Updated on 2026/04/28

写真a

 
OKOCHI MINA
 
Organization
School of Materials and Chemical Technology Professor
Title
Professor
Homepage
http://www.nubio.nagoya-u.ac.jp/proc/index.htm
External link

News & Topics

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Education

  • 日本学術振興会特別研究員（東京農工大学）

    - 1998

    　 More details

  • Tokyo University of Agriculture and Technology

    - 1998

    　 More details

  • Tokyo University of Agriculture and Technology

    - 1996

    　 More details

    Country： Japan

    researchmap

  • Tokyo University of Agriculture and Technology   Faculty of Engineering

    - 1993

    　 More details

Research History

  • 東京工業大学大学院   理工学研究科（改組により2016年から物質理工学院に所属）   教授

    2014.4

    　 More details

  • 名古屋大学大学院   工学研究科 化学生物工学専攻   准教授

    2004.10 - 2014.3

    　 More details

  • Tokyo University of Agriculture and Technology   Faculty of Engineering, Department of Biotechnology and Life Science

    1999.9 - 2004.9

    　 More details

Papers

  • Exploration and analytical techniques for membrane curvature-sensing proteins in bacteria. International journal

    Takumi Komikawa, Mina Okochi, Masayoshi Tanaka

    Journal of bacteriology   207 ( 4 )   e0048224   2025.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    The mechanism by which cells regulate protein localization is an important topic in the field of bacterial biology. In certain instances, the morphology of the biological membrane has been demonstrated to function as a spatial cue for the subcellular localization of proteins. These proteins are capable of sensing membrane curvature and are involved in a number of physiological functions such as cytokinesis and the formation of membrane-bound organelles. This review presents recent advances in the in vitro evaluation of curvature-sensing properties using artificially controlled membranes and purified proteins, as well as microscopic live cell assays. However, these evaluation methodologies often require sophisticated experiments, and the number of identified curvature sensors remains limited. Thus, we present a comprehensive exploration of recently reported curvature-sensing proteins. Subsequently, we summarize the known curvature-sensing proteins in bacteria, in conjunction with the analytical methodologies employed in this field. Finally, future prospects and further requirements in the study of curvature-sensing proteins are discussed.

    DOI： 10.1128/jb.00482-24

    PubMed

    researchmap

  • Synthesis of atom-precise supported metal clusters via solid-phase peptide synthesis. International journal

    Takane Imaoka, Nanami Antoku, Yusuke Narita, Kazuki Nishiyama, Kenji Takada, Shogo Saito, Masayoshi Tanaka, Mina Okochi, Miftakhul Huda, Makoto Tanabe, Wang-Jae Chun, Kimihisa Yamamoto

    Chemical science   15 ( 36 )   14931 - 7   2024.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    While the utility of supported metal and alloy clusters as catalytic materials is widely recognized, their precise synthesis remains a challenge. Here, we demonstrate the precise synthesis of these clusters via metallopeptides. This technique is characterized by its ability to be automated using Merrifield's solid-phase peptide synthesis (SPPS). Metallopeptides with iron and platinum complexes in their side chains have been prepared using this SPPS. These metallopeptides were successfully transformed into the corresponding supported metal clusters by heating in a hydrogen atmosphere.

    DOI： 10.1039/d4sc04400b

    PubMed

    researchmap

  • Peptide array screening with anti-GLP-1 monoclonal antibody: Discovery of cysteine-containing DPP-IV inhibitory peptides Reviewed

    Masaki Kurimoto, Naoki Yuda, Masayoshi Tanaka, Miyuki Tanaka, Mina Okochi

    Journal of Bioscience and Bioengineering   2024.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2024.07.001

    researchmap

  • Small Au Nanoparticles Synthesized by Peptide-Based Biomineralization for Catalytic Applications

    Masayoshi Tanaka, Yuka Kiriki, Nozomi Kiyohara, Mirei Hayashi, Abiral Tamang, Tomonori Nakamura, Martin Vacha, Yonghyun Choi, Jonghoon Choi, Wataru Yoshida, Kevin Critchley, Stephen D. Evans, Mina Okochi

    ACS Applied Nano Materials   2024.5

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsanm.4c00780

    researchmap

  • Enantioselective Detection of Gaseous Odorants with Peptide–Graphene Sensors Operating in Humid Environments Reviewed

    Yui Yamazaki, Tatsuru Hitomi, Chishu Homma, Tharatorn Rungreungthanapol, Masayoshi Tanaka, Kou Yamada, Hiroshi Hamasaki, Yoshiaki Sugizaki, Atsunobu Isobayashi, Hideyuki Tomizawa, Mina Okochi, Yuhei Hayamizu

    ACS Applied Materials & Interfaces   2024.4

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acsami.4c01177

    researchmap

  • Osmotic stress induces the formation of migrasome-like vesicles. International journal

    Koki Yoshikawa, Shogo Saito, Tetsuya Kadonosono, Masayoshi Tanaka, Mina Okochi

    FEBS letters   2024.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Migrasomes are extracellular vesicles that form on the retraction fibers of migrating cells. In this study, we report the formation of migrasome-like vesicles enriched in tetraspanin 4 and containing cytoplasmic components in response to hypoosmotic stress. When migrating cells were subjected to hypoosmotic stress, vesicles with a size distribution of 0.5 to 2 μm formed on the retraction fibers, and vanished in a few minutes. The vesicles are rich in cholesterol, and their number was reduced when cells were pretreated with lipoprotein-deficient serum. The formation of migrasome-like vesicles upon hypoosmotic stress may provide biophysical cues regarding the cellular response to this external stimulus in cells and tissues.

    DOI： 10.1002/1873-3468.14816

    PubMed

    researchmap

  • Screening of novel DPP-IV inhibitory peptides derived from bovine milk proteins using a peptide array platform Reviewed

    Sayuri Arai, Masaki Kurimoto, Hajime Nakada, Masayoshi Tanaka, Hiroshi Ochi, Miyuki Tanaka, Mina Okochi

    Journal of Bioscience and Bioengineering   2024.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2023.11.007

    researchmap

  • Screening of EWI-2-Derived Peptides for Targeting Tetraspanin CD81 and Their Effect on Cancer Cell Migration Reviewed

    Thanawat Suwatthanarak, Kei Usuba, Kotomi Kuroha, Masayoshi Tanaka, Mina Okochi

    Biomolecules   2023.3

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/biom13030510

    researchmap

  • Designable peptides on graphene field-effect transistors for selective detection of odor molecules

    Chishu Homma, Mirano Tsukiiwa, Hironaga Noguchi, Masayoshi Tanaka, Mina Okochi, Hideyuki Tomizawa, Yoshiaki Sugizaki, Atsunobu Isobayashi, Yuhei Hayamizu

    Biosensors and Bioelectronics   224   115047 - 115047   2023.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.bios.2022.115047

    researchmap

  • Volatile Organic Compound Detection by Graphene Field-Effect Transistors Functionalized with Fly Olfactory Receptor Mimetic Peptides Reviewed

    Tharatorn Rungreungthanapol, Chishu Homma, Ken-ichi Akagi, Masayoshi Tanaka, Jun Kikuchi, Hideyuki Tomizawa, Yoshiaki Sugizaki, Atsunobu Isobayashi, Yuhei Hayamizu, Mina Okochi

    Analytical Chemistry   2023.2

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.analchem.3c00052

    researchmap

  • A peptide binding to the tetraspanin CD9 reduces cancer metastasis Reviewed International journal

    Thanawat Suwatthanarak, Kazuma Ito, Masayoshi Tanaka, Kei Sugiura, Ayuko Hoshino, Yoshitaka Miyamoto, Kenji Miyado, Mina Okochi

    Biomaterials Advances   146   213283 - 213283   2023.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    As an organizer of multi-molecular membrane complexes, the tetraspanin CD9 has been implicated in a number of biological processes, including cancer metastasis, and is a candidate therapeutic target. Here, we evaluated the suppressive effects of an eight-mer CD9-binding peptide (CD9-BP) on cancer cell metastasis and its mechanisms of action. CD9-BP impaired CD9-related functions by adversely affecting the formation of tetraspanin webs-networks composed of CD9 and its partner proteins. The anti-cancer metastasis effect of CD9-BP was evidenced by the in vitro inhibition of cancer cell migration and invasion as well as exosome secretion and uptake, which are essential processes during metastasis. Finally, using a mouse model, we showed that CD9-BP reduced lung metastasis in vivo. These findings provide insight into the mechanism by which CD9-BP inhibits CD9-dependent functions and highlight its potential application as an alternative therapeutic nano-biomaterial for metastatic cancers.

    DOI： 10.1016/j.bioadv.2023.213283

    PubMed

    researchmap

  • A GFET Nitrile Sensor Using a Graphene‐Binding Fusion Protein

    Abubaker Mohamed, Hironaga Noguchi, Mirano Tsukiiwa, Chen Chen, Rachel Heath, M. Qadri E. Mubarak, Takumi Komikawa, Masayoshi Tanaka, Mina Okochi, Sam de Visser, Yuhei Hayamizu, Christopher Blanford

    Advanced Functional Materials   32 ( 46 )   2207669 - 2207669   2022.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/adfm.202207669

    researchmap

  • Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer. International journal

    Kazuki Takahashi, Shunsuke Chida, Thanawat Suwatthanarak, Mikiko Iida, Min Zhang, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Takao Yasui, Yoshinobu Baba, Akihide Hibara, Mina Okochi, Manabu Tokeshi

    Lab on a chip   22 ( 16 )   2971 - 2977   2022.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

    DOI： 10.1039/d2lc00224h

    PubMed

    researchmap

  • Sensitive and specific capture of polystyrene and polypropylene microplastics using engineered peptide biosensors. International journal

    Hyunjeong Woo, Seung Hyun Kang, Yejin Kwon, Yonghyun Choi, Jiwon Kim, Don-Hyung Ha, Masayoshi Tanaka, Mina Okochi, Jin Su Kim, Han Koo Kim, Jonghoon Choi

    RSC advances   12 ( 13 )   7680 - 7688   2022.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Owing to increased environmental pollution, active research regarding microplastics circulating in the ocean has attracted significant interest in recent times. Microplastics accumulate in the bodies of living organisms and adversely affect them. In this study, a new method for the rapid detection of microplastics using peptides was proposed. Among the various types of plastics distributed in the ocean, polystyrene and polypropylene were selected. The binding affinity of the hydrophobic peptides suitable for each type of plastic was evaluated. The binding affinities of peptides were confirmed in unoxidized plastics and plasma-oxidized plastics in deionised or 3.5% saline water. Also, the detection of microplastics in small animals' intestine extracts were possible with the reported peptide biosensors. We expect plastic-binding peptides to be used in sensors to increase the detection efficiency of microplastics and potentially help separate microplastics from seawater.

