Updated on 2026/04/25

写真a

 
ASAI TAKEHIRO
 
Organization
School of Life Science and Technology Researcher
Title
Researcher
External link

Degree

  • Ph. D. (Agriculture) ( 2023.3   The University of Tokyo )

  • Master (Agriculture) ( 2019.3   The University of Tokyo )

  • Bachelor (Agriculture) ( 2017.3   The University of Tokyo )

Research Interests

  • 動物行動学

  • 行動神経科学

  • Non-AUG translation

  • 翻訳

Research Areas

  • Life Science / Applied microbiology

  • Life Science / Laboratory animal science

  • Life Science / Molecular biology

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

  • Life Science / Animal life science

  • Life Science / Neuroscience-general

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Education

  • The University of Tokyo   Graduate School of Agricultural and Life Sciences   Animal Resource Sciences

    2019.4 - 2023.3

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  • The University of Tokyo   Graduate School of Agricultural and Life Sciences   Biotechnology

    2017.4 - 2019.3

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  • The University of Tokyo

    2014.9 - 2017.3

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Research History

  • Institute of Science Tokyo   Researcher

    2024.10

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    Country：Japan

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  • The University of Tokyo   Graduate School of Agricultural and Life Sciences Veterinary Medical Sciences

    2023.5 - 2025.6

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  • The University of Tokyo   Graduate School of Agricultural and Life Sciences Veterinary Medical Sciences

    2023.4

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Professional Memberships

Papers

  • Loss of non-canonical translation initiation factors impairs perinatal cardiac function in mice.

    Takehiro Asai, Ryota Tochinai, Yoshiharu Tsuru, Marie Sekiguchi, Atsushi Minami, Wataru Fujii, Shigeru Kyuwa, Tetsuhiro Ogawa, Shigeru Kakuta

    Experimental animals   74 ( 4 )   429 - 439   2025.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    Translation regulation is crucial for cellular homeostasis. Recent studies have demonstrated that, in addition to the conventional AUG start codon, eukaryotic mRNA can also possess non-canonical start codons. These non-canonical start codons, including non-AUG codons, can be found both upstream and downstream of the conventional AUG start codon. Translation of these non-canonical open reading frames (ORFs) has been implicated in the development of diseases, such as cardiac diseases, neurodegeneration and cancer development. Non-AUG translation initiation is regulated by eukaryotic initiation factor (eIF) 2A and eIF2D; however, their target non-canonical ORFs, roles in disease development, and the underlying precise mechanisms of translation regulation remain poorly understood. To address these gaps, we generated mice lacking either or both of Eif2a and Eif2d genes on an ICR background and investigated their cardiac function using echocardiography. The results indicated that simultaneous disruption of both Eif2a and Eif2d led to perinatal cardiac impairment, as evidenced by a significant reduction in cardiac contractility as measured by ejection fraction. Furthermore, the absence of phenotypic changes in single knockouts of either Eif2a or Eif2d suggests that eIF2A and eIF2D function redundantly in their molecular roles. These findings underscore the importance of non-AUG translation initiation in maintaining cardiac function and suggest its broader implications in other physiological and pathological processes. We propose the Eif2a and Eif2d double-knockout mice as a novel stress-sensitive animal model to investigate the molecular mechanisms of translation regulation and their contribution to disease pathogenesis.

    DOI： 10.1538/expanim.25-0021

    PubMed

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  • Establishment of the improved colonization of Escherichia coli laboratory strain in the intestine mediated by single gene deletion

    Atsushi Minami, Takehiro Asai, Tsuyoshi Tachibana, Yu Tanaka, Mitsuyuki Nakajima, Shiori Tamura, Morihiro Nakazawa, Yoshiharu Tsuru, Yoichi Fujiyama, Yoh-ichi Tagawa, Tomohisa Kuzuyama, Shigeru Kakuta, Tetsuhiro Ogawa

    Biochemical and Biophysical Research Communications   2024.11

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2024.150448

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  • cAMP-CRP acts as a key regulator for the viable but non-culturable state in Escherichia coli. International journal

    Kazuki Nosho, Hiroko Fukushima, Takehiro Asai, Masahiro Nishio, Reiko Takamaru, Koseki Joseph Kobayashi-Kirschvink, Tetsuhiro Ogawa, Makoto Hidaka, Haruhiko Masaki

