Organization
School of Science Associate Professor
Title
Associate Professor
External link
MATSUSHITA MICHIO
News & Topics
-
Pinpointing biomolecules with nanometer accuracy
2019/10/21
Languages： English
Scientists at Tokyo Institute of Technology (Tokyo Tech) demonstrate a new approach in fluorescence microscopy that can be used to locate individual biomolecules in 3D space with nanometer-scale p
-
クライオ蛍光顕微鏡で分子イメージングに成功 鍵はナノレベルのピント合わせにあり
2019/10/11
Languages： Japanese
要点-ナノメートル正確度のクライオ蛍光顕微鏡が完成-通常の蛍光顕微鏡に比べて2桁高い正確度-長さ10ナノメートルの二本鎖DNAの両末端を1分子ごとに可視化概要東京工業大学理学院物理学系の古林琢大学院生、石田啓太大学院生、松下道雄准教授、藤
-
2019/07/17
Languages： Japanese
極限環境への耐性、広視野、高開口数、すべての収差の補正を並立 成功の鍵は、対物レンズ設計では非主流の反射光学系 生命現象の光イメージングに道筋をつける新技術
-
世界初、光学顕微鏡で三次元分子解像度を実現 ―生命現象の分子レベル画像化に期待―
2017/07/04
Languages： Japanese
一つ一つの生体分子の三次元位置をそのサイズと同等の解像度で観察可能に 手作り光学顕微鏡だからできた世界最高の解像度 鮮明な画像の鍵は超流動ヘリウムとその中で使える反射対物レンズ
Degree
-
Doctor of Science ( Kyoto University )
-
Master of Science ( Kyoto University )
Research Areas
-
Life Science / Biophysics
-
Natural Science / Semiconductors, optical properties of condensed matter and atomic physics
Education
-
京都大学大学院 理学研究科 化学専攻
- 1992
Country： Japan
-
Kyoto University Graduate School, Division of Natural Science
- 1992
-
Kyoto University Faculty of Science
- 1987
Country： Japan
Research History
-
-:東京工業大学 大学院理工学研究科 助教授
2002
-
-:Tokyo Institute of Technology Department of Physics Associate Professor
2002
-
:オランダ・ライデン大学 博士研究員
2000 - 2002
-
:Leiden University, The Netherlands Post-doctoral Fellow
2000 - 2002
-
:分子科学研究所 助手
1993 - 2000
-
:Institute for Molecular Science Research Associate
1993 - 2000
Professional Memberships
-
The Spectroscopical Society of Japan
-
日本生物理学会
-
The Chemical Society of Japan
-
The Physical Society of Japan
-
日本分光学会
-
日本化学会
-
日本物理学会
-
The Biophysical Society of Japan
Papers
-
Single-Molecule 3D Nanoscopy for Application to Cryofixed Samples
Kanta Naruse, Tsuyoshi Matsuda, Yuta Mizouchi, Namiki Tominaga, Kento Takishima, Naoki Kamiya, Eiji Nakata, Takashi Morii, Michio Matsushita, Satoru Fujiyoshi
The Journal of Physical Chemistry B 2025.10
-
Kohei Otomo, Takehisa Dewa, Michio Matsushita, Satoru Fujiyoshi
The Journal of Physical Chemistry B 2023.5
-
Erratum: Superfluid helium nanoscope insert with millimeter working range (Rev. Sci. Instrum. (2022) 93 (103703) DOI: 10.1063/5.0107395)
Naoki Kamiya, Kazuki Kuramoto, Kento Takishima, Tatsuya Yumoto, Haruka Oda, Takeshi Shimi, Hiroshi Kimura, Michio Matsushita, Satoru Fujiyoshi
Review of Scientific Instruments 93 ( 12 ) 2022.12
-
Superfluid helium nanoscope insert with millimeter working range Reviewed
Naoki Kamiya, Kazuki Kuramoto, Kento Takishima, Tatsuya Yumoto, Haruka Oda, Takeshi Shimi, Hiroshi Kimura, Michio Matsushita, Satoru Fujiyoshi
Review of Scientific Instruments 93 ( 10 ) 103703 - 103703 2022.10
-
Toru Kondo, Risa Mutoh, Shun Arai, Genji Kurisu, Hirozo Oh-oka, Satoru Fujiyoshi, Michio Matsushita
The Journal of Chemical Physics 156 ( 10 ) 105102 - 105102 2022.3
-
Variable immersion microscopy with a high numerical aperture
Keita Ishida, Kanta Naruse, Yuta Mizouchi, Yoshihiro Ogawa, Michio Matsushita, Takeshi Shimi, Hiroshi Kimura, Satoru Fujiyoshi
OPTICS LETTERS 46 ( 4 ) 856 - 859 2021.2
-
Cryogenic Far-Field Fluorescence Nanoscopy: Evaluation with DNA Origami Reviewed
Taku Furubayashi, Keita Ishida, Eiji Nakata, Takashi Morii, Kanta Naruse, Michio Matsushita, Satoru Fujiyoshi
JOURNAL OF PHYSICAL CHEMISTRY B 124 ( 35 ) 7525 - 7536 2020.9
-
Cryogenic Single-Molecule Spectroscopy of the Primary Electron Acceptor in the Photosynthetic Reaction Center Reviewed International journal
Toru Kondo, Risa Mutoh, Hiroaki Tabe, Genji Kurisu, Hirozo Oh-Oka, Satoru Fujiyoshi, Michio Matsushita
JOURNAL OF PHYSICAL CHEMISTRY LETTERS 11 ( 10 ) 3980 - 3986 2020.5
-
Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules Reviewed
Taku Furubayashi, Keita Ishida, Hiromu Kashida, Eiji Nakata, Takashi Morii, Michio Matsushita, Satoru Fujiyoshi
JOURNAL OF PHYSICAL CHEMISTRY LETTERS 10 ( 19 ) 5841 - 5846 2019.10
-
Aberration-corrected cryogenic objective mirror with a 0.93 numerical aperture Reviewed
Masanori Fujiwara, Takaki Ishii, Keita Ishida, Yasuharu Toratani, Taku Furubayashi, Michio Matsushita, Satoru Fujiyoshi
APPLIED PHYSICS LETTERS 115 ( 3 ) 2019.7
-
Tabe Hiroaki, Sukenobe Kei, Kondo Toru, Sakurai Atsunori, Maruo Minako, Shimauchi Akari, Hirano Mitsuharu, Uno Shin-nosuke, Kamiya MakoYYY, Urano Yasuteru, Matsushita Michio, Fujiyoshi Satoru
JOURNAL OF PHYSICAL CHEMISTRY B 122 ( 27 ) 6906 - 6911 2018.7
-
Three-Dimensional Localization of an Individual Fluorescent Molecule with Angstrom Precision Reviewed
Taku Furubayashi, Kazuya Motohashi, Keisuke Wakao, Tsuyoshi Matsuda, Isao Kii, Takamitsu Hosoya, Nobuhiro Hayashi, Mahito Sadaie, Fuyuki Ishikawa, Michio Matsushita, Satoru Fujiyoshi
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139 ( 26 ) 8990 - 8994 2017.7
