌田亮, 武田一貴

日本獣医学会学術集会講演要旨集 167th 2024

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Pharmacokinetic and Pharmacodynamic (PK/PD) Analysis to Assess Sensitivity of Wild Roof Rats (Rattus rattus) in the Bonin Islands to Rodenticide Diphacinone

田中康太郎, 清水敬太, 佐藤美侑, 片山雅史, 中山翔太, 近藤理美, 池中良徳, 一瀬貴大, 石塚真由美, 鎌田亮, 小山田佑輔, 港隆一, 橋本琢磨, 田中和之, 木村悟朗, 谷川力, 加藤恵介, 日下部太一, 武田一貴

環境化学討論会要旨集(CD-ROM) 32nd 2024

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Evaluation of species differences in susceptibility to anticoagulant rodenticides based on in silico protein structure analysis.

宮前萌優, 山本早苗, 鎌田亮, 武田一貴

環境化学討論会要旨集(CD-ROM) 32nd 2024

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構造生物学に基づくビタミンと殺鼠剤の新展開

武田一貴

ビタミン 98 ( 9 ) 2024

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COVID-19の治療を目指した3CL ProteaseおよびRdRp阻害作用を有するビタミンK誘導体の創出

早川真由, 小原沢諒人, 本間大暉, 武田一貴, 須原義智, 廣田佳久

日本分子生物学会年会プログラム・要旨集(Web) 47th 2024

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AlphaFold2全プロテオーム予測構造に対する網羅的分子ドッキングによる「ドッキングプロテオミクス解析」の基礎的構築

菅原琳, 羽山哲平, 鎌田亮, 武田一貴

日本分子生物学会年会プログラム・要旨集(Web) 47th 2024

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Pharmacokinetics of Hybrid Vitamin K Derivatives Incorporating Retinoic Acid Structure Oriented for the Treatment of Neurodegenerative Diseases

廣田佳久, 渡邉莉菜, 武田一貴, 須原義智

衛生薬学・環境トキシコロジー講演要旨集 2024 2024

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AlphaFold2全構造プロテオームを用いたBinding-Proteomics評価系の構築

羽山哲平, 菅原琳, 鎌田亮, 武田一貴

日本獣医学会学術集会講演要旨集 167th 2024

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~From Fieldwork to AI ~ Veterinary Environmental Toxicology by Anything Goes Invited

Kazuki Takeda

Promotion of Veterinary Science ( 11 ) 28 - 30 2023.3

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Effect of diphacinone on immortalized cells from green sea turtle (Chelonia mydas)

片山雅史, 福田智一, 武田一貴, 近藤理美, 清野透, 中山翔太, 中山翔太

日本地球惑星科学連合大会予稿集(Web) 2023 2023

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Evolutionary history of UGT genes involved in xenobiotic metabolism in mammals and birds and their relationship to plants

川合佑典, 池中良徳, 池中良徳, 生城真一, 西川美宇, 一色乃亜, 武田一貴, 中山翔太, 中山翔太, 久保田彰, 石塚真由美

日本進化学会大会プログラム・講演要旨集(Web) 25th 2023

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Evaluation of Rodenticide Susceptibility of the Natural Monument; Bonin Flying Fox using AlphaFold2 and Molecular Simulation

武田一貴, 武田一貴, 川合佑典, 安尾信明, 関嶋政和, 真砂皓大, 池中良徳, 堀越和夫, 鈴木創, 石塚真由美, 中山翔太

環境化学討論会要旨集(CD-ROM) 31st 2023

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Exploratory Research of Toxicity Assessment Using Protein Structure Prediction by AlphaFold2 Invited

Kazuki Takeda

Journal of Toxicological Sciences 48 ( Supplement (CD-ROM) ) 2023

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ゾウFANCL-FANCB間の相互作用はヒトホモログより強い

日置七海, 北野泰佑, 朱子達, 武田一貴, 折野宏一, 吉川泰永

日本獣医学会学術集会講演要旨集 166th 2023

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Exploration of In Vivo Factors Contributing to Differences in Pharmacokinetics between cis-trans Isomers of Antihemostatic Rodenticides

Moyu Miyamae, Satoru Nagaoka, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

Journal of Toxicological Sciences 48 ( Supplement (CD-ROM) ) 2023

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PACCON2022 Presentation Log: Thailand in Illusion Invited

Kazuki Takeda

Journal of environmental chemistry 32 ( 3 ) 36 - 38 2022.9

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Establishment of bioresources for evaluation of environmental pollutants in the green sea turtle

片山雅史, 福田智一, 大沼学, 武田一貴, 近藤理美, 中山翔太, 中山翔太

日本地球惑星科学連合大会予稿集(Web) 2022 2022

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Construction of a method for predicting animal species differences in chemical sensitivity from human single nucleotide polymorphisms using molecular simulation

TAKEDA Kazuki, YASUO Nobuaki, SEKIJIMA Masakazu

Annual Meeting of the Japanese Society of Toxicology 49.1 S29-3 2022

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Language：Japanese Publisher：The Japanese Society of Toxicology

Chemicals in the environment can cause poisoning and death in wildlife. For this reason, environmental safety assessments of chemicals have been required worldwide in recent years. It is known that there are large species differences in sensitivity to chemicals, and toxic effects/lethal doses can be several hundred times greater in some species than in others. One of the reasons for this is the difference in binding ability to chemical substances caused by genetic polymorphisms of target molecules/drug-metabolizing enzymes. For safety evaluation in wild animals, it is difficult to extrapolate the results of animal experiments for the above reasons, and in vivo studies in higher wild animals such as mammals and birds are contraindicated. For these reasons, there is a need to develop a method for predicting chemical sensitivity in wild animals.

In this study, warfarin, an anticoagulant chemical, was used as a model compound. It binds to the active center of vitamin K epoxide reductase (VKOR) and inhibits the production of vitamin K-dependent coagulation factors. As a drug, it is problematic because of individual differences in therapeutic range due to VKOR gene polymorphisms in humans and resistance due to VKOR mutations in wild rodents. In addition, many poisoning deaths in wild animals have been reported in Europe and the United States from second-generation antihemolytic rodenticides with enhanced medicinal effects. In order to use rodenticides appropriately, it is necessary to evaluate the susceptibility of wild animals in areas where they are sprayed.

Therefore, we attempted to construct a machine learning model to predict VKOR IC50 values in wild animals based on the 50% inhibition of activity (IC50) values of human and rodent VKOR gene polymorphisms for which relatively large amounts of measured data are available. The steric structures of these VKOR variants were constructed using Alphafold2, and the binding states with rodenticides were calculated using molecular docking and molecular dynamics. IC50 values were predicted from multivariate parameters of molecular dynamics over time. As an evaluation method for wild animals for which measured values are almost unavailable, a semi-supervised method was employed to learn by clustering with gene polymorphisms that have high similarity to the above and have measured values.

DOI： 10.14869/toxpt.49.1.0_s29-3

CiNii Research

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Characterization of clotting factor IX splicing variant 2 by cultured cell method and AlphaFold2

WATANABE Kanami, SATO Ryo, KAMATA Ryo, TAKEDA Kazuki

Annual Meeting of the Japanese Society of Toxicology 49.1 P-17E 2022

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Clotting factor Ⅸ (FⅨ) activates coagulation by undergoing vitamin K-dependent conversion of a glutamic acid residue to γ-carboxyglutamic acid (Gla). Splicing variant1 (V1) and variants2 (V2) are registered in NCBI as FⅨ. Both are thought to show coagulation activity, but little is known in coagulation inhibition by warfarin in V2. In this study, we analyzed V2, by measuring coagulation ability and warfarin sensitivity in vitro using cultured cells. We also analyzed the complex with factors Ⅶ and Ⅺ by in silico structure estimation using AlphaFold2.

In the coagulation activity test, V1 showed coagulation activity but V2 did not. Next, in the determination of GlaFⅨ by sandwich ELISA, Gla conversion was inhibited by warfarin in both V1 and V2. Furthermore, quantification of GlaFⅨ and totalFⅨ by Western blot suggested that V2 undergoes Gla conversion. Lastly, the result of in silico structure estimation showed that V2 and factorⅦ did not form a complex, but V2 and factorⅪ bound at a specific domain.

This study shows that V2 doesn’t possess blood coagulation ability when activated by factorⅧ and factorⅪ, but undergoes Gla conversion. In addition, AlphaFold2 showed image in which V2 and FactorⅨ were partially combined.

As a conclusion, V2 may act as a competitive inhibitor of V1 and may be a regulator in blood coagulation activity. In fact, the result of RT-PCR in mouse liver showed that V2 was quantified by about 1.5% compared to V1. Further researches are needed to confirm more accurate quantification and expression in tissue samples and to determine whether V2 acts as a competitive inhibitor against V1.

DOI： 10.14869/toxpt.49.1.0_p-17e

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惹起相を有する皮膚感作性試験開発のためのマウスにおける感作性物質曝露と感作反応の検証

鎌田亮, 中村和市, 武田一貴

日本獣医学会学術集会講演要旨集 164th (CD-ROM) 2021

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Pharmacokinetics/dynamics analysis of anticoagulant rodenticides with Egyptian fruit bats (Rousettus aegyptiacus)

TAKEDA Kazuki, MANAGO Kosuke, NAKAYAMA Shouta M.M., KAWAI Yusuke K., IKENAKA Yoshinori, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 47.1 ( Supplement ) P-244 - 244 2020

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[Introduction]Anticoagulant rodenticides (ARs) have been used for control of rodents' population. ARs inhibit vitamin K epoxide reductase (VKOR) playing role in producing clotting factors, which results in lethal hemorrhage in rodents. However, ARs-spread have caused accidental death to non-target species. Thus, it is necessary for proper usage of ARs to evaluate sensitivity of wild species. In Japan, ARs were used in Bonin (Ogasawara) islands. Bonin fruit bat (Pteropus pselaphon) is the only endemic mammalian species in the islands, however, ARs-sensitivity of fruit bats has been unclear. For the assessment, we performed pharmacokinetics analysis of ARs with Egyptian fruit bats (Rousettus aegyptiacus).[Materials & Methods]Animals; four female adult egyptian fruit (EF) bats and female Sprague Dawley (SD) rats (10 weeks old) were used in the study. Chemicals; we used two ARs (warfarin and diphacinone). Both of them were classified in the first generation ARs and have been commonly used in Japan. Methods; 4 mg/kg of warfarin sodium disloved in saline water was orally administrated to the EF bats and SD rats. Blood was taken from wing vein of EF bats and tail vein of SD rats. Prothrombin time (PT) was measured by CoaguCheck XS (Roche). Warfarin and its metabolites was extracted by liquid-liquid distribution with ether and their concentration was measured by HPLC-MS/MS (Shimadzu). After two month of warfarin administration, 4 mg/kg of diphacinone disolved in corn oil was also administrated to EF bats.[Results and Discussion]After warfarin administration, prolonged PT was observed in both EF bats and SD rats, however, EF bats recovered to normal value earlier than SD rats. EF bats showed higher clearance of warfarin than SD rats and produced higher amounts of warfarin-metabolites than SD rats. These results suggested EF bats were less sensitive to warfarin than SD rats due to their high warfarin excretion ability. On the other hand, PT prolonged earlier in EF bats after diphacinone exposure, although plasma concentration of diphacinone was significantly lower in EF bats than in SD rats. These results indicated fruit bats showed different sensitivity to warfarin and diphacinone, although they have same mode of action.[Conclusion]This study revealed Egyptian fruit bats were relatively tolerant to warfarin but susceptible to diphacinone compared to rats. Further study with Bonin fruit bats is required for safety usage of ARs.

