Updated on 2026/03/05

写真a

 
HORI KOICHI
 
Organization
School of Life Science and Technology Researcher
Title
Researcher
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News & Topics

  • Investigating the Intricacies of Auxin Signaling Mechanisms in Algae

    2023/06/30

    Languages： English

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    Genetic analyses conducted on an alga species may provide clues to the origin of auxin signaling mechanisms, as reported by scientists at Tokyo Tech. A series of experiments on *Klebsormidium nit

  • 藻類から解き明かす陸上植物のオーキシン応答の起源 藻類に陸上植物とは異なるオーキシン情報伝達因子を発見

    2023/06/22

    Languages： Japanese

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    要点-植物ホルモンであるオーキシンの応答を、陸上植物の出現過程で分岐したストレプト藻類の一種で解析。-ストレプト藻類は、陸上植物とは異なる情報伝達因子でオーキシン応答を誘導していることを発見。-陸上植物にとって極めて重要なオーキシン応答の起源解明に期待。

  • 藻類から陸上植物への進化をつなぐ車軸藻植物のゲノム配列を解読 －植物の陸上進出の謎を解明するための大きな道開く－

    2014/05/29

    Languages： Japanese

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    ・藻類と陸上植物の中間的な存在である車軸藻植物門クレブソルミディウムのゲノムを解読し、藻類から陸上植物に至る遺伝子の進化過程を解明・クレブソルミディウムは、藻類でありながら、植物ホルモンや強い光に適応するための遺伝子など、植物の陸上進出に重要と考えられるシステムの一部をすでに獲得していることを示した東京工業大学バイオ研究基盤支援総合センターの堀孝一CREST研究員、地球生命研究所/生命理工学研究科の黒川顕教授、バイオ研究基盤支援総合センター/地球生命研究所の太田 啓之教授、かずさDNA研究所、理化学研究所を含む研究グループは、藻類と陸上植物の中間的な存在である車軸藻植物門「クレブソルミディウム」に着目してゲノム解読を行い、藻類から陸上植物に至る遺伝子の進化過程を解明した。それを他の藻類や陸上植物と比較して、藻類から陸上植物に至る過程でどのように遺伝子が多様化したのかを明らかにした。

  • Genome analysis reveals how algae evolved into land plants

    2014/05/29

    Languages： English

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    By analysing the genome of a terrestrial alga, a research group including researchers at Tokyo Institute of Technology, Kazusa DNA Research Institute and RIKEN reveal the presence of genes that enable plants to cope with the extremes of life on land, such as drought, and exposure to high-intensity light and UV.“The colonisation of land by plants was a key event in the evolution of life,” point out researchers in Japan in a recent report. The transition provided the nutrients and oxygen that allow terrestrial creatures to survive, yet so far there has been little evidence of the stages in the evolution of plants that allowed them to adapt from aquatic life to conditions on land.

Research Areas

  • Life Science / Plant molecular biology and physiology

  • Life Science / Evolutionary biology

  • Life Science / Functional biochemistry

Education

  • The University of Tokyo   Graduate School of Arts and Sciences

    2002.4 - 2005.3

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  • The University of Tokyo   Graduate School of Arts and Sciences

    2000.4 - 2002.3

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  • Yokohama National University   College of Education

    1994.4 - 1998.3

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Research History

  • Tokyo Institute of Technology   School of Life Science and Technology   Researcher

    2024.4

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  • Tokyo Institute of Technology   School of Life Science and Technology   Assistant Professor

    2016.4 - 2024.3

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  • Tokyo Institute of Technology   Graduate School of Bioscience and Biotechnology

    2014.2 - 2016.3

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  • Tokyo Institute of Technology

    2010.2 - 2014.1

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  • Rikkyo University   College of Science

    2006.4 - 2010.2

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  • Japan Society for the Promotion of Science

    2004.4 - 2006.3

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  • Showa University   School of Pharmacy Department of Biological Chemistry

    2000.1 - 2000.3

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  • 株式会社 住友金属バイオサイエンス

    1998.4 - 1999.4

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Professional Memberships

Papers

  • Knockout of an SPX-related gene for polyphosphate synthetase accelerates phosphate-starvation responses in the oleaginous microalga Nannochloropsis oceanica

    Kumiko Okazaki, Koichi Hori, Masako Iwai, Tomokazu Kurita, Shinsuke Shimizu, Seiji Nomura, Fumihiko Saito, Shinichiro Maeda, Akihide Takami, Takashi Yamamoto, Hiroyuki Ohta, Atsushi Sakamoto

    Journal of Experimental Botany   2025.4

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    SPX domain–containing proteins are important for phosphate (Pi) signaling and homeostasis in various eukaryotes. Genomic and transcriptomic analyses of the oleaginous microalga Nannochloropsis oceanica identified four genes encoding SPX family members with distinct domain architectures: SPX (NoSPX1), SPX-VTC (NoSPX2), SPX-EXS (NoSPX3), and SPX-MFS (NoSPX4). These NoSPX genes responded differentially to Pi deprivation, with NoSPX1 and NoSPX2 expression being significantly upregulated. NoSPX1 encodes an essential nuclear protein of unknown function and NoSPX2 encodes a vacuolar or acidocalcisomal protein homologous to the vacuolar transporter chaperone 4 that catalyzes polyphosphate production for vacuolar phosphorus storage. Disruption of NoSPX2 diminished polyphosphate levels and slowed the net uptake of external Pi, confirming its role in polyphosphate biosynthesis and Pi homeostasis. Furthermore, Nospx2 mutant cells accumulated significantly more triacylglycerol and greater biomass and had enlarged lipid droplets under Pi deficiency compared to control cells. Together with these augmented Pi starvation responses, we observed a marked upregulation of genes involved in autophagy and lipid transfer across organellar membranes and the downregulation of photosynthesis-related genes, likely reallocating resources toward lipid biosynthesis. These results suggest that inadequate polyphosphate storage accelerates Pi starvation responses in microalgae, providing potential strategies for enhancing microalgal triacylglycerol accumulation and lipid productivity.

    DOI： 10.1093/jxb/eraf171

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  • Sulfide-responsive transcription control inEscherichia coli

    Koichi Hori, Rajalakshmi Balasubramanian, Shinji Masuda

    2024.11

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    Authorship：Lead author   Publisher：Cold Spring Harbor Laboratory  

    Abstract

    To elucidate the mechanism of large-scale transcriptional changes dependent on sulfide inEscherichia coli, a large scale RNA-sequencing analysis was performed on wild-type and sulfide-responsive transcription factor YgaV deletion mutants grown under three conditions: aerobic, semi-aerobic, and semi-aerobic with sulfide. The resulting dataset from these six conditions was subjected to principal component analysis, which categorized the data into five principal components. Estimation of the typical gene expression regulatory mechanisms in each category suggested the presence of mechanisms that are dependent on sulfide but independent of YgaV, as well as those that depend on YgaV but not on sulfide. In YgaV-dependent transcriptional regulation, YgaV was found to function as both a repressor and an activator. These results support the previous hypothesis that YgaV acts as a global regulator responsible for redox homeostasis.

