2026/04/28 更新

写真a

モリ　トシアキ
森　俊明
MORI TOSHIAKI
所属
生命理工学院 准教授
職名
准教授
外部リンク

学位

  • 工学博士 （ 同志社大学 ）

研究分野

  • ライフサイエンス / 機能生物化学

  • ナノテク・材料 / 生物分子化学

  • ナノテク・材料 / 高分子化学

学歴

  • 同志社大学   工学研究科   工業化学

    - 1994年

    　 詳細を見る

    国名： 日本国

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  • 同志社大学

    - 1994年

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  • 同志社大学   工学部   工業化学

    - 1989年

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    国名： 日本国

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経歴

  • :コロラド州立大学 化学科 客員研究員

    2001年 - 2002年

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  • :Colorado State University Department of Chemistry Visiting Research Associate

    2001年 - 2002年

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  • :東京工業大学 生命理工学部 助手

    1994年 - 2000年

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  • :Tokyo Institute of Technology Fuculty of Bioscience and Bioengineering Assistant Professor

    1994年 - 2000年

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所属学協会

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委員歴

  • 日本化学会   関東支部代議員  

    2003年 - 2005年   

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    団体区分：学協会

    日本化学会

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書籍等出版物

  • 水晶発振子による糖鎖関連酵素反応のリアルタイムモニタリング

    糖鎖科学の新展開（エヌ・ティ・エス）  2005年 

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  • 超臨界流体中での分子認識と酵素反応

    超臨界流体の最新応用技術（エヌ・ティ・エス）  2004年 

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  • 脂質修飾酵素

    図解 バイオ活用技術のすべて（工業調査会）  2004年 

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  • 超臨界流体中での酵素反応

    図解バイオ活用技術のすべて（工業調査会）  2004年 

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  • Direct Monitoring of Glucan Hydrolysis by Glucoamylase on a Quartz-Crystal Microbalance

    Biomolecular Chemistry  2003年 

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  • Direct Monitoring of Glucan Hydrolysis by Glucoamylase on a Quartz-Crystal Microbalance

    Biomolecular Chemistry  2003年 

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  • ４．酵素は超臨界流体中でも使える

    グリーンバイオテクノロジー、講談社サイエンティフィック  2002年 

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  • ３節 超臨界流体中での酵素触媒反応

    超臨界流体のすべて、テクノシステム  2002年 

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  • Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media

    Methods in Biotechnology, vol. 15: Enzymes in Non-aqueous Solvents: Methods and protocols, ed. by E. N. Halling and H. L. Holland, chapter 9, Humana Press, Inc. Totowa, NJ  2001年 

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  • Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media

    Methods in Biotechnology, vol. 15: Enzymes in Non-aqueous Solvents: Methods and protocols, ed. by E. N. Halling and H. L. Holland, chapter 9, Humana Press, Inc. Totowa, NJ  2001年 

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  • 3.3 超臨界流体中での酵素反応

    超臨界流体, 佐古 猛編、アグネ承風社  2001年 

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  • Design of Two Dimensional Glycolipid Membranes and their Functions

    Precision Polymers and Nano-Organized Systems, eds by T. Kunitake, S. Nakahama, S. Takahashi, and N. Toshima, Kodansha Sci.  2000年 

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  • Design of Two Dimensional Glycolipid Membranes and their Functions

    Precision Polymers and Nano-Organized Systems, eds by T. Kunitake, S. Nakahama, S. Takahashi, and N. Toshima, Kodansha Sci.  2000年 

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  • ハイテクうらばなし 糖鎖と生命 第６回 脂質を用いて酵素反応を逆手にとる

    日刊工業新聞  1999年 

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  • Preparation of a lipid-coated enzyme and activity for reverse hydrolysis reactions in homogeneous organic media

    Enzymes in non-aqueous Media, ed. by P. Halling, Humana Press, Inc.  1999年 

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  • Preparation of a lipid-coated enzyme and activity for reverse hydrolysis reactions in homogeneous organic media

    Enzymes in non-aqueous Media, ed. by P. Halling, Humana Press, Inc.  1999年 

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MISC

  • Enzyme Reactions on a 27 MHz Quartz Crystal Microbalance

    Yoshio Okahata, Toshiaki Mori Hiroyuki Furusawa, Takanori Nihira

    Springer Series on Chemical Sensors and Biosencers   5   341 - 369   2007年

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  • Enzyme Reactions on a 27 MHz Quartz Crystal Microbalance

    Yoshio Okahata, Toshiaki Mori Hiroyuki Furusawa, Takanori Nihira

    Springer Series on Chemical Sensors and Biosencers   5   341 - 369   2007年

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  • Direct Monitoring of Both Phosphorolysis and Elongation of Amylopectin Catalyzed by Phosphorylase on a 27-MHz Quartz-Crystal Microbalance

    Akiko Murakawa, Toshiaki Mori, Yoshio Okahata

    Chem. Lett.,   36 ( 2 )   312 - 313   2007年

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  • Direct Monitoring of Both Phosphorolysis and Elongation of Amylopectin Catalyzed by Phosphorylase on a 27-MHz Quartz-Crystal Microbalance