    DOI： 10.1039/d1ra08701k

    PubMed

    researchmap

  • Surface glycan targeting for cancer nano-immunotherapy

    Yonghyun Choi, Jiwon Kim, Jayoung Chae, Joohye Hong, Jongjun Park, Eunseo Jeong, Hayoung Kim, Masayoshi Tanaka, Mina Okochi, Jonghoon Choi

    JOURNAL OF CONTROLLED RELEASE   342   321 - 336   2022.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jconrel.2022.01.004

    Web of Science

    researchmap

  • Peptide-modified substrate enhances cell migration and migrasome formation. International journal

    Shogo Saito, Masayoshi Tanaka, Soichiro Tatematsu, Mina Okochi

    Materials science & engineering. C, Materials for biological applications   131   112495 - 112495   2021.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Extracellular vesicles (EVs) are cell-to-cell communication tools. Migrasomes are recently discovered microscale EVs formed at the rear ends of migrating cells, and thus are suggested to be involved in communicating with neighboring cells. In cell culture, peptide scaffolds on substrates have been used to demonstrate cellular function for regenerative medicine. In this study, we evaluated peptide scaffolds, including cell penetrating, virus fusion, and integrin-binding peptides, for their potential to enable the formation of migrasome-like vesicles. Through structural and functional analyses, we confirmed that the EVs formed on these peptide-modified substrates were migrasomes. We further noted that the peptide interface comprising cell-penetrating peptides (pVEC and R9) and virus fusion peptide (SIV) have superior properties for enabling cell migration and migrasome formation than fibronectin protein, integrin-binding peptide (RGD), or bare substrate. This is the first report of migrasome formation on peptide-modified substrates. Additionally, the combination of 95% RGD and 5% pVEC peptides provided a functional interface for effective migrasome formation and desorption of cells from the substrate via a simple ethylenediaminetetraacetic acid treatment. These results provide a functional substrate for the enhancement of migrasome formation and functional analysis.

    DOI： 10.1016/j.msec.2021.112495

    PubMed

    researchmap

  • Enrichment of membrane curvature-sensing proteins from Escherichia coli using spherical supported lipid bilayers.

    Masayoshi Tanaka, Yu Ueno, Takahiro Miyake, Takahiro Sakuma, Mina Okochi

    Journal of bioscience and bioengineering   133 ( 2 )   98 - 104   2021.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Bacteria display dynamically organized curved membrane structures, especially during cell division. The importance of membrane curvature-sensing (MCS) proteins for the recognition and regulation of biological membrane morphologies has predominately been investigated in eukaryotic cells. Recently, a technique for screening MCS proteins from solutions that contain peripheral membrane proteins was developed, and MCS protein candidates were identified from mammalian cells. The technique uses differently sized spherical supported lipid bilayers (SSLBs), which consist of spherical SiO2 particles covered with a lipid bilayer. To discriminate between proteins possessing the MCS property, SSLBs with the same surface area were used in a comparative sedimentation assay with shotgun proteome analysis. In this study, to prove that the technique could be applied to other samples, MCS proteins in Escherichia coli were investigated. Through a comparative proteomic study, 35 and 47 proteins were enriched as candidate MCS proteins preferentially bound to SSLBs of 100 nm and 1000 nm, respectively. Among the identified MCS candidate proteins, FtsZ and SecA were further examined for their MCS properties using the two SSLB sizes, which revealed a high binding affinity for the low membrane curvature (large SSLB). This is the first study to explore MCS proteins in prokaryotic cells and the MCS property of the SecA protein. The results demonstrate a method to enrich MCS proteins that could be utilized to better elucidate membrane dynamics and protein function expression on curved membrane structures in prokaryotic cells.

    DOI： 10.1016/j.jbiosc.2021.10.003

    PubMed

    researchmap

  • Synthesis of near-infrared absorbing triangular Au nanoplates using biomineralisation peptides. International journal

    Masayoshi Tanaka, Mirei Hayashi, Lucien Roach, Yuka Kiriki, Tetsuya Kadonosono, Takahiro Nomoto, Nobuhiro Nishiyama, Jonghoon Choi, Kevin Critchley, Stephen D Evans, Mina Okochi

    Acta biomaterialia   131   519 - 531   2021.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Triangular Au nanoplates (TrAuNPls) possessing strong plasmonic properties can be used as photothermal agents in cancer therapy. However, the preparation of such controlled morphologies typically requires harsh synthetic conditions. Biomolecules offer an alternative route to developing biocompatible synthetic protocols. In particular, peptides offer a novel route for inorganic synthesis under ambient conditions. Herein, using the previously isolated peptide, ASHQWAWKWE, for Au nanoparticle (AuNP) synthesis, the conditions for preparing TrAuNPls via a one-pot synthetic process of mixing HAuCl4 and peptides at room temperature were investigated to effectively obtain particles possessing near-infrared absorbance for non-invasive optical diagnosis and phototherapy. By adjusting the peptide concentration, the size and property of TrAuNPls were controlled under neutral pH conditions. The synthesised particles showed potential as photothermal therapeutic agents in vitro. In addition, peptide characterisation using B3 derivatives revealed the importance of the third amino acid histidine in morphological regulation and potential circular Au nanoplates (AuNPl) synthesis with ASEQWAWKWE and ASAQWAWKWE peptides. These findings provide not only an easy and green synthetic method for TrAuNPls and circular AuNPls, but also some insight to help elucidate the regulation of peptide-based nanoparticle synthesis for use in cancer therapy. STATEMENT OF SIGNIFICANCE: : Biological molecules have received increasing attention as a vehicle to synthesise inorganic materials with specific properties under ambient conditions; particularly, short peptides have the potential to control the synthesis of nanoscale materials with tailored functions. Here, the application of a previously isolated peptide was assessed in synthesising Au nanoparticles containing decahedral and triangular nanoplates with near-infrared absorbance. The size and absorbance peaks of the triangular nanoplates observed were peptide concentration-dependent. In addition, these fine-tuned triangular nanoplates exhibited potential as a phototherapeutic agent. Moreover, the peptide derivatives indicated the possibility of synthesising circular nanoplates. These findings may offer insight into development of new techniques for synthesising functional nanoparticles having biological applications using non-toxic molecules under mild conditions. stituted in the original B3 peptide is underlined.

    DOI： 10.1016/j.actbio.2021.06.010

    PubMed

    researchmap

  • Assemblies of bi-functional peptides on pyrolytic graphite for cell adhesion

    Soichiro Tatematsu, Tomoko Ohnishi, Shogo Saito, Masayoshi Tanaka, Yuhei Hayamizu, Mina Okochi

    Biochemical Engineering Journal   170   107988 - 107988   2021.6

    　More details

    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bej.2021.107988

    researchmap

  • Inhibition of cancer-cell migration by tetraspanin CD9-binding peptide. International journal

    Thanawat Suwatthanarak, Masayoshi Tanaka, Yoshitaka Miyamoto, Kenji Miyado, Mina Okochi

    Chemical communications (Cambridge, England)   57 ( 40 )   4906 - 4909   2021.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    A CD9-binding peptide (RSHRLRLH), screened from EWI-2, was characterized, and its effect on cellular migration and invasion was evaluated. As CD9 protein is overexpressed in cancer cells and plays an important role in cellular migration, the CD9-binding peptide preferentially inhibited the migration of cancer cells. Unlike conventional antiproliferative drugs, this CD9-binding peptide is promising as a novel precision antimigratory agent for cancer therapeutics.

    DOI： 10.1039/d1cc01295a

    PubMed

    researchmap

  • Microfluidic-based capture and release of cancer-derived exosomes via peptide-nanowire hybrid interface. International journal

    Thanawat Suwatthanarak, Ivan Adiyasa Thiodorus, Masayoshi Tanaka, Taisuke Shimada, Daiki Takeshita, Takao Yasui, Yoshinobu Baba, Mina Okochi

    Lab on a chip   21 ( 3 )   597 - 607   2021.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Cancer-derived circulating exosomes or nanoscale extracellular vesicles are emerging biomarkers for disease detection and treatment because of their cell-specific constituents and unique intercellular pathways. For efficient exosome isolation from bio-fluids, the design of high-affinity nanointerfaces is of great importance in the development of miniaturized systems for the collection of exosomes. Herein, we report peptide-functionalized nanowires as a biorecognition interface for the capture and release of cancer-derived exosomes within a microfluidic channel. Based on the amino-acid sequence of EWI-2 protein, a partial peptide that bound to the CD9 exosome marker and thus targeted cancer exosomes was screened. Linkage of the exosome-targeting peptide with a ZnO-binding sequence allowed one-step and reagent-free peptide modification of the ZnO nanowire array. As a result of peptide functionalization, the exosome-capturing ability of ZnO nanowires was significantly improved. Furthermore, the captured exosomes could be subsequently released from the nanowires under a neutral salt condition for downstream applications. This engineered surface that enhances the nanowires' efficiency in selective and controllable collection of cancer-derived exosomes provides an alternative foundation for developing microfluidic platforms for exosome-based diagnostics and therapeutics.

    DOI： 10.1039/d0lc00899k

    PubMed

    researchmap

  • Proteomic Exploration of Membrane Curvature Sensors Using a Series of Spherical Supported Lipid Bilayers. International journal

    Masayoshi Tanaka, Takumi Komikawa, Kentaro Yanai, Mina Okochi

    Analytical chemistry   92 ( 24 )   16197 - 16203   2020.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Membrane curvature-sensing (MCS) proteins recognize and regulate the morphologies of biological membranes. As these proteins lack characteristic sequence motifs in their primary structure, they are not instantly recognizable by genomic databases. Overcoming this technological challenge toward the agile identification of new proteins can promote the elucidation of membrane morphological regulation. Here, for the selective identification of MCS proteins, comparative proteomic analysis was performed using different sizes of the spherical supported lipid bilayer (SSLB), which consists of spherical SiO2 particles covered with a lipid bilayer. Because of the presence of SiO2 core, the curvature of the surrounding membrane is well-controlled and stable even on a micron scale. To prove this concept, known membrane curvature-sensing protein domains, Bin/Amphiphysin/Rvs (BAR) and Epsin N-terminal homology (ENTH), were evaluated by performing a binding assay using SSLBs, and the preferential binding to the highly curved membrane was confirmed. Peripheral membrane proteins obtained from normal human dermal fibroblast (NHDF) and human breast cancer (MDA-MB-231) cells were used in shotgun proteomic analysis, and 786 and 949 proteins were identified from SSLBs as lipid membrane binders, respectively. Statistical quantitative analyses of proteins detected from each SSLB with a different size revealed 118 candidate proteins, including 23 proteins unique to MDA-MB-231 cells, as membrane curvature sensors, including some previously reported curvature sensors. Functional clustering analysis based on the KEGG orthology database revealed that the protein-binding property to specific high or low membrane curvature correlated with their functions. Further investigation of candidate proteins will lead to the identification of new MCS proteins as well as cancer biomarkers.

