Faculty Profiles - SATO SHUSUKE

写真a

SATO SHUSUKE

Organization

School of Science Assistant Professor

External link

Degree

博士（理学） ( 2021.3 東京工業大学理学院化学系化学コース )

Research Interests

Enzymology
Natural product
Biosynthesis
Radical SAM enzyme
hopanoid
fosfomycin
Apramycin
Herbicidins

Research Areas

Life Science / Bioorganic chemistry / Natural products
Life Science / Structural biochemistry / Enzymology

Education

Tokyo Institute of Technology School of Science Department of Chemistry

2018.4 - 2021.3

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Country： Japan

Notes： Doctor of Science

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Tokyo Institute of Technology School of Science Department of Chemistry

2016.4 - 2018.3

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Country： Japan

Notes： Master of Science

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Tokyo Institute of Technology School of Science Department of Chemistry

2011.4 - 2016.3

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Country： Japan

Notes： Bachelor of Science

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Research History

Institute of Science Tokyo Department of Chemistry Assistant Professor

2024.10

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Country：Japan

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The University of Texas at Austin Division of Chemical Biology and Medicinal Chemistry Postdoctoral fellow

2021.10 - 2024.9

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Country：United States

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Tokyo Institute of Technology Department of Chemistry Postdoc

2021.4 - 2021.9

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Country：Japan

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Tokyo Institute of Technology Department of Chemistry Research Fellowship for Young Scientist (DC2)

2019.4 - 2021.3

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Country：Japan

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Professional Memberships

日本農芸化学会

2019.3

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日本化学会

2016.12

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Papers

Complete In Vitro Reconstitution of the Apramycin Biosynthetic Pathway Demonstrates the Unusual Incorporation of a β-D-Sugar Nucleotide in the Final Glycosylation Step Reviewed

Shusuke Sato, Po-Hsun Fan, Yu-Cheng Yeh, Hung-wen Liu

Journal of the American Chemical Society 146 ( 14 ) 10103 - 10114 2024.4

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1021/jacs.4c01233

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Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis Invited

Shusuke Sato, Fumitaka Kudo, Tadashi Eguchi

Methods in Enzymology 669 45 - 70 2022.1

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Authorship：Lead author Language：English Publishing type：Part of collection (book) Publisher：Elsevier

DOI： 10.1016/bs.mie.2021.11.025

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Characterization of Radical SAM Adenosylhopane Synthase, HpnH, which Catalyzes the 5′‐Deoxyadenosyl Radical Addition to Diploptene in the Biosynthesis of C₃₅ Bacteriohopanepolyols Reviewed

Shusuke Sato, Fumitaka Kudo, Michel Rohmer, Tadashi Eguchi

Angewandte Chemie International Edition 59 ( 1 ) 237 - 241 2019.11

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Adenosylhopane is a crucial intermediate in the biosynthesis of bacteriohopanepolyols, which are widespread prokaryotic membrane lipids. Herein, it is demonstrated that reconstituted HpnH, a putative radical S‐adenosyl‐l‐methionine (SAM) enzyme, commonly encoded in the hopanoid biosynthetic gene cluster, converts diploptene into adenosylhopane in the presence of SAM, flavodoxin, flavodoxin reductase, and NADPH. NMR spectra of the enzymatic reaction product were identical to those of synthetic (22R)‐adenosylhopane, indicating that HpnH catalyzes stereoselective C−C formation between C29 of diploptene and C5′ of 5′‐deoxyadenosine. Further, the HpnH reaction in D₂O‐containing buffer revealed that a D atom was incorporated at the C22 position of adenosylhopane. Based on these results, we propose a radical addition reaction mechanism catalyzed by HpnH for the formation of the C₃₅ bacteriohopane skeleton.