-
Reflecting microscope system with a 0.99 numerical aperture designed for three-dimensional fluorescence imaging of individual molecules at cryogenic temperatures Reviewed
H. Inagawa, Y. Toratani, K. Motohashi, I. Nakamura, M. Matsushita, S. Fujiyoshi
SCIENTIFIC REPORTS 5 2015.8
-
Temperature-cycle microscopy reveals single-molecule conformational heterogeneity Reviewed
Haifeng Yuan, Alexander Gaiduk, Joanna R. Siekierzycka, Satoru Fujiyoshi, Michio Matsushita, Daniel Nettels, Benjamin Schuler, Claus A. M. Seidel, Michel Orrit
PHYSICAL CHEMISTRY CHEMICAL PHYSICS 17 ( 9 ) 6532 - 6544 2015
-
Spectroscopy of single Pr3+ ion in LaF3 crystal at 1.5 K Reviewed
Ippei Nakamura, Tatsuya Yoshihiro, Hironori Inagawa, Satoru Fujiyoshi, Michio Matsushita
SCIENTIFIC REPORTS 4 7364 2014.12
-
Three-dimensional laser-scanning confocal reflecting microscope for multicolor single-molecule imaging at 1.5 K Reviewed
Minako Maruo, Hironori Inagawa, Yasuharu Toratani, Toru Kondo, Michio Matsushita, Satoru Fujiyoshi
CHEMICAL PHYSICS LETTERS 591 233 - 236 2014.1
-
Kondo Toru, Mutoh Risa, Kurisu Genji, Oh-oka Hirozo, Fujiyoshi Satoru, Matsushita Michio
Seibutsu Butsuri 54 ( 1 ) S182 2014
-
Mechanical stability of a microscope setup working at a few kelvins for single-molecule localization Reviewed
Takuya Hinohara, Yuki I. Hamada, Ippei Nakamura, Michio Matsushita, Satoru Fujiyoshi
CHEMICAL PHYSICS 419 246 - 249 2013.6
-
3P252 Cryogenic single molecule spectroscopy of the electron transfer cofactor in the photosynthetic reaction center(18B. Photobiology: Photosynthesis,Poster)
Kondo Toru, Mutoh Risa, Kurisu Genji, Oh-oka Hirozo, Fujiyoshi Satoru, Matsushita Michio
Seibutsu Butsuri 53 ( 1 ) S253 2013
-
Single-protein study of photoresistance of pigment-protein complex in lipid bilayer Reviewed
Daisuke Uchiyama, Hajime Hoshino, Kohei Otomo, Taro Kato, Ken-ichi Onda, Akira Watanabe, Hiroyuki Oikawa, Satoru Fujiyoshi, Michio Matsushita, Mamoru Nango, Natsuko Watanabe, Ayumi Sumino, Takehisa Dewa
CHEMICAL PHYSICS LETTERS 511 ( 1-3 ) 135 - 137 2011.7
-
Structural Change of a Cofactor Binding Site of Flavoprotein Detected by Single-Protein Fluorescence Spectroscopy at 1.5 K Reviewed
Satoru Fujiyoshi, Mitsuharu Hirano, Michio Matsushita, Mineo Iseki, Masakatsu Watanabe
PHYSICAL REVIEW LETTERS 106 ( 7 ) 078101 2011.2
-
Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K Reviewed
Daisuke Uchiyama, Hiroyuki Oikawa, Kohei Otomo, Mamoru Nango, Takehisa Dewa, Satoru Fujiyoshi, Michio Matsushita
PHYSICAL CHEMISTRY CHEMICAL PHYSICS 13 ( 24 ) 11615 - 11619 2011
-
Single-component reflecting objective for low-temperature imaging and spectroscopy of single nano objects Reviewed
Masanori Fujiwara, Satoru Fujiyoshi, Michio Matsushita
SELECTED PAPERS FROM 17TH INTERNATIONAL CONFERENCE ON DYNAMICAL PROCESSES IN EXCITED STATES OF SOLIDS (DPC'10) 13 2011
-
Vibrational Microspectroscopy of Single Proteins Reviewed
Satoru Fujiyoshi, Yo Furuya, Mineo Iseki, Masakatsu Watanabe, Michio Matsushita
JOURNAL OF PHYSICAL CHEMISTRY LETTERS 1 ( 17 ) 2541 - 2545 2010.9
-
Single-component reflecting objective for ultraviolet imaging and spectroscopy at cryogenic temperature
Masanori Fujiwara, Satoru Fujiyoshi, Michio Matsushita
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS 26 ( 7 ) 1395 - 1399 2009.7
-
Visible fluorescence spectroscopy of single proteins at liquid-helium temperature (vol 100, artn 168101, 2008) Reviewed
Satoru Fujiyoshi, Masanori Fujiwara, Michio Matsushita
PHYSICAL REVIEW LETTERS 100 ( 17 ) 2008.5
-
Visible fluorescence spectroscopy of single proteins at liquid-helium temperature
Satoru Fujiyoshi, Masanori Fujiwara, Michio Matsushita
PHYSICAL REVIEW LETTERS 100 ( 16 ) 168101 2008.4
-
How deep is the potential well confining a protein in a specific conformation? A single-molecule study on temperature dependence of conformational change between 5 and 18 K
Hiroyuki Oikawa, Satoru Fujiyoshi, Takehisa Dewa, Mamoru Nango, Michio Matsushita
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130 ( 14 ) 4580 - + 2008.4
-
3P-268 A single-molecule study on conformational change of light-harvesting complex between 5 and 18K(The 46th Annual Meeting of the Biophysical Society of Japan)
Oikawa Hiroyuki, Fujiyoshi Satoru, Dewa Takehisa, Nango Mamoru, Matsushita Michio
Seibutsu Butsuri 48 S169 2008
-
Single-component reflecting objective for low-temperature spectroscopy in the entire visible region
Satoru Fujiyoshi, Masanori Fujiwara, Changman Kim, Michio Matsushita, Antoine M. van Oijen, Jan Schmidt
APPLIED PHYSICS LETTERS 91 ( 5 ) 051125-051125-3 2007.7
-
Probing the electronic structure and conformational flexibility of individual light-harvesting 3 complexes by optical single-molecule spectroscopy Reviewed
