Organization
Institute of Integrated Research Biomedical AI Research Unit Specially Appointed Professor
Title
Specially Appointed Professor
External link
SUGIO SHIGETOSHI
Degree
-
Ph.D. ( 1985.3 Osaka University )
Research Areas
-
Life Science / Structural biochemistry / Protein Crystallography, Structural Biology, Protein Science, Drug Discovery
Education
-
Osaka University Graduate School of Pharmaceutical Sciences
1980.4 - 1985.3
Country： Japan
-
Tokyo University of Science Faculty of Pharmaceutical Sciences Department of Pharmaceutical Technochemistry
1976.4 - 1980.3
Country： Japan
Research History
-
Institute of Science Tokyo Biomedical AI Research Unit, Institute of Integrated Research Specially Appointed Professor
2024.10
-
Tokyo Institute of Technology BioMedical Artifical Intelligence Research Unit, Institute of Innovative Research Specially Appointed Professor
2022.4 - 2024.9
Country：Japan
-
Tokyo Institute of Technology Research Management Office, Institute of Innovative Research Specially Appointed Professor
2016.11 - 2022.3
-
Mitsubishi Chemical Corporation Department of Ion Exchange Resin Deputy General Manager
2016.4 - 2016.10
Country：Japan
-
Mitsubishi Chemical Corporation Laboratory for Ion Exchange Resin, Kurosaki Development Laboratories General Manager
2012.4 - 2016.3
Country：Japan
-
Mitsubishi Chemical Group Science and Technology Research Center, Inc. Biotechnology Laboratory Chief Scientist Group Leader
2007.4 - 2012.3
Country：Japan
-
Yokohama City University International Graduate School of Arts and Sciences Visiting Professor
2006.4 - 2016.3
Country：Japan
-
Zoegene Corporation Department of Structural Biology General Manager
2006.1 - 2007.3
Country：Japan
-
Kyoto University Graduate School of Agriculture Visiting Recturer
2005.10 - 2006.3
Country：Japan
-
Zoegene Corporation Structural Biology Business Unit Head
2005.5 - 2005.12
Country：Japan
-
Kyoto University Institute for Chemical Research Visiting Associate Professor
2003.4 - 2004.3
Country：Japan
-
Zoegene Corporation Structural Biology Group Group Manager
2002.1 - 2005.4
Country：Japan
-
Mitsubishi Chemical Corporation Science and Technology Research Center Senior Research Scientist
2001.4 - 2001.12
Country：Japan
-
Mitsubishi Chemical Corporation Yokohama Research Laboratories Senior Research Scientist
1997.4 - 2001.3
Country：Japan
-
The Green Cross Corporation The Central Research Laboratories Senior Research Scientist
1991.4 - 1997.3
Country：Japan
-
Brandeis University Rosenstiel Basic Medical Sciences Research Center Visiting Scientist
1990.4 - 1993.4
Country：United States
-
The Green Cross Corporation The Central Research Laboratory Research Scientist
1985.4 - 1991.3
Country：Japan
Professional Memberships
-
The Society of Chemical Engineers, Japan
2015.4 - 2016.3
-
The Protein Sciety
2003 - 2009
-
Protein Science Society of Japan
2000 - 2007
-
American Crystallographic Association
1992 - 2004
-
The Crystallographic Society of Japan
1980.7
-
The Biophisical Society of Japan
1980.7 - 2020.3
Committee Memberships
-
宇宙航空研究開発機構 有人宇宙ミッション本部 宇宙環境利用センター 高品質タンパク質結晶生成WG委員
2013 - 2016
Committee type：Government
-
日本学術振興会 産学協力研究委員会 量子ビーム融合化利用研究に関する先導的研究開発委員会幹事
2010 - 2011
Committee type：Government
-
宇宙航空研究開発機構 宇宙環境利用センター JEM応用利用推進委員会 タンパク質結晶精製実験選定評価WG委員
2009 - 2013
Committee type：Government
-
つくば構造生物産業利用推進共同体 企業委員
