Updated on 2026/04/29

写真a

 
TERASAKA NAOHIRO
 
Organization
Institute of Future Science Earth-Life Science Institute Specially Appointed Associate Professor
Title
Specially Appointed Associate Professor
Homepage
https://nterasaka.wixsite.com/terasaka-lab/en
External link

Degree

  • 博士（理学） ( 東京大学 )

Research Interests

  • カプシドタンパク質

  • tRNA

  • リボソーム

  • 遺伝暗号

  • ペプチド

  • リボザイム

  • RNA

  • ケミカルバイオロジー

  • 分子進化工学

  • 合成生物学

Research Areas

  • Life Science / Evolutionary biology

  • Nanotechnology/Materials / Bio chemistry

Education

  • The University of Tokyo   Graduate School of Science   Department of Chemistry

    2012.4 - 2015.3

    　 More details

  • The University of Tokyo   The Graduate School of Engineering   Department of Chemistry and Biotechnology

    2010.4 - 2012.3

    　 More details

  • The University of Tokyo   The Faculty of Engineering   Department of Chemistry and Biotechnology

    2008.4 - 2010.3

    　 More details

  • The University of Tokyo   College of Arts and Sciences

    2006.4 - 2008.3

    　 More details

  • 駒場東邦高等学校

    2003.4 - 2006.3

    　 More details

Research History

  • 国立研究開発法人 科学技術振興機構 創発的研究支援事業研究者

    2025.4

    　 More details

  • Institute of Science Tokyo   Earth-Life Science Institute, Institute of Future Science   Specially Appointed Associate Professor

    2024.10

    　 More details

  • Tokyo Institute of Technology   International Research Frontiers Initiative, Earth-Life Science Institute   Specially Appointed Associated Professor

    2022.6 - 2024.9

    　 More details

    Country：Japan

    researchmap

  • The University of Tokyo   Graduate School of Science Department of Chemistry   Assistant Professor

    2022.4 - 2022.6

    　 More details

  • 国立研究開発法人 科学技術振興機構 さきがけ研究 「細胞の動的高次構造体」 研究者

    2021.10 - 2025.3

    　 More details

  • The University of Tokyo   Graduate School of Science, Department of Chemistry   Project Assistant Professor

    2018.6 - 2022.3

    　 More details

  • ETH Zurich   Postdoctoral Associate

    2015.4 - 2018.5

    　 More details

  • ヒューマン・フロンティア・サイエンス・プログラム (HFSP) 長期フェロー

    2015.4 - 2018.3

    　 More details

  • 日本学術振興会 特別研究員（DC1）

    2012.4 - 2015.3

    　 More details

▼display all

Professional Memberships

▼display all

Papers

  • In Vitro Evolution of the Adenosine A2A Receptor Based on an Antagonist Binding Using a Ribosome Display Reviewed

    Genki Fukasawa, Yuma Matsuoka, Duy Phuoc Tran, Haruka Nishigaki, Keisuke Fukunaga, Takayoshi Watanabe, Tomohiro Doura, Naohiro Terasaka, Ako Kagawa, Takeshi Murata, Akio Kitao, Shigeki Kiyonaka, Tomoaki Matsuura

    Journal of the American Chemical Society   2026.3

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/jacs.6c02372

    researchmap

  • Efficient cell-free evolution of RNA polymerases by droplet microfluidics

    Naohiro Terasaka, Taro Furubayashi, Kenya Tajima, Hiroyuki Noji

    bioRxiv   2026.2

    　More details

    Authorship：Lead author,　Corresponding author  

    DOI： 10.64898/2026.02.23.707346

    researchmap

  • Cargo-Directed Assembly of Nonviral Nucleocapsid with Controlled Size

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    ACS Synthetic Biology   2026.2

    　More details

    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Cold Spring Harbor Laboratory  

    Precise packaging of diverse cargo within self-assembling protein cages of defined size and shape is essential for many biotechnological applications, yet cellular expression offers limited control over loading. Here, we developed a system for in vitro cargo-directed reconstitution of a split, laboratory evolved nonviral nucleocapsid (spNC-4). Independently expressed and purified spNC-4 capsid protein subunits were mixed and assembled with cargo molecules in a cooperative manner. As an authentic cargo, mRNA is packaged into a 30 nm-spheric nucleocapsid in vitro, closely matching to spNC-4 expressed in cells. In this system, a diverse range of cargo molecules, including cognate nucleocapsid mRNA, noncognate RNA, RNA complexed with positively supercharged fluorescent protein, and linear double-stranded DNA are encapsulated within the 30 nm-spheric nucleocapsids in vitro. Moreover, the packaging of 30 nm-spherical or rod-shaped DNA origamis as templates induce morphological alterations of the nucleocapsids, resulting in the formation of enlarged 60 nm-spherical structure or rod-shaped structure, respectively. This split-protein, cargo-dependent system provides versatile and programmable control over both composition and architecture of nonviral protein cages, creating a general platform for enzyme nanoreactors, targeted delivery, and vaccine development.

    DOI： 10.1021/acssynbio.5c00881

    researchmap

  • Boosted cell-free gene expression for robust signal readout from a single-copy DNA template in microdroplets

    Taro Furubayashi, Naohiro Terasaka, Kenya Tajima, Hiroyuki Noji

    bioRxiv   2026.2

    　More details

  • A Nonviral Neo‐Nucleocapsid for Cell‐Specific RNA Delivery Developed by Pseudo‐Cyclic Peptide Grafting and Directed Evolution Reviewed

    Daiki Kanayama, Naohiro Terasaka, Kazuki Kato, Sae Okazaki, Hiroaki Suga

    Angewandte Chemie International Edition   2026.2

    　More details

    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/anie.202519027

    researchmap

  • An engineered closed-shell, two-component, 480-subunit nucleocapsid

    Mikail D. Levasseur, Naohiro Terasaka, Angela Steinauer, Stephan Tetter, Sara Pfister, Beat H. Meier, Donald Hilvert

    bioRxiv   2025.10

    　More details

    Authorship：Lead author  

    DOI： 10.1101/2025.10.22.683813

    researchmap

  • Optimization of Malonyl Coenzyme A Biosensors in a Reconstituted Cell-Free System for Detecting Acetyl-CoA Carboxylase Activity Reviewed

    Shohei Ito, Shota Nishikawa, Naohiro Terasaka, Kosuke Fujishima

    ACS Synthetic Biology   14 ( 8 )   3245 - 3251   2025.7

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acssynbio.5c00361

    PubMed

    researchmap

  • De Novo Peptides That Induce the Liquid–Liquid Phase Separation of α-Synuclein Reviewed

    Tatsuya Ikenoue, Masatomo So, Naohiro Terasaka, Wei-En Huang, Yasushi Kawata, Yohei Miyanoiri, Kiyoto Kamagata, Hiroaki Suga

    Journal of the American Chemical Society   147 ( 27 )   24113 - 24126   2025.6

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/jacs.5c08019

    researchmap

  • A versatile method of ribosomal synthesis of peptides containing long chain fatty acids at the N-terminus Reviewed

    Hiroki Murakami, Naohiro Terasaka, Haruo Aikawa, Hiroaki Suga

    Bulletin of the Chemical Society of Japan   98 ( 4 )   2025.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    The N-terminal modification of peptides and proteins with long chain fatty acids (LCFAs) plays an important role in their cellular bioactivity and localization. Such modifications are generally carried out by specific enzymes in a post-translation manner, and therefore an alteration of the N-terminal modifying groups requires either engineering the enzymes or their unusual intrinsic promiscuity. Here we report a versatile method using an approach of in vitro ribosomal synthesis of peptides containing various long fatty acids at the N-terminus. We charged Gly on an initiator tRNA by flexizyme, and then the free N-amino group was chemically acylated with myristoyl chloride and applied to the ribosomal synthesis of a myristoyl-peptide according to the designated mRNA template sequence. This approach turns out to be applicable for a wide array of not only initiator amino acids but also LCFAs. To this end, we have demonstrated the ribosomal synthesis of the Myrcludex B, an inhibitor peptide of hepatitis B virus infection containing the myristoyl group, and its variants containing other LCFA groups.

