Updated on 2026/07/01

写真a

 
NAKAMURA NOBUHIRO
 
Organization
School of Life Science and Technology Associate Professor
Title
Associate Professor
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Research Interests

  • 細胞生物学

  • 生化学一般

  • Biochemistry in general

  • 分子生物学

Research Areas

  • Life Science / Molecular biology

  • Life Science / Cell biology

Education

  • Tokyo Institute of Technology   Bioscience and Biotechnology   Biological Sciences

    - 2003

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    Country: Japan

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  • Tokyo Institute of Technology   Graduate School of Bioscience and Biotechnology   Department of Biological Sciences

    - 2003

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  • Tokyo Institute of Technology   School of Bioscience and Biotechnology

    - 1998

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    Country: Japan

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Research History

  • Tokyo Institute of Technology   Associate Professor

    2010.10

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  • 東京工業大学 大学院生命理工学研究科 助手

    2003.4 - 2010.9

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  • 日本学術振興会 特別研究員

    2000.4 - 2003.3

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Professional Memberships

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Papers

  • Adhesion G protein-coupled receptor F5 mediates shear stress mechanotransduction in glomerular endothelial cells Reviewed

    Xiaoyang Wang, Masako Kato, Miki Nagase, Nobuhiro Nakamura

    The Journal of Biochemistry   2026.6

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Adhesion G protein-coupled receptor F5 (ADGRF5) is widely expressed and is involved in various physiological and pathophysiological functions. ADGRF5 has been demonstrated to be expressed in glomerular endothelial cells and to maintain the integrity of the glomerular filtration barrier by regulating the expression of type IV collagens COL4A3 and COL4A4. However, the mechanisms underlying its activation remain unclear. Herein, we showed that ADGRF5 overexpression enhances ERK1/2 phosphorylation induced by laminar flow shear stress in human embryonic kidney PEAKrapid cells. Inhibitor and knockdown experiments revealed that the ADGRF5-mediated shear stress response proceeds via the Gαq/11–protein kinase C–RAF1–MEK1/2–ERK1/2 cascade. Mutagenesis analyses showed that shear flow-mediated activation of ADGRF5 requires the tethered agonist (Stachel) sequence and the C-terminal helix 8. Consistent with the overexpression analyses, endogenous ADGRF5 mediated shear stress-induced ERK1/2 signalling in human primary renal glomerular endothelial cells. Moreover, ADGRF5 knockdown attenuated shear flow-induced upregulation of the immediate early response genes and the type IV collagen genes COL4A3 and COL4A4. These results suggest that ADGRF5 mediates shear stress mechanotransduction in glomerular endothelial cells and regulates endothelial cell responses, with potential implications for glomerular filtration barrier homeostasis.

    DOI: 10.1093/jb/mvag039

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  • Podocyte-specific deletion of mechanochannel Piezo1 exacerbates proteinuria and podocyte injury in mouse hypertensive nephropathy Reviewed

    Kaori Mikami, Takashi Nagase, Hisaki Kishino, Nobuhiro Nakamura, Yoshiro Suzuki, Hiroshi Fukuhara, Taiji Matsusaka, Keiko Nonomura, Miki Nagase

    HYPERTENSION RESEARCH   49 ( 2 )   286 - 302   2026.2

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    DOI: 10.1038/S41440-025-02383-W

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  • Glomerular Endothelial Cell Receptor Adhesion G-Protein–Coupled Receptor F5 (ADGRF5) and the Integrity of the Glomerular Filtration Barrier Reviewed

    Miki Nagase, Hikaru Ando, Yoshiaki Beppu, Hidetake Kurihara, Souta Oki, Fumimasa Kubo, Kazuki Yamamoto, Takashi Nagase, Shinya Kaname, Yoshihiro Akimoto, Hiroshi Fukuhara, Tatsuo Sakai, Shigehisa Hirose, Nobuhiro Nakamura

    Journal of the American Society of Nephrology   35 ( 10 )   1366 - 1380   2024.6

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Ovid Technologies (Wolters Kluwer Health)  

    Key Points

    Deletion of endothelial receptor adhesion G-protein–coupled receptor F5 in mice led to abnormal structural and functional properties of the glomerular filtration barrier.

    Adhesion G-protein–coupled receptor F5 regulates gene expression of glomerular basement membrane components and a mechanosensitive transcription factor.



    Background

    Glomerular endothelial cells are recognized to be important for maintaining the glomerular filtration barrier. Adhesion G-protein–coupled receptor F5 (ADGRF5), an adhesion G protein–coupled receptor, has been suggested to be involved in endothelial cell function. However, the role of ADGRF5 in the glomerular filtration barrier integrity remains elusive.

    Methods

    Cellular expression of ADGRF5 in mouse glomerulus was determined by histological analyses. The effect of ADGRF5 deletion on the glomerular morphology, kidney function, and glomerular endothelial gene/protein expression was then analyzed using ADGRF5 knockout (Adgrf5 −/−) mice and human primary glomerular endothelial cells.

