Hic-5 antisense oligonucleotide inhibits advanced hepatic fibrosis and steatosis in vivo

Masahito Noguchi, Aya Miyauchi, Yoshiaki Masaki, Masashi Sakaki, Xiao-Feng Lei, Momoko Kobayashi-Tanabe, Akira Miyazaki, Takeshi Aoki, Hitoshi Yoshida, Kohji Seio, Joo-ri Kim-Kaneyama

JHEP REPORTS   6 ( 11 )   2024年11月

Inhibitory Effects on RNA Binding and RNase H Induction Activity of Prodrug-Type Oligodeoxynucleotides Modified with a Galactosylated Self-Immolative Linker Cleavable by β-Galactosidase

Kento Miyaji, Yoshiaki Masaki, Kohji Seio

BIOCONJUGATE CHEMISTRY   35 ( 10 )   1587 - 1607   2024年10月

Synthesis and Conformational Analyses of Cyclonucleoside Having 13-Membered Ring Bridging Nucleobase and 5′-Position via a Linker Containing Sulfonamide

Takayuki Kanagawa, Shigetoshi Tachibana, Yoshiaki Masaki, Kohji Seio

ORGANIC LETTERS   25 ( 43 )   7868 - 7872   2023年10月

Alteration of target cleavage patterns and off-target reduction of antisense oligonucleotides incorporating 2-N-carbamoyl- or (2-pyridyl)guanine 国際誌

Takayuki Kanagawa, Aya Koyoma, Yoshiaki Masaki, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   21 ( 25 )   5214 - 5224   2023年6月

Insertion of a methylene group into the backbone of an antisense oligonucleotide reveals the importance of deoxyribose recognition by RNase H

Yoshiaki Masaki, Ayano Tabira, Shihori Hattori, Shunsuke Wakatsuki, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   20 ( 45 )   8917 - 8924   2022年11月

Synthesis of 2′-O-[3-(N-methylsulfamoyl)propan-1-yl]ribothymidine as a potentially applicable 2′-modified nucleoside for antisense oligonucleotides

Takahito Tomori, Koya Uekusa, Aya Koyama, Takayuki Kanagawa, Yoshiaki Masaki, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   73   2022年11月

Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides

Yoshiaki Masaki, Yukiko Onishi, Kohji Seio

SCIENTIFIC REPORTS   12 ( 1 )   2022年7月

Selective and stable base pairing by alkynylated nucleosides featuring a spatially-separated recognition interface

Hidenori Okamura, Giang Hoang Trinh, Zhuoxin Dong, Yoshiaki Masaki, Kohji Seio, Fumi Nagatsugi

NUCLEIC ACIDS RESEARCH   50 ( 6 )   3042 - 3055   2022年4月

Oligodeoxynucleotides Modified with 2′-O-(Cysteinylaminobutyl)carbamoylethylribothymidine Residues for Native Chemical Ligation with Peptide at Internal Positions

Yoshiaki Masaki, Atsuya Maruyama, Keita Yoshida, Takahito Tomori, Tomohiro Kishimura, Kohji Seio

BIOCONJUGATE CHEMISTRY   33 ( 2 )   272 - 278   2022年2月

Synthesis of 2′-O-alkylcarbamoylethyl-modified oligonucleotides with enhanced nuclease resistance that form isostable duplexes with complementary RNA

Tomohiro Kishimura, Takahito Tomori, Yoshiaki Masaki, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   35   2021年3月

Novel EGFP reporter cell and mouse models for sensitive imaging and quantification of exon skipping

Yuko Hara, Yoshitaka Mizobe, Yukiko U. Inoue, Yasumasa Hashimoto, Norio Motohashi, Yoshiaki Masaki, Kohji Seio, Shin'ichi Takeda, Tetsuya Nagata, Matthew J. A. Wood, Takayoshi Inoue, Yoshitsugu Aoki

SCIENTIFIC REPORTS   10 ( 1 )   2020年6月

Synthesis of Deoxypseudouridine 5′-Triphosphate Bearing the Photoremovable Protecting Group at the N1 Position Capable of Enzymatic Incorporation to DNA

Leo Takeshita, Yuji Yamada, Yoshiaki Masaki, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   85 ( 4 )   1861 - 1870   2020年2月

Transcription of DNA duplex containing deoxypseudouridine and deoxypseudoisocytidine, and inhibition of transcription by triplex forming oligonucleotide that recognizes the modified duplex. 国際誌

Kohji Seio, Kei Yamaguchi, Ayano Yamazaki, Takashi Kanamori, Yoshiaki Masaki

Nucleosides, nucleotides & nucleic acids   39 ( 6 )   892 - 904   2020年

Crystal structure of a DNA duplex cross-linked by 6-thioguanine-6-thioguanine disulfides: reversible formation and cleavage catalyzed by Cu(ii) ions and glutathione

Akira Ono, Takahiro Atsugi, Misato Goto, Hisao Saneyoshi, Takahito Tomori, Kohji Seio, Takenori Dairaku, Jiro Kondo

RSC ADVANCES   9 ( 40 )   22859 - 22862   2019年8月

A theoretical study on the elimination reaction of acrylonitrile from 2 '-O-cyanoethylated nucleosides by Bu4NF

Kaori Ando, Hisao Saneyoshi, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   75 ( 1 )   1 - 9   2019年1月

DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine

Takashi Kanamori, Yoshiaki Masaki, Yuki Oda, Hiroki Ohzeki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Organic and Biomolecular Chemistry   17 ( 8 )   2077 - 2080   2019年

Modification of oligonucleotides with weak basic residues: Via the 2′-O-carbamoylethyl linker for improving nuclease resistance without loss of duplex stability and antisense activity

Yoshiaki Masaki, Keishi Yamamoto, Keita Yoshida, Atsuya Maruyama, Takahito Tomori, Yusuke Iriyama, Hiroyuki Nakajima, Tatsuro Kanaki, Kohji Seio

Organic and Biomolecular Chemistry   17 ( 19 )   4835 - 4842   2019年

Effects of 2'-O-modifications on RNA duplex stability

Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   187 - 199   2018年12月

Nucleosides and oligonucleotides incorporating 2-thiothymine or 2-thiouracil derivatives as modified nucleobases

Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   115 - 130   2018年12月

Non-protected synthesis of oligonucleotides

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   3 - 16   2018年12月

Synthesis of and triplex formation in oligonucleotides containing 2’-deoxy-6-thioxanthosine 査読

Takeshi Inde, Shuhei Nishizawa, Yuusaku Hattori, Takashi Kanamori, Hideya Yuasa, Kohji Seio, Mitsuo Sekine, Akihiro Ohkubo

Bioorganic and Medicinal Chemistry   2018年7月

Solvent- and environment-dependent fluorescence of modified nucleobases

Kohji Seio, Takashi Kanamori, Yoshiaki Masaki

Tetrahedron Letters   59 ( 21 )   1977 - 1985   2018年5月

Synthesis of photocaged6-O-(2-nitrobenzyl) guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates for photocontrol of the RNA transcription reaction 査読

Kentaro Ohno, Daiki Sugiyama, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   25 ( 21 )   6007 - 6015   2017年11月

Synthesis of oligonucleotides containing 2-N-heteroarylguanine residues and their effect on duplex/triplex stability 査読

Takeshi Inde, Yoshiaki Masaki, Atsuya Maruyama, Yu Ito, Naoaki Makio, Yuya Miyatake, Takahito Tomori, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   15 ( 39 )   8371 - 8383   2017年10月

Deformability calculation for estimation of the relative stability of chemically modified RNA duplexes 査読

Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio

Current Protocols in Nucleic Acid Chemistry   2017   7.27.1 - 7.27.10   2017年3月

Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation 査読

Takashi Kanamori, Akihiro Takamura, Nobuhiro Tago, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   15 ( 5 )   1190 - 1197   2017年2月

Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl) propyl]thymidine 査読

Kohji Seio, Yurie Ohno, Kentaro Ohno, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   26 ( 19 )   4861 - 4863   2016年10月

Synthesis and Fluorescence Properties of Nucleosides with Pyrimidopyrimidine-Type Base Moieties 査読

Kohji Seio, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine

Fluorescent Analogs of Biomolecular Building Blocks: Design and Applications   208 - 223   2016年4月

