Hic-5 antisense oligonucleotide inhibits advanced hepatic fibrosis and steatosis in vivo

Masahito Noguchi, Aya Miyauchi, Yoshiaki Masaki, Masashi Sakaki, Xiao-Feng Lei, Momoko Kobayashi-Tanabe, Akira Miyazaki, Takeshi Aoki, Hitoshi Yoshida, Kohji Seio, Joo-ri Kim-Kaneyama

JHEP REPORTS   6 ( 11 )   2024.11

Inhibitory Effects on RNA Binding and RNase H Induction Activity of Prodrug-Type Oligodeoxynucleotides Modified with a Galactosylated Self-Immolative Linker Cleavable by β-Galactosidase

Kento Miyaji, Yoshiaki Masaki, Kohji Seio

BIOCONJUGATE CHEMISTRY   35 ( 10 )   1587 - 1607   2024.10

Synthesis and Conformational Analyses of Cyclonucleoside Having 13-Membered Ring Bridging Nucleobase and 5′-Position via a Linker Containing Sulfonamide

Takayuki Kanagawa, Shigetoshi Tachibana, Yoshiaki Masaki, Kohji Seio

ORGANIC LETTERS   25 ( 43 )   7868 - 7872   2023.10

Alteration of target cleavage patterns and off-target reduction of antisense oligonucleotides incorporating 2-N-carbamoyl- or (2-pyridyl)guanine International journal

Takayuki Kanagawa, Aya Koyoma, Yoshiaki Masaki, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   21 ( 25 )   5214 - 5224   2023.6

Insertion of a methylene group into the backbone of an antisense oligonucleotide reveals the importance of deoxyribose recognition by RNase H

Yoshiaki Masaki, Ayano Tabira, Shihori Hattori, Shunsuke Wakatsuki, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   20 ( 45 )   8917 - 8924   2022.11

Synthesis of 2′-O-[3-(N-methylsulfamoyl)propan-1-yl]ribothymidine as a potentially applicable 2′-modified nucleoside for antisense oligonucleotides

Takahito Tomori, Koya Uekusa, Aya Koyama, Takayuki Kanagawa, Yoshiaki Masaki, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   73   2022.11

Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides

Yoshiaki Masaki, Yukiko Onishi, Kohji Seio

SCIENTIFIC REPORTS   12 ( 1 )   2022.7

Selective and stable base pairing by alkynylated nucleosides featuring a spatially-separated recognition interface

Hidenori Okamura, Giang Hoang Trinh, Zhuoxin Dong, Yoshiaki Masaki, Kohji Seio, Fumi Nagatsugi

NUCLEIC ACIDS RESEARCH   50 ( 6 )   3042 - 3055   2022.4

Oligodeoxynucleotides Modified with 2′-O-(Cysteinylaminobutyl)carbamoylethylribothymidine Residues for Native Chemical Ligation with Peptide at Internal Positions

Yoshiaki Masaki, Atsuya Maruyama, Keita Yoshida, Takahito Tomori, Tomohiro Kishimura, Kohji Seio

BIOCONJUGATE CHEMISTRY   33 ( 2 )   272 - 278   2022.2

Synthesis of 2′-O-alkylcarbamoylethyl-modified oligonucleotides with enhanced nuclease resistance that form isostable duplexes with complementary RNA

Tomohiro Kishimura, Takahito Tomori, Yoshiaki Masaki, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   35   2021.3

Novel EGFP reporter cell and mouse models for sensitive imaging and quantification of exon skipping

Yuko Hara, Yoshitaka Mizobe, Yukiko U. Inoue, Yasumasa Hashimoto, Norio Motohashi, Yoshiaki Masaki, Kohji Seio, Shin'ichi Takeda, Tetsuya Nagata, Matthew J. A. Wood, Takayoshi Inoue, Yoshitsugu Aoki

SCIENTIFIC REPORTS   10 ( 1 )   2020.6

Synthesis of Deoxypseudouridine 5′-Triphosphate Bearing the Photoremovable Protecting Group at the N1 Position Capable of Enzymatic Incorporation to DNA

Leo Takeshita, Yuji Yamada, Yoshiaki Masaki, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   85 ( 4 )   1861 - 1870   2020.2

Transcription of DNA duplex containing deoxypseudouridine and deoxypseudoisocytidine, and inhibition of transcription by triplex forming oligonucleotide that recognizes the modified duplex. International journal

Kohji Seio, Kei Yamaguchi, Ayano Yamazaki, Takashi Kanamori, Yoshiaki Masaki

Nucleosides, nucleotides & nucleic acids   39 ( 6 )   892 - 904   2020

Crystal structure of a DNA duplex cross-linked by 6-thioguanine-6-thioguanine disulfides: reversible formation and cleavage catalyzed by Cu(ii) ions and glutathione

Akira Ono, Takahiro Atsugi, Misato Goto, Hisao Saneyoshi, Takahito Tomori, Kohji Seio, Takenori Dairaku, Jiro Kondo

RSC ADVANCES   9 ( 40 )   22859 - 22862   2019.8

A theoretical study on the elimination reaction of acrylonitrile from 2 '-O-cyanoethylated nucleosides by Bu4NF

Kaori Ando, Hisao Saneyoshi, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   75 ( 1 )   1 - 9   2019.1

DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine

Takashi Kanamori, Yoshiaki Masaki, Yuki Oda, Hiroki Ohzeki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Organic and Biomolecular Chemistry   17 ( 8 )   2077 - 2080   2019

Modification of oligonucleotides with weak basic residues: Via the 2′-O-carbamoylethyl linker for improving nuclease resistance without loss of duplex stability and antisense activity

Yoshiaki Masaki, Keishi Yamamoto, Keita Yoshida, Atsuya Maruyama, Takahito Tomori, Yusuke Iriyama, Hiroyuki Nakajima, Tatsuro Kanaki, Kohji Seio

Organic and Biomolecular Chemistry   17 ( 19 )   4835 - 4842   2019

Effects of 2'-O-modifications on RNA duplex stability

Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   187 - 199   2018.12

Nucleosides and oligonucleotides incorporating 2-thiothymine or 2-thiouracil derivatives as modified nucleobases

Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   115 - 130   2018.12

Non-protected synthesis of oligonucleotides

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Synthesis of Therapeutic Oligonucleotides   3 - 16   2018.12

Synthesis of and triplex formation in oligonucleotides containing 2’-deoxy-6-thioxanthosine Reviewed

Takeshi Inde, Shuhei Nishizawa, Yuusaku Hattori, Takashi Kanamori, Hideya Yuasa, Kohji Seio, Mitsuo Sekine, Akihiro Ohkubo

Bioorganic and Medicinal Chemistry   2018.7

Solvent- and environment-dependent fluorescence of modified nucleobases

Kohji Seio, Takashi Kanamori, Yoshiaki Masaki

Tetrahedron Letters   59 ( 21 )   1977 - 1985   2018.5

Synthesis of photocaged6-O-(2-nitrobenzyl) guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates for photocontrol of the RNA transcription reaction Reviewed

Kentaro Ohno, Daiki Sugiyama, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   25 ( 21 )   6007 - 6015   2017.11

Synthesis of oligonucleotides containing 2-N-heteroarylguanine residues and their effect on duplex/triplex stability Reviewed

Takeshi Inde, Yoshiaki Masaki, Atsuya Maruyama, Yu Ito, Naoaki Makio, Yuya Miyatake, Takahito Tomori, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   15 ( 39 )   8371 - 8383   2017.10

Deformability calculation for estimation of the relative stability of chemically modified RNA duplexes Reviewed

Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio

Current Protocols in Nucleic Acid Chemistry   2017   7.27.1 - 7.27.10   2017.3

Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation Reviewed

Takashi Kanamori, Akihiro Takamura, Nobuhiro Tago, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   15 ( 5 )   1190 - 1197   2017.2

Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl) propyl]thymidine Reviewed

Kohji Seio, Yurie Ohno, Kentaro Ohno, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   26 ( 19 )   4861 - 4863   2016.10

Synthesis and Fluorescence Properties of Nucleosides with Pyrimidopyrimidine-Type Base Moieties Reviewed

Kohji Seio, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine

Fluorescent Analogs of Biomolecular Building Blocks: Design and Applications   208 - 223   2016.4

Synthesis of 5-[3-(2-aminopyrimidin-4-yl)aminopropyn-1-yl]uracil derivative that recognizes Ade-Thy base pairs in double-stranded DNA Reviewed