    DOI： 10.1101/2024.11.04.621979

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  • Monitoring sodium caseinate and whey protein isolate interaction with mild preheat treatment using ultrasound spectroscopy and confocal laser scanning microscopy Reviewed

    Naoko Yuno-Ohta, Yuuka Hoshi, Mashiro Shimazaki, Hiroyuki Ohta, Koichi Hori

    Colloids and Surfaces B: Biointerfaces   241   114016 - 114016   2024.9

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.colsurfb.2024.114016

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  • SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions

    Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima

    Journal of Plant Research   2024.8

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Land plants have evolved many systems to adapt to a wide range of environmental stresses. In seed plants, oligogalactolipid synthesis is involved in tolerance to freezing and dehydration, but it has not been analyzed in non-vascular plants. Here we analyzed trigalactosyldiacylglycerol (TGDG) synthesis in Marchantia polymorpha. TGDG is synthesized by galactolipid: galactolipid galactosyltransferase [GGGT; SENSITIVE TO FREEZING2 (SFR2) in Arabidopsis]. We analyzed the subcellular localization and GGGT activity of two M. polymorpha SFR2 homologs (MpGGGT1 and MpGGGT2, each as a GFP-fusion protein) using a transient expression system in Nicotiana benthamiana leaves and found that MpGGGT1-GFP localized in the chloroplast envelope membrane. We produced mutants Mpgggt1 and Mpgggt2 and found that TGDG did not accumulate in Mpgggt1 upon treatment of the thallus with acetic acid. Moreover, growth of Mpgggt1 mutants was impaired by acetic acid treatment. Microscopy revealed that the acetic acid treatment of M. polymorpha plants damaged intracellular membranes. The fact that the effect was similar for wild-type and Mpgggt1 plants suggested that MpGGGT has a role in recovery from damage. These results indicate that MpGGGT plays a crucial role in M. polymorpha growth under conditions of acid stress, which may have been encountered during the ancient terrestrial colonization of plants.

    DOI： 10.1007/s10265-024-01564-x

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    Other Link： https://link.springer.com/article/10.1007/s10265-024-01564-x/fulltext.html

  • Phosphatidic acid phosphohydrolase modulates glycerolipid synthesis in Marchantia polymorpha and is crucial for growth under both nutrient-replete and -deficient conditions Reviewed

    Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima

    Planta   258 ( 5 )   92   2023.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Main conclusion

    The phosphatidic acid phosphohydrolase of Marchantia polymorpha modulates plastid glycolipid synthesis through the ER pathway and is essential for normal plant development regardless of nutrient availability.

    Abstract

    Membrane lipid remodeling is one of the strategies plant cells use to secure inorganic phosphate (Pi) for plant growth, but many aspects of the molecular mechanism and its regulation remain unclear. Here we analyzed membrane lipid remodeling using a non-vascular plant, Marchantia polymorpha. The lipid composition and fatty acid profile during Pi starvation in M. polymorpha revealed a decrease in phospholipids and an increase in both galactolipids and betaine lipids. In Arabidopsis thaliana, phosphatidic acid phosphohydrolase (PAH) is involved in phospholipid degradation and is crucial for tolerance to both Pi and nitrogen starvation. We produced two M. polymorpha PAH (MpPAH) knockout mutants (Mppah-1 and Mppah-2) and found that, unlike Arabidopsis mutants, Mppah impaired plant growth with shorter rhizoids compared with wild-type plants even under nutrient-replete conditions. Mutation of MpPAH did not significantly affect the mole percent of each glycerolipid among total membrane glycerolipids from whole plants under both Pi-replete and Pi-deficient conditions. However, the fatty acid composition of monogalactosyldiacylglycerol indicated that the amount of plastid glycolipids produced through the endoplasmic reticulum pathway was suppressed in Mppah mutants. Phospholipids accumulated in the mutants under N starvation. These results reveal that MpPAH modulates plastid glycolipid synthesis through the endoplasmic reticulum pathway more so than what has been observed for Arabidopsis PAH; moreover, unlike Arabidopsis, MpPAH is crucial for M. polymorpha growth regardless of nutrient availability.

    DOI： 10.1007/s00425-023-04247-4

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    Other Link： https://link.springer.com/article/10.1007/s00425-023-04247-4/fulltext.html

  • Cis-regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens Reviewed

    Noriaki Tounosu, Kanami Sesoko, Koichi Hori, Mie Shimojima, Hiroyuki Ohta

    Scientific Reports   13 ( 1 )   2023.6

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    The phytohormone auxin affects numerous processes in land plants. The central auxin signaling machinery, called the nuclear auxin pathway, is mediated by its pivotal receptor named TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFB). The nuclear auxin pathway is widely conserved in land plants, but auxin also accumulates in various algae. Although auxin affects the growth of several algae, the components that mediate auxin signaling have not been identified. We previously reported that exogenous auxin suppresses cell proliferation in the Klebsormidium nitens that is a member of streptophyte algae, a paraphyletic group sharing the common ancestor with land plants. Although K. nitens lacks TIR1/AFB, auxin affects the expression of numerous genes. Thus, elucidation of the mechanism of auxin-inducible gene expression in K. nitens would provide important insights into the evolution of auxin signaling. Here, we show that some motifs are enriched in the promoter sequences of auxin-inducible genes in K. nitens. We also found that the transcription factor KnRAV activates several auxin-inducible genes and directly binds the promoter of KnLBD1, a representative auxin-inducible gene. We propose that KnRAV has the potential to regulate auxin-responsive gene expression in K. nitens.

    DOI： 10.1038/s41598-023-36500-x

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    Other Link： https://www.nature.com/articles/s41598-023-36500-x

  • The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of Escherichia coli Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance Reviewed International journal

    Rajalakshmi Balasubramanian, Koichi Hori, Takayuki Shimizu, Shingo Kasamatsu, Kae Okamura, Kan Tanaka, Hideshi Ihara, Shinji Masuda

    Antioxidants   11 ( 12 )   2022.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    Compositions and activities of bacterial flora in the gastrointestinal tract significantly influence the metabolism, health, and disease of host humans and animals. These enteric bacteria can switch between aerobic and anaerobic growth if oxygen tension becomes limited. Interestingly, the switching mechanism is important for preventing reactive oxygen species (ROS) production and antibiotic tolerance. Studies have also shown that intracellular and extracellular sulfide molecules are involved in this switching control, although the mechanism is not fully clarified. Here, we found that YgaV, a sulfide-responsive transcription factor SqrR/BigR homolog, responded to sulfide compounds in vivo and in vitro to control anaerobic respiratory gene expression. YgaV also responded to H2O2 scavenging in the enteric bacterium Escherichia coli. Although the wild-type (WT) showed increased antibiotic tolerance under H2S-atmospheric conditions, the ygaV mutant did not show such a phenotype. Additionally, antibiotic sensitivity was higher in the mutant than in the WT of both types in the presence and absence of exogenous H2S. These results, therefore, indicated that YgaV-dependent transcriptional regulation was responsible for maintaining redox homeostasis, ROS scavenging, and antibiotic tolerance.