Synthesis of 5-[3-(2-aminopyrimidin-4-yl)aminopropyn-1-yl]uracil derivative that recognizes Ade-Thy base pairs in double-stranded DNA 査読

Yu Ito, Yoshiaki Masaki, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   26 ( 1 )   194 - 196   2016年1月

Enzymatic synthesis and reverse transcription of RNAs incorporating 2 '-O-carbamoyl uridine triphosphate 査読

Yoshiaki Masaki, Hyugo Ito, Yuki Oda, Kazufumi Yamazaki, Nobuhiro Tago, Kentaro Ohno, Nozomi Ishii, Hirosuke Tsunoda, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

CHEMICAL COMMUNICATIONS   52 ( 87 )   12889 - 12892   2016年

Effective strategy for conformer-selective detection of short-lived excited state species: application to the IR spectroscopy of the N1H keto tautomer of guanine 査読

Hiroya Asami, Munefumi Tokugawa, Yoshiaki Masaki, Shun-ichi Ishiuchi, Eric Gloaguen, Kohji Seio, Hiroyuki Saigusa, Masaaki Fujii, Mitsuo Sekine, Michel Mons

Journal of Physical Chemistry A   2016年

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein 査読

Munefumi Tokugawa, Yoshiaki Masaki, Jan Christian Canggadibrata, Kazuhei Kaneko, Takashi Shiozawa, Takashi Kanamori, Morten Grotli, L. Marcus Wilhelmsson, Mitsuo Sekine, Kohji Seio

CHEMICAL COMMUNICATIONS   52 ( 19 )   3809 - 3812   2016年

A New Microfluidic Phase-Transfer Reaction Using HPLC Guard Columns as the Reactor for the N-3-Protection of Uridine Derivatives 査読

Nobuhiro Tago, Yoshiaki Masaki, Hiroshi Nagasawa, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

SYNLETT   26 ( 18 )   2578 - 2582   2015年11月

Design, Synthesis, and Properties of Phosphoramidate 2 ',5 '-Linked Branched RNA: Toward the Rational Design of Inhibitors of the RNA Lariat Debranching Enzyme 査読

Nobuhiro Tago, Adam Katolik, Nathaniel E. Clark, Eric J. Montemayor, Kohji Seio, Mitsuo Sekine, P. John Hart, Masad J. Damha

JOURNAL OF ORGANIC CHEMISTRY   80 ( 20 )   10108 - 10118   2015年10月

Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs 査読

Akihiro Ohkubo, Kenji Yamada, Yu Ito, Kiichi Yoshimura, Koichiro Miyauchi, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Hideya Yuasa, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   43 ( 12 )   5675 - 5686   2015年7月

Synthesis of Peptide Nucleic Acids Containing Pyridazine Derivatives As Cytosine and Thymine Analogs, and Their Duplexes with Complementary Oligodeoxynucleotides 査読

Takahito Tomori, Yuya Miyatake, Yuta Sato, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC LETTERS   17 ( 6 )   1609 - 1612   2015年3月

Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation 査読

Takashi Kanamori, Hiroki Ohzeki, Yoshiaki Masaki, Akihiro Ohkubo, Mari Takahashi, Kengo Tsuda, Takuhiro Ito, Mikako Shirouzu, Kanako Kuwasako, Yutaka Muto, Mitsuo Sekine, Kohji Seio

CHEMBIOCHEM   16 ( 1 )   167 - 176   2015年1月

Synthesis of Responsive Fluorescent Nucleobases 7-(Benzofuran-2-yl)-7-deazahypoxanthine and 7-(Benzofuran-2-yl)-7-deazaguanine Using Cross-coupling Reaction 査読

Munefumi Tokugawa, Kazuhei Kaneko, Masanori Saito, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

CHEMISTRY LETTERS   44 ( 1 )   64 - 66   2015年1月

Enhancement of exon skipping in mdx52 mice by 2′-O-methyl-2-thioribothymidine incorporation into phosphorothioate oligonucleotides

Yoshiaki Masaki, Takeshi Inde, Tetsuya Nagata, Jun Tanihata, Takashi Kanamori, Kohji Seio, Shin'ichi Takeda, Mitsuo Sekine

MedChemComm   6 ( 4 )   630 - 633   2015年

Synthesis and exon-skipping activity of chemically modified RNAs

Yoshiaki Masaki, Takeshi Yamada, Hisao Saneyoshi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications   497 - 510   2014年1月

Synthesis and properties of base or sugar modified RNA derivatives 招待

Mitsuo Sekine, Yoshiaki Masaki, Takeshi Yamada, Youdai Ishii, Keishi Yamamoto, Natsuki Okaniwa, Takeshi Kanamori, Hisashi Saneyoshi, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio

Collection of Czechoslovak Chemical Communications   14   167 - 170   2014年

Synthesis of branched DNA using oxidatively cleavable tritylsulfenyl as a hydroxy protecting group 査読

Kohji Seio, Takashi Kanamori, Mitsuo Sekine

Current Protocols in Nucleic Acid Chemistry   2014   2.18.1 - 2.18.19   2014年

Properties of 5- and/or 2-modified 2 '-O-cyanoethyl uridine residue: 2 '-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component 査読

Yoshiaki Masaki, Ryuta Miyasaka, Kunihiro Hirai, Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 7 )   1157 - 1162   2014年

A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds 査読

Ken Yamada, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 14 )   2255 - 2262   2014年

Synthesis and properties of oligonucleotides modified with 2 '-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives 査読

Takeshi Yamada, Yoshiaki Masaki, Natsuki Okaniwa, Takashi Kanamori, Akihiro Ohkubo, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 33 )   6457 - 6464   2014年

Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region 査読

Kohji Seio, Munefumi Tokugawa, Hirosuke Tsunoda, Akihiro Ohkubo, Fumio Arisaka, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   23 ( 24 )   6822 - 6824   2013年12月

Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3 '-terminus 査読

Yoshihiro Iijima, Shun Kojima, Erika Kodama, Sayako Kurohagi, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   11 ( 47 )   8276 - 8282   2013年12月

Chemical Synthesis of U1 snRNA Derivatives 査読

Akihiro Ohkubo, Yasushi Kondo, Makoto Suzuki, Haruki Kobayashi, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Kiyoshi Nagai, Mitsuo Sekine

ORGANIC LETTERS   15 ( 17 )   4386 - 4389   2013年9月

Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine. 国際誌

Kohji Seio, Takashi Kanamori, Munefumi Tokugawa, Hiroki Ohzeki, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

Bioorganic & medicinal chemistry   21 ( 11 )   3197 - 201   2013年6月

Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups

Kenji Yamada, Yusaku Hattori, Takeshi Inde, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Bioorganic and Medicinal Chemistry Letters   23 ( 3 )   776 - 778   2013年2月

DNA-maleimide: An improved maleimide compound for electrophoresis-based titration of reactive thiols in a specific protein 査読

Hara Satoshi, Nojima Tatsuya, Seio Kohji, Yoshida Masasuke, Hisabori Toru

Biochimica Et Biophysica Acta-General Subjects   1830 ( 4 )   3077 - 3081   2013年

Synthesis and properties of cationic 2 '-O-[N-(4-aminobutyl)carbamoyl] modified oligonucleotides 査読

Kohji Seio, Munefumi Tokugawa, Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   22 ( 7 )   2470 - 2473   2012年4月

Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts 査読

Taro Harakawa, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   22 ( 3 )   1445 - 1447   2012年2月

Synthesis of 5 '-Terminal Capped Oligonucleotides Using O-N Phosphoryl Migration of Phosphoramidite Derivatives 査読

Akihiro Ohkubo, Nobuhiro Tago, Akira Yokouchi, Yudai Nishino, Ken Yamada, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   14 ( 1 )   10 - 13   2012年1月

Formation of new base pairs between inosine and 5-methyl-2-thiocytidine derivatives 査読

Akihiro Ohkubo, Yudai Nishino, Yu Ito, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 10 )   2008 - 2010   2012年

DNA duplexes and triplex-forming oligodeoxynucleotides incorporating modified nucleosides forming stable and selective triplexes 査読

Takashi Kanamori, Yoshiaki Masaki, Masahiro Mizuta, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 5 )   1007 - 1013   2012年

Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2 '-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component 査読

Yoshiaki Masaki, Ryuta Miyasaka, Kunihiro Hirai, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

