Updated on 2026/04/28

写真a

 
KUNISHIGE RINA
 
Organization
Institute of Integrated Research Cell Biology Center Assistant Professor
Title
Assistant Professor
External link

Degree

  • 博士（学術） ( 2021.3 )

Research Interests

  • iPS細胞

  • 神経分化

  • セミインタクト細胞

  • Targeted protein degradation

  • 画像解析

  • リシール法

  • 共焦点顕微鏡

  • 蛍光イメージング

  • ネットワーク解析

  • バイオイメージング

Research Areas

  • Life Science / Cell biology

Education

  • The University of Tokyo   Graduate School of Arts and Sciences

    2017.4 - 2021.3

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  • The University of Tokyo

    2015.4 - 2017.3

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  • The University of Tokyo   College of Arts and Sciences

    2011.4 - 2015.3

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Research History

  • Institute of Science Tokyo   Institute of Integrated Research   Specially Appointed Assistant Professor

    2025.4

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    Country：Japan

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  • Institute of Science Tokyo   Institute of Integrated Research   Specially Appointed Assistant Professor

    2024.10 - 2025.3

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  • Tokyo Institute of Technology   Institute of Innovative Research   Specially Appointed Junior Associate Professor

    2021.4 - 2024.9

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  • The University of Tokyo   Graduate School of Arts and Sciences Multi-Disciplinary Sciences Life Sciences

    2020.4 - 2021.3

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  • The University of Tokyo   Graduate School of Arts and Sciences Multi-Disciplinary Sciences Life Sciences

    2016 - 2019

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  • The University of Tokyo

    2015.4 - 2021.3

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Professional Memberships

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Papers

  • Unveiling and stratifying cell cycle-dependent drug efficacy using a single-cell PLOM-CON approach with correlation anomaly and presage protein signals

    Yoshiyuki Noguchi, Rina Kunishige, Naeko Shinozaki-Narikawa, Hiroshi Yamashita, Kazuyuki Aihara, Masayuki Murata, Fumi Kano

    Communications Biology   2025.10

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42003-025-08916-w

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  • Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targets

    Rina Kunishige, Yoshiyuki Noguchi, Naomi Okamoto, Lei Li, Akito Ono, Masayuki Murata, Fumi Kano

    Communications Biology   2025.3

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42003-025-07886-3

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  • BMP4-SMAD1/5/9-RUNX2 pathway activation inhibits neurogenesis and oligodendrogenesis in Alzheimer's patients' iPSCs in senescence-related conditions. International journal

    Daiki Nakatsu, Rina Kunishige, Yuki Taguchi, Naeko Shinozaki-Narikawa, Kishiko Osaka, Kayo Yokomizo, Mami Ishida, Shunsuke Takei, Shoko Yamasaki, Keita Hagiya, Kotaro Hattori, Tadashi Tsukamoto, Masayuki Murata, Fumi Kano

    Stem cell reports   18 ( 5 )   1246 - 1246   2023.5

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  • BMP4-SMAD1/5/9-RUNX2 pathway activation inhibits neurogenesis and oligodendrogenesis in Alzheimer's patients’ iPSCs in senescence-related conditions

    Daiki Nakatsu, Rina Kunishige, Yuki Taguchi, Naeko Shinozaki-Narikawa, Kishiko Osaka, Kayo Yokomizo, Mami Ishida, Shunsuke Takei, Shoko Yamasaki, Keita Hagiya, Kotaro Hattori, Tadashi Tsukamoto, Masayuki Murata, Fumi Kano

    Stem Cell Reports   18 ( 3 )   688 - 705   2023.3

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.stemcr.2023.01.004

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  • Targeted protein degradation by Trim-Away using cell resealing coupled with microscopic image-based quantitative analysis. International journal