    DOI： 10.3390/antiox11122359

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  • Characterization of the gelation and resulting network of a mixed-protein gel derived from sodium caseinate and ovalbumin in the presence of glucono-δ-lactone. Reviewed

    Yuno-Ohta N, Shimonomura N, Hoshi Y, Leocmach M, Hori K, Ohta H

    Colloids and surfaces. B, Biointerfaces   198   111472 - 111472   2020.11

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.colsurfb.2020.111472

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  • MYB-like transcription factor NoPSR1 is crucial for membrane lipid remodeling under phosphate starvation in the oleaginous microalga Nannochloropsis oceanica. Reviewed

    Murakami H, Kakutani N, Kuroyanagi Y, Iwai M, Hori K, Shimojima M, Ohta H

    FEBS letters   2020.8

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/1873-3468.13902

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  • Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii. Reviewed

    Hidayati NA, Yamada-Oshima Y, Iwai M, Yamano T, Kajikawa M, Sakurai N, Suda K, Sesoko K, Hori K, Obayashi T, Shimojima M, Fukuzawa H, Ohta H

    The Plant journal : for cell and molecular biology   100 ( 3 )   610 - 626   2019.8

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/tpj.14473

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  • SnRK2 protein kinases represent an ancient system in plants for adaptation to a terrestrial environment Reviewed

    Akihisa Shinozawa, Ryoko Otake, Daisuke Takezawa, Taishi Umezawa, Kenji Komatsu, Keisuke Tanaka, Anna Amagai, Shinnosuke Ishikawa, Yurie Hara, Yasuko Kamisugi, Andrew C. Cuming, Koichi Hori, Hiroyuki Ohta, Fuminori Takahashi, Kazuo Shinozaki, Takahisa Hayashi, Teruaki Taji, Yoichi Sakata

    Communications Biology   2 ( 1 )   2019.1

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    The SNF1-related protein kinase 2 (SnRK2) family includes key regulators of osmostress and abscisic acid (ABA) responses in angiosperms and can be classified into three subclasses. Subclass III SnRK2s act in the ABA response while ABA-nonresponsive subclass I SnRK2s are regulated through osmostress. Here we report that an ancient subclass III SnRK2-based signalling module including ABA and an upstream Raf-like kinase (ARK) exclusively protects the moss Physcomitrella patens from drought. Subclass III SnRK2s from both Arabidopsis and from the semiterrestrial alga Klebsormidium nitens, which contains all the components of ABA signalling except ABA receptors, complement Physcomitrella snrk2− mutants, whereas Arabidopsis subclass I SnRK2 cannot. We propose that the earliest land plants developed the ABA/ARK/subclass III SnRK2 signalling module by recruiting ABA to regulate a pre-existing dehydration response and that subsequently a novel subclass I SnRK2 system evolved in vascular plants conferring osmostress protection independently from the ancient system.

    DOI： 10.1038/s42003-019-0281-1

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    Other Link： https://www.nature.com/articles/s42003-019-0281-1.pdf

  • Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons Reviewed

    Alex de Mendoza, Amandine Bonnet, Dulce B. Vargas-Landin, Nanjing Ji, Hongfei Li, Feng Yang, Ling Li, Koichi Hori, Jahnvi Pflueger, Sam Buckberry, Hiroyuki Ohta, Nedeljka Rosic, Pascale Lesage, Senjie Lin, Ryan Lister

    Nature Communications   9 ( 1 )   2018.4

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Transposable elements are in a constant arms race with the silencing mechanisms of their host genomes. One silencing mechanism commonly used by many eukaryotes is dependent on cytosine methylation, a covalent modification of DNA deposited by C5 cytosine methyltransferases (DNMTs). Here, we report how two distantly related eukaryotic lineages, dinoflagellates and charophytes, have independently incorporated DNMTs into the coding regions of distinct retrotransposon classes. Concomitantly, we show that dinoflagellates of the genus Symbiodinium have evolved cytosine methylation patterns unlike any other eukaryote, with most of the genome methylated at CG dinucleotides. Finally, we demonstrate the ability of retrotransposon DNMTs to methylate CGs de novo, suggesting that retrotransposons could self-methylate retrotranscribed DNA. Together, this is an example of how retrotransposons incorporate host-derived genes involved in DNA methylation. In some cases, this event could have implications for the composition and regulation of the host epigenomic environment.

    DOI： 10.1038/s41467-018-03724-9

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    Other Link： https://www.nature.com/articles/s41467-018-03724-9.pdf

  • Betaine Lipid Is Crucial for Adapting to Low Temperature and Phosphate Deficiency in Nannochloropsis. Reviewed

    Murakami H, Nobusawa T, Hori K, Shimojima M, Ohta H

    Plant physiology   177 ( 1 )   181 - 193   2018.3

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    Publishing type：Research paper (scientific journal)   Publisher：American Society of Plant Biologists (ASPB)  

    DOI： 10.1104/pp.17.01573

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  • Differently localized lysophosphatidic acid acyltransferases crucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis. Reviewed

    Nobusawa T, Hori K, Mori H, Kurokawa K, Ohta H

    2017.5

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.13512

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  • Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens. Reviewed International journal

    Ohtaka K, Hori K, Kanno Y, Seo M, Ohta H

    Plant physiology   174 ( 3 )   1621 - 1632   2017.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    The phytohormone auxin regulates many aspects of growth and development in land plants, but the origin and evolution of auxin signaling and response mechanisms remain largely unknown. Indeed, it remains to be investigated whether auxin-related pathways diverged before the emergence of land plants. To address this knowledge deficit, we analyzed auxin responses in the charophyte alga Klebsormidium nitens NIES-2285, whose ancestor diverged from a green algal ancestor during the evolution of land plants. This strain is the same as Klebsormidium flaccidum NIES-2285, for which the draft genome was sequenced in 2014, and was taxonomically reclassified as K. nitens This genome sequence revealed genes involved in auxin responses. Furthermore, the auxin indole-3-acetic acid (IAA) was detected in cultures of K. nitens, but K. nitens lacks the central regulators of the canonical auxin-signaling pathway found in land plants. Exogenous IAA inhibited cell division and cell elongation in K. nitens Inhibitors of auxin biosynthesis and of polar auxin transport also inhibited cell division and elongation. Moreover, exogenous IAA rapidly induced expression of a LATERAL ORGAN BOUNDARIES-DOMAIN transcription factor. These results suggest that K. nitens has acquired the part of the auxin system that regulates transcription and cell growth without the requirement for the central players that govern auxin signaling in land plants.

