Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsami.5c10623

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1038/s41467-025-64946-2

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Other Link： https://www.nature.com/articles/s41467-025-64946-2

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.snb.2025.138098

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Despite rapid advances in soft robotics for biomedical applications, little attention has been paid to its potential for simulating interactive nursing procedures that involve complex human responses. In this study, the first soft robotic simulator was specifically designed for training in the digital removal of feces (DRF)—a delicate, discomfort‐sensitive nursing technique frequently performed in home care settings in Japan. Existing DRF training relies on static models that fail to replicate the physiological responses of patients, often leading to inadequate skill acquisition and patient discomfort. To address this gap, we implement a novel simulator incorporating two soft actuators (a pouch‐type actuator for anal sphincter contraction and a McKibben muscle for puborectalis relaxation) and two soft sensors for detecting anal and rectal massage. These are embedded into a multilayer rectal model and integrated with a real‐time feedback interface. Our design uniquely prioritizes both functional fidelity and perceptual realism, enabling trainees to learn from physiological reactions that mimic actual patient responses. Validation with experienced and novice nurses confirms the simulator's effectiveness in improving procedural understanding and confidence. This study demonstrates a new application of soft robotics—not only for anatomical replication, but also as a practical training tool for hands‐on nursing procedures.

DOI： 10.1002/adrr.202400026

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Abstract

Soft robotic devices use heat energy widely as both a power source and a functional interface. In addition, soft robots are expected to be applicable in medical and healthcare settings because of their flexibility and biocompatibility. If a small, flexible device that can generate thermal energy is developed, this device is expected to be applied as a wearable, implantable soft robotic device. This study introduces a thin‐film‐type heat‐generating device that uses laser‐induced graphene (LIG) on a poly(D, L‐lactic) acid film and achieves both resistance heating by application of a direct current and photothermal conversion heating by irradiation with near‐infrared light. This LIG and poly(D, L‐lactic) acid composite device is composed of a thin film structure with a thickness of ≈120 µm, and in addition to a simple heating method based on the application of a direct current‐type voltage, the device can also be heated by irradiating it with near‐infrared light, regardless of the LIG design. In addition, because this device offers a high degree of design freedom, it is expected to be used as a flexible and biocompatible composite heating element.

DOI： 10.1002/aelm.202500062

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsbiomaterials.4c02468

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsabm.4c01446

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

We develop a graphene-based resonant sensor to enhance the resonant properties by applied strain. An aptamer-modified sensor exhibited a frequency shift in response to inactivated SARS-CoV-2 virus, demonstrating a mass sensitivity of 0.99 zg Hz⁻¹.

DOI： 10.1039/d4nr04571h

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

Adhesive coating based on gallol and a urea precursor enhanced adhesiveness due to abundant hydrogen bonds without impairing the stretchability of elastomers.

DOI： 10.1039/d4ma01102c

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Mary Ann Liebert Inc  

DOI： 10.1089/wound.2024.0014

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Other Link： https://www.liebertpub.com/doi/pdf/10.1089/wound.2024.0014

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Abstract

Polysaccharide‐based hydrogels are promising for biomedical applications such as drug delivery owing to their biocompatibility, biodegradability, and bioactivities. In particular, there is a need for hydrogels with injectability for minimally invasive therapies and controlled sustained release for sustained drug effect. Hydrogels made from carboxymethyl chitosan (CMC) and tannic acid (TA) (CMC‐TA) contain dynamic covalent bonds owing to autoxidation between CMC and TA, and interact with proteins via TA, elucidation of the detailed mechanism of dynamic covalent bonding in CMC‐TA hydrogels will facilitate stable loading and zero‐order release of proteins. Herein, the physical properties of oxidized CMC‐TA (Oxi‐CMC‐TA) prepared for the encapsulation and sustained release of proteins are explored. Following incubation at 37 °C for 24 h, CMC‐TA undergoes secondary crosslinking by autoxidation to produce Oxi‐CMC‐TA. Notably, CMC‐TA is viscoelastic and shear‐thinning, allowing for injection and 3D bioprinting. Indeed, CMC‐TA can be 3D‐printed with green fluorescent protein (GFP) encapsulated in its matrix. Oxi‐CMC‐TA also exhibits an affinity for protein owing to its gallol groups, enabling Oxi‐CMC‐TA to collect GFP in aqueous solutions against a concentration gradient. Moreover, Oxi‐CMC‐TA releases GFP over 15 days. Injectable, 3D‐printable, protein‐collecting, and zero‐order sustained‐releasing Oxi‐CMC‐TA has the potential to make a significant contribution to drug delivery.