Yu Ito, Yoshiaki Masaki, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   26 ( 1 )   194 - 196   2016.1

Enzymatic synthesis and reverse transcription of RNAs incorporating 2 '-O-carbamoyl uridine triphosphate Reviewed

Yoshiaki Masaki, Hyugo Ito, Yuki Oda, Kazufumi Yamazaki, Nobuhiro Tago, Kentaro Ohno, Nozomi Ishii, Hirosuke Tsunoda, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

CHEMICAL COMMUNICATIONS   52 ( 87 )   12889 - 12892   2016

Effective strategy for conformer-selective detection of short-lived excited state species: application to the IR spectroscopy of the N1H keto tautomer of guanine Reviewed

Hiroya Asami, Munefumi Tokugawa, Yoshiaki Masaki, Shun-ichi Ishiuchi, Eric Gloaguen, Kohji Seio, Hiroyuki Saigusa, Masaaki Fujii, Mitsuo Sekine, Michel Mons

Journal of Physical Chemistry A   2016

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein Reviewed

Munefumi Tokugawa, Yoshiaki Masaki, Jan Christian Canggadibrata, Kazuhei Kaneko, Takashi Shiozawa, Takashi Kanamori, Morten Grotli, L. Marcus Wilhelmsson, Mitsuo Sekine, Kohji Seio

CHEMICAL COMMUNICATIONS   52 ( 19 )   3809 - 3812   2016

A New Microfluidic Phase-Transfer Reaction Using HPLC Guard Columns as the Reactor for the N-3-Protection of Uridine Derivatives Reviewed

Nobuhiro Tago, Yoshiaki Masaki, Hiroshi Nagasawa, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

SYNLETT   26 ( 18 )   2578 - 2582   2015.11

Design, Synthesis, and Properties of Phosphoramidate 2 ',5 '-Linked Branched RNA: Toward the Rational Design of Inhibitors of the RNA Lariat Debranching Enzyme Reviewed

Nobuhiro Tago, Adam Katolik, Nathaniel E. Clark, Eric J. Montemayor, Kohji Seio, Mitsuo Sekine, P. John Hart, Masad J. Damha

JOURNAL OF ORGANIC CHEMISTRY   80 ( 20 )   10108 - 10118   2015.10

Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs Reviewed

Akihiro Ohkubo, Kenji Yamada, Yu Ito, Kiichi Yoshimura, Koichiro Miyauchi, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Hideya Yuasa, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   43 ( 12 )   5675 - 5686   2015.7

Synthesis of Peptide Nucleic Acids Containing Pyridazine Derivatives As Cytosine and Thymine Analogs, and Their Duplexes with Complementary Oligodeoxynucleotides Reviewed

Takahito Tomori, Yuya Miyatake, Yuta Sato, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC LETTERS   17 ( 6 )   1609 - 1612   2015.3

Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation Reviewed

Takashi Kanamori, Hiroki Ohzeki, Yoshiaki Masaki, Akihiro Ohkubo, Mari Takahashi, Kengo Tsuda, Takuhiro Ito, Mikako Shirouzu, Kanako Kuwasako, Yutaka Muto, Mitsuo Sekine, Kohji Seio

CHEMBIOCHEM   16 ( 1 )   167 - 176   2015.1

Synthesis of Responsive Fluorescent Nucleobases 7-(Benzofuran-2-yl)-7-deazahypoxanthine and 7-(Benzofuran-2-yl)-7-deazaguanine Using Cross-coupling Reaction Reviewed

Munefumi Tokugawa, Kazuhei Kaneko, Masanori Saito, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

CHEMISTRY LETTERS   44 ( 1 )   64 - 66   2015.1

Enhancement of exon skipping in mdx52 mice by 2′-O-methyl-2-thioribothymidine incorporation into phosphorothioate oligonucleotides

Yoshiaki Masaki, Takeshi Inde, Tetsuya Nagata, Jun Tanihata, Takashi Kanamori, Kohji Seio, Shin'ichi Takeda, Mitsuo Sekine

MedChemComm   6 ( 4 )   630 - 633   2015

Synthesis and exon-skipping activity of chemically modified RNAs

Yoshiaki Masaki, Takeshi Yamada, Hisao Saneyoshi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications   497 - 510   2014.1

Synthesis and properties of base or sugar modified RNA derivatives Invited

Mitsuo Sekine, Yoshiaki Masaki, Takeshi Yamada, Youdai Ishii, Keishi Yamamoto, Natsuki Okaniwa, Takeshi Kanamori, Hisashi Saneyoshi, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio

Collection of Czechoslovak Chemical Communications   14   167 - 170   2014

Synthesis of branched DNA using oxidatively cleavable tritylsulfenyl as a hydroxy protecting group Reviewed

Kohji Seio, Takashi Kanamori, Mitsuo Sekine

Current Protocols in Nucleic Acid Chemistry   2014   2.18.1 - 2.18.19   2014

Properties of 5- and/or 2-modified 2 '-O-cyanoethyl uridine residue: 2 '-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component Reviewed

Yoshiaki Masaki, Ryuta Miyasaka, Kunihiro Hirai, Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 7 )   1157 - 1162   2014

A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds Reviewed

Ken Yamada, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 14 )   2255 - 2262   2014

Synthesis and properties of oligonucleotides modified with 2 '-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives Reviewed

Takeshi Yamada, Yoshiaki Masaki, Natsuki Okaniwa, Takashi Kanamori, Akihiro Ohkubo, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   12 ( 33 )   6457 - 6464   2014

Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region Reviewed

Kohji Seio, Munefumi Tokugawa, Hirosuke Tsunoda, Akihiro Ohkubo, Fumio Arisaka, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   23 ( 24 )   6822 - 6824   2013.12

Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3 '-terminus Reviewed

Yoshihiro Iijima, Shun Kojima, Erika Kodama, Sayako Kurohagi, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   11 ( 47 )   8276 - 8282   2013.12

Chemical Synthesis of U1 snRNA Derivatives Reviewed

Akihiro Ohkubo, Yasushi Kondo, Makoto Suzuki, Haruki Kobayashi, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Kiyoshi Nagai, Mitsuo Sekine

ORGANIC LETTERS   15 ( 17 )   4386 - 4389   2013.9

Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine. International journal

Kohji Seio, Takashi Kanamori, Munefumi Tokugawa, Hiroki Ohzeki, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

Bioorganic & medicinal chemistry   21 ( 11 )   3197 - 201   2013.6

Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups

Kenji Yamada, Yusaku Hattori, Takeshi Inde, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Bioorganic and Medicinal Chemistry Letters   23 ( 3 )   776 - 778   2013.2

DNA-maleimide: An improved maleimide compound for electrophoresis-based titration of reactive thiols in a specific protein Reviewed

Hara Satoshi, Nojima Tatsuya, Seio Kohji, Yoshida Masasuke, Hisabori Toru

Biochimica Et Biophysica Acta-General Subjects   1830 ( 4 )   3077 - 3081   2013

Synthesis and properties of cationic 2 '-O-[N-(4-aminobutyl)carbamoyl] modified oligonucleotides Reviewed

Kohji Seio, Munefumi Tokugawa, Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   22 ( 7 )   2470 - 2473   2012.4

Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts Reviewed

Taro Harakawa, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   22 ( 3 )   1445 - 1447   2012.2

Synthesis of 5 '-Terminal Capped Oligonucleotides Using O-N Phosphoryl Migration of Phosphoramidite Derivatives Reviewed

Akihiro Ohkubo, Nobuhiro Tago, Akira Yokouchi, Yudai Nishino, Ken Yamada, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   14 ( 1 )   10 - 13   2012.1

Formation of new base pairs between inosine and 5-methyl-2-thiocytidine derivatives Reviewed

Akihiro Ohkubo, Yudai Nishino, Yu Ito, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 10 )   2008 - 2010   2012

DNA duplexes and triplex-forming oligodeoxynucleotides incorporating modified nucleosides forming stable and selective triplexes Reviewed

Takashi Kanamori, Yoshiaki Masaki, Masahiro Mizuta, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 5 )   1007 - 1013   2012

Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2 '-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component Reviewed

Yoshiaki Masaki, Ryuta Miyasaka, Kunihiro Hirai, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

CHEMICAL COMMUNICATIONS   48 ( 58 )   7313 - 7315   2012

Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents Reviewed

Kohji Seio, Sayako Kurohagi, Erika Kodama, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   10 ( 5 )   994 - 1006   2012