DOI： 10.1002/anie.201911584

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.201911584
C-Methylation Catalyzed by Fom3, a Cobalamin-Dependent Radical S-adenosyl-L-methionine Enzyme in Fosfomycin Biosynthesis, Proceeds with Inversion of Configuration Reviewed

Shusuke Sato, Fumitaka Kudo, Tomohisa Kuzuyama, Friedrich Hammerschmidt, Tadashi Eguchi

Biochemistry 57 ( 33 ) 4963 - 4966 2018.7

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.biochem.8b00614

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Antifungal Cyclic Peptides Dinemasporins from the Culture of Dinemasporium parastrigosum KT4144 Reviewed

Kosei Kirisawa, Ryuhi Kanehara, Kazuaki Tanaka, Shusuke Sato, Hayato Maeda, Katsuhiro Konno, Atsushi Minami, Masaru Hashimoto

Journal of Natural Products 2026.3

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.jnatprod.6c00226

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Structure-guided switching of carbocation quenching in sesquiterpene synthases for accessing aromadendrane and guaiane frameworks Reviewed

Atsushi Suwa, Shusuke Sato, Atsushi Minami

Bulletin of the Chemical Society of Japan 99 ( 3 ) uoag032 2026.3

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1093/bulcsj/uoag032

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4″-Methyl-S-Adenosyl-L-Methionines Are Substrates for 4-Methylazetidinecarboxylic Acid Synthases Reviewed

Nayuta Nagano, Koichi Kiuchi, Atsushi Minato, Shusuke Sato, Yoshitaka Moriwaki, Rolf Müller, Tadashi Eguchi, Fumitaka Kudo

ACS Chemical Biology 21 ( 2 ) 224 - 229 2026.1

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acschembio.5c00642

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Isolation and Identification of Xanthone, Benzofuran, and Spirolactone Derivatives from Dinemasporium parastrigosum KT4144 and Their Plausible Biosynthetic Pathways Reviewed

Kosei Kirisawa, Shusuke Sato, Atsushi Minami, Hayato Maeda, Kazuaki Tanaka, Masaru Hashimoto

Journal of Natural Products 88 ( 6 ) 1453 - 1462 2025.5

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.jnatprod.5c00376

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Chain-length preference of trans-acting enoylreductases involved in the biosynthesis of fungal polyhydroxy polyketides Reviewed

Yuichiro Takekawa, Junya Takino, Shusuke Sato, Hideaki Oikawa, Toyoyuki Ose, Atsushi Minami

Biochemical and Biophysical Research Communications 761 151737 - 151737 2025.5

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Publishing type：Research paper (scientific journal) Publisher：Elsevier BV

DOI： 10.1016/j.bbrc.2025.151737

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Biosynthetic Origin of the Octose Core and Its Mechanism of Assembly during Apramycin Biosynthesis Reviewed

Po-Hsun Fan, Shusuke Sato, Yu-Cheng Yeh, Hung-wen Liu

Journal of the American Chemical Society 145 ( 39 ) 21361 - 21369 2023.9

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/jacs.3c06354

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Mechanism of S-Adenosyl-L-methionine C-Methylation by Cobalamin-dependent Radical S-Adenosyl-L-methionine Methylase in 1-Amino-2-methylcyclopropanecarboxylic Acid Biosynthesis Reviewed

Fumitaka Kudo, Atsushi Minato, Shusuke Sato, Nayuta Nagano, Chitose Maruyama, Yoshimitsu Hamano, Junko Hashimoto, Ikuko Kozone, Kazuo Shin-ya, Tadashi Eguchi

Organic Letters 24 ( 49 ) 8975 - 8979 2022.12

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.orglett.2c03555

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Identification of the Early Steps in Herbicidin Biosynthesis Reveals an Atypical Mechanism of C-Glycosylation Reviewed

Zhang Chen, Shusuke Sato, Yujie Geng, Jiawei Zhang, Hung-wen Liu

Journal of the American Chemical Society 144 ( 34 ) 15653 - 15661 2022.8

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/jacs.2c05728

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Biochemical and Mutational Analysis of Radical S-Adenosyl-L-Methionine Adenosylhopane Synthase HpnH from Zymomonas mobilis Reveals that the Conserved Residue Cysteine-106 Reduces a Radical Intermediate and Determines the Stereochemistry Reviewed

Shusuke Sato, Fumitaka Kudo, Michel Rohmer, Tadashi Eguchi

Biochemistry 60 ( 38 ) 2865 - 2874 2021.9

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.biochem.1c00536

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C-Methylation of S-adenosyl-L-Methionine Occurs Prior to Cyclopropanation in the Biosynthesis of 1-Amino-2-Methylcyclopropanecarboxylic Acid (Norcoronamic Acid) in a Bacterium Reviewed

