Updated on 2026/08/14

写真a

 
TAJIMA KENYA
 
Organization
Institute of Future Science Earth-Life Science Institute Researcher
Title
Researcher
External link

Degree

  • 博士(理学)

Research Interests

  • Ribosome

  • Protein

  • RNA

  • Protein capsule

Research Areas

  • Life Science / Molecular biology

  • Life Science / Evolutionary biology

Education

  • The University of Tokyo   Graduate School of Science Doctoral Program   Chemistry course

    2017.4 - 2020.3

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    Country: Japan

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Research History

  • Institute of Science Tokyo   Earth-Life Science Institute, Institute of Future Science

    2024.10

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  • 日本学術振興会 特別研究員-PD

    2024.4

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  • Tokyo Institute of Technology   Earth-Life Science Institute   Researcher

    2022.9

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    Country:Japan

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  • 日本学術振興会 特別研究員-DC2

    2018.4 - 2020.3

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Papers

  • Efficient cell-free evolution of RNA polymerases by droplet microfluidics

    Naohiro Terasaka, Taro Furubayashi, Kenya Tajima, Hiroyuki Noji

    bioRxiv   2026.2

  • Cargo-Directed Assembly of Nonviral Nucleocapsid with Controlled Size

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    ACS Synthetic Biology   15 ( 3 )   1178 - 1186   2026.2

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    Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    DOI: 10.1021/acssynbio.5c00881

    PubMed

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  • Boosted cell-free gene expression for robust signal readout from a single-copy DNA template in microdroplets

    Taro Furubayashi, Naohiro Terasaka, Kenya Tajima, Hiroyuki Noji

    bioRxiv   2026.2

  • Cargo-directed assembly of nonviral nucleocapsid with controlled size

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    bioRxiv   2025.11

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    Publisher:openRxiv  

    Abstract

    Precise packaging of diverse cargo within self-assembling protein cages of defined size and shape is essential for many biotechnological applications, yet cellular expression offers limited control over loading. Here, we developed a system for in vitro cargo-directed reconstitution of a split, laboratory evolved nonviral nucleocapsid (spNC-4). Independently expressed and purified spNC- 4 capsid protein subunits were mixed and assembled with cargo molecules in a cooperative manner. As an authentic cargo, mRNA is packaged into a 30 nm-spheric nucleocapsid in vitro , closely matching to spNC-4 expressed in cells. In this system, a diverse range of cargo molecules, including cognate nucleocapsid mRNA, noncognate RNA, RNA complexed with positively supercharged fluorescent protein, and linear double-stranded DNA are encapsulated within the 30 nm-spheric nucleocapsids in vitro . Moreover, the packaging of 30 nm-spherical or rod-shaped DNA origamis as templates induce morphological alterations of the nucleocapsids, resulting in the formation of enlarged 60 nm-spherical structure or rod-shaped structure, respectively. This split-protein, cargo-dependent system provides versatile and programmable control over both composition and architecture of nonviral protein cages, creating a general platform for enzyme nanoreactors, targeted delivery, and vaccine development.

    DOI: 10.1101/2025.11.06.684264

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  • Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    Nucleic Acids Research   50 ( 5 )   2736 - 2753   2022.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gkac068

    Web of Science

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  • Comprehensive elucidation of effect of nascent peptide sequences and EF-G concentration on incorporation of prolines in translation Reviewed

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    Peptide Science   2018   103 - 103   2019

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    Authorship:Lead author   Language:English   Publishing type:Research paper (international conference proceedings)  

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  • Genetic code expansion via integration of redundant amino acid assignment by finely tuning tRNA pools

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    Current Opinion in Chemical Biology   46   212 - 218   2018.10

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cbpa.2018.07.010

    Web of Science

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  • Consecutive Elongation of D-Amino Acids in Translation

    Takayuki Katoh, Kenya Tajima, Hiroaki Suga

    Cell Chemical Biology   24 ( 1 )   46 - 54   2017.1

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.chembiol.2016.11.012

    Web of Science

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Books

  • ベンゾフラノインドリン真菌ペプチドの構造改変による抗腫瘍活性分子の同定

    田島研也( Role: Sole author)

    ファルマシア  2026.4 

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  • がん細胞早期発見を目指した大環状ペプチド蛍光プローブの開発

    田島研也, 菅裕明( Role: Joint author)

    医歯薬出版株式会社  2017 

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Presentations

  • In vitro assembly of a nonviral protein capsid and its morphological control mediated by versatile cargos

