Updated on 2026/04/29

写真a

 
OKAI NAOKO
 
Organization
School of Life Science and Technology Researcher
Title
Researcher
External link

Degree

  • 博士（農学） ( 東北大学 )

  • 修士（農学） ( 東北大学 )

Research Interests

  • 応用微生物学 生物工学 バイオマス利用 キシラナーゼ コリネ型細菌 バイオリファイナリー バイオマスプラスチック ビブリオ 鉄還元酵素 大腸菌 物質生産 酵母 膜輸送体

Research Areas

  • Life Science / Applied microbiology

Education

  • 東北大学大学院農学研究科 農芸化学専攻 博士後期課程

    1997.4 - 2000.3

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  • 東北大学大学院農学研究科 環境修復生物工学専攻 博士前期課程

    1995.4 - 1997.3

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  • 東北大学 農学研究科 農芸化学科

    1991.4 - 1995.3

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Papers

  • Microbbial production of aromatic compounds

    Naoko Okai

    Journal of.Antibacterial and Antifungal Agents   50 ( 1 )   33 - 37   2022

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • Iron-Utilization System in Vibrio vulnificus M2799 Reviewed International journal

    Katsushiro Miyamoto, Hiroaki Kawano, Naoko Okai, Takeshi Hiromoto, Nao Miyano, Koji Tomoo, Takahiro Tsuchiya, Jun Komano, Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Tsujibo

    Marine Drugs   19 ( 12 )   710 - 710   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Vibrio vulnificus is a Gram-negative pathogenic bacterium that causes serious infections in humans and requires iron for growth. A clinical isolate, V. vulnificus M2799, secretes a catecholate siderophore, vulnibactin, that captures ferric ions from the environment. In the ferric-utilization system in V. vulnificus M2799, an isochorismate synthase (ICS) and an outer membrane receptor, VuuA, are required under low-iron conditions, but alternative proteins FatB and VuuB can function as a periplasmic-binding protein and a ferric-chelate reductase, respectively. The vulnibactin-export system is assembled from TolCV1 and several RND proteins, including VV1_1681. In heme acquisition, HupA and HvtA serve as specific outer membrane receptors and HupB is a sole periplasmic-binding protein, unlike FatB in the ferric-vulnibactin utilization system. We propose that ferric-siderophore periplasmic-binding proteins and ferric-chelate reductases are potential targets for drug discovery in infectious diseases.

    DOI： 10.3390/md19120710

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  • VuuB and IutB reduce ferric-vulnibactin in Vibrio vulnificus M2799. Reviewed International journal

    Naoko Okai, Katsushiro Miyamoto, Koji Tomoo, Takahiro Tsuchiya, Jun Komano, Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Tsujibo

    Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine   33 ( 4-5 )   187 - 200   2020.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Vibrio vulnificus, a pathogenic bacterium that causes serious infections in humans, requires iron for growth. Clinical isolate, V. vulnificus M2799, secretes a catecholate siderophore, namely, vulnibactin, to capture iron (III) from the environment. Growth experiments using a deletion mutant indicated that VuuB, a member of the FAD-containing siderophore-interacting protein family, plays a crucial role in Fe3+-vulnibactin reduction. IutB, a member of the ferric-siderophore reductase family, stands a substitute for VuuB in its absence. It remained unclear why V. vulnificus M2799 has two proteins with relevant functions. Here we biochemically characterized VuuB and IutB using purified recombinant proteins. Purified VuuB, a flavoprotein, catalyzed the reduction of Fe3+-nitrilotriacetic acid as its electron acceptor, in the presence of NADH as its electron donor and FAD as its cofactor. IutB catalyzed the reduction of Fe3+-nitrilotriacetic acid, in the presence of NADH, NADPH, or reduced glutathione as its electron donor. The optimal pH values and temperatures of VuuB and IutB were 7.0 and 37 °C, and 8.5 and 45 °C, respectively. On analyzing their ferric-chelate reductase activities, both VuuB and IutB were found to catalyze the reduction of Fe3+-aerobactin, Fe3+-vibriobactin, and Fe3+-vulnibactin. When the biologically relevant substrate, Fe3+-vulnibactin, was used, the levels of ferric-chelate reductase activities were similar between VuuB and IutB. Finally, the mRNA levels were quantified by qRT-PCR in M2799 cells cultivated under low-iron conditions. The number of vuuB mRNA was 8.5 times greater than that of iutB. The expression ratio correlated with the growth of their mutants in the presence of vulnibactin.