DOI： 10.14869/toxpt.47.1.0_p-244

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東京都小笠原諸島における殺鼠剤散布のアオウミガメへのリスク評価

山村快哉, 武田一貴, 中山翔太, 川合佑典, 森田鮎子, 池中良徳, 小松謙之, 北山知代, 近藤理美, 石塚真由美

日本野生動物医学会大会・講演要旨集 25th 2019

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In Vivo Pharmacokinetic/pharmacodynamic analysis of first-generation anticoagulant rodenticide warfarin with Egyptian fruit bats (Rousettus aegyptiacus) for risk assessment of Bonin fruit bats (Pteropus pselaphon)

NAKAYAMA Shouta M.M., MANAGO Kosuke, TAKEDA Kazuki, MORITA Ayuko, IKENAKA Yoshinori, ISHIZUKA Mayumi

環境化学討論会要旨集(CD-ROM) 28th 2019

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DDTによる異物代謝酵素の遺伝子発現撹乱と健康影響評価

本平航大, 池中良徳, 池中良徳, BEYENE Yared, 中山翔太, 江口哲史, 武田一貴, WEPENER Victor, SMIT Nico, VAN VUREN Johan, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

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「基質結合部位からも活性残基からも離れたアミノ酸変異はどのように殺鼠剤抵抗性に寄与するのか?」ドッキングシミュレーション・分子動力学シミュレーションによるビタミンKエポキシド還元酵素の解析

武田一貴, 池中良徳, 池中良徳, FOURCHES Denis, 田中和之, 中山翔太, 谷川力, TRIKI Dhoha, LI Xinhao, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 42nd 2019

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What Makes Super Rats "Super"? ~Investigation of Mechanisms of Rodenticide Resistance~

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D., NAKAYAMA Shouta M.M., TANIKAWA Tsutomu, MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 43 ( Supplement ) P - 71 2018

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[in Japanese] 

DOI： 10.14869/toxpt.45.1.0_P-71

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スーパーラットは何が「スーパー」?東京の殺鼠剤抵抗性ラットにおける抵抗性獲得機序の解析

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 41st 2018

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HPLC-APCI-MSを用いたビタミンKの網羅的検出とin vitroビタミンKエポキシド還元酵素活性の評価

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 27th 2018

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殺鼠剤に弱い野生動物種を探る:標的分子ビタミンKエポキシド還元酵素(VKOR)の鳥類における特徴および種差

中山翔太, 森田鮎子, 池中良徳, 石井千尋, 水川葉月, 武田一貴, 川合佑典, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 石塚真由美

日本野生動物医学会大会・講演要旨集 24th 2018

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東京のワルファリン抵抗性ラットの抵抗性獲得機序の解明:ペントースリン酸経路の亢進を通したワルファリン代謝の活性化

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本生化学会大会(Web) 90th 2017

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東京由来殺鼠剤抵抗性ラットを用いた新規抵抗性獲得機序の解明:ペントースリン酸経路の亢進による殺鼠剤代謝能の活性化

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本獣医学会学術集会講演要旨集 160th 2017

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Super rat (Rodenticide Resistant Rats) is super metabolizer of warfarin ~revealed by in situ, in vitro approach~

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D, NAKAYAMA Shouta M.M, TANIKAWA Tsutomu, MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 42 ( Supplement ) P - 21 2017

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DOI： 10.14869/toxpt.44.1.0_P-21

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In situ肝灌流試験によるワルファリン抵抗性クマネズミでのワルファリン代謝能の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 26th 2017

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Investigation of pharmacokinetics / pharmacodynamics status in a closed colony of warfarin resistant rats (Rattus rattus) from Tokyo

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D., TANIKAWA Tsutomu, NAKAYAMA Shouta M.M., MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 41 ( Supplement ) P - 89 2016

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DOI： 10.14869/toxpt.43.1.0_P-89

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In situ肝灌流モデルによる殺鼠剤抵抗性野生クマネズミにおけるワルファリン代謝能の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 39th 2016

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野生齧歯類におけるワルファリン抵抗性獲得機序の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 24th 2015

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Presentations

Binding-Proteomics in the AlphaFold Era: Scalable Proteome-Wide Docking for Mechanistic Toxicology and Cross-Species Risk Assessment. Invited

Kazuki TAKEDA

SOT 65th Annual Meeting 2026.3

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Event date： 2026.3

Language：English Presentation type：Oral presentation (invited, special)

Global Session: Structure-Based Toxicology in the AlphaFold Era:
Emerging Opportunities and Unmet Challenges

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Development of Lithocholic Acid Derivatives with High Vitamin D Activity and Their Application to Rodenticides

Miyuna Yoshihara, Chiharu Ogura, Kana Okamura, Misaki Fukamatsu, Ryo Kamata, Kazuki Takeda, Satoru Nagaoka, Hiroyuki Kagechika, Aya Tanatani

The 36th Annual Meeting of the Japanese Society for Retinoid Research 2025.10

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Event date： 2025.10

Language：Japanese Presentation type：Oral presentation (general)

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網羅的分⼦ドッキングシミュレーションによる化学物質結合タンパク質in silico評価法の構築【優秀発表賞】

菅原琳, 羽山哲平, 鎌田亮, 武田一貴

第7回医薬品毒性機序研究会

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Event date： 2025.1

Presentation type：Poster presentation

優秀発表賞受賞

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リトコール酸⾻格を持つ新規殺⿏剤Dcha-20の作⽤機序解析

深松美咲, 岡村佳奈, 小倉千春, 岡慧, 影近弘之, 棚谷綾, 武田一貴

第7回医薬品毒性機序研究会

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Event date： 2025.1

Presentation type：Poster presentation

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タンパク質構造予測と分⼦ドッキング計算を⽤いた芳⾹族炭化⽔素受容体へのリガンド結合親和性予測の検討

中村友香, 羽山哲平, 菅原琳, 鎌田亮, 武田一貴

第7回医薬品毒性機序研究会

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Event date： 2025.1

Presentation type：Poster presentation

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タンパク質⽴体構造と分⼦シミュレーションによる毒性評価 Invited

武田一貴

第7回医薬品毒性機序研究会 2025.1

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Event date： 2025.1

Presentation type：Symposium, workshop panel (nominated)

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付加体毒性評価における分子シミュレーション Invited

武田一貴

日本毒性学会付加体科学部会第2回シンポジウム 2024.10

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Event date： 2024.10

Presentation type：Symposium, workshop panel (nominated)

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Predicting the Pharmacokinetics of Anticoagulant Rodenticides in Wildlife Using Partial Differential Equation Modeling

Misaki Fukamatsu, Masafumi Katayama, Shouta M.M. Nakayama, Moyu Miyamae, Ryo Kamata, Kazuki Takeda

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Event date： 2024.9

Presentation type：Oral presentation (general)

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Assessment of Testicular Toxicity of Mixed Exposure of Nine Perfluoroalkyl Substances (PFASs) at Concentrations Relevant to Daily Intake

Wakana Yoshimura, Taki Saito, Sakura Sasaki, Makoto Sugiyama, Ryo Kamata, Kazuki Takeda

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Event date： 2024.9

Presentation type：Oral presentation (general)

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In silico Binding-Proteomics Evaluation Pipeline Using AlphaFold2 Whole Structural Proteome

Teppei Hayama, Rin Sugawara, Ryo Kamata, Kazuki Takeda

The 167th Annual Meeting of The Society of Japanese Veterinary Medical Sciences 2024.9

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Event date： 2024.9

Presentation type：Oral presentation (general)

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Comparisons of dominant species of rodents and their resistance factors to anticoagulant rodenticides between Japan, the United States and Europe. Invited

Satoru Nagaoka, Yasushi Kiyokawa, Tsutomu Tanikawa, Kazuyuki Tanaka, Kazuki Takeda, Mayumi Ishizuka

XXVII International Congress of Entomology (ICE2024) 2024.8

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Event date： 2024.8

Language：English Presentation type：Oral presentation (invited, special)

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Evaluation of the Aromatic Hydrocarbon Receptor (AhR) Activation Potential by Sensitizer-Related Compounds in Waste Paper Recycling Effluent

Jiayan Sun, Kazuki Takeda, Masanori Terasaki, Akifumi Eguchi, Akira Kubota, Aoi Sarata, Keita Shimizu, Ryo Kamata

The 51st Annual Meeting of Japanese Society of Toxicology 2024.7

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Event date： 2024.7

Language：English Presentation type：Poster presentation

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Machine Learning Analysis of the Results of the Japanese Society of Toxicology Career Path Questionnaire Invited

Kazuki Takeda

The 51st Annual Meeting of Japanese Society of Toxicology 2024.7

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Event date： 2024.7

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Understanding species differences in drug susceptibility based on protein 3D structures: A case study on VKOR

Kazuki Takeda, Moyu Miyamae, Teppei Hayama, Sanae Yamamoto, Ryo Kamata

The 51st Annual Meeting of Japanese Society of Toxicology 2024.7

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Event date： 2024.7

Language：Japanese Presentation type：Poster presentation

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小笠原父島の外来クマネズミにおける殺鼠剤ダイファシノンの薬物動態・薬力学（PK/PD）解析による感受性評価【SETAC賞】

田中康太郎, 清水敬太, 佐藤美侑, 片山雅史, 中山翔太, 近藤理美, 池中良徳, 一瀬貴大, 石塚真由美, 鎌田, 小山田佑輔, 港隆一, 橋本琢磨, 田中和之, 木村悟朗, 谷川力, 加藤恵介, 日下部太一, 武田一貴

第3回環境化学物質合同大会 2024.7

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Event date： 2024.7

Language：Japanese Presentation type：Poster presentation

SETAC賞受賞

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Evaluation of species differences in animal susceptibility to warfarin rodenticides based on in silico protein structure analysis

Moyu Miyamae, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

3rd Joint Conference on Environmental Chemicals 2024.7

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Event date： 2024.7

Language：Japanese Presentation type：Poster presentation

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Evaluation of Vitamin K Derivatives Directed at Inhibition of Enzymes Involved in SARS-CoV-2 Proliferation

Mayu Hayakawa, Daiki Homma, Ryoto Obarasawa, Kazuki Takeda, Yoshitomo Suhara, Yoshihisa Hirota

The 144th Annual Meeting of the Pharmaceutical Society of Japan

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Event date： 2024.3

Language：Japanese Presentation type：Oral presentation (general)

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Evaluation of sensitivity differences of anticoagulant rodenticide, diphacinone in four rodent strains by PK/PD analysis and PBPK modeling. Invited

Kazuki Takeda

GastroPlus® Users Meeting 2023 2023.10 Northern Science Consulting Inc.

