Updated on 2025/08/15

写真a

 
MATSUURA TOMOAKI
 
Organization
Institute of Future Science Earth-Life Science Institute Professor
Title
Professor
Contact information
メールアドレス
External link

News & Topics

Research Interests

  • 実験進化学

  • タンパク質工学

  • 膜タンパク質

  • 無細胞タンパク質合成系

  • combinatorial approach

  • phage display

  • ribosome display

  • 進化分子工学

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

  • Nanotechnology/Materials / Chemical biology  / 進化分子工学

  • Life Science / Biophysics

Professional Memberships

Papers

  • Structural insights into lab-coevolved RNA–RBP pairs and applications of synthetic riboswitches in cell-free system

    Keisuke Fukunaga, Takamasa Teramoto, Momoka Nakashima, Toshitaka Ohtani, Riku Katsuki, Tomoaki Matsuura, Yohei Yokobayashi, Yoshimitsu Kakuta

    Nucleic Acids Research   53 ( 6 )   2025.3

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    CS1–LS4 and CS2–LS12 are ultra-high affinity and orthogonal RNA–protein pairs that were identified by PD-SELEX (Phage Display coupled with Systematic Evolution of Ligands by EXponential enrichment). To investigate the molecular basis of the lab-coevolved RNA–RBP pairs, we determined the structures of the CS1–LS4 and CS2–LS12 complexes and the LS12 homodimer in an RNA-free state by X-ray crystallography. The structural analyses revealed that the lab-coevolved RNA–RBPs have acquired unique molecular recognition mechanisms, whereas the overall structures of the RNP complexes were similar to the typical kink-turn RNA-L7Ae complex. The orthogonal RNA–RBP pairs were applied to construct high-performance cell-free riboswitches that regulate translation in response to LS4 or LS12. In addition, by using the orthogonal protein-responsive switches, we generated an AND logic gate that outputs staphylococcal γ-hemolysin in cell-free system and carried out hemolysis assay and calcein leakage assay using rabbit red blood cells and artificial cells, respectively.

    DOI： 10.1093/nar/gkaf212

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  • Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches. International journal

    Yuta Ishii, Keisuke Fukunaga, Aileen Cooney, Yohei Yokobayashi, Tomoaki Matsuura

    Chemical communications (Cambridge, England)   60 ( 46 )   5972 - 5975   2024.6

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    Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.

    DOI： 10.1039/d4cc00965g

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  • Lipid vesicle-based molecular robots. International journal

    Zugui Peng, Shoji Iwabuchi, Kayano Izumi, Sotaro Takiguchi, Misa Yamaji, Shoko Fujita, Harune Suzuki, Fumika Kambara, Genki Fukasawa, Aileen Cooney, Lorenzo Di Michele, Yuval Elani, Tomoaki Matsuura, Ryuji Kawano

    Lab on a chip   2024.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    A molecular robot, which is a system comprised of one or more molecular machines and computers, can execute sophisticated tasks in many fields that span from nanomedicine to green nanotechnology. The core parts of molecular robots are fairly consistent from system to system and always include (i) a body to encapsulate molecular machines, (ii) sensors to capture signals, (iii) computers to make decisions, and (iv) actuators to perform tasks. This review aims to provide an overview of approaches and considerations to develop molecular robots. We first introduce the basic technologies required for constructing the core parts of molecular robots, describe the recent progress towards achieving higher functionality, and subsequently discuss the current challenges and outlook. We also highlight the applications of molecular robots in sensing biomarkers, signal communications with living cells, and conversion of energy. Although molecular robots are still in their infancy, they will unquestionably initiate massive change in biomedical and environmental technology in the not too distant future.

    DOI： 10.1039/d3lc00860f

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  • Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production

    Ryota Ushiyama, Satoshi Nanjo, Mamiko Tsugane, Reiko Sato, Tomoaki Matsuura, Hiroaki Suzuki

    ACS Synthetic Biology   13 ( 1 )   68 - 76   2023.11

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    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acssynbio.3c00629

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  • Simple Method for the Creation of a Bacteria-Sized Unilamellar Liposome with Different Proteins Localized to the Respective Sides of the Membrane

    Kosaku Noba, Shogo Yoshimoto, Yoshikazu Tanaka, Takeshi Yokoyama, Tomoaki Matsuura, Katsutoshi Hori

    ACS Synthetic Biology   2023.5

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    Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acssynbio.2c00564

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  • ePURE_JSBML: A Tool for Constructing a Deterministic Model of a Reconstituted Escherichia coli Protein Translation System with a User‐Specified Nucleic Acid Sequence Reviewed

    Yoshihiro Shimizu, Naoki Tanimura, Tomoaki Matsuura

    Advanced Biology   2023.3

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/adbi.202200177

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  • A methodology for creating thermostabilized mutants of G‐protein coupled receptors by combining statistical thermodynamics and evolutionary molecular engineering

    Kanna Sugaya, Satoshi Yasuda, Shingo Sato, Chen Sisi, Taisei Yamamoto, Daisuke Umeno, Tomoaki Matsuura, Tomohiko Hayashi, Satoshi Ogasawara, Masahiro Kinoshita, Takeshi Murata

    Protein Science   31 ( 9 )   2022.9

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/pro.4404

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pro.4404

  • Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display. International journal

    Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe, Tomoaki Matsuura

    Analytical chemistry   94 ( 9 )   3831 - 3839   2022.2

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    Engineering G-protein-coupled receptors (GPCRs) for improved stability or altered function is of great interest, as GPCRs consist of the largest protein family, are involved in many important signaling pathways, and thus, are one of the major drug targets. Here, we report the development of a high-throughput screening method for GPCRs using a reconstituted in vitro transcription-translation (IVTT) system. Human endothelin receptor type-B (ETBR), a class A GPCR that binds endothelin-1 (ET-1), a 21-residue peptide hormone, was synthesized in the presence of nanodisc (ND) composed of a phospholipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG). The ET-1 binding of ETBR was significantly reduced or was undetectable when other phospholipids were used for ND preparation. However, when functional ETBR purified from Sf9 cells was reconstituted into NDs, ET-1 binding was observed with two different phospholipids tested, including POPG. These results suggest that POPG likely supports the folding of ETBR into its functional form in the IVTT system. Using the same conditions as ETBR, whose three-dimensional structure has been solved, human endothelin receptor type-A (ETAR), whose three-dimensional structure remains unsolved, was also synthesized in its functional form. By adding POPG-ND to the IVTT system, both ETAR and ETBR were successfully subjected to ribosome display, a method of in vitro directed evolution that facilitates the screening of up to 1012 mutants. Finally, using a mock library, we showed that ribosome display can be applied for gene screening of ETBR, suggesting that high-throughput screening and directed evolution of GPCRs is possible in vitro.

    DOI： 10.1021/acs.analchem.1c04714

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  • Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression. International journal

    Christelle Alexa Garcia Perez, Shungo Adachi, Quang Dang Nong, Nikko Adhitama, Tomoaki Matsuura, Toru Natsume, Tadashi Wada, Yasuhiko Kato, Hajime Watanabe

    PLoS genetics   17 ( 7 )   e1009683   2021.7

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    Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed the lncRNA DAPALR overlapping with the 5´ UTR of the Doublesex1 (Dsx1), the male determining gene in Daphnia magna. By affinity purification, we identified an RNA binding protein, Shep as a DAPALR binding protein. Shep also binds to Dsx1 5´ UTR by recognizing the overlapping sequence and suppresses translation of the mRNA. In vitro and in vivo analyses indicated that DAPALR increased Dsx1 translation efficiency by sequestration of Shep. This regulation was impaired when the Shep binding site in DAPALR was deleted. These results suggest that Shep suppresses the unintentional translation of Dsx1 by setting a threshold; and when the sense lncRNA DAPALR is expressed, DAPALR cancels the suppression caused by Shep. This mechanism may be important to show dimorphic gene expressions such as sex determination and it may account for the binary expression in various developmental processes.

    DOI： 10.1371/journal.pgen.1009683

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  • Mutation of the Cytochrome P450 CYP360A8 Gene Increases Sensitivity to Paraquat in Daphnia magna. International journal

    Pijar Religia, Nhan Duc Nguyen, Quang Dang Nong, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Environmental toxicology and chemistry   40 ( 5 )   1279 - 1288   2021.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    The freshwater crustacean Daphnia magna has traditionally been a model for ecotoxicological studies owing to its sensitivity to many xenobiotics. Because it is used in many toxicity assessments, its detoxification mechanism for xenobiotics is important and requires further study. However, studies related to detoxification genes are limited to transcriptomic profiling, and there are no D. magna mutants for use in the understanding of xenobiotic metabolism in vivo. We report the generation of a D. magna CYP360A8 mutant-the gene is a cytochrome P450 (CYP) clan 3 gene. Based on RNA sequencing of adult D. magna, we found that CYP360A8 has the highest expression level among all CYP genes. At ovarian maturation, its expression level is up-regulated 6-fold compared to the juvenile stages and is maintained thereafter. Using the CRISPR/CRISPR-associated 9 (Cas9) system, we disrupted CYP360A8 by coinjecting CYP360A8-targeting guide RNA and Cas9 proteins into D. magna eggs and established one monoallelic CYP360A8 mutant line. This CYP360A8 mutant had a higher sensitivity to the herbicide paraquat compared to the wild type. We confirmed the up-regulation of CYP360A8 by paraquat. The results demonstrate the role of CYP360A8 in paraquat detoxification. The present study establishes a CYP mutant of D. magna, and this strategy can be a basic platform to document a range of CYP gene-xenobiotic relationships in this species. Environ Toxicol Chem 2021;40:1279-1288. © 2020 SETAC.

    DOI： 10.1002/etc.4970

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  • DNMT3.1 controls trade-offs between growth, reproduction, and life span under starved conditions in Daphnia magna. International journal

    Nhan Duc Nguyen, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Scientific reports   11 ( 1 )   7326 - 7326   2021.4

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    The cladoceran crustacean Daphnia has long been a model of energy allocation studies due to its important position in the trophic cascade of freshwater ecosystems. However, the loci for controlling energy allocation between life history traits still remain unknown. Here, we report CRISPR/Cas-mediated target mutagenesis of DNA methyltransferase 3.1 (DNMT3.1) that is upregulated in response to caloric restriction in Daphnia magna. The resulting biallelic mutant is viable and did not show any change in growth rate, reproduction, and longevity under nutrient rich conditions. In contrast, under starved conditions, the growth rate of this DNMT3.1 mutant was increased but its reproduction was reciprocally reduced compared to the wild type when the growth and reproduction activities competed during a period from instar 4 to 8. The life span of this mutant was significantly shorter than that of the wild type. We also compared transcriptomes between DNMT3.1 mutant and wild type under nutrient-rich and starved conditions. Consistent with the DNMT3.1 mutant phenotypes, the starved condition led to changes in the transcriptomes of the mutant including differential expression of vitellogenin genes. In addition, we found upregulation of the I am not dead yet (INDY) ortholog, which has been known to shorten the life span in Drosophila, explaining the shorter life span of the DNMT3.1 mutant. These results establish DNMT3.1 as a key regulator for life span and energy allocation between growth and reproduction during caloric restriction. Our findings reveal how energy allocation is implemented by selective expression of a DNMT3 ortholog that is widely distributed among animals. We also infer a previously unidentified adaptation of Daphnia that invests more energy for reproduction than growth under starved conditions.