    DOI： 10.1007/s10534-020-00241-5

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  • Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase. Reviewed International journal

    Naoko Okai, Takaya Masuda, Yasunobu Takeshima, Kosei Tanaka, Ken-Ichi Yoshida, Masanori Miyamoto, Chiaki Ogino, Akihiko Kondo

    AMB Express   7 ( 1 )   130 - 130   2017.12

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    Authorship：Lead author   Language：English  

    Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.

    DOI： 10.1186/s13568-017-0427-9

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  • Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system. Reviewed International journal

    Kengo Sasaki, Daisuke Sasaki, Naoko Okai, Kosei Tanaka, Ryohei Nomoto, Itsuko Fukuda, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa

    PloS one   12 ( 7 )   e0180991   2017

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    Language：English  

    Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects.

    DOI： 10.1371/journal.pone.0180991

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  • Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli. Reviewed International journal

    Naoko Okai, Takanori Miyoshi, Yasunobu Takeshima, Hiroaki Kuwahara, Chiaki Ogino, Akihiko Kondo

    Applied microbiology and biotechnology   100 ( 1 )   135 - 45   2016.1

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    Authorship：Lead author   Language：English  

    Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway for PCA from glucose was engineered in Corynebacterium glutamicum. The pathway to PCA-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (CPL) and 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL in a phenylalanine-producing strain of C. glutamicum ATCC21420. To secrete PCA, the gene (ubiC) encoding CPL from E. coli was expressed in C. glutamicum ATCC 21420 (strain F(UbiC)). The formation of 28.8 mg/L of extracellular 4-HBA (36 h) and 213 ± 29 mg/L of extracellular PCA (80 h) was obtained by the C. glutamicum strain F(UbiC) from glucose. The strain ATCC21420 was also found to produce extracellular PCA. PCA fermentation was performed using C. glutamicum strain F(UbiC) in a bioreactor at the optimized pH of 7.5. C. glutamicum F(UbiC) produced 615 ± 2.1 mg/L of PCA from 50 g/L of glucose after 72 h. Further, fed-batch fermentation of PCA by C. glutamicum F(UbiC) was performed with feedings of glucose every 24 h. The maximum production of PCA (1140.0 ± 11.6 mg/L) was achieved when 117.0 g/L of glucose was added over 96 h of fed-batch fermentation.

    DOI： 10.1007/s00253-015-6976-4

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  • 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum Reviewed

    Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    BIORESOURCE TECHNOLOGY   198   410 - 417   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2015.09.024

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  • Development of bio-based fine chemical production through synthetic bioengineering Reviewed

    Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko Kondo

    MICROBIAL CELL FACTORIES   13 ( 1 )   173   2014.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12934-014-0173-5

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  • Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase Reviewed

    Naoko Okai, Chihiro Takahashi, Kazuki Hatada, Chiaki Ogino, Akihiko Kondo

    AMB EXPRESS   4   20   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s13568-014-0020-4

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  • Thermal Stability and Starch Degradation Profile of alpha-Amylase from Streptomyces avermitilis Reviewed

    Sang Youn Hwang, Kazunori Nakashima, Naoko Okai, Fumiyoshi Okazaki, Michiru Miyake, Koichi Harazono, Chiaki Ogino, Akihiko Kondo

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 12 )   2449 - 2453   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1271/bbb.130556