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Event date： 2023.10

Language：Japanese Presentation type：Oral presentation (invited, special)

Venue：Webinar

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Environmentally harmful paper recycling? sensitizer compounds in paper recycling effluent, exerts dioxin-like effects on zebrafish (Danio rerio) via aryl hydrocarbon receptors International conference

Kazuki Takeda, Aoi Sarata, Masanori Terasaki, Akira Kubota, Keita Shimizu, Ryo Kamata

The international joint meeting of the 23rd International Conference on Cytochrome P450 (ICCP450) and the 38th Annual Meeting of the Japanese Society for the Study of Xenobiotics (JSSX) (2023 ICCP450/JSSX International Joint meeting) 2023.9

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Event date： 2023.9

Language：English Presentation type：Poster presentation

Venue：Shizuoka, Japan

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Evolutionary history of UGT genes involved in xenobiotic metabolism in mammals and birds and their relationship to plants

2023.9

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Event date： 2023.8 - 2023.9

Language：Japanese Presentation type：Oral presentation (general)

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Exploratory Research of Toxicity Assessment Using Protein Structure Prediction by AlphaFold2 Invited

Kazuki Takeda

The 50th Annual Meeting of the Japanese Society of Toxicology 2023.6

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Event date： 2023.6

Language：English Presentation type：Oral presentation (invited, special)

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Exploration of In Vivo Factors Contributing to Differences in Pharmacokinetics between cis-trans Isomers of Anti-Coagulant Rodenticides

Moyu Miyamae, Satoru Nagaoka, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

The 50h Annual Meeting of the Japanese Society of Toxicology 2023.6

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Event date： 2023.6

Language：English

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Evaluation of Rodenticide Susceptibility of the Natural Monument; Bonin Flying Fox using AlphaFold2 and Molecular Simulation

Kazuki Takeda, Yusuke K Kawai, Nobuaki Yasuo, Masakazu Sekijima, Kodai Manago, Yoshinori Ikenaka, Kazuo Horikoshi, Hajime Suzuki, Mayumi Ishizuka, Shouta MM Nakayama

2nd Joint Conference on Environmental Chemicals 2023.5

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Event date： 2023.5 - 2023.6

Language：Japanese Presentation type：Oral presentation (general)

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Application of cultured cells obtained from green sea turtles for evaluation of toxicity test with anticoagulant rodenticides.

Masafumi Katayama, Tomoichi Fukuda, Kazuki Takeda, Satomi Kondo, Toru Kiyono, Shouta Nakayama

Japanese Geoscience Union Meeting 2023

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Event date： 2023.5

Language：Japanese Presentation type：Oral presentation (general)

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Evaluation of Subchronic Toxicological Effects of Low Concentration Mixed Exposure of Nine Linear PFAS on Mice

Kazuki Takeda, Taki Saito, Sakura Sasaki, Akifumi Eguchi, Ryo Kamata

6th International Chemical Hazard Symposium 2023.1

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Event date： 2023.1

Language：English Presentation type：Oral presentation (general)

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Highly Accurate Protein Structure Prediction Using AlphaFold2 for Toxicity Assessment ～Environmental Pollutants as an Application Case Study～

Kazuki Takeda

The 5th Study Group on Toxicological Mechanisms of Pharmaceuticals 2022.12

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Event date： 2022.12

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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2022 年に捕獲した小笠原諸島父島の外来クマネズミにおける殺鼠剤ダイファシノンの薬物動態‧薬力学（PK/PD）解析による感受性評価【最優秀賞（口頭発表部門）】

清水敬太, 佐藤美侑, 片山雅史, 中山翔太, 池中良徳, 一瀬貴大, 石塚真由美, 鎌田亮, 小山田佑輔, 港隆一, 橋本琢磨, 田中和之, 谷川力, 武田一貴

第28回日本野生動物医学会大会 2022.9

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Event date： 2022.9

Language：Japanese Presentation type：Oral presentation (general)

最優秀賞（清水敬太、口頭発表部門）

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インク由来化学物質群のゼブラフィッシュ胚への芳香族炭化水素受容体(AhR)を介する毒性影響評価

更田葵依, 武田一貴, 清水敬太, 寺崎正紀, 鎌田亮

第165回日本獣医学会学術集会 2022.9

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Event date： 2022.9

Language：Japanese Presentation type：Oral presentation (general)

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小笠原諸島の外来クマネズミに対する抗血液凝固系殺鼠剤ダイファシノンの薬物動態・薬力学(PK/PD)解析による感受性評価【野生動物学分科会優秀発表賞】

清水敬太, 佐藤美侑, 片山雅史, 中山翔太, 池中良徳, 一瀬貴大, 石塚真由美, 鎌田亮, 小山田佑輔, 港隆一, 橋本琢磨, 武田一貴

第165回日本獣医学会学術集会 2022.9

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Event date： 2022.9

Language：Japanese Presentation type：Oral presentation (general)

野生動物学分科会優秀発表賞受賞（清水敬太）

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Characterization of Clotting Factor IX Transcript variant 2 by cultured cells assay and Alphafold2

Kanami Watanabe, Ryo Sato, Ryo Kamata, Kazuki Takeda

The 49th Annual Meeting of the Japanese Society of Toxicology 2022.6

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Event date： 2022.6 - 2022.7

Language：Japanese Presentation type：Oral presentation (general)

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Construction of a method for predicting animal species differences in chemical sensitivity from human single nucleotide polymorphisms using molecular simulation Invited

Kazuki Takeda, Nobuaki Yasuo, Masakazu Sekijima

The 49th Annual Meeting of the Japanese Society of Toxicology 2022.7

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Event date： 2022.6 - 2022.7

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Gazing at the risk of anticoagulant rodenticides (ARs) application from aquatic biota: Attempt to evaluate ARs toxicity in sea turtles

Yoshiya Yamamura, Satomi Kondo, Chiyo Kitayama, Kazuki Takeda, Takahiro Ichise, Yoshinori Ikenaka, Mayumi Ishizuka, Shota M.M. Nakayama

The 49th Annual Meeting of the Japanese Society of Toxicology 2022.7

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Event date： 2022.6 - 2022.7

Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Evaluation of Toxicological Effects of Low Concentration Mixed Exposure of Perfluoroalkyl Substances (PFASs) on Mice

Kazuki Takeda, Taki Saito, Sakura Sasaki, Akifumi Eguchi, Ryo Kamata

Joint Conference on Environmental Chemicals 2022.6

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Event date： 2022.6

Language：Japanese Presentation type：Oral presentation (general)

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Evaluation of Aromatic Hydrocarbon Receptor (AhR) Agonistic Activity of Sensitizer-Related Compounds Using Zebrafish

Aoi Sarata, Kazuki Takeda, Keita Shimizu, Masanori Terasaki, Ryo Kamata

Joint Conference on Environmental Chemicals 2022.6

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Event date： 2022.6

Language：Japanese Presentation type：Oral presentation (general)

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ウミガメにおける抗凝血殺鼠剤の感受性評価

中山翔太, 山村快哉, 近藤理美, 北山知代, 武田一貴, 一瀬貴大, 池中良徳, 石塚真由美

環境化学物質3学会合同大会 2022.6

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Event date： 2022.6

Language：Japanese Presentation type：Oral presentation (general)

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Development of resources for assessing the effects of pollutants on green sea turtles

Masafumi Katayama, Tomokazu Fukuda, Manabu Onuma, Kazuki Takeda, Satomi Kondo, Shouta MM Nakayama

Japan Geoscience Union Meeting 2022 2022.5

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Event date： 2022.5

Language：Japanese

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Characterization of Clotting Factor IX Transcript variant 2

Kanami Watanabe, Ryo Sato, Ryo Kamata, Kazuki Takeda

The 4th Spring Meeting of Japanese Society of Comparative Pharmacology & Toxicology 2022.2

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Event date： 2022.2

Language：Japanese Presentation type：Oral presentation (general)

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Characterization of Clotting Factor IX Transcript variant 2

Kanami Watanabe, Ryo Sato, Ryo Kamata, Kazuki Takeda

5th International Chemical Hazard Symposium 2022.2

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Event date： 2022.2

Presentation type：Oral presentation (general)

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Anticoagulant rodenticide in Bonin Islands, Japan "Galápagos of the Orient"-investigation of rodenticide-susceptibility of endemic species by toxicokinetic analysis Invited

Kazuki Takeda, Shouta Nakayama, Kosuke Manago, Yoshiya Yamamura, Yusuke Kawai, Yoshinori Ikenaka, Noriyuki Komatsu, Chiyo Kitayama, Satomi Kondo, Takuma Hashimoto, Ryuichi Minato, Yusuke Oyamada, Kazuo Horikoshi, Mayumi Ishizuka

Pacifichem 2021 (The 2021 International Chemical Congress of Pacific Basin Societies) 2021.12

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Event date： 2021.12

Language：English Presentation type：Symposium, workshop panel (nominated)

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A potential novel biomarker for kidney injury in chicken as an avian model

Chihiro Ishii, Yusuke K. Kawai, Yoshinori Ikenaka, Naoya Maekawa, Osamu Ichii, Shouta M.M. Nakayama, Kazuki Takeda, Mayumi Ishizuka

The 14th Asian Society of Conservation Medicine/ 27th Japanese Society of Zoo and Wildlife Medicine 2021 Joint Conference 2021.9

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Event date： 2021.9

Language：English Presentation type：Poster presentation

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Estimating susceptibility of rodenticide by molecular dynamics & machine learning using 3D structure of vitamin K epoxide reductase

Kazuki Takeda, Nobuaki Yasuo, Kanami Watanabe, Ryo Sato, Ryo Kamata, Masakazu Sekijima

29th Symposium on Environmental Chemistry 2021.6

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Event date： 2021.6

Language：Japanese Presentation type：Oral presentation (general)

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Estimating the impacts of mutations on warfarin resistance of vitamin K epoxide reductase based on cheminformatics approaches

Kazuki Takeda

4th Chemical Hazard Symposium & Symposium of Japan Society for Environmental Chemistry 2021.3

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Event date： 2021.3

Language：English Presentation type：Oral presentation (general)

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Comparison of Warfarin Sensitivity Among Turtle Species Focused on Green Sea Turtles (Chelonia mydas) in the Ogasawara Islands.