    DOI： 10.1093/bulcsj/uoaf027

    researchmap

    Other Link： https://academic.oup.com/bcsj/article-pdf/98/4/uoaf027/62775731/uoaf027.pdf

  • Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System Reviewed

    Jinxuan Zhao, Yi Li, Naohiro Terasaka, Haruo Aikawa, Hiroaki Suga

    ACS Central Science   11 ( 3 )   431 - 440   2025.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acscentsci.4c01021

    researchmap

  • RaPID discovery of cell-permeable helical peptide inhibitors con-taining cyclic β-amino acids against SARS-CoV-2 main protease Reviewed

    Marina Kawai, Tika R. Malla, H. T. Henry Chan, Anthony Tumber, Lennart Brewitz, Eidarus Salah, Naohiro Terasaka, Takayuki Katoh, Akane Kawamura, Christopher J. Schofield, Fernanda Duarte, Hiroaki Suga

    RSC Chemical Biology   6 ( 7 )   1089 - 1099   2025

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    The ribosomal synthesis of a helical peptide library containing structurally constrained cyclic β-amino acid at every third position and its application for the RaPID selection against SARS-CoV-2 main protease (Mpro) inhibitors.

    DOI： 10.1039/d5cb00021a

    researchmap

  • An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA in situ of a custom-made translation system for incorporation Reviewed

    Wei Lu, Naohiro Terasaka, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki, Hiroaki Suga

    Nucleic Acids Research   52 ( 7 )   3938 - 3949   2024.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    In the hypothetical RNA world, ribozymes could have acted as modern aminoacyl-tRNA synthetases (ARSs) to charge tRNAs, thus giving rise to the peptide synthesis along with the evolution of a primitive translation apparatus. We previously reported a T-boxzyme, Tx2.1, which selectively charges initiator tRNA with N-biotinyl-phenylalanine (BioPhe) in situ in a Flexible In-vitro Translation (FIT) system to produce BioPhe-initiating peptides. Here, we performed in vitro selection of elongation-capable T-boxzymes (elT-boxzymes), using para-azido-l-phenylalanine (PheAZ) as an acyl-donor. We implemented a new strategy to enrich elT-boxzyme-tRNA conjugates that self-aminoacylated on the 3′-terminus selectively. One of them, elT32, can charge PheAZ onto tRNA in trans in response to its cognate anticodon. Further evolution of elT32 resulted in elT49, with enhanced aminoacylation activity. We have demonstrated the translation of a PheAZ-containing peptide in an elT-boxzyme-integrated FIT system, revealing that elT-boxzymes are able to generate the PheAZ-tRNA in response to the cognate anticodon in situ of a custom-made translation system. This study, together with Tx2.1, illustrates a scenario where a series of ribozymes could have overseen aminoacylation and co-evolved with a primitive RNA-based translation system.

    DOI： 10.1093/nar/gkae151

    researchmap

  • Cyclic β2,3-amino acids improve the serum stability of macrocyclic peptide inhibitors targeting the SARS-CoV-2 main protease Reviewed

    Takashi Miura, Tika R Malla, Lennart Brewitz, Anthony Tumber, Eidarus Salah, Kang Ju Lee, Naohiro Terasaka, C David Owen, Claire Strain-Damerell, Petra Lukacik, Martin A Walsh, Akane Kawamura, Christopher J Schofield, Takayuki Katoh, Hiroaki Suga

    Bulletin of the Chemical Society of Japan   97 ( 5 )   2024.3

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Due to their constrained conformations, cyclic β2,3-amino acids (cβAA) are key building blocks that can fold peptides into compact and rigid structures, improving peptidase resistance and binding affinity to target proteins, due to their constrained conformations. Although the translation efficiency of cβAAs is generally low, our engineered tRNA, referred to as tRNAPro1E2, enabled efficient incorporation of cβAAs into peptide libraries using the flexible in vitro translation (FIT) system. Here we report on the design and application of a macrocyclic peptide library incorporating 3 kinds of cβAAs: (1R,2S)-2-aminocyclopentane carboxylic acid (β1), (1S,2S)-2-aminocyclohexane carboxylic acid (β2), and (1R,2R)-2-aminocyclopentane carboxylic acid. This library was applied to an in vitro selection against the SARS-CoV-2 main protease (Mpro). The resultant peptides, BM3 and BM7, bearing one β2 and two β1, exhibited potent inhibitory activities with IC50 values of 40 and 20 nM, respectively. BM3 and BM7 also showed remarkable serum stability with half-lives of 48 and >168 h, respectively. Notably, BM3A and BM7A, wherein the cβAAs were substituted with alanine, lost their inhibitory activities against Mpro and displayed substantially shorter serum half-lives. This observation underscores the significant contribution of cβAA to the activity and stability of peptides. Overall, our results highlight the potential of cβAA in generating potent and highly stable macrocyclic peptides with drug-like properties.

    DOI： 10.1093/bulcsj/uoae018

    researchmap

    Other Link： https://academic.oup.com/bcsj/article-pdf/97/5/uoae018/57922858/uoae018.pdf

  • De Novo Single-Stranded RNA-Binding Peptides Discovered by Codon-Restricted mRNA Display Reviewed

    Shota Nishikawa, Hidenori Watanabe, Naohiro Terasaka, Takayuki Katoh, Kosuke Fujishima

    Biomacromolecules   25 ( 1 )   355 - 365   2023.12

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.biomac.3c01024

    researchmap

  • MET‐Activating Ubiquitin Multimers Reviewed

    Naoya Kawakami, Hiroki Sato, Naohiro Terasaka, Kunio Matsumoto, Hiroaki Suga

    Angewandte Chemie International Edition   62 ( 36 )   2023.7

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Receptor tyrosine kinases (RTKs) are generally activated through their dimerization and/or oligomerization induced by their cognate ligands, and one such RTK hepatocyte growth factor (HGF) receptor, known as MET, plays an important role in tissue regeneration. Here we show the development of ubiquitin (Ub)‐based protein ligand multimers, referred to as U‐bodies, which act as surrogate agonists for MET and are derived from MET‐binding macrocyclic peptides. Monomeric Ub constructs (U‐body) were first generated by genetic implantation of a macrocyclic peptide pharmacophore into a structural loop of Ub (lasso‐grafting) and subsequent optimization of its flanking spacer sequences via mRNA display. Such U‐body constructs exhibit potent binding affinity to MET, thermal stability, and proteolytic stability. The U‐body constructs also partially/fully inhibited or enhanced HGF‐induced MET‐phosphorylation. Their multimerization to dimeric, tetrameric, and octameric U‐bodies linked by an appropriate peptide linker yielded potent MET activation activity and downstream cell proliferation‐promoting activity. This work suggests that lasso‐grafting of macrocycles to Ub is an effective approach to devising protein‐based artificial RTK agonists and it can be useful in the development of a new class of biologics for various therapeutic applications.

    DOI： 10.1002/anie.202307157

    researchmap

  • In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main protease Reviewed

    Takashi Miura, Tika R. Malla, C. David Owen, Anthony Tumber, Lennart Brewitz, Michael A. McDonough, Eidarus Salah, Naohiro Terasaka, Takayuki Katoh, Petra Lukacik, Claire Strain-Damerell, Halina Mikolajek, Martin A. Walsh, Akane Kawamura, Christopher J. Schofield, Hiroaki Suga

    Nature Chemistry   15 ( 7 )   998 - 1005   2023.5

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    γ-Amino acids can play important roles in the biological activities of natural products; however, the ribosomal incorporation of γ-amino acids into peptides is challenging. Here we report how a selection campaign employing a non-canonical peptide library containing cyclic γ2,4-amino acids resulted in the discovery of very potent inhibitors of the SARS-CoV-2 main protease (Mpro). Two kinds of cyclic γ2,4-amino acids, cis-3-aminocyclobutane carboxylic acid (γ1) and (1R,3S)-3-aminocyclopentane carboxylic acid (γ2), were ribosomally introduced into a library of thioether-macrocyclic peptides. One resultant potent Mpro inhibitor (half-maximal inhibitory concentration = 50 nM), GM4, comprising 13 residues with γ1 at the fourth position, manifests a 5.2 nM dissociation constant. An Mpro:GM4 complex crystal structure reveals the intact inhibitor spans the substrate binding cleft. The γ1 interacts with the S1′ catalytic subsite and contributes to a 12-fold increase in proteolytic stability compared to its alanine-substituted variant. Knowledge of interactions between GM4 and Mpro enabled production of a variant with a 5-fold increase in potency.

