Updated on 2025/07/08

写真a

 
HAYASHI NOBUHIRO
 
Organization
School of Life Science and Technology Professor
Title
Professor
External link

Degree

  • Ph.D. ( Tokyo Institute of Technology )

Research Interests

  • Proteomics

  • micro domain

  • fatty acylation

  • 抗体

  • シグナル伝達系

  • 飽和脂肪酸修飾

  • Protein Science

  • タンパク質科学

Research Areas

  • Natural Science / Biophysics, chemical physics and soft matter physics

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

  • Life Science / Molecular biology

Education

  • Tokyo Institute of Technology   Interdisciplinary Science and Engineering

    - 1994

      More details

    Country: Japan

    researchmap

  • Tokyo Institute of Technology   Graduate School, Division of Integrated Science and Engineering

    - 1994

      More details

  • The University of Tokyo   Faculty of Science

    - 1989

      More details

  • The University of Tokyo   Faculty of Science

    - 1989

      More details

    Country: Japan

    researchmap

Professional Memberships

▼display all

Papers

  • A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli Reviewed

    Akiyoshi Ikeda-Boku, Satoshi Ohno, Yuuka Hibino, Takashi Yokogawa, Nobuhiro Hayashi, Kazuya Nishikawa

    Journal of Biochemistry   153 ( 3 )   317 - 326   2013.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvs153

    Scopus

    PubMed

    researchmap

  • Atg9 Vesicles Recruit Vesicle-tethering Proteins Trs85 and Ypt1 to the Autophagosome Formation Site Reviewed

    Soichiro Kakuta, Hayashi Yamamoto, Lumi Negishi, Chika Kondo-Kakuta, Nobuhiro Hayashi, Yoshinori Ohsumi

    JOURNAL OF BIOLOGICAL CHEMISTRY   287 ( 53 )   44261 - 44269   2012.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M112.411454

    Web of Science

    PubMed

    researchmap

  • NMR protein structure determination in living E. coli cells using nonlinear sampling Reviewed

    Nature Protocols   5 ( 6 )   1051 - 1060   2010.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nprot.2010.69

    researchmap

  • Protein structure determination in living cells by in-cell NMR spectroscopy Reviewed

    Daisuke Sakakibara, Atsuko Sasaki, Teppei Ikeya, Junpei Hamatsu, Tomomi Hanashima, Masaki Mishima, Masatoshi Yoshimasu, Nobuhiro Hayashi, Tsutomu Mikawa, Markus Waelchli, Brian O. Smith, Masahiro Shirakawa, Peter Guentert, Yutaka Ito

    NATURE   458 ( 7234 )   102 - U10   2009.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    DOI: 10.1038/nature07814

    researchmap

  • 3P308 Functional analyses of N-terminal myristoylation of Src using site-directed-labeling method(Bioimaging,Poster Presentations)

    Kondow Akiko, Hagihara Hideo, Yagawa Mizuho, Nakamura Masashi, Ohno Satoshi, Yokogawa Takashi, Mitsuoka Yumiko, Hosoya Takamitsu, Hiramatsu Toshiyuki, Suzuki Masaaki, Hashimoto Keiichiro, Nishikawa Kazuya, Hayashi Nobuhiro

    Seibutsu Butsuri   47   S280   2007

     More details

    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.47.S280_1

    researchmap

  • Direct involvement of protein myristoylation in myristoylated alanine-rich C kinase substrate. Reviewed

    Matsubara, M, Titani, K, Taniguchi, H, Hayashi, N

    J. Biol. Chem.   278 ( 49 )   48898 - 48902   2003

  • Identification of the calmodulin-binding domain of neuron-specific protein kinase C substrate protein CAP-22/NAP-22 - Direct involvement of protein myristoylation in calmodulin-target protein interaction Reviewed

    A Takasaki, N Hayashi, M Matsubara, E Yamauchi, H Taniguchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   274 ( 17 )   11848 - 11853   1999.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Web of Science

    researchmap

  • 脳特異的タンパク質Neuronal tissue-enriched acidic protein-22kDaのカルモジュリン結合ドメインの同定とそのミリスチル化による機能調節の解析 Reviewed

    高崎昭彦, 林宣宏, 松原守, 千谷晃一, 谷口寿章

    藤田学園医学会誌   21   219 - 222   1997

     More details

  • Involvement of basic amphiphilic α-helical domain in the reversible proteins: structural studies by mass spectrometry, circular dichroism, and nuclear magnetic reasonance. Reviewed