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  • バイオマスプラスチックとバイオリファイナリー

    OKAI NAOKO, KONDO AKIHIKO

    配管技術   55 ( 7 )   30 - 35   2013.6

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    Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • P-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans Reviewed

    Yoshifumi Kawai, Shuhei Noda, Chiaki Ogino, Yasunobu Takeshima, Naoko Okai, Tsutomu Tanaka, Akihiko Kondo

    Microbial Cell Factories   12 ( 1 )   45   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/1475-2859-12-45

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  • A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology Reviewed

    Tomohisa Hasunuma, Fumiyoshi Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo

    BIORESOURCE TECHNOLOGY   135   513 - 522   2013.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.biortech.2012.10.047

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  • Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli Reviewed

    Chihiro Takahashi, Junki Shirakawa, Takeyuki Tsuchidate, Naoko Okai, Kazuki Hatada, Hideki Nakayama, Toshihiro Tateno, Chiaki Ogino, Akihiko Kondo

    ENZYME AND MICROBIAL TECHNOLOGY   51 ( 3 )   171 - 176   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.enzmictec.2012.05.010

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  • Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae Reviewed

    Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   93 ( 4 )   1495 - 1502   2012.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-011-3665-9

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  • バイオリファイナリーとバイオプラスチック

    OKAI NAOKO, HASUNUMA TOMOHISA, KONDO AKIHIKO

    ペトロテック   35 ( 10 )   700 - 706   2012

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  • Characterization of the mannitol catabolic operon of Corynebacterium glutamicum Reviewed

    Xue Peng, Naoko Okai, Alain A. Vertes, Ken-ichi Inatomi, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   91 ( 5 )   1375 - 1387   2011.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-011-3352-x

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  • Glutamate production from beta-glucan using endoglucanase-secreting Corynebacterium glutamicum Reviewed

    Takeyuki Tsuchidate, Toshihiro Tateno, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   90 ( 3 )   895 - 901   2011.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-011-3116-7

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  • Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase Reviewed

    Shuhei Noda, Takaya Miyazaki, Takanori Miyoshi, Michiru Miyake, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY   38 ( 5 )   643 - 648   2011.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10295-011-0955-2

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  • Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans Reviewed

    Chiaki Ogino, Shuhei Noda, Akihiko Kondo, Naoko Okai, Tsutomu Tanaka

    2011 Defense Science Research Conference and Expo, DSR 2011   2011

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.1109/DSR.2011.6026816

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  • Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R Reviewed

    Yuya Tanaka, Naoko Okai, Haruhiko Teramoto, Masayuki Inui, Hideaki Yukawa

    MICROBIOLOGY-SGM   154   264 - 274   2008.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/mic.0.2007/008862-0

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  • Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R Reviewed

    Hideaki Yukawa, Crispinus A. Omumasaba, Hiroshi Nonaka, Peter Kos, Naoko Okai, Nobuaki Suzuki, Masako Suda, Yota Tsuge, Junko Watanabe, Yoko Ikeda, Alain A. Vertes, Masayuki Inui

    MICROBIOLOGY-SGM   153   1042 - 1058   2007.4

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    DOI： 10.1099/mic.0.2006/003657-0

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  • High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library Reviewed

    N Suzuki, N Okai, H Nonaka, Y Tsuge, M Inui, H Yukawa

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   72 ( 5 )   3750 - 3755   2006.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/AEM.72.5.3750-3755.2006

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  • Corynebacterium glutamicum glyceraldehyde-3-phosphate dehydrogenase isoforms with opposite, ATP-dependent regulation Reviewed

    CA Omumasaba, N Okai, M Inui, H Yukawa

    JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY   8 ( 2 )   91 - 103   2004

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1159/000084564

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  • Cloning, expression, and cell surface localization of Paenibacillus sp strain W-61 xylanase 5, a multidomain xylanase Reviewed

    Y Ito, T Tomita, N Roy, A Nakano, N Sugawara-Tomita, S Watanabe, N Okai, N Abe, Y Kamio

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   69 ( 12 )   6969 - 6978   2003.12

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    DOI： 10.1128/AEM.69.12.6969-6978.2003

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  • Purification, Characterization and Gene Cloning of High-molecular-weight xylanases 4 of A. caviae W-61.

