Updated on 2026/07/14

写真a

 
PENG ZUGUI
 
Organization
School of Engineering Assistant Professor
Title
Assistant Professor
External link

Degree

  • PhD in Engineering ( 2022.10   Tokyo Institute of Technology )

  • M.S ( 2019.9   Tokyo Institute of Technology )

  • B.C. ( 2018.3   Tokyo Institute of Technology )

Research Areas

  • Nanotechnology/Materials / Nanobioscience

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

  • Natural Science / Biophysics, chemical physics and soft matter physics

Research History

  • Institute of Science Tokyo   Assistant Professor

    2026.4

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  • Tokyo University of Agriculture and Technology   Faculty of Engineering Department of Biotechnology and Life Science   Specially Appointed Assistant Professor

    2022.9 - 2026.3

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  • Tokyo Institute of Technology

    2021.4 - 2022.9

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Papers

  • Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing. Reviewed International journal

    Zugui Peng, Daisuke Noshiro, Shiroh Futaki, Ryuji Kawano

    Angewandte Chemie (International ed. in English)   e3311099   2026.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Creating alternative nanopores is crucial for advancing single-molecule methodologies. Peptide nanopores scaffolded by DNA nanostructures have emerged as a promising class of chemically synthesizable nanopores with organized geometries. However, no such nanopores have yet achieved single-molecule detection due to size limitations. Here, we overcome this limitation by employing the alamethicin (ALM) peptide, based on the hypothesis that peptides with looser helix-helix packing are advantageous for constructing large-diameter nanopores. The resulting DNA-ALM nanopores exhibited long-lived open states and conductance values comparable to those of natural protein nanopores. To further enhance pore functionality, we introduced asymmetric electrostatic forces by harnessing the intrinsic helix dipole of ALM, which facilitated pore expansion and improved ion transport efficiency. This modification led to enhanced conductance levels and enabled single-molecule sensing of biomolecules, including DNA and peptides. Overall, this work demonstrates a generalizable strategy for constructing functional, chemically synthesizable nanopores, providing a foundation for programmable nanopore design and next-generation biosensing applications.

    DOI： 10.1002/anie.3311099

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  • Construction and characterization of a nanopore derived from the transmembrane domain of a trimeric autotransporter adhesin. Reviewed International journal

    Jun Sasahara, Shogo Yoshimoto, Zugui Peng, Taehyun Hwang, Iori Kobayashi, Ryuji Kawano, Katsutoshi Hori

    Frontiers in bioengineering and biotechnology   14   1764864 - 1764864   2026

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    Language：English   Publishing type：Research paper (scientific journal)  

    Bacterial secretion systems (SSs) are increasingly recognized as biological nanopores with potential biotechnological applications. Here, we engineered the transmembrane β-barrel of a trimeric autotransporter adhesin (TAA) secreted by the type Vc SS. The coiled-coil segment that occupies the central lumen of the transmembrane β-barrel of an Acinetobacter TAA, AtaA, was removed to design an open β-barrel pore, termed AtaApore. Polypeptides of AtaApore were produced using a cell-free expression system and reconstituted into lipid membranes. Electrophysiological measurements showed ion channel activity of AtaApore with a median conductance of 0.17 nS. Molecular dynamics simulations revealed ion transport properties, including transient trapping of Cl- ions at a constriction formed by R3597 and R3622. Together, to our knowledge, these results provide the first characterization of a nanopore derived from a TAA secreted by the type Vc SS. AtaApore provides a new scaffold for nanopore engineering and a simplified model for probing the mechanism of the type Vc SS.

    DOI： 10.3389/fbioe.2026.1764864

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  • De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs. Reviewed International journal

    Zugui Peng, Masataka Usami, Ayaka Nakada, Yusuke Sekiya, Shoko Fujita, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano

    ACS nano   2025.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Nanopore sensing using transmembrane proteins holds great potential for single-molecule detection. The creation of pore-forming proteins is an emerging research direction, and one promising strategy is the bottom-up de novo design of nanopores. However, de novo α-helical nanopores with sufficiently large dimensions to accommodate single-molecule sensing have not been previously reported. Here, we designed and constructed α-helical nanopores based on two helix-packing motifs, GX6G and GX3G, which we named FFK and LEK. Electrical recordings revealed that both FFK and LEK form nanopores in lipid membranes with high conductance. FFK exhibited multiple conductance states, whereas LEK formed more monodisperse pores. Although both nanopores assembled via a stepwise monomer-joining mechanism, the assembly and disassembly dynamics of FFK were significantly faster than those of LEK. We further demonstrated the single-molecule sensing capabilities of both pores, showing that they can detect cyclodextrin derivatives, while LEK could additionally sense poly-l-lysine. Overall, these results demonstrate the feasibility of designing functional α-helical nanopores for nanopore sensing technologies.