CHEMICAL COMMUNICATIONS   48 ( 58 )   7313 - 7315   2012年

Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents 査読

Kohji Seio, Sayako Kurohagi, Erika Kodama, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 5 )   994 - 1006   2012年

Synthesis of 2 '-O-[2-(N-Methylcarbamoyl)ethyl]ribonucleosides Using Oxa-Michael Reaction and Chemical and Biological Properties of Oligonucleotide Derivatives Incorporating These Modified Ribonucleosides 査読

Takeshi Yamada, Natsuki Okaniwa, Hisao Saneyoshi, Akihiro Ohkubo, Kohji Seio, Tetsuya Nagata, Yoshitsugu Aoki, Shin'ichi Takeda, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   76 ( 9 )   3042 - 3053   2011年5月

Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions 査読

Hirosuke Tsunoda, Tomomi Kudo, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   39 ( 7 )   2995 - 3004   2011年4月

Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine 査読

Shi-Qi Cao, Itaru Okamoto, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   52 ( 3 )   407 - 410   2011年1月

Stable triplex formation using the strong stacking effect of consecutive thionucleoside moieties 査読

Akihiro Ohkubo, Yudai Nishino, Akira Yokouchi, Yu Ito, Yasuhiro Noma, Yuuki Kakishima, Yoshiaki Masaki, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

CHEMICAL COMMUNICATIONS   47 ( 46 )   12556 - 12558   2011年

Synthesis and hybridization properties of 2 '-O-methylated oligoribonucleotides incorporating 2 '-O-naphthyluridines 査読

Mitsuo Sekine, Yusuke Oeda, Yoshihiro Iijima, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   9 ( 1 )   210 - 218   2011年

Synthesis and biological properties of 2'-O-modified oligoribonucleotide derivatives 招待

Takeshi Yamada, Natsuki Okaniwa, Yoshiaki Masaki, Yasuhiro Okuno, Ryuta Miyasaka, Hiroyuki Tsunoda, Akihiro Ohkubo, Kohji Seio, Tetsuya Nagata, Yoshitsugu Aoki, Shin'ichi Takeda, Mitsuo Sekine

Collection of Czechoslovak Chemical Communications   12   240 - 243   2011年

Synthesis of Oligodeoxynucleotides Using Fully Protected Deoxynucleoside 3 '-Phosphoramidite Building Blocks and Base Recognition of Oligodeoxynucleotides Incorporating N-3-Cyano-Ethylthymine 査読

Hirosuke Tsunoda, Tomomi Kudo, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

MOLECULES   15 ( 11 )   7509 - 7531   2010年11月

Synthesis and biochemical properties of oligodeoxynucleotides acylated by the chemically stable 2-(trimethylsilyl)benzoyl (TMSBz) group at the 5 ' or 3 ' terminus 査読

Ken Yamada, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   51 ( 39 )   5173 - 5176   2010年9月

Synthesis and Hybridization Properties of Oligonucleotides Incorporating Bi- and Tricyclic Cytosine Derivatives 査読

Akihiro Ohkubo, Toshiyuki Sakaue, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

CHEMISTRY LETTERS   39 ( 7 )   726 - 727   2010年7月

Oligonucleotide Synthesis Involving Deprotection of Amidine-Type Protecting Groups for Nucleobases under Acidic Conditions 査読

Akihiro Ohkubo, Yasukazu Kuwayama, Yudai Nishino, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   12 ( 11 )   2496 - 2499   2010年6月

Linear Relationship between Deformability and Thermal Stability of 2 '-O-Modified RNA Hetero Duplexes 査読

Yoshiaki Masaki, Ryuta Miyasaka, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

JOURNAL OF PHYSICAL CHEMISTRY B   114 ( 7 )   2517 - 2524   2010年2月

Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism 査読

Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   18 ( 2 )   567 - 572   2010年1月

Synthesis of oligodeoxynucleotides using the oxidatively cleavable 4-methoxytritylthio (MMTrS) group for protection of the 5 '-hydroxyl group 査読

Kohji Seio, Miyuki Shiraishi, Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine

NEW JOURNAL OF CHEMISTRY   34 ( 5 )   984 - 992   2010年

Synthesis and hybridization of 2 '-O-methyl-RNAs incorporating 2 '-O-carbamoyluridine and unique participation of the carbamoyl group in U-G base pair 査読

Kohji Seio, Ryuya Tawarada, Takeshi Sasami, Masashi Serizawa, Misako Ise, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 20 )   7275 - 7280   2009年10月

Synthesis and properties of nucleoside derivatives acylated by chemically stable 2-(trimethylsilyl)benzoyl group 査読

Ken Yamada, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 16 )   5928 - 5932   2009年8月

Fluorescence Properties of Pyrimidopyrimidoindole Nucleoside dC(PPI) Incorporated into Oligodeoxynucleotides 査読

Masahiro Mizuta, Kohji Seio, Akihiro Ohkubo, Mitsuo Sekine

JOURNAL OF PHYSICAL CHEMISTRY B   113 ( 28 )   9562 - 9569   2009年7月

Efficient solid-phase synthesis of oligodeoxynucleotides having a 5 '-terminal 2,2,7-trimethylguanosine pyrophosphate linkage 査読

Akihiro Ohkubo, Kenji Sasaki, Yasuhiro Noma, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 13 )   4819 - 4824   2009年7月

Introduction of 3 '-Terminal Nucleosides Having a Silyl-Type Linker into Polymer Supports without Base Protection 査読

Akihiro Ohkubo, Yasuhiro Noma, Katsufumi Aoki, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   74 ( 7 )   2817 - 2823   2009年4月

Computational evaluation of the stability of 2 '-O-methyl-RNA/RNA duplexes incorporating 3-deazaguanine derivatives by ab initio calculations and a molecular dynamics simulation 査読

Takeshi Sasami, Ryuya Tawarada, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM   899 ( 1-3 )   54 - 60   2009年4月

Synthesis of oligodeoxynucleotides incorporating 2-N-carbamoylguanine and evaluation of the hybridization properties 査読

Takeshi Sasami, Yoko Odawara, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 3 )   1398 - 1403   2009年2月

Synthesis of 4-Thiopseudoisocytidine and 4-Thiopseudouridine as Components of Triplex-forming Oligonucleotides 査読

Itaru Okamoto, Shiqi Cao, Hiroto Tanaka, Kohji Seio, Mitsuo Sekine

CHEMISTRY LETTERS   38 ( 2 )   174 - 175   2009年2月

Synthesis and Triplex Formation of Oligonucleotides Containing 8-Thioxodeoxyadenosine 査読

Ken-ichi Miyata, Ryuji Tamamushi, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   11 ( 3 )   605 - 608   2009年2月

Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine and 5-methyl-2-thiodeoxycytosine.

Akihiro Ohkubo, Kenichi Miyata, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   21 - 22   2009年

Evaluation of base recognition abilities of 2'-deoxynucleoside N-oxide derivatives by polymerase reactions.

Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   127 - 128   2009年

Development of new DNA triplex triads by using 5-substituted deoxycytidine.

Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 53 )   163 - 164   2009年

Mechanistic studies on oxidative condensation of a thymidine 3 '-H-phosphonate derivative with 3 '-O-acetylthymidine 査読

Ryuuya Tawarada, Kohji Seio, Mitsuo Sekine

ARKIVOC   264 - 273   2009年

Synthesis and hybridization properties of oligonucleotides having 4-N-(pyrrol-2-ylcarbonyl)deoxycytidine.

Ken Yamada, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   115 - 116   2009年

Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization.