    DOI： 10.1038/s41598-021-86578-4

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  • Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant. International journal

    Nur Izzatur Binti Ismail, Yasuhiko Kato, Tomoaki Matsuura, Cristian Gómez-Canela, Carlos Barata, Hajime Watanabe

    Genesis (New York, N.Y. : 2000)   59 ( 3 )   e23403   2021.3

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    The ABC transporter, Scarlet, and its binding partner, White are involved in pigment synthesis in the insect eye and mutations in these genes are used as genetic markers. Recent studies have suggested that these transporters also have additional functions in the neuronal system. In our previous study, we generated scarlet mutant in the small crustacean, Daphnia magna and showed that the mutant lacked the eye pigment in the mutant. Here, we show that the scarlet mutant exhibits spinning behavior. This phenotype is partly associated with the presence of light. Metabolomic analysis of a juvenile mutant revealed that the scarlet mutant has approximately one-tenth of the histamine content of the wild type. Application of histamine to the scarlet mutant rescued the spinning behavior in juveniles, suggesting that the spinning behavior of the mutant is caused by the reduction of histamine. However, the altered behavior was not rescued in the adult mutant by the addition of histamine, suggesting that Scarlet plays an irreversible role in the development of histaminergic neurons. These results suggest that Scarlet plays an important role in histaminergic signaling, which might be related to control the spinning behavior, in addition to its role in eye pigmentation.

    DOI： 10.1002/dvg.23403

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  • Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system

    Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori, Tomoaki Matsuura

    Journal of Bioscience and Bioengineering   2021

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier B.V.  

    DOI： 10.1016/j.jbiosc.2021.10.008

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  • Production of genome-edited Daphnia for heavy metal detection by fluorescence. International journal

    Takuto Arao, Yasuhiko Kato, Quang Dang Nong, Hiroshi Yamamoto, Haruna Watanabe, Tomoaki Matsuura, Norihisa Tatarazako, Kazune Tani, Akira Okamoto, Takeru Matsumoto, Hajime Watanabe

    Scientific reports   10 ( 1 )   21490 - 21490   2020.12

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    Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from Daphnia magna. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into D. magna using the TALEN technique and transgenic Daphnia named D. magna MetalloG were produced. When D. magna MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn2+, 130 nM Cu2+, and 70 nM Cd2+. Heavy metal exposure for 24 h could lower the thresholds even further. D. magna MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.

    DOI： 10.1038/s41598-020-78572-z

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  • Caloric restriction upregulates the expression of DNMT3.1, lacking the conserved catalytic domain, in Daphnia magna. International journal

    Nhan Duc Nguyen, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Genesis (New York, N.Y. : 2000)   58 ( 12 )   e23396   2020.12

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    DNA methylation plays an important role in many aspects of biology, including development, disease, and phenotypic plasticity. In the branchiopod crustacean, Daphnia, de novo DNA methylation has been detected in specific environmental contexts. However, fundamental information on de novo DNA methyltransferase DNMT3 orthologs, including domain organization, developmental expression, and response to environmental stimuli, is lacking. In this study, we examined two DNMT3 orthologs in Daphnia magna, DapmaDNMT3.1 and DapmaDNMT3.2. Amino acid sequence alignment revealed that DapmaDNMT3.1 and DapmaDNMT3.2 lack the conserved methyltransferase motifs of the catalytic domain and the PWWP domain, respectively. We profiled the expression of the two orthologs during embryogenesis and under various feeding levels. During embryogenesis, in contrast to the low DapmaDNMT3.1 expression, DapmaDNTM3.2 was highly expressed at specific stages, that is, in the one cell-stage and at 48 hr post ovulation. In nutrient-rich condition, both genes were lowly expressed, whereas DapmaDNMT3.1 was upregulated at the lower food levels, suggesting a potential role of DapmaDNMT3.1 in gene regulation in response to caloric restriction. These findings provide a basis for understanding the developmental stage- and stress-dependent function of DNMT3 orthologs in D. magna.

    DOI： 10.1002/dvg.23396

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  • Computational design of transmembrane pores. Reviewed International journal

    Chunfu Xu, Peilong Lu, Tamer M Gamal El-Din, Xue Y Pei, Matthew C Johnson, Atsuko Uyeda, Matthew J Bick, Qi Xu, Daohua Jiang, Hua Bai, Gabriella Reggiano, Yang Hsia, T J Brunette, Jiayi Dou, Dan Ma, Eric M Lynch, Scott E Boyken, Po-Ssu Huang, Lance Stewart, Frank DiMaio, Justin M Kollman, Ben F Luisi, Tomoaki Matsuura, William A Catterall, David Baker

    Nature   585 ( 7823 )   129 - 134   2020.9

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    Transmembrane channels and pores have key roles in fundamental biological processes1 and in biotechnological applications such as DNA nanopore sequencing2-4, resulting in considerable interest in the design of pore-containing proteins. Synthetic amphiphilic peptides have been found to form ion channels5,6, and there have been recent advances in de novo membrane protein design7,8 and in redesigning naturally occurring channel-containing proteins9,10. However, the de novo design of stable, well-defined transmembrane protein pores that are capable of conducting ions selectively or are large enough to enable the passage of small-molecule fluorophores remains an outstanding challenge11,12. Here we report the computational design of protein pores formed by two concentric rings of α-helices that are stable and monodisperse in both their water-soluble and their transmembrane forms. Crystal structures of the water-soluble forms of a 12-helical pore and a 16-helical pore closely match the computational design models. Patch-clamp electrophysiology experiments show that, when expressed in insect cells, the transmembrane form of the 12-helix pore enables the passage of ions across the membrane with high selectivity for potassium over sodium; ion passage is blocked by specific chemical modification at the pore entrance. When incorporated into liposomes using in vitro protein synthesis, the transmembrane form of the 16-helix pore-but not the 12-helix pore-enables the passage of biotinylated Alexa Fluor 488. A cryo-electron microscopy structure of the 16-helix transmembrane pore closely matches the design model. The ability to produce structurally and functionally well-defined transmembrane pores opens the door to the creation of designer channels and pores for a wide variety of applications.

    DOI： 10.1038/s41586-020-2646-5

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  • Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna. Reviewed International journal

    Quang Dang Nong, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    PloS one   15 ( 8 )   e0238256   2020

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    In recent years, the binary definition of sex is being challenged by repetitive reports about individuals with ambiguous sexual identity from various animal groups. This has created an urge to decode the molecular mechanism underlying sexual development. However, sexual ambiguities are extremely uncommon in nature, limiting their experimental value. Here, we report the establishment of a genetically modified clone of Daphnia magna from which intersex daphniids can be readily generated. By mutating the conserved central sex determining factor Doublesex1, body-wide feminization of male daphniid could be achieved. Comparative transcriptomic analysis also revealed a genetic network correlated with Doublesex1 activity which may account for the establishment of sexual identity in D. magna. We found that Dsx1 repressed genes related to growth and promoted genes related to signaling. We infer that different intersex phenotypes are the results of fluctuation in activity of these Dsx1 downstream factors. Our results demonstrated that the D. magna genome is capable of expressing sex in a continuous array, supporting the idea that sex is actually a spectrum.

    DOI： 10.1371/journal.pone.0238256

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  • Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna. Reviewed International journal

    Nikko Adhitama, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    PloS one   15 ( 10 )   e0239893   2020

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    The ecdysteroid and sesquiterpenoid pathways control growth, developmental transition, and embryogenesis in insects. However, the function of orthologous genes and the cross-talk between both pathways remain largely uncharacterized in non-insect arthropods. Spook (Spo) and Juvenile hormone acid o-methyltransferase (Jhamt) have been suggested to function as rate-limiting factors in ecdysteroid and sesquiterpenoid biosynthesis, respectively, in insects. In this study, we report on the functions of Spo and Jhamt and the cross-talk between them in embryos of the branchiopod crustacean Daphnia magna. Spo expression was activated at the onset of gastrulation, with the depletion of Spo transcript by RNAi resulting in developmental arrest at this stage. This phenotype could be partially rescued by supplementation with 20-hydroxyecdysone, indicating that Spo may play the same role in ecdysteroid biosynthesis in early embryos, as reported in insects. After hatching, Spo expression was repressed, while Jhamt expression was activated transiently, despite its silencing during other embryonic stages. Jhamt RNAi showed little effect on survival, but shortened the embryonic period. Exposure to the sesquiterpenoid analog Fenoxycarb extended the embryonic period and rescued the Jhamt RNAi phenotype, demonstrating a previously unidentified role of sesquiterpenoid in the repression of precocious embryogenesis. Interestingly, the knockdown of Jhamt resulted in the derepression of ecdysteroid biosynthesis genes, including Spo, similar to regulation during insect hormonal biosynthesis. Sesquiterpenoid signaling via the Methoprene-tolerant gene was found to be responsible for the repression of ecdysteroid biosynthesis genes. It upregulated an ortholog of CYP18a1 that degrades ecdysteroid in insects. These results illuminate the conserved and specific functions of the ecdysteroid and sesquiterpenoid pathways in Daphnia embryos. We also infer that the common ancestor of branchiopod crustaceans and insects exhibited antagonism between the two endocrine hormones before their divergence 400 million years ago.