    Results

    ADGRF5 was specifically expressed in the capillary endothelial cells within the glomerulus. Adgrf5 −/− mice developed albuminuria and impaired kidney function with morphological defects in the glomeruli, namely glomerular hypertrophy, glomerular basement membrane splitting and thickening, diaphragmed fenestration and detachment of the glomerular endothelial cells, and mesangial interposition. These defects were accompanied by the altered expression of genes responsible for glomerular basement membrane organization (type 4 collagens and laminins) and Krüppel-like factor 2 (Klf2) in glomerular endothelial cells. Moreover, ADGRF5 knockdown decreased COL4A3 and COL4A4 expression and increased KLF2 expression in human primary glomerular endothelial cells.

    Conclusions

    The loss of ADGRF5 resulted in altered gene expression in glomerular endothelial cells and perturbed the structure and permselectivity of the glomerular filtration barrier.

    DOI: 10.1681/asn.0000000000000427

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  • Carbonic anhydrase inhibitor induces otic hair cell apoptosis via an intrinsic pathway and ER stress in zebrafish larvae Reviewed

    Hiroko Matsumoto, Hisako Miyagi, Nobuhiro Nakamura, Yasuhiro Shiga, Toshihiro Ohta, Shoko Fujiwara, Mikio Tsuzuki

    Toxicology Reports   8   1937 - 1947   2021.11

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    DOI: 10.1016/j.toxrep.2021.11.018

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  • Orphan GPR116 mediates the insulin sensitizing effects of the hepatokine FNDC4 in adipose tissue. Reviewed

    Nobuhiro Nakamura

    Nature communications   12   2999   2021.5

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    The proper functional interaction between different tissues represents a key component in systemic metabolic control. Indeed, disruption of endocrine inter-tissue communication is a hallmark of severe metabolic dysfunction in obesity and diabetes. Here, we show that the FNDC4-GPR116, liver-white adipose tissue endocrine axis controls glucose homeostasis. We found that the liver primarily controlled the circulating levels of soluble FNDC4 (sFNDC4) and lowering of the hepatokine FNDC4 led to prediabetes in mice. Further, we identified the orphan adhesion GPCR GPR116 as a receptor of sFNDC4 in the white adipose tissue. Upon direct and high affinity binding of sFNDC4 to GPR116, sFNDC4 promoted insulin signaling and insulin-mediated glucose uptake in white adipocytes. Indeed, supplementation with FcsFNDC4 in prediabetic mice improved glucose tolerance and inflammatory markers in a white-adipocyte selective and GPR116-dependent manner. Of note, the sFNDC4-GPR116, liver-adipose tissue axis was dampened in (pre) diabetic human patients. Thus our findings will now allow for harnessing this endocrine circuit for alternative therapeutic strategies in obesity-related pre-diabetes.

    DOI: 10.1038/s41467-021-22579-1

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  • Cigarette smoke attenuates p300-mediated Nrf2 acetylation in macrophages: Is stabilizing Nrf2 enough to halt COPD progression? Invited Reviewed

    Nobuhiro Nakamura

    RESPIROLOGY   26 ( 1 )   19 - 20   2021.1

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    DOI: 10.1111/RESP.13910

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  • ppGpp functions as an alarmone in metazoa Reviewed

    Doshun Ito, Hinata Kawamura, Akira Oikawa, Yuta Ihara, Toshio Shibata, Nobuhiro Nakamura, Tsunaki Asano, Shun-Ichiro Kawabata, Takashi Suzuki, Shinji Masuda

    Communications Biology   3 ( 1 )   671   2020.12

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    DOI: 10.1038/s42003-020-01368-4

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  • Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells Reviewed

    Nobuhiro Nakamura

    RESPIRATORY RESEARCH   20 ( 1 )   2019.12

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    DOI: 10.1186/S12931-019-0973-6

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  • Ubiquitin System Invited Reviewed

    Nobuhiro Nakamura

    International Journal of Molecular Sciences   19 ( 4 )   1080 - 1080   2018.4

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:{MDPI} {AG}  

    DOI: 10.3390/ijms19041080

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  • Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear Reviewed

    Hiroko Matsumoto, Shoko Fujiwara, Hisako Miyagi, Nobuhiro Nakamura, Yasuhiro Shiga, Toshihiro Ohta, Mikio Tsuzuki

    MARINE BIOTECHNOLOGY   19 ( 5 )   430 - 440   2017.10

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    DOI: 10.1007/s10126-017-9763-7

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  • USP19-Mediated Deubiquitination Facilitates the Stabilization of HRD1 Ubiquitin Ligase. Reviewed

    Harada, Kumi, Kato, Masako, Nakamura, Nobuhiro

    International journal of molecular sciences   17 ( 11 )   2016

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    DOI: 10.3390/ijms17111829

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  • Characterization of the zebrafish cx36.7 gene promoter: Its regulation of cardiac-specific expression and skeletal muscle-specific repression. Reviewed

    Miyagi, Hisako, Nag, Kakon, Sultana, Naznin, Munakata, Keijiro, Hirose, Shigehisa, Nakamura, Nobuhiro

    Gene   577 ( 2 )   265 - 274   2016

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    DOI: 10.1016/j.gene.2015.12.013

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  • Targeted Disruption of Ig-Hepta/Gpr116 Causes Emphysema-like Symptoms That Are Associated with Alveolar Macrophage Activation Reviewed

    Ariestanti, Donna Maretta, Ando, Hikaru, Hirose, Shigehisa, Nakamura, Nobuhiro

    Journal of Biological Chemistry   290 ( 17 )   11032 - 11040   2015

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    DOI: 10.1074/jbc.M115.648311