Chitose Maruyama, Yukiko Chinone, Shusuke Sato, Fumitaka Kudo, Kosuke Ohsawa, Junya Kubota, Junko Hashimoto, Ikuko Kozone, Takayuki Doi, Kazuo Shin-ya, Tadashi Eguchi, Yoshimitsu Hamano

Biomolecules 10 ( 5 ) 775 - 775 2020.5

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Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Many pharmacologically important peptides are bacterial or fungal in origin and contain nonproteinogenic amino acid (NPA) building blocks. Recently, it was reported that, in bacteria, a cyclopropane-containing NPA 1-aminocyclopropanecarboxylic acid (ACC) is produced from the L-methionine moiety of S-adenosyl-L-methionine (SAM) by non-canonical ACC-forming enzymes. On the other hand, it has been suggested that a monomethylated ACC analogue, 2-methyl-ACC (MeACC), is derived from L-valine. Therefore, we have investigated the MeACC biosynthesis by identifying a gene cluster containing bacterial MeACC synthase genes. In this gene cluster, we identified two genes, orf29 and orf30, which encode a cobalamin (B12)-dependent radical SAM methyltransferase and a bacterial ACC synthase, respectively, and were found to be involved in the MeACC biosynthesis. In vitro analysis using their recombinant enzymes (rOrf29 and rOrf30) further revealed that the ACC structure of MeACC was derived from the L-methionine moiety of SAM, rather than L-valine. In addition, rOrf29 was found to catalyze the C-methylation of the L-methionine moiety of SAM. The resulting methylated derivative of SAM was then converted into MeACC by rOrf30. Thus, we demonstrate that C-methylation of SAM occurs prior to cyclopropanation in the biosynthesis of a bacterial MeACC (norcoronamic acid).

DOI： 10.3390/biom10050775

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Functional Characterization of 3‐Aminobenzoic Acid Adenylation Enzyme PctU and UDP‐N‐Acetyl‐D‐Glucosamine: 3‐Aminobenzoyl‐ACP Glycosyltransferase PctL in Pactamycin Biosynthesis Reviewed

Fumitaka Kudo, Jiahao Zhang, Shusuke Sato, Akane Hirayama, Tadashi Eguchi

ChemBioChem 20 ( 19 ) 2458 - 2462 2019.8

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Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley

Abstract

Pactamycin is an antibiotic produced by Streptomyces pactum with antitumor and antimalarial properties. Pactamycin has a unique aminocyclitol core that is decorated with 3‐aminoacetophenone, 6‐methylsaliciate, and an N,N‐dimethylcarbamoyl group. Herein, we show that the adenylation enzyme PctU activates 3‐aminobenzoic acid (3ABA) with adenosine triphosphate and ligates it to the holo form of the discrete acyl carrier protein PctK to yield 3ABA‐PctK. Then, 3ABA‐PctK is N‐glycosylated with uridine diphosphate‐N‐acetyl‐d‐glucosamine (UDP‐GlcNAc) by the glycosyltransferase PctL to yield GlcNAc‐3ABA‐PctK. Because 3ABA is known to be a precursor of the 3‐aminoacetophenone moiety, PctU appears to be a gatekeeper that selects the appropriate 3‐aminobenzoate starter unit. Overall, we propose that acyl carrier protein‐bound glycosylated 3ABA derivatives are biosynthetic intermediates of pactamycin biosynthesis.

DOI： 10.1002/cbic.201900239

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cbic.201900239
Biochemical and Structural Analysis of FomD That Catalyzes the Hydrolysis of Cytidylyl (S)-2-Hydroxypropylphosphonate in Fosfomycin Biosynthesis Reviewed

Shusuke Sato, Akimasa Miyanaga, Seung-Young Kim, Tomohisa Kuzuyama, Fumitaka Kudo, Tadashi Eguchi

Biochemistry 57 ( 32 ) 4858 - 4866 2018.7

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.biochem.8b00690

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Fosfomycin Biosynthesis via Transient Cytidylylation of 2-Hydroxyethylphosphonate by the Bifunctional Fom1 Enzyme Reviewed

Su-Hee Cho, Seung-Young Kim, Takeo Tomita, Taro Shiraishi, Jin-Soo Park, Shusuke Sato, Fumitaka Kudo, Tadashi Eguchi, Nobutaka Funa, Makoto Nishiyama, Tomohisa Kuzuyama