2006 - 2011
Committee type：Other
-
日本学術振興会 産学協力研究委員会 回折構造生物第１６９委員会 運営委員
2005 - 2011
Committee type：Academic society
-
蛋白質構造解析コンソーシアム 技術ＷＧ委員
2002 - 2007
Committee type：Other
-
SPring-8構造生物産業応用研究会 代表幹事
2000 - 2002
Committee type：Other
-
兵庫県微小結晶構造解析技術研究会 幹事
1997 - 2000
Committee type：Municipal
-
日本結晶学会 編集委員
1996 - 2000
Committee type：Academic society
-
科学技術振興機構 ライフサイエンスの革新を目指した構造生命科学と先端的基盤技術領域 さきがけ領域アドバイザー
Committee type：Government
Papers
-
Bioinformatic identification of cytochrome b5 homologues from the parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans highlights the crucial role of A. suum adult-specific secretory cytochrome b₅ in parasitic adaptation. International journal
Shinzaburo Takamiya, Muneaki Hashimoto, Toshihiro Mita, Takehiro Yokota, Yoshitaka Nakajima, Fumiyuki Yamakura, Shigetoshi Sugio, Tsutomu Fujimura, Takashi Ueno, Hiroshi Yamasaki
Parasitology international 65 ( 2 ) 113 - 20 2016.4
-
Cloning and structure-function analyses of quinolone- and acridone-producing novel type III polyketide synthases from Citrus microcarpa. International journal
Takahiro Mori, Yoshihiko Shimokawa, Takashi Matsui, Keishi Kinjo, Ryohei Kato, Hiroshi Noguchi, Shigetoshi Sugio, Hiroyuki Morita, Ikuro Abe
The Journal of biological chemistry 288 ( 40 ) 28845 - 58 2013.10
-
Crystal structure of GTPase domain fused with minimal stalks from human dynamin-1-like protein (Dlpl) in complex with several nucleotide analogues
Hiroyuki Kishida, Shigetoshi Sugio
Current Topics in Peptide and Protein Research 14 67 - 77 2013
-
Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus. International journal
Jing Chen, Hiroyuki Morita, Ryohei Kato, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe
Acta crystallographica. Section F, Structural biology and crystallization communications 68 ( Pt 3 ) 355 - 8 2012.3
-
Arg305 of Streptomyces L-glutamate oxidase plays a crucial role for substrate recognition. International journal
Tomohiro Utsumi, Jiro Arima, Chika Sakaguchi, Takashi Tamura, Chiduko Sasaki, Hitoshi Kusakabe, Shigetoshi Sugio, Kenji Inagaki
Biochemical and biophysical research communications 417 ( 3 ) 951 - 5 2012.1
-
Synthesis of unnatural alkaloid scaffolds by exploiting plant polyketide synthase. International journal
Hiroyuki Morita, Makoto Yamashita, She-Po Shi, Toshiyuki Wakimoto, Shin Kondo, Ryohei Kato, Shigetoshi Sugio, Toshiyuki Kohno, Ikuro Abe
Proceedings of the National Academy of Sciences of the United States of America 108 ( 33 ) 13504 - 9 2011.8
-
Functional expression of milligram quantities of the synthetic human serotonin transporter gene in a tetracycline-inducible HEK293 cell line. International journal
Hidehito Takayama, Shigetoshi Sugio
Protein expression and purification 76 ( 2 ) 211 - 20 2011.4
-
Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana. International journal
Hiroyuki Morita, Takahiro Mori, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio, Ikuro Abe
Acta crystallographica. Section F, Structural biology and crystallization communications 67 ( Pt 3 ) 409 - 11 2011.3
-
Structural basis for the one-pot formation of the diarylheptanoid scaffold by curcuminoid synthase from Oryza sativa. International journal
Hiroyuki Morita, Kiyofumi Wanibuchi, Hirohiko Nii, Ryohei Kato, Shigetoshi Sugio, Ikuro Abe