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  • Ligand-induced internalization, recycling, and resensitization of adrenomedullin receptors depend not on CLR or RAMP alone but on the receptor complex as a whole Reviewed

    Nag, Kakon, Sultana, Naznin, Kato, Akira, Dranik, Anna, Nakamura, Nobuhiro, Kutsuzawa, Koichi, Hirose, Shigehisa, Akaike, Toshihiro

    General and Comparative Endocrinology   212   156 - 162   2015

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    DOI: 10.1016/j.ygcen.2014.04.029

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  • Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6 Reviewed

    Nakamura, Nobuhiro, Harada, Kumi, Kato, Masako, Hirose, Shigehisa

    Experimental Cell Research   328 ( 1 )   207 - 216   2014

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    DOI: 10.1016/j.yexcr.2014.07.025

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  • Close association of carbonic anhydrase (CA2a and CA15a), Na+/H+ exchanger (Nhe3b), and ammonia transporter Rhcg1 in zebrafish ionocytes responsible for Na+ uptake Reviewed

    Ito, Yusuke, Kobayashi, Sayako, Nakamura, Nobuhiro, Miyagi, Hisako, Esaki, Masahiro, Hoshijima, Kazuyuki, Hirose, Shigehisa

    Frontiers in Physiology   4   2013

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    DOI: 10.3389/fphys.2013.00059

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  • Identification and properties of a novel variant of NBC4 (Na+/HCO3- co-transporter 4) that is predominantly expressed in the choroid plexus Reviewed

    Fukuda, Hidekazu, Hirata, Taku, Nakamura, Nobuhiro, Kato, Akira, Kawahara, Katsumasa, Wakabayashi, Shigeo, Chang, Min-Hwang, Romero, Michael F., Hirose, Shigehisa

    Biochemical Journal   450   179 - 187   2013

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    DOI: 10.1042/BJ20121515

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  • Lung Surfactant Levels are Regulated by Ig-Hepta/GPR116 by Monitoring Surfactant Protein D Reviewed

    Fukuzawa, Taku, Ishida, Junji, Kato, Akira, Ichinose, Taro, Ariestanti, Donna Maretta, Takahashi, Tomoya, Ito, Kunitoshi, Abe, Jumpei, Suzuki, Tomohiro, Wakana, Shigeharu, Fukamizu, Akiyoshi, Nakamura, Nobuhiro, Hirose, Shigehisa

    Plos One   8 ( 7 )   2013

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    DOI: 10.1371/journal.pone.0069451

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  • MARCH7 E3 ubiquitin ligase is highly expressed in developing spermatids of rats and its possible involvement in head and tail formation Reviewed

    Zhao, Boqiang, Ito, Kunitoshi, Iyengar, Prasanna Vasudevan, Hirose, Shigehisa, Nakamura, Nobuhiro

    Histochemistry and Cell Biology   139 ( 3 )   447 - 460   2013

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    DOI: 10.1007/s00418-012-1043-z

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  • Ubiquitination regulates the morphogenesis and function of sperm organelles. Reviewed

    Nakamura, Nobuhiro

    Cells   2 ( 4 )   732 - 50   2013

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    DOI: 10.3390/cells2040732

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  • Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids Reviewed

    Yogo, Keiichiro, Tojima, Hidehiro, Ohno, Jun-ya, Ogawa, Takuya, Nakamura, Nobuhiro, Hirose, Shigehisa, Takeya, Tatsuo, Kohsaka, Tetsuya

    Histochemistry and Cell Biology   137 ( 1 )   53 - 65   2012

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    DOI: 10.1007/s00418-011-0887-y

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  • MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound beta(2)-adrenergic receptors Reviewed

    Han, Sang-oh, Xiao, Kunhong, Kim, Jihee, Wu, Jiao-Hui, Wisler, James W., Nakamura, Nobuhiro, Freedman, Neil J., Shenoy, Sudha K.

    Journal of Cell Biology   199 ( 5 )   817 - 830   2012

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    DOI: 10.1083/jcb.201208192

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  • Membrane-associated RING-CH 10 (MARCH10 Protein) Is a Microtubule-associated E3 Ubiquitin Ligase of the Spermatid Flagella Reviewed

    Iyengar, Prasanna Vasudevan, Hirota, Tsuyoshi, Hirose, Shigehisa, Nakamura, Nobuhiro

    Journal of Biological Chemistry   286 ( 45 )   39082 - 39090   2011

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    DOI: 10.1074/jbc.M111.256875

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  • The Role of the Transmembrane RING Finger Proteins in Cellular and Organelle Function. Reviewed

    Nakamura, Nobuhiro

    Membranes   1 ( 4 )   354 - 393   2011

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    DOI: 10.3390/membranes1040354

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  • Rhesus Glycoprotein P2 (Rhp2) Is a Novel Member of the Rh Family of Ammonia Transporters Highly Expressed in Shark Kidney Reviewed

    Nakada, Tsutomu, Westhoff, Connie M., Yamaguchi, Yoko, Hyodo, Susumu, Li, Xiaojin, Muro, Takayuki, Kato, Akira, Nakamura, Nobuhiro, Hirose, Shigehisa