    DOI： 10.1104/pp.17.00274

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  • Genetics of the Blue Light-Dependent Signal Cascade That Controls Phototaxis in the Cyanobacterium Synechocystis sp. PCC6803. Reviewed

    Sugimoto Y, Nakamura H, Ren S, Hori K, Masuda S

    2017.3

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcw218

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  • Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis. Reviewed

    Shimizu T, Shen J, Fang M, Zhang Y, Hori K, Trinidad JC, Bauer CE, Giedroc DP, Masuda S

    2017.2

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1614133114

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  • Tangled evolutionary processes with commonality and diversity in plastidial glycolipid synthesis in photosynthetic organisms. Reviewed

    Hori K, Nobusawa T, Watanabe T, Madoka Y, Suzuki H, Shibata D, Shimojima M, Ohta H

    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids   1861 ( 9 )   1294 - 1308   2016.9

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bbalip.2016.04.015

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  • Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution. Reviewed

    Kobayashi Y, Takusagawa M, Harada N, Fukao Y, Yamaoka S, Kohchi T, Hori K, Ohta H, Shikanai T, Nishimura Y

    Genome Biology and Evolution   8 ( 1 )   1 - 16   2016.1

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    Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1093/gbe/evv233

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  • Primitive Extracellular Lipid Components on the Surface of the Charophytic Alga Klebsormidium flaccidum and Their Possible Biosynthetic Pathways as Deduced from the Genome Sequence. Reviewed

    Kondo S, Hori K, Sasaki-Sekimoto Y, Kobayashi A, Kato T, Yuno-Ohta N, Nobusawa T, Ohtaka K, Shimojima M, Ohta H

    Frontiers in Plant Science   7   2016

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    DOI： 10.3389/fpls.2016.00952

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  • Biochemical characterization of allene oxide synthases from the liverwort Marchantia polymorpha and green microalgae Klebsormidium flaccidum provides insight into the evolutionary divergence of the plant CYP74 family. Reviewed

    Koeduka T, Ishizaki K, Mwenda CM, Hori K, Sasaki-Sekimoto Y, Ohta H, Kohchi T, Matsui K

    2015.11

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  • Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation-inducible promoter from Chlamydomonas reinhardtii. Reviewed

    Iwai M, Hori K, Sasaki-Sekimoto Y, Shimojima M, Ohta H

    Frontiers in Microbiology   6   2015

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    Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    DOI： 10.3389/fmicb.2015.00912

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  • Cyanobacterial monogalactosyldiacylglycerol-synthesis pathway is involved in normal unsaturation of galactolipids and low-temperature adaptation of Synechocystis sp. PCC 6803. Reviewed

    Yuzawa Y, Shimojima M, Sato R, Mizusawa N, Ikeda K, Suzuki M, Iwai M, Hori K, Wada H, Masuda S, Ohta H

    2014.4

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  • Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation. Reviewed

    Hori K, Maruyama F, Fujisawa T, Togashi T, Yamamoto N, Seo M, Sato S, Yamada T, Mori H, Tajima N, Moriyama T, Ikeuchi M, Watanabe M, Wada H, Kobayashi K, Saito M, Masuda T, Sasaki-Sekimoto Y, Mashiguchi K, Awai K

    Nature Communications   5   2014

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncomms4978

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  • Whole-Genome Sequence of the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Strain W4. Reviewed

    Masuda S, Hori K, Maruyama F, Ren S, Sugimoto S, Yamamoto N, Mori H, Yamada T, Sato S, Tabata S, Ohta H, Kurokawa K

    2013

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  • Domain-level identification and quantification of relative prokaryotic cell abundance in microbial communities by Micro-FTIR spectroscopy. Reviewed

    Igisu M, Takai K, Ueno Y, Nishizawa M, Nunoura T, Hirai M, Kaneko M, Naraoka H, Shimojima M, Hori K, Nakashima S, Ohta H, Maruyama S, Isozaki Y

    Environmental Microbiology Reports   4 ( 1 )   42 - 49   2012.2

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/j.1758-2229.2011.00277.x

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  • [Nonsense-mediated mRNA decay in plants]. Reviewed

    Hori K, Watanabe Y, Tanpakushitsu kakusan, koso. Protein, nucleic acid, enzyme, vo, Suppl

    Protein, nucleic acid and enzyme   54 ( 16 Suppl )   2189 - 2194   2009.12

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    Hori K, Watanabe Y, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2009, vol. 54, no. 16 Suppl, pp. 2189-2194

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    Other Link： http://orcid.org/0000-0002-7139-4903

  • A virus-induced gene silencing approach for the suppression of nicotine content in Nicotiana benthamiana

    Mari Takizawa, Koichi Hori, Koji Inai, Hisabumi Takase, Takashi Hashimoto, Yuichiro Watanabe

    Plant Biotechnology   24 ( 3 )   295 - 300   2007.12

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    Virus vectors have been used to study plant gene function by transiently overexpressing specific gene products, or conversely, silencing specific endogenous gene functions. Previously, we reported the establishment of tobamovirus vectors and its applications. In this study, we observed that tobamovirus vector could drive ectopic expression of green fluorescent protein (GFP) in roots after infecting leaves, indicating successful invasion into underground tissues. Subsequently, we attempted to apply the tobamovirus vector system to suppress the expression of putrescine N-methyltransferase (PMT), which is the first committed key enzyme for the biosynthesis of nicotine and is active in the underground parts of plants. Two or three weeks after inoculation with the vector harboring a partial fragment of PMT cDNA, we observed a reduction in the PMT mRNA level in the root and in the nicotine content of the aerial parts of the plant Nicotina benthamiana. The possibilities and limitations of the virus vector for the analysis of metabolic pathways are discussed.

    DOI： 10.5511/plantbiotechnology.24.295

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    Other Link： https://jlc.jst.go.jp/DN/JALC/00296660728?from=CiNii

  • Production of dengue 2 envelope domain III in plant using TMV-based vector system. Reviewed International journal

    Saejung W, Fujiyama K, Takasaki T, Ito M, Hori K, Malasit P, Watanabe Y, Kurane I, Seki T

    Vaccine   25 ( 36 )   6646 - 54   2007.9

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    The envelope protein of dengue virus is the major protein involved in host cell receptor binding for viral entry and induction of immunity. A gene fragment encoding domain III of the dengue 2 envelope protein (D2EIII, amino acids 298-400) was successfully expressed in Nicotinana benthamiana plant using a tobacco mosaic virus (TMV)-based transient expression system. The N-terminal 5' untranslated region-omega sequence located upstream of D2EIII increased protein production in infected plant tissues. The recombinant protein was reactive with anti-D2EIII polyclonal and anti-His tag antibodies. The intramuscular immunization of mice with D2EIII induced the production of the anti-dengue virus antibody. The induced antibody demonstrated neutralizing activity against dengue type 2 virus. The result indicates that the TMV expression system produces the dengue virus antigen in plant, which possesses appropriate antigenicity and immunogenicity.

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  • Context analysis of termination codons in mRNA that are recognized by plant NMD.

