Organization
Institute of Integrated Research Laboratory for Chemistry and Life Science Assistant Professor
Title
Assistant Professor
Contact information
External link
ZHU BO
News & Topics
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Quenchbody Immunosensors Pave the Way to Quick and Sensitive COVID-19 Diagnostics
2022/12/15
Languages： English
A new immunosensor based on Quenchbody technology shows great potential as a fast, inexpensive, and convenient tool to detect SARS-CoV-2. Developed by scientists at Tokyo Institute of Technology
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臨床検体中のコロナウイルスタンパク質を蛍光抗体で迅速定量することに成功 蛍光検出の速度向上法としても期待
2022/12/15
Languages： Japanese
要点 新型コロナウイルス抗原に結合すると光る抗体断片Q-bodyを構築し、抗原の簡便な検出に成功 反応液に高分子を加えることで反応を加速し、検出感度が向上 臨床サンプル中の新型コロナウイルス検出が確認でき、感染の有無を判定 概要東京工業大学 科学技術創成研究院 化学生命科学研究所の朱博助教と上田宏教授、東京医科歯科大学 大学院医歯学総合研究科 生体集中管理学分野の野坂宜之講師と若林健二教
Degree
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Ph.D. (Agriculture) ( 2016.3 Nagoya University )
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Master (Agriculture) ( 2013.3 Nagoya University )
Research Interests
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バイオ医薬品
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タンパク質言語モデル
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抗体工学
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合成生物学
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酵素工学
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バイオセンサー
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高速分子進化
Research Areas
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Nanotechnology/Materials / Chemical biology
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Life Science / Biomedical engineering
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Life Science / Applied microbiology
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
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Life Science / Applied biochemistry
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Life Science / Molecular biology
Research History
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Institute of Science Tokyo Laboratory for Chemistry and Life Science, Institute of Integrated Research Assistant Professor
2024.10
Country：Japan
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National Yang Ming Chiao Tung University Guest Lecturer
2023.12
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University of California, Los Angeles Visiting scholar
2023.10 - 2023.11
Country：United States
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HikariQ Health R&D Manager
2023.3
Country：Japan
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Tokyo Institute of Technology Laboratory for Chemistry and Life Science, Institute of Innovative Research Assistant Professor
2020.5 - 2024.9
Country：Japan
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Kobe University Graduate School of Science, Technology and Innovation Postdoctoral Associate
2018.7 - 2020.4
Country：United States
Professional Memberships
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日本蛋白質科学会
2024.1
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日本抗体学会
2023.5
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日本生物工学会
2022.10
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日本化学工学会
2020.12
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日本酵素工学研究会
2020.10
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生物化学的測定研究会
2020.10
Committee Memberships
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3rdBottom-up Biotech Hands-on Workshop Organizer
2025.6 - 2025.8
Committee type：Other
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日本生物工学会東日本支部2025年コロキウム オーガナイザー
2024.11 - 2025.3
Committee type：Academic society
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第76回日本生物工学会大会 大会実行委員
2024.7 - 2024.9
Committee type：Academic society
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生物工学若手研究者の集い（若手会） 夏のセミナー実行委員
2021.11 - 2023.6
Committee type：Academic society
Papers
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Zhirou Qiu, Bo Zhu, Takanobu Yasuda, Hiroshi Ueda, Tetsuya Kitaguchi
ACS Omega in press 2025.6
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SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase
Kalhari Munaweera, Nana Odake, Kakeru Ikeda, Bo Zhu, Maurizio Camagna, Tomokazu Ito, Tetsuya Kitaguchi, Naoto Nemoto, Hideo Nakano, Jasmina Damnjanović
2025.3
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Akihito Inoue, Bo Zhu, Keisuke Mizutani, Ken Kobayashi, Takanobu Yasuda, Alon Wellner, Chang C. Liu, Tetsuya Kitaguchi
JACS Au 5 ( 2 ) 955 - 964 2025.2
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Comprehensive Protease Specificity Profiling
Bo Zhu, Michael D. Lane, Joshua A. Baller, Pratik D. Jagtap, Timothy J. Griffin, Burckhard Seelig
bioRxiv 2024.11.06.622033 2024.11