    Rina Kunishige, Masayuki Murata, Fumi Kano

    Frontiers in cell and developmental biology   10   1027043 - 1027043   2022.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    "Trim-Away" technology enables rapid degradation of endogenous proteins without prior modification of protein-coding genes or mRNAs through delivery of antibodies that target proteins of interest. Although this approach can be readily applied to almost any cytosolic protein, strategies for cytosolic antibody delivery have been limited to microinjection or electroporation, which require skill-dependent operation or specialized equipment. Thus, the development of antibody delivery methods that are convenient, scalable, and preferably do not require detachment of adherent cells is required to extend the versatility of the Trim-Away method. Here, we developed a cell resealing technique optimized for Trim-Away degradation, which uses the pore-forming toxin streptolysin O (SLO) to permeabilize the cell membrane and delivered the antibodies of interest into HEK293T, HeLa, and HK-2 cell lines. We demonstrated the ability of Trim-Away protein degradation using IKKα and mTOR as targets, and we showed the availability of the developed system in antibody screening for the Trim-Away method. Furthermore, we effectively coupled Trim-Away with cyclic immunofluorescence and microscopic image-based analysis, which enables single-cell multiplexed imaging analysis. Taking advantage of this new analysis strategy, we were able to compensate for low signal-to-noise due to cell-to-cell variation, which occurs in the Trim-Away method because of the heterogenous contents of the introduced antibody, target protein, and TRIM21 in individual cells. Therefore, the reported cell resealing technique coupled with microscopic image analysis enables Trim-Away users to elucidate target protein function and the effects of target protein degradation on various cellular functions in a more quantitative and precise manner.

    DOI： 10.3389/fcell.2022.1027043

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  • Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling

    Yoshiyuki Noguchi, Fumi Kano, Nobuhiko Maiya, Chisako Iwamoto, Shoko Yamasaki, Yosuke Otsubo, Daiki Nakatsu, Rina Kunishige, Masayuki Murata

    iScience   24 ( 7 )   102724 - 102724   2021.7

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.isci.2021.102724

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  • Calcium phosphate microcrystals in the renal tubular fluid accelerate chronic kidney disease progression

    Kazuhiro Shiizaki, Asako Tsubouchi, Yutaka Miura, Kinya Seo, Takahiro Kuchimaru, Hirosaka Hayashi, Yoshitaka Iwazu, Marina Miura, Batpurev Battulga, Nobuhiko Ohno, Toru Hara, Rina Kunishige, Mamiko Masutani, Keita Negishi, Kazuomi Kario, Kazuhiko Kotani, Toshiyuki Yamada, Daisuke Nagata, Issei Komuro, Hiroshi Itoh, Hiroshi Kurosu, Masayuki Murata, Makoto Kuro-o

    The Journal of Clinical Investigation   2021.6

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    Publishing type：Research paper (scientific journal)   Publisher：The American Society for Clinical Investigation  

    DOI： 10.1172/JCI145693

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  • Calciprotein particle-induced cytotoxicity via lysosomal dysfunction and altered cholesterol distribution in renal epithelial HK-2 cells

    Rina Kunishige, Mai Mizoguchi, Asako Tsubouchi, Kenjiro Hanaoka, Yutaka Miura, Hiroshi Kurosu, Yasuteru Urano, Makoto Kuro-o, Masayuki Murata

    Scientific Reports   10 ( 1 )   2020.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media {LLC}  

    <jats:title>Abstract</jats:title><jats:p>Dietary phosphate overload induces chronic kidney disease (CKD), and calciprotein particles (CPPs), a form of nanoparticle comprising calcium phosphate and serum proteins, has been proposed to cause renal toxicity. However, the mechanism of CPP cytotoxicity in renal tubular cells is unknown. Here we show that in renal proximal tubular epithelial HK-2 cells, endocytosed CPPs accumulate in late endosomes/lysosomes (LELs) and increase their luminal pH by ~ 1.0 unit. This results in a decrease in lysosomal hydrolase activity and autophagic flux blockage without lysosomal rupture and reactive oxygen species generation. CPP treatment led to vulnerability to H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>-induced oxidative stress and plasma membrane injury, probably because of autophagic flux blockage and decreased plasma membrane cholesterol, respectively. CPP-induced disruption of lysosomal homeostasis, autophagy flux and plasma membrane integrity might trigger a vicious cycle, leading to progressive nephron loss.</jats:p>

    DOI： 10.1038/s41598-020-77308-3

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    Other Link： http://www.nature.com/articles/s41598-020-77308-3