    Journal of Biological Chemistry   285 ( 4 )   2653 - 2664   2010

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    DOI: 10.1074/jbc.M109.052068

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  • Identification of zebrafish Fxyd11a protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis Reviewed

    Saito, Kaori, Nakamura, Nobuhiro, Ito, Yusuke, Hoshijima, Kazuyuki, Esaki, Masahiro, Zhao, Boqiang, Hirose, Shigehisa

    Frontiers in Physiology   1   2010

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    DOI: 10.3389/fphys.2010.00129

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  • A Novel Potential Role for Gametogenetin-Binding Protein 1 (GGNBP1) in Mitochondrial Morphogenesis During Spermatogenesis in Mice Reviewed

    Aihara, Takeshi, Nakamura, Nobuhiro, Honda, Shinji, Hirose, Shigehisa

    Biology of Reproduction   80 ( 4 )   762 - 770   2009

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    DOI: 10.1095/biolreprod.108.074013

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  • Mechanism of development of ionocytes rich in vacuolar-type H+-ATPase in the skin of zebrafish larvae Reviewed

    Esaki, Masahiro, Hoshijima, Kazuyuki, Nakamura, Nobuhiro, Munakata, Keijiro, Tanaka, Mikiko, Ookata, Kayoko, Asakawa, Kazuhide, Kawakami, Koichi, Wang, Weiyi, Weinberg, Eric S., Hirose, Shigehisa

    Developmental Biology   329 ( 1 )   116 - 129   2009

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    DOI: 10.1016/j.ydbio.2009.02.026

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  • Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane Reviewed

    Nakamura, Nobuhiro, Hirose, Shigehisa

    Molecular Biology of the Cell   19 ( 5 )   1903 - 1911   2008

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    DOI: 10.1091/mbc.E07-11-1103

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  • MARCH-XI, a novel transmembrane ubiquitin ligase implicated in ubiquitin-dependent protein sorting in developing spermatids Reviewed

    Morokuma, Yuri, Nakamura, Nobuhiro, Kato, Akira, Notoya, Michitaka, Yamamoto, Yoko, Sakai, Yasuhiro, Fukuda, Hidekazu, Yamashina, Shohei, Hirata, Yukio, Hirose, Shigehisa

    Journal of Biological Chemistry   282 ( 34 )   24806 - 24815   2007

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    DOI: 10.1074/jbc.M700414200

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  • MARCH-V is a novel mitofusin 2-and Drp1-binding protein able to change mitochondrial morphology Reviewed

    Nakamura, Nobuhiro, Kimura, Yasuo, Tokuda, Masaki, Honda, Shinji, Hirose, Shigehisa

    Embo Reports   7 ( 10 )   1019 - 1022   2006

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    DOI: 10.1038/sj.embor.7400790

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  • MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II Reviewed

    Fukuda, H, Nakamura, N, Hirose, S

    Journal of Biochemistry   139 ( 1 )   137 - 145   2006

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    DOI: 10.1093/jb/mvj012

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  • Role of C-terminus of Kir7.1 potassium channel in cell-surface expression Reviewed

    Tateno, T, Nakamura, N, Hirata, Y, Hirose, S

    Cell Biology International   30 ( 3 )   270 - 277   2006

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    DOI: 10.1016/j.cellbi.2005.11.007

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  • MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking Reviewed

    Nakamura, N, Fukuda, H, Kato, A, Hirose, S

    Molecular Biology of the Cell   16 ( 4 )   1696 - 1710   2005

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    DOI: 10.1091/mbc.E04-03-0216

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  • 腎K+分泌能の発達における内向き整流性K+チャネル(ROMK,Kir7.1)の発現

    鈴木 喜郎, 安岡 有紀子, 中村 信大, 広瀬 茂久, 河原 克雅

    発達腎研究会誌   12 ( 1 )   13 - 15   2004.9

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    腎K+分泌能の発達における内向き整流性とK+チャンネル(ROMK,Kir7.1)の発現について検討した.方法は,生後7,14,21日の新生仔ラットにKCI溶液を飲ませ,K+負荷後0,2,4,6時間後に採尿した.尿中K+排泄量は生後7日に比し生後14日で約2倍に増加したが,生後21日では差はなかった.1/2K+排泄に要する時間は日齢により減少し続けた.生後7,14,21日の新生仔ラット腎臓におけるROMK1 mRNAは生後7日で既に発現しており,7日から14日の間に約2倍に増加したが,14日から21日の間ではあまり変化はなかった.Kir7.1 mRNAは生後14日から21日の間に発現量が有意に増加した.免疫組織染色では,Kir7.1は生後7日にはヘンレの太い上行脚(TAL)の側底膜に存在しており,日齢を経て遠位曲尿細管及び皮質集合管の側底膜のシグナルが増加した.Ab-ROMK抗体は全ての日齢でTALの管腔膜にシグナルを認めた

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  • Expression of the K+ channel Kir7.1 in the developing rat kidney: Role in K+ excretion Reviewed

    Suzuki, Y, Yasuoka, Y, Shimohama, T, Nishikitani, M, Nakamura, N, Hirose, S, Kawahara, K

    Kidney International   63 ( 3 )   969 - 975   2003

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    DOI: 10.1046/j.1523-1755.2003.00806.x

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  • RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica Reviewed