    NARAYAN Roy, OKAI Naoko, KAMIO Yoshiyuki

    Pakistan Journal of Biological Sciences   4 (8), 1006-1011.   2001

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  • 大豆プロテオリピド-アポ蛋白質とオレオシンの分子構造的相関性 Reviewed

    SUZUKI Miyoko, NAKAMURA Tomoyuki, OKAI Naoko, OGAWA Tomihisa, MURAMOTO Koji

    大豆たん白質研究   (4), p27-32 ( 22 )   27 - 32   2001

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • Purification and some properties of high-molecular-weight xylanases, the xylanases 4 and 5 of Aeromonas caviae W-61 Reviewed

    N Roy, N Okai, T Tomita, K Muramoto, Y Kamio

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   64 ( 2 )   408 - 413   2000.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1271/bbb.64.408

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  • Molecular properties and activity of a carboxyl-terminal truncated form of xylanase 3 from Aeromonas caviae W-61 Reviewed

    N Okai, M Fukasaku, J Kaneko, T Tomita, K Muramoto, Y Kamio

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   62 ( 8 )   1560 - 1567   1998.8

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    DOI： 10.1271/bbb.62.1560

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Books

  • 化学便覧 応用化学編

    KONDO AKIHIKO, OGINO CHIAKI, HASUNUMA TOMOHISA, ISHII JUN, HARA KIYOTAKA, OKAI NAOKO, 山田 亮祐（ Role： Joint author）

    丸善出版  2014.1 

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    Language：Japanese   Book type：Scholarly book

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MISC

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Presentations

  • Construction of epsilon-caprolactam biosynthesis pathway in Escherichia coli.

    Yuto Goto, Naoko Okai, Mitsuru Haruki, Nobutaka Hirano

    2025.9 

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    Event date： 2025.9

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  • Analysis of synergic effect of Clostridium thermocellum cellulosome.

    Masahiro Watanabe, Kodai Tsurui, Naoko Okai, Mitsuru Haruki, Nobutaka Hirano

    2025.9 

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    Event date： 2025.9

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  • Counterselection system for deletion of cellulosomal gene in Clostridium thermocellum.

    Naoko Okai, Takuro Tomita, Mitsuru Haruki, Nobutaka Hirano

    令和7年度化学系学協会東北大会  2025.9 

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  • 好熱嫌気性細菌由来セルロソームの相乗作用解析

    渡部 真大, 鶴井 皓大, 岡井 直子, 春木 満, 平野 展孝

    令和6年度日本大学工学部学術研究報告会  2024.12 

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    Event date： 2024.12

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 6-ナイロン原料生合成オペロンの構築

    後藤 優斗, 岡井 直子, 春木 満, 平野 展孝

    令和6年度日本大学工学部学術研究報告会  2024.12 

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    Event date： 2024.12

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  • 大腸菌における細菌微小区画の形成と緑色蛍光タンパク質の殻内発現

    渡邉 祐哉, 岡井 直子, 橋本 優允子, 上野 俊吉, 岸 努, 春木 満, 平野 展孝

    第66回日本大学工学部学術研究報告会  2023.12 

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    Event date： 2023.12

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • [FeFe]ヒドロゲナーゼの再構成

    鷺沼 璃樹, 岡井 直子, 春木 満, 平野 展孝

    第66回日本大学工学部学術研究報告会  2023.12 

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    Event date： 2023.12

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  • Construction of disruption mutant of cellulosomal endoglucanase gene in Clostridium thermocellum.