    Hayashi, N, Matsubara, M, Titani, K, Taniguchi, H

    In "Techiniques in Protein Chemistry VIII" (D.R.Marchak, ed)   555 - 564   1997

  • Recognition of the anticodon loop of tRNA1/(Ile) by isoleucyl-tRNA synthetase from Escherichia coli Reviewed

    Tatsuya Niimi, Osamu Nureki, Takashi Yokogawa, Nobuhiro Hayashi, Kazuya Nishikawa, Kimitsuna Watanabe, Shigeyuki Yokoyama

    Nucleosides and Nucleotides   13   1231 - 1237   1994.1

     More details

    For Escherichia coli tRNA1/(Ile) having anticodon G34-A35-U36, two variants with substitution and/or insertion in the anticodon loop were prepared by in vitro recombinant RNA method. A variant with replacement of the N-((9-β-D-ribofuranosyl-purine-6-yl)carbamoyl)threonine (t6A) residue at position 37 with an unmodified adenosine exhibited a drastic reduction in isoleucine-accepting activity. This shows that t6A37 plays a crucial role in the recognition by isoleucyl-tRNA synthetase (IleRS). Into this A37 variant, unmodified A was further inserted at position 36 so that a sequence of GAAUA was created. This insertion did not show further reduction in isoleucine- accepting activity, indicating that IleRS recognizes the three anticodon residues, which have already been found to be identity elements, individually but not as a whole.

    researchmap

  • Tertiary structural analysis of Escherichia coli lysine tRNA. Reviewed

    Hayashi N, Kawai G, Takayanagi M, Noguchi T, Ueda T, Nishikawa K, Miura K, Miyazawa T, Yokoyama S, Watanabe K

    Nucleic acids symposium series   ( 29 )   195 - 196   1993

  • Tertiary structures of mitochondrial tRNAs having characteristic secondary structures Reviewed

    Watanabe Y, Kawai G, Yokogawa T, Hayashi I, Hayashi N, Nishikawa K, Ueda T, Watanabe K

    Nucleic Acids Symp Ser   ( 29 )   209 - 10   1993

▼display all

Books

  • Involvement of Basic Amphiphilic α-helicul Domain in the Reversible Membrane Interaction of Amphitropic Proteins : Structural Studies by Mass Spectrometry, Circular Dichroism, and Nucleur Magnetic Resonance(jointly worked)

    Techniques in Protein Chemistry VIII(Academic Press)  1997 

     More details

MISC

  • ノンコーディングRNA研究から発見された新規タンパク質遺伝子AGD3の機能解析

    福田牧葉, 伊藤智哉, 渡部暁, 木川隆則, 林宣宏, 中川裕之, 相澤康則

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   13th   2010

  • Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo

    M Matsubara, J Tao, K Kawamura, N Shimojo, K Titani, K Hashimoto, N Hayashi

    PROTEIN SCIENCE   14 ( 2 )   494 - 503   2005.2

     More details

  • ラクダ抗leucyl-tRNA synthetase抗体の機能および構造の解析 Reviewed

    合田 正貴, 勝見 治恵, 大野 敏, 横川 隆志, 西川 一八, 橋本 敬一郎, 黒澤 良和, 林 宣宏

    生化学   76 ( 10 )   1392 - 1392   2004.10

     More details

    Language:Japanese   Publisher:(公社)日本生化学会  

    researchmap

  • Myristoylation-regulated direct interaction between calcium-bound calmodulin and N-terminal region of pp60(v-src)

    N Hayashi, C Nakagawa, Y Ito, A Takasaki, Y Jinbo, Y Yamakawa, K Titani, K Hashimoto, Y Izumi, N Matsushima

    JOURNAL OF MOLECULAR BIOLOGY   338 ( 1 )   169 - 180   2004.4

     More details

  • MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins

    S Maurer-Stroh, M Gouda, M Novatchkova, A Schleiffer, G Schneider, FL Sirota, M Wildpaner, N Hayashi, F Eisenhaber

    GENOME BIOLOGY   5 ( 3 )   2004

     More details

    Language:English   Publishing type:Book review, literature introduction, etc.  