Kohji Seio, Kazuya Miyazaki, Sayako Kurohagi, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   13 - 14   2009年

Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs 査読

Kohji Seio, Yusuke Takaku, Kazuya Miyazaki, Sayako Kurohagi, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   7 ( 11 )   2440 - 2451   2009年

New thermolytic carbamoyl groups for the protection of nucleobases 査読

Akihiro Ohkubo, Rintaro Kasuya, Kenichi Miyata, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   7 ( 4 )   687 - 694   2009年

Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues 査読

Kohji Seio, Masanhiro Mizuta, Kaori Tasaki, Keigo Tamaki, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 17 )   8287 - 8293   2008年9月

New nucleotide pairs for stable DNA triplexes stabilized by stacking interaction 査読

Masahiro Mizuta, Jun-ichi Banba, Takashi Kanamori, Ryuya Tawarada, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   130 ( 30 )   9622 - +   2008年7月

O-selective condensation using P-N bond cleavage in RNA synthesis without base protection 査読

Akihiro Ohkubo, Yasukazu Kuwayama, Tomomi Kudo, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   10 ( 13 )   2793 - 2796   2008年7月

Study of the base discrimination ability of DNA and 2 '-O-methylated RNA oligomers containing 2-thiouracil bases towards complementary RNA or DNA strands and their application to single base mismatch detection 査読

Itaru Okamoto, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 11 )   6034 - 6041   2008年6月

Efficient synthesis of functionalized oligodeoxyribonucleotides with base-labile groups using a new silyl linker 査読

Akihiro Ohkubo, Rintaro Kasuya, Katsufumi Aoki, Akio Kobori, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 9 )   5345 - 5351   2008年5月

Synthesis and hybridization properties of 2 '-O-(tetrazol-5-yl)ethyl-modified oligonucleotides 査読

Hisao Saneyoshi, Keigo Tamaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   64 ( 19 )   4370 - 4376   2008年5月

'Protected DNA Probes' capable of strong hybridization without removal of base protecting groups 査読

Akihiro Ohkubo, Rintaro Kasuya, Kazushi Sakamoto, Kenichi Miyata, Haruhiko Taguchi, Hiroshi Nagasawa, Toshifumi Tsukahara, Takuma Watanobe, Yoshiyuki Maki, Kohji Seio, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   36 ( 6 )   1952 - 1964   2008年4月

Synthesis and properties of DNA oligomers containing 2 '-deoxynucleoside N-oxide derivatives 査読

Hirosuke Tsunoda, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   73 ( 4 )   1217 - 1224   2008年2月

Synthesis and properties of oligonucleotides with iodo-substituted aromatic aglycons: Investigation of possible halogen bonding base pairs 査読

Ryuya Tawarada, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   73 ( 2 )   383 - 390   2008年1月

Synthesis and properties of oligodeoxynucleotides containing 5-carboxy-2'-deoxycytidines 査読

Masanori Sumino, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   18 ( 1 )   274 - 277   2008年1月

Protected DNA probes (PDP): A new strategy for gene detection 査読

Akihiro Ohkubo, Itaru Okamoto, Rintaro Kasuya, Kazushi Sakamoto, Takeshi Sasami, Yoko Odawara, Yoshiaki Masaki, Toshiyuki Sakaue, Hiroshi Nagasawa, Toshifumi Tsukahara, Kohji Seio, Mitsuo Sekine

CHEMISTRY OF NUCLEIC ACID COMPONENTS   10   94 - 102   2008年

An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis

Akihiro Ohkubo, Kunihiko Tanaka, Haruhiko Taguchi, Kohji Seio, Hiroshi Nagasawa, Toshifumi Tsukahara, Mitsuo Sekine

Bioorganic and Medicinal Chemistry Letters   17   5969 - 5973   2007年11月

Chemical synthesis of RNA via 2 '-O-cyanoethylated intermediates

Hisao Saneyoshi, Kaori Ando, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   63 ( 45 )   11195 - 11203   2007年11月

Facile synthesis of 2 '-O-cyanoethyluridine by ring-opening reaction of 2,2 '-anhydrouridine with cyanoethyl trimethylsilyl ether in the presence of BF3 center dot Et2O 査読

Hisao Saneyoshi, Itaru Okamoto, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   48 ( 48 )   8554 - 8557   2007年11月

Synthesis of branched Oligonucleotides with three different sequences using an oxidatively removable tritylthio group 査読

Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   72 ( 22 )   8259 - 8266   2007年10月

Fluorescent pyrimidopyrimidoindole nucleosides: Control of photophysical characterizations by substituent effects 査読

Masahiro Mizuta, Kohji Seio, Kenichi Miyata, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   72 ( 14 )   5046 - 5055   2007年7月

A new hydrophobic linker effective for the in situ synthesis of DNA-CPG conjugates as tools for SNP analysis 査読

Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Hiroshi Nagasawa, Toshifumi Tsukahara, Mitsuo Sekine

TETRAHEDRON LETTERS   48 ( 29 )   5147 - 5150   2007年7月

Synthesis and hybridization properties of oligodeoxynucleotides incorporating 2-N-carbamoylguanine derivatives as guanine analogs 査読

Takeshi Sasami, Yoko Odawara, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

TETRAHEDRON LETTERS   48 ( 30 )   5325 - 5329   2007年7月

Chemical properties of 4,5-di(ethoxycarbonyl)-1,3-dioxolan-2-yl (DECDO) as a hydroxyl protecting group of the 2 '-hydroxyl function in ribonucleosides 査読

Boleslaw Karwowski, Kohji Seio, Mitsuo Sekine

JOURNAL OF HETEROCYCLIC CHEMISTRY   44 ( 2 )   329 - 336   2007年3月

Highly Selective Recognition of Cytosine over Urasil and Adenine by a Guanine Analog, 2-N-Acetyl-3-deazaguanine, in 2’-O-Methyl-RNA/RNA Duplexes

Seio Kohji(Crest, JST, Sasami Takeshi(Crest, JST, Ohkubo Akihiro(Crest, JST, Ando Kaori(College of Education, University of the Ryukyus, Sekine Mitsuo(Dept. of Life, Science, Tokyo Institute of Technology

J. Am. Chem. Soc.   129 ( 5 )   1026 - 1027   2007年2月

A pyrimidopyrimidoindole nucleoside (dC(PPI)): Photophysical properties and thermal stability of the modified DNA duplexes 査読

Masahiro Mizuta, Kohji Seio, Kenichi Miyata, Akihiro Ohkubo, Haruhiko Taguchi, Mitsuo Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   26 ( 10-12 )   1335 - 1338   2007年

Development of an N-unprotected phosphoramidite method for the chemical synthesis of aminoacylated RNAs.

Akihiro Ohkubo, Yasuhiro Noma, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   1 - 2   2007年

Recognition of triplex forming oligodeoxynucleotides incorporating abasic sites by 5-arylcytosine residues in duplex DNAs.

Masahiro Mizuta, Jun-Ichi Banba, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   25 - 26   2007年

Development of a new method for the synthesis of oligodeoxynucleotides by use of carbamoyl-type protecting groups.

Akihiro Ohkubo, Rintaro Kasuya, Kenichi Miyata, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   131 - 132   2007年

Recognition of target chain-length by use of oligonucleotides having bulky substituents at the terminal bases.

Yusuke Takaku, Masahiro Mizuta, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   283 - 284   2007年

Synthesis and properties of oligodeoxynucleotides incorporating 5-carboxydeoxycytidine.

Masanori Sumino, Okamoto Itaru, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   165 - 166   2007年

Short-step chemical synthesis of DNA by use of MMTrS group for protection of 5'-hydroxyl group.

Miyuki Shiraishi, Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   129 - 130   2007年

Synthesis and hybridization properties of oligodeoxynucleotides with long-chain linkers 査読

Kohji Seio, Takeshi Terada, Masahiro Mizuta, Akihiro Ohkubo, Haruhiko Taguchi, Mitsuo Sekine

HELVETICA CHIMICA ACTA   90 ( 10 )   1946 - 1965   2007年

cis-tetrahydrofuran-3,4-diol structure as a key skeleton of new protecting groups removable by self-cyclization under oxidative conditions 査読

Eri Utagawa, Mitsuo Sekine, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   71 ( 20 )   7668 - 7677   2006年9月

Incorporation of 2 '-O-methyl-2-thiouridine into oligoribonucleotides induced stable A-form structure 査読

Okamoto, I, K Shohda, K Seio, M Sekine

CHEMISTRY LETTERS   35 ( 1 )   136 - 137   2006年1月

Synthesis and biological properties of new phosmidosine analogs having an N-acylsulfamate linkage 査読

H. Taguchi, A. Ohkubo, M. Sekine, K. Seio, H. Kakeya, H. Osada, T. Sasaki

Nucleosides, Nucleotides and Nucleic Acids   25 ( 4-6 )   647 - 654   2006年

A general method for the synthesis of 2 '-O-cyanoethylated oligoribonucleotides having promising hybridization affinity for DNA and RNA and enhanced nuclease resistance 査読

H Saneyoshi, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   70 ( 25 )   10453 - 10460   2005年12月

Chemically stabilized phenylboranylidene groups having a dimethoxytrityl group as a colorimetrically detectable protecting group designed for cis-1,2-diol functions of ribonucleosides in the solid-phase synthesis of m(2)(2,2)G5 ' ppT 査読

M Sekine, M Aoyagi, M Ushioda, A Ohkubo, K Seio

JOURNAL OF ORGANIC CHEMISTRY   70 ( 21 )   8400 - 8408   2005年10月

Computational evaluation of intermolecular interactions of a universal base 3-nitropyrrole in stacked dimers and DNA duplexes 査読

K Seio, H Ukawa, K Shohda, M Sekine

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS   22 ( 6 )   735 - 746   2005年6月

Mild and facile deprotection for the synthesis of oligodeoxynucleotide incorporating a 6-O-ethyl-deoxyguanosine 査読

K Seio, T Negishi, K Negishi, M Sekine

LETTERS IN ORGANIC CHEMISTRY   2 ( 2 )   179 - 183   2005年3月

Synthesis and hybridization properties of 2'-O-methyl-RNA incorporating 3-deazaguanine derivatives.