    Miyamoto, K, Nakamura, N, Kashiwagi, M, Honda, S, Kato, A, Hasegawa, S, Takei, Y, Hirose, S

    European Journal of Biochemistry   269 ( 24 )   6152 - 6161   2002

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    DOI: 10.1046/j.1432-1033.2002.03332.x

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  • Complex structure and regulation of expression of the rat gene for inward rectifier potassium channel Kir7.1 Reviewed

    Nakamura, N, Suzuki, Y, Ikeda, Y, Notoya, M, Hirose, S

    Journal of Biological Chemistry   275 ( 36 )   28276 - 28284   2000

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    DOI: 10.1074/jbc.M003734200

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  • Localization of inward rectifier potassium channel Kir7.1 in the basolateral membrane of distal nephron and collecting duct Reviewed

    Ookata, K, Tojo, A, Suzuki, Y, Nakamura, N, Kimura, K, Wilcox, CS, Hirose, S

    Journal of the American Society of Nephrology   11 ( 11 )   1987 - 1994   2000

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  • Inward rectifier potassium channel Kir7.1 is highly expressed in the ion-transporting epithelial cells:: implication for a functional coupling with Na+,K+-ATPase

    Nobuhiro Nakamura

    CONTROL AND DISEASES OF SODIUM DEPENDENT TRANSPORT PROTEINS AND ION CHANNELS   1208   2000

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  • Na,K-ATPaseとの機能共役が期待されるKチャネル(Kir7.1)の細胞局在とTSH応答

    中村 信大, 鈴木 喜郎, 池田 雄吾, 大方 香代子, 河原 克雅, 広瀬 茂久

    生化学   71 ( 8 )   807 - 807   1999.8

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  • 新しいカリウムチャネルKir7.1の遠位側ネフロンの基底膜における局在

    大方 香代子, 藤乗 嗣泰, 中村 信大, 鈴木 喜郎, 木村 健二郎, 後藤 淳郎, 藤田 敏郎, 広瀬 茂久

    日本腎臓学会誌   41 ( 3 )   275 - 275   1999.5

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  • Inwardly rectifying K+ channel Kir7.1 is highly expressed in thyroid follicular cells, intestinal epithelial cells and choroid plexus epithelial cells:: implication for a functional coupling with Na+,K+-ATPase Reviewed

    Nobuhiro Nakamura

    BIOCHEMICAL JOURNAL   342 ( 2 )   329 - 336   1999

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    DOI: 10.1042/0264-6021:3420329

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  • Identification by differential display of a hypertonicity-inducible inward rectifier potassium channel highly expressed in chloride cells Reviewed

    Suzuki, Y, Itakura, M, Kashiwagi, M, Nakamura, N, Matsuki, T, Sakuta, H, Naito, N, Takano, K, Fujita, T, Hirose, S

    Journal of Biological Chemistry   274 ( 16 )   11376 - 11382   1999

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    DOI: 10.1074/jbc.274.16.11376

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MISC

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Presentations

  • 免疫制御を担う新規受容体分子の肺血管内皮細胞のニコチン応答と炎症応答に及ぼす影響

    中村信大, 山田翔太

    喫煙科学研究財団第37回令和4年度研究助成発表会  2023.7 

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    Event date: 2023.7

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • タバコ煙抽出液処理によるマウス肺血管内皮細胞におけるGタンパク質共役型受容体Adgrf5の発現変動

    山田 翔太, 中村 信大

    第45回日本分子生物学会年会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Poster presentation  

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  • 細胞外マトリックス異常を伴うノックアウトマウスを用いたGPCR分子の新機能解明を目指した研究 Invited

    中村信大, 山田翔太

    第一回LiHubバイオマトリックスミニシンポジウム  2022.11 

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    Event date: 2022.11

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  • Targeted disruption of GPR116 (ADGRF5) impairs the integrity of the glomerular filtration barrier in mice Invited

    Nobuhiro Nakamura

    TOIN International Symposium on Biomedical Engineering 2022  2022.11 

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    Event date: 2022.11

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  • 免疫制御を担う新規受容体分子の肺血管内皮細胞のニコチン応答と炎症応答に及ぼす影響

    中村 信大, 山田 翔太

    喫煙科学研究財団第36回令和3年度研究助成発表会  2022.7 

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    Event date: 2022.7

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  • Ubiquitination of syntaxin-6 by the E3 ubiquitin ligase MARCH2

    2020.9 

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    Event date: 2020.9

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  • Increased expression of polyamine biosynthetic and catabolic enzymes in alveolar macrophages of Ig-Hepta knockout mice

    Hikaru ANDO, Donna Maretta Ariestanti, Shigehisa HIROSE, Nobuhiro NAKAMURA

    The 9th TOIN International Symposium on Biomedical Engineering 2014  2014.11 

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    Event date: 2014.11

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  • Early cardiac connexin acts as a Ca2+ hemichannel that regulates heart morphogenesis via NFATc4 activation

    Naznin Sultana, Kakon Nag, Akira Kato, Atsushi Kawakami, Koichi Kutsuzawa, Toshihiro Akaike, Shigehisa Hirose, Nobuhiro Nakamura

    The ISSCR 12th Annual Meeting  2014.6 

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    Event date: 2014.6

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  • Regulation of mitochondrial morphology by ubiquitination