    Akiko Murakawa, Toshiaki Mori, Yoshio Okahata

    Chem. Lett.,   36 ( 2 )   312 - 313   2007年

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  • Polymerizations of tetrafluoroethylene in homogeneous supercritical fluoroform and in detergent-free heterogeneous emulsion of supercritical fluoroform/water

    Toshiaki Mori, Yuri Tsuchiya, Yoshio Okahata

    Macromolecules   39 ( 2 )   604 - 608   2006年1月

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    記述言語：英語  

    Homogeneous polymerizations of tetrafluoroethylene (TFE) were executed in supercritical fluoroform (scCHF3), and the resulting poly(tetrafluoroethylene) (PTFE) was uniformly dispersed and twined fibers (ca. 100 nm in width and 10 μm in length). The number-average molecular weight and the conversion of PTFE can be controlled by pressures of scCHF3 from Mn = 104 to 106 and 60-80%, respectively. Heterogeneous detergent-free or stabilizer-free emulsion polymerization could be performed in the narrow condition of the scCHF3/water (5:3 in v/v) system at 17 MPa of the pressure with the 20-40% conversion, and the uniform spherical submicron particles (ca. 200 nm) of PTFE were obtained, in comparison with twined fibers from the homogeneous polymerization. © 2006 American Chemical Society.

    DOI： 10.1021/ma051930b

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  • Polymerizations of tetrafluoroethylene in homogeneous supercritical fluoroform and in detergent-free heterogeneous emulsion of supercritical fluoroform/water

    T Mori, Y Tsuchiya, Y Okahata

    MACROMOLECULES   39 ( 2 )   604 - 608   2006年1月

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    記述言語：英語   出版者・発行元：AMER CHEMICAL SOC  

    Homogeneous polymerizations of tetrafluoroethylene (TFE) were executed in supercritical fluoroform (scCHF(3)) and the resulting poly (tetrafluoroethylene) (PTFE) was uniformly dispersed and twined fibers (ca. 100 nm in width and 10 mu m in length). The number-average molecular weight and the conversion of PTFE can be controlled by pressures of scCHF(3) from M-n = 10(4) to 10(6) and 60-80%, respectively. Heterogeneous detergent-free or stabilizer-free emulsion polymerization could be performed in the narrow condition of the scCHF(3)/water (5:3 in v/v) system at 17 MPa of the pressure with the 20-40% conversion, and the uniform spherical submicron particles (ca. 200 nm) of PTFE were obtained, in comparison with twined fibers from the homogeneous polymerization.

    DOI： 10.1021/ma051930b

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  • Preparation of Hyaluronan Synthase 2 by Baculovirus-Infected Insect Cells Expression System

    Akiko Murakawa, Naoki Itano, Koji Kimata Reiji Kannagi, Toshiaki Mori, Yoshio Okahata

    Extracellular Glycomatrix in Health and Disease   1   125   2006年

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  • Direct Monitoring of Carbohydrate Elongations by Chondroitin Polymerase on a 27-MHz Quartz-Crystal Microbalance

    Toshiaki Mori, Ayaka Fujishima Nobuo Sugiura, Koji Kimata, Yoshio Okahata

    Extracellular Glycomatrix in Health and Disease   1   127   2006年

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  • Direct Monitoring of Carbohydrate Elongations by Chondroitin Polymerase on a 27-MHz Quartz-Crystal Microbalance

    Toshiaki Mori, Ayaka Fujishima Nobuo Sugiura, Koji Kimata, Yoshio Okahata

    Extracellular Glycomatrix in Health and Disease   1   127   2006年

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  • Preparation of Hyaluronan Synthase 2 by Baculovirus-Infected Insect Cells Expression System

    Akiko Murakawa, Naoki Itano, Koji Kimata Reiji Kannagi, Toshiaki Mori, Yoshio Okahata

    Extracellular Glycomatrix in Health and Disease   1   125   2006年

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  • Kinetic studies of site-directed mutational isomalto-dextranase-catalyzed hydrolytic reactions on a 27 MHz quartz-crystal microbalance

    T Nihira, M Mizuno, T Tonozuka, Y Sakano, T Mori, Y Okahata

    BIOCHEMISTRY   44 ( 27 )   9456 - 9461   2005年7月

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    記述言語：英語   出版者・発行元：AMER CHEMICAL SOC  

    A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K-d) and the catalytic process (k(cat)), separately, by using, a dextran-immobilized QCM in buffer solution. D266N, D198N, and D313N mutants, which are predicted as critical residues of the isomalto-dextranase hydrolytic activity, dramatically decreased the apparent enzyme activity. The D266N mutant, however, did not change the substrate binding ability (K-d), and the D198N and D313N mutants largely increased K-d values due to the increase of k(off) and/or the decrease of k(on) values, as well as the negatively small k(cat) values. From these results, we estimate the reaction mechanism, in which Asp266 acts as only a general acid in the catalytic process, Asp198 acts as both nucleophile in the catalytic process and binding the substrate, and Asp313 acts as only the substrate binding.