DOI： 10.1002/macp.202400220

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Language：English   Publishing type：Research paper (scientific journal)  

Cell encapsulation devices are expected to be promising tools that can control the release of therapeutic proteins secreted from transplanted cells. The protein permeability of the device membrane is important because it allows the isolation of transplanted cells while enabling the effectiveness of the device. In this study, we investigated free-standing polymeric ultra-thin films (nanosheets) as an intrinsically semi-permeable membrane made from polydimethylsiloxane (PDMS). The PDMS nanosheet with a thickness of 600 nm showed intrinsic protein permeability, and the device fabricated with the PDMS nanosheet showed that VEGF secreted from implanted adipose tissue-derived stem cells (ASCs) could be released for at least 5 days. The ASC encapsulation device promoted angiogenesis and the development of granulation tissue 1 week after transplantation to the subcutaneous area of a mouse. This cell encapsulation device consisting of PDMS nanosheets provides a new method for pre-vascularization of the subcutaneous area in cell transplantation therapy.

DOI： 10.1039/d4bm00460d

PubMed

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

Abstract

Herein, we report on conductive ultrathin films (nanosheets) with the characteristics of stretchability and water vapor permeability for skin-conformable bioelectrodes. The films are fabricated by combining conductive fibrous networks of single-wall carbon nanotubes (SWCNTs) and poly(styrene-b-butadiene-b-styrene) (SBS) nanosheets (i.e., SWCNT-SBS nanosheets). An increase in the number of SWCNT coatings increases both the thicknesses and densities of the SWCNT bundles. The SBS nanosheets coated with three layers of SWCNTs (i.e., SWCNT 3rd-SBS nanosheets) show comparable sheet resistance to the SBS nanosheets coated with poly(3,4-ethylenedioxithiophene) doped with poly(4-styrenesulfonate acid) (PEDOT:PSS) containing 5 wt.% butylene glycol (i.e., PEDOT:PSS/BG5-SBS nanosheets). In addition, the SWCNT 3rd-SBS nanosheets exhibit significantly reduced elastic moduli and increased elongations at break compared to the PEDOT:PSS/BG5-SBS nanosheets. Furthermore, the calculated water vapor transmission ratio of the 210-nm-thick SBS nanosheets (268,172 g m⁻² (2 h)⁻¹) is greater than that of the filter paper (6345 g m⁻² (2 h)⁻¹). The SWCNT 3rd-SBS nanosheets attached to model skin show high tolerances to bending and artificial sweat at different pH values (i.e., the electrical resistance changes ~1.1 times). Finally, the SWCNT 3rd-SBS nanosheet is applied to detect the surface electromyogram from the forearm of a subject. This nanosheet displays a signal-to-noise ratio similar to that of the PEDOT:PSS/BG5-SBS nanosheet.

DOI： 10.1038/s41427-024-00553-9

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Other Link： https://www.nature.com/articles/s41427-024-00553-9

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/admt.202400487

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Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Elsevier BV  

DOI： 10.1016/j.pdpdt.2024.104167

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

Abstract

Heart disease-related deaths have increased in recent decades, with most patients dying of sudden cardiac arrest. In such instances, the effect of regular electrocardiogram (ECG) measurements is minimal. Therefore, long-term ECG monitoring has become increasingly important. In this paper, we report a non-adhesive high accuracy ECG monitoring system that can be used in various scenarios without interfering with daily activities. The ECG ultra-thin film electrode is made by water-resistant material based on poly(3,4-ethylenedioxythiophene) poly(4-styrenesulfonate) (PEDOT: PSS) electrode doped with ethylene glycol (EG) and xylitol, to improve the noise signal caused by sweat. The optimal ratio of the three ingredients of PEDOT: PSS/xylitol/EG was determined experimentally to accommodate the ECG monitoring. By using the proposed selectively closed multi-channel single-lead logic circuit, the noise of ECG signal received from the proposed film electrode can be successfully reduced during broad-area electrode measurements, thus to improve ECG measurement accuracy.