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BRET Nano Q-Body: A Nanobody-Based Ratiometric Bioluminescent Immunosensor for Point-of-Care Testing Reviewed
Yinghui Yang, Akihito Inoue, Takanobu Yasuda, Hiroshi Ueda, Bo Zhu, Tetsuya Kitaguchi
ACS Sensors 9 ( 11 ) 5955 - 5965 2024.11
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Customizable OpenGUS immunoassay: a homogeneous detection system using β-glucuronidase switch and label-free antibody Reviewed
Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Mitsue Nagamine, Hiroshi Ueda, Tetsuya Kitaguchi
Biosensors and Bioelectronics 267 116796 2024.9
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Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets Reviewed
Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitguchi, Sebastian Behrens, Burckhard Seelig
Biosensors 13 ( 8 ) 768 2023.7
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Satoshi Okada, Yuka Muto, Bo Zhu, Hiroshi Ueda, Hiroyuki Nakamura
Analytical Chemistry 95 ( 15 ) 6198 - 6202 2023.4
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Abdul Qawee Rani, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi
Analyst 148 1422 - 1429 2023.2
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Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda
Biochemistry 62 ( 2 ) 309 - 317 2023.1
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The Patrol Yeast: A new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning Reviewed
Jiulong Su, Bo Zhu, Akihito Inoue, Hiroyuki Oyama, Izumi Morita, Jinhua Dong, Takanobu Yasuda, Yoshiko Sugita-Konishi, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda
Biosensors and Bioelectronics 219 114793 - 114793 2023.1
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Isolation of a Human SARS-CoV-2 Neutralizing Antibody from a Synthetic Phage Library and its Conversion to Fluorescent Biosensors Reviewed
Haimei Li, Bo Zhu, Baowei Li, Limei Chen, Xuerao Ning, Hang Dong, Jingru Liang, Xueying Yang, Jinhua Dong, Hiroshi Ueda
Scientific Reports 12 ( 1 ) 15496 2022.9
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Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents Reviewed International journal
Bo Zhu, Nobuyuki Nosaka, Shuji Kanamaru, Jinhua Dong, Yancen Dai, Akihito Inoue, Yinghui Yang, Kaori Kobayashi, Tetsuya Kitaguchi, Hiroshi Iwasaki, Ryuji Koike, Kenji Wakabayashi, Hiroshi Ueda
Analyst 147 ( 22 ) 4971 - 4979 2022.9
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Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimura, Farid J. Ghadessy, Hiroshi Ueda
Chemical Science 13 ( 33 ) 9739 - 9748 2022.8
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Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface Reviewed
Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami, Hiroshi Ueda
Scientific Reports 11 ( 1 ) 22590 2021.11
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Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins Reviewed International journal
Bo Zhu, Rui Gan, Maria D. Cabezas, Takaaki Kojima, Robert Nicol, Michael C. Jewet, Hideo Nakano
Biotechnology and Bioengineering 117 ( 12 ) 3849 - 3857 2020.9
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Yi Xiao, Zhe Du, Yuqian Li, Lijia Cao, Bo Zhu, Tetsuya Kitaguchi, Caihong Huang
Sensors 25 ( 16 ) 4945 - 4945 2025.8
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Development of bioluminescent Switchbody, antigen-triggered enzyme switch and elucidation of its principle
Takanobu Yasuda, Yoshiyuki Ueno, Masahiko Taguchi, Naoya Tochio, Hiromasa Yagi, Shuma Yazaki, Ryoichi Arai, Bo Zhu, Takanori Kigawa, Hiroshi Ueda, Tetsuya Kitaguchi
Jxiv Preprint https://doi.org/10.51094/jxiv.1096 2025.2
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Detection of a large antigen through the masking and exposure of a fragment of split luciferase Reviewed
Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi
Analytical Sciences 41 ( 6 ) 857 - 865 2025
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Kana Sasamoto, Takanobu Yasuda, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi
Analyst 148 5843 - 5850 2023.10
Books
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切らずに開く酵素スイッチ
朱 博, 安田 貴信, 北口 哲也
日本化学会バイオテクノロジー部 ニュースレターVol. 27, No. 2 (2024. 03. 27)
Presentations
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Rapid development of single fluorescent protein-based immunosensor Flashbody through ultra-high-throughput screening and cell-free protein synthesis
Bo Zhu, Zhirou Qiu, Keisuke Mizutani, Ken Kobayashi, Hiroshi Ueda, Tetsuya Kitaguchi
The 75th Annual Meeting of the Society Biotechnology, Japan 2023.9
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Quenchbody-based 1-minute detection assay for SARS-CoV-2 and variants of concern
2021.9