    DOI： 10.1021/acsnano.5c15080

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  • Unraveling the time course of interaction between DNA nanopores and lipid bilayers using QCM-D: role of cholesterol anchors and bilayer supporting substrates. Reviewed International journal

    Zugui Peng, Glenn Villena Latag, Hiroyuki Tahara, Tohru Yagi, Tomohiro Hayashi

    Nanoscale   2025.4

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Lipid membranes are fundamental elements of cells, serving as barriers that protect the cell interior from the external environment. DNA nanostructures, which can engineer lipid membranes' signal transduction and substance exchange properties, attract interest for their potential applications in the biomedical field. The interaction between DNA nanostructures and lipid membranes is of scientific and technological interest. Here, we investigate the interaction between DNA nanopores (DNPs) and model supported lipid bilayers (SLBs) using real-time quartz crystal microbalance with energy dissipation monitoring (QCM-D). The long observation time and high temporal resolution enable us to visualize the time course of DNPs' interaction with SLBs, including their tethering and incorporating processes. Benefiting from the ability of QCM-D to obtain adsorbed layers' viscoelasticity profiles, we found that the DNPs with three cholesterol tags aggregate and form a rigid layer on the SLB surface during their tethering step. Moreover, our results reveal that the supporting substrates of SLBs impact the incorporation of DNPs, whereby separating the SLBs from the SiO2 sensor surface with poly(ethylene glycol) (PEG) polymer cushion results in faster incorporation. This study not only sheds light on the behavior of DNPs but also establishes QCM-D as an analytical platform for exploring the interactions of membrane-interacting DNA nanostructures, potentially accelerating advancements in DNA nanotechnology.

    DOI： 10.1039/d5nr01299f

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  • Synthetic DNA nanopores for direct molecular transmission between lipid vesicles. Reviewed International journal

    Zugui Peng, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    Nanoscale   16 ( 25 )   12174 - 12183   2024.6

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Lipid vesicles hold potential as artificial cells in bottom-up synthetic biology, and as tools in drug delivery and biosensing. Transmitting molecular signals is a key function for vesicle-based systems. One strategy to achieve this function is by releasing molecular signals from vesicles through nanopores. Nevertheless, in this strategy, an excess of molecular signals may be required to reach the targets, due to the dispersion of the signals during diffusion. The key to achieving the efficient utilization of signals is to shorten the distance between the sender vesicle and the target. Here, we present a pair of DNA nanopores that can connect and form a direct molecular pathway between vesicles. The nanopores are self-assembled from nine single DNA strands, including six 14-nucleotide single-stranded overhangs as sticky-end segments, enabling them to bind with each other. Incorporating nanopores shortens the distance between different populations of vesicles, allowing less diffusion of molecules into bulk solution. To further reduce the loss of molecules, a DNA nanocap is added to one of the nanopore's openings. The nanocap can be removed through the toehold-mediated DNA strand displacement when the nanopore meets its counterpart. Our DNA nanopores provide a novel molecular transmission tool to lipid vesicles-based systems.

    DOI： 10.1039/d4nr01344a

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  • Effects of inserting ultrashort carbon nanotubes into lipid bilayers on membrane morphology Reviewed

    Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    Electronics and Communications in Japan   144 ( 5 )   424 - 430   2024.6

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    Language：Japanese   Publisher：Wiley  

    Abstract

    Single‐walled carbon nanotubes (CNTs) are carbon materials with unique thermal, optical, mechanical, and electrical properties, with hollow cylindrical structures of a few nanometers in diameter. CNTs cut to about 10 nm (Ultrashort CNTs, US‐CNTs) can spontaneously insert into lipid bilayers. Therefore, applications have been proposed to combine CNTs with lipid bilayers to give the membranes the properties of CNTs. However, CNTs interact with membranes to induce morphological changes in the membranes, which may hinder these applications. In this study, to investigate the effects, US‐CNTs are exposed to lipid bilayer vesicles (giant unilamellar vesicles, GUVs), which are used as a model for cell membranes, and the changes in membrane morphology with each US‐CNT concentration were evaluated by fluorescence microscopy. As a result, GUVs show morphological changes upon exposure to US‐CNTs, eventually transforming into a multiple vesicle‐linked shape. This result suggests an increase in the area and asymmetry of the GUV membrane. Based on these results, we have proposed a hypothesis regarding the mechanism of morphological changes induced in the GUV membranes by US‐CNTs exposure.