Synthesis of 2 '-O-[2-(N-Methylcarbamoyl)ethyl]ribonucleosides Using Oxa-Michael Reaction and Chemical and Biological Properties of Oligonucleotide Derivatives Incorporating These Modified Ribonucleosides Reviewed

Takeshi Yamada, Natsuki Okaniwa, Hisao Saneyoshi, Akihiro Ohkubo, Kohji Seio, Tetsuya Nagata, Yoshitsugu Aoki, Shin'ichi Takeda, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   76 ( 9 )   3042 - 3053   2011.5

Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions Reviewed

Hirosuke Tsunoda, Tomomi Kudo, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   39 ( 7 )   2995 - 3004   2011.4

Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine Reviewed

Shi-Qi Cao, Itaru Okamoto, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   52 ( 3 )   407 - 410   2011.1

Stable triplex formation using the strong stacking effect of consecutive thionucleoside moieties Reviewed

Akihiro Ohkubo, Yudai Nishino, Akira Yokouchi, Yu Ito, Yasuhiro Noma, Yuuki Kakishima, Yoshiaki Masaki, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

CHEMICAL COMMUNICATIONS   47 ( 46 )   12556 - 12558   2011

Synthesis and hybridization properties of 2 '-O-methylated oligoribonucleotides incorporating 2 '-O-naphthyluridines Reviewed

Mitsuo Sekine, Yusuke Oeda, Yoshihiro Iijima, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio

ORGANIC & BIOMOLECULAR CHEMISTRY   9 ( 1 )   210 - 218   2011

Synthesis and biological properties of 2'-O-modified oligoribonucleotide derivatives Invited

Takeshi Yamada, Natsuki Okaniwa, Yoshiaki Masaki, Yasuhiro Okuno, Ryuta Miyasaka, Hiroyuki Tsunoda, Akihiro Ohkubo, Kohji Seio, Tetsuya Nagata, Yoshitsugu Aoki, Shin'ichi Takeda, Mitsuo Sekine

Collection of Czechoslovak Chemical Communications   12   240 - 243   2011

Synthesis of Oligodeoxynucleotides Using Fully Protected Deoxynucleoside 3 '-Phosphoramidite Building Blocks and Base Recognition of Oligodeoxynucleotides Incorporating N-3-Cyano-Ethylthymine Reviewed

Hirosuke Tsunoda, Tomomi Kudo, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

MOLECULES   15 ( 11 )   7509 - 7531   2010.11

Synthesis and biochemical properties of oligodeoxynucleotides acylated by the chemically stable 2-(trimethylsilyl)benzoyl (TMSBz) group at the 5 ' or 3 ' terminus Reviewed

Ken Yamada, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   51 ( 39 )   5173 - 5176   2010.9

Synthesis and Hybridization Properties of Oligonucleotides Incorporating Bi- and Tricyclic Cytosine Derivatives Reviewed

Akihiro Ohkubo, Toshiyuki Sakaue, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

CHEMISTRY LETTERS   39 ( 7 )   726 - 727   2010.7

Oligonucleotide Synthesis Involving Deprotection of Amidine-Type Protecting Groups for Nucleobases under Acidic Conditions Reviewed

Akihiro Ohkubo, Yasukazu Kuwayama, Yudai Nishino, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   12 ( 11 )   2496 - 2499   2010.6

Linear Relationship between Deformability and Thermal Stability of 2 '-O-Modified RNA Hetero Duplexes Reviewed

Yoshiaki Masaki, Ryuta Miyasaka, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

JOURNAL OF PHYSICAL CHEMISTRY B   114 ( 7 )   2517 - 2524   2010.2

Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism Reviewed

Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   18 ( 2 )   567 - 572   2010.1

Synthesis of oligodeoxynucleotides using the oxidatively cleavable 4-methoxytritylthio (MMTrS) group for protection of the 5 '-hydroxyl group Reviewed

Kohji Seio, Miyuki Shiraishi, Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine

NEW JOURNAL OF CHEMISTRY   34 ( 5 )   984 - 992   2010

Synthesis and hybridization of 2 '-O-methyl-RNAs incorporating 2 '-O-carbamoyluridine and unique participation of the carbamoyl group in U-G base pair Reviewed

Kohji Seio, Ryuya Tawarada, Takeshi Sasami, Masashi Serizawa, Misako Ise, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 20 )   7275 - 7280   2009.10

Synthesis and properties of nucleoside derivatives acylated by chemically stable 2-(trimethylsilyl)benzoyl group Reviewed

Ken Yamada, Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 16 )   5928 - 5932   2009.8

Fluorescence Properties of Pyrimidopyrimidoindole Nucleoside dC(PPI) Incorporated into Oligodeoxynucleotides Reviewed

Masahiro Mizuta, Kohji Seio, Akihiro Ohkubo, Mitsuo Sekine

JOURNAL OF PHYSICAL CHEMISTRY B   113 ( 28 )   9562 - 9569   2009.7

Efficient solid-phase synthesis of oligodeoxynucleotides having a 5 '-terminal 2,2,7-trimethylguanosine pyrophosphate linkage Reviewed

Akihiro Ohkubo, Kenji Sasaki, Yasuhiro Noma, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 13 )   4819 - 4824   2009.7

Introduction of 3 '-Terminal Nucleosides Having a Silyl-Type Linker into Polymer Supports without Base Protection Reviewed

Akihiro Ohkubo, Yasuhiro Noma, Katsufumi Aoki, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   74 ( 7 )   2817 - 2823   2009.4

Computational evaluation of the stability of 2 '-O-methyl-RNA/RNA duplexes incorporating 3-deazaguanine derivatives by ab initio calculations and a molecular dynamics simulation Reviewed

Takeshi Sasami, Ryuya Tawarada, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM   899 ( 1-3 )   54 - 60   2009.4

Synthesis of oligodeoxynucleotides incorporating 2-N-carbamoylguanine and evaluation of the hybridization properties Reviewed

Takeshi Sasami, Yoko Odawara, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

BIOORGANIC & MEDICINAL CHEMISTRY   17 ( 3 )   1398 - 1403   2009.2

Synthesis of 4-Thiopseudoisocytidine and 4-Thiopseudouridine as Components of Triplex-forming Oligonucleotides Reviewed

Itaru Okamoto, Shiqi Cao, Hiroto Tanaka, Kohji Seio, Mitsuo Sekine

CHEMISTRY LETTERS   38 ( 2 )   174 - 175   2009.2

Synthesis and Triplex Formation of Oligonucleotides Containing 8-Thioxodeoxyadenosine Reviewed

Ken-ichi Miyata, Ryuji Tamamushi, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   11 ( 3 )   605 - 608   2009.2

Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine and 5-methyl-2-thiodeoxycytosine.

Akihiro Ohkubo, Kenichi Miyata, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   21 - 22   2009

Evaluation of base recognition abilities of 2'-deoxynucleoside N-oxide derivatives by polymerase reactions.

Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   127 - 128   2009

Development of new DNA triplex triads by using 5-substituted deoxycytidine.

Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 53 )   163 - 164   2009

Mechanistic studies on oxidative condensation of a thymidine 3 '-H-phosphonate derivative with 3 '-O-acetylthymidine Reviewed

Ryuuya Tawarada, Kohji Seio, Mitsuo Sekine

ARKIVOC   264 - 273   2009

Synthesis and hybridization properties of oligonucleotides having 4-N-(pyrrol-2-ylcarbonyl)deoxycytidine.

Ken Yamada, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   115 - 116   2009

Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization.

