Organization
School of Engineering Professor
Title
Professor
External link
YAGI Tohru
News & Topics
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健常者の抑うつ症状を反映した脳波反応を同定 うつ病の早期発見や予防法の開発に期待
2023/10/27
Languages： Japanese
要点-健常者を対象に、風景・人物・動物などの写真を提示した時の脳波反応を計測-抑うつの症状が重いほど、写真提示に対する脳波反応の振幅が小さいことを発見-うつ病を早期発見する手法の開発に期待東海大学情報通信学部情報通信学科の中谷裕教講師、ならびに東
Degree
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Doctor of Engineering ( Nagoya University )
Research Interests
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Neural Engineering, Biomedical Engineering
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Artificial Vision
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Eye-gaze Interface
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EEG
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医用生体工学
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生体情報工学
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ロボット/システム工学
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視覚科学
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神経インタフェース
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人工眼
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人工視覚
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視線入力
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脳波
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BCI
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Brain Computer Interface
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Brain Machine Interface
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Visual Prosthesis
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BMI
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Biomedical Engineering
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Biological Information Processing
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Robotics & System Integration
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Vision
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Neural Interface
Research Areas
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Measurement engineering / Biomedical Engineering, Neural Engineering, Bio-MEMS, Biological Signal Processing
Education
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Nagoya University Graduate School of Engineering Dept. of Electro-Mechanical Engineering
1990 - 1996
Country： Japan
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University of Michigan Rackham Graduate School Bioengineering Program
1991 - 1992
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Nagoya University School of Engineering Dept. of Mechanical Engineering
1985 - 1990
Research History
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Institute of Science Tokyo School of Engineering Professor
2022
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Tokyo Institute of Technology School of Engineering Associate Professor
2016 - 2022
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Massachusetts Institute of Technology School of Engineering Fulbright Visiting Researcher
2012 - 2013
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Tokyo Institute of Technology Graduate School of Information Science and Engineering Associate Professor
2007 - 2016
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Tokyo Institute of Technology Graduate School of Information Science and Engineering Associate Professor
2005 - 2007
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Institute of Physical and Chemical Research Biomimetic Control Research Center Researcher
2004 - 2005
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The University of Tokyo Research Center for Advanced Science and Technology Visiting Researcher
2004 - 2005
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NIDEK Co., Ltd. Artiricial Vision Institute Research Director
2001 - 2004
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The Institute of Physical and Chemical Research Bio-mimetic Control Research Center Adjunct Research Scientist
2000 - 2004
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Nagoya University Graduate School of Engineering Assistant Professor
1998 - 2001
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Toyota National College of Technology Dept. of Information and Computer Engineering Adjunct Lecturer
1998 - 2000
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The Institute of Physical and Chemical Research Postdoctoral Research Scientist
1996 - 1998
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Nagoya University Graduate School of Engineering JSPS Researcher (DC2)
1994 - 1996
Professional Memberships
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計測自動制御学会
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日本工学教育協会
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生体医工学会
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電気学会
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IEEE-EMBS
Committee Memberships
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The Institute of Electrical Engineering of Japan (IEEJ) Chairman of Electronics, Information and Systems Society
2025
Committee type：Academic society
Papers
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Synthetic DNA nanopores for direct molecular transmission between lipid vesicles. International journal
Zugui Peng, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
Nanoscale 2024.6
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Effects of inserting ultrashort carbon nanotubes into lipid bilayers on membrane morphology
Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
Electronics and Communications in Japan 2024.6
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脂質二重膜への超短カーボンナノチューブ挿入が及ぼす膜形態への影響
Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 144 ( 5 ) 424 - 430 2024.5
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Ultrasound-Responsive Liposome-Encapsulated Gel Patches Drug Delivery Model
Kano Kajie, Shoichiro Kanno, Zugui Peng, Kenta Shimba, Takashi Shibata, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 144 ( 5 ) 465 - 466 2024.5
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生体適合性と電気的特性に優れた電極の開発に向けたカーボンナノチューブの細胞接着性に関する研究
Kittawat Wardcharoen, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 144 ( 5 ) 467 - 468 2024.5
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Yuto Okayasu, Yuya Shimomura, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 144 ( 5 ) 463 - 464 2024.5
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Synthetic DNA nanopores for direct molecular transmission between lipid vesicles
Zugui Peng, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
Nanoscale 2024
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A Study on Cell Culture Scaffolds using Magnetic Gel
Yuya Shimomura, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 143 ( 7 ) 714 - 715 2023.7
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A Study on DDS with High Response to Ultrasound using Metal Nanoparticle-encapsulated Liposomes
Yukine Tagai, Kano Kajie, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Takashi Shibata, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 143 ( 7 ) 712 - 713 2023.7
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Dimethyl Sulfoxide-Free Cryopreservation of Differentiated Human Neuronal Cells International journal
Kenji Yamatoya, Yuya Nagai, Naozumi Teramoto, Woojin Kang, Kenji Miyado, Kazuya Nakata, Tohru Yagi, Yoshitaka Miyamoto
Biopreservation and Biobanking 21 ( 6 ) 631 - 634 2023.2
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Control of Drug Release in Ultrasound-Responsive Liposome-Encapsulated Gel Patches.