    Takuro Tomita, Naoko Okai, Mitsuru Haruki, Nobutaka Hirano

    2023.9 

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    Event date： 2023.9

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  • Encapsulation of a multienzyme complex into a bacterial microcompartment in Escherichia coli cells.

    Watanabe, Rui Sasaki, Kazuki Ishiyama, Naoko Okai, Tsutomu Kishi, Mitsuru Haruki, Nobutaka Hirano

    2023.9 

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  • Genomic gene integration mediated by serine-type phage integrases.

    Yuki Murata, Naoko Okai, Mitsuru Haruki, Nobutaka Hirano

    2023.9 

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  • Analysis of synergistic effect generated by Clostridium thermocellum cellulosome.

    Kodai Tsurui, Naoko Okai, Mitsuru Haruki, Nobutaka Hirano

    2023.9 

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  • コリネ型細菌を用いたプロトカテク酸の生産

    OKAI NAOKO, 竹嶋 康誠, OGINO CHIAKI, KONDO AKIHIKO

    第65回日本生物工学会大会  2013.9  日本生物工学会

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    Venue：広島市  

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  • コリネ型細菌を用いた糖を原料とするGABAの生産

    OKAI NAOKO, 高橋 千尋, 畑田 一樹, OGINO CHIAKI, KONDO AKIHIKO

    日本農芸化学会2014年度大会  2014.3  日本農芸化学会

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    Venue：川崎市  

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  • コリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産

    岡井 直子, 増田 敬也, 竹嶋 康誠, 田中 耕生, 吉田 健一, 桑原 広明, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2017年度大会  2017.3 

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  • Vibrio vulnificus M2799 株の鉄獲得機構の解明

    宮本 勝城, 河野 広明, 岡井 直子, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    第38回 日本循環制御医学会総会・学術集会  2017.6 

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  • Vibrio vulnificus M2799 株の鉄獲得機構の解明

    宮本 勝城, 岡井 直子, 河野 広明, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    第29回微生物シンポジウム  2017.8 

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  • Vibrio vulnificus M2799 株の鉄還元酵素 VuuB の諸性質

    岡井 直子, 宮本 勝城, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    日本薬学会第139年会  2019.3 

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  • Ferric utilization system in Vibrio vulnificus M2799.

    K. Miyamoto, H. Kawano, N. Okai, T. Hiromoto, N. Miyano, K. Tomoo, T. Tsuchiya, J. Komano, T. Tanabe, T. Funahashi, H. Tsujibo

    World Microbe Forum  2021.6 

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  • Vibrio vulnificus M2799 株の鉄獲得機構の解明

    宮本 勝城, 河野 広明, 岡井 直子, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    第41回日本鉄バイオサイエンス学会学術集会  2017.9 

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  • Vibrio vulnificus M2799 株の鉄獲得機構の解明

    宮本 勝城, 岡井 直子, 河野 広明, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    第67回日本薬学会近畿支部総会・大会  2017.10 

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  • Vibrio vulnificus M2799 株のヘム獲得機構の解明

    宮本 勝城, 岡井 直子, 河野 広明, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    日本薬学会第138年会  2018.3 

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  • Vibrio vulnificus M2799 株の鉄獲得機構の解明

    宮本 勝城, 河野 広明, 岡井 直子, 土屋 孝弘, 田邊 知孝, 舟橋 達也, 辻坊 裕

    第 42 回日本鉄バイオサイエンス学会学術集会  2018.9 

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  • Aeromonas caviae W-61のXylanase3遺伝子のクローニング

    岡井 直子, 深作 昌士, 村本 光二, 金子 淳, 富田 敏夫, 神尾 好是

    日本農芸化学会 東北・北海道合同支部 1997年  1997.9 

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  • Aeromonas caviae W-61のキシラナーゼ3のC末端欠損変異体の基質特異性

    OKAI Naoko, FUKASAKU Masashi, NARAYAN Roy, KANEKO Jun, TOMITA Toshio, MURAMOTO Koji, KAMIO Yoshiyuki