    DOI： 10.1002/ecj.12461

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  • Ultrasound-Responsive Liposome-Encapsulated Gel Patches Drug Delivery Model Reviewed

    Kano Kajie, Shoichiro Kanno, Zugui Peng, Kenta Shimba, Takashi Shibata, Yoshitaka Miyamoto, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   144 ( 5 )   465 - 466   2024.5

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    Publishing type：Research paper (scientific journal)   Publisher：Institute of Electrical Engineers of Japan (IEE Japan)  

    DOI： 10.1541/ieejeiss.144.465

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  • Lipid vesicle-based molecular robots. Reviewed International journal

    Zugui Peng, Shoji Iwabuchi, Kayano Izumi, Sotaro Takiguchi, Misa Yamaji, Shoko Fujita, Harune Suzuki, Fumika Kambara, Genki Fukasawa, Aileen Cooney, Lorenzo Di Michele, Yuval Elani, Tomoaki Matsuura, Ryuji Kawano

    Lab on a chip   2024.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    A molecular robot, which is a system comprised of one or more molecular machines and computers, can execute sophisticated tasks in many fields that span from nanomedicine to green nanotechnology. The core parts of molecular robots are fairly consistent from system to system and always include (i) a body to encapsulate molecular machines, (ii) sensors to capture signals, (iii) computers to make decisions, and (iv) actuators to perform tasks. This review aims to provide an overview of approaches and considerations to develop molecular robots. We first introduce the basic technologies required for constructing the core parts of molecular robots, describe the recent progress towards achieving higher functionality, and subsequently discuss the current challenges and outlook. We also highlight the applications of molecular robots in sensing biomarkers, signal communications with living cells, and conversion of energy. Although molecular robots are still in their infancy, they will unquestionably initiate massive change in biomedical and environmental technology in the not too distant future.

    DOI： 10.1039/d3lc00860f

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  • A Study on Cell Culture Scaffolds using Magnetic Gel Reviewed

    Yuya Shimomura, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   143 ( 7 )   714 - 715   2023.7

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    Publishing type：Research paper (scientific journal)   Publisher：Institute of Electrical Engineers of Japan (IEE Japan)  

    DOI： 10.1541/ieejeiss.143.714

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  • A Study on DDS with High Response to Ultrasound using Metal Nanoparticle-encapsulated Liposomes Reviewed

    Yukine Tagai, Kano Kajie, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Takashi Shibata, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   143 ( 7 )   712 - 713   2023.7

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    Publishing type：Research paper (scientific journal)   Publisher：Institute of Electrical Engineers of Japan (IEE Japan)  

    DOI： 10.1541/ieejeiss.143.712

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  • Neural Interface for Biohybrid Prosthetic Hands to Realize Sensory and Motor Functions Reviewed

    Tohru Yagi, Zugui Peng, Shoichiro Kanno

    JOURNAL OF ROBOTICS AND MECHATRONICS   34 ( 2 )   316 - 318   2022.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.20965/jrm.2022.p0316

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  • Development of novel ECM which has stiffness difference based on hydrogel beads Reviewed

    Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    ELECTRONICS AND COMMUNICATIONS IN JAPAN   104 ( 3 )   2021.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/ecj.12321

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  • A Study of the Effects of Plasma Surface Treatment on Lipid Bilayers Self-Spreading on a Polydimethylsiloxane Substrate under Different Treatment Times Reviewed

    Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    LANGMUIR   37 ( 36 )   10732 - 10740   2021.9

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.langmuir.1c01319

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  • Effect of Magnetic Nanoparticle Internalization on Cell Density in Skeletal Muscle Tissue Reviewed

    Yuji Kirihara, Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Kazunori Shimizu, Hiroyuki Honda, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   141 ( 7 )   795 - 801   2021.7