    DOI： 10.1021/acs.analchem.0c04039

    PubMed

    researchmap

  • Peptide array-based inhibition ELISA for evaluating antigenicity in infant formulas.

    Chisato Kubo, Masaki Kurimoto, Masayoshi Tanaka, Hiroshi Ochi, Fumiaki Abe, Mina Okochi

    Journal of bioscience and bioengineering   130 ( 4 )   374 - 381   2020.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    With increased awareness among consumers regarding food safety and security, food allergen control has become an indispensable requirement in the food industry. Although several methods for detecting allergens in food products are available, highly sensitive techniques are required. In this study, we developed a technique named as peptide array-based inhibition enzyme-linked immunosorbent assay (ELISA), Pep-iEIA, for evaluating antigenicity and detecting cow's milk antigen in infant formula products, using a peptide array consisting of a series of overlapping peptides found in allergenic milk proteins. Pep-iEIA was used to examine five cow's milk-based infant formulas with different degrees of hydrolyzation, and the assay offered both more sensitive detection and detailed analysis of remaining antigenic peptides in allergen compared to conventional ELISA. The antigenicity level of the allergenic peptides identified using Pep-iEIA was confirmed by surface plasmon resonance assay. We believe that Pep-iEIA will be highly useful for antigenicity evaluation of dairy products consumed by infants and patients with cow's milk allergy.

    DOI： 10.1016/j.jbiosc.2020.06.010

    PubMed

    researchmap

  • Machine learning-driven electronic identifications of single pathogenic bacteria. International journal

    Shota Hattori, Rintaro Sekido, Iat Wai Leong, Makusu Tsutsui, Akihide Arima, Masayoshi Tanaka, Kazumichi Yokota, Takashi Washio, Tomoji Kawai, Mina Okochi

    Scientific reports   10 ( 1 )   15525 - 15525   2020.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    A rapid method for screening pathogens can revolutionize health care by enabling infection control through medication before symptom. Here we report on label-free single-cell identifications of clinically-important pathogenic bacteria by using a polymer-integrated low thickness-to-diameter aspect ratio pore and machine learning-driven resistive pulse analyses. A high-spatiotemporal resolution of this electrical sensor enabled to observe galvanotactic response intrinsic to the microbes during their translocation. We demonstrated discrimination of the cellular motility via signal pattern classifications in a high-dimensional feature space. As the detection-to-decision can be completed within milliseconds, the present technique may be used for real-time screening of pathogenic bacteria for environmental and medical applications.

    DOI： 10.1038/s41598-020-72508-3

    PubMed

    researchmap

  • Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides. International journal

    Rosie M Jarrald, Aw W Liang Alvin, Andrea E Rawlings, Masayoshi Tanaka, Mina Okochi, Sarah S Staniland

    Bioconjugate chemistry   31 ( 8 )   1981 - 1994   2020.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Using protein and peptide additives to direct the crystallization of inorganic materials is a very attractive and environmentally friendly strategy to access complex and sometimes inaccessible mineral phases. CoPt is a very desirable high-magnetoanisotropic material in its L10 phase, but this is acquired by annealing at high temperatures which is incompatible with delicate nanomaterial assembly. Previous studies identified one peptide with high affinity to CoPt and four peptides with high affinity to FePt L10 phase nanoparticles (NPs) through phage display biopanning selection. While synthesis mediated by these peptides offered a small degree of L10 character to the NPs, they do not have the magnetoanistropy required for applications. In this study, we improve the activity of peptide directed crystallization by designing second generation peptides. We use the five literature sequences (LS) to probe the binding affinity deeper through dissection (alanine scanning), reduction (truncations), and substitution of the LS to find key amino acids and motifs. This is performed using a SPOT peptide array, importantly probing interactions at three stages of NP formation: with precursor, during synthesis, and with NPs. We found four universal features: 1) the importance of basic residues, particularly lysine flanking both ends of the sequence; 2) the importance of methionine; 3) shorter sequences show higher affinity than longer ones; and 4) acidic residues have a negative impact on binding with aspartic acid less favorable than glutamic acid. However, an acidic amino acid benefits, presumably to balance charge. The short motif KSLS had high affinity in all assays. Three sequences were selected from the screening, and three sequences were designed from the rules above. These were used to mediate a green synthesis of CoPt nanoparticles. The screened peptides mediated the formation of NPs with improved coercivity (90-110 Oe) compared to the LS (30-80 Oe), while the designed peptides facilitated formation of CoPt NPs with the highest coercivity (109 to 132 Oe), representing a massive improvement on L10 character. This result along with deeper insight this methodology brings offers vast potential for the future.

    DOI： 10.1021/acs.bioconjchem.0c00348

    PubMed

    researchmap

  • Peptide-Functionalized Quantum Dots for Rapid Label-Free Sensing of 2,4,6-Trinitrotoluene. Reviewed International journal

    Takumi Komikawa, Masayoshi Tanaka, Abiral Tamang, Stephen D Evans, Kevin Critchley, Mina Okochi

    Bioconjugate chemistry   31 ( 5 )   1400 - 1407   2020.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Explosive compounds, such as 2,4,6-trinitrotoluene (TNT), pose a great concern in terms of both global public security and environmental protection. There are estimated to be hundreds of TNT contaminated sites all over the world, which will affect the health of humans, wildlife, and the ecosystem. Clearly, the ability to detect TNT in soils, water supplies, and wastewater is important for environmental studies but also important for security, such as in ports and boarders. However, conventional spectroscopic detection is not practical for on-site sensing because it requires sophisticated equipment and trained personnel. We report a rapid and simple chemical sensor for TNT by using TNT binding peptides which are conjugated to fluorescent CdTe/CdS quantum dots (QDs). QDs were synthesized in the aqueous phase, and the peptide was attached directly to the surface of the QDs by using thiol groups. The fluorescent emission from the QDs was quenched in response to the addition of TNT. The response could even be observed by the naked eye. The limit of detection from fluorescence spectroscopic measurement was estimated to be approximately 375 nM. In addition to the rapid response (within a few seconds), selective detection was demonstrated. We believe this label-free chemical sensor contributes to progress for the on-site explosive sensing.

    DOI： 10.1021/acs.bioconjchem.0c00117

    PubMed

    researchmap

  • A bioinspired peptide matrix for the detection of 2,4,6-trinitrotoluene (TNT). Reviewed International journal

    Takumi Komikawa, Masayoshi Tanaka, Kentaro Yanai, Benjamin R G Johnson, Kevin Critchley, Takeshi Onodera, Stephen D Evans, Kiyoshi Toko, Mina Okochi

    Biosensors & bioelectronics   153   112030 - 112030   2020.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bios.2020.112030

    Scopus

    PubMed

    researchmap

  • In situ bioimaging of Lactobacillus by photoluminescence of MoS2 Reviewed

    Tezuka, Sayaka, Seki, Takakazu, Ohnishi, Tomoko, Noguchi, Hironaga, Tanaka, Masayoshi, Okochi, Mina, Hayamizu, Yuhei

    2D MATERIALS   7 ( 2 )   2020.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1088/2053-1583/ab602b

    Web of Science

    researchmap

  • Array-Based Screening of Silver Nanoparticle Mineralization Peptides. Reviewed International journal

    Masayoshi Tanaka, Shogo Saito, Reo Kita, Jaehee Jang, Yonghyun Choi, Jonghoon Choi, Mina Okochi

    International journal of molecular sciences   21 ( 7 )   2020.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions.

    DOI： 10.3390/ijms21072377

    PubMed

    researchmap

  • Screening and characterisation of CdTe/CdS quantum dot-binding peptides for material surface functionalisation. International journal

    Thanawat Suwatthanarak, Masayoshi Tanaka, Taisuke Minamide, Andrew J Harvie, Abiral Tamang, Kevin Critchley, Stephen D Evans, Mina Okochi

    RSC advances   10 ( 14 )   8218 - 8223   2020.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Quantum dots (QDs) are promising nanomaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple and bio-friendly QD modification. Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. Consequently, the dual-functional peptides could mediate anti-CD9 antibody functionalisation onto CdTe/CdS QD surface; CD9 protein imaging of cancer cells was also demonstrated. Our proposed peptides offer an effective vehicle for QD surface functionalisation in biological applications.

    DOI： 10.1039/d0ra00460j

    PubMed

    researchmap

  • Quartz Crystal Microbalance Sensor Based on Peptide Anchored Single-Walled Carbon Nanotubes for Highly Selective TNT Explosive Detection

    Jin Wang, Masayoshi Tanaka, Mina Okochi

    2020 IEEE SENSORS   2020

    　More details

    Language：English   Publishing type：Research paper (international conference proceedings)  

    Web of Science

    researchmap

  • Peptide-modified microelectrode-based potentiometric device for 2,4,6-trinitrotoluene molecule detection Reviewed

    Wang, J., Yatabe, R., Onodera, T., Tanaka, M., Okochi, M., Toko, K.

    Sensors and Materials   31 ( 3 )   2609 - 2617   2019

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.18494/SAM.2019.2385

    Scopus

    researchmap

  • Rational screening of biomineralisation peptides for colour-selected one-pot gold nanoparticle syntheses Reviewed

    Tanaka, M., Takahashi, Y., Roach, L., Critchley, K., Evans, S.D., Okochi, M.

    Nanoscale Advances   1 ( 1 )   71 - 75   2019

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry ({RSC})  

    DOI： 10.1039/c8na00075a

    Scopus

    researchmap

  • Peptide screening from a phage display library for benzaldehyde recognition Reviewed

    Tanaka, M., Minamide, T., Takahashi, Y., Hanai, Y., Yanagida, T., Okochi, M.