    DOI： 10.1038/s41557-023-01205-1

    researchmap

    Other Link： https://www.nature.com/articles/s41557-023-01205-1

  • De Novo Discovery of Thiopeptide Pseudo-natural Products Acting as Potent and Selective TNIK Kinase Inhibitors Reviewed

    Alexander A. Vinogradov, Yue Zhang, Keisuke Hamada, Jun Shi Chang, Chikako Okada, Hirotaka Nishimura, Naohiro Terasaka, Yuki Goto, Kazuhiro Ogata, Toru Sengoku, Hiroyasu Onaka, Hiroaki Suga

    Journal of the American Chemical Society   144 ( 44 )   20332 - 20341   2022.10

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/jacs.2c07937

    researchmap

  • Macrocyclic Peptide-Conjugated Tip for Fast and Selective Molecular Recognition Imaging by High-Speed Atomic Force Microscopy Reviewed

    Leonardo Puppulin, Daiki Kanayama, Naohiro Terasaka, Katsuya Sakai, Noriyuki Kodera, Kenichi Umeda, Ayumi Sumino, Arin Marchesi, Wei Weilin, Hideo Tanaka, Takeshi Fukuma, Hiroaki Suga, Kunio Matsumoto, Mikihiro Shibata

    ACS Applied Materials & Interfaces   13 ( 46 )   54817 - 54829   2021.11

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acsami.1c17708

    researchmap

  • De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist Reviewed

    Yamato Komatsu, Naohiro Terasaka, Katsuya Sakai, Emiko Mihara, Risa Wakabayashi, Kunio Matsumoto, Donald Hilvert, Junichi Takagi, Hiroaki Suga

    iScience   103302 - 103302   2021.10

    　More details

    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.isci.2021.103302

    researchmap

  • Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein Reviewed

    Stephan Tetter, Naohiro Terasaka, Angela Steinauer, Richard J. Bingham, Sam Clark, Andrew J. P. Scott, Nikesh Patel, Marc Leibundgut, Emma Wroblewski, Nenad Ban, Peter G. Stockley, Reidun Twarock, Donald Hilvert

    Science   372 ( 6547 )   1220 - 1224   2020.12

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：American Association for the Advancement of Science ({AAAS})  

    Viruses are ubiquitous pathogens of global impact. Prompted by the hypothesis that their earliest progenitors recruited host proteins for virion formation, we have used stringent laboratory evolution to convert a bacterial enzyme that lacks affinity for nucleic acids into an artificial nucleocapsid that efficiently packages and protects multiple copies of its own encoding messenger RNA. Revealing remarkable convergence on the molecular hallmarks of natural viruses, the accompanying changes reorganized the protein building blocks into an interlaced 240-subunit icosahedral capsid that is impermeable to nucleases, and emergence of a robust RNA stem-loop packaging cassette ensured high encapsidation yields and specificity. In addition to evincing a plausible evolutionary pathway for primordial viruses, these findings highlight practical strategies for developing nonviral carriers for diverse vaccine and delivery applications.

    DOI： 10.1101/2020.12.23.423990

    researchmap

  • Macrocyclic Peptide-Mediated Blockade of the CD47-SIRPα Interaction as a Potential Cancer Immunotherapy Reviewed

    Daisuke Hazama, Yizhen Yin, Yoji Murata, Makoto Matsuda, Takeshi Okamoto, Daisuke Tanaka, Naohiro Terasaka, Jinxuan Zhao, Mariko Sakamoto, Yuka Kakuchi, Yasuyuki Saito, Takenori Kotani, Yoshihiro Nishimura, Atsushi Nakagawa, Hiroaki Suga, Takashi Matozaki

    Cell Chemical Biology   27 ( 9 )   1181 - 1191.e7   2020.9

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.chembiol.2020.06.008

    researchmap

  • An aminoacylation ribozyme evolved from a natural tRNA-sensing T-box riboswitch Reviewed

    Satoshi Ishida, Naohiro Terasaka, Takayuki Katoh, Hiroaki Suga

    Nature Chemical Biology   16 ( 6 )   702 - 709   2020.3

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41589-020-0500-6

    researchmap

    Other Link： http://www.nature.com/articles/s41589-020-0500-6

  • Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies Reviewed

    Hanne L. P. Tytgat, Chia-wei Lin, Mikail D. Levasseur, Markus B. Tomek, Christoph Rutschmann, Jacqueline Mock, Nora Liebscher, Naohiro Terasaka, Yusuke Azuma, Michael Wetter, Martin F. Bachmann, Donald Hilvert, Markus Aebi, Timothy G. Keys

    Nature Communications   10 ( 1 )   2019.11

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Glycosylation of proteins profoundly impacts their physical and biological properties. Yet our ability to engineer novel glycoprotein structures remains limited. Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively. Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm. The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm. We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope. This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.

    DOI： 10.1038/s41467-019-13283-2

    researchmap

    Other Link： https://www.nature.com/articles/s41467-019-13283-2

  • Generation of non-standard macrocyclic peptides specifically binding TSC-22 homologous gene-1 Reviewed

    Sophie T. PhuongDung Tran, Christopher J. Hipolito, Hiroyuki Suzuki, Rudy Xie, Huynh Dam Kim Tuyen, Peter ten Dijke, Naohiro Terasaka, Yuki Goto, Hiroaki Suga, Mitsuyasu Kato

    Biochemical and Biophysical Research Communications   516 ( 2 )   445 - 450   2019.8

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bbrc.2019.06.035

    PubMed

    researchmap

  • Laboratory evolution of virus-like nucleocapsids from nonviral protein cages Reviewed

    Naohiro Terasaka, Yusuke Azuma, Donald Hilvert

    Proceedings of the National Academy of Sciences   115 ( 21 )   5432 - 5437   2018.5

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Proceedings of the National Academy of Sciences  

    DOI： 10.1073/pnas.1800527115

    PubMed

    researchmap

  • Modular Protein Cages for Size-Selective RNA Packaging in Vivo. Reviewed

    Azuma Y, Edwardson TGW, Terasaka N, Hilvert D

    Journal of the American Chemical Society   140 ( 2 )   566 - 569   2018.1

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/jacs.7b10798

    PubMed

    researchmap

  • A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX. Reviewed

    Terasaka N, Futai K, Katoh T, Suga H

    RNA   22 ( 12 )   1918 - 1928   2016.12

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1261/rna.057737.116

    PubMed

    researchmap

  • tRid, an enabling method to isolate previously inaccessible small RNA fractions. Reviewed

    Futai K, Terasaka N, Katoh T, Suga H

    Methods   106   105 - 111   2016.8

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ymeth.2016.04.033

    PubMed

    researchmap

  • Recent developments of engineered translational machineries for the incorporation of non-canonical amino acids into polypeptides. Reviewed

    Terasaka N, Iwane Y, Geiermann AS, Goto Y, Suga H

    International journal of molecular sciences   16 ( 3 )   6513 - 6531   2015.3

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms16036513

    PubMed

    researchmap

  • An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center. Reviewed

    Terasaka N, Hayashi G, Katoh T, Suga H

    Nature chemical biology   10 ( 7 )   555 - 557   2014.7

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nchembio.1549

    PubMed

    researchmap

  • Flexizymes-facilitated Genetic Code Reprogramming Leading to the Discovery of Drug-like Peptides Reviewed

    Naohiro Terasaka, Hiroaki Suga

    Chem. Lett.   43 ( 1 )   11 - 19   2014.1

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1246/cl.130910

    Web of Science

    researchmap

  • Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA(Ile2) and ATP. Reviewed

    Osawa T, Inanaga H, Kimura S, Terasaka N, Suzuki T, Numata T

    Acta crystallographica. Section F, Structural biology and crystallization communications   67 ( Pt 11 )   1414 - 1416   2011.11

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1107/S1744309111034890

    PubMed

    researchmap

  • Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS. Reviewed

    Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T

    Nature structural & molecular biology   18 ( 11 )   1268 - 1274   2011.11

    　More details

    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nsmb.2121

    PubMed

    researchmap

  • Structural basis of tRNA agmatinylation essential for AUA codon decoding. Reviewed

    Osawa T, Kimura S, Terasaka N, Inanaga H, Suzuki T, Numata T

    Nature structural & molecular biology   18 ( 11 )   1275 - 1280   2011.11

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nsmb.2144

    PubMed

    researchmap

  • The RNA origin of transfer RNA aminoacylation and beyond. Reviewed

    Suga H, Hayashi G, Terasaka N

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences   366 ( 1580 )   2959 - 2964   2011.10