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    Language：English   Publisher：Institute of Electrical Engineers of Japan (IEE Japan)  

    DOI： 10.1541/ieejeiss.141.795

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  • Formation of agarose-supported liposomes by polymer-assisted method toward biosensor Reviewed

    Zugui Peng, Kentaro Wada, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   141 ( 5 )   646 - 653   2021

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1541/ieejeiss.141.646

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  • Functional analysis of liposomes containing Single-Walled Carbon Nanotubes (SWNTs) by fluorescence microscopy Reviewed

    Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   141 ( 5 )   620 - 626   2021

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1541/ieejeiss.141.620

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  • Development of novel ECM which has stiffness difference based on hydrogel beads Reviewed

    Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    IEEJ Transactions on Electronics, Information and Systems   141 ( 5 )   607 - 613   2021

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  • Preparation of Size-controlled Giant Vesicles Under Physiological Conditions Reviewed

    Zugui Peng, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    42ND ANNUAL INTERNATIONAL CONFERENCES OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY: ENABLING INNOVATIVE TECHNOLOGIES FOR GLOBAL HEALTHCARE EMBC'20   2198 - 2201   2020

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    Authorship：Lead author   Language：English   Publishing type：Research paper (international conference proceedings)  

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  • Nanopore lipid bilayer formed by self-spreading method Reviewed

    Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    ELECTRONICS AND COMMUNICATIONS IN JAPAN   102 ( 12 )   47 - 54   2019.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/ecj.12225

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  • Ultrasound-triggered DNA-Based nanotubes: Electrophoretic evaluation on synthetic structures

    Shota Yoshizaki, Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    2024.11

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  • De novo design of nanopores for single-molecule detection that incorporates α-helix peptides

    Masataka Usami, Zugui Peng, Yusuke Sekiya, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano

    2024.2

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    <jats:p>Nanopore sensing using transmembrane proteins enjoys great potential for rapid and low-cost single-molecule detection. Creating pore-forming proteins or peptides is an emerging frontier in the field of nanopore sensing. One of the approaches for constructing new nanopores is bottom-up De novo protein design. Due to the difficulties in designing and expressing transmembrane proteins, peptides are the first design target, but so far, de novo α-helical nanopores with sizes large enough to transport biomolecules have not been reported. Here, we constructed α-helical nanopores based on GX6G and GX3G motifs, named FFK and LEK. FFK formed multidisperse pores with diameters of 0.9 to 1.4 nm while LEK formed monodisperse pores with a diameter of 0.9 nm. Though the larger diameter of FFK nanopores provides opportunities for molecular translocation, the too-fast join and dissociate of peptide monomers make them unsuited for nanopore sensing. On the other hand, translocation of poly-L-lysine through LEK nanopore could be observed by electrical recording, revealing their possibility for amino-acid sequencing. Our findings provide new opportunities to create designer nanopores for nanopore sensing technologies.</jats:p>

    DOI： 10.26434/chemrxiv-2024-wzql1

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  • Control of Drug Release in Ultrasound-Responsive Liposome-Encapsulated Gel Patches.

    Kano Kajie, Zugui Peng, Kenta Shimba, Takashi Shibata 0006, Yoshitaka Miyamoto, Tohru Yagi

    MeMeA   1 - 5   2023

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    Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/MeMeA57477.2023.10171928

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    Other Link： https://dblp.uni-trier.de/db/conf/memea/memea2023.html#KajiePSSMY23

  • Development of a Battery Using a Hydrogel Network

    Kohtaro Hongo, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022)   2022

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/BMEiCON56653.2022.10012086

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  • Enhancement of the Response to Ultrasound by Encapsulating Metal Nanoparticles in Liposomes

    Yukine Tagai, Kano Kajie, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Takashi Shibata, Tohru Yagi

    2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022)   2022

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/BMEiCON56653.2022.10012081

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  • Transmembrane Carbon Nanotubes for Intracellular Stimulation

    Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022)   2022

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/BMEiCON56653.2022.10012082

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  • Development of Variable-Elasticity Cell Scaffolds Using Magnetic Gels

    Yuya Shimomura, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022)   2022

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/BMEiCON56653.2022.10012083

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  • Self-spreading of lipid film on PDMS surface

    PENG ZUGUI, SHIMBA KENTA, MIYAMOTO YOSHITAKA, YAGI TOHRU

    JSAP Annual Meetings Extended Abstracts   2021.1   2148 - 2148   2021.2

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    Language：Japanese   Publisher：The Japan Society of Applied Physics  