Kano Kajie, Zugui Peng, Kenta Shimba, Takashi Shibata 0006, Yoshitaka Miyamoto, Tohru Yagi
MeMeA 1 - 5 2023
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Yoshitaka Miyamoto, Yumie Koshidaka, Katsutoshi Murase, Shoichiro Kanno, Hirofumi Noguchi, Kenji Miyado, Takeshi Ikeya, Satoshi Suzuki, Tohru Yagi, Naozumi Teramoto, Shuji Hayashi
Materials 15 ( 21 ) 7823 - 7823 2022.11
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Cryopreservation of undifferentiated and differentiated human neuronal cells International journal
Kenji Yamatoya, Yuya Nagai, Naozumi Teramoto, Woojin Kang, Kenji Miyado, Kazuya Nakata, Tohru Yagi, Yoshitaka Miyamoto
Regenerative Therapy 19 58 - 68 2022.3
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Development of a Battery Using a Hydrogel Network
Kohtaro Hongo, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022) 2022
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Transmembrane Carbon Nanotubes for Intracellular Stimulation
Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022) 2022
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Development of Variable-Elasticity Cell Scaffolds Using Magnetic Gels
Yuya Shimomura, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022) 2022
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Enhancement of the Response to Ultrasound by Encapsulating Metal Nanoparticles in Liposomes
Yukine Tagai, Kano Kajie, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Takashi Shibata, Tohru Yagi
2022 14TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2022) 2022
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Development of novel ECM which has stiffness difference based on hydrogel beads
Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
ELECTRONICS AND COMMUNICATIONS IN JAPAN 104 ( 3 ) 2021.9
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A Study of the Effects of Plasma Surface Treatment on Lipid Bilayers Self-Spreading on a Polydimethylsiloxane Substrate under Different Treatment Times. Reviewed International journal
Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
Langmuir : the ACS journal of surfaces and colloids 37 ( 36 ) 10732 - 10740 2021.8
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Effect of Magnetic Nanoparticle Internalization on Cell Density in Skeletal Muscle Tissue Reviewed
Yuji Kirihara, Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Kazunori Shimizu, Hiroyuki Honda, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 141 ( 7 ) 795 - 801 2021.7
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Functional analysis of liposomes containing Single-Walled Carbon Nanotubes (SWNTs) by fluorescence microscopy Reviewed
Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 141 ( 5 ) 620 - 626 2021
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Development of novel ECM which has stiffness difference based on hydrogel beads Reviewed
Hiromu Miyata, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 141 ( 5 ) 607 - 613 2021
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Formation of agarose-supported liposomes by polymer-assisted method toward biosensor Reviewed
Zugui Peng, Kentaro Wada, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 141 ( 5 ) 646 - 653 2021
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Preparation of Size-controlled Giant Vesicles Under Physiological Conditions. Reviewed International journal
Zugui Peng, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference 2020 2198 - 2201 2020.7
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Nanopore lipid bilayer formed by self-spreading method
Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
ELECTRONICS AND COMMUNICATIONS IN JAPAN 102 ( 12 ) 47 - 54 2019.12
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Preparation of giant lipobeads using a gel-assisted swelling method Reviewed
Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
BMEiCON 2019 - 12th Biomedical Engineering International Conference 2019.11
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Nanopore-spanning lipid bilayer formed by self-spreading method
Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
BMEiCON 2018 - 11th Biomedical Engineering International Conference 139 ( 10 ) 1146 - 1152 2019.1
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Effect of the amount of carbon nanotubes introduced into liposomes on membrane permeability
Shoichiro Kanno, Zugui Peng, Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
13TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON 2021) 2018
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Hydrogel-supported bilayers for studying membrane protein functions Reviewed
Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
BMEiCON 2017 - 10th Biomedical Engineering International Conference 2017- 1 - 4 2017.12
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Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture. Reviewed International journal
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
Cell medicine 9 ( 1-2 ) 35 - 44 2017.1
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Self-spreading method for forming lipid bilayer on a patterned agarose gel: Toward precise lipid bilayer patterning. Reviewed International journal
Kenta Shimba, Kazuma Shoji, Yoshitaka Miyamoto, Tohru Yagi
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2017 1877 - 1880 2017
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Development of a method for patterning hydrogel-supported bilayer
Shimba Kenta, Shoji Kazuma, Miyamoto Yoshitaka, Yagi Tohru
Transactions of Japanese Society for Medical and Biological Engineering 55 ( 5 ) 449 - 449 2017
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Mobile Lipid Bilayer on Agar-Coated Electrode Toward modeling cellular communication via gap junction Reviewed
Kenta Shimba, Yoshitaka Miyamoto, Tohru Yagi
2016 9TH BIOMEDICAL ENGINEERING INTERNATIONAL CONFERENCE (BMEICON) 2016