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1098/rstb.2011.0137

    PubMed

    researchmap

▼display all

Books

  • 生体分子の実験的進化工学

    寺坂尚紘（ Role： Contributor）

    生体の科学 Vol.76 No.2 2025年 04月号 特集 AIにより加速する生体分子デザイン  2025.4 

    　More details

  • 特殊環状ペプチドライブラリの構築と薬剤候補探索

    川合茉利奈, 井上澄香, 寺坂尚紘, 菅裕明

    CSJ Current Review 生体分子と疾患ーヘルスサイエンスの切り札としての化学ー  2021.8 

    　More details

  • 試験管内分子進化法によるバイオマテリアル創成

    寺坂尚紘, 菅裕明（ Role： Joint author）

    日本核酸化学会 核酸科学ハンドブック  2020.12 

    　More details

    Responsible for pages：201  

    researchmap

  • Applications of aminoacylation ribozymes that recognize the 3’-end of tRNA

    寺坂 尚紘

    Springer  2017 

    　More details

  • 遺伝暗号のリプログラミングによる特殊ペプチドライブラリーの構築と生理活性ペプチド探索

    寺坂尚紘, 山岸祐介, 菅裕明

    実験医学増刊号 Vol.29 No.7, 75-82  2011 

    　More details

MISC

  • 実験室内進化で迫る原始ウイルス形成過程 Invited Reviewed

    寺坂尚紘

    Viva Origino   52 ( 1 )   2024.5

    　More details

  • 細菌タンパクを用いたウイルス様構造とパッケージング機構の進化

    寺坂 尚紘, Donald Hilver

    Science に載った日本人研究者 2021   34   2022

    　More details

  • 細菌タンパク質をウイルス模倣ヌクレオカプシドに分子進化させる

    寺坂尚紘

    生化学   94 ( 5 )   2022

    　More details

  • リボソーム翻訳系と修飾酵素を用いた非リボソームペプチド類似体の合成

    寺坂 尚紘

    ファルマシア   57 ( 6 )   543 - 543   2021

    　More details

    Language：Japanese   Publisher：公益社団法人 日本薬学会  

    複数の抗生物質に耐性を示す多剤耐性菌による感染症は世界中で問題になっている．さらに，認可される抗生物質は過去数十年で減少しており，新たな抗生物質探索方法の開発は急務である．ペプチド性天然物である非リボソームペプチド(NRPs)は多彩な構造を有し，生理活性が期待できる．例えばバンコマイシンやダプトマイシンなどの抗菌NRPsが発見されており，NRPs類似化合物のライブラリーは，新規抗生物質の探索に有用であると考えられる．しかし，NRPs合成酵素は高い基質選択性を持つ巨大複合体であるため，酵素改変による多様性の高いNRPsライブラリー構築は未だ困難である．<br>一方で，リボソーム翻訳系翻訳後修飾ペプチド(RiPPs)は，DNAから転写・翻訳されたペプチドに翻訳後修飾酵素が化学修飾を導入することで合成される．RiPPsの翻訳後修飾酵素の多くは基質許容性が高く，さらに縮重塩基を持つDNAを用いることで，高多様性ペプチドライブラリーの構築が可能である．本稿では，ハイスループットスクリーニング系に適用可能なRiPPs合成系を用いて，抗菌活性を持つNRPs類似化合物を合成するアプローチに関する論文を紹介する．<br>なお，本稿は下記の文献に基づいて，その研究成果を紹介するものである.<br>1) Niquille D. L. et al., Nat. Chem., 10, 282-287(2018)．<br>2) Zhao X. et al., Cell. Chem. Biol., 27, 1262-1271(2020)．<br>3) Li Y. X. et al., Nat. Commun., 9, 3273(2018)．<br>4) Urban J. H. et al., Nat. Commun., 8, 1500(2017)．

    DOI： 10.14894/faruawpsj.57.6_543

    CiNii Research

    researchmap

Presentations

  • マイクロ流路デバイスを用いた 無細胞タンパク質スクリーニング

    寺坂尚紘

    日本薬学会第146年会  2026.3 

    　More details

    Event date： 2026.3

    Presentation type：Oral presentation (general)  

    researchmap

  • Laboratory evolution of virus-like ribonucleoprotein complexes Invited

    International Symposium of Life-Nonlife Transition Boundaries Between Life and Nonlife  2026.3 

    　More details

    Event date： 2026.3

    Language：English   Presentation type：Oral presentation (invited, special)  

    researchmap

  • 油中水滴を用いた無細胞酵素進化システム

    寺坂尚紘

    日本農芸化学会2026年度京都大会  2026.3 

    　More details

    Event date： 2026.3

    Presentation type：Oral presentation (general)  

    researchmap

  • Directed Evolution of RNA-Encapsulating Protein Nucleocapsids with Distinct Sizes

    Hualin Li, Tomoyuki Numata, Tomoaki Matsuura, Naohiro Terasaka

    14th ELSI International Symposium  2026.1 

    　More details

    Event date： 2026.1

    researchmap

  • Innovative Lactate Indicators Developed Using High-Throughput Techniques

    Zhengyang Luo, Yusuke Nasu, Tomoaki Matsuura, Naohiro Terasaka

    14th ELSI International Symposium  2026.1 

    　More details

    Event date： 2026.1

    researchmap

  • Laboratory evolution of membrane associated peptides

    Cao Wenke, Tomoaki Matsuura, Naohiro Terasaka

    14th ELSI International Symposium  2026.1 

    　More details

    Event date： 2026.1

    researchmap

  • Functionalization and size control of laboratory evolved artificial nucleocapsids

    2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Oral presentation (general)  

    researchmap

  • Cell-free evolution of fluorescent protein-based lactate biosensors

    Zhengyang Luo, Yusuke Nasu, Naohiro Terasaka

    2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Poster presentation  

    researchmap

  • Directed Evolution of Nonviral Nucleocapsids with Enlarged Structures

    Hualin LI, Naohiro Terasaka

    2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Oral presentation (general)  

    researchmap

  • Cell-free evolution of anti-microbial peptide using microfluidics

    Wenke Cao, Naohiro Terasaka

    2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Oral presentation (general)  

    researchmap

  • 再構成型無細胞タンパク質合成系を用いたマロニルCoAバイオセンサーの最適化とアセチルCoAカルボキシラーゼ活性の検出

    伊藤 昇平, 西川 将太, 寺坂 尚紘, 藤島 皓介

    第48回日本分子生物学会年会  2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Poster presentation  

    researchmap

  • Structural and biochemical analyses of T-box riboswitch-based ribozyme catalyzing tRNA aminoacylation

    Takeru Kagawa, Kouhei Abe, Naohiro Terasaka, Hiroaki Suga, Tomoyuki Numata

    The 19th Conference of Asian Crystallographic Association 2025  2025.12 

    　More details

    Event date： 2025.12

    Presentation type：Poster presentation  

    researchmap

  • In vitro assembly of a nonviral protein capsid and its morphological control mediated by versatile cargos

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    The 63rd Annual Meeting of the Biophysical Society of Japan  2025.9 

    　More details

    Event date： 2025.9

    Presentation type：Poster presentation  

    researchmap

  • Functionalization and structural diversification of artificial nucleocapsids by laboratory evolution

    The 63rd Annual Meeting of the Biophysical Society of Japan  2025.9 

    　More details

    Event date： 2025.9

    Presentation type：Poster presentation  

    researchmap

  • ウイルス模倣ヌクレオカプシドの構造多様化と機能化

    寺坂尚紘

    「細胞を創る」研究会18.0  2025.9 

    　More details

    Event date： 2025.9

    Presentation type：Poster presentation  

    researchmap

  • 無細胞翻訳バイオセンサーとエマルジョン液滴を用いたCO2固定酵素の指向性進化

    伊藤昇平, 西川将太, 寺坂尚紘, 藤島皓介

    「細胞を創る」研究会18.0  2025.9 

    　More details

    Event date： 2025.9

    Presentation type：Poster presentation  

    researchmap

  • A HIGH-ACCURACY PAIR MATCHING METHOD FOR DROPLET MICROFLUIDICS WITH TWO-STATE MEASUREMENT POINTS

    Akihiro Isozaki, Yusuke Nasu, Naohiro Terasaka

    IEEE MEMS 2025  2025.1 

    　More details

    Event date： 2025.1

    researchmap

  • Structural expansion of nonviral nucleocapsids by laboratory evolution

    Hualin Li, Naohiro Terasaka

    13th ELSI International Symposium 2025  2025.1 

    　More details

    Event date： 2025.1

    researchmap

  • Directed evolution of CO2 fixation enzyme using cell free expression biosensor and droplet sorting