Kohji Seio, Takeshi Sasami, Akihiro Ohkubo, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 49 )   21 - 22   2005年

Synthesis and Properties of New Nucleotide Analogues Possessing Squaramide Moieties as New Phosphate Isosters

SEIO Kohji, SEIO Kohji, MIYASHITA Takuhei, MIYASHITA Takuhei, SATO Kousuke, SEKINE Mitsuo, SEKINE Mitsuo

Eur. J. Org. Chem.   2005 ( 24 )   5163 - 5170   2005年

[Development of new technologies directed toward superfine gene analysis].

Mitsuo Sekine, Akihiro Ohkubo, Itaru Okamoto, Akio Kobori, Kohji Seio

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   49 ( 17 Suppl )   2978 - 85   2004年12月

Synthesis of benzodithiol-2-yl-substituted nucleoside derivatives as lead compounds having anti-bovine viral diarrhea virus activity 査読

K Seio, T Sasaki, K Yanagida, M Baba, M Sekine

JOURNAL OF MEDICINAL CHEMISTRY   47 ( 21 )   5265 - 5275   2004年10月

Synthesis of enol adenosine 5 '-phosphate derivatives by the Perkow reaction of a silylated adenosine 5 '-phosphonate derivative with alpha-halo ketones 査読

T Moriguchi, K Okada, K Seio, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 3 )   246 - 248   2004年7月

Synthesis and Structural Properties of New Oligonucleotide Analogues Containing a 2’,5’-Internucleotidic Squaryldiamide Linkage Capable of Formation of Watson-Crick and Wobble Base Pairs at the 3’-Downstream Junction Site

SATO K, TAWARADA R, SEIO K, SEKINE M

Eur. J. Org. Chem.   ( 10 )   2142 - 2150   2004年

Synthesis of a biotin-conjugate of phosmidosine O-ethyl ester as a G1 arrest antitumor drug 査読

M. Sekine, K. Okada, K. Seio, T. Obata, T. Sasaki, H. Kakeya, H. Osada

Bioorganic and Medicinal Chemistry   12 ( 24 )   6343 - 6349   2004年

Structure-activity relationship of phosmidosine: Importance of the 7,8-dihydro-8-oxoadenosine residue for antitumor activity 査読

M. Sekine, K. Okada, K. Seio, H. Kakeya, H. Osada, T. Sasaki

Bioorganic and Medicinal Chemistry   12 ( 19 )   5193 - 5201   2004年

Synthesis of Chemically Stabilized Phosmidosine Analogues and the Structure-Activity Relationship of Phosmidosine 査読

M. Sekine, K. Okada, K. Seio, H. Kakeya, H. Osada, T. Obata, T. Sasaki

Journal of Organic Chemistry   69 ( 2 )   314 - 326   2004年

New protected protecting groups for the 5 '-hydroxy group of deoxynucleosides by use of 2-(hydroxymethyl)- and 2-[(methylamino)methyl]benzoyl skeletons and oxidatively cleavable tritylthio and (4-methoxytrityl)thio groups 査読

K Seio, E Utagawa, M Sekine

HELVETICA CHIMICA ACTA   87 ( 9 )   2318 - 2333   2004年

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab initio calculations.

Kohji Seio, Hisashi Ukawa, Koh-ichiro Shohda, Mitsuo Sekine

Nucleic acids research. Supplement (2001)   ( 3 )   49 - 50   2003年

Synthesis and biological properties of stable phosmidosine analogs. 査読

K. Okada, H. Taguchi, K. Seio, H. Kakeya, H. Osada, T. Sasaki, M. Sekine

Nucleic acids research. Supplement (2001)   ( 3 )   105 - 106   2003年

A convenient method for the conversion of beta-thymidine to alpha-thymidine based on TMSOTf-mediated C1 '-epimerization 査読

Y Sato, G Tateno, K Seio, M Sekine

TETRAHEDRON LETTERS   43 ( 17 )   3251 - 3254   2002年4月

Synthesis and Conformational Properties of Dinucleoside Triester Derivatives Involving a Cyclonucleotide Bridge Structure 査読

Sekine M, Kurasawa O, Shoda K, Seio K, Wada T

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   1989 - 1999   2001年

Synthesis and properties of a new oligonucleotide analogue containing an internucleotide squaryl amide linkage.

Sato K, Seio K, Sekine M

Nucleic acids symposium series   1   121 - 122   2001年

A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside bases.

A. Ohkubo, K. Seio, M. Sekine

Nucleic acids research. Supplement (2001)   ( 1 )   77 - 78   2001年

Fabrication of directly synthesized DNA chip using photolithography for rapid and parallel gene analysis 査読

K Takahashi, K Seio, M Sekine, O Hino, M Esashi

TRANSDUCERS '01: EUROSENSORS XV, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2   326 - 329   2001年

メチレン基のリン酸バックボーンへの挿入がRNase Hによるミスマッチを含む二重鎖切断に与える影響

田平彩乃, 服部詩萌里, 清尾康志, 正木慶昭

日本核酸医薬学会年会講演要旨集(CD-ROM)   8th   2023年

2’-O-カルバモイルエチルリンカーを有する修飾核酸X-CE-gapmer型アンチセンスの毒性低減効果とmRNAノックダウン作用-

入江雅彦, 細沢友紀, 西口由菜, 廣瀬優太, 加賀谷賢太, 日高夕貴, 窪山剛之, 正木慶昭, 清尾康志, 入山友輔

日本核酸医薬学会年会講演要旨集(CD-ROM)   8th   2023年

5 ́-デオキシ-5 ́-ヒドロキシメチルチミジン及び3 ́-デオキシ-3 ́-ヒドロキシメチルチミジンを含むリン酸骨格を伸長したオリゴヌクレオチドの合成と性質

正木慶昭, 正木慶昭, 田平彩乃, 服部詩萌里, 清尾康志

日本化学会春季年会講演予稿集(Web)   102nd   2022年

塩基部と5 ́位をスルホンアミド結合で架橋したチミジン誘導体の合成法の検討

金川峻幸, 立花茂載, 大西里絵, 正木慶昭, 正木慶昭, 清尾康志

日本化学会春季年会講演予稿集(Web)   102nd   2022年

スルホンアミド骨格を含む人工核酸の合成とアンチセンス活性の評価

大西里絵, 清尾康志, 正木慶昭, 正木慶昭, 関谷彰太, 枝村亘

日本化学会春季年会講演予稿集(Web)   102nd   2022年

Development of New Methods for Synthesis of Artificial Nucleic Acids having Various Functional Groups

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   72 ( 8 )   899 - 909   2014年8月

Base recognition of gap sites in DNA－DNA and DNA－RNA duplexes by short oligonucleotides

Ken Yamada, Yohsuke Esaka, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Bioorganic & Medicinal Chemistry Letters   23 ( 11 )   3448 - 3451   2013年

SYNTHESIS AND PROPERTIES OF OLIGONUCLEOTIDES HAVING 5 '-TERMINAL 2,2,7-TRIMETHYLGUANOSINE CAP ANALOGUES

Akihiro Ohkubo, Akira Yokouchi, Ken Yamada, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