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Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device. Reviewed International journal
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
Cell medicine 8 ( 1-2 ) 47 - 56 2015.12
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Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device. Reviewed International journal
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
Cell medicine 7 ( 2 ) 67 - 74 2015.2
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Permeability of tubular membrane protein Reviewed
Atsushi Hattori, Tohru Yagi, Yoshitaka Miyamoto
BMEiCON 2013 - 6th Biomedical Engineering International Conference 2013
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Saccade-related EEG signals by ICA algorithms Reviewed
Arao Funase, Motoaki Mouri, Yagi Tohru, Andrzej Cichocki, Ichi Takumi
2008 INTERNATIONAL SYMPOSIUM ON INFORMATION THEORY AND ITS APPLICATIONS, VOLS 1-3 961 - + 2008
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Study on Mental Stress Using Near-Infrared Spectroscopy, Electroencephalography, and Peripheral Arterial Tonometry Reviewed
Yoshikazu Ishii, Hajime Ogata, Hidenori Takano, Hidenori Ohnishi, Toshiharu Mukai, Tohru Yagi
2008 30TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-8 2008 4992 - + 2008
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A study on the primary motor cortex performing motor imagery task for the development of a brain-computer interface Reviewed
Yoshikazu Ishii, Toshiharu Mukai, Tohru Yagi
2007 IEEE INTERNATIONAL CONFERENCE ON SYSTEMS, MAN AND CYBERNETICS, VOLS 1-8 3393 - + 2007
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Research for estimating direction of saccadic eye movements by single trial processing
Arao Funase, Tetsuro Hashimoto, Tohru Yagi, Allan K. Barros, Andrzej Cichocki, Ichi Takumi
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings 4723 - 4726 2007
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Single trial analysis on saccade-related EEG signal
Arao Funase, Tohru Yagi, Allan K. Barros, Andrzej Cichocki, Ichi Takumi
Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering 371 - 374 2007
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Drifting and blinking compensation in electro-oculography (EOG) eye-gaze interface
Tohru Yagi, Yoshiaki Kuno, Kazuo Koga, Toshiharu Mukai
Conference Proceedings - IEEE International Conference on Systems, Man and Cybernetics 4 3222 - 3226 2006
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Single trial method for brain-computer interface
Arao Funase, Tohru Yagi, Allan K. Barros, Andrzej Cichocki, Ichi Takumi
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings 5277 - 5281 2006
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Comparison of saccade-related EEG signal with saccade-related independent component
Arao Funase, Tohru Yagi, Allan K. Barros, Andrzej Cichocki, Ichi Takumi
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings 7 7060 - 7063 2005
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Biohybrid retinal implant: Research and development update in 2005
Tohru Yagi, Masami Watanabe, Yasushi Ohnishi, Shigeru Okuma, Toshiharu Mukai
2nd International IEEE EMBS Conference on Neural Engineering 2005 248 - 251 2005
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Fundamental research for EEG interface: EEG and auditory/visual stimulus during eye-movements
Arao Funase, Tohru Yagi, Yoshiaki Kuno, Yoshiki Uchikawa
Proceedings of the IEEE International Conference on Systems, Man and Cybernetics 2 1999
Books
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ブレイン-マシン・インタフェース最前線
株式会社 工業調査会 2007
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光技術動向調査報告書XIX
財団法人光産業技術振興協会 2003
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マイクロマシン
産業技術サービスセンター 2002
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Intelligent Robots and Systems
Elsevier 1995
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Intelligent Robots and Systems
Elsevier 1995
MISC
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A study on regular hexagonal culture substrates for separation of cells
佐藤颯, 下村裕哉, 菅野翔一朗, 榛葉健太, 宮本義孝, 八木透
電気学会研究会資料(Web) ( MBE-24-001-026 ) 2024
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Preparation of iris muscle-like tissue using mesh cell sheets
岡安悠杜, 榛葉健太, 宮本義孝, 八木透
電気学会研究会資料(Web) ( MBE-24-001-026 ) 2024
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A study on the change of shrinkage by culture days about cultured muscle actuators
豊浦達貴, 岡安悠杜, 下村裕哉, 菅野翔一朗, 榛葉健太, 宮本義孝, 八木透
電気学会研究会資料(Web) ( MBE-24-001-026 ) 2024
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Research on culture substrates for cell position control
下村裕哉, 佐藤颯, 菅野翔一朗, 榛葉健太, 宮本義孝, 八木透
電気学会研究会資料(Web) ( MBE-24-001-026 ) 2024
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Creation of sphincter muscle tissue by controlling myofiber orientation using pillars
岡安悠杜, 下村裕哉, 榛葉健太, 宮本義孝, 八木透
電気学会電子・情報・システム部門大会(Web) 2023 2023
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Development of a method for migration of adherent cells using magnetic particles
下村裕哉, 榛葉健太, 宮本義孝, 八木透
電気学会電子・情報・システム部門大会(Web) 2023 2023
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コンピュータ外科の新展開 次世代のキーテクノロジー 神経インタフェースの現状と将来
八木 透, 宮本 義孝, 菅原 路子, 中谷 裕教
日本コンピュータ外科学会誌 17 ( 3 ) 157 - 157 2015.10
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創造性教育のための電気回路実習
高橋秀智, 八木透
設計工学 Vol. ( No. ) 18,22 2007
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人工眼
八木透
細胞 37 ( 2 ) 18 - 21 2005
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Transretinal electrical stimulation with a suprachoroidal multichannel electrode in rabbit eyes
Hirokazu Sakaguchi, Takashi Fujikado, Xiaoyun Fang, Hiroyuki Kanda, Makoto Osanai, Kazuaki Nakauchi, Yasushi Ikuno, Motohiro Kamei, Tohru Yagi, Shigeru Nishimura, Masahito Ohji, Tetsuya Yagi, Yasuo Tano