Kohji Seio, Kazuya Miyazaki, Sayako Kurohagi, Yoshiaki Masaki, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 53 )   13 - 14   2009

Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs Reviewed

Kohji Seio, Yusuke Takaku, Kazuya Miyazaki, Sayako Kurohagi, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   7 ( 11 )   2440 - 2451   2009

New thermolytic carbamoyl groups for the protection of nucleobases Reviewed

Akihiro Ohkubo, Rintaro Kasuya, Kenichi Miyata, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC & BIOMOLECULAR CHEMISTRY   7 ( 4 )   687 - 694   2009

Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues Reviewed

Kohji Seio, Masanhiro Mizuta, Kaori Tasaki, Keigo Tamaki, Akihiro Ohkubo, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 17 )   8287 - 8293   2008.9

New nucleotide pairs for stable DNA triplexes stabilized by stacking interaction Reviewed

Masahiro Mizuta, Jun-ichi Banba, Takashi Kanamori, Ryuya Tawarada, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   130 ( 30 )   9622 - +   2008.7

O-selective condensation using P-N bond cleavage in RNA synthesis without base protection Reviewed

Akihiro Ohkubo, Yasukazu Kuwayama, Tomomi Kudo, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

ORGANIC LETTERS   10 ( 13 )   2793 - 2796   2008.7

Study of the base discrimination ability of DNA and 2 '-O-methylated RNA oligomers containing 2-thiouracil bases towards complementary RNA or DNA strands and their application to single base mismatch detection Reviewed

Itaru Okamoto, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 11 )   6034 - 6041   2008.6

Efficient synthesis of functionalized oligodeoxyribonucleotides with base-labile groups using a new silyl linker Reviewed

Akihiro Ohkubo, Rintaro Kasuya, Katsufumi Aoki, Akio Kobori, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY   16 ( 9 )   5345 - 5351   2008.5

Synthesis and hybridization properties of 2 '-O-(tetrazol-5-yl)ethyl-modified oligonucleotides Reviewed

Hisao Saneyoshi, Keigo Tamaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   64 ( 19 )   4370 - 4376   2008.5

'Protected DNA Probes' capable of strong hybridization without removal of base protecting groups Reviewed

Akihiro Ohkubo, Rintaro Kasuya, Kazushi Sakamoto, Kenichi Miyata, Haruhiko Taguchi, Hiroshi Nagasawa, Toshifumi Tsukahara, Takuma Watanobe, Yoshiyuki Maki, Kohji Seio, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   36 ( 6 )   1952 - 1964   2008.4

Synthesis and properties of DNA oligomers containing 2 '-deoxynucleoside N-oxide derivatives Reviewed

Hirosuke Tsunoda, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   73 ( 4 )   1217 - 1224   2008.2

Synthesis and properties of oligonucleotides with iodo-substituted aromatic aglycons: Investigation of possible halogen bonding base pairs Reviewed

Ryuya Tawarada, Kohji Seio, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   73 ( 2 )   383 - 390   2008.1

Synthesis and properties of oligodeoxynucleotides containing 5-carboxy-2'-deoxycytidines Reviewed

Masanori Sumino, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   18 ( 1 )   274 - 277   2008.1

Protected DNA probes (PDP): A new strategy for gene detection Reviewed

Akihiro Ohkubo, Itaru Okamoto, Rintaro Kasuya, Kazushi Sakamoto, Takeshi Sasami, Yoko Odawara, Yoshiaki Masaki, Toshiyuki Sakaue, Hiroshi Nagasawa, Toshifumi Tsukahara, Kohji Seio, Mitsuo Sekine

CHEMISTRY OF NUCLEIC ACID COMPONENTS   10   94 - 102   2008

An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis

Akihiro Ohkubo, Kunihiko Tanaka, Haruhiko Taguchi, Kohji Seio, Hiroshi Nagasawa, Toshifumi Tsukahara, Mitsuo Sekine

Bioorganic and Medicinal Chemistry Letters   17   5969 - 5973   2007.11

Chemical synthesis of RNA via 2 '-O-cyanoethylated intermediates

Hisao Saneyoshi, Kaori Ando, Kohji Seio, Mitsuo Sekine

TETRAHEDRON   63 ( 45 )   11195 - 11203   2007.11

Facile synthesis of 2 '-O-cyanoethyluridine by ring-opening reaction of 2,2 '-anhydrouridine with cyanoethyl trimethylsilyl ether in the presence of BF3 center dot Et2O Reviewed

Hisao Saneyoshi, Itaru Okamoto, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   48 ( 48 )   8554 - 8557   2007.11

Synthesis of branched Oligonucleotides with three different sequences using an oxidatively removable tritylthio group Reviewed

Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   72 ( 22 )   8259 - 8266   2007.10

Fluorescent pyrimidopyrimidoindole nucleosides: Control of photophysical characterizations by substituent effects Reviewed

Masahiro Mizuta, Kohji Seio, Kenichi Miyata, Mitsuo Sekine

JOURNAL OF ORGANIC CHEMISTRY   72 ( 14 )   5046 - 5055   2007.7

A new hydrophobic linker effective for the in situ synthesis of DNA-CPG conjugates as tools for SNP analysis Reviewed

Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Hiroshi Nagasawa, Toshifumi Tsukahara, Mitsuo Sekine

TETRAHEDRON LETTERS   48 ( 29 )   5147 - 5150   2007.7

Synthesis and hybridization properties of oligodeoxynucleotides incorporating 2-N-carbamoylguanine derivatives as guanine analogs Reviewed

Takeshi Sasami, Yoko Odawara, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

TETRAHEDRON LETTERS   48 ( 30 )   5325 - 5329   2007.7

Chemical properties of 4,5-di(ethoxycarbonyl)-1,3-dioxolan-2-yl (DECDO) as a hydroxyl protecting group of the 2 '-hydroxyl function in ribonucleosides Reviewed

Boleslaw Karwowski, Kohji Seio, Mitsuo Sekine

JOURNAL OF HETEROCYCLIC CHEMISTRY   44 ( 2 )   329 - 336   2007.3

Highly selective recognition of cytosine over uracil and adenine by a guanine analogue, 2-N-Acetyl-3-deazaguanine, in 2 '-O-Methyl-RNA/RNA and DNA duplexes

Kohji Seio, Takeshi Sasami, Akihiro Ohkubo, Kaori Ando, Mitsuo Sekine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   129 ( 5 )   1026 - 1027   2007.2

A pyrimidopyrimidoindole nucleoside (dC(PPI)): Photophysical properties and thermal stability of the modified DNA duplexes Reviewed

Masahiro Mizuta, Kohji Seio, Kenichi Miyata, Akihiro Ohkubo, Haruhiko Taguchi, Mitsuo Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   26 ( 10-12 )   1335 - 1338   2007

Development of an N-unprotected phosphoramidite method for the chemical synthesis of aminoacylated RNAs.

Akihiro Ohkubo, Yasuhiro Noma, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   1 - 2   2007

Recognition of triplex forming oligodeoxynucleotides incorporating abasic sites by 5-arylcytosine residues in duplex DNAs.

Masahiro Mizuta, Jun-Ichi Banba, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   25 - 26   2007

Development of a new method for the synthesis of oligodeoxynucleotides by use of carbamoyl-type protecting groups.

Akihiro Ohkubo, Rintaro Kasuya, Kenichi Miyata, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   131 - 132   2007

Recognition of target chain-length by use of oligonucleotides having bulky substituents at the terminal bases.

Yusuke Takaku, Masahiro Mizuta, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   283 - 284   2007

Synthesis and properties of oligodeoxynucleotides incorporating 5-carboxydeoxycytidine.

Masanori Sumino, Okamoto Itaru, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 51 )   165 - 166   2007

Short-step chemical synthesis of DNA by use of MMTrS group for protection of 5'-hydroxyl group.

Miyuki Shiraishi, Eri Utagawa, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

Nucleic acids symposium series (2004)   ( 51 )   129 - 130   2007

Synthesis and hybridization properties of oligodeoxynucleotides with long-chain linkers Reviewed

Kohji Seio, Takeshi Terada, Masahiro Mizuta, Akihiro Ohkubo, Haruhiko Taguchi, Mitsuo Sekine

HELVETICA CHIMICA ACTA   90 ( 10 )   1946 - 1965   2007

cis-tetrahydrofuran-3,4-diol structure as a key skeleton of new protecting groups removable by self-cyclization under oxidative conditions Reviewed

Eri Utagawa, Mitsuo Sekine, Kohji Seio

JOURNAL OF ORGANIC CHEMISTRY   71 ( 20 )   7668 - 7677   2006.9

Incorporation of 2 '-O-methyl-2-thiouridine into oligoribonucleotides induced stable A-form structure Reviewed

Okamoto, I, K Shohda, K Seio, M Sekine

CHEMISTRY LETTERS   35 ( 1 )   136 - 137   2006.1

Synthesis and biological properties of new phosmidosine analogs having an N-acylsulfamate linkage Reviewed

H. Taguchi, A. Ohkubo, M. Sekine, K. Seio, H. Kakeya, H. Osada, T. Sasaki

Nucleosides, Nucleotides and Nucleic Acids   25 ( 4-6 )   647 - 654   2006

A general method for the synthesis of 2 '-O-cyanoethylated oligoribonucleotides having promising hybridization affinity for DNA and RNA and enhanced nuclease resistance Reviewed

H Saneyoshi, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   70 ( 25 )   10453 - 10460   2005.12

Chemically stabilized phenylboranylidene groups having a dimethoxytrityl group as a colorimetrically detectable protecting group designed for cis-1,2-diol functions of ribonucleosides in the solid-phase synthesis of m(2)(2,2)G5 ' ppT Reviewed

M Sekine, M Aoyagi, M Ushioda, A Ohkubo, K Seio

JOURNAL OF ORGANIC CHEMISTRY   70 ( 21 )   8400 - 8408   2005.10

Computational evaluation of intermolecular interactions of a universal base 3-nitropyrrole in stacked dimers and DNA duplexes Reviewed

K Seio, H Ukawa, K Shohda, M Sekine

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS   22 ( 6 )   735 - 746   2005.6

Mild and facile deprotection for the synthesis of oligodeoxynucleotide incorporating a 6-O-ethyl-deoxyguanosine Reviewed

K Seio, T Negishi, K Negishi, M Sekine

LETTERS IN ORGANIC CHEMISTRY   2 ( 2 )   179 - 183   2005.3

Synthesis and hybridization properties of 2'-O-methyl-RNA incorporating 3-deazaguanine derivatives.