DOI： 10.1186/s11671-024-03988-9

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Other Link： https://link.springer.com/article/10.1186/s11671-024-03988-9/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsami.3c16134

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

DOI： 10.1021/acsami.3c19320

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：IOP Publishing  

Abstract

Fabrication of a biohybrid actuator requires muscle cells anisotropically aligned in a line, curve, or combination of lines and curves (similar to the microstructure of living muscle tissue) to replicate lifelike movements, in addition to considering the arrangement of skeletal structure or muscular structure with anisotropic straight patterns. Here, we report a UV laser-processed microstructure for freely directing cellular alignment to engineer a biohybrid actuator composed of poly(styrene-block-butadiene-block-styrene triblock copolymer) (SBS) thin film with tailor-made microgrooves (MGs) and skeletal myotubes aligned along these MGs. Specifically, straight, circular, or curved MGs were transferred to SBS thin films from a UV laser-processed template, allowing for the successful alignment of myotubes along MGs. The biohybrid actuator, composed of anisotropically aligned myotubes on a curved microgrooved SBS thin film, was contracted by electrical stimulation. Contraction of biohybrid actuators with curved aligned myotubes permits twisted-like behavior, unlike straight microgrooved films. Therefore, the UV laser-ablation system is a unique maskless and rapid microfabrication technique that provides intriguing opportunities for omni-directional microgrooved structures to achieve the complex motion of living organisms.

DOI： 10.1088/1758-5090/ad2080

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Other Link： https://iopscience.iop.org/article/10.1088/1758-5090/ad2080/pdf

Language：English   Publishing type：Part of collection (book)   Publisher：Springer Japan  

DOI： 10.1007/978-4-431-56936-7_6

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Neural electrodes for electrocorticography (ECoG) recording and electrical stimulation are used for diagnosis and treatment of epilepsy, which are temporarily attached to the surface of cerebral cortex within the subdural space. However, conventional electrodes have a mechanical mismatch with the surface curvature of the brain cortex. Thus, it is necessary to develop a thin and flexible ECoG electrode to overcome the mechanical mismatch with cortex tissue and achieve long‐term ECoG measurement. Herein, a flexible thin‐film electrode is reported with a thickness of ≈8 µm by exploiting printed electronics. A simple fabrication method of conductive wiring is proposed by inkjet printing Au nanoink on a flexible elastomeric thin film consisting of polystyrene‐block‐polybutadiene‐block‐polystyrene (SBS). The conductive wiring printed on the SBS thin film is encapsulated by another SBS thin film as an insulation layer, with laser‐processed micropatterns. The flexible thin‐film electrode is implanted on the cortical surface of rats and used for in vivo ECoG recording and electrical stimulation without the occurrence of severe inflammatory reactions after 6 weeks of implantation. The flexible electrode with 35 ECoG channels visualized the epileptiform activity in a drug‐induced epilepsy rat model, which demonstrates the potential application for the management of intractable epilepsy.

DOI： 10.1002/admt.202300300

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Publisher：Cold Spring Harbor Laboratory  

The widespread use of fluorescence lifetime imaging microscopy (FLIM) for quantitative imaging is hindered by the limited availability of a FLIM-based genetically encoded indicator using a conventional 488 nm laser. Here, we present qMaLioffG, a single green fluorescent protein FLIM indicator showing a fluorescence lifetime change in ATP concentration within the physiological range. This allows quantitative imaging of endogenous ATP to investigate cellular energy status of different cell types.

DOI： 10.1101/2023.08.29.555275

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Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：AHFE International  