Proceedings of the National Academy of Sciences of the United States of America 107 ( 46 ) 19778 - 83 2010.11
-
Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids. International journal
Hiroyuki Morita, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio, Ikuro Abe
Acta crystallographica. Section F, Structural biology and crystallization communications 66 ( Pt 8 ) 948 - 50 2010.8
-
A structure-based mechanism for benzalacetone synthase from Rheum palmatum. International journal
Hiroyuki Morita, Yoshihiko Shimokawa, Michikazu Tanio, Ryohei Kato, Hiroshi Noguchi, Shigetoshi Sugio, Toshiyuki Kohno, Ikuro Abe
Proceedings of the National Academy of Sciences of the United States of America 107 ( 2 ) 669 - 73 2010.1
-
Overexpression, Purification and Functional Characterization of the Human Serotonin Transporter
Hidehito Takayama, Shigetoshi Sugio
BIOPHYSICAL JOURNAL 98 ( 3 ) 505A - 505A 2010.1
-
Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6. International journal
Jiro Arima, Chiduko Sasaki, Chika Sakaguchi, Hiroshi Mizuno, Takashi Tamura, Akiko Kashima, Hitoshi Kusakabe, Shigetoshi Sugio, Kenji Inagaki
The FEBS journal 276 ( 14 ) 3894 - 903 2009.7
-
21 Structure Function Analysis of Benzalacetone Synthase(Oral Presentation)
Morita Hiroyuki, Wanibuchi Kiyofumi, Abe Tsuyoshi, Noguchi Hiroshi, Sugio Shigetoshi, Kohno Toshiyuki, Abe Ikuro
Symposium on the Chemistry of Natural Products, symposium papers 50th ( 50 ) 149 - 154 2008.9
-
Trimeric structure and conformational equilibrium of M-ficolin fibrinogen-like domain. International journal
Michikazu Tanio, Shin Kondo, Shigetoshi Sugio, Toshiyuki Kohno
Journal of synchrotron radiation 15 ( Pt 3 ) 243 - 5 2008.5
-
Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone. International journal
Hiroyuki Morita, Michikazu Tanio, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Acta crystallographica. Section F, Structural biology and crystallization communications 64 ( Pt 4 ) 304 - 6 2008.4
-
Ascaris suum cytochrome b5, an adult-specific secretory protein reducing oxygen-avid ferric hemoglobin. International journal
Muneaki Hashimoto, Shinzaburo Takamiya, Takehiro Yokota, Yoshitaka Nakajima, Fumiyuki Yamakura, Shigetoshi Sugio, Takashi Aoki
Archives of biochemistry and biophysics 471 ( 1 ) 42 - 9 2008.3
-
Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase. International journal
Hiroyuki Morita, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Acta crystallographica. Section F, Structural biology and crystallization communications 63 ( Pt 11 ) 947 - 9 2007.11
-
3 Structure-based Engineering of a Novel Plant Type III Polyketide Synthase
Morita Hiroyuki, Wanibuchi Kiyofumi, Kondo Shin, Oguro Satoshi, Noma Hisashi, Noguchi Hiroshi, Sugio Shigetoshi, Kohno Toshiyuki, Abe Ikuro
Symposium on the Chemistry of Natural Products, symposium papers 49th ( 49 ) 13 - 18 2007.8
-
Structure of the biotin carboxylase domain of pyruvate carboxylase from Bacillus thermodenitrificans. International journal
Shin Kondo, Yoshitaka Nakajima, Shigetoshi Sugio, Shinji Sueda, Md Nurul Islam, Hiroki Kondo
Acta crystallographica. Section D, Biological crystallography 63 ( Pt 8 ) 885 - 90 2007.8
-
Expression, purification and crystallization of a human tau-tubulin kinase 2 that phosphorylates tau protein. International journal
