Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.devcel.2023.12.014

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acschembio.3c00478

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Publisher：Cold Spring Harbor Laboratory  

Cyclin-dependent kinase (CDK) plays an essential role in determining the temporal ordering of the cell cycle phases. However, despite significant progress in studying regulators of CDK, it remains elusive how they coordinately affect CDK activity at the single-cell level and how CDK controls the temporal order of cell cycle events. This could be due to the lack of tools to monitor CDK activity in living cells. Here, we elucidate the dynamics of CDK activity in fission yeast and mammalian cells by using a newly developed CDK activity biosensor, Eevee-spCDK, based on Forster Resonance Energy Transfer (FRET). Taking advantage of this system, we unravel the profile of CDK activity in vegetatively growing S. pombe cells. Thus, we detect a transient increase in S phase followed by a gradual increment during G2 phase. CDK activity then reaches its maximum in early M phase and rapidly decreases at mitotic exit. During G2 phase, CDK activity exhibits a biphasic pattern, i.e., an early slow increase and a late fast rise prior to the G2/M phase transition, as predicted from mathematical studies. Remarkably, although CDK activity does not necessarily correlate with cyclin levels, we find that it converges to the same level around mitotic onset in several mutant backgrounds, including pom1Δ cells and wee1 or cdc25 overexpressing cells. These data provide the first direct evidence that cells enter M phase when CDK activity reaches a high threshold, consistent with the quantitative model of cell cycle progression in fission yeast.

DOI： 10.1101/2023.03.26.534249

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society ({ACS})  

DOI： 10.1021/acs.langmuir.1c02103

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

DOI： 10.1016/j.cocis.2020.04.003

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Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsomega.0c01371

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s42004-020-0277-2

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.5360/membrane.44.63

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Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

Abstract

The self-reproduction of supramolecular assemblies based on the synthesis and self-assembly of building blocks is a critical step towards the construction of chemical systems with autonomous, adaptive, and propagation properties. In this report, we demonstrate that giant vesicles can grow and produce daughter vesicles by synthesizing and incorporating phospholipids in situ from ad-hoc precursors. Our model involves acyl chain elongation via copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition reaction and the ensuing production of synthetic phospholipids to induce budding and division. In addition, the growth and budding of giant vesicles were compatible with the encapsulation and transfer of macromolecules as large as lambda phage DNA to the buds. This chemical system provides a useful model towards the implementation of cell-like compartments capable of propagation and transport of biological materials.

DOI： 10.1038/s41598-018-36183-9

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Other Link： https://www.nature.com/articles/s41598-018-36183-9.pdf

Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Chemical and Biological Microsystems Society  

Scopus

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Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Experimental evolution in chemical models of cells could reveal the fundamental mechanisms of cells today. Various chemical cell models, water-in-oil emulsions, oil-on-water droplets, and vesicles have been constructed in order to conduct research on experimental evolution. In this review, firstly, recent studies with these candidate models are introduced and discussed with regards to the two hierarchical directions of experimental evolution (chemical evolution and evolution of a molecular self-assembly). Secondly, we suggest giant vesicles (GVs), which have diameters larger than 1 µm, as promising chemical cell models for studying experimental evolution. Thirdly, since technical difficulties still exist in conventional GV experiments, recent developments of microfluidic devices to deal with GVs are reviewed with regards to the realization of open-ended evolution in GVs. Finally, as a future perspective, we link the concept of messy chemistry to the promising, unexplored direction of experimental evolution in GVs.

DOI： 10.3390/life8040053

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Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)  

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Event date： 2023.8 - 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2023.7

Language：English   Presentation type：Oral presentation (general)  

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Event date： 2023.7

Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Event date： 2023.6

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2023.5 - 2023.6

Language：English   Presentation type：Oral presentation (general)  

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Event date： 2022.11 - 2022.12

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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Event date： 2022.9

Language：English   Presentation type：Oral presentation (general)  

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Event date： 2022.9

Language：English   Presentation type：Oral presentation (general)  

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Event date： 2022.6

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2021.12

Language：English   Presentation type：Oral presentation (invited, special)  

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Event date： 2021.11

Language：English   Presentation type：Oral presentation (invited, special)  

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Event date： 2021.10

Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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Event date： 2021.8 - 2021.9

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2020.11

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2020.10

Language：English   Presentation type：Symposium, workshop panel (nominated)  

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Event date： 2020.9

Language：English   Presentation type：Poster presentation  

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Event date： 2020.7

Language：English   Presentation type：Poster presentation  

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Event date： 2020.3

Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Event date： 2019.11

Language：English   Presentation type：Symposium, workshop panel (nominated)  

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Event date： 2019.10

Language：English   Presentation type：Poster presentation  

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Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2019.8

Language：English   Presentation type：Poster presentation  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Poster presentation  

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