    Shohei Ito, Naohiro Terasaka, Shota Nishikawa, Kosuke Fujishima

    The 47th Annual Meeting of the Molecular Biology Society of Japan  2024.11 

    　More details

    Event date： 2024.11

    researchmap

  • Establishment of a platform for the development of novel xCT inhibitors

    Ryosuke Zaitsu, Yuto Ohno, Fumiya K Sano, Tatsuki Tanaka, Takaaki A Kobayashi, Yoshiaki Kise, Tsukasa Kusakizako, Haruo Aikawa, Naohiro Terasaka, Kazumasa Oda, Shojiro Kitajima, Hiroaki Suga, Osamu Nureki

    The 47th Annual Meeting of the Molecular Biology Society of Japan  2024.11 

    　More details

    Event date： 2024.11

    researchmap

  • A Simple peptide tag inducing protein condensates in cell-free translation system

    Naohiro Terasaka

    4th Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS2024)  2024.11 

    　More details

    Event date： 2024.11

    Language：English  

    researchmap

  • How does library size affect the biding kinetics of hits in RaPID selection?

    Haruo Aikawa, Jinxuan Zhao, Yi Li, Naohiro Terasaka, Hiroaki Suga

    4th Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS2024)  2024.11 

    　More details

    Event date： 2024.11

    researchmap

  • 進化工学による機能性分子の創出と生命起源研究への展開 Invited

    寺坂尚紘

    第11回 バイオ関連化学シンポジウム 若手フォーラム  2024.9 

    　More details

    Event date： 2024.9

    researchmap

  • Tandem artificial nucleocapsid to package longer RNA genome and expand protein architectures

    Hualin Li, Naohiro Terasaka

    21st IUPAB Congress 2024  2024.6 

    　More details

    Event date： 2024.6

    researchmap

  • In vitro assembly of protein capsule and cargo molecules into a virus-like particle

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    21st IUPAB Congress 2024  2024.6 

    　More details

    Event date： 2024.6

    researchmap

  • In vitro packaging of various cargo molecules into a non-viral protein cage

    Kenya Tajima, Yusuke Sakai, Naohiro Terasaka

    The 24th Annual Meeting of the Protein Science Society of Japan  2024.6 

    　More details

    Event date： 2024.6

    researchmap

  • Protein evolution by cell-free translation system in water-in-oil droplet

    Naohiro Terasaka

    The 24th Annual Meeting of the Protein Science Society of Japan  2024.6 

    　More details

    Event date： 2024.6

    researchmap

  • Fusion of U-body and a self-assembling protein produces a MET agonist

    Yuki Kashiwa, Katsuya Sakai, Naohiro Terasaka, Haruo Aikawa, Kunio Matsumoto, Hiroaki Suga

    The 3rd International Symposium on Biofunctional Chemistry  2024.4 

    　More details

    Event date： 2024.4

    researchmap

  • Directed evolution of virus-like ribonucleoprotein particles Invited

    Naohiro Terasaka

    The 3rd International Symposium on Biofunctional Chemistry  2024.4 

    　More details

    Event date： 2024.4

    researchmap

  • Development of nonviral nucleocapsid by laboratory evolution and its biotechnological application Invited

    Naohiro Terasaka

    The 144th Annual Meeting of the Pharmaceutical Society of Japan  2024.3 

    　More details

    Event date： 2024.3

    researchmap

  • Directed evolution of nonviral ribonucleoproteins to virus-like particles

    Naohiro Terasaka

    Joint CO world & 12th ELSI Symposium  2024.1 

    　More details

    Event date： 2024.1

    researchmap

  • In vitro assembly of protein capsule and cargo molecules into a virus-like particle

    2024.1 

    　More details

    Event date： 2024.1

    researchmap

  • Assembly of virus-like architectures directed by cargo molecules

    Kenya Tajima, Naohiro Terasaka

    The 61st Annual Meeting of the Biophysical Society of Japan  2023.11 

    　More details

    Event date： 2023.11

    researchmap

  • SURFACE FUNCTIONALIZATION OF A VIRUS-LIKE ARCHITECTURE WITH A PEPTIDE TAG

    Daiki Kanayama, Naohiro Terasaka, Hiroaki Suga

    The 60th Japanese Peptide Symposium  2023.11 

    　More details

    Event date： 2023.11

    researchmap

  • CHEMICAL MODIFICATION AT THE N-TERMINUS OF AMINOACYL-tRNA AND ITS APPLICATION TO IN VITRO TRANSLATION SYSTEM

    Hiroki Murakami, Naohiro Terasaka, Haruo Aikawa, Hiroaki Suga

    The 60th Japanese Peptide Symposium  2023.11 

    　More details

    Event date： 2023.11

    researchmap

  • Discovery of de novo macrocyclic peptide specifically binding to Siglec- 10 among Siglec family proteins

    Wei-En Huang, Naohiro Terasaka, Yuma Matsuzaki, Haruo Aikawa, Hiroaki Suga

    The 60th Japanese Peptide Symposium  2023.11 

    　More details

    Event date： 2023.11

    researchmap

  • Development of Bifunctional antibody with immune checkpoint inhibitory effect using Lasso-grafting

    Jinxuan ZHAO, Naohiro Terasaka, Yoji Murata, Hiroaki Suga

    The 60th Japanese Peptide Symposium  2023.11 

    　More details

    Event date： 2023.11

    researchmap

  • T-boxリボスイッチを基盤としたアミノアシル化リボザイムの構造と機能

    香川尊, 安部紘平, 石田啓, Wei Lu, 菅裕明, 寺坂尚紘, Adrian Ferré-D'Amar, 沼田倫征

    日本結晶学会年会  2023.10 

    　More details

    Event date： 2023.10

    researchmap

  • Development of bifunctional antibody with immune checkpoint inhibitory effect using Lasso-grafting

    Jinxuan ZHAO, Naohiro Terasaka, Yoji Murata, Hiroaki Suga

    13th International Peptide Symposium  2023.10 

    　More details

    Event date： 2023.10

    researchmap

  • Nucleic acids templated assembly of artificial virus-like particles

    Kenya Tajima, Yusuke Sakai, ○Naohiro Terasaka

    Japanese Society For Cell Synthesis Research annual meeting 16.0  2023.9 

    　More details

    Event date： 2023.9

    researchmap

  • Engineering and evolution of self-assembling proteins mimicking virus particles

    Naohiro Terasaka

    RIKEN Seminar  2023.7 

    　More details

    Event date： 2023.7

    researchmap

  • Development of aminoacylation ribozymes capable of peptide elongation in one-pot in vitro translation

    Wei Lu, Naohiro Terasaka, Hiroaki Suga

    IUBMB Focused Meeting on Aminoacyl-tRNA Synthetases 2023  2023.6 

    　More details

    Event date： 2023.6

    researchmap

  • 自己集合タンパク質に立脚した受容体チロシンキナーゼ 人工活性化剤の開発

    柏勇希, 酒井克也, 寺坂尚紘, 相川春夫, 松本邦夫, 菅裕明

    日本ケミカルバイオロジー学会第17回年会  2023.5 

    　More details

    Event date： 2023.5

    researchmap

  • RNAとタンパク質の協奏的進化による機能性複合体の創成 Invited

    寺坂尚紘

    自然科学研究機構 生命創成探究センター (ExCELLS)、分子科学研究所 (IMS)研究会「生命の分子システムの理解に向けて何を創れば良いか？」  2023.5 

    　More details

    Event date： 2023.5

    researchmap

  • Laboratory evolution of virus-like protein capsules

    Naohiro Terasaka, Kenya Tajima

    The 47th Annual Meeting of The Society for the Study of the Origin and Evolution of Life Japan  2023.3 

    　More details

    Event date： 2023.3

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • 環状γ-アミノ酸含有ペプチドを用いたSARS-CoV-2メインプロテアーゼ阻害剤のスクリーニング