  • The cell resealing technique for manipulating, visualizing, and elucidating molecular functions in living cells

    Rina Kunishige, Fumi Kano, Masayuki Murata

    Biochimica et Biophysica Acta (BBA) - General Subjects   1864 ( 2 )   129329 - 129329   2020.2

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.bbagen.2019.03.015

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  • Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Brucella Infection

    Yuki Taguchi, Koichi Imaoka, Michiyo Kataoka, Akihiko Uda, Daiki Nakatsu, Sakuya Horii-Okazaki, Rina Kunishige, Fumi Kano, Masayuki Murata

    PLOS Pathogens   11 ( 3 )   2015.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science ({PLoS})  

    DOI： 10.1371/journal.ppat.1004747

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Books

  • 革新的AI創薬 : 医療ビッグデータ、人工知能がもたらす創薬研究の未来像

    小長谷, 明彦（ Role： Contributor第6節 共変動ネットワーク解析法とリシール細胞技術を基盤とするAI主導の創薬・細胞医薬支援プラットフォーム創成）

    エヌ・ティー・エス  2022.7  （ ISBN:9784860437886 ）

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    Total pages：2, 7, 350, 8p, 図版15p   Language：Japanese  

    CiNii Books

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MISC

  • Development of signal pathway analysis method utilizing intracellular localization information of protein

    Nobuhiko Maiya, Yoshiyuki Noguchi, Fumi Kano, Chisako Iwamoto, Shoko Yamasaki, Yosuke Otsubo, Daiki Nakatsu, Rina Kunishige, Masayuki Murata

    2022.7

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    Publishing type：Research paper, summary (national, other academic conference)  

    DOI： 10.14869/toxpt.49.1.0_O-39

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  • セミインタクト細胞リシール技術を用いた新しい細胞編集・細胞デザイン

    加納 ふみ, 國重 莉奈

    細胞   54 ( 4 )   186 - 189   2022.4

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：(株)ニュー・サイエンス社  

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  • セミインタクト細胞リシール技術を用いた細胞機能解析

    國重 莉奈, 加納 ふみ, 村田 昌之

    生体の科学   69 ( 3 )   247 - 251   2018.6

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.11477/mf.2425200804

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Presentations

  • 細胞状態の変化を活写するネットワーク解析法を用いた新規フェロトーシス誘導剤の作用機序解明

    國重 莉奈, 野口 誉之, 岡本 直美, 李 蕾, 尾野 晃人, 村田 昌之, 加納 ふみ

    第98回日本生化学会大会 

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    Event date： 2025.11

    Presentation type：Poster presentation  

    Venue：国立京都国際会館  

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  • Analyzing cellular senescence based on organelle structural and functional changes

    Noriko Yano, Akira Mitsuishi, Rina Kunishige, Masayuki Murata, Fumi Kano

    Joint Meeting of the 77th JSCB and 58th JSDB 

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    Event date： 2025.7

    Presentation type：Poster presentation  

    Venue：WINC AICHI  

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  • Elucidating ferroptosis inducer mechanisms and potential synergistic drug targets: application of PLOM-CON analysis

    Rina Kunishige, Yoshiyuki Noguchi, Naomi Okamoto, Lei Li, Akito Ono, Masayuki Murata, Fumi Kano

    Joint Meeting of the 77th JSCB and 58th JSDB 

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    Event date： 2025.7

    Presentation type：Poster presentation  

    Venue：WINC AICHI  

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  • 蛋白質の細胞内局在情報を活用したシグナル伝達系分析手法の開発と抗がん剤の作用機序研究への応用

    國重 莉奈, 野口 誉之, 田口 由起, 仲矢 丈雄, 相澤 健一, 米谷 信彦, 菅原 稔, 永井 良三, 村田 昌之, 加納 ふみ

    日本薬学会第145年会 

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    Event date： 2025.3

    Presentation type：Poster presentation  

    Venue：福岡国際会議場・マリンメッセ福岡  

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  • マルチプレックスIF法を用いたオルガネラ同士の量的調節機構の解明