We developed a simulator to practice the digital disimpaction technique, a kind of excretory care that is commonly performed by home-care nurses in Japan. The key point of this study is that the requirements for creating a simulation model were not known in advance, so we adopted a rapid prototyping method, in which product development is carried out by repeatedly creating and testing simple prototypes and a user-participatory design method, where feedback is obtained directly from users. We proceeded with the development of the simulator and the definition of requirements simultaneously, repeating the process of creation, trial use, and feedback from interviews with skilled nurses. Digital disimpaction is a complex procedure involving interaction with a living body, in which a finger stimulates the rectum and facilitates the excretion of feces leveraging the rectum's natural biological response. Digital disimpaction is performed primarily by a nurse. Since digital disimpaction is an embarrassing and sometimes painful procedure for the patient, a smooth and comfortable technique is desirable. On the other hand, only a limited set of circumstances are provided to learn the digital disimpaction technique. The requirement for a "procedure requiring non-visible interaction," such as digital disimpaction, is hard to acquire through a simple interview because it is performed based on subjective sensation. Further, while the movement of the finger within the body is crucial, it is essentially impossible to learn through observation of a skilled nurse as it is not visible from the outside. For this reason, we created a simple rectal model as a first prototype. We improved the model by extracting requirements from observations and feedback on actual trial use by skilled nurses. From the first user experience of the simple 3D printed model, requirements were extracted regarding the texture of the rectum, the interaction between finger movement and rectal response, and the characteristics of the feces. A rectal model with a mucosal structure and a fecal model was created based on the obtained requirements. Through the second user experience, the process was repeated, where requirements were again extracted regarding the shape of the rectal model, the characteristics of the mucosa, and the rectal response with the finger movement. We redesigned the model to meet these refined requirements and developed a buttock model covering the rectal model.The results of the third user experience confirmed that the simulator mostly reproduced the intestinal setting as perceived by the nurses during the actual digital disimpaction procedure. This study suggests that the combination of rapid prototyping and user-participatory design can be used to systemize subjects for which requirements are hard to define in advance, such as care procedures based on tacit knowledge.

DOI： 10.54941/ahfe1004131

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.bbrc.2023.05.032

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Molecularly imprinted polymers (MIPs) are synthetic polymers with specific binding sites that present high affinity and spatial and chemical complementarities to a targeted analyte. They mimic the molecular recognition seen naturally in the antibody/antigen complementarity. Because of their specificity, MIPs can be included in sensors as a recognition element coupled to a transducer part that converts the interaction of MIP/analyte into a quantifiable signal. Such sensors have important applications in the biomedical field in diagnosis and drug discovery, and are a necessary complement of tissue engineering for analyzing the functionalities of the engineered tissues. Therefore, in this review, we provide an overview of MIP sensors that have been used for the detection of skeletal- and cardiac-muscle-related analytes. We organized this review by targeted analytes in alphabetical order. Thus, after an introduction to the fabrication of MIPs, we highlight different types of MIP sensors with an emphasis on recent works and show their great diversity, their fabrication, their linear range for a given analyte, their limit of detection (LOD), specificity, and reproducibility. We conclude the review with future developments and perspectives.

DOI： 10.3390/s23125625

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Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.sna.2023.114317

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

Three dimensional (3D) bioprinting is a powerful tool, that was recently applied to tissue engineering. This technique allows the precise deposition of cells encapsulated in supportive bioinks to fabricate complex scaffolds, which are used to repair targeted tissues. Here, we review the recent developments in the application of 3D bioprinting to dental tissue engineering. These tissues, including teeth, periodontal ligament, alveolar bones, and dental pulp, present cell types and mechanical properties with great heterogeneity, which is challenging to reproduce in vitro. After highlighting the different bioprinting methods used in regenerative dentistry, we reviewed the great variety of bioink formulations and their effects on cells, which have been established to support the development of these tissues. We discussed the different advances achieved in the fabrication of each dental tissue to provide an overview of the current state of the methods. We conclude with the remaining challenges and future needs.

DOI： 10.3389/fbioe.2023.991821

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.35848/1882-0786/acc005

Web of Science

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Other Link： https://iopscience.iop.org/article/10.35848/1882-0786/acc005/pdf

Publishing type：Research paper (international conference proceedings)  

Scopus

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

Debonding-on-demand adhesives were designed by fabricating photochemical reversible bonds and using dynamic polymer cleavage and flow generation mechanisms.

DOI： 10.1039/d2ma01048h

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Publishing type：Research paper (scientific journal)  

DOI： 10.1002/admt.202101486

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Language：English   Publishing type：Research paper (international conference proceedings)  

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Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Abstract

Polymer thin‐film sensors have attracted considerable attention in various applications owing to their highly transparent, flexible, and gas‐permeable features. However, conventional thin‐film sensors based on nanomaterials suffer from poor selectivity in sensing targets and scalability of functions. Therefore, a new approach is required for achieving higher selectivity with simple processibility. This study proposes highly transparent, ultra‐flexible, and gas‐permeable polymer thin‐film sensors using ion gels as the sensing material; the sensors demonstrated the capacity for selective detections. Particularly, this study demonstrates simultaneous and independent sensing of temperature and humidity as a proof of concept. The sensors are fabricated using a simple spray coating method on a thin silicone rubber film (≈25 µm thickness). Owing to their thin‐film shape, the sensors exhibit more than 80% visible light transmittance and a higher gas permeability than the human transepidermal water loss. The temperature and humidity are simultaneously detected with a high sensitivity of 15.4% °C^–1 and 2.0% per percentage of the relative humidity, respectively, using gels containing two different ionic liquids (ILs). The results suggest that the easily modifiable nature of ILs enables the fabrication of ultra‐flexible and transparent sensors that can detect various objects using a simple method.