    DOI： 10.1021/bi050079q

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  • Gravimetric analyses of enzymatic glucan hydrolysis and phosphorolysis on a 27 MHz quartz-crystal microbalance

    T Mori, Y Okahata

    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY   17 ( 94 )   71 - 83   2005年3月

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    記述言語：英語   掲載種別：書評論文，書評，文献紹介等   出版者・発行元：GAKUSHIN PUBL CO  

    Catalytic cleavage reactions by glucoamylase or phosphorylase b were monitored directly on an amylopectin-immobilized 27 MHz quartz-crystal microbalance (QCM). As a first example, we could follow kinetically the enzyme binding to the substrate (binding (k(on)) and dissociation rate constants (k(off)) and intramolecular hydrolysis rate constant (k(cat)) of glucan hydrolysis by glucoamylase by detecting directly the formation and decomposition of the enzyme-substrate (ES) complex as mass changes. Secondly, glucan phosphorolysis by phosphorylase b was directly observed by two methods through reactions on a QCM. All kinetic parameters for the enzyme binding to the substrate k(on) and k(off), and dissociation constant, K-d, the AMP binding to the enzyme as activator (K-AMP), and the catalytic rate constant (k(cat)) were obtained from curve fittings of time-courses of frequency (mass) changes. The obtained kinetic parameters were compared with those from Michaelis-Menten kinetics in the bulk solution.

    DOI： 10.4052/tigg.17.71

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  • 酵素反応を重さで測る

    岡畑恵雄, 森俊明, 仁平高則

    現代化学   ( 414 )   16 - 20   2005年

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    記述言語：日本語   出版者・発行元：東京化学同人  

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  • 超臨界流体中での酵素触媒反応

    森俊明, 岡畑恵雄

    触媒   47 ( 8 )   594 - 599   2005年

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  • Enzymatic Reactions in supercritical Fluids

    Toshiaki MORI, Yoshio OKAHATA

    Catalysts & Catalysis   47 ( 8 )   594 - 599   2005年

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  • Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance

    H Nishino, T Nihira, T Mori, Y Okahata

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   126 ( 8 )   2264 - 2265   2004年3月

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    記述言語：英語   出版者・発行元：AMER CHEMICAL SOC  

    DOI： 10.1021/ja0361805

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  • Enzymatic Preparation of biotynylated N-Glycans by endoglycosidases

    T. Mori, Y. Sekine, K. Yamamoto, Y. Okahata

    Chem. Commun.   2692 - 2693   2004年

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  • Kinetic Studies of Amylopectin Cleavage by Glycogen Phosphorylase b on a 27-MHz Quartz-Crystal Microbalance

    H. Nishino, A. Murakawa, T. Mori, Y. Okahata

    J. Am. Chem. Soc.   126 ( 45 )   14752 - 14757   2004年

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  • Enzymatic Preparation of biotynylated N-Glycans by endoglycosidases

    T. Mori, Y. Sekine, K. Yamamoto, Y. Okahata

    Chem. Commun.   2692 - 2693   2004年

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  • 糖鎖の伸長・分解に関わる酵素反応を重さで測る

    森俊明, 岡畑恵雄

    バイオインダストリー   21 ( 5 )   58 - 64   2004年

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    記述言語：日本語   出版者・発行元：シーエムシー出版  

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  • 糖鎖の関わる酵素反応を重さで測る

    森俊明

    高分子   53   877 - 878   2004年

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  • Kinetic Studies of Amylopectin Cleavage by Glycogen Phosphorylase b on a 27-MHz Quartz-Crystal Microbalance

    H. Nishino, A. Murakawa, T. Mori, Y. Okahata

    J. Am. Chem. Soc.   126 ( 45 )   14752 - 14757   2004年

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  • 超臨界流体中で酵素反応を制御する

    森俊明

    化学と教育   51 ( 7 )   420 - 421   2003年

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    記述言語：日本語   出版者・発行元：公益社団法人 日本化学会  

    DOI： 10.20665/kakyoshi.51.7_420

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  • 水晶発振子上でみる糖質関連酵素

    森俊明, 岡畑恵雄

    酵素工学ニュース   ( 50 )   26 - 33   2003年

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  • 超臨界流体による酵素反応の制御

    森俊明, 岡畑恵雄

    化学工業   54 ( 2 )   93 - 97   2003年

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  • Enzymatic silicone oligomerization catalyzed by a lipid-coated lipase

    H Nishino, T Mori, Y Okahata

    CHEMICAL COMMUNICATIONS   ( 22 )   2684 - 2685   2002年11月

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    記述言語：英語   出版者・発行元：ROYAL SOC CHEMISTRY  

    A lipid (1)-coated lipase can catalyze the oligomerization of diethoxydimethylsilane (DEDMS) in isooctane containing 2wt% water, where the polymerization occurs at the OH group of the coating lipid (1) in the enzyme cavity.

    DOI： 10.1039/b206067a

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  • Reversible control of enzymatic transglycosylations in supercritical fluoroform using a lipid-coated β-D-galactosidase

    Toshiaki Mori, Ming Li, Atsushi Kobayashi, Yoshio Okahata

    Journal of the American Chemical Society   124 ( 7 )   1188 - 1189   2002年2月

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    記述言語：英語  

    The transgalactosylation rate catalyzed by the lipid-coated β-D-galactosidase in supercritical fluoroform (scCHF3) can be reversibly controlled by changing temperature or pressure (reflecting polarity changes) without damaging enzymes. Copyright © 2002 American Chemical Society.