Japanese Journal of Ophthalmology 48 ( 3 ) 256 - 261 2004.5
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ハイブリッド型人工網膜
八木透
応用物理 73 ( 8 ) 1095 - 1100 2004
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八木透
BME 18 ( 4 ) 36 - 42 2004
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人工網膜による機能再建
八木透
月刊眼科診療プラクティス 91 74 - 77 2003
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人工眼（人工視覚）
八木透
現代医療の最前線（後編）：人工臓器とメディカル･エンジニアリングの進歩 42 - 51 2003
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人工網膜とその情報処理
八木透
脳型コンピュータの実現に向けて:脳を知り、脳を創る 78 - 73 2003
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失明を克服するテクノロジー：人工眼
八木透
眼薬理 17 5 - 11 2003
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人工視覚研究の現状-工学的立場から
八木透
脳21 6 ( 4 ) 81 - 84 2003
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Analysis of EEG Related Saccadic Eye Movement
Shigeru Okuma, Arao Funase, Yoshiaki Kuno, Tohru Yagi
IEEJ Transactions on Electronics, Information and Systems 123 ( 1 ) 93 - 99 2003
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衝動性眼球運動（サッケード）関連脳波に対する独立成分解析
船瀬新王, AllanK.Barros, 大熊繁, AndrejCichocki 八木透
生体医工学 41 ( 4 ) 342 - 351 2003
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導電性高分子のマイクロパターン作製
伊藤雄一郎, 大西保志, 木内一壽, 八木透
電気学会論文誌 122-C ( 9 ) 1441 - 1446 2002
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八木透
画像ラボ 13 ( 7 ) 32 - 35 2002
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仮想的に再現された人工眼視覚のもとでの読書能力の定量的評価
寺澤靖雄, 八木
電気学会論文誌 122-C ( 7 ) 1104 - 1109 2002
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Analysis on saccade-related EEG with independent component analysis
A Funase, T Yagi, AK Barros, Y Kuno, Y Uchikawa
INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS AND MECHANICS 14 ( 1-4 ) 353 - 358 2001
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A study on conductive polymer electrodes for stimulating the nervous system
Y Ito, T Yagi, Y Ohnishi, K Kiuchi, Y Uchikawa
INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS AND MECHANICS 14 ( 1-4 ) 347 - 352 2001
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Effect of pulse parameters on visual nervous system
H Kanda, M Watanabe, T Fujikado, T Yagi
INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS AND MECHANICS 14 ( 1-4 ) 337 - 340 2001
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Simulation of visual prosthesis in virtual space
Y Terasawa, T Fujikado, T Yagi
INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS AND MECHANICS 15 ( 1-4 ) 431 - 436 2001
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Toward visual prosthesis: Electrical stimulation on the central nervous system
H Kanda, T Yagi, Y Ito, S Tanaka, M Watanabe, Y Uchikawa
NON-LINEAR ELECTROMAGNETIC SYSTEMS - ISEM '99 713 - 716 2000
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System for position detection and communication with image sensor
Y Kuno, T Yagi, S Suzuki, Y Uchikawa
NON-LINEAR ELECTROMAGNETIC SYSTEMS - ISEM '99 695 - 698 2000
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A study on Electro-Encephalogram (EEG) in eye movements
A Funase, T Yagi, Y Kuno, Y Uchikawa
NON-LINEAR ELECTROMAGNETIC SYSTEMS - ISEM '99 709 - 712 2000
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Distinct mechanisms for expression of Fos-like immunoreactivity and synaptic potentiation in telencephalic hyperstriatum of the quail chick
S. Yanagihara, T. Yagi, T. Matsushima
Brain Research 779 ( 1-2 ) 240 - 253 1998.1
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Distinct mechanisms for expression of Fos-like immunoreactivity and synaptic potentiation in telencephalic hyperstriatum of the quail chick
S Yanagihara, T Yagi, T Matsushima
BRAIN RESEARCH 779 ( 1-2 ) 240 - 253 1998.1
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EOGを用いた視線入力インタフェースの開発
久野悦章, 藤井一幸, 八木透, 内川嘉樹
情報処理学会論文誌 39 ( 5 ) 1455 - 1462 1998
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UCHIKAWA Yoshiki, YAGI Tohru
Journal of the Japanese Society for Artificial Organs and Tissues 27 ( 5 ) 703 - 707 1998
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Active vision inspired by mammalian fixation mechanism
Yagi, T. Asano, N. Makita, S. Uchikawa
Intelligent Robots and Systems 39 - 47 1995
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Active vision inspired by mammalian fixation mechanism
Yagi, T. Asano, N. Makita, S. Uchikawa
Intelligent Robots and Systems 39 - 47 1995
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Active Vision System Inspired by Biological Fixation Mechanism
YAGI Tohru, ASANO Nobuhisa, MAKITA Shinji, UCHIKAWA Yoshiki
Transactions of the Japan Society of Mechanical Engineers. Series C. 61 ( 591 ) 4402 - 4409 1995
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An algorithm of eye movements in biological vision, Simulation and Design of Applied Electromagnetic Systems
Yagi, T. Gouhara, K. Uchikawa
Elsevier Studies in Applied Electromagnetics in Materials 5 415 - 418 1993
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An algorithm of eye movements in biological vision, Simulation and Design of Applied Electromagnetic Systems
Yagi, T. Gouhara, K. Uchikawa
Elsevier Studies in Applied Electromagnetics in Materials 5 415 - 418 1993
Presentations
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Biohybrid retinal implant: Research and development update in 2005
2005
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Biohybrid retinal implant: Research and development update in 2005
2005
Awards
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計測自動制御学会SI部門奨励賞
2005
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計測自動制御学会システムインテグレーション部門SI2004 ベストセッション講演賞
2004
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平成16年電気関係学会東海支部連合大会奨励賞
2004
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平成10年度電気通信普及財団賞
1998
Research Projects
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Research and development of neural interface devices using nanotubes and artificial cell membranes