Kohji Seio, Takeshi Sasami, Akihiro Ohkubo, Mitsuo Sekine

Nucleic acids symposium series (2004)   ( 49 )   21 - 22   2005

Synthesis and Properties of New Nucleotide Analogues Possessing Squaramide Moieties as New Phosphate Isosters

SEIO Kohji, SEIO Kohji, MIYASHITA Takuhei, MIYASHITA Takuhei, SATO Kousuke, SEKINE Mitsuo, SEKINE Mitsuo

Eur. J. Org. Chem.   2005 ( 24 )   5163 - 5170   2005

[Development of new technologies directed toward superfine gene analysis].

Mitsuo Sekine, Akihiro Ohkubo, Itaru Okamoto, Akio Kobori, Kohji Seio

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   49 ( 17 Suppl )   2978 - 85   2004.12

Synthesis of benzodithiol-2-yl-substituted nucleoside derivatives as lead compounds having anti-bovine viral diarrhea virus activity Reviewed

K Seio, T Sasaki, K Yanagida, M Baba, M Sekine

JOURNAL OF MEDICINAL CHEMISTRY   47 ( 21 )   5265 - 5275   2004.10

Synthesis of enol adenosine 5 '-phosphate derivatives by the Perkow reaction of a silylated adenosine 5 '-phosphonate derivative with alpha-halo ketones Reviewed

T Moriguchi, K Okada, K Seio, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 3 )   246 - 248   2004.7

Synthesis and Structural Properties of New Oligonucleotide Analogues Containing a 2’,5’-Internucleotidic Squaryldiamide Linkage Capable of Formation of Watson-Crick and Wobble Base Pairs at the 3’-Downstream Junction Site

SATO K, TAWARADA R, SEIO K, SEKINE M

Eur. J. Org. Chem.   ( 10 )   2142 - 2150   2004

Synthesis of a biotin-conjugate of phosmidosine O-ethyl ester as a G1 arrest antitumor drug Reviewed

M. Sekine, K. Okada, K. Seio, T. Obata, T. Sasaki, H. Kakeya, H. Osada

Bioorganic and Medicinal Chemistry   12 ( 24 )   6343 - 6349   2004

Structure-activity relationship of phosmidosine: Importance of the 7,8-dihydro-8-oxoadenosine residue for antitumor activity Reviewed

M. Sekine, K. Okada, K. Seio, H. Kakeya, H. Osada, T. Sasaki

Bioorganic and Medicinal Chemistry   12 ( 19 )   5193 - 5201   2004

Synthesis of Chemically Stabilized Phosmidosine Analogues and the Structure-Activity Relationship of Phosmidosine Reviewed

M. Sekine, K. Okada, K. Seio, H. Kakeya, H. Osada, T. Obata, T. Sasaki

Journal of Organic Chemistry   69 ( 2 )   314 - 326   2004

New protected protecting groups for the 5 '-hydroxy group of deoxynucleosides by use of 2-(hydroxymethyl)- and 2-[(methylamino)methyl]benzoyl skeletons and oxidatively cleavable tritylthio and (4-methoxytrityl)thio groups Reviewed

K Seio, E Utagawa, M Sekine

HELVETICA CHIMICA ACTA   87 ( 9 )   2318 - 2333   2004

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab initio calculations.

Kohji Seio, Hisashi Ukawa, Koh-ichiro Shohda, Mitsuo Sekine

Nucleic acids research. Supplement (2001)   ( 3 )   49 - 50   2003

Synthesis and biological properties of stable phosmidosine analogs. Reviewed

K. Okada, H. Taguchi, K. Seio, H. Kakeya, H. Osada, T. Sasaki, M. Sekine

Nucleic acids research. Supplement (2001)   ( 3 )   105 - 106   2003

A convenient method for the conversion of beta-thymidine to alpha-thymidine based on TMSOTf-mediated C1 '-epimerization Reviewed

Y Sato, G Tateno, K Seio, M Sekine

TETRAHEDRON LETTERS   43 ( 17 )   3251 - 3254   2002.4

Synthesis and Conformational Properties of Dinucleoside Triester Derivatives Involving a Cyclonucleotide Bridge Structure Reviewed

Sekine M, Kurasawa O, Shoda K, Seio K, Wada T

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   1989 - 1999   2001

Synthesis and properties of a new oligonucleotide analogue containing an internucleotide squaryl amide linkage.

Sato K, Seio K, Sekine M

Nucleic acids symposium series   1   121 - 122   2001

A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside bases.

A. Ohkubo, K. Seio, M. Sekine

Nucleic acids research. Supplement (2001)   ( 1 )   77 - 78   2001

Fabrication of directly synthesized DNA chip using photolithography for rapid and parallel gene analysis Reviewed

K Takahashi, K Seio, M Sekine, O Hino, M Esashi

TRANSDUCERS '01: EUROSENSORS XV, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2   326 - 329   2001

Effects of methylene insertion in backbone of antisense oligonucleotides on RNase H mediated cleavage of mismatched duplexes

田平彩乃, 服部詩萌里, 清尾康志, 正木慶昭

日本核酸医薬学会年会講演要旨集(CD-ROM)   8th   2023

The effects of “X-CE”, modified nucleotides with various substituents via 2’-O-carbamoylethyl linker, on the reduction of toxicity and mRNA knockdown for Gapmer antisense oligonucleotides

入江雅彦, 細沢友紀, 西口由菜, 廣瀬優太, 加賀谷賢太, 日高夕貴, 窪山剛之, 正木慶昭, 清尾康志, 入山友輔

日本核酸医薬学会年会講演要旨集(CD-ROM)   8th   2023

Synthesis and properties of oligonucleotides containing 5 ́-deoxy-5 ́-hydroxymethylthymidine and 3 ́-deoxy-3 ́-hydroxymethylthymidine

正木慶昭, 正木慶昭, 田平彩乃, 服部詩萌里, 清尾康志

日本化学会春季年会講演予稿集(Web)   102nd   2022

Study of the synthesis of thymidine derivative whose base and 5 ́ position are linked through sulfonamide backbone

金川峻幸, 立花茂載, 大西里絵, 正木慶昭, 正木慶昭, 清尾康志

日本化学会春季年会講演予稿集(Web)   102nd   2022

Synthesis of Sulfonamide linkage-Containing Oligonucleotides and Evaluation of antisense activity

大西里絵, 清尾康志, 正木慶昭, 正木慶昭, 関谷彰太, 枝村亘

日本化学会春季年会講演予稿集(Web)   102nd   2022

Development of New Methods for Synthesis of Artificial Nucleic Acids having Various Functional Groups

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   72 ( 8 )   899 - 909   2014.8

Base recognition of gap sites in DNA－DNA and DNA－RNA duplexes by short oligonucleotides

Ken Yamada, Yohsuke Esaka, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Bioorganic & Medicinal Chemistry Letters   23 ( 11 )   3448 - 3451   2013

SYNTHESIS AND PROPERTIES OF OLIGONUCLEOTIDES HAVING 5 '-TERMINAL 2,2,7-TRIMETHYLGUANOSINE CAP ANALOGUES

Akihiro Ohkubo, Akira Yokouchi, Ken Yamada, Hirosuke Tsunoda, Kohji Seio, Mitsuo Sekine