    DOI： 10.1021/ja0169522

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  • Reversible control of enzymatic transglycosylations in supercritical fluoroform using a lipid-coated beta-D-galactosidase

    T Mori, M Li, A Kobayashi, Y Okahata

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   124 ( 7 )   1188 - 1189   2002年2月

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    記述言語：英語   出版者・発行元：AMER CHEMICAL SOC  

    DOI： 10.1021/ja0169522

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  • 超臨界流体中での酵素反応の制御

    森俊明, 岡畑恵雄

    バイオインダストリー、シーエムシー出版   19 ( 12 )   14 - 21   2002年

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  • ハイテクうらばなし 広がる舞台テーラーメードバイオ６超臨界流体

    森俊明, 岡畑恵雄

    日刊工業新聞   2002年6月18日付   2002年

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  • Contrl of Enzymes Reactions in Supercritical Fluids

    19 ( 12 )   14 - 21   2002年

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  • A Lipid-coated Lipase as an Efficient Hydrolytic Catalyst in the Two-phase Aqueous-Organic System

    T. Mori, S. Kishimoto, K. Ijiro, A. Kobayashi, Y. Okahata

    Biotech. & Bioeng.   76 ( 2 )   157 - 163   2001年

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  • A Lipid-coated Lipase as an Efficient Hydrolytic Catalyst in the Two-phase Aqueous-Organic System

    T. Mori, S. Kishimoto, K. Ijiro, A. Kobayashi, Y. Okahata

    Biotech. & Bioeng.   76 ( 2 )   157 - 163   2001年

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  • Reversible activity changes of a lipid-coated lipase for enantioselective esterification in supercritical fluoroform

    T. Mori, M. Funasaki, A. Kobayashi, Y. Okahata

    Chem. Commun.   ( 18 )   1832 - 1833   2001年

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  • 超臨界流体中での酵素反応の制御 －超臨界フルオロフォルム中での脂質修飾リパーゼを用いた不斉選択的エステル化反応－

    森俊明, 舟崎真理子, 小林厚志, 岡畑恵雄

    高分子論文集、超臨界特集号   2001年

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  • Reversible activity changes of a lipid-coated lipase for enantioselective esterification in supercritical fluoroform

    T. Mori, M. Funasaki, A. Kobayashi, Y. Okahata

    Chem. Commun.   ( 18 )   1832 - 1833   2001年

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  • 超臨界フルオロフォルム中での温度や圧力変化を利用した溶媒和による反応の制御 －脂質修飾リパーゼを用いた不斉選択的エステル化反応を例として－

    岡畑恵雄, 森俊

    JASCOレポート   2001年

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  • Nucleobase Molecular Recognition in Supercritical Carbon Dioxide by using a Highly Sensitive 27 MHz Quart.-Crystal Microbalance

    T. Mori, M. Naito, Y. Irimoto, Y. Okahata

    Chem. Commun.   ( 1 )   45 - 46   2000年

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  • Nucleobase Molecular Recognition in Supercritical Carbon Dioxide by using a Highly Sensitive 27 MHz Quart.-Crystal Microbalance

    T. Mori, M. Naito, Y. Irimoto, Y. Okahata

    Chem. Commun.   ( 1 )   45 - 46   2000年

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  • A Lipid-coated Lipase as an Effective Hydrolytic catalyst in the Two-phase Aqueous-Organic System

    Toshiaki Mori Shintaro Kishimoto Kuniharu Ijiro Atsushi Kobayashi, Yoshio Okahata

    Biotech. Bioeng.   54   350 - 358   2000年

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  • 超臨界流体中での酵素触媒反応

    森俊明, 岡畑恵雄

    化学と生物   38 ( 1 )   27 - 29   2000年

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    記述言語：日本語   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry  

    DOI： 10.1271/kagakutoseibutsu1962.38.27

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    その他リンク： https://jlc.jst.go.jp/DN/JALC/00086850384?from=CiNii

  • A Lipid-coated Lipase as an Effective Hydrolytic catalyst in the Two-phase Aqueous-Organic System

    Toshiaki Mori Shintaro Kishimoto Kuniharu Ijiro Atsushi Kobayashi, Yoshio Okahata

    Biotech. Bioeng.   54   350 - 358   2000年

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  • 生体触媒を使いこなす(5)-超臨界流体中での生体触媒反応

    森俊明, 岡畑恵雄

    現代化学   353   60 - 66   2000年

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  • 超臨界流体を反応媒体とした酵素触媒反応

    森俊明, 岡畑恵雄

    JASCO・レポート   4   36 - 40   2000年

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  • Cast films of lipid-coated enzymes as selective sensors for disaccharides

    T Mori, T Motonaga, Y Okahata

    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS   146 ( 1-3 )   387 - 395   1999年1月

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    記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

    A stable enzyme film was prepared on a platinum electrode by casting organic solutions of a lipid-coated enzyme. The glucose oxidase (GOD)-immobilized electrode acted as a glucose sensor with a high sensitivity and a short response time. When the lipid-coated GOD was combined with a lipid-coated glycosidase on the electrode, the film showed a high selectivity for various disaccharides. For example, combining beta-galactosidase with GOD, the electrode responded only to lactose (Gal beta 1 --> 4Glc), having beta-galactoside at the non-reducing side of the disaccharide, but not to maltose (Glc alpha 1 --> 4Glc) and cellobiose (GlcB1-t4Glc) in the aqueous solution. Similarly, an enzyme electrode sensitive only for maltose (Glc alpha 1 --> 4Glc), cellobiose (Glc beta 1 --> 4Glc), and sucrose (Glc alpha 1 --> 2Fru) could be prepared by using a mixed cast film of two lipid-coated enzymes, GOD and alpha-glucosidase, GOD and beta-glucosidase, and GOD and invertase, respectively. This technique will become a new process not only for preparing sensor membranes but also for assembling water-soluble proteins on biological electrical components. (C) 1999 Elsevier Science B.V. All rights reserved.