    DOI： 10.11470/jsapmeeting.2021.1.0_2148

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  • Study on electrical properties of droplet networks for the development of biological batteries

    Hongo Kohtaro, Peng Zugui, Shimba Kenta, Miyamoto Yoshitaka, Yagi Tohru

    Transactions of Japanese Society for Medical and Biological Engineering   Annual59 ( Abstract )   433 - 433   2021

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    Language：Japanese   Publisher：Japanese Society for Medical and Biological Engineering  

    It is known that an electric current flows when ion-selective membrane proteins are inserted into the droplet interface bilayers (DIB). However, no method has been developed to control this current; therefore, we attempted to control the ionic current through the droplet network using carbon nanotubes (CNTs), which also have ion-selectivity. As a preliminary step, we have formed the DIB by binding droplets in lipids, and investigated the correlation between the membrane area and the capacitance of lipid bilayers in this research. The results show that there is a strong correlation between the membrane area and the capacitance, which verifies the usefulness of the experimental system. We have also measured the conductance of CNTs. A bimodal histogram has been obtained for the conductance indicating the insertion of CNTs into the lipid bilayer.

    DOI： 10.11239/jsmbe.annual59.433

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  • Study on the relationship between ultrasound exposure time and cellular drug uptake in DDS

    Kajie Kano, Peng Zugui, Yamamoto Yogo, Shimba Kenta, Miyamoto Yoshitaka, Yagi Tohru

    Transactions of Japanese Society for Medical and Biological Engineering   Annual59 ( Abstract )   571 - 571   2021

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    Language：Japanese   Publisher：Japanese Society for Medical and Biological Engineering  

    In the ultrasound-assisted drug delivery system (DDS), the target cells are irradiated with ultrasound waves to temporarily puncture the cell membrane, and at the same time, the drug is released from the nano-size liposomes which have the same structure as cell membrane and taken up by the cells. However, the relationship between drug uptake and ultrasound irradiation time has not been clarified. In this experiment, we irradiate artificial cell, which are micro-size liposomes, with ultrasound in a solution containing a fluorescent substance that we assume to be a drug. The results suggest that the uptake of the fluorescent substance increases as the irradiation time increases. In the future, we will conduct ultrasound irradiation experiments under other conditions, explore the relationship between drug uptake and each irradiation condition, and apply the results to DDS.

    DOI： 10.11239/jsmbe.annual59.571

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  • The effect of substrate hydrophilicity on lipid bilayer self-spreading

    PENG ZUGUI, SHIMBA KENTA, MIYAMOTO YOSHITAKA, YAGI TOHRU

    JSAP Annual Meetings Extended Abstracts   2020.1   2489 - 2489   2020.2

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    Language：Japanese   Publisher：The Japan Society of Applied Physics  

    DOI： 10.11470/jsapmeeting.2020.1.0_2489

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  • Patterning of supported lipid bilayer on hydrophilized PDMS substrate

    PENG ZUGUI, SHIMBA KENTA, MIYAMOTO YOSHITAKA, YAGI TOHRU

    JSAP Annual Meetings Extended Abstracts   2019.1   2535 - 2535   2019.2

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    Language：Japanese   Publisher：The Japan Society of Applied Physics  

    DOI： 10.11470/jsapmeeting.2019.1.0_2535

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  • Preparation of giant lipobeads using a gel- assisted swelling method

    Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    2019 12TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2019)   2019

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    Language：English   Publishing type：Research paper (international conference proceedings)  

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  • Nanopore-spanning lipid bilayer formed by self-spreading method

    Zugui Peng, Kenta Shimba, Yositaka Miyamoto, Tohru Yagi

    2018 11TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2018)   2018

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    Language：English   Publishing type：Research paper (international conference proceedings)  

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  • Effect of the amount of carbon nanotubes introduced into liposomes on membrane permeability

    Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi

    13TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2021)   2018

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/BMEiCON53485.2021.9745234

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Presentations

  • De Novo Designed α-helical Peptide Nanopores for Single-molecule Sensing Invited

    The 21st Beijing Conference and Exhibition on Instrumental Analysis  2025.9 

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    Presentation type：Oral presentation (invited, special)  