    日本農芸化学会 1999年度大会  1999.3  日本農芸化学会

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    Venue：福岡市  

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  • ウナギ目体表粘液由来レクチンの精製と分子特性の解析

    SANNOHE Takanori, TATENO Hiroaki, OKAI Naoko, OGAWA Tomihisa, MURAMOTO Koji, HATTORI Masanori

    日本生物化学会大会 2002年度  2002.10  日本生物化学会

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    Venue：京都市  

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  • グラム陰性細菌Aeromonas caviae W-61の分泌型キシラナーゼおよび細胞結合型キシラナーゼ

    KAMIO Yoshiyuki, TOMITA Toshio, ABE Naoki, Nugyen Viet Dung, OKAI Naoko, NARAYAN Roy, ITO Yasuko

    日本生物工学会 平成14年 54回大会  2002.10  日本生物工学会

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    Venue：大阪市  

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  • Aeromonas caviae W-61株の産生するxylanase1の糖転移活性および遺伝子構造の解析

    WATABE Seiji, Nguyen V.Dung, OKAI Naoko, KANEKO Jun, TOMITA Toshio, KAMIO Yoshiyuki

    日本農芸化学会 2003年度大会  2003.4  日本農芸化学会

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    Venue：藤沢市  

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  • Corynebacterium glutamicum R 株のゲノム解析

    NONAKA Hiroshi, NAKATA Kaori, OKAI Naoko, WADA Mariko, SATO Yumiko, KOS Peter, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2003年度大会  2003.4  日本農芸化学会

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    Venue：藤沢市  

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  • Isolation, Characterization and Gene Cloning of Xylanase 5 from Aeromonas caviae W-61

    Narayan Roy, Toshio Tomita, OKAI Naoko, Koji Muramoto, Yoshiyuki Kamio

    日本農芸化学会 1999年度大会  1999.3  日本農芸化学会

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    Venue：福岡市  

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  • Aeromonas caviae W-61の産生するキシラナーゼ3、キシラナーゼ5の機能と構造

    OKAI Naoko, NARAYAN Roy, TOMITA Toshio, MURAMOTO Koji, KAMIO Yoshiyuki

    日本農芸化学会 東北・北海道 合同支部会  1999.10  日本農芸化学会

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    Venue：札幌市  

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  • Aeromonas caviae W-61株の産生する高分子量キシラナーゼ ( Xyn5 )の遺伝子クローニング及びC末端領域の解析

    ITO Yasuko, NARAYAN Roy, OKAI Naoko, ABE Naoki, TOMITA Toshio, YOSHIDA Shigeki, KAMIO Yoshiyuki

    日本農芸化学会 2001年度大会  2001.3  日本農芸化学会

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    Venue：京都市  

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  • コリネ型細菌を用いた新規バイオプロセスの開発: 代謝制御機構の解析

    OKINO Shohei, KAWAGUCHI Hideo, OKAI Naoko, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2003年度大会  2003.4  日本農芸化学会

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    Venue：藤沢市  

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  • Production of lactic acid by novel bioprocess using coryneform bacteria. International conference

    Okino Shohei, Nakata Kaori, Okai Naoko, Inui Masayuki, Omumasaba Crispinus.A, Yukawa Hideaki

    National Renewable Energy Laboratory,The 25th Symposium on Biotechnology for Fuels and Chemicals  2003.5  National Renewable Energy Laboratory

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    Venue：Breckenridge  

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  • Physiological Effects of GAPDH disruption in Corynebacterium glutamicum.