    DOI： 10.1016/S0927-7757(98)00808-5

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  • Enzymatic Syntheses of Glycolipids catalyzed by a Lipid-coated Glycoside Hydrolase in the Organic-Aqueous Two Phase System

    T. Mori, S. Fukusho, J. Kojima, Y. Okahata

    Polym. J.   31 ( 11-2 )   1105 - 1108   1999年

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  • Enzymatic Syntheses of Glycolipids catalyzed by a Lipid-coated Glycoside Hydrolase in the Organic-Aqueous Two Phase System

    T. Mori, S. Fukusho, J. Kojima, Y. Okahata

    Polym. J.   31 ( 11-2 )   1105 - 1108   1999年

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  • 糖鎖ライブラリー構築のための固相グリコシル化反応

    森俊明

    化学と工業   52   1182   1999年

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  • 酵素を有機溶媒に溶かして配糖化反応を行う

    岡畑恵雄, 森俊

    FFIジャーナル   179   5 - 10   1999年

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  • 超臨界流体中での生体触媒反応

    森俊明, 岡畑恵雄

    化学と工業   52   1502 - 1505   1999年

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  • Transglycosylation catalyzed by a lipid-coated beta-D-galactosidase in a two-phase aqueous-organic system

    Y Okahata, T Mori

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   5 ( 1-4 )   119 - 123   1998年9月

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    記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

    A lipid-coated enzyme was prepared in which the enzyme surface is covered with a lipid monolayer and two long alkyl lipophilic tails serve to solubilize the enzyme in organic solvents. In a two-phase aqueous-organic system, a lipid-coated beta-D-galactosidase exists in the organic (2-propyl ether) phase and acts as an efficient transgalactosylation catalyst for various hydrophobic alcohols with lactose in the aqueous buffer solution. When a native beta-D-galactosidase was employed in the two-phase system, neither the transgalactosylation nor the hydrolysis reaction proceeded due to denaturation of the enzyme at the interface. (C)1998 Elsevier Science B.V. All rights reserved.

    DOI： 10.1016/S1381-1177(98)00088-5

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  • Biocatalytic esterification in supercritical carbon dioxide by using a lipid-coated lipase

    Toshiaki Mori, Atsushi Kobayashi, Yoshio Okahata

    Chemistry Letters   ( 9 )   921 - 922   1998年

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    記述言語：英語   出版者・発行元：Chemical Society of Japan  

    A lipid-coated lipase is soluble and acts as a more efficient catalyst for triglyceride syntheses in supercritical carbon dioxide (scCO2) than in conventional organic solvents, and the reactivity can be controlled reversibly by changing pressure and temperature in scCO2.

    DOI： 10.1246/cl.1998.921

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  • Effective Biocatalytic Transglycosylation in a Supercritical Fluid by using a lipid-coated Enzyme

    Toshiaki Mori, Yoshio Okahata

    Chem. Commun.   ( 20 )   2215 - 2216   1998年

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  • Biocatalytic Reactions in Organic media by using a Lipid-coated Enzyme

    Yoshio Okahata, Toshiaki Mori

    J. Synth. Org. Chem., Jpn.   56 ( 11 )   931 - 939   1998年

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  • Vertical Standing of Amphiphilic Helical Peptides at the Hexane-Water Interface

    Kiyonobu Kishihara Takatoshi Kinoshita Toshiaki Mori, Yoshio Okahata

    Chem. Lett.   ( 9 )   951 - 952   1998年

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  • Biocatalytic Esterification in Supercritical Carbon Dioxide by Using a Lipid-coated Lipase

    Toshiaki Mori, Atsushi Kobayashi, Yoshio Okahata

    Chem. Lett.   ( 9 )   921 - 922   1998年

    　詳細を見る

  • Effective Biocatalytic Transglycosylation in a Supercritical Fluid by using a lipid-coated Enzyme

    Toshiaki Mori, Yoshio Okahata

    Chem. Commun.   ( 20 )   2215 - 2216   1998年

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  • Biocatalytic Reactions in Organic media by using a Lipid-coated Enzyme

    Yoshio Okahata, Toshiaki Mori

    J. Synth. Org. Chem., Jpn.   56 ( 11 )   931 - 939   1998年

    　詳細を見る

  • Vertical Standing of Amphiphilic Helical Peptides at the Hexane-Water Interface

    Kiyonobu Kishihara Takatoshi Kinoshita Toshiaki Mori, Yoshio Okahata

    Chem. Lett.   ( 9 )   951 - 952   1998年

    　詳細を見る

  • A variety of lipid-coated glycoside hydrolases as effective glycosyl transfer catalysts in homogeneous organic solvents

    T Mori, Y Okahata

    TETRAHEDRON LETTERS   38 ( 11 )   1971 - 1974   1997年3月

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    記述言語：英語   出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD  

    Various lipid-coated glycoside hydrolases were prepared, which act as efficient catalysts for transglycosylation of alpha-D- and beta-D-mannoside, N-acetyl-beta-D-glucosaminide, beta-D-glucoside, and beta-D-galactoside to hydrophobic acceptor alcohols in dry isopropyl ether. The enzyme activity for the glycosylation depended on coating lipids as well as origin or kind of enzymes. (C) 1997 Elsevier Science Ltd.