    DOI： 10.1371/journal.pone.0239893

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  • Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA. Reviewed International journal

    Kosaku Noba, Masahito Ishikawa, Atsuko Uyeda, Takayoshi Watanabe, Takahiro Hohsaka, Shogo Yoshimoto, Tomoaki Matsuura, Katsutoshi Hori

    Journal of the American Chemical Society   141 ( 48 )   19058 - 19066   2019.12

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    The bacterial cell surface structure has important roles for various cellular functions. However, research on reconstituting bacterial cell surface structures is limited. This study aimed to bottom-up create a cell-sized liposome covered with AtaA, the adhesive bacterionanofiber protein localized on the cell surface of Acinetobacter sp. Tol 5, without the use of the protein secretion and assembly machineries. Liposomes containing a benzylguanine derivative-modified phospholipid were decorated with a truncated AtaA protein fused to a SNAP-tag expressed in a soluble fraction in Escherichia coli. The obtained liposome showed a similar surface structure and function to that of native Tol 5 cells and adhered to both hydrophobic and hydrophilic solid surfaces. Furthermore, this artificial cell was able to drive an enzymatic reaction in the adhesive state. The developed artificial cellular system will allow for analysis of not only AtaA, but also other cell surface proteins under a cell-mimicking environment. In addition, AtaA-decorated artificial cells may inspire the development of biotechnological applications that require immobilization of cells onto a variety of solid surfaces, in particular, in environments where the use of genetically modified organisms is prohibited.

    DOI： 10.1021/jacs.9b09340

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  • Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine-5'-Monophosphate. Reviewed International journal

    Marin Ogawa, Atsuko Uyeda, Kazuo Harada, Yu Sato, Yasuhiko Kato, Hajime Watanabe, Kohsuke Honda, Tomoaki Matsuura

    Chembiochem : a European journal of chemical biology   20 ( 23 )   2961 - 2967   2019.12

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    Polyphosphate kinase 2 (PPK2) transfer phosphate from inorganic polyphosphate to nucleotides. According to their activity, PPK2 enzymes are classified into three groups. Among them, class III enzymes catalyze both the phosphorylation of nucleotide mono- to diphosphates and di- to triphosphates by using polyphosphate, which is a very inexpensive substrate. Therefore, class III enzymes are very attractive for use in biotechnological applications. Despite several studies on class III enzymes, a detailed mechanism of how phosphate is transferred from the polyphosphate to the nucleotide remains to be elucidated. Herein, it is reported that PPK2 class III enzymes from two different bacterial species catalyze the phosphorylation of adenosine mono- (AMP) into triphosphate (ATP) not only through step-by-step phosphorylation, but also by pyrophosphorylation. These are the first PPK2 enzymes that have been shown to possess polyphosphate-dependent pyrophosphorylation activity.

    DOI： 10.1002/cbic.201900303

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  • Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch. Reviewed International journal

    Mohammed Dwidar, Yusuke Seike, Shungo Kobori, Charles Whitaker, Tomoaki Matsuura, Yohei Yokobayashi

    Journal of the American Chemical Society   141 ( 28 )   11103 - 11114   2019.7

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    Artificial cells that encapsulate DNA-programmable protein expression machinery are emerging as an attractive platform for studying fundamental cellular properties and applications in synthetic biology. However, interfacing these artificial cells with the complex and dynamic chemical environment remains a major and urgent challenge. We demonstrate that the repertoire of molecules that artificial cells respond to can be expanded by synthetic RNA-based gene switches, or riboswitches. We isolated an RNA aptamer that binds histamine with high affinity and specificity and used it to design robust riboswitches that activate protein expression in the presence of histamine. Finally, the riboswitches were incorporated in artificial cells to achieve controlled release of an encapsulated small molecule and to implement a self-destructive kill-switch. Synthetic riboswitches should serve as modular and versatile interfaces to link artificial cell phenotypes with the complex chemical environment.

    DOI： 10.1021/jacs.9b03300

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  • In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency. Reviewed

    Kosuke Okamura, Shuhei Matsushita, Yasuhiko Kato, Hajime Watanabe, Aiko Matsui, Toshihiko Oka, Tomoaki Matsuura

    Journal of bioscience and bioengineering   127 ( 5 )   544 - 548   2019.5

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    The human mitochondrion-derived calcium transporter Letm1 was synthesized by reconstituted in vitro transcription-translation (IVTT) in cell-sized liposomes and the dependency of Letm1 on phospholipid composition was investigated. Components for IVTT were encapsulated into cell-sized vesicles together with the DNA encoding Letm1, thereby preparing proteoliposomes. The synthesis of Letm1 and pH-dependent calcium transport activity were confirmed by flow cytometry. Finally, we investigated the effect of phospholipid composition on Letm1 transport activity and found that cardiolipin present in the mitochondrial membrane plays an important role on the transport activity of Letm1.

    DOI： 10.1016/j.jbiosc.2018.11.003

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  • Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna. Reviewed International journal

    Pijar Religia, Yasuhiko Kato, Ery Odette Fukushima, Tomoaki Matsuura, Toshiya Muranaka, Hajime Watanabe

    Marine environmental research   145   177 - 183   2019.3

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    This study focuses on the possibility that herbicide-exposed phytoplankton will cause sub-lethal effect on zooplankton. Atrazine, phytoplankton Raphidocelis subcapitata and zooplankton Daphnia magna were chosen as a model chemical and organisms. R. subcapitata was exposed to atrazine at 150 μg/L, harvested and fed to D. magna. While the mothers fed with atrazine-exposed phytoplankton did not show any abnormalities, they produced non-viable offspring. Number of non-viable offspring at the first clutch was high but the number was reduced at later stages and viable offspring was produced. This result indicates that phytoplankton exposed to sub-lethal dose of atrazine affects population dynamics of its predator, D. magna.

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  • Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer. Reviewed International journal

    Maie A Elfaramawy, Satoshi Fujii, Atsuko Uyeda, Toshihisa Osaki, Shoji Takeuchi, Yasuhiko Kato, Hajime Watanabe, Tomoaki Matsuura

    Chemical communications (Cambridge, England)   54 ( 86 )   12226 - 12229   2018.10

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    DOI： 10.1039/c8cc06804f

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  • Monitoring ecdysteroid activities using genetically encoded reporter gene in Daphnia magna. Reviewed International journal

    Nikko Adhitama, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Marine environmental research   140   375 - 381   2018.9

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    DOI： 10.1016/j.marenvres.2018.07.003

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  • Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational Modeling. Reviewed International journal

    Tomoaki Matsuura, Kazufumi Hosoda, Yoshihiro Shimizu

    ACS synthetic biology   7 ( 8 )   1964 - 1972   2018.8

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    DOI： 10.1021/acssynbio.8b00228

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  • A 5' UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna. Reviewed International journal

    Yasuhiko Kato, Christelle Alexa G Perez, Nur Syafiqah Mohamad Ishak, Quang D Nong, Yuumi Sudo, Tomoaki Matsuura, Tadashi Wada, Hajime Watanabe

    Current biology : CB   28 ( 11 )   1811 - 1817   2018.6

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    DOI： 10.1016/j.cub.2018.04.029

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  • Concurrent In Vitro Synthesis and Functional Detection of Nascent Activity of the KcsA Channel under a Membrane Potential. Reviewed International journal

    Masayuki Iwamoto, Maie A Elfaramawy, Mariko Yamatake, Tomoaki Matsuura, Shigetoshi Oiki

    ACS synthetic biology   7 ( 4 )   1004 - 1011   2018.4

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    DOI： 10.1021/acssynbio.7b00454

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  • Generation of white-eyed Daphnia magna mutants lacking scarlet function. Reviewed International journal

    Nur Izzatur Binti Ismail, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    PloS one   13 ( 11 )   e0205609   2018

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    DOI： 10.1371/journal.pone.0205609

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  • Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna. Reviewed International journal

    Kerstin Törner, Tsuyoshi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    PloS one   13 ( 6 )   e0198023   2018

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    DOI： 10.1371/journal.pone.0198023

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  • Different protein localizations on the inner and outer leaflet of cell-sized liposomes using cell-free protein synthesis. Reviewed International journal

    Atsuko Uyeda, Takayoshi Watanabe, Takahiro Hohsaka, Tomoaki Matsuura

    Synthetic biology (Oxford, England)   3 ( 1 )   ysy007   2018

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    Membranes of living cells possess asymmetry. The inner and outer leaflets of the membrane consist of different phospholipid compositions, which are known to affect the function of membrane proteins, and the loss of the asymmetry has been reported to lead to cell apoptosis. In addition, different proteins are found on the inner and outer leaflets of the membrane, and they are essential for various biochemical reactions, including those related to signal transduction and cell morphology. While in vitro lipid bilayer reconstitution with asymmetric phospholipid compositions has been reported, the reconstitution of lipid bilayer where different proteins are localized in the inner and outer leaflet, thereby enables asymmetric protein localizations, has remained difficult. Herein, we developed a simple method to achieve this asymmetry using an in vitro transcription-translation system (IVTT). The method used a benzylguanine (BG) derivative-modified phospholipid, which forms a covalent bond with a snap-tag sequence. We show that purified snap-tagged protein can be localized to the cell-sized liposome surface via an interaction between BG and the snap-tag. We then show that IVTT-synthesized proteins can be located at the lipid membrane and that different proteins can be asymmetrically localized on the outer and inner leaflets of liposomes.

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  • Development of a bicistronic expression system in the branchiopod crustacean Daphnia magna. Reviewed International journal

    Hitoshi Kumagai, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Genesis (New York, N.Y. : 2000)   55 ( 12 )   2017.12

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    DOI： 10.1002/dvg.23083

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  • Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter. Reviewed International journal

    Quang Dang Nong, Nur Syafiqah Mohamad Ishak, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    Scientific reports   7 ( 1 )   13521 - 13521   2017.11

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    DOI： 10.1038/s41598-017-13730-4

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  • Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna Reviewed

    Nur Syafiqah Mohamad Ishak, Quang Dang Nong, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    PLOS GENETICS   13 ( 11 )   2017.11

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    DOI： 10.1371/journal.pgen.1006953

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  • Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna. Reviewed International journal

    Nur Syafiqah Mohamad Ishak, Quang Dang Nong, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    PLoS genetics   13 ( 11 )   e1006953   2017.11

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  • Effects of symbiotic bacteria on chemical sensitivity of Daphnia magna Reviewed

    Patcharaporn Manakul, Saranya Peerakietkhajor, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    MARINE ENVIRONMENTAL RESEARCH   128   70 - 75   2017.7

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    DOI： 10.1016/j.marenvres.2017.03.001

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  • Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling Reviewed

    Tomoaki Matsuura, Naoki Tanimura, Kazufumi Hosoda, Tetsuya Yomo, Yoshihiro Shimizu

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 8 )   E1336 - E1344   2017.2

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    DOI： 10.1073/pnas.1615351114

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  • CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna. Reviewed International journal

    Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    PloS one   12 ( 10 )   e0186112   2017

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  • Construction of an in Vitro Gene Screening System of the E. coli EmrE Transporter Using Liposome Display Reviewed

    Atsuko Uyeda, Shintaro Nakayama, Yasuhiko Kato, Hajime Watanabe, Tomoaki Matsuura

    ANALYTICAL CHEMISTRY   88 ( 24 )   12028 - 12035   2016.12

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    DOI： 10.1021/acs.analchem.6b02308