    Microbiology (Reading, England)   164 ( 3 )   410 - 419   2018.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    A variety of bacteria, including Escherichia coli, are known to enter the viable but non-culturable (VBNC) state under various stress conditions. During this state, cells lose colony-forming activities on conventional agar plates while retaining signs of viability. Diverse environmental stresses including starvation induce the VBNC state. However, little is known about the genetic mechanism inducing this state. Here, we aimed to reveal the genetic determinants of the VBNC state of E. coli. We hypothesized that the VBNC state is a process wherein specific gene products important for colony formation are depleted during the extended period of stress conditions. If so, higher expression of these genes would maintain colony-forming activities, thereby restraining cells from entering the VBNC state. From an E. coli plasmid-encoded ORF library, we identified genes that were responsible for maintaining high colony-forming activities after exposure to starvation condition. Among these, cpdA encoding cAMP phosphodiesterase exhibited higher performance in the maintenance of colony-forming activities. As cpdA overexpression decreases intracellular cAMP, cAMP or its complex with cAMP-receptor protein (CRP) may negatively regulate colony-forming activities under stress conditions. We confirmed this using deletion mutants lacking adenylate cyclase or CRP. These mutants fully maintained colony-forming activities even after a long period of starvation, while wild-type cells lost most of this activity. Thus, we concluded that the lack of cAMP-CRP effectively retains high colony-forming activities, indicating that cAMP-CRP acts as a positive regulator necessary for the induction of the VBNC state in E. coli.

    DOI： 10.1099/mic.0.000618

    PubMed

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Books

MISC

  • 非古典的な翻訳開始機構は周産期における母体循環器系機能の維持に寄与する

    浅井健宏, 浅井健宏, 南篤, 水流功春, 関口茉莉恵, 島野翔太郎, 栃内亮太, 富安博隆, チェンバーズ ジェームズ, 内田和幸, 藤井渉, 藤井渉, 葛山智久, 葛山智久, 久和茂, 久和茂, 小川哲弘, 小川哲弘, 角田茂, 角田茂, 角田茂

    日本実験動物学会総会講演要旨集(Web)   71st   2024

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  • マウスを用いた定着性改変大腸菌の腸管内分布の解析

    橘毅, 田中優, 中島満幸, 浅井健宏, 南篤, 田川陽一, 水流功春, 藤井渉, 藤井渉, 久和茂, 久和茂, 葛山智久, 葛山智久, 小川哲弘, 小川哲弘, 角田茂, 角田茂, 角田茂

    日本実験動物学会総会講演要旨集(Web)   71st   2024

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  • 非古典的翻訳開始因子の遺伝子変異マウスは周産期において循環器系機能に異常を示す

    浅井健宏, 南篤, 水流功春, 島野翔太朗, 関口茉莉恵, 橘毅, 栃内亮太, 栃内亮太, 富安博隆, チェンバーズ ジェームズ, 内田和幸, 藤井渉, 藤井渉, 葛山智久, 葛山智久, 久和茂, 久和茂, 久和茂, 小川哲弘, 小川哲弘, 角田茂, 角田茂, 角田茂

    日本実験動物学会総会講演要旨集(Web)   70th   2023

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  • Cardiac function during perinatal phase in non-canonical translation initiation factors mutant mice(タイトル和訳中)

    Asai Takehiro, Minami Atsushi, Tsuru Yoshi-haru, Shimano Shotaro, Sekiguchi Marie, Tachibana Tsuyoshi, Tochinai Ryota, Tomiyasu Hirotaka, Chambers James, Uchida Kazuyuki, Fujii Wataru, Kuzuyama Tomohisa, Kyuwa Shigeru, Ogawa Tetsuhiro, Kakuta Shigeru

    Experimental Animals   72 ( Suppl. )   S103 - S103   2023

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    Language：English   Publisher：(公社)日本実験動物学会  

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  • 非古典的翻訳開始機構不全に起因するストレス適応障害を対象とした疾患モデルマウスの開発