Martijn Ketelaars, Jean-Manuel Segura, Silke Oellerich, Ward P. F. de Ruijter, Gerhard Magis, Thijs J. Aartsma, Michio Matsushita, Jan Schmidt, Richard J. Cogdell, Juergen Koehler
JOURNAL OF PHYSICAL CHEMISTRY B 110 ( 37 ) 18710 - 18717 2006.9
-
Single-crystal EPR study at 95 GHz of the type 2 copper site of the inhibitor-bound quercetin 2,3-dioxygenase Reviewed
M Fittipaldi, RA Steiner, M Matsushita, BW Dijkstra, EJJ Groenen, M Huber
BIOPHYSICAL JOURNAL 85 ( 6 ) 4047 - 4054 2003.12
-
Single-molecule study of the electronic couplings in a circular array of molecules: Light-harvesting-2 complex from Rhodospirillum molischianum Reviewed
C Hofmann, M Ketelaars, M Matsushita, H Michel, TJ Aartsma, J Kohler
PHYSICAL REVIEW LETTERS 90 ( 1 ) 2003.1
-
Single-molecule study of the electronic couplings in a circular array of molecules: Light-harvesting-2 complex from Rhodospirillum molischianum
C Hofmann, M Ketelaars, M Matsushita, H Michel, TJ Aartsma, J Kohler
PHYSICAL REVIEW LETTERS 90 ( 1 ) 013004 2003.1
-
Single molecules as nanoprobes. A study of the Shpol'skii effect
M Matsushita, A Bloess, Y Durand, JYP Butter, J Schmidt, EJJ Groenen
JOURNAL OF CHEMICAL PHYSICS 117 ( 7 ) 3383 - 3390 2002.8
-
Optical far-field microscopy of single molecules with 3.4 nm lateral resolution
A Bloess, Y Durand, M Matsushita, H Van der Meer, GJ Brakenhoff, J Schmidt
JOURNAL OF MICROSCOPY-OXFORD 205 ( 1 ) 76 - 85 2002.1
-
Optical spectroscopy of individual photosynthetic pigment protein complexes
J Kohler, AM van Oijen, M Ketelaars, C Hofmann, M Matsushita, TJ Aartsma, J Schmidt
INTERNATIONAL JOURNAL OF MODERN PHYSICS B 15 ( 28-30 ) 3633 - 3636 2001.12
-
A single-molecule study of the relation between the resonance frequency and the orientation of a guest molecule in a Shpol'skii system
A Bloess, Y Durand, M Matsushita, J Schmidt, EJJ Groenen
CHEMICAL PHYSICS LETTERS 344 ( 1-2 ) 55 - 60 2001.8
-
Submicron structure of a Shpol'skii matrix: A single-molecule study of dibenzanthanthrene in n-tetradecane. Reviewed
A Bloess, Y Durand, M Matsushita, EJJ Groenen, J Schmidt
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 221 U291 - U291 2001.4
-
Microscopic structure in a Shpol'skii system: A single-molecule study of dibenzanthanthrene in n-tetradecane
A Bloess, Y Durand, M Matsushita, R Verberk, EJJ Groenen, J Schmidt
JOURNAL OF PHYSICAL CHEMISTRY A 105 ( 13 ) 3016 - 3021 2001.4
-
Spectroscopy on the B850 band of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila II. Exciton states of an elliptically deformed ring aggregate
M Matsushita, M Ketelaars, AM van Oijen, J Kohler, TJ Aartsma, J Schmidt
BIOPHYSICAL JOURNAL 80 ( 3 ) 1604 - 1614 2001.3
-
Spectroscopy on the B850 band of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila I. Experiments and Monte Carlo simulations Reviewed
M Ketelaars, AM van Oijen, M Matsushita, J Kohler, J Schmidt, TJ Aartsma
BIOPHYSICAL JOURNAL 80 ( 3 ) 1591 - 1603 2001.3
-
Spectroscopy on the B850 band of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila I. Experiments and Monte Carlo simulations
M Ketelaars, AM van Oijen, M Matsushita, J Kohler, J Schmidt, TJ Aartsma
BIOPHYSICAL JOURNAL 80 ( 3 ) 1591 - 1603 2001.3
-
Spectroscopy on the B850 band of individual light-harvesting 2 complexes of Rhodopseudomonas acidophila II. Exciton states of an elliptically deformed ring aggregate Reviewed
M Matsushita, M Ketelaars, AM van Oijen, J Kohler, TJ Aartsma, J Schmidt
BIOPHYSICAL JOURNAL 80 ( 3 ) 1604 - 1614 2001.3
-
Exciton states of an elliptically deformed LH2 ring. Reviewed
TJ Aartsma, M Matsushita, M Ketelaars, M Matushita, AM van Oijen, J Koehler, J Schmidt
BIOPHYSICAL JOURNAL 80 ( 1 ) 152A - 152A 2001.1
-
A Single Molecule Study of the Microscopic Structure in a Shpol'skii System
Michio Matsushita, A. Bloess, R. Verberk, Y. Dur, E.J.J, Groenen, J. Schmidt
2nd International Symposium on Physics, Chemistry and Biology with Single Molecules (Kloster Banz, Germany) 70 2001
-
Quasimicrowave spectroscopy of nonpolar diatomic molecules by using optical phase-locked lasers
M Kumagai, H Kanamori, M Matsushita, T Kato
JOURNAL OF CHEMICAL PHYSICS 113 ( 17 ) 7031 - 7034 2000.11
-
Spectroscopy of individual light-harvesting complexes
Matsushita M., Martijn Ketelaars, van Oijen Antoine M., Juergen Koehler, Thijs J.Aartsma, Jan Schmidt
Seibutsu Butsuri 40 S127 2000
-
Development of phase-lock system between two single-mode lasers for optical-optical double resonance spectroscopy
M Kumagai, H Kanamori, M Matsushita, T Kato
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS 38 ( 10 ) 6102 - 6106 1999.10
-
NQR by coherent Raman scattering of a triplet exciton in a molecular crystal
M Matsushita, T Kato
PHYSICAL REVIEW LETTERS 83 ( 10 ) 2018 - 2021 1999.9
-
Luminescence properties and crystal structures of dicyano(diimine)platinum(II) complexes controlled by Pt center dot center dot center dot Pt and pi-pi interactions