Yoshiya Yamamura, Kazuki Takeda, Yusuke K. Kawai, Yoshinori Ikenaka, Chiyo Kitayama, Satomi Kondo, Chiho Kezuka, Mari Taniguchi, Mayumi Ishizuka, Shouta M.M. Nakayama

SETAC North America 41st Annual Meeting

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Event date： 2020.11

Language：English Presentation type：Oral presentation (general)

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Pharmacokinetic/Pharmacodynamic Analysis of Anticoagulant Rodenticides With Egyptian Fruit Bats (Pteropus pselaphon)

Kazuki Takeda, Kosuke Manago, Shouta M.M. Nakayama, Yusuke K. Kawai, Yoshinori Ikenaka, Mayumi Ishizuka

SETAC North America 41st Annual Meeting SETAC SciCon2

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Event date： 2020.11

Language：English Presentation type：Oral presentation (general)

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Pharmacokinetics/dynamics analysis of anticoagulant rodenticides with Egyptian fruit bats (Rousettus aegyptiacus)

TAKEDA Kazuki, MANAGO Kosuke, NAKAYAMA Shouta M.M., KAWAI Yusuke K., IKENAKA Yoshinori, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 2020 The Japanese Society of Toxicology

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Event date： 2020

Language：Japanese

[Introduction]Anticoagulant rodenticides (ARs) have been used for control of rodents' population. ARs inhibit vitamin K epoxide reductase (VKOR) playing role in producing clotting factors, which results in lethal hemorrhage in rodents. However, ARs-spread have caused accidental death to non-target species. Thus, it is necessary for proper usage of ARs to evaluate sensitivity of wild species. In Japan, ARs were used in Bonin (Ogasawara) islands. Bonin fruit bat (Pteropus pselaphon) is the only endemic mammalian species in the islands, however, ARs-sensitivity of fruit bats has been unclear. For the assessment, we performed pharmacokinetics analysis of ARs with Egyptian fruit bats (Rousettus aegyptiacus).[Materials & Methods]Animals; four female adult egyptian fruit (EF) bats and female Sprague Dawley (SD) rats (10 weeks old) were used in the study. Chemicals; we used two ARs (warfarin and diphacinone). Both of them were classified in the first generation ARs and have been commonly used in Japan. Methods; 4 mg/kg of warfarin sodium disloved in saline water was orally administrated to the EF bats and SD rats. Blood was taken from wing vein of EF bats and tail vein of SD rats. Prothrombin time (PT) was measured by CoaguCheck XS (Roche). Warfarin and its metabolites was extracted by liquid-liquid distribution with ether and their concentration was measured by HPLC-MS/MS (Shimadzu). After two month of warfarin administration, 4 mg/kg of diphacinone disolved in corn oil was also administrated to EF bats.[Results and Discussion]After warfarin administration, prolonged PT was observed in both EF bats and SD rats, however, EF bats recovered to normal value earlier than SD rats. EF bats showed higher clearance of warfarin than SD rats and produced higher amounts of warfarin-metabolites than SD rats. These results suggested EF bats were less sensitive to warfarin than SD rats due to their high warfarin excretion ability. On the other hand, PT prolonged earlier in EF bats after diphacinone exposure, although plasma concentration of diphacinone was significantly lower in EF bats than in SD rats. These results indicated fruit bats showed different sensitivity to warfarin and diphacinone, although they have same mode of action.[Conclusion]This study revealed Egyptian fruit bats were relatively tolerant to warfarin but susceptible to diphacinone compared to rats. Further study with Bonin fruit bats is required for safety usage of ARs.

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Pharmacokinetic/pharmacodynamic analysis of first-generation anticoagulant rodenticide warfarin with Egyptian fruit bats (Rousettus aegyptiacus)

Shouta MM NAKAYAMA, Kosuke MANAGO, Kazuki TAKEDA, Ayuko MORITA, Yoshinori IKENAKA, Mayumi ISHIZUKA

SETAC North America 40th Annual Meeting

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Event date： 2019.11

Language：English Presentation type：Poster presentation

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抗凝血系殺鼠剤が小笠原諸島在来固有種に与える影響の評価

真砂皓大, 武田一貴, 中山翔太, 森田鮎子, 池中良徳, 石塚真由美

第25回日本環境毒性学会研究発表会

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Event date： 2019.9

Language：Japanese Presentation type：Oral presentation (general)

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In vivo analysis of rodenticides with Egyptian fruit bats

Kosuke MANAGO, Kazuki TAKEDA, Shouta MM NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Mayumi ISHIZUKA

The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)

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Event date： 2019.9

Language：English Presentation type：Poster presentation

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Investigation of mechanism of rodenticide resistance with closed colonies of rodenticide resistant rats from Tokyo

Kazuki Takeda, Yoshinori Ikenaka, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Tsutomu Tanikawa, Mayumi Ishizuka

the 15th International Congress of Toxicology

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Event date： 2019.7

Language：English Presentation type：Poster presentation

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環境汚染物質と淡水ガメ～環境中でのリスクアセスメント～

山村快哉, 武田一貴, 中山翔太, 川合佑典, 森田鮎子, 池中良徳, 小松謙之, 北山知代, 近藤理美, 石塚真由美

第６回淡水ガメ情報交換会

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Event date： 2019.2

Language：Japanese Presentation type：Oral presentation (general)

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DDTによる異物代謝酵素の遺伝子発現撹乱と健康影響評価

本平航大, 池中良徳, 池中良徳, BEYENE Yared, 中山翔太, 江口哲史, 武田一貴, WEPENER Victor, SMIT Nico, VAN VUREN Johan, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 2019

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Event date： 2019

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In Vivo Pharmacokinetic/pharmacodynamic analysis of first-generation anticoagulant rodenticide warfarin with Egyptian fruit bats (Rousettus aegyptiacus) for risk assessment of Bonin fruit bats (Pteropus pselaphon)

NAKAYAMA Shouta M.M., MANAGO Kosuke, TAKEDA Kazuki, MORITA Ayuko, IKENAKA Yoshinori, ISHIZUKA Mayumi

環境化学討論会要旨集(CD-ROM) 2019

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Event date： 2019

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東京都小笠原諸島における殺鼠剤散布のアオウミガメへのリスク評価

山村快哉, 武田一貴, 中山翔太, 川合佑典, 森田鮎子, 池中良徳, 小松謙之, 北山知代, 近藤理美, 石塚真由美

日本野生動物医学会大会・講演要旨集 2019

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Event date： 2019

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「基質結合部位からも活性残基からも離れたアミノ酸変異はどのように殺鼠剤抵抗性に寄与するのか?」ドッキングシミュレーション・分子動力学シミュレーションによるビタミンKエポキシド還元酵素の解析

武田一貴, 池中良徳, 池中良徳, FOURCHES Denis, 田中和之, 中山翔太, 谷川力, TRIKI Dhoha, LI Xinhao, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 2019

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Event date： 2019

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東京の殺鼠剤抵抗性ラットにおける殺鼠剤代謝能の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 石塚真由美

平成30年度内外環境応答・代謝酵素研究会

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Event date： 2018.11

Language：Japanese Presentation type：Oral presentation (general)

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アオウミガメ（Chelonia mydas）における第一世代抗血液凝固殺鼠剤ワルファリンの代謝能と感受性評価

山村快哉, 武田一貴, 中山翔太, 川合佑典, 池中良徳, 北山知代, 近藤理美, 石塚真由美

第29回日本ウミガメ会議与論島大会

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Event date： 2018.11

Language：Japanese Presentation type：Poster presentation

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Avian interspecific differences in rodenticide sensitivity: amino acid sequence and mRNA expression ratio of VKORC1 and VKORC1L1

Shouta M.M. NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Yusuke K. KAWAI, Kazuki TAKEDA, Hazuki MIZUKAWA, Chihiro ISHII, Keisuke SAITO, Yukiko WATANABE, Masaki ITO, Natsuo Ohsawa, Mayumi ISHIZUKA

SETAC North America 39th Annual Meeting

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Event date： 2018.11

Language：English Presentation type：Oral presentation (general)

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Avian interspecific differences in VKOR activity and inhibition: Amino acid sequence and mRNA expression ratio of VKORC1 and VKORC1L1

Shouta M.M. NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Yusuke K. KAWAI, Kazuki TAKEDA, Hazuki MIZUKAWA, Chihiro ISHII, Keisuke SAITO, Yukiko WATANABE, Masaki ITO, Natsuo Ohsawa, Mayumi ISHIZUKA

2018 International Meeting on 22nd MDO and 33rd JSSX

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Event date： 2018.10

Language：English Presentation type：Poster presentation

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Assessment of vitamin K epoxide reductase by in vitro kinetic analysis & in silico docking simulation for evaluation of rodenticide sensitivity

Kazuki TAKEDA, Yoshinori IKENAKA, Kazuyuki D. TANAKA, Shouta M.M. NAKAYAMA, Tsutomu TANIKAWA, Hazuki Mizukawa, Mayumi ISHIZUKA

The 6th Sapporo Summer Symposium for One Health (6th SaSSOH)