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  • TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna Reviewed

    Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    SCIENTIFIC REPORTS   6   36252   2016.11

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    DOI： 10.1038/srep36252

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  • In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes Reviewed

    Naoki Ohta, Yasuhiko Kato, Hajime Watanabe, Hirotada Mori, Tomoaki Matsuura

    SCIENTIFIC REPORTS   6   36466   2016.11

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  • Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem Reviewed

    Saranya Peerakietkhajorn, Yasuhiko Kato, Vojtech Kasalicky, Tomoaki Matsuura, Hajime Watanabe

    ENVIRONMENTAL MICROBIOLOGY   18 ( 8 )   2366 - 2374   2016.8

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    DOI： 10.1111/1462-2920.12919

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  • Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna Reviewed

    Nur Syafiqah Mohamad Ishak, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    PLOS ONE   11 ( 5 )   e0154636   2016.5

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  • TALEN-mediated homologous recombination in Daphnia magna Reviewed

    Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    SCIENTIFIC REPORTS   5   18312   2015.12

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    DOI： 10.1038/srep18312

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  • Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation Reviewed

    Atsuko Uyeda, Takayoshi Watanabe, Yasuhiko Kato, Hajime Watanabe, Tetsuya Yomo, Takahiro Hohsaka, Tomoaki Matsuura

    CHEMBIOCHEM   16 ( 12 )   1797 - 1802   2015.8

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    DOI： 10.1002/cbic.201500174

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  • Membrane Curvature Affects the Formation of a-Hemolysin Nanopores Reviewed

    Satoshi Fujii, Tornoald Matsuura, Tetsuya Yomo

    ACS CHEMICAL BIOLOGY   10 ( 7 )   1694 - 1701   2015.7

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    DOI： 10.1021/acschembio.5b00107

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  • Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna Reviewed

    Saranya Peerakietkhajorn, Koji Tsukada, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    ENVIRONMENTAL MICROBIOLOGY REPORTS   7 ( 2 )   364 - 372   2015.4

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    DOI： 10.1111/1758-2229.12260

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  • Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna Reviewed

    Akiko Naitou, Yasuhiko Kato, Takashi Nakanishi, Tomoaki Matsuura, Hajime Watanabe

    BIOLOGY OPEN   4 ( 3 )   364 - 369   2015.3

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    DOI： 10.1242/bio.20149738

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  • Growth evaluation method by live imaging of Daphnia magna and its application to the estimation of an insect growth regulator Reviewed

    Akiko Suzuki, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    JOURNAL OF APPLIED TOXICOLOGY   35 ( 1 )   68 - 74   2015.1

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    DOI： 10.1002/jat.2988

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  • In vitro directed evolution of alpha-hemolysin by liposome display Reviewed

    Satoshi Fujii, Tomoaki Matsuura, Tetsuya Yomo

    Biophysics (Japan)   11   67 - 72   2015

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    DOI： 10.2142/biophysics.11.67

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  • Effects of symbiotic bacteria on the chemical toxicity test using Daphnia magna

    MANAKUL Patcaraporn, SARANYA Peerakietkhajorn, MATSUURA Tomoaki, KATO Yasuhiko, WATANABE Hajime

    Annual Meeting of the Japanese Society of Toxicology   42   O - 37   2015

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    The crustacean zooplankton Daphnia magna is highly sensitive to chemicals and it has been used for chemical toxicity tests according to the OECD guidelines. Recently we identified symbiotic bacteria in Daphnia magna, which plays critical roles on the growth and the reproduction. To evaluate the effects of symbiotic bacteria on the chemical sensitivity of Daphnia, bacteria-free Daphnia were prepared and chemical sensitivities were compared between bacteria-free Daphnia and normal Daphnia (with symbiotic bacteria) under the acute immobilization test. <br>Firstly we developed a method for preparation of bacteria-free Daphnia. The elimination of symbiotic bacteria was confirmed by PCR targeted to bacterial 16S ribosomal DNA. Then acute immobilization test was performed using the bacteria-free Daphnia. Normal Daphnia showed greater chemical resistance to higher concentration of Nonylphenol, Fenoxycarb and Pentachlorophenol than bacteria-free Daphnia. Interestingly, toxicity of antimicirobial reagent Triclosan did not show significant difference between normal and bacteria-free Daphnia. <br>These results suggested potential roles of symbiotic bacteria in relation to the chemical resistance of its host Daphnia. Thus it is possible that multiple contaminant including antibiotics may rise the sensitivity of Daphnia to chemicals and that the differences of toxicity data on Daphnia among laboratories may partly depend on the composition of symbiotic bacteria.

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  • Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system Reviewed

    Yasuaki Kazuta, Tomoaki Matsuura, Norikazu Ichihashi, Tetsuya Yomo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   118 ( 5 )   554 - 557   2014.11

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    DOI： 10.1016/j.jbiosc.2014.04.019

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  • The Evolutionary Enhancement of Genotype-Phenotype Linkages in the Presence of Multiple Copies of Genetic Material Reviewed

    Keisuke Uno, Takeshi Sunami, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo

    CHEMBIOCHEM   15 ( 15 )   2281 - 2288   2014.10

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    DOI： 10.1002/cbic.201402299

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  • Optimization of mRNA design for protein expression in the crustacean Daphnia magna Reviewed

    Kerstin Toerner, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

    MOLECULAR GENETICS AND GENOMICS   289 ( 4 )   707 - 715   2014.8

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    DOI： 10.1007/s00438-014-0830-8

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  • In Vitro Selection And Evolution Of Membrane Proteins Using Liposome Display Reviewed

    Tomoaki Matsuura, Satoshi Fujii, Yasuaki Kazuta, Takeshi Sunami, Tetsuya Yomo

    PROTEIN SCIENCE   23   154 - 155   2014.7

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  • Liposome display for in vitro selection and evolution of membrane proteins Reviewed

    Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Takehiro Nishikawa, Yasuaki Kazuta, Tetsuya Yomo

    NATURE PROTOCOLS   9 ( 7 )   1578 - 1591   2014.7

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    DOI： 10.1038/nprot.2014.107

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  • Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions Reviewed

    Taiji Okano, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    ACS SYNTHETIC BIOLOGY   3 ( 6 )   347 - 352   2014.6

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    DOI： 10.1021/sb400087e

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  • In Vitro Membrane Protein Synthesis Inside Cell-Sized Vesicles Reveals the Dependence of Membrane Protein Integration on Vesicle Volume Reviewed

    Haruka Soga, Satoshi Fujii, Tetsuya Yomo, Yasuhiko Kato, Hajime Watanabe, Tomoaki Matsuura

    ACS SYNTHETIC BIOLOGY   3 ( 6 )   372 - 379   2014.6

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    DOI： 10.1021/sb400094c

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  • CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna Reviewed

    Takashi Nakanishi, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    PLOS ONE   9 ( 5 )   e98363   2014.5

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    DOI： 10.1371/journal.pone.0098363

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  • In vitro evolution of membrane protein, alpha-hemolysin using a liposome display Reviewed

    Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta, Tetsuya Yomo

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   247   2014.3

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  • Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia Reviewed

    Miki Asada, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    MARINE ENVIRONMENTAL RESEARCH   93   118 - 122   2014.2

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    DOI： 10.1016/j.marenvres.2013.11.005

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  • Effects of ribosomes on the kinetics of Q beta replication Reviewed

    Kimihito Usui, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomoa

    FEBS LETTERS   588 ( 1 )   117 - 123   2014.1

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    DOI： 10.1016/j.febslet.2013.11.018

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  • Early Embryonic Expression of a Putative Ecdysteroid-Phosphate Phosphatase in the Water Flea, Daphnia magna (Cladocera: Daphniidae) Reviewed

    Miki Asada, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    JOURNAL OF INSECT SCIENCE   14   181   2014.1

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    DOI： 10.1093/jisesa/ieu043

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  • 2P089 Directed evolution of membrane protein, alpha hemolysin, by development of liposome display method(01F. Protein: Engineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Fujii Satoshi, Matsuura Tomoaki, Sunami Takeshi, Kazuta Yasuaki, Yomo Tetsuya

    Seibutsu Butsuri   54 ( 1 )   S209   2014

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    DOI： 10.2142/biophys.54.S209_5

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  • Identification of giant unilamellar vesicles with permeability to small charged molecules Reviewed

    Koji Nishimura, Tomoaki Matsuura, Takeshi Sunami, Satoshi Fujii, Kazuya Nishimura, Hiroaki Suzuki, Tetsuya Yomo

    RSC ADVANCES   4 ( 66 )   35224 - 35232   2014

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    DOI： 10.1039/c4ra05332j

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  • Liposome Display: In Vitro Directed Evolution of Membrane Proteins Reviewed

    FUJII Satoshi, MATSUURA Tomoaki, YOMO Tetsuya

    Seibutsu Butsuri   54 ( 3 )   146 - 149   2014

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    Although several methods of &lt;i&gt;in vitro&lt;/i&gt; directed evolution have been developed, they were applied to globular proteins, but not to membrane proteins. Liposome display is a novel method, which enables the directed evolution of membrane protein &lt;i&gt;in vitro&lt;/i&gt;. Membrane protein was synthesized by the cell-free translation system in a cell-sized liposome, and its function was probed by a fluorescence indicator. Liposome with high fluorescence intensity was selected by a cell sorter, enabling the isolation of DNA encoding the evolved protein. Here, we show the application of this method to α-hemolysin, one of the membrane proteins from &lt;i&gt;Staphylococcus aureus&lt;/i&gt;.

