Updated on 2026/03/26

写真a

 
LIU ZIZHEN
 
Organization
School of Materials and Chemical Technology Assistant Professor
Title
Assistant Professor
External link

Degree

  • Doctor of Engineering ( Nagaoka University of Technology )

Research Interests

  • 生体材料工学

  • ナノバイオ材料

  • 無機材料化学

Research Areas

  • Nanotechnology/Materials / Nanomaterials

  • Nanotechnology/Materials / Nanometer-scale chemistry

  • Nanotechnology/Materials / Composite materials and interfaces

Papers

  • Self-Assembled Gel-like Aggregation States of Calcium Phosphate Nanoparticles. International journal

    Wanyu Shi, Kurisu Mikami, Iori Yamada, Justian Chandra, Zizhen Liu, Yuhi Kinoshita, Motohiro Tagaya

    Inorganic chemistry   65 ( 1 )   191 - 199   2026.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    We proposed the fabrication of a gel composed primarily of calcium phosphate (CP) nanoparticles (NPs). Here, the additive of citric acid (Citr), which is an organic molecule present in bone tissues that chelates with calcium ions, was investigated during the synthesis of the CP NPs, with a focus on a gel-like aggregation state. As a result, the Citr-coordinated CP NP (Citr/CP NP) sols exhibiting different thermal responses ((1) maintenance of a sol state, (2) formation of a stable gel-like aggregation state, and (3) formation of a thixotropic gel-like aggregation state) were successfully prepared by controlling the nucleation and nanoparticle growth temperatures during the synthesis. Specifically, the sol of the highly anisotropic Citr/CP NP nucleated at 40 °C maintained its sol state after heat at 37.5 °C, whereas the isotropic Citr/CP NP at 4 °C formed the gel-like aggregation state upon the heat. The sol grown at 4 °C formed a stable gel-like aggregation state that was resistant to external force, whereas that at 40 °C exhibited thixotropy, which is a property commonly observed in synovial fluid. The gelation would be attributed to the precipitation of calcium citrate tetrahydrate, which exhibited retrograde solubility and promoted cross-linking among the CP NPs.

    DOI： 10.1021/acs.inorgchem.5c03933

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  • Self-Assembled Gel-like Aggregation States of Calcium Phosphate Nanoparticles

    Wanyu Shi, Kurisu Mikami, Iori Yamada, Justian Chandra, Zizhen Liu, Yuhi Kinoshita, Motohiro Tagaya

    INORGANIC CHEMISTRY   65 ( 1 )   191 - 199   2026.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.inorgchem.5c03933

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  • Hydroxyapatite nanoparticle films with designed nanostructures for advanced cell culture. International journal

    Zizhen Liu, Daichi Noda, Wanyu Shi, Tania Guadalupe Peñaflor Galindo, Yuichi Otsuka, Motohiro Tagaya

    Nanoscale   17 ( 48 )   27802 - 27825   2025.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Tissue engineering technology can be used to reconstruct biological tissues or organs with lesions or loss of function and minimize the damage to patients during the repair process. As an important and fundamental component of tissue engineering, the development of cell culture technology has attracted widespread attention. In particular, the design of cell culture substrates that can provide good cell culture properties has been extensively studied. The main commercially available culture substrate is tissue culture polystyrene (TCPS), but the survival rate of cells cultured on TCPS after in vivo transplantation is relatively low, which is considered to result from the environmental differences in composition and structure between TCPS and biological tissues. Accordingly, we summarized the current status of cell culture, the issues associated with culture substrates, and the conditions required for good cell culture substrates. In particular, the culture substrate needs to be highly transparent for optical microscopy. Moreover, since cell adhesion occurs by recognizing the proteins adsorbed on the substrate, it is proposed to control the protein adsorption state through nanostructures. Thus, hydroxyapatite (HA) with good biocompatibility has attracted attention, and its properties and the possibility of forming a transparent nanostructured film have been summarized. Furthermore, based on the inorganic-organic composite structures in living bodies, such as bone tissue, the coordination between HA nanoparticles and organic citric acid molecules has also been considered to fabricate functional films for producing new cell culture substrates with a wider range of applications. In this review, the basis of cell culture technology is systematically explained, and the design concepts of culture substrate surfaces for achieving favorable cell culture properties are highlighted. Surface functionalization of HA nanoparticle films is proposed to enhance protein adsorption and cell adhesion, and is expected to have wide applications in the field of regenerative medicine.