    OMUMASABA Crispinus, OKAI Naoko, WADA Mariko, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2004年度大会  2004.3  日本農芸化学会

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    Venue：東広島市  

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  • コリネ型細菌の糖輸送系( PTS )遺伝子破壊株の解析 (2)

    OKAI Naoko, SUZUKI Nobuaki, IKEDA Yoko, NONAKA Hiroshi, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2005年度大会  2005.3  日本農芸化学会

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    Venue：札幌市  

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  • Genome engineering and analysis for Corynebacterium glutamicum. International conference

    Suzuki Nobuaki, Nonaka Hiroshi, Okai Naoko, Tsuge Yota, Inui Masayuki, Yukawa Hideaki

    BIO, ACS, NABC, The 2nd Annual World Congress on Industrial Biotechnology and Bioprocessing  2005.4  American Chemical Society

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    Venue：Orland  

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  • Genome engineering and analysis of Corynebacterium glutamicum. International conference

    Inui Masayuki, Suzuki Nobuaki, Tsuge Yota, Okai Naoko, Suda Masako, Vertes Alain A, Yukawa Hideaki

    Zentiva IVAX, FEMS, 10th International Congress on the Genetics of Industrial Microorganisms  2006.6  FEMS

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    Venue：Prague, Czech  

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  • コリネ型細菌におけるPTS遺伝子の発現解析

    TANAKA Yuya, OKAI Naoko, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2007年度大会  2007.3  日本農芸化学会

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    Venue：東京都  

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  • コリネ型細菌の糖輸送系(PTS)遺伝子破壊株の構築と解析

    OKAI Naoko, NONAKA Hiroshi, IKEDA Yoko, WADA Mariko, SUZUKI Nobuaki, INUI Masayuki, YUKAWA Hideaki

    日本農芸化学会 2004年度大会  2004.3  日本農芸化学会

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    Venue：東広島市  

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  • Analysis of sugar utilization by Corynebacterium glutamicum. International conference

    Murakami Shikiko, Okai Naoko, Suda Masako, Inui Masayuki, Yukawa Hideaki

    ACS, 228th National Meeting  2004.8  American Chemical Society

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    Venue：Philadelphia  

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  • High throughput transposon-mediated mutagenesis and construction of disruptant library of Corynebacterium glutamicum」 International conference

    Nobuaki Suzuki, Naoko Okai, Hiroshi Nonaka, Yota Tsuge, Masayuki Inui, Hideaki Yukawa

    RITE国際シンポジウム、International Workshop on Biorefinery  2005.2  RITE

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    Venue：京都市  

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  • The complete genome sequence of Corynebacterium glutamicum R and the comparative genome analysis with other corynebacteria International conference

    Hiroshi Nonaka, Peter Kos, Naoko Okai, Masayuki Inui, Hideaki Yukawa

    RITE国際シンポジウム、International Workshop on Biorefinery  2005.2  RITE

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    Venue：京都市  

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  • Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase System (PTS) genes in Corynebacterium glutamicum R. International conference

    Tanaka Yuya, Okai Naoko, Teramoto Haruhiko, Inui Masayuki, Yukawa Hideaki

    American Society for Microbiology, 107th General Meeting  2007.5  American Society for Microbiology

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    Venue：Toronto, Canada  

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  • 網羅的表現型解析を基盤とした実用パン酵母の分子育種.

    ANDO Akira, HAMASAKO Akiko, OOYA Setsuko, OKAI Naoko, TAKAGI Hiroshi, SHIMA Jun

    日本農芸化学会 2009年度大会  2009.3  日本農芸化学会

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    Venue：福岡市  

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  • コリネ型細菌を用いた澱粉系バイオマスの利用

    OKAI NAOKO, 足立 典子, 高橋 千尋, NIBA Emma, MATSUDA FUMIO

    日本農芸化学会2013年度大会  2013.3  日本農芸化学会

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    Venue：仙台市  

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  • Porin を用いたコリネ菌新規細胞表層提示技術の開発

    畑田 一樹, 舘野 俊博, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    第61回日本生物工学会大会  2009.9  (社)日本生物工学会

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    Venue：名古屋市  

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  • 放線菌によるトランスグルタミナーゼの生産

    宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    化学工学会第15回学生発表会  2011.3  (社)化学工学会