    井上 慶彦, 成川 苗子, 國重 莉奈, 加納 ふみ

    日本分子生物学会 第47回 年会  2024.11 

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    Event date： 2024.11

    Presentation type：Poster presentation  

    Venue：福岡国際会議場・マリンメッセ福岡  

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  • SMADタンパク質は白色脂肪細胞のベージュ化を調節する

    関根 涼太, 田口 由起, 中津 大貴, 國重 莉奈, 加納 ふみ

    日本分子生物学会 第47回 年会  2024.11 

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    Event date： 2024.11

    Presentation type：Poster presentation  

    Venue：福岡国際会議場・マリンメッセ福岡  

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  • Cell Editing Using the Semi-Intact Cell Resealing Technique: Towards Enhanced Biology Invited

    Rina Kunishige, Masayuki Murata, Fumi Kano

    MBSJ2024  2024.11  The Molecular Biology Society of Japan

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    Event date： 2024.11

    Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

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  • 蛋白質の細胞内局在情報を活用したシグナル伝達経路分析手法の開発

    國重 莉奈, 野口 誉之, 田口 由起, 仲矢 丈雄, 相澤 健一, 米谷 信彦, 菅原 稔, 永井 良三, 村田 昌之, 加納 ふみ

    第９７回日本生化学会大会 

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    Event date： 2024.11

    Presentation type：Poster presentation  

    Venue：パシフィコ横浜ノース  

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  • 細胞をデザインする

    國重 莉奈

    すずかけサイエンスデイ2024  2024.5  東京工業大学

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    Event date： 2024.5

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：東京工業大学 すずかけ台キャンパス   Country：Japan  

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  • 細胞応答・細胞状態を表すタンパク質共変動ネットワーク: アクチンドメインの機能解析例

    國重 莉奈, 野口 誉之, 米谷 信彦, 中津 大貴, 村田 昌之, 加納 ふみ

    第15回「光塾」  2024.1 

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    Event date： 2024.1

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 細胞応答・細胞状態を表すタンパク質共変動ネットワーク

    國重 莉奈

    定量生物学の会 第十一回年会  2024.1 

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    Event date： 2024.1

    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京大学 駒場IIキャンパス 生産技術研究所   Country：Japan  

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  • Protein co-variation network analysis aimed at analyzing stratified cellular responses

    Rina Kunishige, Yoshiyuki Noguchi, Nobuhiko Maiya, Daiki Nakatsu, Masayuki Murata, Fumi Kano

    2023.12 

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    Event date： 2023.12

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

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  • リシール細胞技術を用いた細胞内抗体導入によるTrim-Away標的タンパク質分解とマルチプレックス蛍光抗体法を組み合わせたタンパク質機能解析系の構築

    國重 莉奈, 村田 昌之, 加納 ふみ

    第96回日本生化学会大会  2023.10 

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    Event date： 2023.10 - 2023.11

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 患者由来 iPS 細胞を用いたアルツハイマー依存的神経新生不全モデルの構築と構築モデル を用いた神経新生不全回復薬の同定

    中津 大貴, 國重 莉奈, 田口 由起, 成川-篠崎 苗子, 大坂 企志子, 横溝 佳代, 石田 真美, 武居 俊輔, 山﨑 聖子, 萩屋 啓太, 服部 功太郎, 塚本 忠, 村田 昌之, 加納 ふみ

    第75回日本細胞生物学会大会  2023.6 

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    Event date： 2023.6

    Presentation type：Poster presentation  

    Venue：奈良県コンベンションセンター  

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  • Detecting Immediate Cellular Responses to Drugs with PLOM-CON Analysis: A Network-based Approach

    Rina Kunishige, Yoshiyuki Noguchi, Nobuhiko Maiya, Daiki Nakatsu, Masayuki Murata, Fumi Kano

    2023.6 

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    Event date： 2023.6

    Language：English   Presentation type：Poster presentation  

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  • Cell resealing-mediated targeted protein degradation by Trim-Away coupled with microscopic image-based quantitative analysis

    Rina Kunishige, Masayuki Murata, Fumi Kano

    2023.5 

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    Event date： 2023.5

    Presentation type：Poster presentation  

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  • Transient formation of actin domain mediates insulin-induced glycogen synthesis: application of a novel Covariation Network analysis