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Development of quenchbody-based 1-minute SARS-CoV-2 detection assay
2021.3
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Development of diverse immunosensors through rapid molecular evolutions and deep learning Invited
Bo Zhu
2024.2
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Molecular and whole-cell immunosensors developed by directed evolution for applications in diagnostics and food-safety control Invited
Bo Zhu
Biotechnology Institute Seminar 2023 Fall, University of Minnesota 2023.11
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Converting antibodies to rapid response SARS-CoV-2 homogeneous immunosensors via Quenchbody technology and crowding agent
2022.9
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Rapid construction of homogeneous immunosensors for SARS-CoV-2 nucleocapsid and spike proteins using Quenchbody and Quenchprobe technology
Bo Zhu, Haimei Li, Yancen Dai, Akihito Inoue, Yinghui Yang, Kaori Kobayashi, Tetsuya Kitaguchi, Jinhua Dong, Hiroshi Ueda
Bioanalytical Sensors Gordon Research Conference 2022.6
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Interaction kinetics analysis of a three-split luciferase-based open sandwich homogeneous immunoassay for mathematical model generation
Bo Zhu, Rianto Sato, Lauren Rogers, Abbie Bui, Takanobu Yasuda, Hiroshi Ueda, Naohiro Kato, Tetsuya Kitaguchi
The 76th Annual Meeting of the Society of Biotechnology of Japan 2024.9
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A one-pot detection system using β-glucuronidase-based enzyme switch and label-free antibody
Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Hiroshi Ueda, Tetsuya Kitaguchi
International Union of Pure and Applied Biophysics (IUPAB) 2024 2024.6
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A customizable one-pot detection system using β-glucuronidase switch and label-free antibody
Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Hiroshi Ueda, Tetsuya Kitaguchi
SCEJ 89th Annual Meeting 2024.3
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Sequence-to-function deep learning framework for fluorescent immunosensor engineering
Bo Zhu, Akihito Inoue, Keisuke Mizutani, Ken Kobayashi, Takanobu Yasuda, Tetsuya Kitaguchi
2025.9
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Ultrahigh-throughput screening × AI for Biomolecular Engineering Invited
Bo Zhu
2025.6
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出芽酵母における翻訳促進ペプチドタグに関する研究
岡田航輝, 間部悟司, 加藤晃代, 朱博, 北口哲也, 中野秀雄
日本農芸化学会 中部・関西支部合同大会 2023.10
Industrial property rights
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細胞質多角体タンパク質と標的タンパク質とを含む複合体及びその製造方法
上野隆史, リョウ シシン, 菊池幸祐, 北口哲也, 朱博
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光免疫療法の副作用を低減する光増感剤消光剤修飾抗体
北口 哲也, 朱 博, 安田 貴信, キュウ シジュウ
Awards
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末松賞「革新的価値創造の基礎と展開」
2025.8 東京科学大学 超安定分⼦ディスプレイ法の創成
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第34回 生物工学技術賞
2025.5 日本生物工学会 スイッチ酵素を利用した汎用的な均一系免疫測定法の開発とその商品化
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Takeda-rika Award for Young Researcher
2025.1
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第1回社会変革チャレンジ賞
2024.6 東工大イノベーションデザイン機構（Id機構） 薬物放出制御のためのスイッチ型抗体薬物複合体の創出
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On-chip Biotechnologies Award
2023.10
Bo Zhu
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SBJ East Japan Branch Director Award
2023.7 SBJ East Japan Branch Development of diverse immunosensors through the integration of rapid molecular evolution and in silico structural analysis
Bo Zhu
Research Projects
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Analysis of the structural dynamics of various proteins with switching functions
2025.5 - 2026.3
九州大学生体防御医学研究所 「多階層生体防御システム研究拠点」 共同研究費
朱博, 渡部 聡
Authorship：Principal investigator
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革新的薬物放出制御を実現するスイッチ型抗体薬物複合体の創出
2025.4 - 2027.3
中谷財団 2025（令和7）年度奨励研究助成
朱博
Authorship：Principal investigator
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Protein engineering of D-amino acid oxidase and transglutaminase using novel single-molecule display technology coupled with Nanopore sequencing
Grant number：20251233 2025.4 - 2026.3
Materials and Devices Joint Research Center Basic Collaborative Research
Damnjanovic Jasmina, Zhu Bo, Kitaguchi Tetsuya, Nakano Hideo
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向精神薬と代謝物に対するin silico解析に基づく戦略的抗体獲得と検出法開発
Grant number：24K02719 2024.4 - 2028.3
日本学術振興会 科学研究費助成事業 基盤研究(B)
笹尾 亜子, 朱 博, 安田 貴信, 笹尾 明
Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）
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機械学習を活用したユニバーサルな基盤構造創出による蛍光免疫センサーの迅速開発
Grant number：24K01264 2024.4 - 2027.3
日本学術振興会 科学研究費助成事業 基盤研究(B)
北口 哲也, 朱 博, 坪井 貴司, 鈴木 団
Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）