    DOI： 10.1016/S0040-4039(97)00251-7

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  • Lipid-coated enzymes as efficient catalysts in organic media

    Y Okahata, T Mori

    TRENDS IN BIOTECHNOLOGY   15 ( 2 )   50 - 54   1997年2月

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    記述言語：英語   出版者・発行元：ELSEVIER SCI LTD  

    A new way of solubilizing hydrolytic enzymes so that they can be used in organic solvents is to coat them with lipid monolayers, Lipid-coated enzymes have been used successfully for a number of different types of reactions in organic media or organic-aqueous, two-phase systems; for example, enantioselective esterification by lipase, transphosphatidylation of water-insoluble phospholipids by phospholipase D, hydrolysis of a lipophilic substrate by a catalytic antibody and transglycosylation by beta-D-galactosidase.

    DOI： 10.1016/S0167-7799(97)84203-5

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  • A Facile Transgalactosylation catalyzed by a Lipid-Coated β-D-Galactosidase in the Water-Organic Two Phases

    Toshiaki Mori, Sanae Fujita, Yoshio Okahata

    Chem. Lett.   ( 1 )   73 - 74   1997年

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  • Transglycosylation in a two-phase aqueous-organic system with catalysis by a lipid-coated β-D-galactosidase

    Toshiaki Mori, Sanae Fujita, Yoshio Okahata

    Carbohydr. Res.   298   65 - 73   1997年

    　詳細を見る

  • A Facile Transglycosylation Catalyzed by Lipid-coated Glycoside Hydrolases

    44 ( 3 )   337 - 342   1997年

    　詳細を見る

  • Catalytic Activity of Lipid-coated Enzymes in Organic Media

    Yoshio Okahata, Toshiaki Mori

    Proc. Japan Acad.   73 ( 10 )   210 - 214   1997年

    　詳細を見る

  • A Facile Transgalactosylation catalyzed by a Lipid-Coated β-D-Galactosidase in the Water-Organic Two Phases

    Toshiaki Mori, Sanae Fujita, Yoshio Okahata

    Chem. Lett.   ( 1 )   73 - 74   1997年

    　詳細を見る

  • Transglycosylation in a two-phase aqueous-organic system with catalysis by a lipid-coated β-D-galactosidase

    Toshiaki Mori, Sanae Fujita, Yoshio Okahata

    Carbohydr. Res.   298   65 - 73   1997年

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  • 脂質修飾法を用いる糖加水分解酵素の改変 -有機溶媒中での配糖化反応-

    森俊明, 岡畑恵雄

    応用糖質科学   44 ( 3 )   337 - 342   1997年

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    記述言語：日本語   出版者・発行元：The Japanese Society of Applied Glycoscience  

    Various lipid-coated glycoside hydrolases were prepared, which were soluble in most organic solvents such as isopropyl ether, isooctane and benzene but insoluble in aqueous solution. They could act as efficient catalysts for transglycosylation of α-D-or β-D-mannoside, N-acetyl-β-D-glucosaminide, β-D-glucoside or β-D-galactoside to hydrophobic acceptor alcohols in dry isopropyl ether. When a native β-D-galactosidase was used in the aqueous solution containing acetonitrile for the same reaction (a conventional method), the yield of the transgalactosylation was low due to the predominant process of hydrolysis reaction. The enzyme activity for glycosylation depends on the coating of lipids as well as the orgin or type of enzyme. These lipid-coated glycoside hydrolases could also act as an efficient catalyst for transglycosylation in the two water-organic phases: both the hydrophobic lipid-coated enzyme and alcohols were solubilized in isopropyl ether and mixed with an aqueous solution of glycosyl donors such as lactose and cellobiose.

    DOI： 10.11541/jag1994.44.337

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    その他リンク： https://jlc.jst.go.jp/DN/JALC/10013090783?from=CiNii

  • Catalytic Activity of Lipid-coated Enzymes in Organic Media

    Yoshio Okahata, Toshiaki Mori

    Proc. Japan Acad.   73 ( 10 )   210 - 214   1997年

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  • 「有機合成化学で活躍する生体触媒」3. 有機溶媒中の均一系生体触媒反応

    森俊明, 岡畑恵雄

    化学と生物   35 ( 9 )   662 - 666   1997年

    　詳細を見る

    記述言語：日本語   出版者・発行元：学会出版センタ-  

    DOI： 10.1271/kagakutoseibutsu1962.35.662

    CiNii Books

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  • Effective Transgalactosylation catalysed by a lipid-coated β-D-galactosidase in organic solvents

    Yoshio Okahata, Toshiaki Mori

    J. Chem. Soc. , Perkin Trans 1   2861 - 2866   1996年

    　詳細を見る

  • Effective Transgalactosylation catalysed by a lipid-coated β-D-galactosidase in organic solvents