    DOI： 10.1039/d5nr02718g

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  • Confinement-Controlled Formation of Calcium Phosphate Clusters within Iron Porphyrin-Functionalized Oriented Mesoporous Silica Nanospaces

    Reo Kimura, Nozomi Ehira, Zizhen Liu, Yuhi Kinoshita, Kenichiro Kosugi, Nozomi Emura, Motohiro Tagaya

    JOURNAL OF PHYSICAL CHEMISTRY C   129 ( 45 )   20362 - 20372   2025.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.jpcc.5c05409

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  • Preparation of Octacalcium Phosphate Thin Film with Exposing Reactive Crystalline Plane in Biological Fluid

    Yanni Zhou, Zizhen Liu, Daichi Noda, Iori Yamada, Motohiro Tagaya

    ACS BIOMATERIALS SCIENCE & ENGINEERING   11 ( 2 )   1150 - 1160   2025.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsbiomaterials.4c02011

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  • Immobilization of cationic dye on photoluminescent hydroxyapatite particles through a citric acid bonding layer

    Daichi Noda, Wanyu Shi, Aiga Yamada, Zizhen Liu, Motohiro Tagaya

    REACTION CHEMISTRY & ENGINEERING   9 ( 11 )   2863 - 2867   2024.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d4re00277f

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  • Surface functionalization of hydroxyapatite nanoparticles for biomedical applications

    Takuya Kataoka, Zizhen Liu, Iori Yamada, Tania Guadalupe Penaflor Galindo, Motohiro Tagaya

    JOURNAL OF MATERIALS CHEMISTRY B   12 ( 28 )   6805 - 6826   2024.7

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  • Biological Surface Layer Formation on Bioceramic Particles for Protein Adsorption. International journal

    Reo Kimura, Daichi Noda, Zizhen Liu, Wanyu Shi, Ryota Akutsu, Motohiro Tagaya

    Biomimetics (Basel, Switzerland)   9 ( 6 )   2024.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    In the biomedical fields of bone regenerative therapy, the immobilization of proteins on the bioceramic particles to maintain their highly ordered structures is significantly important. In this review, we comprehensively discussed the importance of the specific surface layer, which can be called "non-apatitic layer", affecting the immobilization of proteins on particles such as hydroxyapatite and amorphous silica. It was suggested that the water molecules and ions contained in the non-apatitic layer can determine and control the protein immobilization states. In amorphous silica particles, the direct interactions between proteins and silanol groups make it difficult to immobilize the proteins and maintain their highly ordered structures. Thus, the importance of the formation of a surface layer consisting of water molecules and ions (i.e., a non-apatitic layer) on the particle surfaces for immobilizing proteins and maintaining their highly ordered structures was suggested and described. In particular, chlorine-containing amorphous silica particles were also described, which can effectively form the surface layer of protein immobilization carriers. The design of the bio-interactive and bio-compatible surfaces for protein immobilization while maintaining the highly ordered structures will improve cell adhesion and tissue formation, thereby contributing to the construction of social infrastructures to support super-aged society.

    DOI： 10.3390/biomimetics9060347

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  • Control of Biological Surface States on Chlorine-Doped Amorphous Silica Particles and Their Effective Absorptive Ability for Antibody Protein

    Reo Kimura, Sunao Chatani, Masahiko Inui, Satoshi Motozuka, Zizhen Liu, Motohiro Tagaya

    LANGMUIR   40 ( 17 )   8939 - 8949   2024.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.langmuir.4c00114

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  • Preparation of hydroxyapatite nanoparticle-hyaluronic acid hybrid membranes through citric acid molecular mediation

    Aoi Endo, Zizhen Liu, Daichi Noda, Mari Miyata, Motohiro Tagaya

    MATERIALS ADVANCES   5 ( 5 )   1887 - 1891   2024.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d3ma00882g

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  • Adsorption of tris(8-hydroxyquinolinato)aluminum on phosphoric acid-containing mesoporous silica particles

    Wanyu Shi, Zizhen Liu, Takuya Kataoka, Yuri Kurakami, Motohiro Tagaya

    MATERIALS CHEMISTRY AND PHYSICS   297   2023.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.matchemphys.2023.127334

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  • Effective Thermal Diffusion of Eu(III) and F Ions into Hydroxyapatite Nanoparticles by Citric Acid Coordinative Mediation

    Wanyu Shi, Zizhen Liu, Iori Yamada, Daichi Noda, Takuya Kataoka, Kenji Shinozaki, Motohiro Tagaya

    ACS APPLIED NANO MATERIALS   2023.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsanm.2c04358

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  • Analytical investigation of nano-bio interfacial protein mediation for fibroblast adhesion on hydroxyapatite nanoparticles

    Zizhen Liu, Yanni Zhou, Reo Kimura, Motohiro Tagaya

    PHYSICAL CHEMISTRY CHEMICAL PHYSICS   25 ( 5 )   4025 - 4034   2023.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d2cp05025k

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  • Nanospacial effect of citric acid-coordinated hydroxyapatite nanoparticle films on protein adsorption and cell adhesion states

    Zizhen Liu, Daisuke Kawagoe, Motohiro Tagaya

    JOURNAL OF MATERIALS CHEMISTRY B   10 ( 46 )   9599 - 9606   2022.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d2tb01240e

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  • Surface modification of hydroxyapatite nanoparticles for bone regeneration by controlling their surface hydration and protein adsorption states