    Nobuhiro Nakamura

    International Marine Biotechnology Symposium  2013.5 

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    Event date: 2013.5

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

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  • USP19 deubiquitinating enzyme regulates stability of TEB4 ubiquitin ligase

    Nobuhiro NAKAMURA, Yuki EBISAWA, Shigehisa HIROSE

    Joint Meeting of JSDB 45th and JSCB 64th  2012.5 

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    Event date: 2012.5

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  • Regulation of mitochondrial morphology by ubiquitination Invited

    Nobuhiro Nakamura

    5th Conference of Korean Society for Mitochondrial Research and Medicine  2011.6 

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    Event date: 2011.6

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  • ミトコンドリア形態制御因子に相互作用する新規ユビキチン化酵素 MARCH-V の同定 Invited

    中村信大

    第30回日本分子生物学会年会・第80回日本生化学会大会合同大会  2007.12 

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    Event date: 2007.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • Cloning and properties of a hypertonicity-inducible inward rectifier K+ channel from euryhaline eels

    Proceedings of the 23rd Taniguchi Foundation Biophysics Symposium  1998 

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  • Cloning and properties of a hypertonicity-inducible inward rectifier K+ channel from euryhaline eels

    Proceedings of the 23rd Taniguchi Foundation Biophysics Symposium  1998 

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  • Kdg1, a novel RING-finger membrane protein that localizes in the recycling endosome compartment.

    42nd american society for cell biology annual meeting  2002 

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  • Kdg1, a novel RING-finger membrane protein that localizes in the recycling endosome compartment.

    42nd american society for cell biology annual meeting  2002 

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  • Intracellular signaling Mechanism of G-protein coupled receptor ADGRF5

    Tomoya Sato, Masako Kato, Humimasa Kubo, Nobuhiro Nakamura

    13th Toin International Symposium on Biomedical Engineering  2018.10 

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  • Regulatory mechanism of protein expression of human G-protein coupled recept

    2020.9 

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  • Deletion of Adgrf5 causes abnormal glomerular filtration barrier in mice

    2020.9 

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  • A549細胞におけるUSP30-AS1の発現制御とUSP30の発現への影響の解析

    松本 聖慈, 加藤 真子, 中村 信大

    第94回日本生化学大会  2021.11 

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  • The expression of zebrafish cx36.7 gene is regulated by GATA4 in early heart development

    Hisako Miyagi, Ippei Kasajima, Yumiko Tanaka, Keijiro Munakata, Kakon Nag, Naznin Sultana, Shigehisa Hirose, Nobuhiro Nakamura

    2014.5 

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  • Emphysema-like symptoms in lungs of Ig-Hepta/GPR116-deficient mice

    Donna Maretta Ariestanti, Akira Kato, Tomoya Takahashi, Shigehisa Hirose, Nobuhiro Nakamura

    7th international adhesion GPCR workshop  2014.6 

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  • Emphysema-like symptom in Ig-Hepta/Gpr116 knockout mice associated to alveolar macrophage activation

    Donna Maretta Ariestanti, Hikaru Ando, Shigehisa Hirose, Nobuhiro Nobuhiro Nakamura

    10th Toin International Symposium on Biomedical Engineering  2015.11 

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  • Regulation of cardiac-specific expression of the zebrafish cx36.7 gene

    Hisako Miyagi, Kakon Nag, Naznin Sultana, Keijiro Munakata, Shigehisa Hirose, Nobuhiro Nakamura

    Toin International Symposium on Biomedical Engineering  2015.11 

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  • A novel mechanism for the regulation of the expression of the mitochondrial deubiquitinating enzyme USP30

    Mizuho Muto, Masako Kato, Nobuhiro Nakamura

    11th Toin International Symposium on Biomedical Engineering  2016.10 

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  • Adgrf5 deficient mice exhibit bronchial asthma- and fibrosis-like phenotypes

    Fumimasa Kubo, Donna Maretta Ariestanti, Souta Oki, Rahmaningsih Mara Sabirin, Ryotarou Demizu, Nobuhiro Nakamura

    12th Toin International Symposium on Biomedical Engineering  2017.11 

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  • Adgrf5欠損マウスは気管支喘息および肺線維症様の表現型を現す

    久保 文雅, Donna Ariestanti, 沖 颯太, Rahmaningsih Sabirin, 出水 遼太郎, 中村 信大

    2017年度生命科学系学会合同年次大会  2017.12 

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  • Gタンパク質共役型受容体ADGRF5の欠損は肺血管内皮細胞における炎症性分子の発現と喘息に似た表現型を誘導する

    久保 文雅, Donna Ariestanti, 沖 颯太, 福澤拓, 出水 遼太郎, 佐藤朋哉, Rahmaningsih Sabirin, 広瀬茂久, 中村 信大

    第91回日本生化学会大会  2018.9 

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  • Kdg2, a novel multidomain protein involved in the regulation of recycling endosomal trafficking.

    HUPO 2nd & IUBMB XIX World Congress  2003 

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  • Kdg2, a novel multidomain protein involved in the regulation of recycling endosomal trafficking.