DOI： 10.1002/admt.202200209

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/admt.202200209

Language：English   Publishing type：Research paper (scientific journal)  

Stem cell transplantation is expected to be an effective treatment for intractable skin ulcers by promoting angiogenesis; however, it is challenging to quickly realize a sufficient bloodstream for the ulcers. For this treatment, sheet-like materials with monolayer cells such as cell sheets have been investigated. However, they have a limitation of cell number that can be transplanted at one time due to the two-dimensional, monolayer cell structure, and sufficient secretion of growth factors cannot be expected. In this regard, cellular aggregates, such as spheroids, can reproduce three-dimensional cell-cell interactions that cause biological functions of living tissues more representative than monolayer cells, which is important to achieving efficient secretion of growth factors. In this study, we focused on free-standing porous polymer ultrathin films ("porous nanosheets") comprising poly(d,l-lactic acid) (PDLLA) and succeeded in developing a spheroid-covered nanosheet, on which more than 1000 spheroids from adipose-tissue derived stem cells (ASCs) were loaded. The porous structure with an average pore diameter of 4 μm allowed for facile filtration and carrying spheroids on the nanosheet, as well as sufficient oxygen and nutrients inflow to the cells. The spheroid-covered nanosheet achieved homogeneous transference of spheroids to a whole skin defect in diabetic model mice. Given the continuous release of vascular endothelial growth factor (VEGF) from the spheroids, the transplanted spheroids promoted healing with more accelerated angiogenesis than a nanosheet with a monolayer of cells. The spheroid-covered nanosheet may be a new regenerative material for promoting intractable skin ulcer healing.

DOI： 10.1002/jbm.b.34995

PubMed

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Publishing type：Research paper (scientific journal)   Publisher：IOP Publishing  

<title>Abstract</title>
To increase safety and reduce the electric circuit cost, dielectric elastomer actuators (DEAs) must operate below the kV range. The simplest strategy to reduce the voltage operation is to decrease the dielectric elastomer membrane thickness. This research aims to demonstrate DEAs with a nanometric uniform thickness that can operate at a low voltage (below 70 V) and a high frequency. We use the roll-to-roll process to fabricate a 600 nm thick stretchable PDMS (polydimethylsiloxane) nanosheet and a 200 nm thick conductive nanosheet. These nanosheet-DEAs are tested in high-frequency operations of DC voltage below 70 V and in a frequency range of 1–30 kHz.

DOI： 10.35848/1882-0786/ac3d41

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Other Link： https://iopscience.iop.org/article/10.35848/1882-0786/ac3d41/pdf

Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

Neurotransmitters, which are responsible for the signal transduction of nerve cells in the brain, are linked not only to various emotions and behaviors in our daily life, but also to brain diseases. Measuring neurotransmitters in the brain therefore makes a significant contribution to the progress of brain science. The purpose of this study is to develop a flexible thin film-type sensor that can electrochemically measure dopamine (DA) selectively and with high sensitivity. The thin-film sensor was prepared by printing gold colloidal ink on a polyimide film with a thickness of 25 µm—which the most flexible of the films examined that could maintain the buckling load (1 mN) required for insertion into the brain. The electrode (DA-PPy electrode) was then prepared by electropolymerization of polypyrrole (PPy) using DA as a template. The flexural rigidity of the sensor was 4.3 × 10³ nNm, which is the lowest of any neurotransmitter sensors reported to date. When a DA solution (0–50 nM) was measured with the DA-PPy electrode using square-wave voltammetry (SWV), the slope of the calibration curve was 3.3 times higher than that of the PPy only negative control electrode, indicating an improvement in sensitivity by molecular imprinting with DA. The sensor was used to measure 0−50 nM norepinephrine (NE) and serotonin (5-HT), and the slope of the DA calibration curve at 0.24 V (19 ± 4.4 nA/nM) was much greater than those of NE (0.99 ± 3.3 nA/nM) and 5-HT (2.5 ± 2.4 nA/nM) because the selectivity for DA was also improved by molecular imprinting.