    Web of Science

    researchmap

  • Direct involvement of protein myristoylation in myristoylated alanine-rich C kinase substrate (MARCKS)-calmodulin interaction

    M Matsubara, K Titani, H Taniguchi, N Hayashi

    JOURNAL OF BIOLOGICAL CHEMISTRY   278 ( 49 )   48898 - 48902   2003.12

     More details

  • Regulation of endothelial nitric oxide synthase by protein kinase C

    M Matsubara, N Hayashi, T Jing, K Titani

    JOURNAL OF BIOCHEMISTRY   133 ( 6 )   773 - 781   2003.6

     More details

  • Nef of HIV-1 interacts directly with calcium-bound calmodulin

    N Hayashi, M Matsubara, Y Jinbo, K Titani, Y Izumi, N Matsushima

    PROTEIN SCIENCE   11 ( 3 )   529 - 537   2002.3

     More details

  • Multifunctional posttranslational modification: Na- myristoylation of proteins regulates protein-protein/protein-lipid interactions and signaling pathway systems in the brain.

    In メRecent research developments in biophysics & biochemistryモ Research Signpost   2, 33-45.   2002

     More details

  • 立体構造の描写と構造予測

    「基礎生化学実験法」(大島泰郎 編)日本生化学会   p.98-118.   2001

     More details

  • Positive charge intrinsic to Arg(37)-Arg(38) is critical for dopamine inhibition of the catalytic activity of human tyrosine hydroxylase type 1

    A Nakashima, N Hayashi, K Mori, YS Kaneko, T Nagatsu, A Ota

    FEBS LETTERS   465 ( 1 )   59 - 63   2000.1

     More details

  • Structural Changes of Calcium-bound and Calcium free Calmodulin Interacted with Trifluoperazine as Revelaed by Small-Angle X-Ray Scattering in Solution

    Biochem. J   347,211-215.   2000

     More details

  • The binding of Myristoylated N-terminal nonapeptide from neuron-specific protein CAP-23/NAP-22 to calmodulin induces a `relaxed` globular structure different from the calmodulin - non-myristoylated peptide complex

    Protein Science   9, 1905-1913.   2000

     More details

  • N末端ミリスチル化依存的NAP22-カルモジュリン間相互作用

    林宣宏, 伊藤隆, 高崎昭彦, 松原守, 柴田武彦, 谷口寿章, 千谷晃一

    日本分子生物学会年会プログラム・講演要旨集   22nd   1999

  • Identification of the calmodulin-binding domain of neuron-specific protein kinase C substrate protein CAP-22/NAP-22(jointly worked)

    J. Biol. Chem.   274 ( 17 )   11848 - 11853   1999

  • Changes in the specificity of antibodies against steroid antigens by introduction of mutations into complementarity-determining regions of the V-H domain

    Y Iba, N Hayashi, J Sawada, K Titani, Y Kurosawa

    PROTEIN ENGINEERING   11 ( 5 )   361 - 370   1998.5

     More details

    Language:English  

    Web of Science

    researchmap

  • Involvement of myristoylation in calmodulin NAP-22 interaction.

    N Hayashi, A Takasaki, M Matsubara, E Yamauchi, K Titani, H Taniguchi

    FASEB JOURNAL   12 ( 8 )   A1432 - A1432   1998.4

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • A liquid chromatography electrospray mass spectrometric study on the post-transcriptional modification of tRNA

    H Taniguchi, N Hayashi

    NUCLEIC ACIDS RESEARCH   26 ( 6 )   1481 - 1486   1998.3

     More details

  • An expression system of rat calmodulin using T7 phage promoter in Escherichia coli

    N Hayashi, M Matsubara, A Takasaki, K Titani, H Taniguchi

    PROTEIN EXPRESSION AND PURIFICATION   12 ( 1 )   25 - 28   1998.2

     More details

  • MARCKS, a major protein kinase C substrate, assumes non-helical conformations both in solution and in complex with Ca2+-calmodulin

    M Matsubara, E Yamauchi, N Hayashi, H Taniguchi

    FEBS LETTERS   421 ( 3 )   203 - 207   1998.1

  • Structures of MARCKS in solution and in complex with calmodulin.

    松原守, 山内英美子, 林宣宏, 千谷晃一, 谷口寿章

    タンパク質構造討論会講演要旨集   49th   1998

  • 蛋白質構造研究の新しい動向(共著)

    臨床病理   46   450 - 455   1998

     More details

  • Direct involvement of myristoylation in calmodulin-brain specific protein interaction(jointly worked)

    Protein Science   7(Supple.1)   157   1998

     More details

  • Recent Trends in Protein Structural Studies(jointly worked)

    46   450 - 455   1998

     More details

  • Structural analysis on the calmodulin binding domains of NO synthase

    MATSUBARA M., HAYASHI N., TITANI K., TANIGUCHI H.