NUCLEIC ACID THERAPEUTICS   21 ( 5 )   A39 - A40   2011.10

2′-O-[(N-メチルカルバモイル)エチル]ウリジンを組み込んだRNAオリゴマーの合成と性質

山田剛史, 岡庭夏己, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本化学会講演予稿集   91st ( 3 )   2011

2′水酸基修飾アンチセンスオリゴヌクレオチドの合成と筋ジストロフィーを対象としたエキソンスキッピング評価

山田剛史, 岡庭夏己, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本RNA学会年会要旨集   12th   2010

Development of a new strategy for the synthesis of oligonucleotides 5’-adenylated via a diphosphate linkage

大窪章寛, 西野雄大, 野間靖弘, 桑山靖和, 角田浩佑, 清尾康志, 関根光雄

日本化学会講演予稿集   89th ( 2 )   2009

プリンヌクレオシドへの2′-O-メチルカルバモイルエチル基の導入検討

山田剛史, 角田浩佑, 大窪章寛, 清尾康志, 関根光雄

日本RNA学会年会要旨集   11th   2009

Binding of MutS protein to oligonucleotides containing a methylated- or an ethylated guanine residue, and correlation with mutation frequency

Kentro Taira, Shintaro Nakamura, Khota Nakano, Daisuke Maehara, Keinosuke Okamoto, S. Arimoto, D. Loakes, L. Worth, Jr, R. M. Schaaper, Kohji Seio, Mitsuo Sekine, Kazuo Negishi, Tomoe Negishi

Mutation Research   640   107 - 112   2008

Synthesis and fluorescent properties of bi- and tricyclic 4-N-carbamoyldeoxycytidine derivatives

K.Miyata, R.Mineo, R.Tamamushi, M.Mizuta, A.Ohkubo, H.Taguchi, K.Seio, T.Santa

J Org Chem   72 ( 1 )   102 - 108   2007

2’末端に極性をもった官能基を導入した修飾核酸の合成と性質

山田剛史, 山田剛史, 實吉尚郎, 大窪章寛, 大窪章寛, 清尾康志, 清尾康志, 関根光雄, 関根光雄

アンチセンスシンポジウム講演要旨集   17th   2007

1,1-Dihydroperoxycyclododecaneas a new, crystalline non-hygroscopic oxidizer for the chemical synthesis of oligodeoxyribonucleotides

Hisao Saneyoshi, Kenichi Miyata, Kohji Seio, Mitsuo Sekine

TETRAHEDRON LETTERS   47 ( 50 )   8945 - 8947   2006.12

Synthesis of 2 '-O-methyl-RNAs incorporating a 3-deazaguanine, and UV melting and computational studies on its hybridization properties

Kohji Seio, Takeshi Sasami, Ryuya Tawarada, Mitsuo Sekine

NUCLEIC ACIDS RESEARCH   34 ( 16 )   4324 - 4334   2006.9

Conformational studies of 4-N-carbamoyldeoxycytidine derivatives and synthesis and hybridization properties of oligodeoxyribonucleotides incorporating these modified bases

Kenichi Miyata, Akio Kobori, Ryuji Tamamushi, Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   2006 ( 16 )   3626 - 3637   2006.8

Improved synthesis of oligonucleotides containing 2-thiouridine derivatives by use of diluted iodine solution

Okamoto, I, K Seio, M Sekine

TETRAHEDRON LETTERS   47 ( 4 )   583 - 585   2006.1

DNA Synthesis without Base Protection Using the Phosphoramidite Approach

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Current Protocol in Nucleic Acid Chemistry   1 ( 3.15 )   1 - 20   2006

Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative

K.Miyata, R.Tamamushi, A.Ohkubo, H.Taguchi, K.Seio, T.Santa, M.Sekine

Org Lett   8 ( 8 )   1545 - 1548   2006

Synthesis of Deoxycytidine Derivative and Their Use for the Selective Photo Crosslinking with 5-Methylcytosine

Haruhiko Taguchi, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   57 - 58   2006

Synthesis and Properties of Artificial Base Pairs by Use of Halogen Bonds

Ryuta Tawarada, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   121 - 122   2006

Synthesis and Properties of DNA Oligomers Containing 2'-Deoxycytidine-N-Oxide

Hirosuke Tsunoda Akihiro Ohkubo, Haruhiko Taguchi, Kohji Seio, Mitsuo Sekine

Nucleic Acids Res. Symposium Ser.   50   189 - 190   2006

Use of ferrocene scaffolds as pendant groups in hairpin-type pyrrole-imidazole polyamide molecules showing sequence-selective binding to DNA duplexes

K Seio, M Mizuta, T Terada, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   70 ( 25 )   10311 - 10322   2005.12

Synthesis of oligodeoxyribonucleotides containing hydroxymethylphosphonate bonds in the phosphoramidite method and their hybridization properties

A Ohkubo, K Aoki, Y Ezawa, Y Sato, H Taguchi, K Seio, M Sekine

TETRAHEDRON LETTERS   46 ( 51 )   8953 - 8957   2005.12

Convenient synthesis of N-unprotected deoxynucleoside 3 '-phosphoramidite building blocks by selective deacylation of N-acylated species and their facile conversion to other N-functionalized derivatives

A Ohkubo, K Sakamoto, K Miyata, H Taguchi, K Seio, M Sekine

ORGANIC LETTERS   7 ( 24 )   5389 - 5392   2005.11

Synthesis and conformational analysis of 4-N-carbamoyldeoxycytidine derivatives.

K Miyata, R Tamamushi, A Ohkubo, H Taguchi, K Seio, M Sekine

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   229   U560 - U560   2005.3

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method.

A Ohkubo, H Taguchi, K Seio, M Sekine

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   229   U559 - U560   2005.3

4,5-bis(ethoxycarbonyl)-[1,3]dioxolan-2-yl as a new orthoester-type protecting group for the 2 '-hydroxyl function in the chemical synthesis of RNA

B Karwowski, K Seio, M Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   24 ( 5-7 )   1111 - 1114   2005

Analysis by Use of Protected Oligonucleotides Probes.

A. Ohkubo, R. Kasuya, K. Sakamoto, H. Taguchi, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   19 - 20   2005

A new protecting group for the 5 '-hydroxyl group having O-S single bond oxidatively cleavable under mild conditions

E Utagawa, K Seio, M Sekine

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   24 ( 5-7 )   927 - 929   2005

Development of New N-Unprotected Phosphoramidite Building Blocks Having a Silyl-Type Linker

A. Ohkubo, K. Aoki, H. Taguchi, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   127 - 128   2005

Synthesis of 2'- or 3'-O-Heteroaryl Substituted Nucleic Acid Derivatives and Their Biological Properties

H. Taguchi, T. Narita, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   113 - 114   2005

SNPs Analysis by Use of Oligonucleotides Containing 2'-O-Methyl-2-thiouridines.

I. Okamoto, K. Seio, M. Sekine

Nucleic Acids Symposium Ser.   49   123 - 124   2005

Synthesis and hybridization affinity of oligodeoxyribonucleotides incorporating 4-N-(N-arylcarbamoyl)deoxycytidine derivatives

K Miyata, R Tamamushi, A Ohkubo, H Taguchi, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 51 )   9365 - 9368   2004.12

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method

A Ohkubo, Y Ezawa, K Seio, M Sekine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   126 ( 35 )   10884 - 10896   2004.9

Synthesis and properties of a pyrrole-imidazole polyamide having a ferrocene dicarboxylic amide linker

K Seio, M Mizuta, T Terada, M Sekine

TETRAHEDRON LETTERS   45 ( 36 )   6783 - 6786   2004.8

Fine-tuning of acid susceptibility of 4,4 '-dimethoxytrityl ether derivatives by a methoxy group introduced via a styryl substituent

K Seio, T Sasaki, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 2 )   159 - 162   2004.4

Synthesis and stability of 1-phenylethenyl phosphate derivatives and their phosphoryl transfer activity

T Moriguchi, K Okada, K Seio, M Sekine

LETTERS IN ORGANIC CHEMISTRY   1 ( 2 )   140 - 144   2004.4

A new approach for pyrophosphate bond formation starting from phosphoramidite derivatives by use of 6-trifluoromethyl-1-hydroxybenzotriazole-mediated O-N phosphoryl migration

A Ohkubo, K Aoki, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 5 )   979 - 982   2004.1

A new strategy for the synthesis of oligodeoxynucleotides directed towards perfect O-selective internucleotidic bond formation without base protection