M Kato, C Kosuge, K Morii, JS Ahn, H Kitagawa, T Mitani, M Matsushita, T Kato, S Yano, M Kimura
INORGANIC CHEMISTRY 38 ( 7 ) 1638 - + 1999.4
-
Coherent Raman spectroscopy of nuclear quadrupole resonance of La around Pr3+ in LaF3 Reviewed
M Matsushita, A Mutoh, T Kato
PHYSICAL REVIEW B 58 ( 21 ) 14372 - 14382 1998.12
-
Coherent Raman spectroscopy of nuclear quadrupole resonance of La around Pr^3+^ in LaF_3_ Reviewed
Michio Matsushita, Akiko Mutoh, Tatsuhisa Kato
Phys. Rev. B 58 ( 21 ) 14372 - 14382 1998
-
Nuclear quadrupole resonance of La-139 around Pr3+ doped in LaF3. Raman heterodyne detection using optical transition of Pr3+ ions
M Matsushita, T Kato
CHEMICAL PHYSICS LETTERS 273 ( 3-4 ) 291 - 295 1997.7
-
Group theoretical study of the radical cation of methane: The effect of tunneling motions on the hyperfine interaction (vol 103, pg 3367, 1995) Reviewed
M Matsushita, T Momose, T Shida, LB Knight
JOURNAL OF CHEMICAL PHYSICS 104 ( 5 ) 2124 - 2124 1996.2
-
Dynamics of the three methionyl side chains of Streptomyces subtilisin inhibitor. Deuterium NMR studies in solution and in the solid state
A Tamura, M Matsushita, A Naito, S Kojima, KI Miura, K Akasaka
PROTEIN SCIENCE 5 ( 1 ) 127 - 139 1996.1
-
Dynamics of the three methionyl side chains of Streptomyces subtilisin inhibitor. Deuterium NMR studies in solution and in the solid state Reviewed
A Tamura, M Matsushita, A Naito, S Kojima, KI Miura, K Akasaka
PROTEIN SCIENCE 5 ( 1 ) 127 - 139 1996.1
-
GROUP THEORETICAL-STUDY OF THE RADICAL-CATION OF METHANE - THE EFFECT OF TUNNELING MOTIONS ON THE HYPERFINE INTERACTION
M MATSUSHITA, T MOMOSE, T SHIDA, LB KNIGHT
JOURNAL OF CHEMICAL PHYSICS 103 ( 9 ) 3367 - 3376 1995.9
-
ELECTRON-SPIN-RESONANCE STUDIES OF THE METHANE RADICAL CATIONS ((CH4+)-C-12,13, (CDH3+)-C-12,13, (CD2H2+)-C-12, (CD3H+)-C-12, (CD4+)-C-12) IN SOLID NEON MATRICES BETWEEN 2.5 AND 11 K - ANALYSIS OF TUNNELING
LB KNIGHT, GM KING, JT PETTY, M MATSUSHITA, T MOMOSE, T SHIDA
JOURNAL OF CHEMICAL PHYSICS 103 ( 9 ) 3377 - 3385 1995.9
-
ON THE FLUORESCENCE OF CRYSTALLINE C-60 AT 1.2 K
DJ VANDENHEUVEL, IY CHAN, EJJ GROENEN, M MATSUSHITA, J SCHMIDT, G MEIJER
CHEMICAL PHYSICS LETTERS 233 ( 3 ) 284 - 290 1995.2
-
INTERNAL-ROTATION OF THE METHYL-GROUPS IN THE T-BUTYL RADICAL AS STUDIED BY ESR
S KUBOTA, M MATSUSHITA, T SHIDA, A ABURAQABAH, MCR SYMONS, JL WYATT
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 68 ( 1 ) 140 - 145 1995
-
TRIPLET EXCITATIONS OF CRYSTALLINE C-60 - AN OPTICALLY DETECTED MAGNETIC-RESONANCE STUDY IN ZERO-FIELD
M MATSUSHITA, AM FRENS, EJJ GROENEN, OG POLUEKTOV, J SCHMIDT, G MEIJER, MA VERHEIJEN
CHEMICAL PHYSICS LETTERS 214 ( 3-4 ) 349 - 352 1993.11
-
NOVEL ORGANIC IONS OF HIGH-SPIN STATES .3. ESR AND H-1 ENDOR STUDIES OF A MONOCATION OF M-PHENYLENEBIS(PHENYLMETHYLENE)
M MATSUSHITA, T NAKAMURA, T MOMOSE, T SHIDA, Y TEKI, T TAKUI, T KINOSHITA, K ITOH
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 66 ( 5 ) 1333 - 1342 1993.5
-
NOVEL ORGANIC IONS OF HIGH-SPIN STATES
Y TEKI, T TAKUI, T KINOSHITA, K ITOH, M MATSUSHITA, T NAKAMURA, T MOMOSE, T SHIDA
MOLECULAR CRYSTALS AND LIQUID CRYSTALS 232 271 - 288 1993
-
NOVEL ORGANIC IONS OF HIGH-SPIN STATES .2. DETERMINATION OF THE SPIN MULTIPLICITY OF THE GROUND-STATE AND H-1-ENDOR STUDY OF THE MONOANION OF META-PHENYLENEBIS(PHENYLMETHYLENE)
M MATSUSHITA, T NAKAMURA, T MOMOSE, T SHIDA, Y TEKI, T TAKUI, T KINOSHITA, K ITOH
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 114 ( 19 ) 7470 - 7475 1992.9
-
TRIPLET EXCITATION OF C-60 AND THE STRUCTURE OF THE CRYSTAL AT 1.2-K
EJJ GROENEN, OG POLUEKTOV, M MATSUSHITA, J SCHMIDT, JH VANDERWAALS, G MEIJER
CHEMICAL PHYSICS LETTERS 197 ( 3 ) 314 - 318 1992.9
-
THE INSIGNIFICANCE OF THE INTERFERENCE OF CFCL2 RADICAL IN THE ESR STUDY OF RADICAL CATIONS USING THE CFCL3 MATRIX
T SHIDA, T KATO, T MOMOSE, M MATSUSHITA
JOURNAL OF CHEMICAL PHYSICS 95 ( 6 ) 4725 - 4726 1991.9
-
NOVEL ORGANIC IONS OF HIGH-SPIN STATES - ESR DETECTION OF A MONOANION OF META-PHENYLENEBIS(PHENYLMETHYLENE)
M MATSUSHITA, T MOMOSE, T SHIDA, Y TEKI, T TAKUI, K ITOH
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 112 ( 12 ) 4700 - 4702 1990.6
-
INTERNAL-ROTATION OF THE METHYL-GROUP IN THE RADICAL CATION OF DIMETHYL ETHER
M MATSUSHITA, T MOMOSE, T SHIDA
JOURNAL OF CHEMICAL PHYSICS 92 ( 8 ) 4749 - 4758 1990.4
-
MOLECULAR-MOTION OF THE RADICAL CATIONS IN THE CONDENSED PHASE AS STUDIED BY ESR - DIAZABENEZENES
M MATSUSHITA, T MOMOSE, T KATO, T SHIDA
CHEMICAL PHYSICS LETTERS 161 ( 4-5 ) 461 - 465 1989.9
Books
-
Spectroscopy of individual photosynthetic pigment-protein complexes
in Single-molecule detection in solution: methods and applications Ch. Zander, J. Enderlein, and R. A. Keller, eds. Wiley-VCH Verlagsgesellshaft (Weinheim) 2002
-
Spectroscopy of individual photosynthetic pigment-protein complexes