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Creatation of antiboy-peptide-nucleic acid conjugates with switch function and its application in a high-sensitive homogeneous detection system
2024.4 - 2026.3
Grant-in-Aid for Transformative Research Areas（A）（Publicly Offered Research）
Bo Zhu
Authorship：Principal investigator
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AI 支援と構造解析を融合したスイッチ型抗体薬物複合体の超高速スクリーニング法の創成
2024.4 - 2025.3
⼈と知と物質で未来を創るクロスオーバーアライアンス 若手フィージビリティスタディ共同研究
朱 博, 田畑 公次, 高田 悠里, 田口 真彦, 北口 哲也
Authorship：Principal investigator
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Exploration of microdroplet-based screening methods for rapid engineering of biosensor, bacteria, and functional protein
2024.4
Bo Zhu
Authorship：Principal investigator
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将来のパンデミックに備えた迅速対応基盤
2023.10 - 2025.3
東京工業大学 科学技術創成研究院 プレ研究ユニット制度
朱博
Authorship：Principal investigator
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Universal Bio and AI tools for high-throughput engineering of functional proteins
2023.9 - 2024.3
Bo Zhu, Ken Kobayashi
Authorship：Principal investigator
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Development of hyperstable homogeneous biosensors for virus and cytokine detections
2023.8 - 2024.3
Bo Zhu, Nobuyuki Nosaka, Tetsuya Kitaguchi
Authorship：Principal investigator
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AI 支援と構造解析を融合したスイッチ型抗体薬物複合体の超高速スクリーニング法の創成
2023.4 - 2024.3
⼈と知と物質で創るクロスオーバーアライアンス 若手フィージビリティスタディ共同研究
朱博, 田畑 公次, 渡部 聡, 北口 哲也, Yinghui Yang
Authorship：Principal investigator
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Creating biological and AI tools for high-throughput development of biosensors
2022.12 - 2023.3
Bo Zhu, Ken Kobayashi
Authorship：Principal investigator
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Better understanding of a fluorescent immunosensor via ultra-high-throughput screening
2021.11 - 2022.3
Bo Zhu
Authorship：Principal investigator
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Development of a generalizable toolkit for rapidly improving the sensitivity of fluorescent immunosensor proteins
Grant number：21K14468 2021.4 - 2024.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists Grant-in-Aid for Early-Career Scientists
Bo Zhu
Authorship：Principal investigator
Grant amount：\3510000 （ Direct Cost: \2700000 、 Indirect Cost：\810000 ）
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Rational design of biosensor based on computational science and biochemical experiment
Grant number：2025Y010 2025.4 - 2027.3
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薬物スクリーニングに適した催眠剤ザレプロンに対するQuenchbody作製
Grant number：20251205 2025.4 - 2026.3
物質・デバイス領域共同研究拠点 基盤共同研究
平田香子, 安田貴信, 朱博, 北口哲也
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Development of single-molecule display technology for directed evolution of D-amino acid oxidase with high substrate specificity
Grant number：20241220 2024.4 - 2025.3
Materials and Devices Joint Research Center Basic Collaborative Research
Damnjanovic Jasmina, Zhu Bo, Kitaguchi Tetsuya, Nakano Hideo
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Development of single molecule display technology for directed evolution of proteins
Grant number：20231204 2023.4 - 2024.3
Materials and Devices Joint Research Center Basic Collaborative Research
Damnjanovic Jasmina, Zhu Bo, Kitaguchi Tetsuya, Nakano Hideo
Teaching Experience
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Scientific discussions in English
2025 Institution：Institute of Science Tokyo
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Biochemistry discussions in English
2021 - 2023 Institution：Tokyo Institute of Technology
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LST Basic Laboratory and Exercise IV
2020 Institution：Tokyo Institute of Technology
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Biology Saves the World project
2018 Institution：University of Minnesota
Media Coverage
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東京科学大学 プレスリリース Science Tokyoニュース 2025.4
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牛乳や血液をそのまま薄めず標的分子を検出できる免疫センサーを開発 Internet
東京科学大学 プレスリリース Science Tokyoニュース 2024.11
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抗体と混ぜるだけで洗浄不要の免疫測定法を実現する新たな測定素子を開発
東京科学大学 Science Tokyoニュース 2024.10
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Quenchbody Immunosensors Pave the Way to Quick and Sensitive COVID-19 Diagnostics Internet
2022.12
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Quenchbody immunosensors pave the way to quick and sensitive COVID-19 diagnostics Internet
American Association for the Advancement of Science (AAAS) EurekAlert https://www.eurekalert.org/news-releases/974536 2022.12
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臨床検体中のコロナウイルスタンパク質を蛍光抗体で迅速定量することに成功
東京医科歯科大学プレスリリース https://www.tmd.ac.jp/press-release/202221212-1/ 2022.12