A Ohkubo, K Seio, M Sekine

TETRAHEDRON LETTERS   45 ( 2 )   363 - 366   2004.1

Synthesis and Base Recognition Ability of Oligoribonucleotides Incorporating 2-O-methyl-3-Deazaguanosine

Kohji Seio, Takeshi, Sasami Mitsuo Sekine

Nucleic Acids Symp. Series   48   65 - 66   2004

Properties of Ferrocene-polyamide Compounds as Redox Active DNA binding Molecules toward SNPs Detection

Masahiro Mizuta, Takeshi Terada, Kohji Seio, Mitsuo Sekine

Nucleic Acids Symp. Series   48   237 - 238   2004

A new route to 2 '-O-alkyl-2-thiouridine derivatives via 4-o-protection of the uracil base and hybridization properties of oligonucleotides incorporating these modified nucleoside derivatives

Okamoto, I, K Shohda, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   68 ( 26 )   9971 - 9982   2003.12

Proton-block strategy for the synthesis of oligodeoxynucleotides without base protection, capping reaction, and P-N bond cleavage reaction

M Sekine, A Ohkubo, K Seio

JOURNAL OF ORGANIC CHEMISTRY   68 ( 14 )   5478 - 5492   2003.7

Enhanced stereoselectivity in internucleotidic bond formation by the use of the chiral ribose moiety of thymidine

K Seio, K Kumura, JC Bologna, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   68 ( 10 )   3849 - 3859   2003.5

Synthesis of TMG-capped RNA-DNA chimeric oligonucleotides

M Sekine, M Ushioda, T Wada, K Seio

TETRAHEDRON LETTERS   44 ( 8 )   1703 - 1707   2003.2

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab intio calculations

Kohji Seio, Hisashi Ukawa, Koh-ichiro Shohda, Mitsuo Sekine

Nucleic Acids Res. Suppl.   3   49 - 50   2003

Enhanced Stereoselectivity in Internucleotide Linkage Formation by the Effective Use of Intrinsically Chiral Ribose Miety of Nucleoside.

K. Seio, K. Kumura, M. Sekine

J. Org. Chem.   68   3849 - 3859   2003

A Proton-Block Strategy in the Phosphoramidite Approach: A New N-Unprotected Method for the Synthesis of Oligodeoxynucleotides Based on the Principle of Simple Acid-Base Reactions.

M. Sekine, A. Ohkubo, K. Seio

J. Org. Chem.   68   5478 - 5492   2003

Squaryl group as a new mimic of phosphate group in modified oligodeoxynucleotides: Synthesis and properties of new oligodeoxynucleotide analogues containing an internucleotidic squaryldiamide linkage

K Sato, K Seio, M Sekine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   124 ( 43 )   12715 - 12724   2002.10

First synthesis and anticancer activity of phosmidosine and its related compounds

T Moriguchi, N Asai, K Okada, K Seio, T Sasaki, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   67 ( 10 )   3290 - 3300   2002.5

A photochemical/chemical direct method of synthesizing high-performance deoxyribonucleic acid chips for rapid and parallel gene analysis

K Takahashii, K Seio, M Sekine, O Hino, M Esashi

SENSORS AND ACTUATORS B-CHEMICAL   83 ( 1-3 )   67 - 76   2002.3

Substituent and solvent effects of TMS triflate mediated C1 ' epimerization of beta-thymidine to alpha-thymidine

Y Sato, G Tateno, K Seio, M Sekine

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   ( 1 )   87 - 93   2002.1

A new method for the synthesis of oligodeoxyribonucleotides containing 4-N-alkoxycarbonyldeoxycytidine derivatives and their hybridization properties

A Kobori, K Miyata, M Ushioda, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   67 ( 2 )   476 - 485   2002.1

A new silyl ether-type linker useful for the automated synthesis of oligonucleotides having base-labile protective groups

A Kobori, K Miyata, M Ushioda, K Seio, M Sekine

CHEMISTRY LETTERS   ( 1 )   16 - 17   2002.1

Unique participation of unprotected internucleotidic phosphodiester residues on unexpected cleavage reaction of the Si-O bond of the diisopropylsilandiyl group used as a linker for the solid-phase synthesis of 5 '-terminal guanylated oligodeoxynucleotides

M Ushioda, M Kadokura, T Moriguchi, A Kobori, M Aoyagi, K Seio, M Sekine

HELVETICA CHIMICA ACTA   85 ( 9 )   2930 - 2945   2002

A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates

Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   29 - 30   2002

A new protecting group for 5'-hydroxyl function of nucleitides in oligonucleotide snthesis without acid treatment utilizing unique properties of tritylthio group

Kohji Seio Eri Utagawa, Mitsuo Sekine

Nucleic Acid Research Supplement   2   27 - 28   2002

A new protecting group for 5'-hydroxyl function of nucleotides in oligonucleotide synthesis without acid treatment utilizing unique properties of tritylthio group

Kohji Seio Eri Utagawa, Mitsuo Sekine

Nucleic Acids Research Supplement   2   27 - 28   2002

A New Finding in Connection with Side Reaction Associated with 5'-Terminal Pyrophosphorylation of Oligodeoxynucleotides on Polymer Supports: Selective Si-O Bond Cleavage by Strong Nucleophilicity of 4-Nitro-6-(triluoromethyl)-1H-1,2,3-benzotriazol-1-ol

M. Ushioda M, Kadokura, T. Moriguchi, A. Kobori, M. Aoyagi, K. Seio, M. Sekine

Helv. Chim. Acta.   85   2930 - 2945   2002

Computational Analysis of Stacking Interactions betwee 3-Nitropyrrole and Natural Nucleobases

Hisashi Ukawa, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   173 - 174   2002

Hybridization Ability and Base Pair Geometry of Modified Deoxycytidine Derivatives Having a 4-N-Carbamoyl Group

Kenichi Miyata Akio Kobori, Kohji Seio, Mitsuo Sekine

Nucleic Acids Research Supplement   2   161 - 162   2002

Solid-phase synthesis of a 5 '-terminal TMG-capped trinucleotide block of U1 snRNA

M Kadokura, T Wada, K Seio, T Moriguchi, J Huber, R Luhrmann, M Sekine

TETRAHEDRON LETTERS   42 ( 50 )   8853 - 8856   2001.12

Synthesis of pentathymidylate using a 4-monomethoxytritylthio (MMTrS) group as a 5 '-hydroxyl protecting group: toward oligonucleotide synthesis without acid treatment

K Seio, M Sekine

TETRAHEDRON LETTERS   42 ( 49 )   8657 - 8660   2001.12

Total synthesis of agrocin 84 and phosmidosine as naturally occurring nucleotidic antibiotics having P-N bond linkages

M Sekine, T Moriguchi, T Wada, K Seio

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN   59 ( 11 )   1109 - 1120   2001.11

Essential factors for stabilization of the predominant C3 '-endo conformation in dinucleoside phosphotriester derivatives with cyclonucleotide bridge structures at the downstream 3 '-position

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY   ( 10 )   1989 - 1999   2001.5

Binding Specificities of the Mismatch Binding Protein, MutS, to Oligonucleotides Containing Modified bases

K. Negishi, D. Maehara, S. Nakamura, D. Loakes, l. Worth, Jr. R. M. Schaaper, K. Seio, M, Sekine T. Negishi

Nuceic Acids Research Supplement No1   221 - 222   2001

Synthesis and Properties of a New Nucleotide Analogue Containing Squaryl Group

Kousuke Sato, Kohji Seio, Mitsuo Sekine

Nuceic Acids Research Supplement No1   121 - 122   2001

A New Coupling Method for the Synthesis of Oligodeoxyribonucleotides Based on Protection by Protonation of Nnucleoside Bbases

A. Ohkubo, K. Seio, M. Sekine

Nuceic Acids Research Supplement No1   77 - 78   2001

Substituent and Solvent Effects of TMS-triflate mediated C1'-Epimerization of beta-Thymidine to alpha-Thymidine

Yuichi Sato, Gohsuke, Tateno, Kohji Seio, Mitsuo Sekine

Eur. J. Org. Chem.   4583 - 4593   2001

Synthesis and properties of oligonucleotides having a phosphorus chiral center by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

JOURNAL OF ORGANIC CHEMISTRY   65 ( 20 )   6515 - 6524   2000.10

Synthesis and properties of 2 '-O-methyl-2-thiouridine and oligoribonucleotides containing 2 '-O-methyl-2-thiouridine

K Shohda, Okamoto, I, T Wada, K Seio, M Sekine

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   10 ( 16 )   1795 - 1798   2000.8