    Yoshio Okahata, Toshiaki Mori

    J. Chem. Soc. , Perkin Trans 1   2861 - 2866   1996年

    　詳細を見る

  • Organic Synthesis catalyzed by a Lipid-coated Enzyme in Organic Solvent

    33 ( 1 )   10 - 17   1995年

    　詳細を見る

  • 脂質修飾酵素の有機溶媒中での触媒作用

    森俊明, 岡畑恵雄

    表面   33 ( 1 )   10 - 17   1995年

    　詳細を見る

  • Two-Dimensional Array of Poly(methacrylic acid) Brushes on Gold Substrates. Interaction with Ferrocene-terminated Poly(oxyethylenes)

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Macromolecules   28 ( 23 )   7770 - 7774   1995年

    　詳細を見る

  • Two-Dimensional Array of Poly(methacrylic acid) Brushes on Gold Substrates. Interaction with Ferrocene-terminated Poly(oxyethylenes)

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Macromolecules   28 ( 23 )   7770 - 7774   1995年

    　詳細を見る

  • Two-Dimensional Array of Poly(methacrylic acid)Brushes on Gold Substrates. Interaction with Ferrocene-Terminated Poly(oxyethylene)s

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Macromolecules   28 ( 23 )   7770 - 7774   1995年

    　詳細を見る

  • Two-Dimensional Array of Poly(methacrylic acid)Brushes on Gold Substrates. Interaction with Ferrocene-Terminated Poly(oxyethylene)s

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Macromolecules   28 ( 23 )   7770 - 7774   1995年

    　詳細を見る

  • Chain-length Recognition of Ferrocene-terminated Poly(oxyethylenes)by Poly(methacrylic acid)-functionalized Monolayers

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Trans. Mater. Res. Soc. Jpn.   15A   607 - 610   1994年

    　詳細を見る

  • Chain-length recognition of ferrocene-terminated poly(oxyethylenes) by poly(methacrylic acid)-functionalized monolayers on gold electrodes

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Trans. Mater. Res. Soc. Jpn.   15A   607 - 610   1994年

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  • Chain-length Recognition of Ferrocene-terminated Poly(oxyethylenes)by Poly(methacrylic acid)-functionalized Monolayers

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Trans. Mater. Res. Soc. Jpn.   15A   607 - 610   1994年

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  • Chain-length recognition of ferrocene-terminated poly(oxyethylenes) by poly(methacrylic acid)-functionalized monolayers on gold electrodes

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Trans. Mater. Res. Soc. Jpn.   15A   607 - 610   1994年

    　詳細を見る

  • SPECIFIC ADSORPTION OF FERROCENE-TERMINATED POLY(OXYETHYLENE) AT SELF-ASSEMBLED MONOLAYERS OF POLY(METHACRYLIC ACID)-BASED AMPHIPHILES ON GOLD ELECTRODES

    M NIWA, T MORI, N HIGASHI

    JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS   ( 13 )   1081 - 1083   1993年7月

    　詳細を見る

    記述言語：英語   出版者・発行元：ROYAL SOC CHEMISTRY  

    Self-assembled monolayers of poly(methacrylic acid)-based amphiphiles (1n) on gold electrodes have the ability to discriminate against the chain-length of ferrocene-terminated poly(oxyethylene) (2m) on the basis of an electrochemical measurement.

    DOI： 10.1039/c39930001081

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  • CONTROL OF ADSORPTION STATE BASED ON CONFORMATIONAL CHANGE OF POLY(METHACRYLIC ACID)-FUNCTIONALIZED, DOUBLE-CHAIN MONOLAYERS ON GOLD SUBSTRATES

    M NIWA, T MORI, N HIGASHI

    MACROMOLECULES   26 ( 8 )   1936 - 1940   1993年4月

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    記述言語：英語   出版者・発行元：AMER CHEMICAL SOC  

    Polymeric amphiphiles (1n) containing poly(methacrylic acid) (PMAA) segments having different chain lengths (n = 35, 63, and 177) and two long alkyl chains whose ends are modified with disulfide groups are newly prepared, and ellipsometric and electrochemical studies of the 1n monolayer-covered gold substrates are described. The 1n are spontaneously adsorbed onto gold substrates from aqueous solutions with various pHs and form monolayer assemblies. The ellipsometric thickness of the resulting monolayers is markedly affected by the PMAA chain length (n) and/or the surrounding pH during adsorption: the thicknesses increase both with increasing n and with increasing pH. These changes in thickness are governed by the conformation of the PMAA segments since the data can be rationalized in terms of the molecular size of the PMAA segments based on their extent in the conformational state. Electrochemical redox measurements on the monolayers provide information about the lateral orientation (molecular packing) of 1n within the monolayers. The data suggest that the packing density of 1(63) in the monolayer adsorbed at pH 3.0 is much higher than that adsorbed at pH 8.2, reflecting the conformational size of the PMAA segment adopted at each pH. The immobilized monolayer on the gold substrate gives reversible changes in the ellipsometric thickness and in the electrochemical response by varying pH, though the changes are dependent upon the molecular packing density on the substrates.