Grant number：23K25191 2022.4 - 2027.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）
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Research and development of neural interface devices using nanotubes and artificial cell membranes
Grant number：22H03937 2022.4 - 2027.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Grant amount：\17420000 （ Direct Cost: \13400000 、 Indirect Cost：\4020000 ）
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Neural interface for biohybrid artificial limbs that realize sensory and motor functions
Grant number：21H00323 2021.4 - 2023.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Grant amount：\11310000 （ Direct Cost: \8700000 、 Indirect Cost：\2610000 ）
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Research and development of neural interface device composed of artificial cell membrane and membrane protein
Grant number：18H03512 2018.4 - 2023.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Grant amount：\17160000 （ Direct Cost: \13200000 、 Indirect Cost：\3960000 ）
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The visualization of microtissues using near-infrared fluorescence endoscope
Grant number：16K12921 2016.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Miyamoto Yoshitaka, CHIBA Toshio, IKEUCHI Masashi, NOGUCHI Hirofumi, SHIMBA Kenta, SUZUKI Satoshi, HAYASHI Shuji
Grant amount：\3510000 （ Direct Cost: \2700000 、 Indirect Cost：\810000 ）
Near-infrared fluorescence endoscope system with indocyanine green (ICG) is a powerful tool to observe the interior tissues and organs in surgery in real time. It is useful especially in a wide range of surgical fields. Since ICG has strong near-infrared fluorescence, it can be visualized while excluding the effect of autofluorescence of tissues. In this study, we successfully labeled microtissues with ICG and then visualized them in real time. The near-infrared fluorescence endoscope system was helpful to observe microtissues with different sizes at high sensitivity (about 100-10,000 cell clusters) in vitro (the surface of pig small intestine, etc). Although the noise was amplified with increasing the sensitivity, it can be reduced by adjusting the threshold level. Our system can contribute to improving the safety of transplantation in regenerative therapy and cell medicine, and its medical applications will be expanded more and more in the future.
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Development of cryopreservation system for cells and tissue constructs in regenerative medicine and transplantation
Grant number：15H03039 2015.4 - 2018.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
MIYAMOTO Yoshitaka, MIYADO Kenji, YAGI Tohru, TERAMOTO Naozumi, NOGUCHI Hirofumi, IKUTA Koji, MIURA Takumi
Grant amount：\17030000 （ Direct Cost: \13100000 、 Indirect Cost：\3930000 ）
In this study, we developed a novel (1) DMSO-free cryopreservation solution, (2) culture device for cryopreservation, and (3) freezing system. (1) In order to safely freeze cells, it is necessary to solve the problems such as the cytotoxicity of DMSO, the antigenicity change of animal-derived materials and the possibility of contamination with pathogens. We found an optimal composition of cryopreservation solution without DMSO and serum for human cells. (2) 3D culture device TASCL was used to create a large amount of human tissue-derived stem cell constructs having a uniform size. In addition, we successfully determined a vitrified freezing condition based on the effective composition of the freezing solution. (3) In order to control cell freezing, we succeeded in developing a freezing system with a stirling engine and in optimizing freezing conditions. Consequently, a cryopreservation system of cell tissue construct for regeneration and transplantation medical care was established.
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Construction of a cytotoxicity and function evaluation system using combinatorial cell clusters with magnetic nanoparticles
Grant number：25560248 2013.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
MIYAMOTO Yoshitaka, IKEUCHI Masashi, NOGUCHI Hirofumi, YAGI Tohru, HAYASHI Shuji, SUZUKI Satoshi, IKUTA Koji
Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）
This study aims to construct an evaluation system by combinatorial cell clusters. The cell clusters with various sizes were successfully prepared efficiently on our developed micro-fabricated device in a one-cell seeding. Furthermore, commercially available MRI contrast agents and our magnetic nanoparticles for MRI imaging were assessed for toxicity and cell functions by combinatorial hepatocyte clusters formed on this device. As a result, resultant clusters were also successfully evaluated for cell viability, cell proliferation, cytotoxicity, and functions at the protein level. Furthermore, it was possible to confirm the reproducibility and efficacy of other cell types such as adipose-derived stem cells on their toxicity and functions.
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Development of Bio-stimulation Electrode Using Membrane Protein
Grant number：22650103 2010 - 2012
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
YAGI Tohru
Grant amount：\3440000 （ Direct Cost: \2900000 、 Indirect Cost：\540000 ）
Tubular membrane protein’s ion permeation function is available for biomedical applications such as cell stimulation devices. However, when considering that those proteins stimulate the multiple cells in parallel, it is not easy to evaluate each function to the cell at the same time by electrical measurements. Therefore, we propose the use of ion sensor to evaluate the protein’s electrical properties on the basis of its fluorescence images. In this study, we introduced target proteins into an artificial lipid bilayer membrane where the ion concentration gradient exists. Then, we observe fluorescence images and record electrical properties of the membrane.