    Chemistry Letters   48 ( 8 )   978 - 981   2019

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1246/cl.190318

    Scopus

    researchmap

  • Analyzing peptide adsorption states via nanowire-employed infrared spectrometry

    Hiroki Naito, Takao Yasui, Taisuke Shimada, Nobutaka Shioya, Takafumi Shimoaka, Masayoshi Tanaka, Kazuki Nagasima, Mina Okochi, Takeshi Yanagida, Takeshi Hasegawa, Yoshinobu Baba

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   1206 - 1207   2019

    　More details

    Publishing type：Research paper (international conference proceedings)  

    Scopus

    researchmap

  • Enhanced Tubulation of Liposome Containing Cardiolipin by MamY Protein from Magnetotactic Bacteria. Reviewed International journal

    Masayoshi Tanaka, Thanawat Suwatthanarak, Atsushi Arakaki, Benjamin R G Johnson, Stephen D Evans, Mina Okochi, Sarah S Staniland, Tadashi Matsunaga

    Biotechnology journal   13 ( 12 )   e1800087   2018.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/biot.201800087

    Scopus

    PubMed

    researchmap

  • Identification of Individual Bacterial Cells through the Intermolecular Interactions with Peptide-Functionalized Solid-State Pores. Reviewed International journal

    Makusu Tsutsui, Masayoshi Tanaka, Takahiro Marui, Kazumichi Yokota, Takeshi Yoshida, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, Mina Okochi, Tomoji Kawai

    Analytical chemistry   90 ( 3 )   1511 - 1515   2018.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant (ΔfliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level. These results provide a novel concept of utilizing weak intermolecular interactions as a recognition probes for single-cell microbial identification.

    DOI： 10.1021/acs.analchem.7b04950

    PubMed

    researchmap

  • Multimodal resistive pulse analysis using a low-aspect-ratio nanopore

    Makusu Tsutsui, Takeshi Yoshida, Masayoshi Tanaka, Kazumichi Yokota, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Mina Okochi, Takashi Washio, Tomoji Kawai

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2   754 - 757   2018

    　More details

    Publishing type：Research paper (international conference proceedings)  

    Scopus

    researchmap

  • An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2, 4, 6-Trinitrotoluene Explosives. Reviewed International journal

    Jin Wang 0016, Sanyang Du, Takeshi Onodera, Rui Yatabe, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

    Sensors   18 ( 12 )   4461 - 4461   2018

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/s18124461

    Scopus

    PubMed

    researchmap

  • Identifying Single Viruses Using Biorecognition Solid-State Nanopores Reviewed International journal

    Arima, A., Harlisa, I.H., Yoshida, T., Tsutsui, M., Tanaka, M., Yokota, K., Tonomura, W., Yasuda, J., Taniguchi, M., Washio, T., Okochi, M., Kawai, T.

    Journal of the American Chemical Society   140 ( 48 )   16834 - 16841   2018

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/jacs.8b10854

    Scopus

    PubMed

    researchmap

  • Selective detections of single-viruses using solid-state nanopores Reviewed

    Arima, A., Tsutsui, M., Harlisa, I.H., Yoshida, T., Tanaka, M., Yokota, K., Tonomura, W., Taniguchi, M., Okochi, M., Washio, T., Kawai, T.

    Scientific Reports   8 ( 1 )   16305   2018

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-018-34665-4

    Scopus

    researchmap

  • Screening of bacteria-binding peptides and one-pot ZnO surface modification for bacterial cell entrapment Reviewed International journal

    Tanaka, M., Harlisa, I.H., Takahashi, Y., Ikhsan, N.A., Okochi, M.

    RSC Advances   8 ( 16 )   8795 - 8799   2018

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c7ra12302g

    Web of Science

    Scopus

    PubMed

    researchmap

  • Highly Selective Rational Design of Peptide-Based Surface Plasmon Resonance Sensor for Direct Determination of 2,4,6-trinitrotoluene (TNT) Explosive Reviewed

    Wang, J., Muto, M., Yatabe, R., Tahara, Y., Onodera, T., Tanaka, M., Okochi, M., Toko, K.

    Sensors and Actuators, B: Chemical   264   279 - 284   2018

    　More details

    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.snb.2018.02.075

    Web of Science

    Scopus

    researchmap

  • Screening of peptide probe binding to particulate matter with a high metal content Reviewed International journal

    Tanaka, M., Alvin, A.W.L., Okochi, M.

    RSC Advances   8 ( 11 )   5953 - 5959   2018

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c7ra13290e

    Web of Science

    Scopus

    PubMed

    researchmap

  • Screening of peptides associated with adhesion and aggregation of Lactobacillus rhamnosus GG in vitro Reviewed

    Mina Okochi, Tomoya Sugita, Yuji Asai, Masayoshi Tanaka, Hiroyuki Honda

    BIOCHEMICAL ENGINEERING JOURNAL   128   178 - 185   2017.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bej.2017.10.004

    Web of Science

    researchmap

  • Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody Reviewed International journal

    Mina Okochi, Masaki Muto, Kentaro Yanai, Masayoshi Tanaka, Takeshi Onodera, Jin Wang, Hiroshi Ueda, Kiyoshi Toko

    ACS COMBINATORIAL SCIENCE   19 ( 10 )   625 - 632   2017.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acscombsci.7b00035

    Web of Science

    PubMed

    researchmap

  • Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system. Reviewed International journal

    Mina Okochi, Shinji Koike, Masayoshi Tanaka, Hiroyuki Honda

    Biosensors & bioelectronics   93   32 - 39   2017.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bios.2016.11.013

    Web of Science

    PubMed

    researchmap

  • Characterization of particulate matter binding peptides screened from phage display. Reviewed

    Aw Wei Liang Alvin, Masayoshi Tanaka, Mina Okochi

    Journal of bioscience and bioengineering   123 ( 5 )   621 - 624   2017.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2016.12.014

    Web of Science

    PubMed

    researchmap

  • Array-based functional peptide screening and characterization of gold nanoparticle synthesis. Reviewed International journal

    Masayoshi Tanaka, Shun Hikiba, Kiyoto Yamashita, Masaki Muto, Mina Okochi

    Acta biomaterialia   49   495 - 506   2017.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.actbio.2016.11.037

    Web of Science

    PubMed

    researchmap

  • Rational Design of Peptide-Functionalized Surface Plasmon Resonance Sensor for Specific Detection of TNT Explosive. Reviewed International journal

    Jin Wang 0016, Masaki Muto, Rui Yatabe, Takeshi Onodera, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

    Sensors   17 ( 10 )   2249 - 2249   2017

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/s17102249

    Web of Science

    PubMed

    researchmap

  • Development of a tactical screening method to investigate the characteristics of functional peptides Reviewed

    Akiko Kume, Mina Okochi, Kazunori Shimizu, Yasuko Yoshida, Hiroyuki Honda

    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING   21 ( 1 )   119 - 127   2016.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12257-015-0523-0

    Web of Science

    researchmap

  • A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria Reviewed

    Sayuri Arai, Mina Okochi, Kazunori Shimizu, Taizo Hanai, Hiroyuki Honda

    BIOTECHNOLOGY AND BIOENGINEERING   113 ( 1 )   112 - 119   2016.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bit.25707

    Web of Science

    PubMed

    researchmap

  • Rapid colorimetric antibody detection using a dual-function peptide probe for silver nanoparticle aggregation and antibody recognition Reviewed

    Mina Okochi, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda

    Analytical Sciences   32 ( 1 )   93 - 97   2016.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    researchmap

  • Efficient capturing of circulating tumor cells using a magnetic capture column and a size-selective filter Reviewed

    Shuhei Yamamoto, Jiahui Fei, Mina Okochi, Kazunori Shimizu, Akiko Yusa, Naoto Kondo, Hiroji Iwata, Hayao Nakanishi, Hiroyuki Honda

    BIOPROCESS AND BIOSYSTEMS ENGINEERING   38 ( 9 )   1693 - 1704   2015.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00449-015-1412-9

    Web of Science

    PubMed

    researchmap

  • Design of a dual-function peptide probe as a binder of angiotensin II and an inducer of silver nanoparticle aggregation for use in label-free colorimetric assays Reviewed

    Mina Okochi, Masashi Kuboyama, Masayoshi Tanaka, Hiroyuki Honda

    TALANTA   142   235 - 239   2015.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.talanta.2015.04.054

    Web of Science

    PubMed

    researchmap

  • Effects of the properties of short peptides conjugated with cell-penetrating peptides on their internalization into cells Reviewed

    Ryo Matsumoto, Mina Okochi, Kazunori Shimizu, Kei Kanie, Ryuji Kato, Hiroyuki Honda

    SCIENTIFIC REPORTS   5   2015.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep12884

    Web of Science

    researchmap

  • A molecular peptide beacon for IgG detection Reviewed

    M. Okochi, T. Sugita, M. Tanaka, H. Honda

    RSC ADVANCES   5 ( 112 )   91988 - 91992   2015

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/c5ra15174k

    Web of Science

    researchmap

  • Effect of Vascular Formed Endothelial Cell Network on the Invasive Capacity of Melanoma Using the In Vitro 3D Co-Culture Patterning Model Reviewed

    Shuhei Yamamoto, Michael Masakuni Hotta, Mina Okochi, Hiroyuki Honda

    PLOS ONE   9 ( 7 )   e103502   2014.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0103502

    Web of Science

    PubMed

    researchmap

  • Development of a New Rapid Isolation Device for Circulating Tumor Cells (CTCs) Using 3D Palladium Filter and Its Application for Genetic Analysis Reviewed

    Akiko Yusa, Makoto Toneri, Taisuke Masuda, Seiji Ito, Shuhei Yamamoto, Mina Okochi, Naoto Kondo, Hiroji Iwata, Yasushi Yatabe, Yoshiyuki Ichinosawa, Seichin Kinuta, Eisaku Kondo, Hiroyuki Honda, Fumihito Arai, Hayao Nakanishi

    PLOS ONE   9 ( 2 )   e88821   2014.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0088821

    Web of Science

    PubMed

    researchmap

  • Magnetic force-based cell patterning for evaluation of the effect of stromal fibroblasts on invasive capacity in 3D cultures Reviewed

    Mina Okochi, Taku Matsumura, Hiroyuki Honda

    BIOSENSORS & BIOELECTRONICS   42   300 - 307   2013.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bios.2012.09.067

    Web of Science

    PubMed

    researchmap

  • Cell behavior observation and gene expression analysis of melanoma associated with stromal fibroblasts in a three-dimensional magnetic cell culture array Reviewed

    Mina Okochi, Taku Matsumura, Shuhei Yamamoto, Eiichi Nakayama, Kowichi Jimbow, Hiroyuki Honda

    BIOTECHNOLOGY PROGRESS   29 ( 1 )   135 - 142   2013.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/btpr.1642

    Web of Science

    PubMed

    researchmap

  • Personalized assessment of oxidative cellular damages associated with diabetes using erythrocytes adhesion assay Reviewed

    Mina Okochi, Yuki Sakai, Yayoi Isaji, Hiroshi Nagasaki, Yoji Hamada, Hiroyuki Honda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   112 ( 6 )   635 - 637   2011.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2011.08.003

    Web of Science

    PubMed

    researchmap

  • Screening of peptides with a high affinity for ZnO using spot-synthesized peptide arrays and computational analysis Reviewed