in Single-molecule detection in solution: methods and applications Ch. Zander, J. Enderlein, and R. A. Keller, eds. Wiley-VCH Verlagsgesellshaft (Weinheim) 2002
-
Coherent Raman Spectroscopy: Optical-RF Double Resonance of La around Pr^3+^ in LaF_3_
29th AMPERE - 13th ISMAR Conference (Berlin, Germany) D. Ziessow, W. Lubitz, and F. Lendzian, Eds. 1998
-
Coherent Raman Spectroscopy: Optical-RF Double Resonance of La around Pr^3+^ in LaF_3_
29th AMPERE - 13th ISMAR Conference (Berlin, Germany) D. Ziessow, W. Lubitz, and F. Lendzian, Eds. 1998
-
Radical Cations of Aliphatic Ethers. A Case Study by ESR and Theoretical Analyses
in Radical Ionic Systems A. Lund and M. Shiotani eds. Kluwer Academic Publishers (The Netherlands) 1991
-
Radical Cations of Aliphatic Ethers. A Case Study by ESR and Theoretical Analyses
in Radical Ionic Systems A. Lund and M. Shiotani eds. Kluwer Academic Publishers (The Netherlands) 1991
MISC
-
8aAQ-1 Single-molecule spectroscopy of photosynthetic reaction center protein at 6 K
Kondo Toru, Mutoh Risa, Kurisu Genji, Oh-oka Hirozo, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 69 ( 2 ) 201 - 201 2014.8
-
Spectroscopy of Single Praseodymium Ion towards Observation of Single Nuclear Spin in Solid
Nakamura Ippei, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 68 ( 2 ) 133 - 133 2013.8
-
27pEJ-12 Single-molecule Three Dimensional Confocal Microscopy at 1.5K
Maruo M., Inagawa H., Matsushita M., Fujiyoshi S.
Meeting abstracts of the Physical Society of Japan 68 ( 1 ) 814 - 814 2013.3
-
20pFJ-12 An objective with a numerical aperture of 0.8 for single-molecule spectroscopy at cryogenic temperatures
Inagawa Hironori, Maruo Minako, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 67 ( 2 ) 708 - 708 2012.8
-
Single-molecule spectroscopy of a structural change of a protein Invited Reviewed
Oikawa H, Fujiyoshi S, Dewa T, Nango M, Matsushita M
Kobunshi 60 ( 2 ) 80 - 81 2011.2
-
21pTD-5 Towards Optical Observation of Quantum State of Single Nuclear Spin in Solid
Nakamura I., Yoshihiro T., Fujiyoshi S., Matsushita M.
Meeting abstracts of the Physical Society of Japan 65 ( 1 ) 195 - 195 2010.3
-
23aHL-3 What causes spectral jump of fluorescence of a single molecule in H_2O matrix at 1.5K?
Shimauchi A., Sakurai A., Hirano M., Fujiyoshi S., Matsushita M.
Meeting abstracts of the Physical Society of Japan 65 ( 1 ) 793 - 793 2010.3
-
27pSK-9 Towards Optical Detection of Single Pr^<3+> in Solid at Low-Temperature
Yoshihiro T., Hirano M., Fujiyoshi S., Matsushita M.
Meeting abstracts of the Physical Society of Japan 64 ( 1 ) 144 - 144 2009.3
-
23pYD-5 Improvement of Spherical and Chromatic Aberrations of Reflecting Objective for a Temperature of 1.5 K
Fujiwara Masanori, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 63 ( 1 ) 358 - 358 2008.2
-
23pYD-4 Single molecule study of energy transfer between photosynthetic antenna complexes
Furuya Y, Uchiyama D, Fujiyoshi S, Matsushita M, Suemori Y, Dewa T, Nango M
Meeting abstracts of the Physical Society of Japan 63 ( 1 ) 358 - 358 2008.2
-
23pYD-6 Low-Temperature Single-Protein Spectroscopy performed by Two-Photon Fluorescence Detection
Fujiyoshi S., Matsushita M.
Meeting abstracts of the Physical Society of Japan 63 ( 1 ) 358 - 358 2008.2
-
21aRK-3 Spectroscopy of aggregate of photosynthetic antenna complexes in lipid bilayer
Uchiyama D., FUruya Y., Fujiyoshi S., Matsushita M., Dewa T., Nango M.
Meeting abstracts of the Physical Society of Japan 62 ( 2 ) 348 - 348 2007.8
-
21aZB-13 Scattering Imaging in liquid helium by single-piece reflecting objective
Fujiwara Masanori, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 62 ( 1 ) 175 - 175 2007.2
-
21aZB-13 Scattering imaging in liquid helium by single-piece reflecting objective
Fujiwara Masanori, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 62 ( 1 ) 760 - 760 2007.2
-
23pRC-1 Structure of photosynthetic antenna complex in micelle and in lipid bilayer
Uchiyama D., Fujiyoshi S., Matsushita M., Suemori Y., Dewa T., Nango M.
Meeting abstracts of the Physical Society of Japan 61 ( 2 ) 247 - 247 2006.8
-
28pPSB-55 Spectral diffusion of Individual Light-Harvesting 2 Complex at Cryogenic Temperature
Oikawa H., Fujiyoshi S., Matsushita M., Suemori Y., dewa D., Nango M.
Meeting abstracts of the Physical Society of Japan 61 ( 1 ) 374 - 374 2006.3
-
22pPSA-29 Multiphoton-Fluorescence Microscope Developed for Single-Protein Observation
Fujiyoshi Satoru, Kim Changman, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 60 ( 2 ) 258 - 258 2005.8
-
21aXG-4 Fano-like absorption lineshape of Light-Harvesting antenna complexes
Kato Taro, Fujiyoshi Satoru, Matsushita Michio
Meeting abstracts of the Physical Society of Japan 60 ( 2 ) 239 - 239 2005.8
-
27aPS-93 Fluorescence Excitation Spectra of Individual Light-Harvesting Complex 2 : Temperature Dependence between 5 and 40 K
Oikawa H., Kim C., Matsushita M., Suemori Y., dewa D., Nango M.