    DOI： 10.1021/ma00060a021

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  • Control of Adsorption State Based on Conformational Change of Poly(methacrylic acid)-functionalized, Double-chain Monolayers on Gold Substrates

    Masazo Niwa, Toshiaki Nobuyuki Higashi

    Macromolecules   26 ( 8 )   1936 - 1940   1993年

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  • Specific adsorption of ferrocene-terminated poly(oxyethylene) at self-assembled monolayers of poly(methacrylic acid)-based amphiphiles on gold electrodes

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    Journal of the Chemical Society, Chemical Communications   ( 13 )   1081 - 1083   1993年

    　詳細を見る

    記述言語：英語  

    Self-assembled monolayers of poly(methacrylic acid)-based amphiphiles (1n) on gold electrodes have the ability to discriminate against the chain-length of ferrocene-terminated poly(oxyethylene) (2m) on the basis of an electrochemical measurement.

    DOI： 10.1039/C39930001081

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  • Specific Binding of a Lectin to Glycolipid Monolayers on Gold Electrodes

    Rep. Chem. Mat. R&D Found.   8   7 - 11   1993年

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  • Specific Adsorption of Ferrocene-terminated Poly(oxyethylene) at Self-assembled Monolayers Poly(methacrylic acid)-based Amphiphiles on Gold Electrodes

    Masazo Niwa, Toshiaki Mori, Nobuyuki Higashi

    J.Chem. Soc., Chem. Commun.   ( 13 )   1081 - 1083   1993年

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  • 糖脂質単分子膜の金電極上での自発形成とレクチンタンパクとの特異的結合

    東信行, 森俊明, 丹羽政三

    化学素材研究開発振興財団研究報告   8   7 - 11   1993年

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  • SPECIFIC BINDING OF CONCANAVALIN-A TO GLYCOLIPID MONOLAYERS ON GOLD ELECTRODES

    M NIWA, T MORI, E NISHIO, H NISHIMURA, N HIGASHI

    JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS   ( 7 )   547 - 549   1992年4月

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    記述言語：英語   出版者・発行元：ROYAL SOC CHEMISTRY  

    Formation of monolayers of disulfide-containing glycolipids by spontaneous adsorption onto gold electrodes and their specific binding properties to a lectin are described.

    DOI： 10.1039/c39920000547

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  • Two-dimensional Photopolymerization at Adsorbed Monolayers on Gold

    Masazo Niwa, Toshiaki Nobuyuki Higashi

    Journal of Material Chemistry   2   245 - 251   1992年

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  • Two-dimensional photopolymerization at adsorbed monolayers on gold

    Masazo Niwa, Toshlakl Morl, Nobuyuki Nlgashi

    Journal of Materials Chemistry   2 ( 2 )   245 - 251   1992年

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    記述言語：英語  

    Disulfide-bearing ammonium amphiphiles with different counter-anions, Cl- (1Cl) styrene-p-sulfonate (1SS), and poly(styrene-p-sulfonate) (1PSS), and a photoinitiator bearing a dithiocarbonate group (2) have been prepared. They formed monolayers on gold by spontaneous adsorption from organic solutions. Surface properties of these monolayers have been estimated by contact-angle and electrochemical measurements. Both techniques showed that there are distinct differences in surface properties among counter-anions. The surface free energy calculated from the results of water and methylene iodide contact angle decreased in the order: 1Cl &gt
    1SS &gt
    1PSS, dependent upon the hydrophobicity of the counter-anions. Reverse trends (1Cl &lt
    1SS &lt
    1PSS) were observed in the electrochemical barrier capability of the monolayers toward Fe(CN)4- 6 Photopolymerizations of 1SS monolayers have been studied by cyclic voltammetry using Fe(CN)4-6 as an electroactive species. The polymerization process could be traced on the basis of reduction of the redox peak currents. For the 1SS-2 ([2]: [1SS] = 0.5%) monolayer, polymerization proceeded smoothly upon UV irradiation, with completion of the polymerization after ca. 3 h. The pure 1SS monolayer (without 2) showed only a slight decrease in the redox peak current, indicating that effective polymerization did not occur. Binary monolayers composed of 1SS and a fluoroalkanethiol (3) could also be formed on gold from solution, and their photopolymerization behaviour has also been studied. With decreasing 1SS content in the mixture, the redox peak currents increased with UV irradiation, in contrast to the results of the single-component (1SS) monolayer, suggesting formation of a boundary void between phase-separated domains of polymerized 1SS and 3.

    DOI： 10.1039/jm9920200245

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  • A Novel Two-dimensional Photopolymerization at an Ordered Monolayer Surface on Gold

    Nobuyuki Higashi, Toshiaki Mori, Masazo Niwa

    Journal of Chemical Society,Chemical communication   ( 3 )   225 - 226   1990年

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  • A novel two-dimensional photopolymerization at an ordered monolayer surface on gold

    Nobuyuki Higashi, Toshiaki Mori, Masazo Niwa

    Journal of the Chemical Society, Chemical Communications   ( 3 )   225 - 226   1990年

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    記述言語：英語  

    A two-dimensional photopolymerization has been successfully carried out in the presence of a photoinitiator fixed near the polymerizable groups at a spontaneously adsorbed monolayer surface on gold.

    DOI： 10.1039/C39900000225

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▼全件表示

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    資金種別：競争的資金

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    2000年 - 2002年

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