    Mina Okochi, Masafumi Ogawa, Chiaki Kaga, Tomoya Sugita, Yasuyuki Tomita, Ryuji Kato, Hiroyuki Honda

    ACTA BIOMATERIALIA   6 ( 6 )   2301 - 2306   2010.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.actbio.2009.12.025

    Web of Science

    PubMed

    researchmap

  • On-chip type cation-exchange chromatography with ferrocene-labeled anti-hemoglobin antibody and electrochemical detector for determination of hemoglobin A(1c) level Reviewed

    Tsuyoshi Tanaka, Kojiro Izawa, Mina Okochi, Tae-Kyu Lim, Shugo Watanabe, Manabu Harada, Tadashi Matsunaga

    ANALYTICA CHIMICA ACTA   638 ( 2 )   186 - 190   2009.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aca.2009.02.016

    Web of Science

    PubMed

    researchmap

  • Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens Reviewed

    Naoki Matsumoto, Mina Okochi, Miyoko Matsushima, Akiko Ogawa, Tomokazu Takase, Yasuko Yoshida, Mitsuo Kawase, Ken-ichi Isobe, Tsutomu Kawabe, Hiroyuki Honda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   107 ( 3 )   324 - 330   2009.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2008.10.014

    Web of Science

    PubMed

    researchmap

  • 医療機器被覆のための機能性ペプチドの探索

    蟹江 慧, 加藤 竜司, 趙 瑛梓, 成田 裕司, 大河内 美奈, 本多 裕之

    化学工学会 研究発表講演要旨集   2009   1036 - 1036   2009

    　More details

    Language：Japanese   Publisher：公益社団法人 化学工学会  

    DOI： 10.11491/scej.2009f.0.1036.0

    researchmap

  • A Motif Detection and Classification Method for Peptide Sequences Using Genetic Programming Reviewed

    Yasuyuki Tomita, Ryuji Kato, Mina Okochi, Hiroyuki Honda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   106 ( 2 )   154 - 161   2008.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1263/jbb.106.154

    Web of Science

    PubMed

    researchmap

  • Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain Reviewed

    Mina Okochi, Shigeyuki Nomura, Chiaki Kaga, Hiroyuki Honda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   371 ( 1 )   85 - 89   2008.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2008.04.019

    Web of Science

    PubMed

    researchmap

  • Effect of global transcriptional regulators related to carbohydrate metabolism on organic solvent tolerance in Escherichia coli Reviewed

    Mina Okochi, Masaki Kurimoto, Kazunori Shimizu, Hiroyuki Honda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 4 )   389 - 394   2008.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1263/jbb.105.389

    Web of Science

    PubMed

    researchmap

  • Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays Reviewed

    Mina Okochi, Hiroki Hayashi, Akira Ito, Ryuji Kato, Yasuaki Tamura, Noriyuki Sato, Hiroyuki Honda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 3 )   198 - 203   2008.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1263/jbb.105.198

    Web of Science

    PubMed

    researchmap

  • Electrochemical detection of HbA(1c), a maker for diabetes, using a flow immunoassay system Reviewed

    Tsuyoshi Tanaka, Shoko Tsukube, Kojiro Izawa, Mina Okochi, Tae-Kyu Lim, Shugo Watanabe, Manabu Harada, Tadashi Matsunaga

    BIOSENSORS & BIOELECTRONICS   22 ( 9-10 )   2051 - 2056   2007.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bios.2006.09.008

    Web of Science

    PubMed

    researchmap

  • Enhanced activity of 3 alpha-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (L)-lactate in aqueous phase of biphasic systems for reductive production of steroids Reviewed

    Mina Okochi, Izumi Nakagawa, Takeshi Kobayashi, Shuhei Hayashi, Shintaro Furusaki, Hiroyuki Honda

    JOURNAL OF BIOTECHNOLOGY   128 ( 2 )   376 - 382   2007.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiotec.2006.10.001

    Web of Science

    PubMed

    researchmap

  • Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli Reviewed

    Mina Okochi, Masaki Kurimoto, Kazunori Shimizu, Hiroyuki Honda

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   73 ( 6 )   1394 - 1399   2007.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-006-0624-y

    Web of Science

    PubMed

    researchmap

  • Development of an electrochemical antifouling system for seawater cooling pipelines of power plants using titanium Reviewed

    Hitoshi Wake, Hiromichi Takahashi, Toshihiro Takimoto, Hirokazu Takayanagi, Kinichi Ozawa, Hideo Kadoi, Mina Okochi, Tadashi Matsunaga

    BIOTECHNOLOGY AND BIOENGINEERING   95 ( 3 )   468 - 473   2006.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bit.21022

    Web of Science

    PubMed

    researchmap

  • Application of bioinformatic prediction for supporting high-throughput experiments in proteome Reviewed

    Ryuji Kato, Yasuyuki Tomita, Mina Okochi, Hiroyuki Honda

    PROGRESS ON POST-GENOME TECHNOLOGIES   26 - 29   2006

    　More details

    Language：English   Publishing type：Research paper (international conference proceedings)  

    Web of Science

    researchmap

  • Disinfection of microorganisms by use of electrochemically regenerated periodate Reviewed

    M Okochi, H Yokokawa, TK Lim, T Taguchi, H Takahashi, H Yokouchi, T Kaiho, A Sakuma, T Matsunaga

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   71 ( 10 )   6410 - 6413   2005.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/AEM.71.10.6410-6413.2005

    Web of Science

    PubMed

    researchmap

  • Electrochemical probe for on-chip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde Reviewed

    M Okochi, H Ohta, T Tanaka, T Matsunaga

    BIOTECHNOLOGY AND BIOENGINEERING   90 ( 1 )   14 - 19   2005.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bit.20313

    Web of Science

    PubMed

    researchmap

  • Development of an automated water toxicity biosensor using Thiobacillus ferrooxidans for monitoring cyanides in natural water for a water filtering plant Reviewed

    M Okochi, K Mima, M Miyata, Y Shinozaki, S Haraguchi, M Fujisawa, M Kaneko, T Masukata, T Matsunaga

    BIOTECHNOLOGY AND BIOENGINEERING   87 ( 7 )   905 - 911   2004.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/bit.20193

    Web of Science

    PubMed

    researchmap

  • Kinetics and binding sites for interaction of the prefoldin with a group II chaperonin - Contiguous non-native substrate and chaperonin binding sites in the archaeal prefoldin Reviewed

    M Okochi, T Nomura, T Zako, T Arakawa, R Iizuka, H Ueda, T Funatsu, M Leroux, M Yohda

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 30 )   31788 - 31795   2004.7

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M402889200

    Web of Science

    PubMed

    researchmap

  • Chaperonin-mediated stabilization and ATP-triggered release of semiconductor nanoparticles. Reviewed International journal

    Daisuke Ishii, Kazushi Kinbara, Yasuhiro Ishida, Noriyuki Ishii, Mina Okochi, Masafumi Yohda, Takuzo Aida

    Nature   423 ( 6940 )   628 - 32   2003.6

    　More details

    Language：English  

    Various properties of semiconductor nanoparticles, including photoluminescence and catalytic activity, make these materials attractive for a range of applications. As nanoparticles readily coagulate and so lose their size-dependent properties, shape-persistent three-dimensional stabilizers that enfold nanoparticles have been exploited. However, such wrapping approaches also make the nanoparticles insensitive to external stimuli, and so may limit their application. The chaperonin proteins GroEL (from Escherichia coli) and T.th ('T.th cpn', from Thermus thermophilus HB8) encapsulate denatured proteins inside a cylindrical cavity; after refolding, the encapsulated proteins are released by the action of ATP inducing a conformational change of the cavity. Here we report that GroEL and T.th cpn can also enfold CdS semiconductor nanoparticles, giving them high thermal and chemical stability in aqueous media. Analogous to the biological function of the chaperonins, the nanoparticles can be readily released from the protein cavities by the action of ATP. We expect that integration of such biological mechanisms into materials science will open a door to conceptually new bioresponsive devices.

    DOI： 10.1038/nature01663

    PubMed

    researchmap

  • Pyrococcus prefoldin stabilizes protein-folding intermediates and transfers them to chaperonins for correct folding Reviewed

    M Okochi, T Yoshida, T Maruyama, Y Kawarabayasi, H Kikuchi, M Yohda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   291 ( 4 )   769 - 774   2002.3

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1006/bbrc.2002.6523

    Web of Science

    PubMed

    researchmap

  • 1L1515 Interactions among Pyrococcus prefoldin, substrate protein and chapcronin

    Zako T., Iizuka R., Okochi M., Ueno T., Yohda M., Funatsu T.

    Seibutsu Butsuri   42 ( 2 )   S71   2002

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.42.S71_2

    researchmap

  • Molecular chaperone system of archaea

    Yohda M., Yoshida T., Okochi M., Usui K., Maruyama T.

    Seibutsu Butsuri   40   S159   2000

    　More details

    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.40.S159_4

    CiNii Books

    researchmap

▼display all

MISC

▼display all

Industrial property rights

  • 細胞特異的ペプチド及びその用途

    本多 裕之, 大河内 美奈, 加藤 竜司, 蟹江 慧, 趙 瑛梓, 成田 裕司

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2014-241948  Date applied：2014.11

    Announcement no：特開2015-044057  Date announced：2015.3

    J-GLOBAL

    researchmap

  • 蛍光プローブ及び検出方法

    本多 裕之, 大河内 美奈, 杉田 智哉

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2013-239263  Date applied：2013.11

    Announcement no：特開2015-098457  Date announced：2015.5

    J-GLOBAL

    researchmap

  • 乳酸菌の付着性を誘導するペプチド及びその用途

    本多 裕之, 大河内 美奈, 杉田 智哉

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：JP2010063478  Date applied：2010.8

    Announcement no：WO2011-021524  Date announced：2011.2

    J-GLOBAL

    researchmap

  • 酸化ストレス評価法及びその用途

    本多 裕之, 大河内 美奈, 伊佐治 弥生, 濱田 洋司, 長崎 弘

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：JP2010060347  Date applied：2010.6