    浅井 健宏, 南 篤, 栃内 亮太, 水流 功春, 関口 茉莉恵, 藤井 渉, 葛山 智久, 久和 茂, 小川 哲弘, 角田 茂

    日本獣医学会学術集会講演要旨集   165回   [K2P - 13]   2022.9

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    Language：Japanese   Publisher：(公社)日本獣医学会  

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  • 高バイオフィルム形成大腸菌のマウス腸管内定着性コントロール

    中島満幸, 浅井健宏, 南篤, 水流功春, 田川陽一, 葛山智久, 久和茂, 小川哲弘, 角田茂

    日本実験動物学会総会講演要旨集(Web)   69th   2022

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Presentations

  • 遺伝子欠損マウスを用いたオートファジー進行過程に果たすRNase T2の機能の解析

    浅井 健宏, 王 辰, 藤井 渉, チェンバーズ ジェームズ, 内田 和幸, 村山 正承, 小川 修平, 小川 道永, 久和 茂, 岩倉 洋一郎, 小川 哲弘, 角田 茂

    第167回日本獣医学会学術集会  2024.9 

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    Event date： 2024.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：帯広   Country：Japan  

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  • マウスを用いた定着性改変大腸菌の腸管内分布の解析

    橘毅, 田中優, 中島満幸, 浅井健宏, 南篤, 田川陽一, 水流功春, 藤井渉, 久和茂, 葛山智久, 小川哲弘, 角田茂

    第71回日本実験動物学会総会  2024.5 

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    Event date： 2024.5

    Language：Japanese   Presentation type：Poster presentation  

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  • 非古典的な翻訳開始機構は周産期における母体循環器系機能の維持に寄与する

    浅井健宏, 南篤, 水流功春, 関口茉莉恵, 島野翔太郎, 栃内亮太, 富安博隆, チェンバーズ ジェームズ, 内田和幸, 藤井渉, 葛山智久, 久和茂, 小川哲弘, 角田茂

    第71回日本実験動物学会総会  2024.5 

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    Event date： 2024.5

    Language：Japanese   Presentation type：Poster presentation  

    Venue：京都   Country：Japan  

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  • Cardiac function during perinatal phase in non-canonical translation initiation factors mutant mice

    Takehiro Asai, Atsushi Minami, Yoshi-haru Tusru, Shotaro Shimano, Marie Sekiguchi, Tsuyoshi Tachibana, Ryota Tochinai, Hirotaka Tomiyasu, James K. Chambers, Kazuyuki Uchida, Wataru Fujii, Tomohisa Kuzuyama, Shigeru Kyuwa, Tetsuhiro Ogawa, Shigeru Kakuta

    The 70th Annual Meeting of the Japanese Association for Laboratory Animal Science  2023.5 

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    Event date： 2023.5

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 非古典的翻訳開始機構不全に起因するストレス適応障害を対象とした疾患モデルマウスの開発

    浅井健宏, 南 篤, 栃内 亮太, 水流 功春, 関口茉莉恵, 藤井渉, 葛山智久, 久和茂, 小川哲弘, 角田茂

    第165回日本獣医学会学術集会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 高バイオフィルム形成大腸菌のマウス腸管内定着性コントロール

    中島満幸, 浅井健宏, 南篤, 水流功春, 田川陽一, 葛山智久, 久和茂, 小川哲弘, 角田茂

    第69回日本実験動物学会（仙台）  2022.5 

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    Event date： 2022.5

    Language：Japanese   Presentation type：Poster presentation  

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  • In vivo evaluation of E. coli mutants with highly colonization ability

    2021.5 

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    Event date： 2021.5

    Presentation type：Oral presentation (general)  

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Awards

  • 2025 Experimental Animals Best Paper Award

    2026   Japanese Association for Laboratory Animal Science   Loss of non-canonical translation initiation factors impairs perinatal cardiac function in mice

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    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

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Teaching Experience

  • 毒性学実習（実験動物担当分）

    2021.10 - 2024.10 Institution：東京大学農学部獣医学専修

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  • 生体機能学実習（実験動物担当分）

    2021.4 - 2025.4 Institution：東京大学農学部獣医学専修

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  • 実験動物学実習

    2021.1 - 2025.3 Institution：東京大学農学部獣医学専修

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  • 実験動物学実習講義

    2021.1 - 2022.1 Institution：東京大学農学部獣医学専修

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