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Event date： 2018.9

Language：English Presentation type：Poster presentation

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Novel Mechanism of Rodenticide (warfarin) Resistance of Wild Rats in Tokyo ~ Enhanced Pentose Phosphate Pathway Causes Rapid Metabolism of Warfarin ~

Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

Society of Toxicology’s 57th Annual Meeting and ToxExpo

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Event date： 2018.3

Language：English Presentation type：Poster presentation

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HPLC-APCI-MSを用いたビタミンKの網羅的検出とin vitroビタミンKエポキシド還元酵素活性の評価

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 2018

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Event date： 2018

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殺鼠剤に弱い野生動物種を探る:標的分子ビタミンKエポキシド還元酵素(VKOR)の鳥類における特徴および種差

中山翔太, 森田鮎子, 池中良徳, 石井千尋, 水川葉月, 武田一貴, 川合佑典, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 石塚真由美

日本野生動物医学会大会・講演要旨集 2018

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Event date： 2018

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スーパーラットは何が「スーパー」?東京の殺鼠剤抵抗性ラットにおける抵抗性獲得機序の解析

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 2018

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Event date： 2018

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What Makes Super Rats "Super"? ~Investigation of Mechanisms of Rodenticide Resistance~

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D., NAKAYAMA Shouta M.M., TANIKAWA Tsutomu, MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 2018 The Japanese Society of Toxicology

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Event date： 2018

Language：Japanese

[in Japanese] 

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Novel investigation of mechanism of rodenticide resistance ~ enhanced pentose phosphate pathway causes rapid metabolism of warfarin ~

Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

The 5th Sapporo Summer Seminar for One Health (SaSSOH)

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Event date： 2017.9

Language：English Presentation type：Oral presentation (general)

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東京由来殺鼠剤抵抗性ラットを用いた新規抵抗性獲得機序の解明 ~ペントースリン酸経路の亢進による殺鼠剤代謝能の活性化~

武田一貴, 池中良徳, 田中和之, 谷川力, 中山翔太, 水川葉月, 石塚真由美

第160回日本獣医学会学術年会

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Event date： 2017.9

Presentation type：Oral presentation (general)

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Super rat (Rodenticide Resistant Rats) is super metabolizer of warfarin ~revealed by in situ, in vitro approach~

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D, NAKAYAMA Shouta M.M, TANIKAWA Tsutomu, MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 2017 The Japanese Society of Toxicology

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Event date： 2017

Language：Japanese

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In situ肝灌流試験によるワルファリン抵抗性クマネズミでのワルファリン代謝能の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 2017

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Event date： 2017

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東京由来殺鼠剤抵抗性ラットを用いた新規抵抗性獲得機序の解明:ペントースリン酸経路の亢進による殺鼠剤代謝能の活性化

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本獣医学会学術集会講演要旨集 2017

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Event date： 2017

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東京のワルファリン抵抗性ラットの抵抗性獲得機序の解明:ペントースリン酸経路の亢進を通したワルファリン代謝の活性化

武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本生化学会大会(Web) 2017

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Event date： 2017

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Investigation of Warfarin Metabolic Ability of Warfarin Resistant Roof Rats by in situ Liver Perfusion

Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

The 4th Sapporo Summer Seminar for One Health (SaSSOH)

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Event date： 2016.9

Language：English Presentation type：Poster presentation

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Pharmacokinetic analysis of warfarin resistant roof rats (Rattus rattus) in Tokyo Invited

Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka

8th International Toxicology Symposium in Africa

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Event date： 2016.8

Language：English Presentation type：Poster presentation

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In situ肝灌流モデルによる殺鼠剤抵抗性野生クマネズミにおけるワルファリン代謝能の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

日本分子生物学会年会プログラム・要旨集(Web) 2016

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Event date： 2016

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Investigation of pharmacokinetics / pharmacodynamics status in a closed colony of warfarin resistant rats (Rattus rattus) from Tokyo

TAKEDA Kazuki, IKENAKA Yoshinori, TANAKA Kazuyuki D., TANIKAWA Tsutomu, NAKAYAMA Shouta M.M., MIZUKAWA Hazuki, ISHIZUKA Mayumi

Annual Meeting of the Japanese Society of Toxicology 2016 The Japanese Society of Toxicology

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Event date： 2016

Language：Japanese

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野生齧歯類におけるワルファリン抵抗性獲得機序の解明

武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美

環境化学討論会要旨集(CD-ROM) 2015

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Event date： 2015

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Docking-based in silico analysis of ergot alkaloid–monoamine receptor interactions

Koshiro Tatsuta, Teppei Hayama, Ryo Kamata, Kazuki Takeda

8th Spring Meeting of the Japanese Society of Veterinary Pharmacology and Toxicology 2026.2

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Language：Japanese Presentation type：Oral presentation (general)

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Proteome-wide molecular docking to screen for novel vitamin K target candidates

Teppei Hayama, Ryo Kamata, Kazuki Takeda

8th Spring Meeting of the Japanese Society of Veterinary Pharmacology and Toxicology 2026.2

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Language：Kannada Presentation type：Oral presentation (general)

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AlphaFold-based modeling of estrogen receptor in agonist- and antagonist-bound states

Maki Kitaoka, Yuka Nakamura, Teppei Hayama, Ryo Kamata, Kazuki Takeda

8th Spring Meeting of the Japanese Society of Veterinary Pharmacology and Toxicology 2026.2

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Language：Japanese Presentation type：Oral presentation (general)

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Proposal and Accuracy Evaluation of a Proteome-Wide Chemical Target Identification Method Based on Molecular Docking: The Binding-Proteomics Approach

Kazuki Takeda

8th Workshop on Drug Toxicity Mechanisms organized by the Japanese Society of Toxicology (JSOT) 2025.12

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Integrative Analysis of VKORC1 Polymorphisms Targeted by Warfarin-Type Rodenticides: Molecular Dynamics Simulations and Machine Learning–Based Detection of Resistance Mutations

Moyu Miyamae, Ryo Kamata, Kazuki Takeda

The 168th Annual Meeting of The Society of Japanese Veterinary Medical Sciences 2025.9

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Binding-Proteomics: Development of a Comprehensive Chemical Target Prediction Method Using Proteome-Wide Molecular Docking Against AlphaFold-Predicted Protein Structures

Teppei Hayama, Rin Sugawara, Ryo Kamata, Masakazu Sekijima, Kazuki Takeda

168th Annual Meeting of the Japanese Society of Veterinary Science 2025.9

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Prediction of Ligand Binding Affinity to the Estrogen Receptor (ER) Using Protein Structure Prediction and Molecular Docking Simulations

Yuka Nakamura, Teppei Hayama, Rin Sugawara, Ryo Kamata, Kazuki Takeda

168th Annual Meeting of the Japanese Society of Veterinary Science 2025.9

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Presentation type：Oral presentation (general)

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Is Paper Recycling Truly Eco-Friendly? Evaluating the Toxic Effects of Recycled Paper Wastewater Using Zebrafish

Kazuki Takeda, Hina Saito, Masanori Terasaki, Akira Kubota, Akifumi Eguchi, Ryo Kamata

The 11th Zebrafish and Medaka Drug Discovery Conference 2025.11

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Alcohol and Organophosphate Metabolic Capacity in Elephants: A Study Based on Gene Expression, Enzymatic Dynamics, and Structural Analysis

Ayuko Morita, Kanami Watanabe, Poapolathep Amnar, Poapolathep Saranya, Saengtienchai Aksorn, Khidkhan Kraisiri, Sripiboon Supaphen, Srikulnath Kornsorn, Thitaram Chatchote, Somgid Chaleamcha, Angkawanish Taweepoke, Langkaphin Warangkhana, Mitsuki Kondo, Kazuki Takeda, Yoshinori Ikenaka, Shouta MM Nakayama, Mayumi Ishizuka

The 48th Annual Meeting of the Molecular Biology Society of Japan (MBSJ) 2025.12

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Exploring Antiviral Drug Interactions with Elephant Endotheliotropic Herpesvirus through Molecular Docking Simulation

Ayuko Morita, Kazuki Takeda, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka

21st International Elephant Conservation and Research Symposium 2025.12

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Reproductive Toxicity Assessment of Benzyl 2-Naphthyl Ether (BNE), a Contaminant Derived from Recycled Paper Mill Effluent, Using a Zebrafish Exposure Assay

Hina Saito, Kazuki Takeda, Masanori Terasaki, Akifumi Eguchi, Akira Kubota, Ryo Kamata

8th Workshop on Drug Toxicity Mechanisms organized by the Japanese Society of Toxicology (JSOT) 2025.12

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Evaluation of Testicular and Sperm Toxicity in Mice Following Mixed Exposure to Environmentally Relevant Concentrations of Perfluoroalkyl Substances (PFASs)

Aki Kanai, Wakana Yoshimura, Chihiro Kanno, Hiromi Kusaka, Makoto Sugiyama, Masashi Nagano, Ryo Kamata, Kazuki Takeda

8th Workshop on Drug Toxicity Mechanisms organized by the Japanese Society of Toxicology (JSOT) 2025.12

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Toxicological Evaluation of Benzyl 2-Naphthyl Ether (BNE), a Contaminant Derived from Recycled Paper Mill Effluent, Using a Zebrafish Exposure Assay

Hina Saito, Kazuki Takeda, Masanori Terasaki, Akifumi Eguchi, Akira Kubota, Ryo Kamata

The 168th Annual Meeting of The Society of Japanese Veterinary Medical Sciences 2025.9

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Analysis of Factors Affecting Stunning Success and the Relationship Between Cattle Behavioral Responses During Slaughter and Meat Quality Traits

Sawa Ogino, Kazuki Takeda, Hiroshi Miura, Yosuke Maeda, Fumiaki Takahashi

168th Annual Meeting of the Japanese Society of Veterinary Science 2025.9

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Structural Prediction and Identification of the Substrate Recognition Site of Vitamin K–Dependent Carboxylase (GGCX): An in silico Analysis Using AlphaFold

Konoha Inui, Teppei Hayama, Ryo Kamata, Kazuki Takeda

168th Annual Meeting of the Japanese Society of Veterinary Science 2025.9

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ESTIMATING SUSCEPTIBILITY OF RODENTICIDE BY MOLECULAR DYNAMICS & MACHINE LEARNING USING 3D STRUCTURES OF VITAMIN K EPOXIDE REDUCTASE Invited