NUCLEIC ACID THERAPEUTICS   21 ( 5 )   A39 - A40   2011年10月

2′-O-[(N-メチルカルバモイル)エチル]ウリジンを組み込んだRNAオリゴマーの合成と性質

山田剛史, 岡庭夏己, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本化学会講演予稿集   91st ( 3 )   2011年

2′水酸基修飾アンチセンスオリゴヌクレオチドの合成と筋ジストロフィーを対象としたエキソンスキッピング評価

山田剛史, 岡庭夏己, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本RNA学会年会要旨集   12th   2010年

5’末端にジリン酸結合を介してアデニル化されたオリゴヌクレオチドの効率合成法の開発

大窪章寛, 西野雄大, 野間靖弘, 桑山靖和, 角田浩佑, 清尾康志, 関根光雄

日本化学会講演予稿集   89th ( 2 )   2009年

プリンヌクレオシドへの2′-O-メチルカルバモイルエチル基の導入検討

山田剛史, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本RNA学会年会要旨集   11th   2009年

Binding of MutS protein to oligonucleotides containing a methylated- or an ethylated guanine residue, and correlation with mutation frequency

Kentro Taira, Shintaro Nakamura, Khota Nakano, Daisuke Maehara, Keinosuke Okamoto, S. Arimoto, D. Loakes, L. Worth, Jr, R. M. Schaaper, Kohji Seio, Mitsuo Sekine, Kazuo Negishi, Tomoe Negishi

Mutation Research   640   107 - 112   2008年

Synthesis and fluorescent properties of bi- and tricyclic 4-N-carbamoyldeoxycytidine derivatives

K.Miyata, R.Mineo, R.Tamamushi, M.Mizuta, A.Ohkubo, H.Taguchi, K.Seio, T.Santa

J Org Chem   72 ( 1 )   102 - 108   2007年

2’末端に極性をもった官能基を導入した修飾核酸の合成と性質

山田剛史, 山田剛史, 實吉尚郎, 大窪章寛, 大窪章寛, 清尾康志, 清尾康志, 関根光雄, 関根光雄

アンチセンスシンポジウム講演要旨集   17th   2007年

1,1-Dihydroperoxycyclododecaneas a new, crystalline non-hygroscopic oxidizer for the chemical synthesis of oligodeoxyribonucleotides

Hisao Saneyoshi, Kenichi Miyata, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   47 ( 50 )   8945 - 8947   2006年12月

Synthesis of 2 '-O-methyl-RNAs incorporating a 3-deazaguanine, and UV melting and computational studies on its hybridization properties

Kohji Seio, Takeshi Sasami, Ryuya Tawarada, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   34 ( 16 )   4324 - 4334   2006年9月

Conformational studies of 4-N-carbamoyldeoxycytidine derivatives and synthesis and hybridization properties of oligodeoxyribonucleotides incorporating these modified bases

Kenichi Miyata, Akio Kobori, Ryuji Tamamushi, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   2006 ( 16 )   3626 - 3637   2006年8月

Improved synthesis of oligonucleotides containing 2-thiouridine derivatives by use of diluted iodine solution

Okamoto, I, K Seio, M Sekine

TETRAHEDRON LETTERS   47 ( 4 )   583 - 585   2006年1月

DNA Synthesis without Base Protection Using the Phosphoramidite Approach

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Current Protocol in Nucleic Acid Chemistry   1 ( 3.15 )   1 - 20   2006年

Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative

K.Miyata, R.Tamamushi, A.Ohkubo, H.Taguchi, K.Seio, T.Santa, M.Sekine

Org Lett   8 ( 8 )   1545 - 1548   2006年

Synthesis of Deoxycytidine Derivative and Their Use for the Selective Photo Crosslinking with 5-Methylcytosine

Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   57 - 58   2006年

Synthesis and Properties of Artificial Base Pairs by Use of Halogen Bonds

Ryuta Tawarada, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   121 - 122   2006年

Synthesis and Properties of DNA Oligomers Containing 2'-Deoxycytidine-N-Oxide

Hirosuke Tsunoda Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   189 - 190   2006年

Use of ferrocene scaffolds as pendant groups in hairpin-type pyrrole-imidazole polyamide molecules showing sequence-selective binding to DNA duplexes

K Seio, M Mizuta, T Terada, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   70 ( 25 )   10311 - 10322   2005年12月

Synthesis of oligodeoxyribonucleotides containing hydroxymethylphosphonate bonds in the phosphoramidite method and their hybridization properties

A Ohkubo, K Aoki, Y Ezawa, Y Sato, H Taguchi, K Seio, M Sekine

TETRAHEDRON LETTERS   46 ( 51 )   8953 - 8957   2005年12月

Convenient synthesis of N-unprotected deoxynucleoside 3 '-phosphoramidite building blocks by selective deacylation of N-acylated species and their facile conversion to other N-functionalized derivatives

A Ohkubo, K Sakamoto, K Miyata, H Taguchi, K Seio, M Sekine

ORGANIC LETTERS   7 ( 24 )   5389 - 5392   2005年11月

Synthesis and conformational analysis of 4-N-carbamoyldeoxycytidine derivatives.

K Miyata, R Tamamushi, A Ohkubo, H Taguchi, K Seio, M Sekine

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   229   U560 - U560   2005年3月

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method.

A Ohkubo, H Taguchi, K Seio, M Sekine

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   229   U559 - U560   2005年3月

4,5-bis(ethoxycarbonyl)-[1,3]dioxolan-2-yl as a new orthoester-type protecting group for the 2 '-hydroxyl function in the chemical synthesis of RNA

B Karwowski, K Seio, M Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   24 ( 5-7 )   1111 - 1114   2005年

Analysis by Use of Protected Oligonucleotides Probes.

A. Ohkubo, R. Kasuya, K. Sakamoto, H. Taguchi, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   19 - 20   2005年

A new protecting group for the 5 '-hydroxyl group having O-S single bond oxidatively cleavable under mild conditions

E Utagawa, K Seio, M Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   24 ( 5-7 )   927 - 929   2005年

Development of New N-Unprotected Phosphoramidite Building Blocks Having a Silyl-Type Linker

A. Ohkubo, K. Aoki, H. Taguchi, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   127 - 128   2005年

Synthesis of 2'- or 3'-O-Heteroaryl Substituted Nucleic Acid Derivatives and Their Biological Properties

H. Taguchi, T. Narita, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   113 - 114   2005年

SNPs Analysis by Use of Oligonucleotides Containing 2'-O-Methyl-2-thiouridines.

I. Okamoto, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   123 - 124   2005年

Synthesis and hybridization affinity of oligodeoxyribonucleotides incorporating 4-N-(N-arylcarbamoyl)deoxycytidine derivatives

K Miyata, R Tamamushi, A Ohkubo, H Taguchi, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 51 )   9365 - 9368   2004年12月

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method

A Ohkubo, Y Ezawa, K Seio, M Sekine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   126 ( 35 )   10884 - 10896   2004年9月

Synthesis and properties of a pyrrole-imidazole polyamide having a ferrocene dicarboxylic amide linker

K Seio, M Mizuta, T Terada, M Sekine

TETRAHEDRON LETTERS   45 ( 36 )   6783 - 6786   2004年8月

Fine-tuning of acid susceptibility of 4,4 '-dimethoxytrityl ether derivatives by a methoxy group introduced via a styryl substituent

K Seio, T Sasaki, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 2 )   159 - 162   2004年4月

Synthesis and stability of 1-phenylethenyl phosphate derivatives and their phosphoryl transfer activity

T Moriguchi, K Okada, K Seio, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 2 )   140 - 144   2004年4月

A new approach for pyrophosphate bond formation starting from phosphoramidite derivatives by use of 6-trifluoromethyl-1-hydroxybenzotriazole-mediated O-N phosphoryl migration

A Ohkubo, K Aoki, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 5 )   979 - 982   2004年1月

A new strategy for the synthesis of oligodeoxynucleotides directed towards perfect O-selective internucleotidic bond formation without base protection

A Ohkubo, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 2 )   363 - 366   2004年1月

Synthesis and Base Recognition Ability of Oligoribonucleotides Incorporating 2-O-methyl-3-Deazaguanosine

Kohji Seio, Takeshi, Sasami Mitsuo Sekine

Nucleic Acids Symp. Series   48   65 - 66   2004年

Properties of Ferrocene-polyamide Compounds as Redox Active DNA binding Molecules toward SNPs Detection