Meeting abstracts of the Physical Society of Japan 60 ( 1 ) 338 - 338 2005.3
-
14aPS-75 Protein Structural Fluctuation at Cryogenic Temperature Single-Molecule Study
Oikawa H., Kato T., Kim C, Matsushita M
Meeting abstracts of the Physical Society of Japan 59 ( 2 ) 256 - 256 2004.8
-
27aXS-3 Single Molecule Spectroscopy on Light-Harvesting Complex
Hoshino H., Oikawa H., Kato T., Matsushita M.
Meeting abstracts of the Physical Society of Japan 59 ( 1 ) 149 - 149 2004.3
-
30aPS-88 Low-Temperature Confocal Microscope for Single-Molecule Spectroscopy
Kato T., Oikawa H., Hoshino H., Uchiyama D., Kim C., Matsushita M.
Meeting abstracts of the Physical Society of Japan 59 ( 1 ) 776 - 776 2004.3
-
Unraveling the electronic structure of individual photosynthetic pigment-protein complexes
AM van Oijen, M Ketelaars, M Matsushita, J Kohler, TJ Aartsma, J Schmidt
BIOPHYSICAL JOURNAL 80 ( 1 ) 151A - 151A 2001.1
-
24aYB-11 NMR by coherent Raman scattering of a linear triplet exciton
Matsushita Michio, Kato Tatsuhisa
Meeting abstracts of the Physical Society of Japan 54 ( 2 ) 627 - 627 1999.9
-
Coherent Raman optical-rf double resonance spectroscopy in the condensed phase
MATSUSHITA Michio, KATO Tatsuhisa
Meeting abstracts of the Physical Society of Japan 53 ( 2 ) 271 - 271 1998.9
-
Optical-Optical Double Resonance Spectroscopy by using two Phase-locked Lasers III
KUMAGAI Motohiro, KANAMORI Hideto, MATSUSHITA Michio, KATO Tatsuhisa
Meeting abstracts of the Physical Society of Japan 53 ( 2 ) 887 - 887 1998.9
-
Optical-Optical Double Resonance Spectroscopy by using two Phase-locked lasers II
KUMAGAI Motohiro, KANAMORI Hideto, MATSUSHITA Michio, KATO Tatsuhisa
Meeting abstracts of the Physical Society of Japan 53 ( 1 ) 278 - 278 1998.3
Research Projects
-
タンパク質複合体の１分子観察による構造機能の多様性に関する研究
Grant number：24K01431 2024.4 - 2027.3
日本学術振興会 科学研究費助成事業 基盤研究(B)
松下 道雄
Grant amount：\18460000 （ Direct Cost: \14200000 、 Indirect Cost：\4260000 ）
-
Cryogenic single-molecule fluorescence imaging for deep inside of cell
Grant number：20K21168 2020.7 - 2022.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Matsushita Michio
Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）
Recently, we have been developing the cryogenic fluorescence microscope for nanometer-level imaging (cryo-nanoscope). Although the cryo-nanoscope could be localized the small biomolecules with a nanometer precision, the field-of-view was as small as the size of cell. Here we study a light-sheet excitation for cryo-nanoscope. We examine the two types of the light-sheet excitation methods by wave optical simulation. As a result, the light-sheet excitation using a two objective lens having a NA of 0.6 was much better than that using a one objective lens.
-
タンパク質の機能発現における水素結合の役割に関するクライオ１分子観察による研究
Grant number：19H02672 2019.4 - 2023.3
日本学術振興会 科学研究費助成事業 基盤研究(B)
松下 道雄, 伊関 峰生
Grant amount：\17290000 （ Direct Cost: \13300000 、 Indirect Cost：\3990000 ）
生化学反応は複数の生体分子がつくる柔らかく複雑な系である。それにも関わらず、高い選択性で効率よく進む。このような反応には水素結合が関わっており、水素結合がつくるネットワークが、このような特異性を産み出していると考えられる。しかし、その実体はよく分かっていない。研究を困難にしている主な原因は、（１）反応に関わる水素結合以外にも無数の水素結合があるため、重要な働きをする水素結合を特定できず、（２）様々な準安定状態の平均が観測されるため、活性に重要な変化が埋もれてしまうからである。そこで、応募研究では、水素結合とタンパク質活性化の関係を研究するモデル系として、光活性化酵素(OaPAC, Oscillatoria acuminata Photoactivated adenylyl cyclase)を用い、そのクライオ１分子観察をおこなうことを目標としている。
本年度は水素結合状態を正しく知るための蛍光分子の開発をおこなってきた。その結果、Cy7に代表されるような近赤外蛍光性のシアニン色素を用いると、450 - 600 nmの波長領域の発色団の吸収スペクトルを発光励起スペクトルとして観測できることを明らかにした。OaPACへの蛍光標識が予想以上に難しく、来年度でOaPACの研究をまとめることが困難になってきたが、この系を用いて、OaPAC以外の光捕集系タンパク質の研究も同時におこなっていきたいと考えがえている。具体的には、これまで我々が１分子分光の実績があるLight Harveting Complexや、これまで観察をしたことが無いフィコビリソームなどである。どちらも天然状態で発色団を持っており、この発色団周りの水素結合に関する研究を行う予定である。 -
Multicolor imaging system for visualizing biomolecules in cells
Grant number：18K19054 2018.6 - 2020.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Matsushita Michio
Grant amount：\6240000 （ Direct Cost: \4800000 、 Indirect Cost：\1440000 ）
In 2017, we developed cryogenic fluorescence microscope equipped with off-focusing imaging system. The microscope have localized individual fluorophores with angstrom spatial precision. However, the off-focusing imaging is not suitable for a cellular imaging because of a strong autofluorescence of cells. In the present project, we have developed a new cryogenic fluorescence microscope equipped with mutifocus imaging system. We demonstrated the mutifocus imaging of an individual fluorophore. The three dimensional position was localized with 1 nm precision. The experimental precision is 1.5 times of the theoretical limit.