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Construction of nerve cell body on a 3-dimensional culture device
Grant number：22650127 2010 - 2011
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
MIYAMOTO Yoshitaka, IKEUCHI Masashi, KAJI Noritada, YAGI Tohru, HAYASHI Shuji
Grant amount：\3320000 （ Direct Cost: \2900000 、 Indirect Cost：\420000 ）
This study aims to develop a device for the construction of three dimensional cell assemblies of nerve-like cells. The device for cell patterning has enabled to generate cell assemblies with a controlled size in large numbers and to simultaneously evaluate their functions under various culture conditions. The size of the tissues was controlled for primary cells, tissue-derived stem cells, and induced plulipotent stem(iPS) cells. The cell assemblies on the device had their drug response, and protein level analysis revealed their some functions. The 2D culture of nerve-like cells also showed that a neural-like network was generated on the device and cyclic response to electric nerve stimulation was changed.
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Development of an Artificial Synapse using an Integrated Electrochemical Micropump
Grant number：18300154 2006 - 2009
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
YOSHIMI Yasuo, KANAMORI Toshiyuki, YAGI Tohru
Grant amount：\13300000 （ Direct Cost: \11800000 、 Indirect Cost：\1500000 ）
The final purpose of this project is a development of integrated micropumps working as an interface between electric circuits and neurons or an artificial synapse. We developed a chip of micropump with microvalve using electrodecomposition of water as a driving force. The chip can control the jetting with punctuality to the second. The pump could induce excitatory postsynaptic potential of Aplysia neuron by neurotransmitter administration. We developed also voltage imaging for central nerve system for evaluation of the ability of the interface. It has been difficult to detect the action potential of Aplysia neuron by voltage imaging. We succeeded in the detection of the action potential by prolonging by tetraethylammonium chloride as a potassium channel blocker. The technologies developed in the project will help the development of neuronal interface by neurotransmitter administration.
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Visual Prosthesis -Study of hybrid retinal implant-
Grant number：12358016 2000 - 2003
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
MIYAKE Yuzo, KONDO Mineko, WATANABE Masami, ISHIGURO Akio, KONDO Nagako
Grant amount：\47730000 （ Direct Cost: \39600000 、 Indirect Cost：\8130000 ）
(1) Simulation of visual prosthesis in virtual system : It is essential to determine the specifications which meet the minimal needs in daily lives of patients. For this purpose, a prosthetic vision simulator was developed, which enables to experience prosthetic vision in the visual space. Experimental results suggested that the electric current intensity in simulation has small effects to the reading ability. (2) Imaging signal analysis : To get proper electrical stimulation to the retina, the special hardware was developed with a compact curcuit. We proved this device is essential for retinal prosthesis. (3) Evaluation of electrical stimulus to visual neuron : To investigate the most suitable stimulus condition of the electrical pulse to the retina, normal and retinal degenerated rats were used. We found that short wave stimuli are more effective than pulse waves. Using cats, the visual evoked response from the visual cortex were evaluated changing the pulse width, pulse intensity and distance of electrodes by putting the stimulus electrode array on the lateral geniculate. We found that the pulse intensity influences the response more than pulse width. (4) The modification of stimulus electrode for human retina : Several modifications were made for the retinal exoplant in human. The optical coherence tomography was found to be a good monitor to check the depth and location of the exoplant. (5) Survival and axonal regeneration of retinal ganglion cells in adult cats : We introduced several methods to rescure the axotomized cat retinal ganglion cells from apotosis and regulate their axons ; transplantation of the peripheral nerve, intraocular injections of neurotrophic factors, or an antiapotopic drug.