    三浦 敬, Malla Tika, Owen David, Tumber Anthony, Brewitz Lennar, McDonough Michael, Salah Eidarus, 寺坂 尚紘, 加藤 敬行, Lukacik Petra, Strain-Damerell Claire, Mikolajek Halina, Walsh Martin, Kawamura Akane, Schofield Christopher, 菅 裕明

    日本薬学会第143年会  2023.3 

    　More details

    Event date： 2023.3

    researchmap

  • 環状βアミノ酸を含む新規ヘリカルペプチド薬剤候補探索

    川合 茉利奈, 加藤 敬行, Malla Tika, Tumber Anthony, Brewitz Lennar, McDonough Michael, Salah Eidraus, 寺坂 尚紘, Kawamura Akane, Schofield Christopher, 菅 裕明

    日本薬学会第143年会  2023.3 

    　More details

    Event date： 2023.3

    researchmap

  • アミノアシルtRNAの化学的N-末端修飾とin vitro翻訳系への応用

    The 103rd CSJ Annual Meeting  2023.3 

    　More details

    Event date： 2023.3

    researchmap

  • RaPIDセレクションを用いた免疫チェックポイントを阻害するペプチドの開発

    The 103rd CSJ Annual Meeting  2023.3 

    　More details

    Event date： 2023.3

    researchmap

  • 分子進化によるタンパク質・RNA 高次複合体の創成 Invited

    寺坂 尚紘

    第１回 発動分子科学サロン 「発動分子と生体材料」  2022.12 

    　More details

    Event date： 2022.12

    Language：Japanese  

    researchmap

  • In vitro assembly of protein capsules packaging RNA

    Naohiro Terasaka

    The 45th Annual Meeting of the Molecular Biology Society of Japan  2022.12 

    　More details

    Event date： 2022.11 - 2022.12

    researchmap

  • Aminoacylation ribozymes evolved from a natural riboswitch Invited

    Naohiro Terasaka

    Symposium on Evolution and Emergence of Living Systems: from Geochemistry to Synthetic Biology  2022.10 

    　More details

    Event date： 2022.10

    researchmap

  • In vitro reconstitution of artificial virus-like particles

    Naohiro Terasaka

    Japanese Society For Cell Synthesis Research annual meeting 15.0  2022.10 

    　More details

    Event date： 2022.10

    researchmap

  • Directed evolution of protein assembly Invited

    Naohiro Terasaka

    The 60th Annual Meeting of the Biophysical Society of Japan  2022.9 

    　More details

    Event date： 2022.9

    researchmap

  • 原始ウイルスの進化を実験室内で再現する Invited

    寺坂尚紘

    日本進化学会年大会 第24回沼津大会  2022.8 

    　More details

    Event date： 2022.8

    researchmap

  • Development of aminoacylation ribozyme by tRNA 3’ sensing in vitro selection strategy

    Wei Lu, Naohiro Terasaka, Hiroaki Suga

    23th RNA society Japan annual meeting  2022.7 

    　More details

    Event date： 2022.7

    researchmap

  • Construction of a peptide library containing cyclic γ-amino acids for discovery of SARS-CoV-2 main protease inhibitors

    Takashi Miura, Malla Tika, Owen David, Tumber Anthony, Brewitz Lennart, McDonough Michael, Salah Eidarus, Naohiro Terasaka, Takayuki Katoh, Lukacik Petra, Strain-Damerell Claire, Mikolajek Halina, Walsh Martin, Kawamura Akane, Schofield Christopher, Hiroaki Suga

    BIO UT 2022  2022.6 

    　More details

    Event date： 2022.6

    researchmap

  • Constructing a ribosome library with random mutations in peptidyl transferase center and screening ribosome sequences to incorporate noncanonical amino acids

    Daiki Kanayama, Takayuki Katoh, Naohiro Terasaka, Hiroaki Suga

    The 102nd CSJ Annual Meeting  2022.3 

    　More details

    Event date： 2022.3

    Presentation type：Poster presentation  

    researchmap

  • Development of Macrocyclic Peptide Heterodimer as PPI Inhibitor against Immune Checkpoint CD47- SIRPα

    Jinxuan Zhao, Yoji Murata, Naohiro Terasaka, Takashi Matozaki, Hiroaki Suga

    The 102nd CSJ Annual Meeting  2022.3 

    　More details

    Event date： 2022.3

    Presentation type：Oral presentation (general)  

    researchmap

  • Ribosomal synthesis of N-myristoylated peptide

    Hiroki Murakami, Naohiro Terasaka, Hiroaki Suga

    The 102nd CSJ Annual Meeting  2022.3 

    　More details

    Event date： 2022.3

    Presentation type：Oral presentation (general)  

    researchmap

  • Discovery of a cis-acting ribozyme conducting new type of reaction by in vitro selection

    Wei Lu, Naohiro Terasaka, Hiroaki Suga

    The 102nd CSJ Annual Meeting  2022.3 

    　More details

    Event date： 2022.3

    Presentation type：Poster presentation  

    researchmap

  • Laboratory evolution of a virus-like nucleocapsid from a bacterial enzyme

    Naohiro Terasaka, Hiroaki Suga, Donald Hilvert

    RIKEN BDR Symposium 2022  2022.3 

    　More details

    Event date： 2022.3

    Presentation type：Oral presentation (general)  

    researchmap

  • タンパク質カプセルへの環状ペプチド移植による肝細胞増殖因子受容体アゴニストの創成

    寺坂尚紘, 小松大和, 酒井克也, 三原恵美子, 若林里咲, 松本邦夫, Hilvert Donald, 高木淳一, 菅裕明

    第15回バイオ関連化学シンポジウム  2021.9 

    　More details

    Event date： 2021.9

    researchmap

  • Mutant ribosome?tRNA pair towards orthogonal genetic code International conference

    Terasaka N, Hayashi G, Katoh H, Suga H

    9th International Symposium on Aminoacyl-tRNA Synthetases  2013.10 

    　More details

    Event date： 2013.10

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Discovery of human small non-coding RNAs binding to small molecules by SELEX-NT International conference

    Terasaka N, Futai K, Katoh T, Suga H

    RiboClub Annual Meeting 2013  2013.9 

    　More details

    Event date： 2013.9

    Language：English   Presentation type：Poster presentation  

    researchmap

  • 変異リボソーム・tRNAペアを用いた直交性遺伝暗号の創成

    寺坂尚紘, 林剛介, 加藤敬行, 菅裕明

    第15回日本RNA学会年会  2013.7 

    　More details

    Event date： 2013.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    researchmap

  • Ribozymes that catalyze tRNA aminoacylation Invited International conference

    Terasaka N, Ishida S, Katoh T, Suga H

    Pacifichem 2015  2015.12 

    　More details

    Language：English   Presentation type：Oral presentation (invited, special)  

    researchmap

  • A T-box ribozyme with tRNA amioacylation activity International conference

    Ishida S, Terasaka N, Katoh T, Suga H

    10th International Symposium on Aminoacyl-tRNA Synthetase (aaRS2015)  2015.10 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center International conference

    Terasaka N, Hayashi G, Katoh H, Suga H

    The 3rd Japan-Swiss Chemical Biology Symposium  2014.10 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • An orthogonal ribosome-tRNAs pair by the engineering of peptidyl transferase center International conference

    Terasaka N, Hayashi G, Katoh H, Suga H

    25th tRNA conference  2014.9 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • 天然リボソーム・tRNAペアと直交性を持つ変異リボソーム・tRNAペアの構築

    寺坂尚紘, 林剛介, 加藤敬行, 菅裕明

    日本ケミカルバイオロジー学会第9回年会  2014.6 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

    researchmap

  • In vitroセレクションを用いて小分子結合性small non-coding RNAを探索する

    寺坂尚紘, 二井一樹, 加藤敬行, 菅裕明

    第16回生命化学研究会  2014.1 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • The translational activity of a CCA-mutated tRNA-ribosome mutant pair International conference

    Terasaka N, Suga H

    Annual Symposium on Academic English for Chemistry  2013.2 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Translational activity of CCA-mutated tRNA-ribosome mutant pair. International conference

    Terasaka N, Hayashi G, Katoh H, Suga H

    XXIV tRNA conference  2012.12 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • 変異リボソーム・tRNAペアによる直交性遺伝暗号の創成