    Kenya TAJIMA, Yusuke SAKAI, Naohiro TERASAKA

    2025.9 

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    Event date: 2025.9

    Language:English   Presentation type:Poster presentation  

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  • In vitro nonviral protein capsid assembly with versatile cargos and controlled morphology Invited

    Kenya TAJIMA

    EMBO Fellows' Meeting 2025  2025.6 

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    Event date: 2025.6 - 2025.7

    Language:English   Presentation type:Oral presentation (invited, special)  

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  • In vitro nonviral protein capsid assembly with versatile cargos and controlled morphology Invited

    Kenya TAJIMA, Yusuke SAKAI, Naohiro Terasaka

    EMBO Fellows' Meeting 2025  2025.7 

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    Event date: 2025.6 - 2025.7

    Language:English   Presentation type:Poster presentation  

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  • In vitro nonviral protein capsid assembly with versatile cargos and controlled morphology Invited

    Kenya TAJIMA, Yusuke SAKAI, Naohiro TERASAKA

    Global insights in life science: EMBO meets WPI  2025.6 

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    Event date: 2025.6

    Language:English   Presentation type:Poster presentation  

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  • In vitro assembly of a protein capsule and cargo molecules into virus-like particles.

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    21st IUPAB & 62nd BSI joint congress 2024  2024.6 

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    Event date: 2024.6

    Language:English   Presentation type:Poster presentation  

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  • In vitro packaging of various cargo molecules into a non-viral protein cage.

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    The 24th Annual Meeting of the Protein Science Society of Japan  2024.6 

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    Event date: 2024.6

    Language:English   Presentation type:Poster presentation  

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  • In vitro assembly of protein capsule and cargo nucleic acid molecules into a virus-like particle.

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    Joint CO world & 12th ELSI Symposium  2024.1 

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    Event date: 2024.1

    Language:English   Presentation type:Poster presentation  

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  • Nucleic acids templated assembly of artificial virus-like particles

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    2023.9 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

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  • In vitro assembly of protein capsule and cargo molecules into a virus-like particle.

    Kenya Tajima, Naohiro Terasaka

    11th ELSI symposium  2023.3 

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    Event date: 2023.3

    Language:English   Presentation type:Poster presentation  

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  • Comprehensive elucidation of effect of nascent peptide sequences and EF-G concentration on incorporation of prolines in translation

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    10th International Peptide Symposium  2018.12 

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    Event date: 2018.12

    Language:English   Presentation type:Poster presentation  

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  • Comprehensive elucidation of effect of nascent peptide sequences and EF-G concentration on incorporation of prolines in translation

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    4th ICCPP Meeting  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Poster presentation  

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  • Comprehensive elucidation of effect of nascent chain sequences and EF-G concentration on incorporation of prolines in a translation system

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    The 13th Annual Meeting of Japanese Society for Chemical Biology  2018.6 

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    Event date: 2018.6

    Language:English   Presentation type:Poster presentation  

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  • EF-Gとペプチド新生鎖がペプチジル-tRNA脱落に及ぼす影響の解明

    田島 研也, 加藤 敬行, 菅 裕明

    第 5 回 バイオ関連化学シンポジウム若手フォーラム  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Poster presentation  

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  • Improvement of D-amino acid incorporation efficiency by suppressing peptidyl-tRNA drop-off and ribosome stalling

    Kenya Tajima, Takayuki Katoh, Hiroaki Suga

    2016.11 

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    Event date: 2016.11

    Language:English   Presentation type:Poster presentation  

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  • EF-Gに依存したペプチジル-tRNA脱落経路の解明

    田島研也, 加藤敬行, 菅裕明

    「細胞を創る」研究会 8.0  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Poster presentation  

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Research Projects

  • 酵素発現量を自律的に最適化する代謝経路の構築

    Grant number:26K17715  2026.4 - 2028.3

    日本学術振興会  科学研究費助成事業  若手研究

    田島 研也

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    Grant amount:\3770000 ( Direct Cost: \2900000 、 Indirect Cost:\870000 )

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  • 標的細胞選択的に細胞質へmRNAを送達するタンパク質カプセルの開発

    Grant number:24KJ1042  2024.4 - 2027.3

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    田島 研也

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    Authorship:Principal investigator 

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

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  • Development of the translation machinery to incorporate multiple D-amino acids into a peptide via alteration of the ribosome tunnel

    Grant number:18J12342  2018.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship:Principal investigator 

    Grant amount:\1900000 ( Direct Cost: \1900000 )

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