DOI： 10.3389/fsens.2021.725427

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：John Wiley and Sons Inc  

DOI： 10.1002/admi.202100213

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DOI： 10.1002/adfm.202102444

Scopus

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.5104/jiep.24.353

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Language：English   Publishing type：Research paper (scientific journal)  

The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technical restrictions for transplantation of spheroids in clinical practice. Here, we show an effective method for transplantation of ASC spheroids onto skin wounds in order to efficiently cure refractory ulcers. To assist anchoring of spheroids onto skin wounds, we used a 120-nm-thick free-standing film (nanosheet) that has a highly adhesive property. Bioluminescence imaging showed that ASC spheroids carried by the nanosheet survived for 14 days, which is about two-times longer than that previously reported. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14. This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds.

DOI： 10.1038/s41598-021-93642-6

PubMed

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Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

<p>The rapid detection of postprandial hyperglycaemia is imperative for the diagnosis of diabetes and the assessment of health risks for nondiabetics.</p>

DOI： 10.1039/d1tc00667c

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Language：English   Publishing type：Research paper (scientific journal)  

Metronomic photodynamic therapy (mPDT) is a form of PDT that induces cancer cell death by intermittent continuous irradiation with a relatively weak power of light for a long duration (several days). We previously developed a wirelessly powered, fully implantable LED device and reported a significant anti-tumor effect of mPDT. Considering application in clinical practice, the method used for repeated administrations of photosensitizers required for mPDT should not have a high patient burden such as the burden of transvenous administration. Therefore, in this study, we selected 5-aminolevulinic acid (ALA), which can be administered orally, as a photosensitizer, and we studied the antitumor effects of mPDT. In mice with intradermal tumors that were orally administered ALA (200 mg/kg daily for 5 days), the tumor in each mouse was simultaneously irradiated (8 h/day for 5 days) using a wirelessly powered implantable green LED device (532 nm, 0.05 mW). Tumor growth in the mPDT-treated mice was suppressed by about half compared to that in untreated mice. The results showed that mPDT using the wirelessly powered implantable LED device exerted an antitumor effect even with the use of orally administered ALA, and this treatment scheme can reduce the burden of photosensitizer administration for a patient.

DOI： 10.1038/s41598-020-79067-7

PubMed

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Language：English   Publishing type：Research paper (international conference proceedings)  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley-VCH Verlag  

DOI： 10.1002/adbi.201900306

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/adom.201902074

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/adom.201902074

Language：English  

Synthetic biodegradable polymers including poly(lactic acid) (PLA) are attractive cell culture substrates because their surfaces can be micropatterned to support cell adhesion. The cell adhesion properties of a scaffold mainly depend on its surface chemical and structural features; however, it remains unclear how these characteristics affect the growth and differentiation of cultured cells or their gene expression. In this study, we fabricated two differently structured PLA nanosheets: flat and microgrooved. We assessed the growth and differentiation of mouse primary cultured cortical neurons on these two types of nanosheets after pre-coating with poly-D-lysine and vitronectin. Interestingly, prominent neurite bundles were formed along the grooves on the microgrooved nanosheets, whereas thin and randomly extended neurites were only observed on the flat nanosheets. Comparative RNA sequencing analyses revealed that the expression of genes related to postsynaptic density, dendritic shafts, and asymmetric synapses was significantly and consistently up-regulated in cells cultured on the microgrooved nanosheets when compared with those cultured on the flat nanosheets. These results indicate that microgrooved PLA nanosheets can provide a powerful means of establishing a culture system for the efficient and reproducible differentiation of neurons, which will facilitate future investigations of the molecular mechanisms underlying the pathogenesis of neurological disorders.