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    Venue：神戸市  

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  • 放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討

    宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    日本農芸化学会2011年度大会  2011.3  日本農芸化学会

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    Venue：京都市  

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  • 放線菌を用いたあらゆる炭素源からのケイ皮酸生産

    野田 修平, 三好 孝則, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    化学工学会第76年会  2011.3  日本化学会

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    Venue：東京都 小金井市  

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  • Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividans Invited International conference

    NODA Shuhei, MIYOSHI, Takanori, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    33rd Symposium on Biotechnology for Fuels and Chemicals  2011.5  Society for industrial Microbiology

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    Venue：Seattle, USA  

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  • 放線菌を用いた様々なタンパクの大量分泌系の構築

    野田 修平, 岡井 直子, 田中 勉, 近藤 昭彦, 荻野 千秋

    化学工学会第75年会  2010.3  (社)化学工学会

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    Venue：鹿児島市  

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  • Corynebacterium glutamicumを用いたGABAの高効率生産技術の開発

    高橋 千尋, 白川 順規, 畑田 一樹, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    化学工学会第15回学生発表会  2011.3  (社)化学工学会

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    Venue：神戸市  

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  • コリネ型細菌を用いたGABAの生産

    高橋 千尋, 白川 順規, 畑田 一樹, 土舘 健幸, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    日本農芸化学会2011年度大会  2011.3  日本農芸化学会

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    Venue：京都市  

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  • 放線菌における表層提示技術に関する研究

    中村 悠佑, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    化学工学会第15回学生発表会  2011.3  (社)化学工学会

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    Venue：神戸市  

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  • Corynebacterium glutamicumを用いた高効率なGABA生産技術の開発

    高橋 千尋, 白川 順規, 畑田 一樹, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第43回秋季大会  2011.9  化学工学会

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    Venue：名古屋市  

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  • コリネ型細菌を用いたプトレシンの生産

    岡井 直子, 畑田 一樹, 高橋 千尋, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2012年度大会  2012.3  日本農芸化学会

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    Venue：京都市  

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  • コリネ型細菌を用いた糖を原料とするカダベリンの生産

    OKAI NAOKO, NIBA Emma, 仲山 英樹, MATSUDA FUMIO

    第64回日本生物工学会大会  2012.10  日本生物工学会

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    Venue：神戸市  

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  • Production of four-carbon building block of bio-plastics by engineered Corynebacterium glutamicum.

    Naoko Okai, Chihiro Takahashi, Kazuki Hatada, Chiaki Ogino, Akihiko kondo

    GRIP2014  2014.3 

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  • 放線菌を用いた糖を原料とするパラアミノ安息香酸の生産

    OKAI NAOKO, 佐藤 嘉弘, 大野 摩耶, 竹嶋 康誠, 増田 敬哉, 宮本 正教, 樋田 幸三, OGINO CHIAKI, KONDO AKIHIKO

    第66回日本生物工学会大会  2014.9  日本生物工学会

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    Venue：札幌市  

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  • コリネ型細菌を用いたプロトカテク酸の生産

    OKAI NAOKO, 竹嶋 康誠, OGINO CHIAKI, KONDO AKIHIKO

    日本農芸化学会2015年度大会  2015.3  日本農芸化学会

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    Venue：岡山市  

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  • コリスミ酸ピルビン酸リアーゼ発現コリネ型細菌を用いたプロトカテク酸の生産

    岡井 直子, 三好 孝則, 竹嶋 康誠, 田中 耕生, 吉田 健一, 桑原 広明, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会  2016.9 

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  • β- グルコシダーゼ発現Corynebacterium glutamicum を用いたセロビオースからのリジン生産

    足立 典子, 小野 尚子, 平田 有希, 高橋 千尋, OKAI NAOKO, 田中 勉, KONDO AKIHIKO

    第64回日本生物工学会大会  2012.10  日本生物工学会

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    Venue：神戸市  

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