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導電性高分子を用いたバイオ・シリコン融合素子に関する研究
Grant number：12838006 2000 - 2002
日本学術振興会 科学研究費助成事業 基盤研究(C)
内川 嘉樹, 大西 保志, 八木 透
Grant amount：\2300000 （ Direct Cost: \2300000 ）
半導体素子上に培養した神経細胞回路において問題となるのは、神経細胞と素子との親和性である。そこで本研究では、神経細胞と電極をつなぐインタフェースとして導電性高分子を使用し、生体との親和性を高めつつ、素子から神経細胞への信号伝達効率を下げないような、バイオ/シリコン融合素子の開発を目指す。導電性高分子は生体との親和性も高く、かつ導電性を持つことが特徴であるが、その加工方法には工夫を要する。導電性高分子の多くは、炭素-炭素共役二重結合がつながった分子構造をしているため、不融不溶であり、重合後に通常の高分子の熱加工方法などは適用できないと言う欠点を持っている。そこで本年度は、ポリビニルアルコールをマトリックスとする方法により、ポリピロールのマイクロパターン作製と、培養細胞を用いた生体適合性の評価を行なった。パターン作製には、酸化重合剤の光反応性を利用したフォトリソグラフィを用いた。その結果、マスクパターンの露光時間やピロールの重合時間を最適に設定すれば、最小線幅3μmのポリピロールパターンを作製できることが判明した。またポリピロール上に細胞を培養した結果、培養細胞は突起を伸展して成長し、4週間にわたって生存した。これらの結果より、電極アレイに適用可能な微小サイズまでポリピロールパターンを微小化できることが示された。またポリピロールは高い生体適合性を持つことが示された。
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半導体素子上に培養した神経細胞の回路形成に関する研究
Grant number：12019229 1999 - 2000
日本学術振興会 科学研究費助成事業 特定領域研究(A)
八木 透, 時田 義人, 児玉 哲司
Grant amount：\1900000 （ Direct Cost: \1900000 ）
半導体素子上に神経細胞を培養し、その軸索伸長を人工的に制御して神経回路を形成させる技術について研究を行う。そして将来は「神経インタフェース」や「バイオチッブ」の開発へと発展させる計画である。これまでITO(indium tin oxide)や白金など、生体適合性が比較的高いといわれる金属材料を用いて作製した微小電極アレイ上に神経細胞を培養し、その軸索誘導を試みてきた。しかし電極アレイ上に細胞を直接培養しようとすると、細胞が培養途中で死滅してしまうため、ポリリジンや細胞外マトリクス分子で電極アレイ表面をコーティングする必要があった。しかしコーティングはインピーダンスを増加させるため、細胞への電気刺激ならびに細胞からの電気記録には好ましくない。したがって生体組織との適合性および導電性の低下を同時に解決する必要がある。そこで我々は、導電性高分子の一つであるポリピロールを材料とすることにより、高い生体適合性と導電性を持つ電極アレイが作製できると考えた。
そこで本年度は、ポリピロールのマイクロパターン作製と、培養細胞を用いた生体適合性の評価を行なった。パターン作製には、酸化重合剤の光反応性を利用したフォトリソグラフィを用いた。その結果、マスクパターンの露光時間やピロールの重合時間を最適に設定すれば、最小線幅3μmのポリピロールパターンを作製できることが判明した。またポリピロール上に細胞を培養した結果、培養細胞は突起を伸展して成長し4週間にわたって生存した。これらの結果より、電極アレイに適用可能な微小サイズまでポリピロールパターンを微小化できることが示された。またポリピロールは高い生体適合性を持つことが示された。 -
半導体素子上に培養した神経細胞の回路形成に関する研究
Grant number：11132229 1999
日本学術振興会 科学研究費助成事業 特定領域研究(A)
八木 透, 時田 義人, 児玉 哲司
Grant amount：\2000000 （ Direct Cost: \2000000 ）
神経回路を人工的に作る方法として、1)軸索伸長に関係する各種タンパク分子の濃度勾配を人工的に作る、2)生体組織に親和性の高い物質で基盤上にパターンを作る、3)電界をつくる、4)軸索が伸長しやすいような溝(ガイドウェイ)を培養ディッシュ底につくる、などが考えられる。これらのうち今回は3)に着目して、電界と軸索伸長について調べる。
まず、電極周辺にどのような電界が形成されるかについてコンピュータシミュレーションを行った。シミュレーションでは、正極と負極に1.5Vの電圧を加え、ポアソン方程式と有限要素法を用いて電界を計算している。なお電極周囲はリンガー液で満たされているものとした。図1は、電極周辺に形成される電界分布を色の濃淡で示したグラフで、左から正負電極間距離が100、200、300μmの場合を示している。同図の上段は電極を断面方向から眺めた場合の電界分布、下段は上方から眺めた場合の電界分布のうち長軸方向の半分の分布を表示している。容易に推測されるように、電極近傍で高い電界強度が観測されている。また正負極間の距離を変更すると電界分布が大きく変化することがわかる。また基盤内部に電界が形成されていることがわかるが、これはクロストークの原因になる可能性があり、さらには電極と神経組織との信号伝達効率を低下させる可能性があると考えられる。
次に、市販の多点電極ディッシュ(ガラス基盤上にフォトリソグラフィでITOを用いてパターン化し、ポリイミドでコーティングをしたもので、電極部分が金でめっきされている)の上に、成ラットの後根神経節から採取した神経細胞を培養し、任意の電極を正極と負極に選び、1.5Vの直流電圧を加えて形成した静電界において、神経細胞の軸索伸長を観察した。なお基盤上への神経細胞の接着を良くする目的で接着分子を塗布した(ポリリジン、コンカナバリン、コラーゲンの3種について実施)。負極に向けて神経突起の伸展が観察されるのではないかと予想したが、今回の実験条件ではそのような傾向は見られず、またコントロールとの比較においても特定方向への突起伸展は観察できなかった。今後は、電界条件を変更して突起伸長との関係を検討する。 -
神経細胞を用いた生体信号計測システム「神経プローブ」に関する研究
Grant number：11750367 1998 - 2000
日本学術振興会 科学研究費助成事業 奨励研究(A)
八木 透
Grant amount：\2200000 （ Direct Cost: \2200000 ）
半導体素子上に神経細胞を培養し、その軸索伸長を人工的に制御して神経回路を形成させる技術について研究を行う。これまでITO(indium tin oxide)や白金など、生体適合性が比較的高いといわれる金属材料を用いて作製した微小電極アレイ上に神経細胞を培養し、その軸索誘導を試みてきた。しかし電極アレイ上に細胞を直接培養しようとすると、細胞が培養途中で死滅してしまうため、ポリリジンや細胞外マトリクス分子で電極アレイ表面をコーティングする必要があった。しかしコーティングはインピーダンスを増加させるため、細胞への電気刺激ならびに細胞からの電気記録には好ましくない。したがって生体組織との適合性および導電性の低下を同時に解決する必要がある。そこで、導電性高分子の一つであるポリピロールを材料とすることにより、高い生体適合性と導電性を持つ電極アレイが作製できると考え、ポリピロールのマイクロパターン作製と、培養細胞を用いた生体適合性の評価を行なった。パターン作製には、酸化重合剤の光反応性を利用したフォトリソグラフィを用いた。その結果、マスクパターンの露光時間やピロールの重合時間を最適に設定すれば、最小線幅3μmのポリピロールパターンを作製できることが判明した。またポリピロール上に細胞を培養した結果、培養細胞は突起を伸展して成長し、4週間にわたって生存した。