Kazuki Takeda, Nobuaki Yasuo, Kanami Watanabe, Ryo Sato, Ryo Kamata, Masakazu Sekijima

The Pure and Applied Chemistry International Conference 2022 2022.6

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Drug Discovery Chemistry and Toxicology in Age of Post-AlphaFold2 Invited

Kazuki Takeda

The 25th Continuing Education Course of the Japanese Society of Toxicology 2024.7

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Pharmacokinetics/dynamics analysis of anticoagulant rodenticides with Egyptian fruit bats (Rousettus aegyptiacus)

Kazuki Takeda, Kosuke Manago, Shouta MM Nakayama, Ayuko Morita, Yoshinori Ikenaka, Mayumi Ishizuka

International Symposium on Chemical Hazard in Wildlife 2020.2

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Sensitivity of turtles to anticoagulant rodenticides: in vivo and in vitro sensitivity evaluation in green sea turtles (Chelonia mydas mydas) in the Ogasawara Islands

Shouta MM Nakayama, Yoshiya Yamamura, Kazuki Takeda, Yusuke Kawai, Ayuko Morita, Yoshinori Ikenaka, Noriyuki Komatsu, Chiho Kezuka, Mari Taniguchi, Chiyo Kitayama, Satomi Kondo, Mayumi Ishizuka

International Symposium on Chemical Hazard in Wildlife 2020.2

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Development of Lithocholic Acid Derivatives with High Vitamin D Activity and Their Application to Rodenticides

Hiroyuki Kagechika, Satoru Nagaoka, Kazuki Takeda, Aya Tanatani

The 46th Annual Meeting of the Bile Acid Research Society 2025.11

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Rodenticide Use in Japan – Super Rats in Tokyo and Susceptibility of Invasive Black Rats to Rodenticides on Chichijima, Ogasawara Islands Invited

Kazuki Takeda

The Mammal Society of Japan Annual Meeting 2025 2025.8

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Language：Japanese Presentation type：Symposium, workshop panel (public)

Workshop: Invasive Rodent Issues and Rodenticides on Islands

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Prediction of Anticoagulant Rodenticide Resistance through VKORC1 Variant Analysis Using Molecular Dynamics and Machine Learning

Moyu Miyamae, Ryo Kamata, Kazuki Takeda

2nd Meeting of Researchers of Kitasato Institute (DocTALK) 2025.8

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Proposal and accuracy assessment of comprehensive molecular docking-based target discovery using binding proteomics analysis, and its applicability to adductomics Invited

Kazuki Takeda

The 3rd Symposium of the Covalent Chemical Sciences Division, Japanese Society of Toxicology 2025.9

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Presentation type：Symposium, workshop panel (nominated)

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From Structure to Target: Exploring the AlphaFold-Predicted Proteome

Kazuki Takeda

3rd Annual General Meeting of the Association for Complex Life System Order 2025.9

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Development of an In Silico Target Protein Prediction Method via Comprehensive Molecular Docking to AlphaFold-Predicted Proteome Structures: A Case Study on Puberulic Acid

Teppei Hayama, Rin Sugawara, Ryo Kamata, Masakazu Sekijima, Kazuki Takeda

4th Joint Conference on Environmental Chemicals 2025.7

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Development of in silico target protein prediction pipeline by comprehensive molecular docking on the complete AlphaFold Proteome

Teppei Hayama, Rin Sugawara, Ryo Kamata, Masakazu Sekijima, Kazuki Takeda

2nd Meeting of Researchers of Kitasato Institute (DocTALK) 2025.8

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Testicular Toxicity Assessment of a Nine-Component PFAS Mixture at Human-Relevant Daily Exposure Levels in Mice

Wakana Yoshimura, Hiromi Kusaka, Chihiro Kanno, Makoto Sugiyama, Masashi Nagano, Ryo Kamata, Kazuki Takeda

38th Kitasato University Bioscience Forum 2025.8

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Molecular docking simulation of anti-herpesvirus drugs against elephant endotheliotropic herpesvirus (EEHV)

Ayuko Morita, Kazuki Takeda, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka

The 31st Annual Meeting of the Japanese Society of Wildlife Medicine 2025.9

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Binding Proteomics: A Comprehensive Target Discovery Approach via Molecular Docking across the AlphaFold Structural Proteome Invited

Kazuki Takeda

CBI Society Annual Meeting 2025 2025.10

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Summary of Career Path Survey at the 51st JSOT Annual Meeting Based on Machine Learning Analysis Invited

Kazuki Takeda

The 52nd Annual Meeting of Japanese Society of Toxicology 2025.7

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Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Toxicology × Emerging Technologies: Rethinking the Future of Toxicology – Introduction Invited

Kazuki Takeda

The 52nd Annual Meeting of Japanese Society of Toxicology 2025.7

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Language：Japanese Presentation type：Symposium, workshop panel (nominated)

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Accuracy Assessment of Binding Proteomics: A Comprehensive Molecular Docking-Based Target Identification Method

Rin Sugawara, Teppei Hayama, Ryo Kamata, Kazuki Takeda

The 52nd Annual Meeting of Japanese Society of Toxicology 2025.7

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Identification of Potential Toxicity Targets of Puberulic Acid via Comprehensive Molecular Docking “Binding Proteomics” Analysis on the Protein Structure Proteome

Kazuki Takeda, Teppei Hayama, RIンSugawara, Masakazu Sekijima, Ryo Kamata

The 52nd Annual Meeting of Japanese Society of Toxicology 2025.7

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Application of the Non-Secosteroidal Vitamin D Derivative Dcha-20 as a Rodenticide

Miyuna Yoshiwara, Chiharu Ogura, Misaki Fukamatsu, Satoru Nagaoka, Kana Okamura, Hiroyuki Kagechika, Ryo Kamata, Aya Tanatani, Kazuki Takeda

77 2025.6

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Development of Derivatives of Vitamin K3 with Enhanced 3CL Protease Inhibitory Activity

Miki Sasajima, Mika Okamoto, Ryohto Oharazawa, Mayu Hayamawa, Kazuki Takeda, Hirohisa Hirota, Masanori Baba, Yoshitomo Suhara

77th Annual Meeting of The Vitamin Society of Japan 2025.6

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Rodenticidal Effect and Mechanistic Elucidation of Dcha-20, a Lithocholic Acid Derivative with a Novel Mode of Action

Misaki Fukamatsu, Chiharu Ogura, Satoru Nagaoka, Kana Okamura, Miyuna Yoshiwara, Hiroyuki Kagechika, Ryo Kamata, Aya Tanatani, Kazuki Takeda

4th Joint Conference on Environmental Chemicals 2025.7

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Rodenticidal Effect and Toxicity Mechanism of Dcha-20, a Novel Vitamin D Receptor Agonist with a Lithocholic Acid Scaffold

Misaki Fukamatsu, Chiharu Ogura, Satoru Nagaoka, Kana Okamura, Miyuna Yoshiwara, Hiroyuki Kagechika, Ryo Kamata, Aya Tanatani, Kazuki Takeda

The 52nd Annual Meeting of Japanese Society of Toxicology 2025.7

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Development of an Odor Prediction Model Using Molecular Docking Simulations and Machine Learning

Koki Takaguchi, Keita Yamauchi, Akifumi Eguchi, Kazuki Takeda

4th Joint Conference on Environmental Chemicals 2025.7

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Evaluation of Testicular Toxicity in Mice Induced by Mixed Exposure to Nine Linear Perfluoroalkyl Compounds at Levels Equivalent to Daily Human Intake

Wakana Yoshimura, Hiromi Kusaka, Chihiro Kanno, Makoto Sugiyama, Masashi Nagano, Ryo Kamata, Kazuki Takeda

4th Joint Conference on Environmental Chemicals 2025.7

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AlphaFold2全プロテオーム予測構造に対する網羅的分子ドッキングによる「ドッキングプロテオミクス解析」の基礎的構築

菅原琳, 羽山哲平, 鎌田亮, 武田一貴

第47回日本分子生物学会年会 2024.11

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Unraveling Drug Sensitivity, Species Differences, and Individual Variability of Vitamin K Cycle-Related Proteins through Structural Science Invited

Kazuki Takeda

77th Annual Meeting of The Vitamin Society of Japan 2025.6

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Language：Japanese Presentation type：Oral presentation (invited, special)

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A Novel Species-Sensitive Animal Species Difference Assessment Method for Anti-Haemagglutinating Rodenticides by Machine Learning of Target Molecular Structure Modeling

Moyu Miyamae, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

7th Spring Meeting of the Japanese Society of Veterinary Pharmacology and Toxicology 2025.2

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Evaluation of species differences in susceptibility to anticoagulant rodenticides based on in silico protein structure analysis Invited

Moyu Miyamae, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

The Pure and Applied Chemistry International Conference (PACCON) 2025 2025.2

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Rodenticidal Activity of the Lithocholic Acid Derivative Dcha-20 Invited

Aya Tanatani, Kana Okamura, Chiharu Ogura, Kazuki Takeda, Satoru Nagaoka, Hiroyuki Kagechika

77th Annual Meeting of The Vitamin Society of Japan 2025.6

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Structural characterization of the human olfactory receptor superfamily

Keita Yamauchi, Koki Takaguchi, Teppei Hayama, Rin Sugawara, Ryo Kamata, Akifumi Eguchi, Kazuki Takeda

7th Spring Meeting of the Japanese Society of Veterinary Pharmacology and Toxicology 2025.3

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Trends in Vitamins and Rodenticides Based on Structural Biology Invited

Kazuki Takeda

2024.11

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Presentation type：Symposium, workshop panel (nominated)

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Pharmacokinetic/pharmacodynamic analysis of first-generation anticoagulant rodenticide warfarin with Egyptian fruit bats (Rousettus aegyptiacus) for risk assessment of Bonin fruit bats (Pteropus pselaphon)

Ayuko Morita, Kazuki Takeda, Kosuke Manago, Shouta M.M. Nakayama, Yusuke Kawai, Yoshinori Ikenaka, Mayumi Ishizuka

International CHEMICAL HAZARD Symposium 2019 2019.3

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Novel Mechanism of Rodenticide (warfarin) Resistance of Wild Rats in Tokyo ~ Enhanced Pentose Phosphate Pathway Causes Rapid Metabolism of Warfarin ~,

Kazuki TAKEDA, Yoshinori IKENAKA, Kazuyuki D. TANAKA, Shouta M.M. NAKAYAMA, Tsutomu TANIKAWA, Mayumi ISHIZUKA