    Hori K, Watanabe Y

    Plant and Cell Physiology   48 ( 7 )   1072 - 1078   2007.7

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  • UPF3 suppresses aberrant spliced mRNA in Arabidopsis. Reviewed

    Hori K, Watanabe Y

    2005.8

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  • Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens

    Hideki Takahashi, Ayano Shimizu, Tsutomu Arie, Syofi Rosmalawati, Sumire Fukushima, Mari Kikuchi, Yasufumi Hikichi, Ayami Kanda, Akiko Takahashi, Akinori Kiba, Kohei Ohnishi, Yuki Ichinose, Fumiko Taguchi, Chihiro Yasuda, Motoichiro Kodama, Mayumi Egusa, Chikara Masuta, Hiroyuki Sawada, Daisuke Shibata, Koichi Hori, Yuichiro Watanabe

    Journal of General Plant Pathology   71 ( 1 )   8 - 22   2005.2

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s10327-004-0168-x

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    Other Link： http://link.springer.com/article/10.1007/s10327-004-0168-x/fulltext.html

  • Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin. Reviewed

    Masuda Y, Shima G, Aiuchi T, Horie M, Hori K, Nakajo S, Kajimoto S, Shibayama-Imazu T, Nakaya K

    2004.10

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  • Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities. Reviewed

    Suzuki M, Kurimoto E, Nirasawa S, Masuda Y, Hori K, Kurihara Y, Shimba N, Kawai M, Suzuki E, Kato K

    2004.8

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  • Expression of amyloid-beta 1-40 and 1-42 peptides in Capsicum annum var. angulosum for oral immunization. Reviewed

    Szabó B, Hori K, Nakajima A, Sasagawa N, Watanabe Y, Ishiura S

    2004.8

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  • Use of an Attenuated Strain of Tobamovirus for Early Detection of Virus-Induced Gene Silencing Reviewed

    Koichi HORI, Mari TAKIZAWA, Yuichiro WATANABE

    Plant Biotechnology   21 ( 2 )   135 - 142   2004

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Cell and Molecular Biology  

    Virus-induced gene silencing (VIGS) can be used to study gene function by mediating sequence-specific mRNA degradation and suppressing the expression of endogenous target genes. We previously demonstrated that the TocJ vector based on the tomato mosaic tobamoviruses (ToMV) was able to multiply, spread systemically and express green fluorescence protein in Solanaceous plants. TocJ harbouring fragments of endogenous genes could induce VIGS of the parental gene expression, but also induced viral infection symptoms. In this study, an attenuated strain of ToMV, L11A, was used to construct a ToMV vector in order to reduce the virus-induced symptoms. This new vector, named LcJ, was able to spread systemically and mediated VIGS of endogenous genes without visible symptoms. We propose that the use of this attenuated strain for the construction of virus vectors is beneficial for the induction of VIGS.

    DOI： 10.5511/plantbiotechnology.21.135

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  • [Posttranscriptional gene silencing in plants]. Reviewed

    Hori K, Kurihara Y, Watanabe Y, Tanpakushitsu kakusan, koso. Protein, nucleic acid, enzyme, vo, no, Suppl

    蛋白質 核酸 酵素   48 ( 4 Suppl )   459 - 468   2003.3

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    Hori K, Kurihara Y, Watanabe Y, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2003, vol. 48, no. 4 Suppl, pp. 459-468

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  • Beta-hydroxyisovalerylshikonin induces apoptosis in human leukemia cells by inhibiting the activity of a polo-like kinase 1 (PLK1). Reviewed

    Masuda Y, Nishida A, Hori K, Hirabayashi T, Kajimoto S, Nakajo S, Kondo T, Asaka M, Nakaya K

    2003.2

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  • Defective tobamovirus movement protein lacking wild-type phosphorylation sites can be complemented by substitutions found in revertants. Reviewed

    Kawakami S, Hori K, Hosokawa D, Okada Y, Watanabe Y

    2003.1

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  • Construction of a Tobamovirus Vector That Can Systemically Spread and Express Foreign Gene Products in Solanaceous Plants. Reviewed

    Koichi HORI, Yuichiro WATANABE

    Plant Biotechnology   20 ( 2 )   129 - 136   2003

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    In present-day biology, a facile gene delivery system is required to study gene function in a high throughput way. The use of plant virus vectors that can introduce foreign or extra endogenous genes into plants attracts much attention. It is advantageous in that researchers need only minimal experience and results can be obtained in a short period of time. Here we describe infectious cDNA clones of the tomato mosaic tobamoviruses (ToMV) that were modified for facile insertion of foreign genes and which retained the ability to multiply in plants. This vector, named TocJ, was able to harbor the GFP gene from Aequorea victoria and systemically spread in some Solanaceous plants. TocJ would be suitable for the overexpression of foreign genes and the analysis of gene functions in various Solanaceous plants.

    DOI： 10.5511/plantbiotechnology.20.129

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Books

  • 植物科学最前線 7:65

    堀孝一、太田啓之（ Role： Joint author車軸藻クレブソルミディウムのゲノムから見た植物の陸上化）

    2016 

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  • 無敵のバイオテクニカルシリーズRNA実験ノート（下）第3章 RNAiを用いた応用解析 5節, 羊土社, p118-125

    堀孝一、渡辺雄一郎（ Role： Joint authorRNAiの植物への応用）

    2009 

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  • Method in Enzymology 449, 165-176. Reviewed

    Hori K, Watanabe Y（ Role： Joint authorIn vivo analysis of plant nonsense-mediated mRNA decay.）

    2009 

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  • 蛋白質 核酸 酵素増刊, 共立出版, mRNAプログラム 多様性と非対称性の獲得戦略p.2189-2194

    堀孝一、渡辺雄一郎（ Role： Joint author植物のナンセンス変異依存mRNA分解(NMD)）

    2008 

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  • RNAの細胞生物学 「蛋白質 核酸 酵素」増刊

    堀孝一, 栗原志夫, 渡辺雄一郎（ Role： Joint author植物での転写後サイレンシング（PTGS）, p459-468）

    2003 

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MISC

  • TALEN-mediated targeted editing of polyphosphate synthetase and lipase genes enhances oil accumulation under phosphorus starvation in oleaginous microalga Nannochloropsisoceanica

    岡崎久美子, 岩井雅子, 栗田朋和, 堀孝一, 下嶋美恵, 前田真一郎, 高見明秀, 山本卓, 山本卓, 太田啓之, 太田啓之, 坂本敦

    日本植物生理学会年会(Web)   66th   2025

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  • Context analysis of termination Codons in mRNA that are recognized by plant NMD (vol 48, pg 1072, 2007)

    Koichi Hori, Yuichiro Watanabe

    PLANT AND CELL PHYSIOLOGY   48 ( 9 )   1391 - 1391   2007.9

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  • The NMD system influences mRNA primary sequences through genome evolution

    Koichi Hori, Yuichiro Watanabe

    PLANT AND CELL PHYSIOLOGY   48   S11 - S11   2007

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    Language：English   Publishing type：Research paper, summary (international conference)  

    Web of Science

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  • Global analysis of targets of NMD in Arabidopsis

    Masato Yoine, Maya Akahori, Koichi Hori, Yuichiro Watanabe, Kenzo Nakamura

    PLANT AND CELL PHYSIOLOGY   48   S11 - S11   2007

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  • (355) Characterization of the Tomato Mosaic Virus Resistance Gene Tm-2^2 from Tomato (GCR267)(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Okubo E., Okamura H., Takizawa M., Hori K., Watanabe Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   280 - 280   2005.8

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Presentations

  • Comparative transcriptome analysis of Klebsormidium nitens NIES-2285 between solid and liquid culture.