    HUPO 2nd & IUBMB XIX World Congress  2003 

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  • Cardiac expression of zebrafish connexin 36.7 is regulated by GATA4 transcription factor

    Hisako Miyagi, Ippei Kasajima, Yumiko Tanaka, Keijiro Munakata, Kakon Nag, Naznin Sultana, Shigehisa Hirose, Nobuhiro Nakamura

    8th International Symposium on Biomedical Engineering  2013.10 

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  • The deubiquitinating enzyme USP19 regulates the ERAD pathway by stabilizing the E3 ubiquitin ligases

    Kumi Harada, Shigehisa Hirose, Nobuhiro Nakamura

    8th International Symposium on Biomedical Engineering  2013.10 

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  • Regulation of the activity of USP19 deubiquitinating enzyme.

    Yuki Ebisawa, Nobuhiro Nakamura

    8th International Symposium on Biomedical Engineering  2013.10 

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  • Control of mitochondrial morphology by ubiquitination and deubiquitination Invited

    Nobuhiro NAKAMURA

    8th International Symposium on Biomedical Engineering  2013.10 

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  • 糸球体濾過障壁の恒常性維持におけるGPCRの新機能 第54回関東腎研究会 Invited

    中村信大

    第54回関東腎研究会  2026.1 

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  • ADGRF5 mediates shear stress mechanotransduction in glomerular endothelial cells

    Xiaoyang Wang, Masako Kato, Miki Nagase, Nobuhiro Nakamura

    2026.6 

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  • Glomerular endothelial cell receptor adhesion G-protein–coupled receptor F5 (ADGRF5) and the integrity of the glomerular filtration barrier. Invited

    Nakamura N

    Kidney Week 2024, The Best of ASN Journals: JASN, CJASN, and Kidney360  2024.10 

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  • 免疫制御を担う新規受容体分子の肺血管内皮細胞のニコチン応答と炎症応答に及ぼす影響

    中村 信大, 山田翔太

    喫煙科学研究財団第38回令和5年度研究助成発表会  2024.7 

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  • Characterization of a novel oxidative stress-responsive ubiquitin ligase RNF183

    Kyohei Ogihara, Yohei Hashimoto, Masako Kato, Shota Yamada, Nobuhiro Nakamura

    20th TOIN International Symposium on Biomedical Engineering  2025.10 

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  • G-protein-coupled receptor ADGRF5 enhances shear stress-induced intracellular signaling.

    Xiaoyang Wang, Masako Kato, Kazuki Yamamoto, Nobuhiro Nakamura

    19th TOIN International Symposium on Biomedical Engineering  2024.10 

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  • Effects of cigarette smoke extracts on the expression of the G protein-coupled receptor Adgrf5 and KLF2 in mouse pulmonary endothelial cells

    Yamada S., Nakamura N.

    18th TOIN International Symposium on Biomedical Engineering 2023  2023.12 

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Awards

  • Best of ASN Journals 2024

    2024.10   The American Society of Nephrology   Glomerular endothelial cell receptor adhesion G-protein–coupled receptor F5 (ADGRF5) and the integrity of the glomerular filtration barrier

    Nagase M., Ando H., Beppu Y., Kurihara H., Oki S., Kubo F., Yamamoto K., Nagase T., Kaname S., Akimoto Y., Fukuhara H., Sakai T., Hirose S., Nakamura N.

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Research Projects

  • 糸球体の血液濾過フィルターの恒常性維持機構:受容体分子ADGRF5と力学刺激の役割

    Grant number:24K09452  2024.4 - 2027.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    中村 信大

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    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

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  • 新規ホルモンFNDC4とその受容体ADGRF5の血管内皮細胞の機能調節に及ぼす影響に関する研究

    2023.4 - 2024.3

    小柳財団  小柳財団・2023年度研究助成金 

    中村 信大

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  • 免疫制御を担う新規受容体分子の肺血管内皮細胞のニコチン応答と炎症応答に及ぼす影響

    2021.4 - 2024.3

    喫煙科学研究財団  喫煙科学研究財団研究助成(一般研究) 

    中村信大

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  • オーファン受容体Adgrf5による糸球体濾過障壁の恒常性維持機構の解明

    Grant number:21K06167  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    中村 信大

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    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    ①糸球体血管内皮細胞におけるADGRF5の細胞内シグナル伝達機構について明らかにするため、正常およびADGRF5ノックアウトマウス(以後、KOマウスと呼ぶ)から単離した糸球体および糸球体血管内皮細胞におけるKLF2、KLF4、eNOSの遺伝子発現量について定量PCR解析を行った。その結果、いずれの場合においても遺伝子発現量がKOマウスで上昇することを認めた。タンパク質レベルでもeNOSの発現量増加は確認できたが、eNOSのリン酸化レベルは変化が認められなかった。このことから、KOマウスではKLF2/4を発現誘導するシグナル経路が活性化していることが示唆された。またKOマウスではeNOSのリン酸化レベルが低下し、それを補うためにKLF2/4を介してeNOSの発現を上昇している可能性も考えられる。
    ②KOマウスの糸球体濾過機能異常のメカニズムを解明するために、血管内皮細胞表面に局在しタンパク質透過性を制御する分子の遺伝子発現について解析した。その結果、KOマウスにおけるグリコカリックス成分の遺伝子の発現量が顕著に減少していることを確認した。グリコカリックスの異常はタンパク尿を引き起こすことが報告されていることから、グリコカリックス成分の発現低下がのKOマウスにおけるタンパク尿の発症に寄与する可能性が示唆された。
    ③ADGRF5のリガンドとして新規ホルモン分子であるFNDC4を同定した。FNDC4は肝臓から分泌され、脂肪細胞のADGRF5に作用することでインスリン感受性を上昇させる効果があることを明らかにした。また、脂肪細胞においてFNDC4はADGRF5依存的に細胞内cAMP濃度の上昇を引き起こすことを認めた。