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  • 合成分子でナノポアを作る Invited

    生物物理学会サブグループランチセミナー  2024.1 

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    Presentation type：Oral presentation (invited, special)  

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  • 1分子センシングに向けた人工ナノポアの開発 Invited

    電気学会医用・生体工学研究会  2026.3 

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    Presentation type：Oral presentation (invited, special)  

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  • 大きな膜ペプチドナノポアを作る Invited

    第9回分子ロボティクス年次大会  2026.3 

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    Presentation type：Oral presentation (invited, special)  

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Industrial property rights

  • ペプチドシーケンシングに適用可能なde novoデザインナノポアタンパク質、及び情報処理方法

    川野 竜司, 竹内 七海, 彭 祖癸, 中田 彩夏, 内村 健治, 田中 良和

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    Application no：特願特願2025-088361  Date applied：2025.5

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Research Projects

  • DNAを材料とした環境応答型メカノドラ ッグデリバリーシステムの構築

    2026.10 - 2027.9

    戸部眞紀財団  研究助成金 

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  • 人工細胞の遠隔制御に向けた薄膜型機能デバイスの創出

    2026.8 - 2027.7

    村田学術振興・教育財団  萌芽的研究助成 自然科学 

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  • 長鎖ポリアミノ酸配列解析を志向した超長DNAナノ構造を有するナノポアの創出

    Grant number：26K17875  2026.4 - 2028.3

    日本学術振興会  科学研究費助成事業  若手研究

    彭 祖癸

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

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  • アミノ酸配列解析のためのDNA-ペプチドコンジュゲートナノポアの構築

    Grant number：24K17705  2024.4 - 2026.3

    日本学術振興会  科学研究費助成事業  若手研究

    彭 祖癸

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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  • DNAナノチューブを利用した高い生体適合性を有する細胞内刺激電極アレイの研究開発

    Grant number：21J14374  2021.4 - 2023.3

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    PENG ZUGUI

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    Grant amount：\1500000 （ Direct Cost: \1500000 ）

    本研究では、人工細胞膜とDNAナノチューブを用いた高い生体適合性を有する神経細胞内刺激電極アレイの開発を目指す。計画初年度にあたる本年度では，基盤技術の開発を行った。具体的には、1) ジメチルポリシロキサン（PDMS）上に高い機械強度を有する人工細胞膜の形成、2）長さが制御可能なDNAナノチューブの試作を行った。以下に，それぞれの詳細を述べる。
    1) PDMS上に高い機械強度を有する人工細胞膜の形成。ラズマ処理でPDMS表面を親水化処理させ、脂質の自発展開現象を利用して、PDMS表面に均一な脂質二重膜を形成した。形成した人工細胞膜は、PDMSにより支持されているため、高い機械的強度を有していると考えられる。また、プラズマ処理の時間を制御することにより、膜の形成速度や形成した膜の形状をコントロールできることがわかった。
    2) 長さが制御可能なDNAナノチューブの試作。DNA鎖置換反応を利用し、異なる長さを持つDNAナノチューブを結合させることで、DNAナノチューブの長さを制御する手法を開発した。原子間力顕微鏡を用いて形状を確認したところ、DNAナノチューブの長さは反応前34.4 nm、反応後64.2 nmで、長さを約2倍にすることができた。またDNAナノチューブの物質透過を評価するために、パッチクランプを用いた電気計測実験系を新たに構築した。結果、作製したDNAナノチューブが電荷を透過させ、導電性を持つことが判明した。

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  • 革新的なブレインマシンインターフェスの創出に向けた細胞内刺激電極の研究開発

    2021 - 2022

    電気通信普及財団  研究調査助成 

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  • DNAナノチューブのブレインマシンインターフェスへの応用の開拓

    2021 - 2022

    村田学術振興財団  研究調査助成 

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    Authorship：Principal investigator 

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  • 人工細胞膜と受容体タンパク質を利用した脳神経インタフェースの開発

    2020 - 2023

    立石科学技術振興財団  研究助成（C） 

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Academic Activities

  • 化学とマイクロ・ナノシステム学会53回研究会

    Role(s)： Planning, management, etc.

    2026

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  • The Nanopore Meeting 2025 in Tokyo

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    2025

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  • 化学とマイクロ・ナノシステム学会51回研究会

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    2025

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  • 化学とマイクロ・ナノシステム学会49回研究会

    Role(s)： Planning, management, etc.

    2024

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