    Zizhen Liu, Shota Yamada, Yuichi Otsuka, Tania Guadalupe Penaflor Galindo, Motohiro Tagaya

    DALTON TRANSACTIONS   51 ( 25 )   9572 - 9583   2022.6

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  • Investigation of Inclusion States of Silicate and Carbonate Ions in Hydroxyapatite Particles Prepared under the Presence of Sodium Silicate

    Tania Guadalupe Penaflor Galindo, Kazuto Sugimoto, Shota Yamada, Taito Sugibuchi, Zizhen Liu, Motohiro Tagaya

    BIOMIMETICS   7 ( 2 )   2022.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/biomimetics7020040

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  • Coordination State Control of Citric Acid Molecules on Europium(III) Ion-Doped Hydroxyapatite Nanoparticles for Highly Efficient Photoluminescence toward Biomedical Applications

    Takuya Kataoka, Takumi Hashimoto, Sadaki Samitsu, Zizhen Liu, Motohiro Tagaya

    ACS APPLIED NANO MATERIALS   5 ( 2 )   1 - 11   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsanm.1c03999

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  • Nanostructural control of transparent hydroxyapatite nanoparticle films using a citric acid coordination technique

    Zizhen Liu, Takuya Kataoka, Sadaki Samitsu, Daisuke Kawagoe, Motohiro Tagaya

    JOURNAL OF MATERIALS CHEMISTRY B   10 ( 3 )   396 - 405   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d1tb02002a

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  • Competitive incorporation of Eu(III) and Na(I) ions into citric acid-passivated hydroxyapatite particles

    Wanyu Shi, Takuya Kataoka, Takumi Hashimoto, Zizhen Liu, Motohiro Tagaya

    MATERIALS LETTERS   304   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.matlet.2021.130561

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  • Rubbing-assisted approach for highly-oriented collagen fibril arrays involving calcium phosphate precipitation

    Yadong Chai, Mitsuhiro Okuda, Mari Miyata, Zizhen Liu, Motohiro Tagaya

    MATERIALS CHEMISTRY FRONTIERS   5 ( 10 )   3936 - 3948   2021.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d1qm00114k

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  • Design of oriented mesoporous silica films for guiding protein adsorption states

    Yadong Chai, Yuri Maruko, Zizhen Liu, Motohiro Tagaya

    JOURNAL OF MATERIALS CHEMISTRY B   9 ( 8 )   2054 - 2065   2021.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d0tb02544e

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  • Preparation of transparent hydroxyapatite/citric acid particle films

    Zizhen Liu, Takuya Kataoka, Daisuke Kawagoe, Daichi Noda, Yadong Chai, Motohiro Tagaya

    COLLOID AND INTERFACE SCIENCE COMMUNICATIONS   39   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.colcom.2020.100316

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MISC

  • Biological Surface Layer Formation on Bioceramic Particles for Protein Adsorption

    Reo Kimura, Daichi Noda, Zizhen Liu, Wanyu Shi, Ryota Akutsu, Motohiro Tagaya

    BIOMIMETICS   9 ( 6 )   2024.6

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    Language：English   Publishing type：Book review, literature introduction, etc.  

    DOI： 10.3390/biomimetics9060347

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Presentations

  • Nanospacial Design of Citric Acid-Mediated Hydroxyapatite Nanoparticle Film for Improving Cell Adhesion

    Zizhen Liu, Motohiro Tagaya

    The 63rd Symposium on Basic Science of Ceramics  2025.1 

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  • クエン酸を介したアパタイトナノ粒子の自己集合構造 の形成と評価

    劉自振, 川越大輔, 多賀谷基博

    日本セラミックス協会 東北北海道支部 研究 発表会  2023.11 

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  • Investigation into Nanospacial Effect of Citric AcidCoordinated Hydroxyapatite Nanoparticle Films on Osteoblast Culture Ability.

    Z. Liu, K. Mikami, D. Kawagoe, M. Tagaya

    32nd Annual Meeting of MRS-Japan 2022,  2022.12 

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  • 生体液安定性を有するアパタイト／ヒアルロン酸ナノハイブリ ッド多孔質膜の合成

    遠藤 碧, 劉 自振, 多賀谷 基博

    日本セラミックス協会 第38回秋季シンポジ ウム  2025.9 

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  • Nanospace design in hydroxyapatite nanoparticle films for improving initial cell culture properties

    Zizhen Liu, Motohiro Tagaya

    6th International Conference on Nanospace Materials  2025.8 

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Industrial property rights

  • ハイブリッド粒子分散体及びその製造方法

    多賀谷 基博, 三上 久理守, 劉 自振

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    Applicant：国立大学法人長岡技術科学大学

    Application no：特願2024-031494  Date applied：2024.3

    Announcement no：特開2025-133500  Date announced：2025.9

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Awards

  • 笹川科学研究奨励賞

    2023.4   日本科学協会  

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  • 特定セッション学生優秀発表賞

    2022.9   日本セラミックス協会  

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