Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

Abstract

Abiotic and biotic stresses pose serious threats to plant productivity. Elucidating the gene regulatory networks involved in plant stress responses is essential for developing future breeding programs and innovative agricultural products. Here, we introduce the AtSRGA (Arabidopsis thaliana  Stress Responsive Gene Atlas), a user-friendly application facilitating the retrieval of stress-responsive genes in Arabidopsis (Arabidopsis thaliana). The application was developed using 1,131 microarray and 1,050 RNA sequencing datasets obtained from public databases. These datasets correspond to 11 stress-related conditions, namely abscisic acid, cold, drought, heat, high light, hypoxia, osmotic stress, oxidative stress, salt, wounding, and Pseudomonas syringae pv. tomato DC3000. Using a modified meta-analysis technique known as the vote-counting method, we computed integrated scores to evaluate stress responsiveness for each condition across multiple studies. AtSRGA visualizes gene behavior under 11 stress conditions and offers an interactive, user-friendly interface accessible to all researchers. It presents a comprehensive heatmap of stress-responsive genes, facilitating the comparative analysis of individual stress responses and groups of genes responding to multiple stresses. We validated the expression patterns of several high-scoring genes of unknown function under cold and heat stress using RT-qPCR, thus demonstrating that our application helps select targets to understand stress-responsive gene networks in Arabidopsis. AtSRGA will improve the screening of stress-responsive genes in Arabidopsis, thereby supporting the advancement of plant science toward a sustainable society.

DOI： 10.1093/plphys/kiaf105

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Abstract

DNA double‐strand breaks (DSBs) are the most severe DNA lesions and need to be removed immediately to prevent loss of genomic information. Recently, it has been revealed that DSBs induce novel transcription from the cleavage sites in various species, resulting in RNAs being referred to as damage‐induced RNAs (diRNAs). While diRNA synthesis is an early event in the DNA damage response and plays an essential role in DSB repair activation, the location where diRNAs are newly generated in plants remains unclear, as does their transcriptional mechanism. Here, we performed the sequencing of polyadenylated (polyA) diRNAs that emerged around all DSB loci in Arabidopsis thaliana under the expression of the exogenous restriction enzyme Sbf I and observed 88 diRNAs transcribed via RNA polymerase II in 360 DSB loci. Most of the detected diRNAs originated within active genes and were transcribed from DSBs in a bidirectional manner. Furthermore, we found that diRNA elongation tends to terminate at the boundary of an endogenous gene located near DSB loci. Our results provide reliable evidence for understanding the importance of new transcription at DSBs and show that diRNA is a crucial factor for successful DSB repair.

DOI： 10.1111/gtc.13133

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DNA double-strand breaks (DSBs) are among the most serious types of DNA damage, causing mutations and chromosomal rearrangements. In eukaryotes, DSBs are immediately repaired in coordination with chromatin remodeling for the deposition of DSB-related histone modifications and variants. To elucidate the details of DSB-dependent chromatin remodeling throughout the genome, artificial DSBs need to be reproducibly induced at various genomic loci. Recently, a comprehensive method for elucidating chromatin remodeling at multiple DSB loci via chemically induced expression of a restriction enzyme was developed in mammals. However, this DSB induction system is unsuitable for investigating chromatin remodeling during and after DSB repair, and such an approach has not been performed in plants. Here, we established a transgenic Arabidopsis plant harboring a restriction enzyme gene Sbf I driven by a heat-inducible promoter. Using this transgenic line, we performed chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) of histones H4K16ac and H2A.Z and investigated the dynamics of these histone marks around the endogenous 623 Sbf I recognition sites. We also precisely quantified DSB efficiency at all cleavage sites using the DNA resequencing data obtained by the ChIP-seq procedure. From the results, Sbf I-induced DSBs were detected at 360 loci, which induced the transient deposition of H4K16ac and H2A.Z around these regions. Interestingly, we also observed the co-localization of H4K16ac and H2A.Z at some DSB loci. Overall, DSB-dependent chromatin remodeling was found to be highly conserved between plants and animals. These findings provide new insights into chromatin remodeling that occurs in response to DSBs in Arabidopsis.

DOI： 10.1093/pcp/pcad133

PubMed

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Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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Event date： 2025.9

Language：English   Presentation type：Poster presentation  

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Event date： 2025.6

Language：English   Presentation type：Poster presentation  

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Event date： 2025.4

Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Event date： 2025.3

Language：English   Presentation type：Oral presentation (general)  

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Event date： 2024.11

Language：English   Presentation type：Poster presentation  

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