2nd Chemical Hazard Symposium, 2018.12

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Comparison of two reducing agent dithiothreitol and tris(3-hydroxypropyl)phosphine for in vitro assay of vitamin K epoxide reductase (VKOR)

Kazuki Takeda, Ayuko Morita, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka

International CHEMICAL HAZARD Symposium 2019 2019.3

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体内動態および行動毒性試験から見た動物用医薬品フィプロニルの影響評価

鈴木智裕, 池中良徳, 武田一貴, 中山翔太, 水川葉月, 一瀬貴大, 佐々木東, 笹岡一慶, 滝口満喜, 石塚真由美

第162回日本獣医学会学術集会 2019.9

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効率的な毒性理解・回避低減のための複雑系/単純系in vitro評価モデルの選択 Invited

浜村えり, 白井勇司, 井口拓馬, 武田一貴, 藤本和則, 土屋由美

第51回日本毒性学会学術年会 2024.7

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Presentation type：Symposium, workshop panel (nominated)

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スーパーラットは高NADPH産生能を持つ～東京の殺鼠剤抵抗性ラットにおける抵抗性獲得機序の解明～

武田一貴, 池中良徳, 田中和之, 谷川力, 中山翔太, 水川葉月, 石塚真由美

第3回北大部局間横断シンポジウム 2018.1

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Language：Japanese Presentation type：Poster presentation

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Toxicity Assessment Using the Structural Proteome in the Post-AlphaFold2 Era Invited

Kazuki Takeda

Digital Toxicology Conference 2024.7

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Language：Japanese Presentation type：Oral presentation (invited, special)

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小笠原諸島における殺鼠剤散布が野生動物に及ぼす影響の解明

中山翔太, 川合佑典, 武田一貴, 池中良徳

小笠原諸島返還50周年記念みんなの近くの世界遺産＜返還50周年記念講演会＞ 2018.9

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Language：Japanese Presentation type：Poster presentation

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Search for Molecules Responsible for Differences in Drug Efficacy between cis-trans Isomers of Warfarin Rodenticides

Moyu Miyamae, Satoru Nagaoka, Sanae Yamamoto, Ryo Kamata, Kazuki Takeda

6th International Chemical Hazard Symposium 2024.3

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Awards

the 2024 Best Paper in the Veterinary Medicine

2025.4 Faculty of Veterinary Medicine, Kitasato University Assessment of the Aryl Hydrocarbon Receptor-Mediated Effects of Aromatic Sensitizers in Paper Recycling Effluent Employing Zebrafish Embryos and in Silico Docking.

Kazuki Takeda, Ryo Kamata

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Royal Society of Chemistry Award

2021.6 Japan Society for Environmental Chemistry Estimating susceptibility of rodenticide by molecular dynamics & machine learning using 3D structure of vitamin K epoxide reductase

Kazuki Takeda

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Award type：International academic award (Japan or overseas)

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Best Graduate Student Award, the 45th Annual Meeting of the Japanese Society of Toxicology

2018.7 The Japanese Society of Toxicology What Makes Super Rats "Super"? ～Exploring the Mechanism of Acquiring Resistance in Rodenticide-Resistant Black Rats Found in Tokyo

Kazuki Takeda

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SOT 57th Annual Meeting and ToxExpo, Comparative & Veterinary specialty section, Graduated Student Travel Award

2018.3 Society of Toxicology Novel Mechanism of Rodenticide (warfarin) Resistance of Wild Rats in Tokyo -Enhanced Pentose Phosphate Pathway Causes Rapid Metabolism of Warfarin -

Kazuki Takeda

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SOT 57th Annual Meeting and ToxExpo, Molecular and Systems Biology SS Graduated Student Honorable mention

2018.3 Society of Toxicology Novel Mechanism of Rodenticide (warfarin) Resistance of Wild Rats in Tokyo -Enhanced Pentose Phosphate Pathway Causes Rapid Metabolism of Warfarin –

Kazuki Takeda

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5th SaSSOH (Sapporo Summer Symposium for One Health) The best oral presentation award

2017.9 Graduate School of Veterinary Medicine, Hokkaido University Novel investigation of mechanism of rodenticide resistance ~ enhanced pentose phosphate pathway causes rapid metabolism of warfarin ~

Kazuki Takeda

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Best Presentation Award for Young Scientists at the 26th Annual Meeting of the Japan Society of Environmental Chemistry

2017.6 The Japan Society of Environmental Chemistry Investigation of hepatic warfarin metabolism activity in rodenticide-resistant black rats (Rattus rattus) in Tokyo by in situ liver perfusion.

Kazuki Takeda

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Best Presentation Award for Young Scientists, 43rd Annual Meeting of the Japanese Society of Toxicology

2016.7 The Japanese Society of Toxicology Pharmacokinetic Analysis of Anticoagulant Rodenticide Warfarin using Rodenticide Resistant Black Rats from Tokyo

Kazuki Takeda

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Best Student Presentation Award at the 24th Annual Meeting of the Japan Society of Environmental Chemistry

2015.6 The Japan Society of Environmental Chemistry Investigation of the Mechanism of Acquisition of Warfarin Resistance in Wild Rodents

Kazuki Takeda

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Research Projects

Cross-Class Elucidation of Resistance Mechanisms in the Era of Ineffective Rodenticides

Grant number：26K01903 2026.4 - 2030.3

Japan Society for the Promotion of Science (JSPS) Grant in Aid for Scientific Research (KAKENHI) Grant in Aid for Scientific Research (B)

Kazuki TAKEDA

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Authorship：Principal investigator

Grant amount：\18200000 （ Direct Cost: \14000000 、 Indirect Cost：\4200000 ）

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肝星細胞由来の接着関連分泌因子を介した肝炎病態制御機構の解明

Grant number：26K09839 2026.4 - 2029.3

日本学術振興会科学研究費助成事業基盤研究(C)

湯浅秀人, 松原勤, 宇留島隼人, 武田一貴

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Authorship：Coinvestigator(s)

Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

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Study on toxicity assessment based on the muscle-contractile effects of ergot alkaloids

2025.12 - 2027.12

Ministry of Agriculture, Forestry and Fisheries (MAFF) FY2025 Regulatory Science Research Promotion Commissioned Project (Short-term Issue-Solving Type)

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Authorship：Coinvestigator(s)

https://www.maff.go.jp/j/syouan/seisaku/regulatory_science/rsr7-1.html#result

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Structural Science-Based Definition of Chemical Susceptibility in Wildlife

Grant number：JPMJBY24E6 2025.9 - 2030.8

Japan Science and Technology Agency (JST) BOOST: Broadening Opportunities for Outstanding Young Researchers in Strategic Areas – Next-Generation AI Human Resource Development Program

Kazuki Takeda

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Authorship：Principal investigator

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Development of eradication methods for invasive rodents in the Ogasawara Islands

Grant number：JPMEERF20254001 2025.4 - 2028.3

Environmental Restoration and Conservation Agency (ERCA) Environment Research and Technology Development Fund Research on Environmental Problems

Masashi Katayama, Shouta Nakayama, Takuma Hashimoto, Yusuke Kawai, Kazuki Takeda, Kaoruko Mizukawa, Akifumi Eguchi, Takahiro Morozawa, Akemi Ori, Ryuichi Minato, Shuki Kikuchi

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Authorship：Coinvestigator(s)

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Establishment of a Novel Approach for Identifying Toxicological Target Proteins Using a Generative “Drawing AI”

2025.4 - 2026.3

Kitasato Institute Grant-in-Aid for Academic Research, Kitasato University, FY2025 (38th Term)

Kazuki Takeda

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Authorship：Principal investigator

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新規ビタミンＤ誘導体Ｄｃｈａ－２０の殺鼠剤開発

Grant number：JPMJTR244A 2024.12 - 2029.3

国立研究開発法人科学技術振興機構（JST） A-STEP（本格フェーズ）

棚谷綾, 武田一貴, 影近弘之, 長岡慧

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Development and clinical application of a non-invasive drug sensitivity assessment method for ex situ conservation of threatened avian species

Grant number：23K18531 2023.6 - 2026.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

Akira Kubota, Shin-ichi Ikushiro, Rumi Tanoue, Kazuki Takeda, Yusuke K Kawai

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Authorship：Coinvestigator(s)

Grant amount：\6240000 （ Direct Cost: \4800000 、 Indirect Cost：\1440000 ）

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3D画像解析を活用した肉用牛におけると畜時の適切な姿勢および放銃位置の探索

2023.4 - 2026.3

公益財団法人伊藤記念財団研究助成

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Authorship：Coinvestigator(s)

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Establishment of a Novel Method for Predicting Off-Target Toxicity by Comprehensive Binding Simulation

Grant number：23K14091 2023.4 - 2025.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) Grant-in-Aid for Early-Career Scientists

Kazuki Takeda

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Authorship：Principal investigator

Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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Analysis of human and animal toxicological effects and mechanisms of environmental pollution in Africa

Grant number：22KK0163 2022.10 - 2026.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)) Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

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Grant amount：\20020000 （ Direct Cost: \15400000 、 Indirect Cost：\4620000 ）

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Development of a novel evaluation method using cultured cells to assess the effects of environmental pollutants on wildlife.