    Announcement no：WO2010-147206  Date announced：2010.12

    J-GLOBAL

    researchmap

  • アレルギー疾患を診断するための方法

    本多 裕之, 大河内 美奈, 加藤 竜司, 松本 直樹, 川部 勤, 松島 充代子, 吉田 安子, 高瀬 智和, 吉良 茂樹

    　More details

    Applicant：国立大学法人名古屋大学, 倉敷紡績株式会社

    Application no：特願2011-506158  Date applied：2010.3

    Patent/Registration no：特許第5728678号  Date issued：2015.4

    J-GLOBAL

    researchmap

  • アレルギー疾患の診断方法

    本多 裕之, 大河内 美奈, 加藤 竜司, 松本 直樹, 川部 勤, 松島 充代子, 吉田 安子, 高瀬 智和, 吉良 茂樹

    　More details

    Applicant：国立大学法人名古屋大学, 日本碍子株式会社

    Application no：JP2010055456  Date applied：2010.3

    Announcement no：WO2010-110454  Date announced：2010.9

    J-GLOBAL

    researchmap

  • アレルゲンのエピトープ又はその候補の検出方法及びその利用

    本多 裕之, 大河内 美奈, 加藤 竜司, 松本 直樹, 川部 勤, 松島 充代子, 吉田 安子, 高瀬 智和

    　More details

    Applicant：国立大学法人名古屋大学, 日本碍子株式会社

    Application no：JP2009005997  Date applied：2009.11

    Announcement no：WO2010-052939  Date announced：2010.5

    J-GLOBAL

    researchmap

  • 細胞特異的ペプチド及びその用途

    本多 裕之, 大河内 美奈, 加藤 竜司, 蟹江 慧, 趙 瑛梓, 成田 裕司

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2009-195728  Date applied：2009.8

    Announcement no：特開2011-046637  Date announced：2011.3

    Patent/Registration no：特許第5664992号  Date issued：2014.12

    J-GLOBAL

    researchmap

  • 細胞特異的ペプチド及びその用途

    本多 裕之, 大河内 美奈, 加藤 竜司, 蟹江 慧, 趙 瑛梓, 成田 裕司

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2009-195728  Date applied：2009.8

    Announcement no：特開2011-046637  Date announced：2011.3

    J-GLOBAL

    researchmap

  • 機能性ペプチドを表すルールの抽出法、機能性ペプチドの設計法及び調製法、ポリペプチド又はポリペプチド含有組成物の評価法、並びに機能性ペプチド

    本多 裕之, 大河内 美奈, 加藤 竜司, 加賀 千晶, 蟹江 慧, 長岡 利

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2009-071498  Date applied：2009.3

    Announcement no：特開2010-222300  Date announced：2010.10

    J-GLOBAL

    researchmap

  • がん細胞挙動評価モデル及びその用途

    本多 裕之, 大河内 美奈, 千賀 威, 高野 翔

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2008-286173  Date applied：2008.11

    Announcement no：特開2010-110272  Date announced：2010.5

    J-GLOBAL

    researchmap

  • 酸化ストレス評価法及びその用途

    本多 裕之, 大河内 美奈, 伊佐治 弥生

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2007-332529  Date applied：2007.12

    Announcement no：特開2009-156624  Date announced：2009.7

    J-GLOBAL

    researchmap

  • 高親和性ペプチドの設計法及び調製法、並びに高親和性ペプチド

    本多 裕之, 大河内 美奈, 加賀 千晶, 冨田 康之, 加藤 竜司

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2006-243883  Date applied：2006.9

    Announcement no：特開2008-063285  Date announced：2008.3

    J-GLOBAL

    researchmap

  • 細胞死誘導ペプチド、MAP化細胞死誘導ペプチド、細胞死誘導剤及び抗癌剤

    本多 裕之, 大河内 美奈, 加藤 竜司, 中西 麻里

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2006-048016  Date applied：2006.2

    Announcement no：特開2007-223958  Date announced：2007.9

    Patent/Registration no：特許第5034005号  Date issued：2012.7

    J-GLOBAL

    researchmap

  • 細胞死誘導ペプチド、MAP化細胞死誘導ペプチド、細胞死誘導剤及び抗癌剤

    本多 裕之, 大河内 美奈, 加藤 竜司, 中西 麻里

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2006-048016  Date applied：2006.2

    Announcement no：特開2007-223958  Date announced：2007.9

    J-GLOBAL

    researchmap

  • 水生生物の電気化学的制御方法及び防汚方法

    和気 仁志, 中山 鶴雄, 松永 是, 大河内 美奈, 高橋 美奈子, 中村 徳幸

    　More details

    Applicant：ぺんてる株式会社, 松永 是

    Application no：特願2004-322818  Date applied：2004.11

    Announcement no：特開2005-088007  Date announced：2005.4

    Patent/Registration no：特許第3668976号  Date issued：2005.4

    J-GLOBAL

    researchmap

  • 水生生物の電気化学的制御方法及び防汚方法

    和気 仁志, 中山 鶴雄, 松永 是, 大河内 美奈, 高橋 美奈子, 中村 徳幸

    　More details

    Applicant：松永 是

    Application no：特願2004-322818  Date applied：2004.11

    Announcement no：特開2005-088007  Date announced：2005.4

    J-GLOBAL

    researchmap

  • 水生生物の電気化学的制御方法及び防汚方法

    和気 仁志, 中山 鶴雄, 松永 是, 大河内 美奈, 高橋 美奈子, 中村 徳幸

    　More details

    Applicant：ぺんてる株式会社, 松永 是

    Application no：特願平8-083123  Date applied：1996.3

    Announcement no：特開平9-248554  Date announced：1997.9

    J-GLOBAL

    researchmap

▼display all

Awards

  • 第30回生物工学技術賞

    2021   日本生物工学会   「ペプチドアレイ」を基盤とする低抗原性生理活性ペプチドの製造

    栗本昌樹, 久保智里, 越智浩, 本多裕之, 大河内美奈

    　More details

  • 女性賞

    2016   化学工学会  

    　More details

  • 生物工学会奨励賞（斉藤賞）

    2012   日本生物工学会   ナノ磁性粒子を用いたテクノロジーの開発と応用

    　More details

    Country：Japan

    researchmap

  • 第４３回東海化学工業会賞

    2008  

    　More details

    Country：Japan

    researchmap

  • 化学素材研究開発振興財団記念基金グラント

    2006   一般財団法人バイオインダストリー協会   超高熱性子最近由来プレフォルディン導入による微生物の有機溶媒耐性機構の解析

    　More details

Research Projects

  • 細胞遊走により形成される生体膜小胞ミグラソームを介した炎症反応の誘導

    Grant number：24K01266  2024.4 - 2027.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    大河内 美奈, 齊藤 彰吾

    　 More details

    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

    researchmap

  • Design of tetraspanin-regulating peptides to inhibit cancer invasion and metastasis

    Grant number：22K19913  2022.6 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Okochi Mina

    　 More details

    Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）

    Invasion and metastasis are hallmarks of cancer biology, complex sequential and interrelated processes that lead to the formation of distant secondary tumors. In this study, we designed CD9-binding peptides based on the amino acid sequence of EWI-2, a regulatory molecule that interacts with tetraspanin CD9. The CD9-binding peptide inhibits CD9-mediated tetraspanin web formation, exosome secretion and uptake, and cell migration and invasion in cancer cells. These findings provide insight into how CD9-binding peptide inhibits CD9-dependent functions and highlight its potential application as an alternative therapeutics for metastatic cancers.

    researchmap

  • バイオアクティブペプチド界面を利用したミグラソームの機能解析

    Grant number：21H01726  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    大河内 美奈, 田中 祐圭

    　 More details

    Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）

    researchmap

  • Exploration of membrane curvature sensing protein using a series of spherical supported lipid bilayers

    Grant number：21H01725  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    　 More details

    Grant amount：\17550000 （ Direct Cost: \13500000 、 Indirect Cost：\4050000 ）

    researchmap

  • Design of peptide interface for functional analysis of migrasomes formed at the rear of migrating cells

    Grant number：18K18970  2018.6 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    Okochi Mina

    　 More details

    Grant amount：\6240000 （ Direct Cost: \4800000 、 Indirect Cost：\1440000 ）

    Migrasomes are biological membrane vesicles about 1 μm in size that form on the adhesive surface at the rear of cells migration. In this study, we screened functional peptides that can stably capture migrasomes from various biological membrane binding peptides by using peptide arrays. The technique can synthesize various peptide sequences in parallel by spot synthesis process. The cell culture interface using the peptides identified herein has no negative effect on cell adhesion, migration, proliferation and vesicle formation activity, and capture migrasomes stably in comparison to the scaffolding protein of fibronectin. It is expected that the process of forming migrasomes in migrating cells and its function can be elucidated by using this functional interface.

    researchmap

  • Construction of cell-electrode biointerface with self-assembling peptides

    Grant number：18H01795  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Okochi Mina

    　 More details

    Grant amount：\17160000 （ Direct Cost: \13200000 、 Indirect Cost：\3960000 ）

    Two-dimensional materials are attracting attention as next-generation electronics materials that can acquire signals at high speed and with high sensitivity, and the construction of biointerfaces that do not impair material properties is a challenge. In this study, we explored peptides that exhibit affinity for cells and electronic materials in order to design biodevice interfaces using the self-assembling peptides. By simply preparing the bifunctional peptides and dropping their solution, a cell recognition interface was constructed on the surface of the specific material by self-assembly. It was suggested that this peptide interface can be used for a variety of cell types, imaging and detection, and can also be used to capture a variety of detection targets by changing the peptide sequence to suit the target object.

    researchmap

  • Virus sensing based on infectivity using biological membrane structure control protein

    Grant number：16K14488  2016.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Tanaka Masayoshi, Evans Stephen, Chritchley Kevin

    　 More details

    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    We investigated the development of a new electrochemical virus sensor with virus infection as an indicator by covering a conductive element with a biological membrane that mimics cell membranes. According to the original research plan, various functional peptides including sensor element binding peptide, biological membrane binding peptide and virus binding peptide were identified and applied for the sensor development. Using these peptides with pore sensor electrochemical devise, it was successfully shown that one influenza virus can be detected with the potential of the species identification. By carrying out detailed comparative analysis between active and inactive viruses, new reliable virus sensing devices without false positive detection can be developed in near future.

    researchmap

  • Development of bioactive cell interface using peptides and nano-materials

    Grant number：15H04192  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Okochi Mina

    　 More details

    Grant amount：\17680000 （ Direct Cost: \13600000 、 Indirect Cost：\4080000 ）

    Two-dimensional materials such as graphene, an individual layer of graphite with only an atom thickness, have attracted great attention due to its superior electrical properties. Along with these properties, increasing exploration on the potential applications of graphene and its derivatives are being utilized in many areas including electronic and energy storage devices, advanced materials, biomedicine, and biotechnology. In this study, self-assembling peptides have been focused as the non-covalent functionalization biointerface material, and peptides self-assembling to the surface of thin layer of pyropytic graphite was designed and bi-functionalized by conjugating to the peptide sequences showing affinity to cell membrane.

    researchmap

  • Design of immune tolerance inducing peptide using the peptide array analysis technique

    Grant number：15K14230  2015.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    OKOCHI Mina