    Biophysics   37   S168   1997.10

     More details

    Language:Japanese   Publisher:The Biophysical Society of Japan General Incorporated Association  

    CiNii Books

    researchmap

  • Circular dichroism and H-1 NMR studies on the structures of peptides derived from the calmodulin-binding domains of inducible and endothelial nitric-oxide synthase in solution and in complex with calmodulin - Nascent alpha-helical structures are stabilized by calmodulin both in the presence and absence of Ca2+

    M Matsubara, N Hayashi, K Titani, H Taniguchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 37 )   23050 - 23056   1997.9

     More details

  • Circular dichroism and H-1 nuclear magnetic resonance studies on the solution and membrane structures of GAP-43 calmodulin-binding domain

    N Hayashi, M Matsubara, K Titani, H Taniguchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 12 )   7639 - 7645   1997.3

     More details

  • マクロファージ型及び血管内皮細胞型一酸化窒素合成酵素のカルモジュリン結合ドメインの水溶液中とカルモジュリン複合体の構造

    松原守, 林宣宏, 千谷晃一, 谷口寿章

    日本蛋白工学会年会プログラム・要旨集   9th   1997

  • Myristoylated alanine-rich C kinase substrate(MARCKS)の構造と機能におけるミリスチル化の役割

    松原守, 林宣宏, 山内英美子, 千谷晃一, 谷口寿章

    日本蛋白工学会年会プログラム・要旨集   9th   1997

  • Complete Amino Acid Sequence of Bitiscetin, a Novel von Willebrand Factor Modulator Protein, Purified from Snake Venome of Bitis Arietans(jointly worked)

    Res. Commun. Cell. Mol. Biol.   1   271 - 284   1997

     More details

  • NMR studies of the effects of the 5'-phosphate group on conformational properties of 5-methylaminomethyluridine found in the first position of the anticodon of Escherichia coli tRNA(4)(Arg)

    K Sakamoto, G Kawai, S Watanabe, T Niimi, N Hayashi, Y Muto, K Watanabe, T Satoh, M Sekine, S Yokoyama

    BIOCHEMISTRY   35 ( 21 )   6533 - 6538   1996.5

     More details

  • Interaction between calmodulin combination domain of NO synthetase and phospholipid.

    松原守, 林宣宏, 千谷晃一, 谷口寿章

    日本分子生物学会年会プログラム・講演要旨集   19th   1996

  • The interaction of GAP-43 containing BAA motif with membrane phospholipids.

    林宣宏, 松原守, 千谷晃一, 谷口寿章

    日本蛋白工学会年会プログラム・要旨集   8th   1996

  • Expression, purification and characterization of recombinant C. Elegans UNC-18

    Hisamitsu Ogawa, Nobuhiro Hayashi, Isao Hori, Tatsuhiko Kobayashi, Ryuji Hosono

    Neurochemistry International   29 ( 5 )   553 - 563   1996

     More details

    Language:English   Publisher:Elsevier Ltd  

    DOI: 10.1016/0197-0186(95)00012-7

    Scopus

    PubMed

    researchmap

  • Structural studies of tRNAs by capillary high performance liquid chromatography/electrospray mass spectrometry(jointly worked)

    Nucleic Acids Symp. Ser.   34   153 - 154   1995

     More details

  • INTERACTION OF GAP-43 WITH MEMBRANE PHOSPHOLIPIDS - INVOLVEMENT OF PROTEIN-KINASE-C PHOSPHORYLATION DOMAIN IN THE INTERACTION

    N HAYASHI, K TITANI, H TANIGUCHI

    JOURNAL OF NEUROCHEMISTRY   65 ( Suppl )   S82 - S82   1995

     More details

    Language:English   Publishing type:Research paper, summary (international conference)  

    Web of Science

    researchmap

  • HIGHER-ORDER STRUCTURE OF BOVINE MITOCHONDRIAL TRNA(SER)UGA - CHEMICAL MODIFICATION AND COMPUTER MODELING

    Y WATANABE, G KAWAI, T YOKOGAWA, N HAYASHI, Y KUMAZAWA, T UEDA, K NISHIKAWA, HIRAO, I, K MIURA, K WATANABE

    NUCLEIC ACIDS RESEARCH   22 ( 24 )   5378 - 5384   1994.12

     More details

  • A NOVEL MODIFIED NUCLEOSIDE FOUND AT THE FIRST POSITION OF THE ANTICODON OF METHIONINE TRANSFER-RNA FROM BOVINE LIVER-MITOCHONDRIA