-
Optical Detection of spin states of individual ions
Grant number：15H03765 2015.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Michio Matsushita
Grant amount：\16900000 （ Direct Cost: \13000000 、 Indirect Cost：\3900000 ）
In the present project, we demonstrated two experimental techniques. First, we demonstrated a solid-immersion-lens (SIL) microscope to improve thel resolution and the detection solid angle. We experimentally showed that by using the SIL, the resolution and the detection solid angle were improved by a factor of 2 and 3, respectively. Second, we demonstrated nanometer-accuracy fluorescence microscopy of individual two-color emitters. Depending on the orientation of the transition dipoles of the emitters with respect to the focal plane, their spots were spheroids whose major axes tilted from the axial direction. When acquired with the axial uncertainty of a conventional fluorescence microscope (700 nm), the tilt due to the dipole orientation caused a lateral shift of 20 nm. Improving the axial uncertainty to 18 nm enabled visualization of these two emitters with a 1-nm accuracy. The technique is necessary to accurately determine the 3D position of multiple rare-earth ions in crystal.
-
Optical observation of nuclear spin states of a single ion
Grant number：23350004 2011.11 - 2014.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
MATSUSHITA Michio
Grant amount：\9750000 （ Direct Cost: \7500000 、 Indirect Cost：\2250000 ）
Nuclear spin of rare-earth (RE) ion doped in an inorganic crystal is a solid-state system useful for processing quantum information. In order to utilize RE ions to build an integrated system of an individual spin qubits, the nuclear spins must be individually detected and controlled. However, only a few experiments were successful in resolving nuclear spin states of single RE ion in solid. The major obstacles in photo-luminescence detection of a single ion with a microscopic setup are weak emission of the ion and background emission from the whole crystal. One of the solutions is to use a crystal smaller than the focal volume. Here we took a different approach to develop a single-component aspheric reflecting objective. Owing to a larger solid-angle of a smaller focal volume, the SN ratio improved to a single-ion level.
-
Structure and function relationship of enzymes studied by single-molecule spectroscopy at liquid-helium temperature
Grant number：19056011 2007 - 2011
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas
MATSUSHITA Michio, FUJIYOSHI Satoru
Grant amount：\35000000 （ Direct Cost: \35000000 ）
Physical and chemical properties of enzyme can be studied using a homogeneous solution of a purified sample of enzyme. However, in living cells, individual protein molecules of enzyme are in different locations to experience different environments, so that they function differently. For understanding enzymatic reactions on the basis of molecular science, observation of individual molecules is essential. In this study we realized spectroscopy of visible-fluorescent single protein molecules.
-
Structural Fluctuation of PhotosyntheticAntenna Complexes Studied by Single-Molecule Spectroscopy
Grant number：16340121 2004 - 2006
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
MATSUSHITA Michio
Grant amount：\16500000 （ Direct Cost: \16500000 ）
A protein has numerous meta-stable structures. In the physiological conditions. The structural fluctuation was pointed out for long years to be important, because the structural fluctuation plays essential role in regulating function of proteins. However, there have been no methods to elucidate the structural dynamics. To break through, we are aiming at getting structural information of single proteins by performing optical spectroscopy of individual proteins. Because measuring a fluorescence spectrum takes at least several milli-seconds, troscopic measurements are performed at temperatures low enough for the structural dynamics to be followed.
In this study we have succeeded to follow one pigment-protein complex to know the temperature dependence of structural dynamics. The experimental data, which are the very first data on the globe show clearly that substantial part of the structural movement is temperature-independent tunneling motion [Oikawa, et. al. J. Am. Chem. Soc. 130(20008)48501.].
Spectroscopy at low temperature of single proteins has been limited to the near infrared region. The method was successfully applied to photosynthetic antenna complexes of photosynthetic bacteria. Nevertheless it is worth making an effort to extend the wavelength of the setup to cover the visible region, since many enzymes absorb or emit in the visible. In order to reduce the chromatic aberration of the objective, which is immersed into liquid helium together with a sample, we have developed a reflecting objective [Fujiyoshi, et. al. Appl. Phys. Lett. 91 (2007) 051125.]. With the reflecting objective we have made two-photon excitation and measured fluorescence spectrum of single GFPs. The spectra were taken in the background-free condition, and series of spectra of the same protein revealed structural dynamics of the protein [Fujiyoshi, et. al. Phys. Rev. Lett. 100 (2008) 168101.]. -
単一分子分光を用いた一個の核スピンの量子状態の観測
Grant number：16655003 2004 - 2005
日本学術振興会 科学研究費助成事業 萌芽研究
松下 道雄
Grant amount：\3700000 （ Direct Cost: \3700000 ）
固体中の一個の核スピンの量子状態を光を使って読み出そうという実験に向けて、初年度は液体ヘリウム用の共焦点顕微蛍光分光装置をつくった。集光効率を重視して作成したが微弱蛍光検出の感度が、系として考えているLaF_3中のPr^<3+>イオンを検出するには少なくともあと一桁足らないことが分かった。製作した低温用の顕微分光装置で集光効率にとって最も重要で、しかも通常の室温用の装置に比べて著しく劣っているのが対物レンズなので、市販の顕微鏡用対物レンズの集光率に近づけるべく、液体ヘリウム温度で使える明るい対物レンズの開発に取り組んだ。主な問題点は組みレンズが使えないので色収差を消せないこと、液体ヘリウム(屈折率1.025)を考慮していないため結像能が悪いことである。屈折系ではなく反射系を用いることを検討した結果、レンズ製造の工作精度で技術的に可能な反射型対物レンズの設計を完成させることができた。
ここで系に要求される発光特性について一考しておく。一個のイオンから発せられる微弱な蛍光を利用する実験なのでもっと良く光る系を選ぶべきであるが、今回の実験では以下のような制約がある。即ち良く光るということは励起状態の寿命が短く、光を吸って励起状態へ移るとすぐに光子を放出して次の光を吸えるようになることなのだが、核スピンの状態による磁気的エネルギーの違いを共鳴光の周波数の差として区別できる程度には寿命が長くなければならない。
実験の目的に完全に合致した理想的なパラメータを持つ系が現実には存在しないが、理論的上限を知ることは実験を進める上で重要である。Pr^<3+>イオンを例に取ると、基底状態と励起状態における核四極子分裂の差は10MHz程度、励起状態^3Poの寿命は47μsである。スペクトルの寿命幅Δν(半値全幅fwhm)と寿命τとの関係はπΔντ=1であるからPr^<3+>イオンの寿命幅はおよそ10kHzである。核スピン状態をスペクトルで分離するには線幅の10倍の分裂が必要とすると、理論的限界までには数十倍から百倍程度のゆとりがある。 -
光磁気二重共鳴を用いたPr^<3+>の状態選択NMRによるLaF_3の結晶場の研究
Grant number：08740471 1996
日本学術振興会 科学研究費助成事業 奨励研究(A)
松下 道雄
Grant amount：\1000000 （ Direct Cost: \1000000 ）
Pr^<3+>の電子励起状態^3P_0は全角運動量J=0で真空中では核四重極子相互作用を持たない。しかし結晶中では結晶場の影響により核四重極子分裂をおこすため、結晶中での核四重極子分裂を調べることによりイオンの周囲の環境についての情報が得られる。LaF_3結晶中のPr^<3+>について、^3P_0からの発光を利用した光とラジオ波の二重共鳴分光を行った。単色のcwレーザー光をPr^<3+>の^3P_0への遷移に共鳴させてラジオ波を掃引する。励起状態内で磁気遷移を起こした原子からの発光は励起光と磁気遷移の分だけエネルギーがずれており、この発光を励起光と重ねると2つの光の周波数の差で強度に変調が生じる。この強度変調を検出することにより^3P_0状態の核四重極子遷移を測定した(ラマンヘテロダイン法)。核四重極子分裂はフォトンエコーの実験からは0.73MHzと1.12MHzと報告されているが、今回のcwの実験では全く異なった結果を得た。フォトンエコーの結果が示す1MHz近傍は、レーザー由来のノイズが大きいものの目だった遷移は見らず、2MHzより上の領域に多数の遷移が現れる。核スピンI=5/2のPr核の核四重極子遷移は2本なので、この2本の遷移が何らかの環境との相互作用でさらに分裂しているか、あるいは結晶中に複数の環境が存在することが考えられる。吸収スペクトルは結晶中の複数の環境の存在の可能性を示唆しているが、磁気遷移の励起波長依存性から少なくとも磁気的環境は単一であることが分かった。磁気的パラメータの情報を増やすために外部磁場中でのゼーマン分裂の測定を行ってその解析を進めており、この解析から相互作用についての手掛かりが得られると考えている。
-
Characterization of Fine Structures in Electronic States by Raman Heterodyne Detection Using Optical Transition.