    Hori K and Ohta H

    XIX International Botanical Congress  2017 

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    Venue：Shenzhen, China  

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  • 車軸藻クレブソルミディウムの気相，液相培養によるトランスクリプトーム解析

    堀孝一,太田啓之

    第58回日本植物生理学会年会  2017 

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    Venue：鹿児島大学郡元キャンパス  

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  • 車軸藻類クレブソルミディウムのAREB様bZIP転写因子は水ストレスの共通制御因子として働く

    堀孝一, 唐司典明, 瀬底かなみ, 下嶋美恵, 太田啓之

    第62回日本植物生理学会年会  2020 

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    Venue：オンライン開催  

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  • 車軸藻類クレブソルミディウムの乾燥・沈水応答に関与する転写因子の解析

    堀孝一,唐司典明,瀬底かなみ,下嶋美恵,太田啓之

    日本植物学会第84回大会  2020 

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    Venue：オンライン開催  

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  • 車軸藻植物門クレブソルミディウムの気相への環境応答

    堀孝一, 近藤智, 唐司 典明, 大高きぬ香, 渡邊 汀, 太田啓之

    日本植物学会第79回大会  2015 

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    Venue：朱鷺メッセ：新潟コンベンションセンター  

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  • The Klebsormidium Genome Invited

    Hori K, Kondo S, Ohtaka K, Tounosu N, Watanabe T, Kurokawa K, Ohta H

    International Plant & Animal Genome XXIII  2015 

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    Venue：San Diego, CA, USA  

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  • 植物の陸上進出と遺伝子発現ネットワークの変遷 Invited

    堀孝一

    第５回植物RNA研究ネットワークシンポジウム  2015 

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    Venue：東京大学 駒場キャンパス  

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  • Revision of Klebsormidium flaccidum Gene models (version 1.1)

    Hori K, Umetsu J, Mori H, Kurokawa K and Ohta H

    EMBO workshop : New model systems for early land plant evolution (w16-05) Vienna  2016 

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    Venue：Austria  

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  • 車軸藻類クレブソルミディウムの乾燥応答に関与する転写因子の解析

    堀孝一,唐司典明,瀬底かなみ,下嶋美恵,太田啓之

    第61回日本植物生理学会年会  2020 

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    Venue：大阪大学吹田キャンパス（要旨発表による開催）  

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  • 車軸藻類クレブソルミディウムにおけるリン欠乏応答とグルクロノシルジアシルグリセロール合成酵素の解析

    関根伸輔, 堀孝一, 唐司典明, 井原雄太, 清水信介, 下嶋美惠, 太田啓之

    第18回植物脂質シンポジウム  2021.9 

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  • 植物の陸上進出と乾燥ストレス応答機構の発達 Invited

    堀孝一

    第12回日本光合成学会シンポジウム  2022.5 

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  • クレブソルミディウムのオーキシン応答と乾燥ストレス応答 -陸上植物との共通と相違- Invited

    堀 孝一,唐司 典明,瀬底 かなみ,三木 健,下嶋 美恵,太田 啓之

    日本植物学会第87回大会シンポジウム  2023.9 

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  • NMDはゲノム進化の過程でmRNAの一次配列に影響を与えてきた Invited

    堀孝一、関根靖彦

    第４８回日本植物生理学会大会シンポジウム  2007 

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    Venue：愛媛大学城北キャンパス  

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  • 種々の生物のゲノム配列は潜在的なオルガネラへの移行シグナル配列を含む

    堀孝一、関根靖彦

    第４８回日本植物生理学会大会  2007 

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    Venue：愛媛大学  

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  • 核移行遺伝子の機能獲得機構の予測

    堀孝一、相馬亜希子、関根靖彦

    第14回光合成の色素系と反応中心に関するセミナー  2006 

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    Venue：京都大学  

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  • シロイヌナズナUPF3は異常なスプライシングによるmRNAを抑制する

    堀孝一、木崎学、渡辺雄一郎

    第47回日本植物生理学会大会  2006 

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    Venue：筑波大学  

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  • 核移行遺伝子のプロモーター獲得における散在性反復配列の関与の解析

    堀孝一、相馬亜希子、関根靖彦

    第３０回日本分子生物学会年会  2007 

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    Venue：パシフィコ横浜  

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  • ゲノムデータに基づくオルガネラ移行シグナル配列の獲得過程の予測 Invited

    堀孝一、関根靖彦

    第１０回日本進化学会大会ワークショップ  2007 

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    Venue：東京大学 駒場キャンパス  

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  • The prediction of NMD target genes and involvement of UPF3 in NMD in Arabidopsis

    Hori K and Watanabe Y

    Gordon Research Conferences Plant Molecular Biology Jul 18-23  2004 

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    Venue：Holderness School, USA  

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  • 植物におけるRNAサーベイランスシステム

    堀孝一、木崎学、渡辺雄一郎

    新しいRNA/RNPを見つける会  2005 

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    Venue：山形 慶應義塾大学先端生命科学研究所  

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  • シロイヌナズナUPF3は異常なスプライシングによるmRNAを抑制する

    堀孝一、木崎学、渡辺雄一郎

    第7回日本RNA学会年会  2005 

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    Venue：弘前大学  

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  • The NMD system possibly imposed selection pressures on mRNA primary sequences through genome evolution

    Hori K and Watanabe Y

    2006 

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  • NMDはゲノム進化の過程でmRNAの一次構造に影響を与える

    堀孝一、渡辺雄一郎

    第４８回日本植物生理学会大会シンポジウム  2007 

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    Venue：愛媛大学  

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  • 車軸藻綱クレブソルミディウムのゲノム解析と植物の陸上化要因の予測

    堀孝一, .. (41名略)..., 柴田大輔, 神谷勇治, 佐藤直樹, 中村保一, 田畑哲之, 井田茂, 黒川顕, 太田啓之

    第54回日本植物生理学会年会  2013 

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    Venue：岡山大学津島キャンパス  

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  • 車軸藻綱クレブソルミディウムのオルガネラDNA配列から予測される植物の陸上化要因

    堀孝一、丸山史人、佐藤修正、下嶋美恵、増田真二、佐藤直樹、田畑哲之、黒川顕、太田啓之

    第52回日本植物生理学会年会  2011 

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    Venue：東北大学川内北キャンパス（要旨発表による開催）  

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  • 気生藻類クレブソルミディウムの環境変化に対する光合成応答の解析

    堀孝一、丸山史人、佐藤修正、下嶋美恵、増田真二、佐藤直樹、田畑哲之、黒川顕、太田啓之

    第53回日本植物生理学会年会  2012 

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    Venue：京都産業大学  

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  • 車軸藻植物門クレブソルミディウムのゲノム解析から見た植物の陸上進出 Invited