    J MORIYA, T YOKOGAWA, K WAKITA, T UEDA, K NISHIKAWA, PF CRAIN, T HASHIZUME, SC POMERANTZ, JA MCCLOSKEY, G KAWAI, N HAYASHI, S YOKOYAMA, K WATANABE

    BIOCHEMISTRY   33 ( 8 )   2234 - 2239   1994.3

     More details

  • UNUSUAL ANTICODON LOOP STRUCTURE FOUND IN ESCHERICHIA-COLI LYSINE TRANSFER-RNA

    K WATANABE, N HAYASHI, A OYAMA, K NISHIKAWA, T UEDA, K MIURA

    NUCLEIC ACIDS RESEARCH   22 ( 1 )   79 - 87   1994.1

     More details

  • RECOGNITION OF THE ANTICODON LOOP OF TRNA(1)(ILE) BY ISOLEUCYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI

    T NIIMI, O NUREKI, T YOKOGAWA, N HAYASHI, K NISHIKAWA, K WATANABE, S YOKOYAMA

    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   13 ( 6-7 )   1231 - 1237   1994

     More details

    Language:English  

    Web of Science

    researchmap

  • SITE-SELECTIVE HYDROLYSIS OF TRANSFER-RNA BY LANTHANIDE METAL-COMPLEXES

    N HAYASHI, N TAKEDA, T SHIIBA, M YASHIRO, K WATANABE, M KOMIYAMA

    INORGANIC CHEMISTRY   32 ( 26 )   5899 - 5900   1993.12

     More details

    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1021/ic00078a002

    Web of Science

    researchmap

  • A N-15-H-1 NUCLEAR-MAGNETIC-RESONANCE STUDY ON THE INTERACTION BETWEEN ISOLEUCINE TRANSFER-RNA AND ISOLEUCYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI

    T NIIMI, G KAWAI, M TAKAYANAGI, T NOGUCHI, N HAYASHI, T KOHNO, Y MUTO, K WATANABE, T MIYAZAWA, S YOKOYAMA

    BIOCHIMIE   75 ( 12 )   1109 - 1115   1993

     More details

    Language:English  

    Web of Science

    researchmap

  • "Tertiary structures of mitochondrial tRNAs having characteristic secondary structures" (jointly worked)

    ┣DBNucleic Acids Symp. Ser(/)-┫DB.   28   195 - 196   1993

     More details

  • "Tertiary structural analysis of ┣DBE. COLI(/)-┫DB lysine tRNA" (jointly worked)

    ┣DBNucleic Acids Symp. Ser(/)-┫DB.   28   209 - 210   1993

     More details

  • HYDROLYSIS OF OLIGODNAS BY LANTHANIDE METAL(III) CHLORIDE

    M KOMIYAMA, Y MATSUMOTO, N HAYASHI, K MATSUMURA, N TAKEDA, K WATANABE

    POLYMER JOURNAL   25 ( 11 )   1211 - 1214   1993

     More details

  • "15N- and 13C-Labeling of Escherichia coli tRNAs toward the NMR analysis"(jointly worked)

    ┣DBNucleic Acids Symp. Ser. (/)-┫DB   27   131 - 132   1992

     More details

▼display all

Awards

  • Journal of Biochemistry論文賞 Mamoru Matsubara , Nobuhiro Hayashi, Tao Jing, and Koiti Titani "Regulation of Endothelial Nitric Oxide Synthase by Protein Kinase C" J.Biochem., 133, 773-781. (2003)

    2004  

     More details

Research Projects

  • ミリストイル化蛋白質による細胞内シグナリング制御の分子機構

    2001 - 2011

      More details

    Grant type:Competitive

    researchmap

  • 脳内PKC主要基質蛋白質の構造と機能に関する研究

      More details

    Grant type:Competitive

    researchmap

  • Study on Structure and Function of Brian Major Substrate of PKC

      More details

    Grant type:Competitive

    researchmap

  • 蛋白質の構造と機能のNMRによる研究

      More details

    Grant type:Competitive

    researchmap

  • Study on Structure and Function of Protein by NMR

      More details

    Grant type:Competitive

    researchmap