Masahiro Mizuta, Takeshi Terada, Kohji Seio, Mitsuo Sekine

Nucleic Acids Symp. Series   48   237 - 238   2004年

A new route to 2 '-O-alkyl-2-thiouridine derivatives via 4-o-protection of the uracil base and hybridization properties of oligonucleotides incorporating these modified nucleoside derivatives

Okamoto, I, K Shohda, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   68 ( 26 )   9971 - 9982   2003年12月

Proton-block strategy for the synthesis of oligodeoxynucleotides without base protection, capping reaction, and P-N bond cleavage reaction

M Sekine, A Ohkubo, K Seio

JOURNAL OF ORGANIC CHEMISTRY   68 ( 14 )   5478 - 5492   2003年7月

Enhanced stereoselectivity in internucleotidic bond formation by the use of the chiral ribose moiety of thymidine

K Seio, K Kumura, JC Bologna, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   68 ( 10 )   3849 - 3859   2003年5月

Synthesis of TMG-capped RNA-DNA chimeric oligonucleotides

M Sekine, M Ushioda, T Wada, K Seio

TETRAHEDRON LETTERS   44 ( 8 )   1703 - 1707   2003年2月

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab intio calculations

Kohji Seio, Hisashi Ukawa, Koh-ichiro Shohda, Mitsuo Sekine

Nucleic Acids Res. Suppl.   3   49 - 50   2003年

Enhanced Stereoselectivity in Internucleotide Linkage Formation by the Effective Use of Intrinsically Chiral Ribose Miety of Nucleoside.

K. Seio, K. Kumura, M. Sekine

J. Org. Chem.   68   3849 - 3859   2003年

A Proton-Block Strategy in the Phosphoramidite Approach: A New N-Unprotected Method for the Synthesis of Oligodeoxynucleotides Based on the Principle of Simple Acid-Base Reactions.

M. Sekine, A. Ohkubo, K. Seio

J. Org. Chem.   68   5478 - 5492   2003年

Squaryl group as a new mimic of phosphate group in modified oligodeoxynucleotides: Synthesis and properties of new oligodeoxynucleotide analogues containing an internucleotidic squaryldiamide linkage

K Sato, K Seio, M Sekine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   124 ( 43 )   12715 - 12724   2002年10月

First synthesis and anticancer activity of phosmidosine and its related compounds

T Moriguchi, N Asai, K Okada, K Seio, T Sasaki, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   67 ( 10 )   3290 - 3300   2002年5月

A photochemical/chemical direct method of synthesizing high-performance deoxyribonucleic acid chips for rapid and parallel gene analysis

K Takahashii, K Seio, M Sekine, O Hino, M Esashi

SENSORS AND ACTUATORS B-CHEMICAL   83 ( 1-3 )   67 - 76   2002年3月

Substituent and solvent effects of TMS triflate mediated C1 ' epimerization of beta-thymidine to alpha-thymidine

Y Sato, G Tateno, K Seio, M Sekine

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   ( 1 )   87 - 93   2002年1月

A new method for the synthesis of oligodeoxyribonucleotides containing 4-N-alkoxycarbonyldeoxycytidine derivatives and their hybridization properties

A Kobori, K Miyata, M Ushioda, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   67 ( 2 )   476 - 485   2002年1月

A new silyl ether-type linker useful for the automated synthesis of oligonucleotides having base-labile protective groups

A Kobori, K Miyata, M Ushioda, K Seio, M Sekine

CHEMISTRY LETTERS   ( 1 )   16 - 17   2002年1月

Unique participation of unprotected internucleotidic phosphodiester residues on unexpected cleavage reaction of the Si-O bond of the diisopropylsilandiyl group used as a linker for the solid-phase synthesis of 5 '-terminal guanylated oligodeoxynucleotides

M Ushioda, M Kadokura, T Moriguchi, A Kobori, M Aoyagi, K Seio, M Sekine

HELVETICA CHIMICA ACTA   85 ( 9 )   2930 - 2945   2002年

A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   29 - 30   2002年

A new protecting group for 5'-hydroxyl function of nucleitides in oligonucleotide snthesis without acid treatment utilizing unique properties of tritylthio group

Kohji Seio Eri Utagawa, Mitsuo Sekine

Nucleic Acid Research Supplement   2   27 - 28   2002年

A new protecting group for 5'-hydroxyl function of nucleotides in oligonucleotide synthesis without acid treatment utilizing unique properties of tritylthio group

Kohji Seio Eri Utagawa, Mitsuo Sekine

Nucleic Acids Research Supplement   2   27 - 28   2002年

A New Finding in Connection with Side Reaction Associated with 5'-Terminal Pyrophosphorylation of Oligodeoxynucleotides on Polymer Supports: Selective Si-O Bond Cleavage by Strong Nucleophilicity of 4-Nitro-6-(triluoromethyl)-1H-1,2,3-benzotriazol-1-ol

M. Ushioda M, Kadokura, T. Moriguchi, A. Kobori, M. Aoyagi, K. Seio, M. Sekine

Helv. Chim. Acta.   85   2930 - 2945   2002年

Computational Analysis of Stacking Interactions betwee 3-Nitropyrrole and Natural Nucleobases

Hisashi Ukawa, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   173 - 174   2002年

Hybridization Ability and Base Pair Geometry of Modified Deoxycytidine Derivatives Having a 4-N-Carbamoyl Group

Kenichi Miyata Akio Kobori, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   161 - 162   2002年

Solid-phase synthesis of a 5 '-terminal TMG-capped trinucleotide block of U1 snRNA

M Kadokura, T Wada, K Seio, T Moriguchi, J Huber, R Luhrmann, M Sekine

TETRAHEDRON LETTERS   42 ( 50 )   8853 - 8856   2001年12月

Synthesis of pentathymidylate using a 4-monomethoxytritylthio (MMTrS) group as a 5 '-hydroxyl protecting group: toward oligonucleotide synthesis without acid treatment

K Seio, M Sekine

TETRAHEDRON LETTERS   42 ( 49 )   8657 - 8660   2001年12月

Total synthesis of agrocin 84 and phosmidosine as naturally occurring nucleotidic antibiotics having P-N bond linkages

M Sekine, T Moriguchi, T Wada, K Seio

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   59 ( 11 )   1109 - 1120   2001年11月

Essential factors for stabilization of the predominant C3 '-endo conformation in dinucleoside phosphotriester derivatives with cyclonucleotide bridge structures at the downstream 3 '-position

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   ( 10 )   1989 - 1999   2001年5月

Binding Specificities of the Mismatch Binding Protein, MutS, to Oligonucleotides Containing Modified bases

K. Negishi, D. Maehara, S. Nakamura, D. Loakes, l. Worth, Jr. R. M. Schaaper, K. Seio, M, Sekine T. Negishi

Nuceic Acids Research Supplement No1   221 - 222   2001年

Synthesis and Properties of a New Nucleotide Analogue Containing Squaryl Group

Kousuke Sato, Kohji Seio, Mitsuo Sekine

Nuceic Acids Research Supplement No1   121 - 122   2001年

A New Coupling Method for the Synthesis of Oligodeoxyribonucleotides Based on Protection by Protonation of Nnucleoside Bbases

A. Ohkubo, K. Seio, M. Sekine

Nuceic Acids Research Supplement No1   77 - 78   2001年

Substituent and Solvent Effects of TMS-triflate mediated C1'-Epimerization of beta-Thymidine to alpha-Thymidine

Yuichi Sato, Gohsuke, Tateno, Kohji Seio, Mitsuo Sekine

Eur. J. Org. Chem.   4583 - 4593   2001年

Synthesis and properties of oligonucleotides having a phosphorus chiral center by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

JOURNAL OF ORGANIC CHEMISTRY   65 ( 20 )   6515 - 6524   2000年10月

Synthesis and properties of 2 '-O-methyl-2-thiouridine and oligoribonucleotides containing 2 '-O-methyl-2-thiouridine

K Shohda, Okamoto, I, T Wada, K Seio, M Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   10 ( 16 )   1795 - 1798   2000年8月

Synthesis of 4-thiouridine, 6-thioinosine, and 6-thioguanosine 3 ',5 '-O-bisphosphates as donor molecules for RNA ligation and their application to the synthesis of photoactivatable TMG-capped U1 snRNA fragments