ACS Chemical Biology 12 ( 8 ) 2209 - 2215 2017.7

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Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acschembio.7b00419

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Methylcobalamin-Dependent Radical SAM C-Methyltransferase Fom3 Recognizes Cytidylyl-2-hydroxyethylphosphonate and Catalyzes the Nonstereoselective C-Methylation in Fosfomycin Biosynthesis Reviewed

Shusuke Sato, Fumitaka Kudo, Seung-Young Kim, Tomohisa Kuzuyama, Tadashi Eguchi

Biochemistry 56 ( 28 ) 3519 - 3522 2017.7

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.biochem.7b00472

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MISC

抗生物質ホスホマイシン生合成の全貌解明

佐藤秀亮, 工藤史貴, 江口正

バイオサイエンスとインダストリー 77 378 - 379 2019

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Authorship：Lead author Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal)

CiNii Research

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Other Link： https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-16H06451/

Presentations

Complete In Vitro Reconstitution of Apramycin Biosynthesis Uncovers Novel Transaldolase and Glycosyltransferase Involved in Octose-Based Pseudotrisaccharide Backbone Formation

Shusuke Sato, Po-Hsun Fan, Yu-Cheng Yeh, Hung-wen Liu

The 67th Symposium on the Chemistry of Natural Products 2025.9

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Event date： 2025.9

Language：English Presentation type：Oral presentation (general)

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バクテリオホパンポリオール生合成において炭素-炭素結合形成反応を触媒するラジカルSAM酵素HpnHの機能解析

佐藤秀亮, 工藤史貴, Michel Rohmer, 江口正

第63回天然有機化合物討論会 2021.9

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Event date： 2021.9

Language：Japanese Presentation type：Oral presentation (general)

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Radical SAM C-Methylation in Fosfomycin Biosynthesis

Shusuke Sato, Fumitaka Kudo, Tomohisa Kuzuyama, Tadashi Eguchi

The 99th CSJ Annual Meeting 2019.3

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Event date： 2019.3

Language：English Presentation type：Oral presentation (general)

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セスキテルペン環化酵素の変異導入による機能改変（１）

諏訪淳, 佐藤秀亮, 南篤志

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese

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二量体ピラノナフトキノン系抗生物質クリサミシンAの生合成機構に関する研究

笹崎潤人, 坪井一馬, 安藤吉勇, 大森建, 佐藤秀亮, 南篤志, 江口正, 工藤史貴

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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Dinemaxanthone類生合成における酸化的修飾反応の解析（１）

石塚祥太郎, 佐藤秀亮, 橋本勝, 南篤志

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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Bonnevillamide D生合成におけるアゼチジン環形成酵素の機能解析

木内光一, 長野那由多, 森脇由隆, 佐藤秀亮, 南篤志, 工藤史貴

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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放線菌由来セスキテルペン環化酵素の機構解析

原田康太, 佐藤秀亮, 池田治生, 及川英秋, 南篤志

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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分子進化の概念を考慮した多機能性巨大酵素の機能改変

松岡瑠奈, 小谷明里, 佐藤秀亮, 及川英秋, 南篤志

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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Frankia属放線菌のバクテリオホパンテトロール生合成酵素に関する研究

樋口岳紀, Michel Rohmer, 佐藤秀亮, 南篤志, 工藤史貴

日本化学会第106春季年会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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Development of Enzymatic Dimerization Towards the Chemoenzymatic Synthesis of Maleidrides

Daichi Hata, Ryo Tanifuji, Shusuke Sato, Atsushi Minami, Hiroki Oguri

The 106th CSJ Annual Meeting 2026.3

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Event date： 2026.3

Language：English Presentation type：Oral presentation (general)

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セスキテルペン環化酵素の反応制御機構に関する研究（１）

諏訪淳, 佐藤秀亮, 南篤志

日本農芸化学会2026年度京都大会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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麹菌異種発現系を用いたdinemaxanthone類の生合成研究（１）

石塚祥太郎, 佐藤秀亮, 橋本勝, 南篤志

日本農芸化学会2026年度京都大会 2026.3

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Event date： 2026.3

Language：Japanese Presentation type：Oral presentation (general)