    堀孝一

    第9回光合成若手の会  2013 

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    Venue：東京大学本郷キャンパス  

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  • 車軸藻植物門クレブソルミディウムのゲノム解析と脂質代謝系の進化過程の解析

    堀孝一, .. (41名略)..., 柴田大輔, 神谷勇治, 佐藤直樹, 中村保一, 田畑哲之, 井田茂, 黒川顕, 太田啓之

    日本植物学会第77回大会  2013 

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    Venue：北海道大学  

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  • オルガネラ移行シグナル配列の獲得過程の予測

    堀孝一、関根靖彦

    第４９回日本植物生理学会大会  2008 

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    Venue：札幌コンベンションセンター  

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  • ゲノムデータに基づくN末端シグナル配列の獲得過程の予測

    堀孝一、養老瑛美子、関根康彦

    第５０回植物生理学会年会  2009 

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    Venue：名古屋大学東山キャンパス  

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  • シアノバクテリアにおける葉緑体特異的リボソームタンパク質PSRP-1の機能解析

    堀孝一、長岡敦子、斎藤勝和、前田倫子、黒岩晴子、七宮英晃、福澤淳平、河村富士夫、黒岩常祥、関根靖彦

    第５１回日本植物生理学会年会  2010 

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    Venue：熊本大学 黒髪北キャンパス  

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  • 車軸藻綱クレブソルミディウムのオルガネラDNAの解析と植物の陸上化要因の予測

    堀孝一、丸山史人、佐藤修正、下嶋美恵、増田真二、佐藤直樹、田畑哲之、黒川顕、太田啓之

    日本植物学会第75回大会  2011 

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    Venue：東京大学駒場キャンパス  

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  • ゲノムデータに基づくオルガネラ移行シグナル配列の獲得過程の予測 Invited

    堀孝一, 近藤智, 大高きぬ香, 唐司典明, 渡邊汀, 黒川顕, 太田啓之

    日本植物学会第78回大会シンポジウム「古い酒を新しい革袋に～preexisting gene regulatory networkの転用による陸上植物のボディプラン革新」  2014 

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    Venue：明治大学生田キャンパス  

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  • 車軸藻植物門クレブソルミディウムのゲノム解読と他植物との比較解析

    堀孝一, 山本希, 梅津純平, 富樫智章, 円由香, 下嶋美恵, 瀬尾光範, 近藤智, 大高きぬ香, 唐司 典明, 渡邊 汀, 東光一, 森宙史, 山田拓司, 増田真二, 黒川顕, 太田啓之

    第55回日本植物生理学会年会  2014 

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    Venue：富山大学五福キャンパス  

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  • ジーンサイレンシングの誘導に適したトバモウイルスベクターへの改良

    堀孝一、渡辺雄一郎

    第21回日本植物細胞分子生物学会大会  2003 

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    Venue：香川県県民ホール  

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  • 植物におけるAtUPF3のNonsense mediated decayへの関与

    堀孝一、木崎学、渡辺雄一郎

    第２７回日本分子生物学会年会  2004 

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    Venue：神戸国際会議場  

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  • 植物におけるNMD標的遺伝子の検索とAtUPF3のNMDへの関与

    堀孝一、渡辺雄一郎

    第6回日本RNA学会年会  2004 

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    Venue：熊本テルサ  

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  • ジーンサイレンシングの誘導に適したトバモウイルスベクターへの改良

    堀孝一、渡辺雄一郎

    第20回日本植物細胞分子生物学会大会  2002 

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    Venue：奈良 帝塚山大学  

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  • ジーンサイレンシングの誘導に適したトバモウイルスベクターへの改良

    堀孝一、渡辺雄一郎

    第4回日本RNA学会年会  2002 

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    Venue：つくば国際会議場  

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  • 新規ウイルスベクターの構築

    堀孝一、渡辺雄一郎

    第43回日本植物生理学会年会  2002 

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    Venue：岡山大学  

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  • Application of plant virus vector to analyze RNA silencing in Arabidopsis thaliana.

    Hori K and Watanabe Y

    RNA 2003  2003 

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    Venue：Kyoto International Conference Hall, Kyoto, Japan  

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Industrial property rights

  • Δ４デサチュレースによるドコサヘキサエン酸合成

    太田 啓之, 永井 千夏, 下嶋 美恵, 岩井 雅子, 堀 孝一, 佐々木 結子

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    Applicant：国立大学法人東京工業大学

    Application no：特願2019-207244  Date applied：2019.11

    Announcement no：特開2021-078379  Date announced：2021.5

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  • 微生物及びトリアシルグリセロールの製造方法

    坂本敦, 岡崎久美子, 山本卓, 太田啓之, 堀孝一, 清水信介, 高見明秀, 野村誠治, 斉藤史彦

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    Applicant：国立大学法人広島大学, 国立大学法人東京工業大学, マツダ株式会社

    Application no：特願2019-014800  Date applied：2019.1

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  • 脂質の製造方法

    太田 啓之, 下嶋 美恵, 信澤 岳, 堀 孝一, 佐々木 結子, 岩井 雅子, 永井 千夏

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    Applicant：花王株式会社, 国立大学法人東京工業大学

    Application no：特願2018-116296  Date applied：2018.6

    Announcement no：特開2019-216643  Date announced：2019.12

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  • β－ケトアシル－ＡＣＰシンターゼを用いた脂質の製造方法

    杉原 慎二, 尾崎 達郎, 遠藤 寛子, 斎藤 猛, 東條 卓人, 太田 啓之, 堀 孝一

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    Applicant：花王株式会社

    Application no：特願2016-506427  Date applied：2015.2

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  • アシル－ＡＣＰチオエステラーゼ

    尾崎 達郎, 太田 啓之, 堀 孝一

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    Applicant：花王株式会社

    Application no：特願2014-554412  Date applied：2013.12

    Patent/Registration no：特許第6381139号  Date registered：2014.7 

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Awards

  • 手島精一記念研究賞 研究論文賞

    東京工業大学   Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation

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Research Projects

  • 車軸藻類クレブソルミディウムを用いた、植物の乾燥ストレス情報伝達経路の起源の解明

    2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    堀孝一

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    Authorship：Principal investigator 

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  • 複合系食品ゲルの構造観察と超音波分光分析を用いた物性発現機構の解明

    2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    太田 尚子, 堀 孝一

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    Authorship：Coinvestigator(s) 

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  • 光合成生物に広く保存された栄養欠乏時の脂質転換制御とその応用の分子基盤

    Grant number：18H03941  2018.4 - 2023.3

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    太田 啓之, 増田 建, 西村 浩二, 小林 康一, 堀 孝一, 粟井 光一郎, 下嶋 美恵, 清水 隆之

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    Grant amount：\43810000 （ Direct Cost: \33700000 、 Indirect Cost：\10110000 ）