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  • Role of Gpr116 in maintenance of the glomerular filtration barrier in mice

    Grant number:16K07344  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Nakamura Nobuhiro

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    In this study, 1) we showed that Gpr116 is localized to the glomerular endothelial cells by immunoelectron microscopy. 2) We identified genes of which expression are altered in the glomerular endothelial cells of Gpr116-deficient mice. 3) We identified candidates of downstream signaling molecules, which are activated by Gpr116 overexpression. 4) We observed that several intracellular signaling pathways were activated to a lesser extent in Gpr116 overexpressing cells by shear stress.

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  • ゼブラフィッシュの心筋細胞分化の鍵を担う新規コネキシン分子の機能解析

    2013.4 - 2014.3

    武田科学振興財団  武田科学振興財団ライフサイエンス研究奨励 

    中村信大

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  • Roles of the organelle-specific ubiquitination regulators in protein degradation

    Grant number:24570209  2012.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    NAKAMURA Nobuhiro

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    Grant amount:\5070000 ( Direct Cost: \3900000 、 Indirect Cost:\1170000 )

    Although protein quality control is essential for maintaining cellular function, its molecular mechanism is not fully understood. In this study, we performed the characterization of the endoplasmic-reticulum (ER) deubiquitinating enzyme USP19 and showed that USP19 stabilizes the ER-associated ubiquitinating enzyme MARCH6 via deubiquitination. It is known that MARCH6 has a role in protein quality control in the ER. In addition, the stabilization of MARCH6 increases its enzymatic activity and thereby promotes degradation of the mutant ABCB11, a substrate of MARCH6. These results suggest that USP19 is involved in the regulation of protein quality control in the ER.

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  • 細胞内Caシグナルのスイッチとなる新発見のコネキシンの生理機能と発現制御機構

    Grant number:12F02392  2012.4 - 2015.3

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    中村 信大, SULTANA Naznin, SULTANA N.

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    私たちは、ゼブラフィッシュおいてコネキシン36.7(Cx36.7)の機能不全が心筋の筋原繊維の配向の乱れを引き起こし、心臓形成と機能不全に至る事を明らかにしてきた。本研究では、コネキシン36.7(Cx36.7)の心筋前駆細胞特異的な発現の調節機構について明らかにする解析を行った。心筋細胞の分化誘導に関与する転写因子の発現パターンについて,心臓形成時のゼブラフィッシュ稚魚を用いてin situ hybridization法により調べ,Cx36.7の発現パターンを比較したところ,発現のタイミングと局在いずれにおいてGata4がCx36.7と一致することが分かった。そこで,Gata4の発現をアンチセンスモルフォリノにより特異的に抑制したところ,Cx36.7遺伝子のプロモーター活性が著しく下がることが分かった。同様な結果は,プロモーター内部のGata型転写因子の結合配列に変異を導入しても認められた。この結合配列がGata4との相互作用部位であることをin vitroのゲルシフト解析で確認した。以上の結果から,Cx36.7遺伝子の心筋細胞特異的な発現はGata4による転写制御によるところが大きいことが分かった。また、Cx36.7の下流のシグナル伝達について解析を行い、候補転写因子を一つ同定することが出きた。現在、この分子の機能解析を行っている。

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  • コネキシンを介したカルシウムシグナリングによる筋原繊維形成の分子機構の解析

    2012.4 - 2013.3

    倉田記念日立科学技術財団  倉田奨励金 

    中村 信大

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  • Cellular role and regulatory mechanism for a novel deubiquitinating enzyme in the endoplasmic reticulum

    Grant number:22770123  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    NAKAMURA Nobuhiro

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    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

    This study has demonstrated that 1) USP19, a transmembrane deubiquitinating enzyme in the endoplasmic reticulum(ER), deubiquitinates not only typical substrates of ER-associated degradation(ERAD), but also ERAD ubiquitinating enzymes, and 2) the enzymatic activity of USP19 is dependent on the domain structures and processing of USP19. These results provide better understand of the molecular mechanism for the regulation of the ERAD pathway as well as the USP19 activity.

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  • Functional characterization of novel proteins involved in mitochondrial dynamics

    Grant number:20770156  2008 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    NAKAMURA Nobuhiro

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    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

    This study has demonstrated that 1) an enzymatic activity of human USP30, a novel mitochondrial deubiquitinating enzyme, is required for maintenance of mitochondrial morphology, and 2) mouse GGNBP1 is highly expressed in spermatogenic cells and induces Drp1-dependent mitochondrial fragmentation. These results provide better understand of the molecular mechanism of mitochondrial dynamics.

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  • 細胞内輸送システムに関与する新しい分子の細胞生物学的研究

    2004 - 2005

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  • Cell biological study for novel molecules involved in intracellular trafficking

    2004 - 2005

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