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Novel xanthone analogues isolated from Dinemasporium parastrigosum KT4144

Kosei Kirisawa, Kazuaki Tanaka, Hayato Maeda, Shusuke Sato, Atsushi Minami, Masaru Hashimoto

The 67th Symposium on the Chemistry of Natural Products 2025.9

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Event date： 2025.9

Language：Japanese Presentation type：Oral presentation (general)

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Complete in vitro reconstitution of the apramycin biosynthetic pathway demonstrates incorporation of β-D-sugar nucleotide

Shusuke Sato, Po-Hsun Fan, Yu-Cheng Yeh, Hung-wen Liu

Discover BMB 2024 2024.3

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Event date： 2024.3

Language：English Presentation type：Oral presentation (general)

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Mechanism of the Tricyclic Undecose Core Formation in Herbicidin Biosynthesis

Shusuke Sato, Zhang Chen, Hung-wen Liu

9th Texas Enzyme Mechanisms Conference 2023.6

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Event date： 2023.6

Language：English Presentation type：Oral presentation (general)

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Functional Analysis of Radical SAM Enzymes Involved in Hopanoids Biosynthesis

Shusuke Sato, Fumitaka Kudo, Michel Rohmer, Tadashi Eguchi

Directing biosynthesis 2021.4

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Event date： 2021.4

Language：English Presentation type：Poster presentation

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バクテリオホパンポリオール生合成において炭素-炭素結合形成反応を触媒するラジカルSAM酵素HpnHの機能解析

佐藤秀亮, 工藤史貴, Michel Rohmer, 江口正

日本化学会第101春季年会 2021.3

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Event date： 2021.3

Language：Japanese Presentation type：Oral presentation (general)

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ホスホマイシン生合成における新規加水分解酵素FomDの機能解析

佐藤秀亮, 宮永顕正, 工藤史貴, 葛山智久, 江口正

日本農芸化学会 2019年度大会 2019.3

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Event date： 2019.3

Language：Japanese Presentation type：Oral presentation (general)

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C-Methylation mechanism in fosfomycin biosynthesis

Shusuke Sato, Fumitaka Kudo, Tomohisa Kuzuyama, Tadashi Eguchi

3rd European Conference on Natural Products 2018.9

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Event date： 2018.9

Language：English Presentation type：Poster presentation

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ホスホマイシン生合成におけるC-メチル化機構

佐藤秀亮, Kim Seung-Young, 葛山智久, 工藤史貴, 江口正

第59回天然有機化合物討論会 2017.9

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Event date： 2017.9

Language：Japanese Presentation type：Oral presentation (general)

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ホスホマイシン生合成におけるラジカルS-アデノシル-L-メチオニンC-メチル化酵素の機能解析

佐藤秀亮, 工藤史貴, 江口正, 葛山智久

日本化学会第97春季年会 2017.3

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Event date： 2017.3

Language：Japanese Presentation type：Oral presentation (general)

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Awards

学生講演賞

2019.3 日本化学会第９９春季年会ホスホマイシン生合成における C-メチル化機構

佐藤秀亮

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Research Projects

ラジカル反応を制御する生体触媒が織りなす天然物化学の未踏領域への挑戦

Grant number：26K17961 2026.4 - 2028.3

日本学術振興会科学研究費助成事業若手研究

佐藤秀亮

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Authorship：Principal investigator

Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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連続的シクロプロパン環導入を担うラジカルSAM酵素の精密機能解析

Grant number：25K23586 2025.7 - 2027.3

日本学術振興会科学研究費助成事業研究活動スタート支援

佐藤秀亮

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Authorship：Principal investigator

Grant amount：\2730000 （ Direct Cost: \2100000 、 Indirect Cost：\630000 ）

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Enzymatic Synthesis of Polycyclopropanated Chains

2025.7 - 2026.6

Institute of Science Tokyo ASUNARO Grant

Shusuke Sato

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Authorship：Principal investigator

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Functional analysis of radical SAM enzymes involved in carbon-carbon bond formation

Grant number：19J11692 2019.4 - 2021.3

Japan Society for the Promotion of Science Grant-in-Aid for JSPS Fellows

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Grant amount：\1900000 （ Direct Cost: \1900000 ）

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