    生物に必須の栄養元素の欠乏時には種々の応答が起こることが広く知られている。栄養元素の欠乏時には、トランスポーターの活性化などによる細胞外からの栄養元素の取り込みの促進や、栄養元素の細胞内でのリサイクルの活性化など、様々な応答機構が機能する。申請者らは植物の３大栄養元素の一つであるリンの欠乏時に起こる膜リン脂質からのリンの供給と糖脂質による代替を「リン欠乏時の膜脂質転換」と名付け、それに関わる脂質代謝遺伝子群やその重要性を明らかにしてきた。植物や藻類のリン欠乏時には、油脂が大量に蓄積することも見出し、光合成生物での油脂高生産系としても着目されている。また、その膜脂質転換に関わる酵素群や制御機構が光合成生物の進化過程で広く保存されていることも明らかにした。申請者は最近、その制御の根幹に関わると考えられる制御因子を初めて同定することに成功した。本研究では、この制御因子の機能と保存性の解明を柱としてリン欠乏時の脂質転換の仕組みの分子基盤を明らかにし、その応用展開の基盤確立を目的として研究を進めている。
    平成30年度は特に、クラミドモナスで見出した脂質転換の制御因子についてその因子が直接ターゲットとしている遺伝子の同定、また本因子と共同して機能している他のクラミドモナス因子の同定を行った。

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  • クレブソルミディウムのTIR1を介さないオーキシン応答を誘導する転写因子の探索

    Grant number：18K06280  2018.4 - 2021.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    堀 孝一

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

    本研究課題は、陸上植物の主要なオーキシン応答経路を獲得する前段階にある車軸藻植物門のクレブソルミディウムに着目し、クレブソルミディウムのオーキシン一次応答に関与する転写因子の同定と機能解析を行う事により、陸上植物のオーキシン情報伝達経路の出現過程を解明する事が目標である。本年度行った研究の中でも、クレブソルミディウムにおいてオーキシン一次応答に関わる転写因子を探索するために、ベンサミアナタバコを用いたレポーターアッセイによる候補転写因子のスクリーニングを試みた結果、大きな進展が見られた。
    まず、クレブソルミディウムのオーキシン応答性遺伝子のシス配列解析と既知の転写因子結合配列から転写因子候補の絞り込みを行った。その結果、クレブソルミディウムの全遺伝子から推定した、４６種類、約２４０遺伝子の転写因子のうち、３種類１６遺伝子がオーキシン一次応答に関わる候補転写因子として絞り込まれた。この中から偽遺伝子を除いた１１遺伝子をクローニングし、それぞれをクレブソルミディウムのオーキシン誘導性プロモーターと同時にベンサミアナタバコに導入した。クレブソルミディウムのオーキシン誘導性プロモーターは、我々がオーキシン応答遺伝子として同定したKnLBD1プロモーターを用い、レポーターとしてGUS遺伝子を下流に配置した。
    その結果、１１の候補のうちの１つの転写因子が、KnLBD1プロモーターと相互作用してGUS活性を誘導していることが明らかとなった。このことは、この転写因子がクレブソルミディウムのオーキシン応答において一次応答を引き起こす有力な候補であることを意味する。したがって、この転写因子がクレブソルミディウムのオーキシン一次応答に関与する事が実証できれば、その特性を明らかにし、他生物種と比較解析を行うことで陸上植物のオーキシン情報伝達経路の出現過程の議論が可能になると期待できる。

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  • Presence of wax components of Charophytes revealed by Genome and suface lipid analyses and their importance in adaptation to land environment

    Grant number：15H04393  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Ohta Hiroyuki

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    Grant amount：\18070000 （ Direct Cost: \13900000 、 Indirect Cost：\4170000 ）

    In this study, we proposed that charophyte algae, which are the closest algae to land plants, acquired a cuticle-like hydrophobic layer composed of triacylglycerol to adapt to a terrestrial environment by initial land colonization. To demonstrate the hypothesis, we analyzed surface lipids and transcriptome of Klebsormidium, which early diverged from charophyte algae ancestor. As a result, it is assumed that surface structure and its related gene regulation were evolved in a stepwise manner during land colonization while using triacylglycerol as hydrophobic barrier. These results will provide information to clarify relation between process of land colonization by plants and evolution of its surface lipid.

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  • Functional analysis of Plastid-Specific ribosomal Protein

    Grant number：19870023  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (Start-up)  Grant-in-Aid for Young Scientists (Start-up)

    HORI Kouichi

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    Grant amount：\3125000 （ Direct Cost: \2720000 、 Indirect Cost：\405000 ）

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  • 植物の発生、抵抗性反応におけるNMDの機能に関する解析

    Grant number：04J10986  2004 - 2005

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    堀 孝一

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    Grant amount：\1900000 （ Direct Cost: \1900000 ）

    前年までに行ったNonsense mediated mRNA decay (MD)によって分解されている遺伝子(NMD標的遺伝子)の予測とAtUPF3遺伝子のNMDへの関与の結果をまとめ、Plant Journalへの報告を行った。その結果をふまえNMDに関与することが期待されるAtUPF1,AtUPF2とAtUPF3遺伝子の相互作用の解析を、免疫沈降実験によって行った。その結果大腸菌で発現した部分長AtUPF1とAtUPF2はそれぞれ、植物体で発現したAtUPF3と相互作用していることが確認でき、AtUPF1,AtUPF2,AtUPF3は複合体を形成していることが示唆された。次に、AtUPF3と緑色蛍光タンパク質の融合タンパク質の細胞内局在を解析した結果、AtUPF3は核小体および細胞質全般と細胞質に顆粒状の構造として局在していることを明らかにした。さらに3種類のAtUPF3の部分欠失体を作成し、同様に細胞内局在の解析を行った結果、AtUPF3のC末端側2/3が核小体への局在に強く関連していることを示した。またAtUPF3として機能しないことが予測される部分欠失体では全て細胞質の顆粒状の局在が観察されなかったことから、この細胞質における顆粒状構造はNMDにおいて重要な働きをしていることが示唆された。この顆粒状構造はサイクロヘキシミドによってタンパク質翻訳を停止しNMDを阻害すると、すみやかに消失することを発見した。よってこの顆粒状構造はNMDにおけるRNA分解の場であることが期待される。

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Teaching Experience

  • 生命理工学特別講義3 各年で１日 分担

    2019 Institution：東京工業大学

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  • 生命理工学基礎実験・演習第三 A,B,C,D 分担

    2017 Institution：東京工業大学

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Social Activities

  • 神奈川県立厚木高等学校 高校生実験教室

    2015 - 2021

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    Type：Other

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Academic Activities

  • 第12回日本光合成学会年会およびシンポジウム 世話人メンバー

    日本光合成学会  2022

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  • The 23rd International Symposium on Plant Lipids (ISPL2018) 大会準備委員

    植物脂質研究会  2018.7

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    Type：Competition, symposium, etc. 

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  • 日本進化学会第18回大会委員会 大会準備委員

    日本進化学会  2016.8

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    Type：Competition, symposium, etc. 

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  • 日本植物学会台78回大会 大会実行委員会 実行委員

    日本植物学会  2014.9

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    Type：Competition, symposium, etc. 

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