    　 More details

    Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）

    Peptide microarray is useful for the antibody epitope mapping since it enables high-throughput binding interaction analysis of medical serological samples. In this study, milk allergy mouse model was made by four times immunization of casein with alum adjuvant and heterovalent IgE epitope was analyzed using the milk peptide microarray that composed peptide library of six milk protein amino acid sequences. Using the milk allergy mouse sera-IgE sensitized basophilic leukemia cells, design of short epitope peptides was investigated for inhibition of allergic reaction by allergen addition. When the short epitope peptides was administrated to milk allergy mouse, allergic responses such as scratching and rectal temperature decrease were suppressed after allergen administration. Therefore, it was indicated that the short epitope peptides could be used as the personalized medicine in oral immunotherapy and it would help safe administration of allergens by suppressing allergic reactions.

    researchmap

  • Chemistry integrated circuit and its application to portable biomedical equipment

    Grant number：25220906  2013.5 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    Nakazato Kazuo

    　 More details

    Grant amount：\171210000 （ Direct Cost: \131700000 、 Indirect Cost：\39510000 ）

    Chemistry integrated circuits, where several chemical reactions are controlled and detected, have been developed. New analog CMOS (complementary metal-oxide semiconductor) circuits based on current-mode operation were proposed to realize low-power and high-accuracy sensors. Multimodal sensor arrays with potentiometric, amperometric, impedimetric, and photometric 64x64 sensors have been designed and fabricated. MEMS (Micro Electro Mechanical Systems) were integrated on CMOS circuits to control the chemical reactions and the transport of chemical reagents. Stand-alone and hand-held equipment has been developed, and single bacterium was successfully detected using dielectrophoresis and impedance measurement.

    researchmap

  • Establishment of screening method on intracellular functional peptides by cell array technology

    Grant number：25289292  2013.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    HONDA HIROYUKI

    　 More details

    Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）

    Cell death inducing peptide was explored from CPP(cell penetrating peptide) conjugated peptide library and effective cell death of MCF-7 was observed for newly designed octamer peptide, LNLIWKLF, in which fifth amino acid, serine, of the original peptide was substituting to tryptophan and it’s activity was approximately 4 times higher compared with that of the original peptide. When serine residue was replaced to hydrophobic amino acid, F, V, and Y, cell death activity was also further increased. In the case of cell-cycle targeting peptide, newly designed peptide with higher activity was also successfully designed. In addition, minimized peptide library for screening was studied based on 4 amino acid groups categorized from 20 amino acids. Using 2 sets of 256 tetramer peptides, IL-2 binding peptide was assigned and high binding role was identified by principal component analysis. It was found that acquired role could be utilized for screening of octamer functional peptides.

    researchmap

  • Research on single cell function analysis by magnetic force-based cell patterning

    Grant number：21360400  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    HONDA Hiroyuki, OKOCHI Mina, KATO Ryuji, SHIKIDA Mitsuhiro

    　 More details

    Grant amount：\17680000 （ Direct Cost: \13600000 、 Indirect Cost：\4080000 ）

    Single cell analysis including cell-cell interaction was studied. Based on magnetic force based cell patterning, gene expression analysis, and image analysis of single cell, the following basic analytical methods were established ; 1) simple magnetic labeling method, 2) few cell functional analysis on peptide array, 3) cell function assessment by image analysis, and 4) single cell function analysis of tumor cell.

    researchmap

  • 赤血球を用いた酸化ストレス簡易モニタリングデバイスの開発

    Grant number：21656214  2009 - 2010

    日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

    大河内 美奈

    　 More details

    Grant amount：\3200000 （ Direct Cost: \3200000 ）

    酸化ストレスは糖尿病合併症をはじめ多くの疾患の発症に関与している.糖尿病合併症のうち動脈硬化,心筋梗塞,脳梗塞等の大血管症の発症には,持続的な高血糖や代謝異常に由来する酸化ストレスの亢進が関与するため,血糖値管理のみでは十分に予防できない.本研究では,酸化ストレスに起因する血管障害を反映する新規指標として赤血球の表面電荷の変化に着目し,微量血液により簡便で日常的に測定できるPoint of Care Testing(POCT)の開発を目指した.簡易血糖値センサを利用し,赤血球の表面電荷の変化に伴う電極への付着量を電気化学的に検出した.正電位印加後の電極を赤血球懸濁液中に浸漬した後,電子メディエータ溶液中でサイクリックボルタンメトリーを行った.酸化剤としてt-butyl hydroperoxide処理した赤血球を測定した結果,酸化剤濃度や処理時間に伴い電流応答が変化した.また,高グルコース負荷を行った赤血球においても,電流応答が変化した.これより,in vitro試験において酸化ストレスを受けた赤血球の影響を電気化学的に測定できることが示唆された.そこで,糖尿病患者から静脈血をヘパリン採血して測定した結果,健常者群と比較して患者群では電流値の上昇がみられ,得られた電流密度は長期的な血糖コントロールを反映するHbA1cと相関する傾向が得られた.以上のことから,赤血球を用いて簡便に酸化ストレスによる血管障害リスクを測定できることが示唆された.

    researchmap

  • MAGNETIC FORCE-BASED-DROPLET-HANDLING SYSTEM FOR SINGLE CELL DIGITAL PCR ANALYSIS

    Grant number：20360371  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    OKOCHI Mina, HONDA Hiroyuki, KATO Ryuji, SHIKIDA Mitsuhiro, MIZUTANI Takashi

    　 More details

    Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）

    A droplet-based single cell analysis system was developed employing magnetic force-based droplet-handling system. The droplets containing magnetic beads were magnetically controlled and coalesced with another droplet in the oil phase, and all the reactions for RT-PCR including single cell manipulation, cell lysis, cDNA synthesis, and PCR amplification steps were integrated on-chip and applied for detection of cancer cells.

    researchmap

  • Peptideinformatics-establishment of newly screening and design method of functional peptide-

    Grant number：17206082  2005 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    HONDA Hiroyuki, OKOCHI Mina, KATO Ryuji, ITO Akira

    　 More details

    Grant amount：\49920000 （ Direct Cost: \38400000 、 Indirect Cost：\11520000 ）

    Peptides have attracted attention for their ability to regulate a variety of cellular events by interacting with receptors on the cell surface. A large number of biomaterials that conjugate functional peptides have been developed for medicine, drug delivery, tissue engineering, bio-imaging, and food additives. However, identifying functional peptides by exhaustive screening that covers all the possible combinations of 20 amino acids is terribly time-consuming.
    In this research, “Peptideinformatics" was proposed, which is combined new peptide screening method combined with computational analysis. The method is based on the concept that screening efficiency can be enhanced from even limited data by use of a model derived from computational analysis that serves as a guide to screening and combining it with subsequent repeated experiments. Here we focus on cell adhesion peptides as a model application of this peptide-screening strategy. Cell adhesion peptides were screened by use of a cell-based assay of a peptide array. Starting with the screening data obtained from a limited, random 5-mer library (643 sequences), a rule regarding structural characteristics of cell adhesion peptides was extracted by fuzzy neural network (FMN) analysis. According to this rule, peptides with nnfavored residues in certain positions that led to inefficient binding were eliminated from the random sequences. In the restricted, second random library (273 sequences), the yield of cell adhesion peptides having an adhesion rate more than 1.5-fold to that of the basal array-support was significantly high (31%) compared to the unrestricted random library (20%). In the restricted third library (50 sequences), the yield of cell adhesion peptides increased to 84%. We conclude that a repeated cycle of experiments screening limited numbers of peptides can be assisted by the rule-extracting feature of FNN.

    researchmap

  • Acquisition of organic solvent tolerance using molecular chaperone in E.coli

    Grant number：17560688  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    OKOCHI Mina, HONDA Hiroyuki

    　 More details

    Grant amount：\3500000 （ Direct Cost: \3500000 ）

    The molecular mechanisms in organic solvent tolerance of microorganisms have become increasingly important in whole-cell bioprocesses involving an organic solvent-aqueous phase for the bioconversion of substrates that have relatively low solubility in water. In the present study, organic solvent tolerant mutants were constructed by introducing molecular chaperones.
    (1) Prefoldin from the hyperthermophilic archaea increased organic solvent tolerance.
    Molecular stability of extremophile enzymes generally reflects the growth temperatire of parent microorganisms and positively correlated with the stability in the presence of denaturing enzymes including organic solvents. Expression of prefoldin from hyperthermophilic archaeum, Pyrococcus horikoshii OT3 (PhPfd) resulted in increased organic solvent tolerance in Escherichia coli cells. The cells overexpressing PhPfd could grow continuously after addition of organic solvent mixture of octane and hexane at 10% volume. The intracellular level of cells overexpressing PhPfd was kept lower than the control cells. Archaeal prefoldin consists of two species of subunits and exists as a hexamer of α2β4 in jellyfish like structure. Overexpression of PhPFD truncation mutants that have low chaperone activities resulted in decreased organic solvent tolerance. Therefore, it was shown that molecular chaperone activity of prefoldin resulted in increased organic solvent tolerance in E.coli.
    (2) Construction of organic solvent tolerant mutants by overexpression of upregulated genes in transcriptional analysis when addition of organic solvents.
    Overexpression of manXYZ that was selected from gene expression profile was shown to increase organic solvent tolerance level of E.coli JA300 strain. The cells could grow in an organic solvent mixture of hexane and cyclohexane at 10% volume. Since overexpression of ManXYZ in cell membrane changed cell surface property to obtain higher affinity to hydrocarbons, this strain would be useful in whole-cell bioprocesses in biphasic systems. In the analysis of global regulatory genes related to glucose catabolism, knockout mutants of cyclic AMP receptor protein (crp) and adenylate cyclase (cyaA) was found to increase their tolerance to organic solvents. In the transcriptional analysis of a crp knockout mutant, genes related to molecular chaperones were upregulated.

    researchmap

  • Nano-screening of marine symbiotic consortium for useful bio-materials and their analyses

    Grant number：15360437  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    TAKEYAMA Haruko, TANAKA Tuyoshi, ARAKAKI Atsushi, MARUYAMA Tadashi

    　 More details

    Grant amount：\14900000 （ Direct Cost: \14900000 ）

    Recently, marine microbial consortium has been paid attention as new targets for screening of biologically active agents and so on. However, most of bacteria in environment are unculturable and unidentified. In this study, we aimed to have gene resources and develop the micro-assay system for small number of cells which are directly collected. investigate biodiversity, species identification of bacteria associated with marine sponge and coral, which are well known to produce several biologically active agents. Furthermore, methods for their efficient collection from host cells and genome amplification, and micro-screening system have been developed.
    Microbial cells were sorted by using flow cytometer and their 16SrDNA analyses were performed before/after cell sorting. 16SrDNA analysis showed that various kinds of bacteria which were not found in the sample not sorted were observed in the sorted cell samples. Those cells are not enough number of cells for construction of genome library. They are subjected to genome amplification by MDA method using phai-29 DNA polymerase. It was confirmed that enough genome DNA was amplified and amplicons less than 10 Kb were not chimeric when the model microbes were used. Furthermore, amine-coated nano-beads showed high performance in DNA extraction/collection.
    In order to micro-screening of useful materials, several devises were constructed. Cell-capture devise constructed showed 90% cell capture without any non-specific adsorption. Furthermore, micro-capillary devise for micro-screening was also constructed. In the devise, gradient of chemicals introduced into capillary was observed. This showed that it is possible to examine biological effect of target chemicals under the various of their concentration.