Michiko Kitano-Takahashi, Hiroyuki Morita, Shin Kondo, Kayoko Tomizawa, Ryohei Kato, Michikazu Tanio, Yoshiko Shirota, Hiroshi Takahashi, Shigetoshi Sugio, Toshiyuki Kohno
Acta crystallographica. Section F, Structural biology and crystallization communications 63 ( Pt 7 ) 602 - 4 2007.7
-
Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata. International journal
Hiroyuki Morita, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Acta crystallographica. Section F, Structural biology and crystallization communications 63 ( Pt 7 ) 576 - 8 2007.7
-
Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens. International journal
Hiroyuki Morita, Shin Kondo, Satoshi Oguro, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
Chemistry & biology 14 ( 4 ) 359 - 69 2007.4
-
Crystallization and preliminary X-ray crystallographic analysis of the receptor-uncoupled mutant of Galphai1. International journal
Tomohito Morikawa, Ayumu Muroya, Yoshitaka Nakajima, Takeshi Tanaka, Keiko Hirai, Shigetoshi Sugio, Kaori Wakamatsu, Toshiyuki Kohno
Acta crystallographica. Section F, Structural biology and crystallization communications 63 ( Pt 2 ) 139 - 41 2007.2
-
Trivalent recognition unit of innate immunity system - Crystal structure of trimeric human M-ficolin fibrinogen-like domain
Michikazu Tanio, Shin Kondo, Shigetoshi Sugio, Toshiyuki Kohno
JOURNAL OF BIOLOGICAL CHEMISTRY 282 ( 6 ) 3889 - 3895 2007.2
-
Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 angstrom resolution
Takehiro Yokota, Yukinori Nara, Akiko Kashima, Keiko Matsubara, Satoru Misawa, Ryohei Kato, Shigetoshi Sugio
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 66 ( 2 ) 272 - 278 2007.2
-
Crystallization and preliminary crystallographic analysis of a novel plant type III polyketide synthase that produces pentaketide chromone
Hiroyuki Morita, Shin Kondo, Tsuyoshi Abe, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 62 899 - 901 2006.9
-
The crystal structure of hypothetical methyltransferase from Thermus thermophilus HB8
Chizuko Sasaki, Ikuko Sugiura, Akio Ebihara, Takashi Tamura, Shigetoshi Sugio, Kenji Nagaki
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 64 ( 2 ) 552 - 557 2006.8
-
P-170 CRYSTAL STRUCTURE OF A NOVEL PLANT TYPE III POLYKETIDE SYNTHASE THAT PRODUCES PENTAKETIDE CHROMONE
Morita Hiroyuki, Kondo Shin, Oguro Satoshi, Noguchi Hiroshi, Sugio Shigetoshi, Abe Ikuro, Kohno Toshiyuki
International Symposium on the Chemistry of Natural Products 2006 "P - 170" 2006.7
-
Overexpression, purification and preliminary crystallographic analysis of human M-ficolin fibrinogen-like domain
Michikazu Tanio, Shin Kondo, Shigetoshi Sugio, Toshiyuki Kohno
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 62 652 - 655 2006.7
-
Unique structure of Ascaris suum b(5)-type cytochrome: an additional alpha-helix and positively charged residues on the surface domain interact with redox partners
T Yokota, Y Nakajima, F Yamakura, S Sugio, M Hashimoto, S Takamiya
BIOCHEMICAL JOURNAL 394 ( 2 ) 437 - 447 2006.3
-
2P037 X-ray crystallographic analysis of the receptor uncoupling mutant of Gαi1(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Morikawa Tomohito, Muroya Ayumu, Tanaka Takeshi, Sugio Shigetoshi, Wakamatsu Kaori, Kohno Toshiyuki
Seibutsu Butsuri 46 ( 2 ) S305 2006
-
2P047 Trimeric structure of human M-ficolin fibrinogen-like domain
Tanio M., Kondo S., Sugio S., Kohno T.