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  • 1P212 Morphological changes of the lipid membrane induced by inliposome membrane protein synthesis(13B. Biological & Artificial membrane: Dynamics,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Okamura Kosuke, Watanabe Hajime, Matsuura Tomoaki

    Seibutsu Butsuri   54 ( 1 )   S176   2014

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  • In vitro evolution of alpha-hemolysin using a liposome display Reviewed

    Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta, Tetsuya Yomo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   110 ( 42 )   16796 - 16801   2013.10

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    DOI： 10.1073/pnas.1314585110

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  • Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment Reviewed

    Norikazu Ichihashi, Kimihito Usui, Yasuaki Kazuta, Takeshi Sunami, Tomoaki Matsuura, Tetsuya Yomo

    NATURE COMMUNICATIONS   4   2494   2013.10

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    DOI： 10.1038/ncomms3494

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  • Kinetic model of double-stranded RNA formation during long RNA replication by Q beta replicase Reviewed

    Kimihito Usui, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo

    FEBS LETTERS   587 ( 16 )   2565 - 2571   2013.8

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  • 1P090 Directed Evolution of β-glucuronidase Using Liposome-based IVC(01F. Protein:Engineering,Poster)

    Nishikawa Takehiro, Sunami Takeshi, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   53 ( 1 )   S120   2013

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    DOI： 10.2142/biophys.53.S120_6

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  • 2P263 Directed evolution of a self-encoding system(20. Origin of life & Evolution,Poster)

    Sunami Takeshi, Ichihashi Norikazu, Nishikawa Takehiro, Kazuta Yasuaki, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   53 ( 1 )   S202   2013

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  • 1P091 Liposome Display : Directed evolution of membrane protein, alpha hemolysin, by using liposome and cell-free translation system(01F. Protein:Engineering,Poster)

    Fujii Satoshi, Matsuura Tomoaki, Sunami Takeshi, Kazuta Yasuaki, Yomo Tetsuya

    Seibutsu Butsuri   53 ( 1 )   S121   2013

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    DOI： 10.2142/biophys.53.S121_1

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  • alpha-Complementation in an Artificial Genome Replication System in Liposomes Reviewed

    Kotaro Nishiyama, Norikazu Ichihashi, Tomoaki Matsuura, Yasuaki Kazuta, Tetsuya Yomo

    CHEMBIOCHEM   13 ( 18 )   2701 - 2706   2012.12

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    DOI： 10.1002/cbic.201200586

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  • Fractal-shaped microchannel design for a kinetic analysis of biochemical reaction in a delay line Reviewed

    Katsuki Hirata, Tetsuo Ichii, Hiroaki Suzuki, Tomoaki Matsuura, Kazufumi Hosoda, Tetsuya Yomo

    MICROFLUIDICS AND NANOFLUIDICS   13 ( 2 )   273 - 278   2012.9

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    DOI： 10.1007/s10404-012-0958-y

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  • Effects of Compartment Size on the Kinetics of Intracompartmental Multimeric Protein Synthesis Reviewed

    Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Norikazu Ichihashi, Hiroaki Suzuki, Tetsuya Yomo

    ACS SYNTHETIC BIOLOGY   1 ( 9 )   431 - 437   2012.9

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    DOI： 10.1021/sb300041z

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  • Genomic Integration and Germline Transmission of Plasmid Injected into Crustacean Daphnia magna Eggs Reviewed

    Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe

    PLOS ONE   7 ( 9 )   e45318   2012.9

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    DOI： 10.1371/journal.pone.0045318

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  • Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules Reviewed

    Hiroaki Suzuki, Hidetoshi Terasawa, Kazuya Nishimura, Tomoaki Matsuura, Tetsuya Yomo

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   244   2012.8

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  • Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: Modeling of ligand-dependent and -independent expression Reviewed

    Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Tetsuya Yomo

    RNA-A PUBLICATION OF THE RNA SOCIETY   18 ( 8 )   1458 - 1465   2012.8

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    DOI： 10.1261/rna.032748.112

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  • Creating an artificial cell with different size revealed the effect of compartment volume on the intracompartmental multimeric protein synthesis Reviewed

    T. Matsuura, K. Hosoda, H. Suzuki, T. Yomo

    13th Int. Conf. Simulation & Synthesis of Living Systems (Afile 13)   619 - 620   2012.6

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  • Cell-Free Protein Synthesis inside Giant Unilamellar Vesicles Analyzed by Flow Cytometry Reviewed

    Koji Nishimura, Tomoaki Matsuura, Kazuya Nishimura, Takeshi Sunami, Hiroaki Suzuki, Tetsuya Yomo

    LANGMUIR   28 ( 22 )   8426 - 8432   2012.6

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    DOI： 10.1021/la3001703

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  • Construction of a Gene Screening System Using Giant Unilamellar Liposomes and a Fluorescence-Activated Cell Sorter Reviewed

    Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, Norikazu Ichihashi, Tetsuya Yomo

    ANALYTICAL CHEMISTRY   84 ( 11 )   5017 - 5024   2012.6

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    DOI： 10.1021/ac300678w

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  • Importance of Parasite RNA Species Repression for Prolonged Translation-Coupled RNA Self-Replication Reviewed

    Yohsuke Bansho, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    CHEMISTRY & BIOLOGY   19 ( 4 )   478 - 487   2012.4

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    DOI： 10.1016/j.chembiol.2012.01.019

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  • Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules Reviewed

    Hidetoshi Terasawa, Kazuya Nishimura, Hiroaki Suzuki, Tomoaki Matsuura, Tetsuya Yomo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   109 ( 16 )   5942 - 5947   2012.4

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    DOI： 10.1073/pnas.1120327109

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  • 2PT205 Directed evolution of a self-encoding system using giant liposome(The 50th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Ichihashi Norikazu, Nishikawa Takehiro, Kazuta Yasuaki, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   52   S139   2012

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    DOI： 10.2142/biophys.52.S139_5

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  • 1C1534 Construction of an in vitro gene screening system for membrane proteins(Proteins: Measurement, Analysis, Engineering,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

    Soga Haruka, Fujii Satoshi, Yomo Tetsuya, Watanabe Hajime, Matsuura Tomoaki

    Seibutsu Butsuri   52   S24   2012

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    DOI： 10.2142/biophys.52.S24_2

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  • Directed evolution of proteins through in vitro protein synthesis in liposomes Reviewed

    Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, Tetsuya Yomo

    Journal of Nucleic Acids   2012   923214   2012

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  • Cell-free protein synthesis from a single copy of DNA in a glass microchamber Reviewed

    Taiji Okano, Tomoaki Matsuura, Yasuaki Kazuta, Hiroaki Suzuki, Tetsuya Yomo

    LAB ON A CHIP   12 ( 15 )   2704 - 2711   2012

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    DOI： 10.1039/c2lc40098g

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  • Programmed Vesicle Fusion Triggers Gene Expression Reviewed

    Filippo Caschera, Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, Martin M. Hanczyc, Tetsuya Yomo

    LANGMUIR   27 ( 21 )   13082 - 13090   2011.11

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    DOI： 10.1021/la202648h

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  • Kinetic Analysis of beta-Galactosidase and beta-Glucuronidase Tetramerization Coupled with Protein Translation Reviewed

    Tomoaki Matsuura, Kazufumi Hosoda, Norikazu Ichihashi, Yasuaki Kazuta, Tetsuya Yomo

    JOURNAL OF BIOLOGICAL CHEMISTRY   286 ( 25 )   22028 - 22034   2011.6

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    DOI： 10.1074/jbc.M111.240168

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  • In Vitro Selection of Proteins that Undergo Covalent Labeling with Small Molecules by Thiol-Disulfide Exchange by Using Ribosome Display Reviewed

    Hayato Yanagida, Tomoaki Matsuura, Yasuaki Kazuta, Tetsuya Yomo

    CHEMBIOCHEM   12 ( 6 )   962 - 969   2011.4

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    DOI： 10.1002/cbic.201000620

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  • Quantitative screening system of beta-glucuronidase genes using unilamellar liposomes and cell sorter Reviewed

    Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, Norikazu Ichihashi, Tetsuya Yomo

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   241   2011.3

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  • Origin of lognormal-like distributions with a common width in a growth and division process Reviewed

    Kazufumi Hosoda, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    PHYSICAL REVIEW E   83 ( 3 )   031118   2011.3

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    DOI： 10.1103/PhysRevE.83.031118

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  • Evolvability and self-replication of genetic information in Liposomes Reviewed

    Tomoaki Matsuura, Norikazu Ichihashi, Takeshi Sunami, Hiroshi Kita, Hiroaki Suzuki, Tetsuya Yomo

    The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality   275 - 287   2011

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    DOI： 10.1007/978-90-481-9944-0_15

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  • 2F1624 Mere existence of growth/division yields cell-like size-homeostasis(Mathematical biology 2,The 48th Annual Meeting of the Biophysical Society of Japan)

    Hosoda Kazufumi, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   51   S84   2011

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    DOI： 10.2142/biophys.51.S84_4

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  • 3I1334 P29 On the effect of compartment size on the production of oligomeric proteins(3I Protein: Measurement & Analysis 2,The 49th Annual Meeting of the Biophysical Society of Japan)

    Matsuura Tomoaki, Hosoda Kazufumi, Ichihashi Norikazu, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   51   S138   2011

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    DOI： 10.2142/biophys.51.S138_2

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  • Giant unilamellar vesicles as a platform of liquid handing in femtoliter volumes

    Hidetoshi Terasawa, Hiroaki Suzuki, Kazuya Nishimura, Tomoaki Matsuura, Tetsuya Yomo

    15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011   3   1618 - 1620   2011

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  • Investigating Molecular Evolution in the Laboratory

    MATSUURA Tomoaki

    Seibutsu Butsuri   50 ( 6 )   270 - 271   2010.11

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    DOI： 10.2142/biophys.50.270

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  • Identification of Two Forms of Q beta Replicase with Different Thermal Stabilities but Identical RNA Replication Activity Reviewed

    Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Tetsuya Yomo

    JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 48 )   37210 - 37217   2010.11

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    DOI： 10.1074/jbc.M110.117846

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  • Cellular Compartment Model for Exploring the Effect of the Lipidic Membrane on the Kinetics of Encapsulated Biochemical Reactions Reviewed

    Takeshi Sunami, Kazufumi Hosoda, Hiroaki Suzuki, Tomoaki Matsuura, Tetsuya Yomo

    LANGMUIR   26 ( 11 )   8544 - 8551   2010.6

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    DOI： 10.1021/la904569m

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  • Constructing Partial Models of Cells Reviewed

    Norikazu Ichihashi, Tomoaki Matsuura, Hiroshi Kita, Takeshi Sunami, Hiroaki Suzuki, Tetsuya Yomo

    COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY   2 ( 6 )   a004945   2010.6

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    DOI： 10.1101/cshperspect.a004945

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  • Compartmentalization in a Water-in-Oil Emulsion Repressed the Spontaneous Amplification of RNA by Q beta Replicase Reviewed

    Hiroya Urabe, Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Hiroshi Kita, Tetsuya Yomo

    BIOCHEMISTRY   49 ( 9 )   1809 - 1813   2010.3

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    DOI： 10.1021/bi901805u

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  • 1P291 1H1325 Effects of cell size on internal self-replication of genetic information(Origin of life & Evolution,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)

    Bansho Yohsuke, Ichihashi Norikazu, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S71   2010

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    DOI： 10.2142/biophys.50.S71_2

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  • 2P250 Detection of association and fusion of giant vesicles using fluorescence-activated cell sorter(The 48th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Caschera Filippo, Morita Yuuki, Toyota Taro, Nishimura Kazuya, Matsuura Tomoaki, Suzuki Hiroaki, Hanczyc Martin M., Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S126 - S127   2010