Grant number：07454158 1995 - 1996
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
KATO Tatsuhisa, MATSUSHITA Michio
Grant amount：\8100000 （ Direct Cost: \8100000 ）
The quadrupole transitions of ^<139> La nuclei around Pr^<3+> doped in LaF_3 have been detected as an optical-rf induced coherent Raman beat on the Pr^<3+>^3H_4-^3P_0 transition (20925 cm^<-1>) at 1.5 K.Because the effective ^<141>Pr nuclear magnetic moment is smaller in the excited ^3P_0 state than in the ground state, the Pr-La interaction decreases upon Pr^<3+> optical excitation, which makes the La quadrupole transitions couple with the Pr optical transition. Four magnetically inequivalent La nuclei were observed, revealing different local environments of La atoms around impurity Pr^<3+> ions.
-
ラマンヘテロダイン法によるC60単結晶の光磁気二重共鳴
Grant number：07740473 1995
日本学術振興会 科学研究費助成事業 奨励研究(A)
松下 道雄
Grant amount：\1100000 （ Direct Cost: \1100000 ）
凝縮相中で光磁気二重共鳴を行うためにラマンヘテロダイン検出法を用いた装置を立ち上げた。光とラジオ波による二重共鳴によって、準位1から2へ光遷移し電子励起状態内で準位2から3へ磁気遷移するとき、あるいは基底状態内で準位1から2へ磁気遷移し準位2から電子励起状態3へ光遷移するとき、選択則が許せば終状態3から始状態1への発光が起こる。この発光はレーザー光とはラジオ波の周波数分だけ周波数がずれているため、試料を透過したレーザー光にこの発光が重なると、透過光強度がラジオ波の周波数で変調を受ける。微弱な発光を透過光強度のラジオ波帯域の変調信号として感度よく捕えようというのが本研究で用いたラマンヘテロダイン法である。装置は、可視領域の波長可変単一モードレーザー、試料1.5Kの極低温に保つ液体ヘリウムクライオスタット、磁気遷移を起こさせるためのラジオ波源、そしてヘテロダインビ-ト信号を検出する高速のフォトダイオードからなる。LaF3結晶中にドープされたPr3+イオンの1D2状態と基底3H4状態の間の光遷移にレーザー光を共鳴させ、電子励起、基底両状態についてPr核の核四重極子遷移を観測した。ラジオ波の検出の際、入力信号との位相の相関をとることにより、レーザー光や検出器のもつ雑音を除去できることを示した。今後は、二重共鳴のもつ選択性を生かして、C60単結晶のように結晶中に複数のサイトがある系について吸収波長の違いを利用して各々のサイトを選択して磁気共鳴を行う、などの応用をめざす。
-
光検出磁気共鳴の新しい手法の試み-ラマンヘテロダインビ-ト法によるC_<60>単結晶の励起三重項状態の分光-
Grant number：06740463 1994
日本学術振興会 科学研究費助成事業 奨励研究(A)
松下 道雄
Grant amount：\900000 （ Direct Cost: \900000 ）
標題のラマンヘテロダインビ-ト検出のESRを行うためには、1.極低温の試料に 2.単色光と 3.ラジオ波を印加し、4.前方散乱光に含まれる数百MHzの微弱なビ-ト信号を検出しなければならない。本年度はこれら4つの条件のうち1から3を達成し、4については改善の後、来年度には本測定ができる状況に達した。以下に本年度の進渉状況を具体的に述べる。
1.試料を極低温に冷やすために液体ヘリウムのクライオスタットを設計、制作した。さらに液体ヘリウム容器を数Torrに減圧することにより、1.3Kの超流動ヘリウムを得た。クライオスタットの液体ヘリウム容量は5リッターで、1.3Kを10時間維持できる性能を持つ。
2.単色光はArイオンレーザー励起のTi:Sapphireリングレーザーを用い、波長700〜1000nmで単一モード発振できる。今年度、発振波長を知るための波長計、単一モード発振を確認するためのスペクトラムアナライザーを導入した。以上、1と2の条件が揃ったので予備的測定としてC_<60>単結晶の1.3Kにおける発光スペクトルを測定した。
3.ラジオ波を試料に効率好く照射するために、容量可変のコンデンサーとコイルの直列共振器を制作した。共振周波数は90〜200MHzの範囲で変化させることができ、Q値は150であった。ラジオ波が実際試料に印加されていることは、1.3KでC_<60>単結晶のODMR信号を測定することによって確認した。
4.1GHzの帯域を持つシリコン光検出器の出力の中から、ラジオ波と同じ周波数を持つビ-ト成分をスペクトラムアナライザーで検出する。現在、予備的な測定で、数mWの定常光に含まれる10_<-6>の強度のビ-ト成分を検出できることが分かった。実際の測定にはもう数十倍の感度の向上が必要であり、次年度にラジオ波の小信号増幅器を購入して本測定を行う。