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培養細胞と半導体素子を用いたハイブリット型人工網膜に関する研究
Grant number：10145105 1998
日本学術振興会 科学研究費助成事業 特定領域研究(A)
内川 嘉樹, 八木 透, 時田 義人, 児玉 哲司, 川瀬 和秀, 渡部 眞三
Grant amount：\2000000 （ Direct Cost: \2000000 ）
本研究では、培養神経細胞と光電素子を中心としたMEMS(Micro-Electro-MechanicalSystem)を組み合わせ、光覚回復を目的とした人工眼「ハイブリッド型人工網膜」の研究開発を行なっている。外部装置から光通信で送られたデータに基づき、埋め込んだ培養神経細胞を電気的に刺激する。なお末梢神経を用いて神経細胞と中枢の間を架橋することにより、その軸索を中枢へ導き、信号を伝達させようと考えている。従って提案する人工眼では、電極から培養神経細胞へ、さらに培養神経細胞から視覚中枢への伝達の安定的な再構成が、本質的な課題である。本年度は、「神経インターフェースの生体適合性と擬似神経パルスの評価」と、「外部装置から神経インターフェースへのデータ転送」について研究を行なった。
1. 神経インターフェースの生体適合性と擬似神経パルスの評価
動物の脳への電気刺激により、視覚野で生じる視覚誘発電位を測定した。まずは単一パルスに対する応答を記録した。パルス幅を数百μ秒から数ミリ秒まで、パルスの大きさを数μアンペアから数百μアンペアまで変化させ、電気刺激を行なったところ、パルス幅の変化よりもパルスの大きさの変化に視覚野の応答が影響されることが判明した。
2. 外部装置から神経インターフェースへのデータ転送
提案する人工網膜では、体外で取得した画像データ(アナログ信号)をデジタル信号へ符号化した後、光通信方式で神経インターフェースへ伝送し、神経インターフェース内の回路で擬似神経パルスを生成する必要がある。そこで赤外線LED、フォトダイオード、A/Dコンバータ、D/Aコンバータを用いて電子回路を作製し、通信の実験を行なった。その結果、比較的単純な回路でも十分にデータ転送が行なえることが判明した。 -
A Study on Implantable Retinal Implant using Cell Culture Technique
Grant number：08558097 1996 - 1998
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
UCHIKAWA Yoshiki, WATANABE Masami, YAGI Tohru, KODAMA Tetsuji, KAWASE Kazuhide
Grant amount：\16600000 （ Direct Cost: \16600000 ）
A hybrid retinal implant is a visual prosthesis to reconstruct visual sensation in the blind. It will consist of an outer apparatus and an implanted MBMS (micro-electro mechanical systems). In order to connect the MEMS with the CNS (central nervous systems) , several neurons will be cultured on the top of the MEMS.Then, their axons will be guided with the peripheral nerve fiber. After the axons reach towards the CNS, they will make the proper synaptic connection with the CNS neurons. Therefore, the adequate electrical stimulation will lead the neural cells to produce the action potential so that functional connection will be realized.
The visual prosthesis "Hybrid Retinal Implant requires to facilitate a micro-electrode array for extracellular stimulation of nervous systems. This time, using micro-machine technology, we have fabricated a prototype of the array. In this array, aluminum cable leads are sandwiched with two dielectric layers ; silicon dioxyside and silicon nitride. First, a 400 mum thickness silicon wafer was oxidized to a thickness of 1 mum. Conductors of aluminum were next deposited to a thickness of 0.3 mum and patterned. Then, the upper dielectric layer (i.e. silicon nitride) was deposited. Using a reactive ion etching process, silicon nitride layer was partially exposed to make electrode sites. This array consists of nine channel bipolar electrodes. Two adjacent electrodes are facing each other to make nine anode/cathode pairs in a 3x3 lattice. Each electrode has 20x20 mum square shape. The distance of each pair is up to 320 mum ; therefore, the cross-talk between each pair will be reduced. Our previous computational analysis have revealed that this specification of the micro-electrode array leads to the efficient electrical stimulation to neurons. In order to evaluate its real performance, in-vivo/in-vitro extracellular stimulation experiments will be performed in our next study.
For more information on this research, you may contact "http : //www. cmplx. cse. nagoya-u. ac. jp/research/retina/".