Updated on 2026/03/05

写真a

 
ISHII YOSHITAKA
 
Organization
School of Life Science and Technology Professor
Title
Professor
External link

News & Topics

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Degree

  • Ph. D.

Research Interests

  • Alzheimer's

  • amyloid

  • アルツハイマー

  • アミロイド

  • Solid-state NMR

  • "Solid-state NMR"

  • NMR

Research History

  • Institute of Science Tokyo   Professor

    2024.10

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  • Tokyo Institute of Technology   School of Life Science and Technology   Professor

    2017.1 - 2024.9

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Professional Memberships

Papers

  • Analysis of solid-state NMR data facilitated by MagRO_NMRViewJ with Graph_Robot: Application for membrane protein and amyloid. Reviewed

    Naohiro Kobayashi, Yoshitaka Ishii

    Biophysical Chemistry   318   107356 - 107356   2025.3

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bpc.2024.107356

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  • E22G Aβ40 fibril structure and kinetics illuminate how Aβ40 rather than Aβ42 triggers familial Alzheimer's. Reviewed International coauthorship International journal

    Mohammad Jafar Tehrani, Isamu Matsuda, Atsushi Yamagata, Yu Kodama, Tatsuya Matsunaga, Mayuko Sato, Kiminori Toyooka, Dan McElheny, Naohiro Kobayashi, Mikako Shirouzu, Yoshitaka Ishii

    Nature communications   15 ( 1 )   7045 - 7045   2024.8

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Arctic (E22G) mutation in amyloid-β (Aβ enhances Aβ40 fibril accumulation in Alzheimer's disease (AD). Unlike sporadic AD, familial AD (FAD) patients with the mutation exhibit more Aβ40 in the plaque core. However, structural details of E22G Aβ40 fibrils remain elusive, hindering therapeutic progress. Here, we determine a distinctive W-shaped parallel β-sheet structure through co-analysis by cryo-electron microscopy (cryoEM) and solid-state nuclear magnetic resonance (SSNMR) of in-vitro-prepared E22G Aβ40 fibrils. The E22G Aβ40 fibrils displays typical amyloid features in cotton-wool plaques in the FAD, such as low thioflavin-T fluorescence and a less compact unbundled morphology. Furthermore, kinetic and MD studies reveal previously unidentified in-vitro evidence that E22G Aβ40, rather than Aβ42, may trigger Aβ misfolding in the FAD, and prompt subsequent misfolding of wild-type (WT) Aβ40/Aβ42 via cross-seeding. The results provide insight into how the Arctic mutation promotes AD via Aβ40 accumulation and cross-propagation.

    DOI： 10.1038/s41467-024-51294-w

    PubMed

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  • NMR Studies of Genomic RNA in 3′ Untranslated Region Unveil Pseudoknot Structure that Initiates Viral RNA Replication in SARS-CoV-2 Reviewed

    Takako Ohyama, Takuo Osawa, Shun-ichi Sekine, Yoshitaka Ishii

    JACS Au   2024.4

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/jacsau.3c00641

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  • Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in 1H-detected protein solid-state NMR Reviewed

    Yoshiki Shigemitsu, Yuki Miyazaki, Hibiki Terami, Daria Ostapova, Tatsuya Matsunaga, Ryo Takahashi, Takumi Inoue, Toshio Yamazaki, Yoshitaka Ishii

    Journal of Biomolecular NMR   2025.11

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10858-025-00476-9

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MISC

  • Secondary structure determination of functional domain of functional long non-coding RNA

    大山貴子, 大山貴子, 高橋葉月, 大澤拓生, CARNINCI Piero, 関根俊一, 石井佳誉, 石井佳誉

    日本分子生物学会年会プログラム・要旨集(Web)   46th   2023

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Industrial property rights

  • コロナウイルス感染症治療薬のスクリーニング方法

    大山 貴子, 石井 佳誉

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    Applicant：国立研究開発法人理化学研究所, 石井 佳誉

    Application no：特願2024-034653  Date applied：2024.3

    Announcement no：特開2024-128959  Date announced：2024.9

    J-GLOBAL

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  • エピスルフィド構造の定量方法

    北浦 健大, 大内 宗城, 石井 佳誉

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    Applicant：住友ゴム工業株式会社, 国立研究開発法人理化学研究所, 石井 佳誉

    Application no：特願2022-077598  Date applied：2022.5

    Announcement no：特開2023-166806  Date announced：2023.11

    J-GLOBAL

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  • 環状スルフィド構造の定量方法

    石井 佳誉, 大内 宗城, 北浦 健大

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    Applicant：国立大学法人東京科学大学, 石井 佳誉, 国立研究開発法人理化学研究所, 住友ゴム工業株式会社

    Application no：特願2020-188025  Date applied：2020.11

    Announcement no：特開2022-077256  Date announced：2022.5

    Patent/Registration no：特許第7704350号  Date registered：2025.6 

    J-GLOBAL

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Research Projects

  • New strategies for amorphous biopolymers and advanced materials structure analysis by ultra-high resolution solid-state NMR and automated analysis

    Grant number：22K18328  2022.6 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)  Grant-in-Aid for Challenging Research (Pioneering)

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    Grant amount：\25870000 （ Direct Cost: \19900000 、 Indirect Cost：\5970000 ）

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  • 多機能化一本鎖抗体によるアミロイドβ凝集体細胞内スワイプ療法の開発

    Grant number：22K18380  2022.6 - 2025.3

    日本学術振興会  科学研究費助成事業  挑戦的研究(開拓)

    星 美奈子, 村松 慎一, 笹原 智也, 石井 佳誉, 豊島 近, 喜井 勲

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    Grant amount：\26000000 （ Direct Cost: \20000000 、 Indirect Cost：\6000000 ）

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