M Kadokura, T Wada, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   65 ( 17 )   5104 - 5113   2000年8月

Synthesis and antitumor activities of phosmidosine A and its N- acetylated derivative

Tomohisa Moriguchi, Norio Asai, Takeshi Wada, Kohji Seio, Takuma Sasaki, Mitsuo Sekine

Tetrahedron Letters   41 ( 31 )   5881 - 5885   2000年7月

Synthesis and properties of oligodeoxynucleotides incorporating a conformationally rigid uridine unit having a cyclic structure at the 5 '-terminal site

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

JOURNAL OF ORGANIC CHEMISTRY   65 ( 12 )   3571 - 3578   2000年6月

Synthesis and properties of oligothymidylates incorporating an artificial bend motif

K Seio, T Wada, M Sekine

HELVETICA CHIMICA ACTA   83 ( 1 )   162 - 180   2000年

Chemical synthesis of hydroxymethylphosophnate α-DNA

KOHJI SEIO

Nucleic Acids Sym. Ser.   44   31 - 32   2000年

Synthesis of N-labeled peptidyl AMP

H Narita, T Moriguchi, K Seio, M Sekine, H Miyaguchi, K Sakamoto, S Yokoyama

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   19 ( 10-12 )   1993 - 2003   2000年

Solid-phase synthesis of deoxyribonucleotides having a cap structure analog

KOHJI SEIO

Nucleic Acids Symp. Ser.   44   123 - 124   2000年

Synthesis of artificially bent oligonucleotides by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Seio, K Shohda, T Wada

NUCLEOSIDES & NUCLEOTIDES   18 ( 6-7 )   1163 - 1168   1999年

Synthesis of artificially bent oligonucleotides by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Seio, K Shohda, T Wada

NUCLEOSIDES & NUCLEOTIDES   18 ( 6-7 )   1163 - 1168   1999年

The Chemical Synthesis of Hypermethylated Capped RNA on Polymer Supports

M. Sekine, M, Kadokura T, Satoh T, Wada K. Seio

J. Mol. Science   15   318   1999年

Chemical synthesis and properties of conformationally fixed diuridine monophosphates as building blocks of the RNA turn motif

K Seio, T Wada, K Sakamoto, S Yokoyama, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   63 ( 5 )   1429 - 1443   1998年3月

Synthesis and properties of conformationally rigid cyclouridylic acids having covalent bonding linkers between the uracil 5-position and the 5'-phosphate group

K Seio, O Kurasawa, T Wada, K Sakamoto, S Yokoyama, M Sekine

NUCLEOSIDES & NUCLEOTIDES   16 ( 7-9 )   1023 - 1032   1997年

Chemical synthesis of a 5'-terminal TMG-capped triribonucleotide m(3)(2,2,7)G(5')pppAmpUmpA of U1 RNA

M Sekine, M Kadokura, T Satoh, K Seio, T Wada, U Fischer, Sumpter, V, R Luhrmann

JOURNAL OF ORGANIC CHEMISTRY   61 ( 13 )   4412 - 4422   1996年6月

Chemical synthesis and conformational properties of a new cyclouridylic acid having an ethylene bridge between the uracil 5-position and 5'-phosphate group

K Seio, T Wada, K Sakamoto, S Yokoyama, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   61 ( 4 )   1500 - 1504   1996年2月

Synthesis and properties of conformationally rigid cyclouridylic acid having a covalent bonding linker between the uracil 5-position and the 5′-phosphate group

Kohji Scio, Takeshi Wada, Kensaku Sakamoto, Shigeyuki Yokoyama, Mitsuo Sekine

Tetrahedron Letters   36 ( 52 )   9515 - 9518   1995年12月

Synthesis of oligonucleotide having a bent structure by incorporation of an interresidually cyclized uridylyl(3'-5')uridine unit

KOHJI SEIO

Nucleic Acids Symp. Ser.   34   181 - 182   1995年

CHEMICAL SYNTHESIS AND PROPERTIES OF AN INTERRESIDUALLY CYCLIZED URIDYLYL(3'-5')URIDINE AS A MODEL OF TRANSFER-RNA U-TURN STRUCTURE HAVING A SHARP BEND

M SEKINE, K SEIO, T WADA, K SAKAMOTO, S YOKOYAMA

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   116 ( 10 )   4469 - 4470   1994年5月

Chemical synthesis of the 5'-terminal structure of U1 RNA

KOHJI SEIO

Nucleic Acids Symp. Ser.   31   61 - 62   1994年

SYNTHESIS AND PROPERTIES OF N-TRITYLTHIO NUCLEOSIDE DERIVATIVES AND REDUCTIVE REMOVAL OF THE TRITYLTHIO GROUP BY USE OF TRIBUTYLTIN HYDRIDE AND TRIS(TRIMETHYLSILYL)SILANE

M SEKINE, K SEIO

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1   ( 24 )   3087 - 3093   1993年12月

SYNTHESIS OF URIDYLYL (3'-5') URIDINE DERIVATIVES CONTAINING 5-(METHYLAMINO-METHYL)URIDINE AS A MODIFIED NUCLEOSIDE FOUND FROM ESCHERICHIA-COLI MINOR TRANSFER RNA(ARG)

M SEKINE, K SEIO, T SATOH, K SAKAMOTO, S YOKOYAMA

NUCLEOSIDES & NUCLEOTIDES   12 ( 3-4 )   305 - 321   1993年

部位特異的に同位体修飾されたRNAのワンポット酵素合成

研究課題/領域番号：15K13738  2015年4月 - 2017年3月

日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

清尾 康志

三重鎖核酸を利用して制御可能な化学修飾遺伝子の開発

研究課題/領域番号：26288075  2014年4月 - 2017年3月

日本学術振興会  科学研究費助成事業  基盤研究(B)

清尾 康志

DNA-タンパク質相互作用の光制御を利用した遺伝子回路への光分子スイッチの導入

研究課題/領域番号：26119705  2014年4月 - 2016年3月

日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

清尾 康志

ローリングサークル増幅と蛍光三重鎖DNAを組合わせたmiRNA検出法

研究課題/領域番号：25620126  2013年4月 - 2015年3月

日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

清尾 康志

短鎖ＲＮＡ選択的結合能を有する人工核酸の特性を活用したｍｉＲＮＡ検出法の開発

研究課題/領域番号：23350077  2011年11月 - 2014年3月

日本学術振興会  科学研究費助成事業  基盤研究(B)

清尾 康志

三重鎖形成により制御可能な人工T7プロモータ

研究課題/領域番号：23655154  2011年

日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

清尾 康志

鎖長識別能を有する人工核酸プローブを利用したmiRNA検出法の開発

研究課題/領域番号：20350074  2008年 - 2010年

日本学術振興会  科学研究費助成事業  基盤研究(B)

清尾 康志

光結合性DNAとマイクロリアクタによる大規模高速アクエアスコンピューティング

研究課題/領域番号：17200022  2005年 - 2006年

日本学術振興会  科学研究費助成事業  基盤研究(A)

山村 雅幸, 清尾 康志, 藤井 輝夫, 藤本 健造

電気化学的モレキュラービーコンによる高速遺伝子検出法の開発

研究課題/領域番号：16651060  2004年

日本学術振興会  科学研究費助成事業  萌芽研究

清尾 康志

ゲノム修復型遺伝子治療を指向した、機能性DNAドラッグのデザインと創製

研究課題/領域番号：15012218  2003年

日本学術振興会  科学研究費助成事業  特定領域研究

清尾 康志

"プロトンブロック法"によるDNA・RNAの究極新合成法の開拓

研究課題/領域番号：14208074  2002年 - 2003年

日本学術振興会  科学研究費助成事業  基盤研究(A)

関根 光雄, 清尾 康志

ミスマッチ塩基対安定化能を有する人工DNAのデザインと開発

研究課題/領域番号：14011218  2002年

日本学術振興会  科学研究費助成事業  特定領域研究

清尾 康志

塩基配列を精密に認識するインテリジェント核酸プローブのデザインと開発

研究課題/領域番号：13202021  2001年

日本学術振興会  科学研究費助成事業  特定領域研究(C)

清尾 康志

核酸の新規化学合成法の開発とその応用

Developping new approaches toward the chamical synthesis of DNA and RNA.