    寺坂尚紘, 林剛介, 加藤敬行, 菅裕明

    RNAフロンティアミーティング2012  2012.9 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

    researchmap

  • In vitroセレクションによる生体内小分子に結合するヒトsmall non-coding RNAの探索

    寺坂尚紘, 二井一樹, 加藤敬行, 後藤佑樹, 菅裕明

    第24回高遠シンポジウム  2012.8 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • In vitroセレクションを用いた生体内小分子に結合するヒトsmall non-coding RNAの探索

    寺坂尚紘, 二井一樹, 加藤敬行, 後藤佑樹, 菅裕明

    第14回日本RNA学会年会  2012.7 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • 生体内小分子に結合するヒトsmall non-coding RNAの探索

    寺坂尚紘, 二井一樹, 加藤敬行, 菅裕明

    新規素材探索第11回セミナー  2012.6 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • in vitro セレクションによる小分子結合性small non-coding RNAの探索

    寺坂尚紘, 加藤敬行, 菅裕明

    第34回日本分子生物学会年会  2011.12 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • RaPID システムを利用して環状N-メチルペプチド阻害剤を探索する

    山岸祐介, 寺坂尚紘, 菅裕明

    第13回生命化学研究会シンポジウム（冬季  2011.1 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • アーキアtRNAIleに見出された新規RNA修飾塩基アグマチジンの機能と生合成機構の解明

    池内与志穂, 木村 聡, 寺坂尚紘, 沼田倫征, 大澤拓生, 中村大吾, 横川隆志, 緒方俊彦, 和田 猛, 鈴木健夫, 鈴木 勉

    第12回日本RNA学会年会  2010.7 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • RNA・タンパク質カプシド複合体の実験室内進化

    寺坂尚紘, 東佑翼, Tetter Stephan, 菅 裕明, Hilvert Donald

    第41回日本分子生物学会年会  2018.11 

    　More details

    Language：Japanese   Presentation type：Oral presentation (general)  

    researchmap

  • ウイルスを模倣した人工ヌクレオカプシドの実験室内進化

    寺坂尚紘, 東佑翼, Tetter Stephan, Hilvert Donald

    「細胞を創る」研究会11.0  2018.10 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • Engineering and directed evolution of virus-like nucleocapsids International conference

    Terasaka N, Azuma Y, Hilvert D

    2nd ETH Zurich The University of Tokyo Strategic Partnership Symposium on Science, Design, Manufacturing and Innovation,  2018.1 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • T-boxリボスイッチに基づいたtRNA認識アミノアシル化リボザイムの開発

    Ishida S, Terasaka N, Katoh T, Suga H

    第5回バイオ関連化学シンポジウム若手フォーラム  2017.9 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • In vitro selection法によるtRNA認識とアミノアシル活性を併せ持ったリボザイムの開発

    Ishida S, Terasaka N, Katoh T, Suga H

    第11回バイオ関連化学シンポジウム  2017.9 

    　More details

    Language：Japanese   Presentation type：Poster presentation  

    researchmap

  • In vivo packaging of small RNAs into engineered nonviral protein capsids International conference

    Terasaka N, Edwardson T.G.W, Azuma Y, Hilvert D

    Gordon Research Conference on Physical Virology  2017.1 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • Ribozymes with tRNA recognition and aminoacylation properties” 26th tRNA conference International conference

    Ishida S, Terasaka N, Katoh T, Suga H

    26th tRNA conference  2016.9 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • Microfluidic cell sorter-aided directed evolution of box C/D snoRNP to site-specifically introduce N6-methyladenosine International conference

    Terasaka N, Hilvert D

    16th HFSP meeting  2016.7 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • Microfluidic compartmentalized directed evolution of box C/D snoRNP to methylate RNA bases International conference

    Terasaka N, Hilvert D

    RNA 2016  2016.6 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • In vitro selection of tRNA-recognizing aminoacylation ribozyme derived from a T-box motif International conference

    Ishida S, Terasaka N, Katoh T, Suga H

    RNA 2016  2016.6 

    　More details

    Language：English   Presentation type：Oral presentation (general)  

    researchmap

  • An aminoacylation ribozyme evolved from a natural tRNA sensing riboswitch Invited

    2020.12 

    　More details

  • RANKL-RANK-OPGシグナル研究の最前線 膜型RANKLを標的にした骨形成促進薬の開発

    青木 和広, 清水 優里, Lu Wei, 廣橋 優奈, 曽根 絵梨, 池淵 祐樹, Masud Khan, Fatma Rashed, 田村 幸彦, 菅森 泰隆, 寺坂 尚紘, 宇田川 信之, 依田 哲也, 本間 雅, 菅 裕明

    第62回歯科基礎医学会学術大会 アップデートシンポジウム  2020.10 

    　More details

  • 天然リボスイッチ配列から進化させ、翻訳系中で働くアミノア シル tRNA合成リボザイム

    寺坂 尚紘, 石田啓, 加藤敬行, 菅裕明

    第42回日本分子生物学会年会  2019.12 

    　More details

  • T-box リボスイッチをベースにした tRNA 認識アミノアシル化リボザイムの創出

    石田啓, 寺坂 尚紘, 加藤敬行, 菅裕明

    第21回日本RNA学会年会  2019.7 

    　More details

  • Directed evolution of non-viral protein cages to package their own mRNA

    Terasaka Naohiro, Azuma Yusuke, Tetter Stephan, Hiroaki Suga, Hilvert Donald

    JSCB-PSSJ2019  2019.6 

    　More details

    Presentation type：Oral presentation (invited, special)  

    researchmap

  • An aminoacylation ribozyme evolved from a natural tRNA sensing riboswitch International conference

    Terasaka Naohiro, Ishida Satoshi, Katoh Takayuki, Suga Hiroaki

    The 24th Annual Meeting of the RNA Society  2019.6 

    　More details

    Language：English   Presentation type：Poster presentation  

    researchmap

  • Application of BLI for discovery of natural RNA aptamers

    Terasaka Naohiro

    2019.5 

    　More details

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    researchmap

  • Reconstruction of virus origin in a laboratory Invited

    Terasaka Naohiro

    Keio Astrobiology Camp 2019  2019.3 

    　More details

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    researchmap

  • Directed evolution of bacterial protein cages to package their own mRNA Invited

    Terasaka Naohiro

    New Frontier in Protein Design & Engineering  2019.3 

    　More details

    Language：English   Presentation type：Oral presentation (invited, special)  

    researchmap

  • De novo macrocyclic peptide inhibitors containing cyclic γ-amino acids targeting the SARS-CoV-2 main protease

    Takashi Miura, Malla Tika, Owen Davi, Tumber Anthony, Brewitz Lennar, McDonough Michael, Salah Eidarus, Naohiro Terasaka, Takayuki Katoh, Lukacik Petr, Strain-Damerell Claire, Mikolajek Halin, Walsh Marti, Kawamura Akane, Schofield Christopher, Hiroaki Suga

    Boulder Peptide Symposium 2022  2022.11 

    　More details

  • Ribosomal synthesis of a peptide library containing cyclic γ-amino acids for discovery of SARS-CoV-2 main protease inhibitors

    Takashi Miura, Malla Tika, Owen Davi, Tumber Anthony, Brewitz Lennar, McDonough Michael, Salah Eidarus, Naohiro Terasaka, Takayuki Katoh, Lukacik Petr, Strain-Damerell Claire, Mikolajek Halin, Walsh Marti, Kawamura Akane, Schofield Christopher, Hiroaki Suga

    The 59th Japanese Peptide Symposium  2022.10 

    　More details

  • Screening de novo RNA-binding peptides using codon-depleted mRNA display

    Kosuke Fujishima, Shota Nishikawa, Hidenori Watanabe, Naohiro Terasaka, Takayuki Katoh

    2022 Astrobiology Science Conference  2022.5 

    　More details

  • In vitro evolution of aminoacylation ribozymes and their applications

    Naohiro Terasaka, Satoshi Ishida, Takayuki Katoh, Kazuki Futai, Wei Lu, Hiroaki Suga