DOI： 10.1038/s41598-020-63537-z

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/aelm.201901143

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Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

<p>We investigated a porous nanosheet to induce the formation of spheroids consisting of adipose-tissue derived stem cells, which is useful not only for engineering 3D cellular organization, but also for imaging the detailed structure of the spheroid.</p>

DOI： 10.1039/d0tb00723d

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Nature Research  

DOI： 10.1038/s41427-019-0183-1

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Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：IEEE Computer Society  

DOI： 10.1109/OMN.2019.8925133

Scopus

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publisher：公益社団法人 日本生体医工学会  

汗中に含まれる電解質などの化学物質を連続的に計測することで症状の早期発見を目的とした健康モニタリングデバイスへの関心が高まっている.我々はこれまで皮膚貼付型汗中イオン計測デバイスの開発を目的とし,ISFETを用いた小型軽量な低消費電力回路設計技術を構築してきた.しかし,汗に直接センサを押し当てて測定する場合,不純物の混入や気化によって濃度が高くなるという問題がある.そこで本研究では,マイクロ流路を用いて汗の吸上げから排出までを受動的に行い,その過程でイオンを計測可能な皮膚貼付型電子デバイスの開発を目的とする．上記目的を実現すべく,流路には親水性の高い材料を使用した.また,マイクロ流体は層流であり,電解質などの物質は拡散の影響を受けやすい.よって,吸上げ上部の流路壁面の一部をISFETに置き換え,測定結果に拡散の影響が生じないようにした.上記設計の有用性を検証すべく,人工汗液を流し,溶液の先端位置と経過時間の計測を行った結果,溶液は親水性面による界面張力を受け受動的に流動した.また,溶液の濃度を変化させたところ,濃度変化後,約12秒の遅れは生じるが変化を測定できた.よって,汗イオン濃度計測が有用である可能性が示唆された.

DOI： 10.11239/jsmbe.annual57.s164_2

CiNii Research

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Language：Japanese   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publisher：The Japan Society of Mechanical Engineers  

We use ultrathin polymer film called nanosheet to develop wearable battery-less devices which don’t need adhesive to be attached to the skin. Considering NFC (Near Field Communication) is not suitable for the skin-adhesive devices because the capacitance between the skin and the antenna coil reduces the voltage, we adopt electric NFC to transmit data and power to communicate through the human body. We measured the relationship between the IC tag yield rate and the three factors: the area of the electrodes, the distance between the two electrodes, and the thickness of the nanosheet. All of the 6 devices worked and the electric NFC devices are 79% thinner than the conventional NFC devices. Thus, electric NFC enables nanosheet devices to work without batteries.

DOI： 10.1299/jsmermd.2019.1p2-c06

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publishing type：Research paper (scientific journal)  

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Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)  

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Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033243764

Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033244109

Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033243757

Language：Japanese   Publisher：東京 : 電気学会  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033054700

J-GLOBAL

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J-GLOBAL

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J-GLOBAL

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Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I032694304

Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I032694292

Language：Japanese   Publisher：東京 : 電気学会  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I032353988

J-GLOBAL

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Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I032691932

Language：Japanese   Publisher：[東京] : Institute of Electrical Engineers of Japan  

CiNii Research

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I032694238

Language：Japanese  

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J-GLOBAL

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J-GLOBAL

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J-GLOBAL

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Language：Japanese  

J-GLOBAL

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Language：Japanese  

J-GLOBAL

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J-GLOBAL

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Language：Japanese   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：English   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Oral presentation (general)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：English   Presentation type：Oral presentation (invited, special)  

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Language：English   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Oral presentation (invited, special)  

Venue：SanDiego   Country：United States  

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Language：English   Presentation type：Poster presentation  

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Language：English   Presentation type：Poster presentation  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Poster presentation  

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Language：English   Presentation type：Oral presentation (keynote)  

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Language：Japanese   Presentation type：Oral presentation (keynote)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：English   Presentation type：Oral presentation (invited, special)  

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Language：English   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Presentation type：Oral presentation (invited, special)  

Venue：札幌  

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Presentation type：Oral presentation (invited, special)  

Venue：神戸  

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Presentation type：Public lecture, seminar, tutorial, course, or other speech  

Venue：東京  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Language：Japanese   Presentation type：Oral presentation (invited, special)  

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Venue：Hiroshima  

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Language：English  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Application no：2021177723  Date applied：2021.10

Announcement no：2023066879  Date announced：2023.5

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Application no：PCT/JP2021/025114  Date applied：2021.7

Announcement no：WO/2022/009792  Date announced：2022.1

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Application no：特願PCT/JP2024/019615 

Announcement no：特開WO/2024/257609  Date announced：2024.12

Publication no：WO2024/257609 

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