    researchmap

  • マイクロ・ナノシステムによる遺伝子解析のための核酸濃縮技術の開発

    Grant number：15760585  2003 - 2004

    日本学術振興会  科学研究費助成事業  若手研究(B)

    大河内 美奈

    　 More details

    Grant amount：\2700000 （ Direct Cost: \2700000 ）

    本研究は、マクロからミクロへのインターフェースとして電気泳動の原理を利用し核酸を濃縮する方法の開発を目的とした。具体的には、mLおよびサブmLオーダーのサンプル量から核酸を電気泳動により微少な透析膜上へ補修する技術の開発を行った。具体的には、サンプルを入れる容器に透析膜を被覆した微少スポットに、電気泳動の原理を利用して核酸を濃縮することを検討した。平成15年度において検討した核酸の濃縮条件を踏まえて96穴プレート用および電気泳動チップ用の装置を設計し、電気泳動により透析膜上に核酸を濃縮するシステムを試作した。核酸濃縮に用いた電気泳動システムを用いて、電気泳動の指標として用いられる色素であるキシレンシアノール、オレンジG、ブロモフェノールブルーなどを用いて濃縮条件を検討した。次に、濃縮用核酸ターゲットとして各種長さのssDNA、dsDNAをモデルターゲットとして電圧制御で核酸の濃縮条件を検討し、蛍光色素染色による評価を行った。試作した装置において、電解液や電解条件などの濃縮条件や核酸濃縮に用いることが可能な核酸の量や濃度などの条件を求め、試作した装置において核酸濃縮が可能であることが示された。また、電流制御においても同様に核酸の濃縮が可能であった。
    以上のことから、電気泳動の原理を利用し、微少な透析膜上に核酸を捕集・濃縮することが可能であると示された。これより、感染症の特定遺伝子やmRNA等、サンプル中に微量に存在する核酸を濃縮して解析することが可能となると考えられる。

    researchmap

  • プレフォルディンとII型シャペロニンによるタンパク質フォールディング機構の解明

    Grant number：14037216  2002

    日本学術振興会  科学研究費助成事業  特定領域研究

    養王田 正文, 大河内 美奈

    　 More details

    Grant amount：\4000000 （ Direct Cost: \4000000 ）

    我々はII型シャペロニンとそのCo-chaperoneであるプレフォルディンによるタンパク質フォールディング機構の解明を目標に研究を進めている。具体的には超好熱性古細菌Pyrococcus horikoshii由来シャペロニン(PhCPN)及びプレフォルディン(PhPFD)を組み換え体として大腸菌で生産し、in vitroで機能解析を行っている。本年度はプレフォルディンの基質及びシャペロニン結合部位の同定、シャペロニンとの協調機構に関する研究を行った。
    メタン生成古細菌由来プレフォルディンのX線結晶構造から各サブユニットのN及びC末端が変性タンパク質の結合に関与していることが示唆されている。そこで、PhPFDの各サブユニットの末端を除去し変異体を作成し、機能への寄与を解析した。PhPFDβC末8残基欠損体で基質タンパク質結合能力が低下し、5残基欠損体では低下が見られなかった事から、PhPFDβのC末6-8残基部分の存在が、基質タンパク質をアレストする認識に、重要であることが示された。また、βのN末欠損体でも基質タンパク質結合能力が低下した。PhPFDαは、末端部分欠損体では基質タンパク質結合能力が低下しないが、コイル構造部分まで深く欠失させた変異体(tc24tn17α)で結合能が失われていた。また、αC末8残基欠損、βC末5残基欠損したPhPFDは、基質タンパク質結合能の低下に影響がみられなかったがシャペロニンへの結合能が低下した。
    PhCPNとPhPFDとの協調機構を変性IPMDH(Thermus thermophilus由来イソプロピルリンゴ酸脱水素酵素)のリフォールディングにより解析したところ、PhCPN単独よりもPhPFDが共存した場合に高い効率でリフォールディングしたことから、PhPFDとPhCPNが協調してタンパク質フォールディングを行っていることが証明された。

    researchmap

  • Group II型シャペロニン及びその補因子プレフォルディンの構造解析

    Grant number：13033008  2002

    日本学術振興会  科学研究費助成事業  特定領域研究

    養王田 正文, 大河内 美奈

    　 More details

    Grant amount：\3600000 （ Direct Cost: \3600000 ）

    我々は、超好熱性古細菌由来Group II型シャペロニン、プレフォルディンを用いて、構造学的にGroup II型シャペロニンのBuild-in Lid開閉機構及びシャペロニンとプレフォルディンの協調機構の解明を目的に研究を行っている。
    様々なヌクレオチド存在下で、野生型αシャペロニンにproteinase Kを作用させたところ、ATP, AMP-PNP存在下では分解が妨げられた。また、アピカルドメインの先端にTrp残基を導入した変異体を作成し、構造変化をその蛍光特性から追跡した。その結果、ATP, AMP-PNPと結合したシャペロニンにおいて蛍光の増大が観測された。これらの結果は、ATPとの結合に伴い、Built-in Lidが閉じることを示唆するものだと思われる。一方、リフォールディング活性を示さない変異体αシャペロニンを用いた際には、この構造変化を示す結果は得られなかった。Built-in LidのDeletion Mutantを作成したところ、変性タンパク質結合能は有していたが、タンパク質フォールディング活性は失われていた。このDeletion Mutantの結晶を得ることに成功した。
    プレフォルディンはWild型の結晶から良いDiffractionが得られないことから、βサブユニットのC末8残基欠失体の結晶化を試み20%PEG 4000、0.1M HEPES(pH7.7)の条件で結晶が得られた。しかし、この結晶からも構造解析に使える反射を得ることは出来なかった。シャペロニンとの共結晶の作成を試みたが結晶は得られていない。
    また、酵母由来プレフォルディンの各サブユニットの発現系を作成し、全てのサブユニットを組み込んだ同時生産系を構築した。

    researchmap

  • 特異的DNA配列を用いた非好熱性古細菌の探索

    Grant number：13750731  2001 - 2002

    日本学術振興会  科学研究費助成事業  若手研究(B)

    大河内 美奈

    　 More details

    Grant amount：\800000 （ Direct Cost: \800000 ）

    本研究は、特殊環境に生育しない古細菌の探索を目的とした。古細菌はこれまで高温、高塩濃度、高酸、高アルカリなどの極限環境中から単離され、その代表的な種において全ゲノム解析がなされている。古細菌が極限環境で生息するための機構について生化学的な解析が進められている他、産業上安定性の高い有用酵素の分離源といった面から注目されている。しかし極限環境ではない環境中に生息する古細菌についての知見は少ない。そこで、古細菌に特異的な16S rRNAの塩基配列をプローブとし、環境中に生息する古細菌の探索を行った。まず、プローブの有効性を確認するため、温泉(鹿児島県霧島温泉および長野県渋温泉)やその周辺の水、土壌について探索を行った。採取した水および土壌サンプルのゲノム抽出を行った後、古細菌およびバクテリアに特異的な16S rRNAプローブによりPCRを行った結果、半数以上の温泉サンプルについて増幅が認められたほか、水や土壌サンプルについても一部増幅が確認された。霧島温泉および渋温泉の温泉源は95℃以上の高温のものが多いことから、超好熱性および好熱性古細菌を中心として古細菌が生息していることが示唆された。次に、地下水や湧水について同様に検討を行った。関東および関西から入手した地下水3サンプル、東京都国分寺市の真姿の池湧水群において古細菌特異的プローブによる増幅がみられた。これより、16S rDNAシークエンス解析を進めている。以上のことから、古細菌が極限環境だけではなく身近な環境中に存在していることが示唆された。

    researchmap

  • 磁気微粒子を用いた自動遺伝子解析システムの開発

    Grant number：12019216  2000

    日本学術振興会  科学研究費助成事業  特定領域研究(A)

    養王田 正文, 細川 和生, 大河内 美奈

    　 More details

    Grant amount：\2000000 （ Direct Cost: \2000000 ）

    磁気微粒子を用いたDNAの分離精製機能を有する遺伝子組換え用ワークステーションはすでに市販されており,遺伝子組換えの様々な操作の自動化が可能である.しかし,電気泳動によるDNAの分離・精製を行うことができないため遺伝子解析や遺伝子組換えの全自動化は不可能であった.そこで,アガロースゲルによるDNAの分離・精製の自動化を実現するためにシステムの改造を行った.具体的には,電気泳動装置,CCDカメラを用いたゲル撮影装置,自動サンプル注入装置及びゲル回収装置の開発とそれらに伴うソフトの開発を行った.作製した装置(SX-8G plus)は,コンピューター上からのコマンドにより装置内を3次元的に自由に移動できる8連のノズルを有し,デスクトップには,マイクロタイタープレート,チップラック,試薬コンテナーの他に,自動開閉蓋式サーマルサイクラー,試薬をストックするための冷却ブロック,アガロースゲル電気泳動槽が配置されている.また,電気泳動用電源,ゲルの染色と脱色を自動で行うためのポンプ,ゲルを撮影するためのトランスイルミネーターとCCDカメラも本体に組み込まれている.SX-8G plusは,これまで困難とされていたアガロースゲル電気泳動,遺伝子断片の検出とゲルからの切り出しの自動化を可能とした.一般的に,XYZロボットはアガロースゲルのウエルの位置を合わせるのに十分な精度を有している.ロボットによるサンプルのアガロースゲルへのローディングが困難なのは,使用するチップやゲルの位置などの精度が低いためである.SX-8G plusでは,特別な二重になったコームを使用することで,チップやゲルの精度の低さによるローディングの失敗を防いでいる.ゲルの写真はCCDカメラで撮影されコンピューターに取りこまれる.目的のDNA断片をディスプレイ上でカーソルを用いて指定することにより,自動的に切り出すことが可能である.ゲルの切り出しはノズルにゲルカッターを装着しておこない,切り出したゲルはマイクロタイタープレートなの任意の位置に排出される.現在,この装置の性能評価を行っている.

    researchmap

▼display all