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    DOI： 10.2142/biophys.50.S126_6

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  • 1P070 Co-translational folding of beta-galactosidase and beta-glucuronidase in an in vitro translation system(Protein:Property,The 48th Annual Meeting of the Biophysical Society of Japan)

    Matsuura Tomoaki, Hosoda Kazufumi, Ichihashi Norikazu, Kazuta Yasuaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S31   2010

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    DOI： 10.2142/biophys.50.S31_4

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  • 2P346 A basic understanding for cell size homeostasis(The 48th Annual Meeting of the Biophysical Society of Japan)

    Hosoda Kazufumi, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S143 - S144   2010

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    DOI： 10.2142/biophys.50.S143_5

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  • 2P102 In vitro selection for covalent binding via disulfide interchange with ribosome display(The 48th Annual Meeting of the Biophysical Society of Japan)

    yanagida hayato, matsuura tomoaki, kazuta yasuaki, yomo tetsuya

    Seibutsu Butsuri   50 ( 2 )   S100   2010

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    DOI： 10.2142/biophys.50.S100_2

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  • Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection. Reviewed

    Yanagida H, Matsuura T, Yomo T

    Methods in molecular biology (Clifton, N.J.)   634   257 - 267   2010

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  • Synthesis of functional proteins within liposomes. Reviewed

    Sunami T, Matsuura T, Suzuki H, Yomo T

    Methods in molecular biology (Clifton, N.J.)   607   243 - 256   2010

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  • 2P241 Budding transition of giant unilamellar vesicles after electro-fusion(The 48th Annual Meeting of the Biophysical Society of Japan)

    Terasawa Hidetoshi, Nishimura Kazuya, Suzuki Hiroaki, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S125   2010

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    DOI： 10.2142/biophys.50.S125_2

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  • Population Analysis of Structural Properties of Giant Liposomes by Flow Cytometry Reviewed

    Kazuya Nishimura, Tomohiro Hosoi, Takeshi Sunami, Taro Toyota, Masanori Fujinami, Koichi Oguma, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    LANGMUIR   25 ( 18 )   10439 - 10443   2009.9

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    DOI： 10.1021/la902237y

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  • Requirements for Efficient Replication of Genetic Information in a Translation-Coupled Replication System Reviewed

    Norikazu Ichihashi, Hiroshi Kita, Kazufumi Hosoda, Takeshi Sunami, Koji Tsukada, Tomoaki Matsuura, Tetsuya Yomo

    ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES   39 ( 3-4 )   317 - 318   2009.8

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  • Quantifying epistatic interactions among the components constituting the protein translation system Reviewed

    Tomoaki Matsuura, Yasuaki Kazuta, Takuyo Aita, Jiro Adachi, Tetsuya Yomo

    MOLECULAR SYSTEMS BIOLOGY   5   297   2009.8

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    DOI： 10.1038/msb.2009.50

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  • 2TA3-05 Exploring the fitness landscape of the protein translation system(The 47th Annual Meeting of the Biophysical Society of Japan)

    Matsuura Tomoaki, Kazuta Yasuaki, Aita Takuyo, Adachi Jiro, Yomo Tetsuya

    Seibutsu Butsuri   49   S42   2009

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    DOI： 10.2142/biophys.49.S42_2

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  • 1YP1-01 What is the condition of realizing a self-replication system of genetic information in vitro?(1YP1 Early Research in Biophysics Award Candidate Presentations,The 47th Annual Meeting of the Biophysical Society of Japan)

    Ichihashi Norikazu, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S1   2009

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    DOI： 10.2142/biophys.49.S1_1

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  • 1P-254 Fractal-shaped micro channel system for kinetic analysis of biochemical reaction(Measurements, The 47th Annual Meeting of the Biophysical Society of Japan)

    Hirata Katsuki, Ichii Tetsuo, Suzuki Hiroaki, MaTsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S102   2009

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    DOI： 10.2142/biophys.49.S102_2

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  • 2P-228 What is the condition of realizing a self-replication system of genetic information in vitro?(Origin of life & Evolution,The 47th Annual Meeting of the Biophysical Society of Japan)

    Ichihashi Norikazu, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S143   2009

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    DOI： 10.2142/biophys.49.S143_2

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  • 1P-189 Liposome fusion induced by charged lipids incorporated in the preformed liposomes using the ethanol injection method(Biol & Artifi memb.:Dynamics, The 47th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Caschera Filippo, Morita Yuki, Nishimura Kazuya, Toyota Taro, Hanczyc Martin, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S92   2009

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    DOI： 10.2142/biophys.49.S92_2

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  • 1P-184 Quantitative evaluation of biochemical reactions encapsulated in giant unilamellar liposomes(Biol & Artifi memb.:Structure & Property, The 47th Annual Meeting of the Biophysical Society of Japan)

    Nishimura Koji, Sunami Takeshi, Suzuki Hiroaki, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S91   2009

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    DOI： 10.2142/biophys.49.S91_2

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  • DETECTION AND ANALYSIS OF PROTEIN SYNTHESIS AND RNA REPLICATION IN GIANT LIPOSOMES Reviewed

    Takeshi Sunami, Hiroshi Kita, Kazufumi Hosoda, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    METHODS IN ENZYMOLOGY; LIPOSOMES, PT F   464   19 - 30   2009

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    DOI： 10.1016/S0076-6879(09)64002-7

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  • Quantitative Study of the Structure of Multilamellar Giant Liposomes As a Container of Protein Synthesis Reaction Reviewed

    Kazufumi Hosoda, Takeshi Sunami, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    LANGMUIR   24 ( 23 )   13540 - 13548   2008.12

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    DOI： 10.1021/la802432f

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  • 3P-278 Requirements for the efficient self-replication system of genetic information(The 46th Annual Meeting of the Biophysical Society of Japan)

    Ichihashi Norikazu, Matsuura Tomoaki, Kazuta Yasuaki, Kita Hiroshi, Yomo Tetsuya

    Seibutsu Butsuri   48   S170   2008

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    DOI： 10.2142/biophys.48.S170_5

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  • 3P-280 Quantifying contributions of inter-component interactions on the activity of protein translation system(The 46th Annual Meeting of the Biophysical Society of Japan)

    Kawamura Atsushi, Matsuura Tomoaki, Kazuta Yasuaki, Aita Takuyo, Adachi Jiro, Yomo Tetsuya

    Seibutsu Butsuri   48   S170 - S171   2008

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    DOI： 10.2142/biophys.48.S170_7

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  • 3P-281 Prediction of the protein translation activity values by estimating Bahadur coefficients from a small number of experimental data set(The 46th Annual Meeting of the Biophysical Society of Japan)

    Kazuta Yasuaki, Matsuura Tomoaki, Aita Takuyo, Adachi Jiro, Yomo Tetsuya

    Seibutsu Butsuri   48   S171   2008

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    DOI： 10.2142/biophys.48.S171_1

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  • 3P-275 Quantitative analysis of interactions between the phospholipid membrane and encapsulated reaction systems in cell-sized liposomes(The 46th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Hosoda Kazufumi, Kita Hiroshi, Ichihashi Norikazu, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   48   S170   2008

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    DOI： 10.2142/biophys.48.S170_2

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  • Kinetic analysis of the entire RNA amplification process by Q beta replicase Reviewed

    Kazufumi Hosoda, Tomoaki Matsuura, Hiroshi Kita, Norikazu Ichihashi, Koji Tsukada, Tetsuya Yomo

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 21 )   15516 - 15527   2007.5

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    DOI： 10.1074/jbc.M700307200

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  • 3P271 RNA-protein self-replicating system in liposome(The genesis of life, and biological evolution,Poster Presentations)

    Kita Hiroshi, Sunami Takeshi, Matsuura Tomoaki, Hosoda Kazufumi, Ichihashi Norikazu, Tsukada Koji, Urabe Itaru, Yomo Tetsuya

    Seibutsu Butsuri   47   S270   2007

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    DOI： 10.2142/biophys.47.S270_4

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  • 3P274 Experimental evolution of a primordial DNA binding protein(Proteins- protein engineering, and evolutionary engineering,Poster Presentations)

    Ishikawa Keitaro, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   47   S271   2007

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    DOI： 10.2142/biophys.47.S271_3

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  • 1P236 Network topology of protein translation system and its evolution(Bioinformatics-functional genomics,Poster Presentations)

    Adachi Jiro, Ono Naoki, Kazuta Yasuaki, Matsuura Tomoaki, Urabe Itaru, Mori Hirotada, Yomo Tetsuya

    Seibutsu Butsuri   47   S82   2007

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    DOI： 10.2142/biophys.47.S82_3

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  • 1P235 Comprehensive analysis on the effect of Escherichia coli ORFs on protein translation reaction(Bioinformatics-functional genomics,Poster Presentations)

    Kazuta Yasuaki, Adachi Jiro, Matsuura Tomoaki, Urabe Itaru, Mori Hirotada, Yomo Tetsuya

    Seibutsu Butsuri   47   S82   2007

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    DOI： 10.2142/biophys.47.S82_2

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  • 2P270 Inner aqueous volume distributions of cell-size liposomes in consideration of the inner-compartmentalization(Native and artificial biomembranes-structure and properties,Poster Presentations)

    Hosoda Kazufumi, Sunami Takeshi, Matsuura Tomoaki, Kita Hiroshi, Ichihashi Norikazu, Tsukada Koji, Yomo Tetsuya

    Seibutsu Butsuri   47   S180   2007

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    DOI： 10.2142/biophys.47.S180_3

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  • Functional Q beta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit Reviewed

    Hiroshi Kita, Junghwa Cho, Tomoaki Matsuura, Tomoyuki Nakaishi, Ichiro Taniguchi, Takehiko Ichikawa, Yasuhumi Shima, Itaru Urabe, Tetsuya Yomo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   101 ( 5 )   421 - 426   2006.5

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    DOI： 10.1263/jbb.101.421

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  • 2P134 Nascent chain, RNA, and ribosome complexes generated by reconstituted in vitro translation system(32. Evolutionary molecular engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Yanagida Hayato, Matsuura Tomoaki, Ushioda Junya, Urabe Itaru, Yomo Tetsuya

    Seibutsu Butsuri   46 ( 2 )   S329   2006

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    DOI： 10.2142/biophys.46.S329_2

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  • 2P438 Strategy to evaluate the effect of individual E. coli protein on the protein translation machinery(48. Bioinformatics, genomics and proteomics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Adachi Jiro, Kazuta Yasuaki, Matsuura Tomoaki, Urabe Itaru, Yomo Tetsuya

    Seibutsu Butsuri   46 ( 2 )   S405   2006

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    DOI： 10.2142/biophys.46.S405_2

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  • 1P119 kinetic study of reconstituted translation-replication system

    Kita H., Hosoda K., Matsuura T., Tsukada K., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S61   2005

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    DOI： 10.2142/biophys.45.S61_3

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  • 3PO77 In vitro compartmentalization by liposome : sorting and gene selection by FACS

    Sunami T., Sato K., Tsukada K., Matsuura T., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S223   2005

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.45.S223_1

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  • 2P141 Kinetics of +1 Ribosomal Frameshifthing

    Matsuura T., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S155   2005

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    DOI： 10.2142/biophys.45.S155_1

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  • 1P126 Enzymology of Q beta replicase: Template RNA catches "Q beta replicase" in a trap

    Hosoda K., Kita H., Matsuura T., Tsukada K., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S63   2005

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.45.S63_2

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  • 3P080 Directed evolution of active RNA dependent RNA polymerase with the aid of green fluorescent protein folding reporter

    Takita M., Ito Y., Tsukada K., Matsuura T., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S223   2005

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.45.S223_4

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  • 3P079 Directed evolution of unstructured protein based on ligand binding property

    Yanagida H., Matsuura T., Tamura A., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S223   2005

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    DOI： 10.2142/biophys.45.S223_3

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  • 3P078 Function of DNA binding protein Zif268 is tolerant to domain substitution

    Nobuhara K., Matsuura T., Urabe I., Yomo T.