Seibutsu Butsuri 45 S131 2005
-
Structure of human translin at 2.2 A resolution. Reviewed International journal
Ikuko Sugiura, Chiduko Sasaki, Tsukasa Hasegawa, Toshiyuki Kohno, Shigetoshi Sugio, Hideaki Moriyama, Masataka Kasai, Takao Matsuzaki
Acta crystallographica. Section D, Biological crystallography 60 ( Pt 4 ) 674 - 9 2004.4
-
Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3 beta. Reviewed International journal
Masaaki Aoki, Takehiro Yokota, Ikuko Sugiura, Chizuko Sasaki, Tsukasa Hasegawa, Chieko Okumura, Koichi Ishiguro, Toshiyuki Kohno, Shigetoshi Sugio, Takao Matsuzaki
Acta crystallographica. Section D, Biological crystallography 60 ( Pt 3 ) 439 - 46 2004.3
-
Structure of the biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus at 2.2 Å resolution
Shin Kondo, Yoshitaka Nakajima, Shigetoshi Sugio, Yong-Biao Jin, Shinji Sueda, Hiroki Kondo
Acta Crystallographica Section D: Biological Crystallography 60 ( 3 ) 486 - 492 2004.3
-
Expression, purification and crystallization of human tau-protein kinase I/glycogen synthase kinase-3 beta Reviewed
M Aoki, M Iwamoto-Sugai, Sugiura, I, C Sasaki, T Hasegawa, C Okumura, S Sugio, T Kohno, T Matsuzaki
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 56 1464 - 1465 2000.11
-
Expression, purification and crystallization of human tau-protein kinase I/glycogen synthase kinase-3beta. International journal
M Aoki, M Iwamoto-Sugai, I Sugiura, C Sasaki, T Hasegawa, C Okumura, S Sugio, T Kohno, T Matsuzaki
Acta crystallographica. Section D, Biological crystallography 56 ( Pt 11 ) 1464 - 5 2000.11
MISC
-
Structural Characterization of L-Glutamate Oxidase from Streptomyces sp. X-119-6
Arima Jiro, Sasaki Chiduko, Sakaguchi Chika, Mizuno Hiroshi, Tamura Takashi, Kashima Akiko, Kusakabe Hitoshi, Sugio Shigetoshi, Inagaki Kenji
Vitamins 85 ( 1 ) 23 - 24 2011.1
-
結晶構造に基づく植物ポリケタイド合成酵素の反応機構の解明
森田洋行, 下川良彦, 加藤良平, 杉尾成俊, 河野俊之, 阿部郁朗
日本蛋白質科学会年会プログラム・要旨集 10th 2010
-
ダイオウ由来ベンザルアセトン合成酵素の結晶構造解析と反応機構
森田洋行, 森田洋行, 野口博司, 杉尾成俊, 河野俊之, 阿部郁朗, 阿部郁朗
天然薬物の開発と応用シンポジウム講演要旨集 17th 2008
-
ダイオウ由来ベンザルアセトン合成酵素の結晶構造解析
森田洋行, 森田洋行, 野口博司, 杉尾成俊, 河野俊之, 阿部郁朗, 阿部郁朗
日本生薬学会年会講演要旨集 55th 2008
-
M-フィコリン異物認識ドメインの三量体構造
谷生道一, 近藤伸, 杉尾成俊, 河野俊之
日本蛋白質科学会年会プログラム・要旨集 7th 2007
-
ベンタケタイドクロモン合成酵素のX線結晶構造解析
森田洋行, 近藤伸, 小黒聡史, 野口博司, 杉尾成俊, 阿部郁朗, 阿部郁朗, 河野俊之
日本薬学会年会要旨集 126th ( 4 ) 2006
-
Pronounced conversion of the metal-specific activity of superoxide dismutase from Porphyromonas gingivalis by the mutation of a single amino acid (Gly155Thr) located apart from the active site
F Yamakura, S Sugio, BY Hiraoka, D Ohmori, T Yokota
BIOCHEMISTRY 42 ( 36 ) 10790 - 10799 2003.9
-
Crystal structure of cambialistic superoxide dismutase from Porphyromonas gingivalis
S Sugio, BY Hiraoka, F Yamakura
EUROPEAN JOURNAL OF BIOCHEMISTRY 267 ( 12 ) 3487 - 3495 2000.6
Research Projects
-
Enzyme engineering for natural product biosynthesis
Grant number：23241068 2011.4 - 2015.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
ABE IKURO, WAKIMOTO Toshiyuki, AWAKAWA Takayoshi, MATSUDA Yudai, MORITA Hiroyuki, SUGIO Shigetoshi, NOGUCHI Hiroshi, KUSHIRO Testuo
Grant amount：\49400000 （ Direct Cost: \38000000 、 Indirect Cost：\11400000 ）
Several enzymes involved in the biosynthesis of natural products, such as plant polyketide synthase and CoA-ligase, exhibit remarkable catalytic versatility and substrate promiscuity. This makes the enzymes an excellent platform for enzyme engineering and further production of structurally divergent molecules. In this study, on the basis of the X-ray crystal structures of the enzymes, we succeeded in production of chemically and structurally divergent unnatural natural products, by structure-based enzyme engineering and precursor directed biosynthesis. In addition, we succeeded in elucidation of biosynthetic machinery of complex natural products including calyculin, teleocidin, and antimycin.