    Rina Kunishige, Yoshiyuki Noguchi, Nobuhiko Maiya, Daiki Nakatsu, Masayuki Murata, Fumi Kano

    2022.11 

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    Event date： 2022.11

    Presentation type：Oral presentation (general)  

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  • リシール細胞技術を用いた細胞内環境改変法と共変動ネットワークを用いたフェノタイプ解析 Invited

    加納 ふみ, 國重 莉奈

    第95回日本生化学会大会  2022.11 

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    Event date： 2022.11

    Venue：名古屋国際会議場  

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  • Development of signal pathway analysis method utilizing intracellular localization information of protein

    Nobuhiko Maiya, Yoshiyuki Noguchi, Fumi Kano, Chisako Iwamoto, Shoko Yamasaki, Yosuke Otsubo, Daiki Nakatsu, Rina Kunishige, Masayuki Murata

    2022.7 

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    Event date： 2022.6 - 2022.7

    Presentation type：Oral presentation (general)  

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  • PLOM-CON 解析: 細胞形態およびタンパク質の修飾状態を考慮した細胞内状態の時系列変化の新規推定法

    野口 誉之, 國重 莉奈, 米谷 信彦, 中津 大貴, 村田 昌之, 加納 ふみ

    第74回日本細胞生物学会大会要旨集  2022.6 

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    Event date： 2022.6

    Presentation type：Poster presentation  

    Venue：タワーホール船堀  

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  • Transient formation of actin domain mediates insulin signaling: application of PLOM-CON analysis

    Rina Kunishige, Yoshiyuki Noguchi, Nobuhiko Maiya, Daiki Nakatsu, Masayuki Murata, Fumi Kano

    2022.6 

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    Event date： 2022.6

    Presentation type：Poster presentation  

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  • 蛋白質の細胞内局在情報を活用したシグナル伝達経路分析手法の開発

    野口 誉之, 加納 ふみ, 米谷 信彦, 岩本 智沙子, 山﨑 聖子, 大坪 洋介, 中津 大貴, 國重 莉奈, 村田 昌之

    日本薬学会第142年会  2022.3 

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    Event date： 2022.3

    Presentation type：Poster presentation  

    Venue：オンライン開催  

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  • オルガネラ形態変化の再構成と分子機序解明を可能にするリシール細胞技術と共変動ネットワーク解析法 Invited

    加納ふみ, 國重莉奈

    第44回分子生物学会年会  2021.12 

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    Event date： 2021.12

    Presentation type：Symposium, workshop panel (public)  

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  • Functional Analysis and Phenotyping of the Cells created by Cell-Resealing Technique

    Rina Kunishige, Yuki Sonoda, Masataka Murakami, Fumi Kano, Masayuki Murata

    2019.6 

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    Presentation type：Poster presentation  

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  • 「細胞状態」の時空間的変化を活写する共変動ネットワーク解析法による薬効評価

    國重 莉奈, 野口 誉之, 米谷 信彦, 中津 大貴, 村田 昌之, 加納 ふみ

    第96回日本薬理学会年会  2022.12 

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    Presentation type：Poster presentation  

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  • シグナル伝達系タンパク質の時空間ダイナミクスを捉える新規ネットワーク解析法の開発と薬効評価への応用

    國重 莉奈, 野口 誉之, 米谷 信彦, 中津 大貴, 村田 昌之, 加納 ふみ

    第147回日本薬理学会関東部会  2023.3 

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    Presentation type：Poster presentation  

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Industrial property rights

  • リシール細胞セット及びその製造方法

    加納ふみ, 村田昌之, 國重莉奈, 野口誉之

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    Applicant：東京工業大学・東京大学

    Application no：特願2022-165180  Date applied：2022.10

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  • 解析システム、解析装置、解析プログラム、及び解析方法

    村田 昌之, 加納 ふみ, 國重 莉奈, 野口 誉之

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    Applicant：東京工業大学・東京大学

    Application no：特願2022-111995  Date applied：2022.7

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Awards

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