    Seibutsu Butsuri   45   S223   2005

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    DOI： 10.2142/biophys.45.S223_2

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  • Evolutionary molecular engineering by random elongation mutagenesis. Reviewed

    Matsuura T, Yomo T, Urabe I

    Methods in molecular biology (Clifton, N.J.)   182   221 - 230   2002

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  • Evolutionary molecular engineering by random elongation mutagenesis Reviewed

    T Matsuura, K Miyai, S Trakulnaleamsai, T Yomo, Y Shima, S Miki, K Yamamoto, Urabe, I

    NATURE BIOTECHNOLOGY   17 ( 1 )   58 - 61   1999.1

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  • Fragmentation of DNA molecules depends on their concentration.

    Tadagaki K., Hirano F., Matsuura T., Yomo T., Urabe I.

    Seibutsu Butsuri   39   S182   1999

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.39.S182_2

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  • Effect of DNA molecule numbers on sustainability of self-replicating system.

    Shibuya N., Yamaguchi M., Matsuura T., Yomo T., Urabe I.

    Seibutsu Butsuri   39   S201   1999

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.39.S201_1

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  • Nonadditivity of mutational effects on the properties of catalase I and its application to efficient directed evolution Reviewed

    T Matsuura, T Yomo, S Trakulnaleamsai, Y Ohashi, K Yamamoto, Urabe, I

    PROTEIN ENGINEERING   11 ( 9 )   789 - 795   1998.9

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MISC

  • 1P-029 Efficient DNA integration into germ-line genome using TALEN in Daphnia magna

    Nakanishi Takashi, Kato Yasuhiko, Matsuura Tomoaki, Watanabe Hajime

    67   96 - 96   2015

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  • 2S-Ba03 In vitro protein evolution using the biointerface

    Matsuura Tomoaki

    67   247 - 247   2015

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  • 1C33 Volume Dependence of Cell-free Protein Synthesis Using a Glass Microchamber

    OKANO Taiji, MATSUURA Tomoaki, SUZUKI Hiroaki, YOMO Tetsuya

    2014 ( 26 )   91 - 92   2014.1

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    Language：Japanese   Publisher：The Japan Society of Mechanical Engineers  

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  • 1P-041 Targeted mutagenesis using CRISPR/Cas and TALEN in Daphnia magna

    Nakanishi Takashi, Naitou Akiko, Kato Yasuhiko, Matsuura Tomoaki, Watanabe Hajime

    66   27 - 27   2014

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  • J026031 Measurement of the protein synthesis from a single copy of DNA in the microwell

    OKANO Taiji, MATSUlfRA Tomoaki, KAZUTA Yasuaki, SUZUKI Hiroaki, YOMO Tetsuya

    Mechanical Engineering Congress, Japan   2013   "J026031 - 1"-"J026031-4"   2013.9

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    Language：Japanese   Publisher：The Japan Society of Mechanical Engineers  

    We fabricated quartz microchambers for performing protein synthesis using a reconstituted in vitro transcription-translation system. By using glass microchambers, the yield of the green fluorescent protein (GFP) synthesis was significantly improved compared to that obtained in the widely-used poly(dimethylsiloxane) (PDMS)microchambers. Using this device, we demonstrated that GFP can be synthesized from a single copy of DNA. Quantized and distinctive signals from proteins synthesized from 0, 1, or 2 copies of genes were obtained. The microchamber presented here can be used for not only studying the effects of compartment volume on protein synthesis but also the comprehensive analysis of complex biochemical reactions in cell-mimetic environments.

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  • 4Cp11 Construction of an in vitro gene screening system for membrane proteins

    Soga Haruka, Fujii Satoshi, Yomo Tetsuya, Watanabe Hajime, Matsuura Tomoaki

    日本生物工学会大会講演要旨集   64   197 - 197   2012.9

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    Language：Japanese   Publishing type：Research paper, summary (national, other academic conference)   Publisher：The Society for Biotechnology, Japan  

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  • Protein Synthesis from a Single-molecule of DNA in a Glass Microchamber

    OKANO Taiji, MATSUURA Tomoaki, KAZUTA Yasuaki, SUZUKI Hiroaki, YOMO Tetsuya

    2012 ( 8 )   1 - 6   2012.6

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  • 4Gp08 Establishment of a Quantification Method by Live Imaging of Daphnia magna

    Suzuki Akiko, Matsuura Tomoaki, Watanabe Hajime

    64   225 - 225   2012

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  • 2Dp18 Kinetic analysis of β-galactosidase and β-glucuronidase tetramerization coupled with protein translation

    Matsuura Tomoaki, Hosoda kazufumi, Ichihashi Norikazu, Kazuta Yasuaki, Yomo Tetsuya

    63   139 - 139   2011

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  • MEASUREMENT OF NONLINEAR BIOCHEMICAL REACTION IN MICRODROPLETS USING THE FRACTAL-SHAPED MICRO CHANNEL.

    2010

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  • 進化能を持つ遺伝子複製系をつくる

    2010

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  • The Minimal Cell : The Biophysics of Cell Compartment and the Origin of Cell Functionality

    Springer   2010

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  • Effects of cell size on internal self-replication of genetic information.

    2010

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  • Constructing an artificial self-replication system of genetic information from RNA and proteins.

    2010

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  • In Vitro Mutagenesis Protocols Third Edition

    Humana Press   2010

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  • The Origins of Life

    COLD SPRING HARBOR LABORATORY PRESS   2010

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  • The Minimal Cell : The Biophysics of Cell Compartment and the Origin of Cell Functionality

    Springer   2010

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  • MEASUREMENT OF NONLINEAR BIOCHEMICAL REACTION IN MICRODROPLETS USING THE FRACTAL-SHAPED MICRO CHANNEL.

    2010

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  • Comparing Epistasis of a Protein and Protein Synthesis System

    2010

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  • Constructing an artificial self-replication system of genetic information from RNA and proteins.

    2010

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  • Effects of cell size on internal self-replication of genetic information.

    2010

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  • In Vitro Mutagenesis Protocols Third Edition

    Humana Press   2010

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  • Comparing Epistasis of a Protein and Protein Synthesis System

    2010

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  • 遺伝子の自己複製反応に与える反応場サイズ依存症

    2010

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  • 単層膜ジャイアントリポソーム内タンパク質合成反応の評価

    2010

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  • 進化する遺伝情報の自己複製システムの構築

    2010

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  • 2Gp28 Elucidating the strategy for optimizing the cell-free protein synthesis system

    MATSUURA TOMOAKI, KAZUTA YASUAKI, ADACHI JIRO, AITA TAKUYO, ONO NAOAKI, MORI HIROTADA, YOMO TETSUYA

    21   99 - 99   2009

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  • 蛍光セルソータを用いたリポソーム構造および内部反応のダイナミクス計測

    2008

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  • 遺伝情報が自己複製するために必要な条件を明らかにする

    2008

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  • RNAとタンパク質からなる人工自己複製系の速度論的解析

    2007

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  • In vitro evolution of proteins

    Tomoaki Matsuura, Tetsuya Yomo

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   101 ( 6 )   449 - 456   2006.6

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    Language：English   Publishing type：Book review, literature introduction, etc.  

    DOI： 10.1263/jbb.101.449

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  • Nascent chain, RNA, and ribosome complexes generated by reconstituted in vitro translation system

    2006

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  • Strategy to evaluate the effect of individual E.coli protein on the protein translation machinery

    2006

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  • Real-time detection of translation frameshifting using PURE system

    2006

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  • A new mechanism of the RNA synthesis reaction by Q beta replicase: Q beta replicase irreversibly inactivated by the interaction with the template RNA

    525   2006

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  • Combinatorial approaches to novel proteins

    T Matsuura, A Ernst, DL Zechel, A Pluckthun

    CHEMBIOCHEM   5 ( 2 )   177 - 182   2004.2

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  • 2A12-3 A combinatorial and evolutionary approach to novel proteins

    MATSUURA Tomoaki, TOKURIKI Nobuhiko, YOMO Tetsuya, URABE Itaru, PLUCKTHUN Andreas

    16   107 - 107   2004

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  • ニューバイオフィジックスⅡ－８ 生命の起源と進化の物理学

    共立出版   2003

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  • Construction and characterization of protein libraries composed of secondary structure modules

    T Matsuura, A Ernst, A Pluckthun

    PROTEIN SCIENCE   11 ( 11 )   2631 - 2643   2002.11

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  • Evolutionary molecular engineering by random elongation mutagenesis. In: Methods in Molecular Biology, 2nd edn., vol.182, Braman, J. (ed.)

    Humana Press Inc.   2001

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  • Nonaddirivity of Mutational Effects on the properties of Catalase Iand lts Application to Efficient Directed Evolution

    Matsuura Tomoaki, Trakulnaleamsai Savitr, Yomo Tetsuya, Urabe Itaru

    Biophysics   38 ( 2 )   S20   1998.9

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

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  • Artificial random protein : the solubility and secretion by heat

    MATSUURA T., KAWAMA T., YOSHIUCHI K., YOMO T., URABE I.

    Biophysics   37   S29   1997.10

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  • Effect of the elongated amino acid sequence on the property of the enzyme

    Matsuura T., Trakulnaleamsai Savitr, Miyai K., Yomo T., Urabe I.