Grant number：JPMEERF20214R02 2021.7 - 2024.3

Environmental Restoration and Conservation Agency, ERCA Environment Research and Technology Development Fund

Masafumi Katayama, Shouta MM Nakayama, Kazuki Takeda

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Adaptive evolution/change in mammals to exogenous chemicals (xenobiotics)

Grant number：21H04919 2021.4 - 2026.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A) Grant-in-Aid for Scientific Research (A)

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Authorship：Coinvestigator(s)

Grant amount：\41600000 （ Direct Cost: \32000000 、 Indirect Cost：\9600000 ）

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Establishment of an evaluation method of a chemical-susceptibility for wildlife using counter-QSAR and machine learning

Grant number：21K14988 2021.4 - 2023.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists Grant-in-Aid for Early-Career Scientists

Kazuki TAKEDA

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Authorship：Principal investigator

Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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Establishment of an evaluation method of a chemical-susceptibility for wildlife using counter-QSAR and machine learning

Grant number：20J01553 2020.4 - 2023.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows Grant-in-Aid for JSPS Fellows

Kazuki TAKEDA

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\11700000 （ Direct Cost: \9000000 、 Indirect Cost：\2700000 ）

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Whole investigation of mechanism of rodenticide resistance of wild rodents and application to novel rodenticide

Grant number：17J05808 2017.4 - 2020.3

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows Grant-in-Aid for JSPS Fellows

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\3100000 （ Direct Cost: \3100000 ）

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Other

[Mentored Student Award] 38th Kitasato University Bioscience Forum, Young Scientist Award

2025.8

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Prediction of Anticoagulant Rodenticide Resistance through VKORC1 Variant Analysis Using Molecular Dynamics and Machine Learning, Moyu Miyamae

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[Mentored Student Award] 38th Kitasato University Bioscience Forum, Young Scientist Award

2025.8

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Testicular Toxicity Assessment of a Nine-Component PFAS Mixture at Human-Relevant Daily Exposure Levels in Mice, Wakana Yoshimura

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[Mentored Student Award] Young Investigator Award, Japanese Society of Environmental Toxicology: 4th Joint Conference on Environmental Chemicals

2025.7

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Evaluation of Testicular Toxicity in Mice Induced by Mixed Exposure to Nine Linear Perfluoroalkyl Compounds at Levels Equivalent to Daily Human Intake, Wakana Yoshimura

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[Mentored Student Award] Wellignton Laboratories Award: 4th Joint Conference on Environmental Chemicals

2025.7

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Rodenticidal Effect and Mechanistic Elucidation of Dcha-20, a Lithocholic Acid Derivative with a Novel Mode of Action, Misaki Fukamatsu

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[Mentored Student Award] American Chemical Society Award: 4th Joint Conference on Environmental Chemicals

2025.7

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Development of an In Silico Target Protein Prediction Method via Comprehensive Molecular Docking to AlphaFold-Predicted Proteome Structures: A Case Study on Puberulic Acid, Teppei Hayama

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[Mentored Student Award] Student Poster Presentation Award, 52nd Annual Meeting of the Japanese Society of Toxicology

2025.7

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Mechanistic Analysis of Dcha-20, a Novel Rodenticide with a Lithocholic Acid Scaffold, Misaki Fukamatsu

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[Mentored Student Award] 7th Pharmaceutical Toxicity Mechanisms Conference Excellent Presentation Award

2025.1

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"Development of an In Silico Evaluation Method for Chemical Binding Proteins Through Comprehensive Molecular Docking Simulation"
Student: Rin Sugawara
https://www.senkyo.co.jp/iyakudokuken/7th_shizuoka/contents/award.html

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[Mentored Student Award] 167th Annual Meeting of the Japanese Society of Veterinary Science, Pharmacology and Toxicology Section Excellent Presentation Award

2024.9

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"Evaluation of Testicular Toxicity Effects Due to Mixed Exposure of Nine Types of Perfluoroalkyl Substances (PFASs) at Everyday Intake Levels"
Student: Wakana Yoshimura
https://www.jsvetsci.jp/society/prize05_167.php#:~:text=%E5%90%89%E6%9D%91%20%E5%92%8C%E5%8A%A0%E8%8F%9C,%E5%BD%B1%E9%9F%BF%E3%81%AE%E8%A9%95%E4%BE%A1

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[Mentored Student Award] 167th Annual Meeting of the Japanese Society of Veterinary Science, Wildlife Science Section Excellent Presentation Award

2024.9

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"Prediction of the Pharmacokinetics of Anticoagulant Rodenticides in Wildlife Using Partial Differential Equation Modeling"
Student: Misaki Fukamatsu
https://www.jsvetsci.jp/society/prize05_167.php#:~:text=%E6%B7%B1%E6%9D%BE%20%E7%BE%8E%E5%92%B2,%E5%8B%95%E6%85%8B%E3%81%AE%E4%BA%88%E6%B8%AC

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[Mentored Student Award] 3rd Joint Conference on Environmental Chemicals, SETAC Award

2024.7

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"Pharmacokinetic and Pharmacodynamic (PK/PD) Analysis for Susceptibility Assessment of the Invasive Rat Population in Ogasawara Father Island to the Rodenticide Diphacinone"
Student: Kotaro Tanaka
https://j-ec.smartcore.jp/uploads/ckfinder/files/forum/jec_young_award.pdf

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[Mentored Student Award] 3rd Annual Meeting of the Joint Conference on Environmental Chemicals, Wellington Laboratories Award

2024.7

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"Evaluation of Species-Specific Susceptibility to Warfarin-like Rodenticides Based on In Silico Protein Structure Analysis"
Student: Moyu Miyamae
https://j-ec.smartcore.jp/uploads/ckfinder/files/forum/jec_young_award.pdf

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[Mentored Student Award] 51st Annual Meeting of the Japanese Society of Toxicology, Student Poster Presentation Award

2024.7

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"Evaluation of Aromatic Hydrocarbon Receptor (AhR) Activation by Sensitizer-Related Substances in Recycled Paper Mill Effluent"
Jiayan SUN
https://www.jsot2024.jp/contents/program.html

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[Mentored Student Award] 165th Annual Meeting of the Japanese Society of Veterinary Science, Wildlife Science Section Excellent Presentation Award

2022.9

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Sensitivity assessment of diphacinone by pharmacokinetic analysis in invasive black rats in the Bonin (Ogasawara) Archipelago, Japan
Student: Keita Shimizu
https://www.jsvetsci.jp/society/prize05_165.php#:~:text=%E6%B8%85%E6%B0%B4%20%E6%95%AC%E5%A4%AA,%E5%A4%A7%E5%AD%A6%20%E7%8D%A3%E5%8C%BB%E5%AD%A6%E9%83%A8

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[Mentored Student Award] 28th Annual Meeting of the Japanese Society of Wildlife Medicine, Best Oral Presentation Award

2022.9

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"Pharmacokinetic and Pharmacodynamic (PK/PD) Analysis for Susceptibility Assessment of the Invasive Rat Population on Ogasawara Father Island to the Rodenticide Diphacinone, Captured in 2022"
Student: Keita Shimizu
https://buneido-shuppan.com/jvmnews/article/jvm20220928-001#:~:text=%E6%9C%80%E5%84%AA%E7%A7%80%E8%B3%9E%EF%BC%9A%E6%B8%85%E6%B0%B4%E6%95%AC%E5%A4%AA(%E5%8C%97%E9%87%8C%E5%A4%A7%E5%AD%A6%E7%8D%A3%E5%8C%BB%E5%AD%A6%E9%83%A8%E6%AF%92%E6%80%A7%E5%AD%A6%E7%A0%94%E7%A9%B6%E5%AE%A4)

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[Mentored Student Award] Joint Conference of Three Environmental Chemical Societies, SETAC Award

2022.6

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"Evaluation of Aromatic Hydrocarbon Receptor (AhR) Activation by Sensitizer-Related Substances Using Zebrafish"
Student: Aoi Sarata
https://j-ec.smartcore.jp/uploads/ckfinder/files/30th_JEC_award.pdf

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Teaching Experience

Veterinary Medicine Fundamentals A/B

2026.6 Institution：Institute of Agriculture, Tokyo University of Agriculture and Technology

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Advanced Topics in Pharmaceutical Life Sciences

2025.11 Institution：Graduate School of Pharmaceutical Sciences, Kitasato University

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Biological Statistics

2025.9 Institution：School of Veterinary Medicine, Kitasato University

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Level：Undergraduate (specialized) Country：Japan

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Animal Environmental Health

2024.9 Institution：Department of Environmental Bioscience, School of Veterinary Medicine, Kitasato University

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Level：Undergraduate (specialized)

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Graduate School Special Lecture

2023.7 Institution：University of Shizuoka

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Level：Postgraduate

Application of AlphaFold2 for ultra-precise protein conformational prediction for toxicity evaluation -Drugs, environmental pollutants, and animal species differences

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Introduction to Veterinary Medicine Laboratory

2021.4 Institution：School of Veterinary Medicine, Kitasato University

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Veterinary Medical English

2020.9 Institution：School of Veterinary Medicine, Kitasato University

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Practical Class of Toxicology

2020.9 Institution：School of Veterinary Medicine, Kitasato University

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Graduation Thesis

2020.9 Institution：School of Veterinary Medicine, Kitasato University

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Chemical Hazard Control Expert Class

2020.7 Institution：Graduate School of Veterinary Medicine, Hokkaido University

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Level：Postgraduate Country：Japan

Practical Class of Computational Toxicology

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Social Activities

Is water a poison? Is avocado a poison? Everything is poison — by Paracelsus (Introduction to Toxicology)

Role(s)： Lecturer

Hachinohe Kita High School 2025.10

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Type：Visiting lecture

File： 2025模擬講義抜粋版.pdf

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Poisons ≡ drugs ∀chemicals; species differences in chemical sensitivity

Role(s)： Lecturer

2025.6

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Audience： High school students

Type：Visiting lecture

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Poisons ≡ drugs ∀chemicals; species differences in chemical sensitivity

Role(s)： Lecturer

Licenseacademy.Co.,Ltd Seibu Gakuen Bunri Senior High School 2024.7

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Audience： High school students

Type：Visiting lecture

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「こちら、ウミガメ研究チーム〜細胞×毒性×AI ラボから救え！小笠原の希少種」

Role(s)： Appearance,　Panelist,　Lecturer,　Demonstrator

北海道大学 CoSTEP 国立環境研究所生物多様性領域北海道大学大学院獣医学研究院毒性学教室北里大学獣医学部毒性学研究室認定NPO法人エバーラスティング・ネイチャー（ELNA）第124回サイエンス・カフェ札幌 × 環境研究総合推進費公開シンポジウム 2022.8

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Type：Science cafe

File： SC124_a5_omote_final_RGB-960x1362.jpg

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Media Coverage

[Cutting-edge Laboratory Challenge] Kitasato University School of Veterinary Medicine, Laboratory of Toxicology — Utilizing Artificial Intelligence to Predict the Effects of Chemical Substances. Newspaper, magazine

Daily Tohoku Shimbun,Inc. Daily Tohoku Cutting-edge Laboratory Challenge 2025.12

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Author：Other

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A New Technology to Evaluate and Predict without Killing WIldlife Promotional material

Leave a Nest Co., Ltd. someone vol.65 (winter 2023) Paracelsus's Future Travelogue pp8-9 2023.12

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https://lne.st/business/publishing/someone/

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SUPER RATS Internet

VICE JAPAN PEST KINGDOM https://www.vice.com/ja/article/j5zmnk/super-rats 2016.11

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Author：Other

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