    2021.12 

    　More details

▼display all

Awards

  • Young Scientist Award

    2019.6   Protein Science Society Japan  

    Terasaka Naohiro

    　More details

  • 東京大学大学院理学系研究科研究奨励賞受賞

    2015.3  

    寺坂 尚紘

    　More details

  • 25th tRNA conference, Sidney Altman Endowment Travel Award 受賞

    2014.9  

    寺坂 尚紘

    　More details

  • 第15回日本RNA学会年会優秀賞受賞

    2013.7  

    寺坂 尚紘

    　More details

  • 第63回リンダウ・ノーベル賞受賞者会議に日本学術振興会より派遣

    2013.6  

    寺坂 尚紘

    　More details

Research Projects

  • ボトムアップ合成ウイルス学

    2025.4 - 2028.3

    科学技術振興機構  創発的研究支援事業 

    　 More details

  • 天然を超えた効率的物質生産を可能にする人工特化メタボロンの創成

    Grant number：25H01420  2025.4 - 2028.3

    日本学術振興会  科学研究費助成事業  学術変革領域研究(B)

    寺坂 尚紘

    　 More details

    Grant amount：\35750000 （ Direct Cost: \27500000 、 Indirect Cost：\8250000 ）

    researchmap

  • 多様なモダリティの薬剤分子を迅速創出する無細胞プラットフォームの構築

    Grant number：25K01913  2025.4 - 2028.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    寺坂 尚紘

    　 More details

    Grant amount：\18720000 （ Direct Cost: \14400000 、 Indirect Cost：\4320000 ）

    researchmap

  • マイクロ流路デバイスを用いたセルフリータンパク質進化

    2025.4 - 2026.3

    公益財団法人豊田理化学研究所  豊田理研スカラー共同研究 (Phase1) 

    　 More details

  • 機能性核酸ペプチド複合体進化システムの構築と原始翻訳系の解明

    2024.7 - 2029.3

    武田科学振興財団  生命科学研究助成 

    　 More details

  • 多様な薬剤の迅速探索を可能にする無細胞連続進化システムの構築

    2024.4 - 2027.3

    公益財団法人内藤記念科学振興財団  2023年度内藤記念次世代育成支援研究助成金 

    　 More details

  • 核酸タンパク質複合体を用いた細胞内ナノ構造体構築

    2024.4 - 2027.3

    独立行政法人日本学術振興会  挑戦的研究（萌芽） 

    　 More details

    Authorship：Principal investigator 

    researchmap

  • 高純度mRNA薬剤の合成を可能にするRNAポリメラーゼの開発

    2024.4 - 2025.3

    中外創薬科学財団  特別研究助成金 SRG2022 

    　 More details

  • 受容体クラスター化アゴニストの指向性進化

    2024.1 - 2025.12

    公益財団法人アステラス病態代謝研究会 研究助成 

    　 More details

  • 抗菌ペプチドの微量迅速探索法の開発

    2024.1 - 2024.12

    公益財団法人 持田記念医学薬学振興財団  2023年度研究助成 

    　 More details

  • 抗菌ペプチド無細胞スクリーニング系の開発

    2024.1 - 2024.12

    シオノギ感染症研究振興財団  次世代育成支援研究助成金 

    　 More details

  • Development of RNA-guided programmed cell death technology using CRISPR-Cas7-11

    2023.7 - 2024.3

    Kazuki Kato, Naohiro Terasaka

    　 More details

    Authorship：Coinvestigator(s) 

    researchmap

  • 多量体化人工抗体によるMETを標的とした人工成長因子の創成

    2022.4 - 2023.3

    金沢大学がん進展制御研究所 共同研究 

    　 More details

  • 相分離進化工学による人工オルガネラの創成

    2021.10 - 2025.3

    科学技術振興機構  戦略的創造研究推進事業（さきがけ） 

    　 More details

  • 非天然アミノ酸を含む高次機能性タンパク質集合体の極微量分子進化

    2021.10 - 2024.3

    独立行政法人日本学術振興会  学術変革領域研究B 

    　 More details

  • METをターゲットとする小分子薬剤・タンパク質複合体の構築

    2020.4 - 2021.3

    金沢大学がん進展制御研究所  共同研究 

    寺坂尚紘, 松本邦夫

    　 More details

    Authorship：Principal investigator 

    researchmap

  • 標的特異的ドラッグデリバリーを可能にするタンパク質カプセルの分子進化

    2020.4 - 2021.3

    公益財団法人 野口研究所  野口遵研究助成金 

    寺坂尚紘

    　 More details

    Authorship：Principal investigator 

    researchmap

  • 分子進化によるウイルス細胞脱出起源仮説の再現

    2020.4 - 2021.3

    自然科学研究機構アストロバイオロジーセンター  プロジェクト研究 

    寺坂尚紘

    　 More details

    Authorship：Principal investigator 

    researchmap

  • tRNAのアミノアシル化を触媒するペプチド・リボザイム複合体の分子進化

    2019.4 - 2022.3

    独立行政法人日本学術振興会  若手研究 

    寺坂 尚紘

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    researchmap

  • 分子進化工学によるウイルス出芽機構の解明

    2019.4 - 2020.3

    公益財団法人豊田理化学研究所  豊田理研スカラー 

    寺坂 尚紘

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    researchmap

  • HGF-MET系をターゲットとしたドラッグデリバリーシステムの開発

    2019.4 - 2020.3

    金沢大学がん進展制御研究所  金沢大学がん進展制御研究所共同研究 

    寺坂 尚紘

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    researchmap

  • 抗HGF特殊環状ペプチドのイメージング活用創薬

    2016.4 - 2021.3

    国立研究開発法人 日本医療研究開発機構  次世代がん医療創生研究事業（P-CREATE） 

    寺坂尚紘

    　 More details

    Authorship：Coinvestigator(s) 

    researchmap

  • Site-specific introduction of N6-methyladenosine using engineered snoRNP by directed evolution

    2015.4 - 2018.3

    ヒューマン・フロンティア・サイエンス・プログラム（HFSP): 長期フェロー 

    寺坂 尚紘

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    researchmap

  • 生体内小分子によって成熟が制御されるヒトmiRNA前駆体の探索及び機能解析

    2012.4 - 2014.3

    独立行政法人日本学術振興会: 特別研究員（DC1） 

    寺坂 尚紘

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    researchmap

▼display all

Other

  • 日本学術振興会海外特別研究員 採用内定（辞退）

    2015.4

    　More details

  • 日本学生支援機構大学院第一種奨学生 特に優れた業績による返還免除

    2012.3

    　More details

Teaching Experience

  • Synnthetic Biology

    2023.6 Institution：Tokyo Institute of Technology

    　More details

  • Earth-Life Science A

    2023.4 Institution：Tokyo Institute of Technology

    　More details

  • 有機化学実験

    2019.10 Institution：東京大学理学部化学科

    　More details

  • Biological Chemistry I: Nucleic Acids & Carbohydrates

    　More details

  • 有機化学演習

    　More details

Social Activities

  • 横浜サイエンスフロンティア高校生への講演

    Role(s)： Lecturer

    2025.9

    　More details

    Type：Lecture

    researchmap

  • 都立墨田川高校 大学模擬授業

    Role(s)： Lecturer

    2024.10

    　More details

    Type：Visiting lecture

    researchmap

  • 東京工業大学 地球生命研究所 一般講演会 2024

    Role(s)： Presenter

    2024.2

    　More details

  • 都立竹早高校生への講演

    Role(s)： Lecturer

    2023.7

    　More details

Academic Activities

  • 機能性生体分子の合理デザイン（第98回日本生化学会大会）

    Role(s)： Panel moderator, session chair, etc.

    2025.11

    　More details

  • 異分野融合が切り拓くタンパク質工学フロンティア（第24回日本蛋白質科学会年会ワークショップ）

    Role(s)： Panel moderator, session chair, etc.

    2024.6

    　More details

  • 日本生物物理学会 分野別専門委員

    2024.1

    　More details

  • ELSI seminar

    Role(s)： Planning, management, etc.,　Panel moderator, session chair, etc.

    Naohiro Terasaka  2023.7

    　More details

    Type：Academic society, research group, etc. 

    researchmap

  • ACCELERATING PROTEIN DESIGN AND ENGINEERING (Symposium in the 23rd Annual Meeting of the Protein Science Society of Japan)

    Role(s)： Planning, management, etc.,　Panel moderator, session chair, etc.

    Yuta Suzuki, Naohiro Terasaka  2023.7

    　More details

    Type：Competition, symposium, etc. 

    researchmap

  • SPEED×Bottom-up×ELSI joint workshop

    Role(s)： Planning, management, etc.,　Panel moderator, session chair, etc.

    Naohiro Terasaka  2023.7

    　More details

    Type：Academic society, research group, etc. 

    researchmap

  • NISTEP分野別専門委員

    2020.4

    　More details

▼display all