    9   139 - 139   1997

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  • 無細胞自己複製系におけるダイナミクス(ポスター発表,基研長期研究会「複雑系」,研究会報告)

    松浦 友亮, 四方 哲也

    物性研究   63 ( 6 )   770 - 770   1995

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    Language：Japanese   Publisher：物性研究刊行会  

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    Other Link： http://hdl.handle.net/2433/95504

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Awards

  • 第52回 日本生物工学会研究奨励賞（斎藤賞）

    2016.9   日本生物工学会   「セルフリータンパク質合成系を用いた進化分子工学技術の開発」

    松浦 友亮

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  • 第4回 大阪大学総長奨励賞（研究部門）

    2015.7  

    松浦 友亮

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  • 第3回 大阪大学総長奨励賞（研究部門）

    2014.7  

    松浦 友亮

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  • 酵素工学研究会奨励賞

    2009.4   日本酵素工学研究会   「無細胞翻訳系を利用したタンパク質の機能改変に関する研究」

    松浦 友亮

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Research Projects

  • Understanding of the diversity of prebiotic chemistries and emergence of molecular systems in various planetary environments in the Solar System

    Grant number：22K21344  2022.12 - 2029.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (International Leading Research )

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    Grant amount：\689000000 （ Direct Cost: \530000000 、 Indirect Cost：\159000000 ）

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  • Bottom-up creation of cell-free molecular systems: surpassing nature

    Grant number：21H05228  2021.9 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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    Grant amount：\226200000 （ Direct Cost: \174000000 、 Indirect Cost：\52200000 ）

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  • Bottom-up creation of cell-free molecular systems: surpassing nature

    Grant number：21A205  2021.9 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)

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  • 超越分子システム領域の総括班

    Grant number：21H05225  2021.9 - 2026.3

    日本学術振興会  科学研究費助成事業  学術変革領域研究(A)

    松浦 友亮

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    Grant amount：\25220000 （ Direct Cost: \19400000 、 Indirect Cost：\5820000 ）

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  • Design of beta-barrel type transmembrane protein complex by combination of computational and experimental studies

    Grant number：19KK0178  2019.10 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

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    Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）

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  • Novel membrane proteins by combining computational design and in vitro directed eovlution

    Grant number：17H00888  2017.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Matsuura Tomoaki

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    Grant amount：\43160000 （ Direct Cost: \33200000 、 Indirect Cost：\9960000 ）

    Only a small fraction of the sequence space has been explored by nature through evolution. By rational design, it is now clear that for soluble proteins, there are many artificial proteins that possess structures and functions in the unexplored sequence space. In this study, we show that there are molecules with artificial membrane proteins that also have functions and structures in membrane proteins. Specifically, we designed an artificial membrane protein with a 2 nm pore and clarified its function using an artificial cell reactor.

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  • リポソーム内膜タンパク質発現系の発現ダイナミクス制御技術の確立と応用

    Grant number：16H00767  2016.4 - 2018.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    松浦 友亮

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    Grant amount：\6370000 （ Direct Cost: \4900000 、 Indirect Cost：\1470000 ）

    我々は、細胞サイズのリポソーム内で再構成型無細胞翻訳系PURE systemを用いて膜タンパク質を合成する技術を確立してきた。リポソームディスプレイ法と名付けたこの技術は、膜タンパク質の進化分子工学だけでなく、細胞環境に近い反応場での膜タンパク質の機能解析を可能とする。
    生体膜上での高次の生命現象の多くは、発現のダイナミクスが制御された複数種の膜タンパク質が協同的に働くことで進行する。現行のリポソームディスプレイ法は、複数種の膜タンパク質を発現させることは可能だが、各タンパク質の発現のタイミングを制御できない。そこで本研究では、光によって異性化するアゾベンゼン2,6-dimethylazobenzene-4’-carboxylic acid (DM-Azo)と2,6-dimethyl-4-(methylthio)azobenzene-4′-carobxylic acid (S-DM-Azo)を導入したオリゴDNAを用いる事でリポソーム内膜タンパク質合成反応のダイナミクスを制御する技術の確立とその応用を目指した。
    まず、緑色蛍光タンパク質（GFP）をモデルタンパク質として用い、その発現の光制御技術の確立を行った。オリゴDNA配列をGFP遺伝子の開始コドン付近に会合し、リボソームによる翻訳反応を阻害するようデザインした。その結果、DM-Azoを用いたオリゴDNAのほうがS-DM-Azoよりも合成反応を効率的に停止させる事、オリゴへの適切なDM-Azo挿入位置を明らかにした。次に、上記の情報を用いて膜タンパク質の膜透過装置Secトランスロコンとモデル膜タンパク質である大腸菌由来多剤排出トランスポーターEmrEの発現の制御を試みた。SecトランスロコンとEmrEを両方同時に合成し、光照射によりSecトランスロコンの翻訳のみを停止させる事に成功した。リポソーム内で、この反応を実施する事により、Secトランスロコンの存在下に於いてEmrEを合成すること、すなわち細胞模擬環境が実現可能となる。

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  • Constructing an in vitro screening system of peptides that inhibit the function of membrane proteins

    Grant number：15K12756  2015.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Matsuura Tomoaki

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    We aim to construct an in vitro screening system for peptides that inhibit the function of membrane protein of interest. The method is based on the syntheses of membrane protein of interest and the inhibitor peptide simultaneously using an in vitro transcription-translation system inside cell-sized phospholipid vesicles. Here, we first created a high quality random peptide library with a length of 10 amino acids. Then using EmrE, a multidrug transporter from Escherichia coli as a model protein, the screening system of an inhibitor peptide was established. By combining the constructed peptide library and the gene screening system, we now can screen inhibitor peptides entirely in vitro.

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  • Grow hair from liposomes

    Grant number：26630426  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    HORI KATSUTOSHI

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    Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）

    The purpose of this study is to synthesize the adhesive nanofiber protein AtaA on Acinetobacter sp. Tol 5 cells inside the liposome and grow it on the surface. We constructed a gene of shortened ataA which folds correctly without compromising the basic structure of AtaA and confirmed the synthesis of polypeptide chain in a cell-free protein synthesis system. Next, the reduced ataA gene was expressed inside the cell-sized giant single lamellar liposome, and synthesis of the shortened AtaA protein and presentation of liposome surface layer were attempted. Flow cytometry showed the possibility that a trace amount of the shortened AtaA fluorescently labeled by the antibody was displayed on the liposome surface, but it was not a level that could be said to be definitely presented.

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  • リポソーム内膜タンパク質合成系を用いた細胞膜動態の再構成

    Grant number：26102528  2014.4 - 2016.3

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)  新学術領域研究(研究領域提案型)

    松浦 友亮

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    Grant amount：\5980000 （ Direct Cost: \4600000 、 Indirect Cost：\1380000 ）

    本研究では、無細胞翻訳系を用いて細胞サイズ（1-5 マイクロメートル）のリポソーム内外でヒトのミトコンドリア由来膜タンパク質Letm1を合成し、Letm1の機能発現のダイナミクスを調べることを目的とした。
    これまでにリポソーム内部でLetm1を合成した際、C末端に蛍光タンパク質mCherryを融合させた遺伝子を用いることで、Letm1の膜への局在を確認した。加えて、リポソームサイズの変化がリポソームの脂質組成に依存することもわかった。
    Letm1はプロトンと共役してCa2+イオンを輸送するカルシウムチャネルである。そこで、Ca2+イオンの輸送活性を検出する実験系を構築し、リポソームの脂質組成とCa2+輸送活性の関係性を明らかにした。Ca2+イオンを検出するためには、複数の蛍光インジケータを検討し、Fluo4を用いた場合に最も強いシグナルが観測されたためこれを選択した。次に、ミトコンドリア内膜を構成する主要な4種類の脂質、ホスファチジルコリン(PC)、ホスファチジルエタノールアミン(PE)、カルジオリピン(CL)、ホスファチジルイノトール(PI)を混ぜて作製したリポソーム内でLetm1を合成した。その結果、Letm1のリポソーム膜へ局在及びCa2+の輸送活性が確認された。このように活性型のLetm1を合成可能な実験条件を明らかにした。次に、Letm1が機能を発現するメカニズムの詳細を明らかにするために、Ca2+の輸送活性の脂質依存性を調べた。その結果、PC, PE, CL, PIの全てが存在するときにのみ活性が見られた。この結果は、Letm1の機能発現するためには脂質分子が重要な役割を果たしており、またこれらの脂質が協同的に働いていることを示している。

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  • Directed evolution of membrane proteins using cell-free protein synthesis system

    Grant number：25282239  2013.4 - 2016.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Matsuura Tomoaki

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    Grant amount：\18850000 （ Direct Cost: \14500000 、 Indirect Cost：\4350000 ）

    Liposome display is an evolutionary method that enables the directed evolution of membrane proteins in vitro. The method is based on the syntheses of membrane proteins using an in vitro transcription&#8211;translation system (IVTT) inside cell-sized phospholipid vesicles. Here, using EmrE, a multidrug transporter from Escherichia coli, as a model protein, the screening was performed based on two functions of EmrE: substrate transport activity and membrane integration activity. Starting from a mock gene library prepared by mixing an active and an inactive gene, 10- to 35-fold enrichment of the active genes was obtained. In addition, from a random mutagenized library of EmrE, gene pool exhibiting higher activity than the wild-type was obtained. We also succeeded in introducing the membrane protein transport machinery to the liposome display method, which expanded the repertories of the membrane protein that can be applied to liposome display.

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  • The effect of compartment size on the intracompartmental reaction.

    Grant number：23700361  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MATSUURA Tomoaki

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

    The cell contents are encapsulated within a compartment, the volume of which is a fundamental physical parameter that may affect intracompartmental reactions. However, there have been few studies to elucidate whether and how volume changes alone ca n affect the reaction kinetics. Here, we prepared artificial cell-like compartments with different volumes, and encapsulated the in vitro protein synthesis system. The effect of the compartment size on the protein synthesis reaction was investigated. We found a reaction that proceeds faster in smaller compartments and revealed the mechanism of the acceleration. This result provided an example of how small compartment can be beneficial for the intracompartmental reactions.

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  • リポソーム内における生化学反応の検出及びその解析

    Grant number：19021029  2007

    日本学術振興会  科学研究費助成事業 特定領域研究  特定領域研究

    松浦 友亮

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    Grant amount：\900000 （ Direct Cost: \900000 ）

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  • （１）分子進化工学的手法の開発・改良 （２）蛋白質の人工進化 （３）蛋白質翻訳機構の解明

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    Grant type：Competitive

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