Synthesis of 4-thiouridine, 6-thioinosine, and 6-thioguanosine 3 ',5 '-O-bisphosphates as donor molecules for RNA ligation and their application to the synthesis of photoactivatable TMG-capped U1 snRNA fragments

M Kadokura, T Wada, K Seio, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   65 ( 17 )   5104 - 5113   2000.8

Synthesis and antitumor activities of phosmidosine A and its N- acetylated derivative

Tomohisa Moriguchi, Norio Asai, Takeshi Wada, Kohji Seio, Takuma Sasaki, Mitsuo Sekine

Tetrahedron Letters   41 ( 31 )   5881 - 5885   2000.7

Synthesis and properties of oligodeoxynucleotides incorporating a conformationally rigid uridine unit having a cyclic structure at the 5 '-terminal site

M Sekine, O Kurasawa, K Shohda, K Seio, T Wada

JOURNAL OF ORGANIC CHEMISTRY   65 ( 12 )   3571 - 3578   2000.6

Synthesis and properties of oligothymidylates incorporating an artificial bend motif

K Seio, T Wada, M Sekine

HELVETICA CHIMICA ACTA   83 ( 1 )   162 - 180   2000

Chemical synthesis of hydroxymethylphosophnate α-DNA

KOHJI SEIO

Nucleic Acids Sym. Ser.   44   31 - 32   2000

Synthesis of N-labeled peptidyl AMP

H Narita, T Moriguchi, K Seio, M Sekine, H Miyaguchi, K Sakamoto, S Yokoyama

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   19 ( 10-12 )   1993 - 2003   2000

Solid-phase synthesis of deoxyribonucleotides having a cap structure analog

KOHJI SEIO

Nucleic Acids Symp. Ser.   44   123 - 124   2000

Synthesis of artificially bent oligonucleotides by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Seio, K Shohda, T Wada

NUCLEOSIDES & NUCLEOTIDES   18 ( 6-7 )   1163 - 1168   1999

Synthesis of artificially bent oligonucleotides by incorporation of conformationally rigid 5 '-cyclouridylic acid derivatives

M Sekine, O Kurasawa, K Seio, K Shohda, T Wada

NUCLEOSIDES & NUCLEOTIDES   18 ( 6-7 )   1163 - 1168   1999

The Chemical Synthesis of Hypermethylated Capped RNA on Polymer Supports

M. Sekine, M, Kadokura T, Satoh T, Wada K. Seio

J. Mol. Science   15   318   1999

Chemical synthesis and properties of conformationally fixed diuridine monophosphates as building blocks of the RNA turn motif

K Seio, T Wada, K Sakamoto, S Yokoyama, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   63 ( 5 )   1429 - 1443   1998.3

Synthesis and properties of conformationally rigid cyclouridylic acids having covalent bonding linkers between the uracil 5-position and the 5'-phosphate group

K Seio, O Kurasawa, T Wada, K Sakamoto, S Yokoyama, M Sekine

NUCLEOSIDES & NUCLEOTIDES   16 ( 7-9 )   1023 - 1032   1997

Chemical synthesis of a 5'-terminal TMG-capped triribonucleotide m(3)(2,2,7)G(5')pppAmpUmpA of U1 RNA

M Sekine, M Kadokura, T Satoh, K Seio, T Wada, U Fischer, Sumpter, V, R Luhrmann

JOURNAL OF ORGANIC CHEMISTRY   61 ( 13 )   4412 - 4422   1996.6

Chemical synthesis and conformational properties of a new cyclouridylic acid having an ethylene bridge between the uracil 5-position and 5'-phosphate group

K Seio, T Wada, K Sakamoto, S Yokoyama, M Sekine

JOURNAL OF ORGANIC CHEMISTRY   61 ( 4 )   1500 - 1504   1996.2

Synthesis and properties of conformationally rigid cyclouridylic acid having a covalent bonding linker between the uracil 5-position and the 5′-phosphate group

Kohji Scio, Takeshi Wada, Kensaku Sakamoto, Shigeyuki Yokoyama, Mitsuo Sekine

Tetrahedron Letters   36 ( 52 )   9515 - 9518   1995.12

Synthesis of oligonucleotide having a bent structure by incorporation of an interresidually cyclized uridylyl(3'-5')uridine unit

KOHJI SEIO

Nucleic Acids Symp. Ser.   34   181 - 182   1995

CHEMICAL SYNTHESIS AND PROPERTIES OF AN INTERRESIDUALLY CYCLIZED URIDYLYL(3'-5')URIDINE AS A MODEL OF TRANSFER-RNA U-TURN STRUCTURE HAVING A SHARP BEND

M SEKINE, K SEIO, T WADA, K SAKAMOTO, S YOKOYAMA

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   116 ( 10 )   4469 - 4470   1994.5

Chemical synthesis of the 5'-terminal structure of U1 RNA

KOHJI SEIO

Nucleic Acids Symp. Ser.   31   61 - 62   1994

SYNTHESIS AND PROPERTIES OF N-TRITYLTHIO NUCLEOSIDE DERIVATIVES AND REDUCTIVE REMOVAL OF THE TRITYLTHIO GROUP BY USE OF TRIBUTYLTIN HYDRIDE AND TRIS(TRIMETHYLSILYL)SILANE

M SEKINE, K SEIO

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1   ( 24 )   3087 - 3093   1993.12

SYNTHESIS OF URIDYLYL (3'-5') URIDINE DERIVATIVES CONTAINING 5-(METHYLAMINO-METHYL)URIDINE AS A MODIFIED NUCLEOSIDE FOUND FROM ESCHERICHIA-COLI MINOR TRANSFER RNA(ARG)

M SEKINE, K SEIO, T SATOH, K SAKAMOTO, S YOKOYAMA

NUCLEOSIDES & NUCLEOTIDES   12 ( 3-4 )   305 - 321   1993

Enzymatic synthesis of site specifically isotope labelled RNA

Grant number：15K13738  2015.4 - 2017.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

Seio Kohji

Chemically modified DNAs cotrolled by triplex forming oligodeoxynucleotides

Grant number：26288075  2014.4 - 2017.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

Seio Kohji

DNA-タンパク質相互作用の光制御を利用した遺伝子回路への光分子スイッチの導入

Grant number：26119705  2014.4 - 2016.3

日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

清尾 康志

New miRNA detecting system utilizing fluorescent DNA triplexes and rolling circle amplification

Grant number：25620126  2013.4 - 2015.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

SEIO KOHJI

Development of miRNA detection techniques using artificial nucleic acids capable of short RNA selective ginding

Grant number：23350077  2011.11 - 2014.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

SEIO Kohji

Development of T7 promoter controlled by DNA triplex formation

Grant number：23655154  2011

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

SEIO Kohji

Detection of miRNAs using artificial oligonucleotide probes capable of chain length discrimination

Grant number：20350074  2008 - 2010

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

SEIO Kohji

Large Scaled Fast Aqueous Computing with Light Responsive DNA upon Micro Fluidic Devices

Grant number：17200022  2005 - 2006

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

YAMAMURA Masayuki, SEIO Koji, FUJII Teruo, FUJIMOTO Kenzo

電気化学的モレキュラービーコンによる高速遺伝子検出法の開発

Grant number：16651060  2004

日本学術振興会  科学研究費助成事業  萌芽研究

清尾 康志

ゲノム修復型遺伝子治療を指向した、機能性DNAドラッグのデザインと創製

Grant number：15012218  2003

日本学術振興会  科学研究費助成事業  特定領域研究

清尾 康志

EXPLORATION OF ULTIMATE NEW SYNTHETIC METHOD OF DNA/RNA BY USE OF "PROTON-BLOCK APPROACH"-DIRECTED TOWARD ESTABLISHMENT OF INOVATIVE METHOD FOR THE SYNTHESIS OF NUCLEIC ACIDS WITHOUT BASE PROTECTION-

Grant number：14208074  2002 - 2003

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

SEKINE Mitsuo, SEIO Khoji

ミスマッチ塩基対安定化能を有する人工DNAのデザインと開発

Grant number：14011218  2002

日本学術振興会  科学研究費助成事業  特定領域研究

清尾 康志

塩基配列を精密に認識するインテリジェント核酸プローブのデザインと開発

Grant number：13202021  2001

日本学術振興会  科学研究費助成事業  特定領域研究(C)

清尾 康志

核酸の新規化学合成法の開発とその応用

Developping new approaches toward the chamical synthesis of DNA and RNA.